CN105917913A - Method for breeding mycorrhizal seedlings by truffles and quercus acutissima - Google Patents
Method for breeding mycorrhizal seedlings by truffles and quercus acutissima Download PDFInfo
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- CN105917913A CN105917913A CN201610272291.9A CN201610272291A CN105917913A CN 105917913 A CN105917913 A CN 105917913A CN 201610272291 A CN201610272291 A CN 201610272291A CN 105917913 A CN105917913 A CN 105917913A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Abstract
The invention belongs to the field of agricultural seedling raising, relates to a method for breeding mycorrhizal seedlings by truffles and quercus acutissima and particularly relates to a breeding method which can be used for obtaining truffle mycorrhizal seedlings through inoculating the quercus acutissima by effectively using truffle infection. The breeding method comprises the following steps: (1) preparing young quercus acutissima seedlings; (2) inoculating the truffles to the young quercus acutissima seedlings; and (3) carrying out adult seedling management. The method has the keys that the quercus acutissima is inoculated with the truffles, so that the development of roots of the quercus acutissima is facilitated, and the resistance and metabolic strength of the quercus acutissima can be improved; and after inoculation, the chlorophyll content, SOD enzyme activity and root activity of plants are all improved remarkably, the occurrence of roots is promoted, the ratio of an overground part to an underground part is increased, and the survival of the plants is facilitated. Through breeding the mycorrhizal seedlings by the method disclosed by the invention, a higher mycorrhiza infection rate can be obtained; and ingredients such as organic, water retention and inorganic ions are added into a microbial agent, so that the infection index of mycorrhizae is increased by using the characteristics such as hydrotropism and fertilizer tropism of plant seedlings.
Description
Technical field
The invention belongs to agricultural cultivation field, relate to a kind of method that Truffe cultivates Va Mycorrhiza Seedling with Quercus acutissima, specifically utilize Truffe sense
Dye inoculation Quercus acutissima obtains the breeding method of Truffe Va Mycorrhiza Seedling.
Background technology
Truffe (Truffle) belongs to Rare edible fungus, is ascomycetous mycelia and fine-root symbiosis and the ectotrophic mycorrhiza that formed, extensively
It is distributed in the Temperate Region in China of the earth, and in the range of Chinese yunnan and Panxi Area, Sichuan Province most area are positioned at this, Truffe ascocarp
Expensive, it is a kind of plant with higher economic worth.Owing to the edible ascocarp of Truffe is born in underground, seek to the mankind
Look for and gather in the crops Truffe and bring great difficulty, for meeting interests needs, just occur in that the phenomenon that unordered and transition is excavated, it is seen that realize
Truffe artificial culture's is significant, and Truffe ascocarp to be obtained is necessary for first cultivating Truffe Va Mycorrhiza Seedling, under the conditions of now,
The conventional pinaceae plant such as Pinus armandi Franch-P. Komavovii Lavl., Pinus massoniana Lamb and oak plant cultivate its Va Mycorrhiza Seedling, when environmental condition is suitable, from nursery to inspection
Measure mycorhiza and preliminarily form at least cost 6 months, and usually because a variety of causes causes infection rate to differ.
Quercus acutissima (Quercus acutissima Carruth) belongs to Fagaceae, oak plant, and all there is distribution in China manyly, is a kind of tool
There is the plant of higher economic worth.But rarely seen Truffe infects inoculation Quercus acutissima carries out the report of root cultivation.The present invention is intended to provide a kind of
Truffe infects inoculation Quercus acutissima and obtains the breeding method of Truffe Va Mycorrhiza Seedling, and screening draws optimum inoculation amount and vaccination ways.
Summary of the invention
Technical problem solved by the invention be to provide a kind of can effectively utilize Truffe infect inoculation Quercus acutissima obtain Truffe Va Mycorrhiza Seedling
Breeding method.
The breeding method of Truffe Va Mycorrhiza Seedling of the present invention, comprises the following steps: (1) prepares Quercus acutissima seedling；(2) Quercus acutissima seedling inoculation
Truffe；(3) seedling management.
In technique scheme, step (1) described preparation Quercus acutissima seedling is: collects or cultivates and obtains Quercus acutissima aseptic seedlings；
In technique scheme, it is to obtain sterile culture, Seedling by the way of gathering or buying that described collection obtains Quercus acutissima seedling
Age is 2～the Quercus acutissima seedling in April.
In technique scheme, described cultivation obtains Quercus acutissima seedling for being 2～4 by sterile culture mode by cultivating seeds to seedling age
The Quercus acutissima seedling of the moon.
In technique scheme, it is as follows that described cultivation obtains the method that Quercus acutissima seedling preferably employs:
1) fresh and healthy of gathering Quercus acutissima seed, with disinfectant solution seed soaking sterilization then by sterile water wash after water flotation kind, then by fiber crops
Oak seed sterilizing river sand sand hides 3～5 months；
2) sow to next spring or manual control environment's temperature reaches 18-28 DEG C and sows, by the Quercus acutissima screen for seeds of Sha Zanghou
Sterilization after going out and being selected；
Wherein, step 2) described sterilization method is: Quercus acutissima seed distilled water is cleaned, then with the acid that concentration is 0.1%～0.4%
Property potassium permanganate solution soak seed 0.5～2h, after lose eluate colour developing with sterile water wash seed to naked eyes, then use sterilized water
Seed is soaked 12～36h, and whole disinfecting process can not introduce other miscellaneous bacteria；
3) Quercus acutissima planting seed is in aseptic seedling medium, spacing 0.5～1.2cm between kind, after sowing, seals with preservative film
Device for raising seedlings, carries out thermophilic accelerating germination, takes off film nursery after germination percentage reaches more than 50%, and seedling seedling age reaches 2～April, Ji Keyi
Plant.
Wherein, step 1) the husky Tibetan condition of described river sand is: husky temperature of hiding is 0～4 DEG C, and humidity is natural.
Wherein, step 1) described disinfectant solution is the hydrogen peroxide of 15～25%.
Wherein, step 1) described disinfecting time is 0.5～1h.
Wherein, step 1) sterilization method of river sand of described sterilizing cooling is high-temperature heat sterilization: 121～126 DEG C, 2h；River sand
Water content 15%～30%.
In technique scheme, either collecting gained and still cultivate gained, gained Quercus acutissima seedling requires at the nursery base without miscellaneous bacteria
Matter is cultivated.
Preferably, when cultivating Quercus acutissima seedling, cultivating described seedling medium is the component formed by following volume proportion: Vermiculitum: Yuexi
Soil: water=1:1:0.2～0.5.
In technique scheme, described Quercus acutissima seedling need to carry out suitable mechanical trauma, is beneficial to plant root tissue and quickly infects
Truffe, promotes the quick formation of mycorhiza, and main root, part side tip of a root end cut 1.0～2.0cm.
In technique scheme, step (2) Quercus acutissima seedling inoculation Truffe is；
A, making Truffe sporophore microbial inoculum；
B, Quercus acutissima seedling seedling age reach 2～during April, transplant to transplanting medium, inoculate Truffe sporophore microbial inoculum while transplanting.
In technique scheme, described in step A, Truffe sporophore microbial inoculum is made up of the component of following weight proportioning: peat: carbonic acid
Calcium: water-retaining agent: Truffe ascospore powder=3～5:1～1.5:1:1～5.
In technique scheme, transplanting medium described in step B is the component formed by following volume proportion: peat: Vermiculitum: Yuexi
Soil: river sand: calcium carbonate=1.5～3:1～2.5:2.5:1～3:1；Described transplanting medium water content 30～60%, pH is 7.0～7.5, and sterilizing is i.e.
?.
In technique scheme, the sterilizing in described transplanting medium is high-temperature heat sterilization, and sterilising conditions is 95～110 DEG C, 4～6h.
In technique scheme, described peat is Germany's thin peat of import；Described calcium carbonate is the powder carbonic acid after limestone calcination
Calcium；Described Truffe ascospore powder is that to pulverize after Truffe ascocarp lyophilizing be the powder after 50 mesh sieve；Described water-retaining agent is agricultural
Water-retaining agent.Described agricultural water-retaining agent is super absorbent resin, is to have higher water absorption energy and the high molecular polymer of water retention property
General name.Agricultural water-retaining agent stores medicament or miniature reservoir also known as agent for preserving soil moisture, antiperspirant, storage fertilizer, not only has high suction
Aqueous energy, also has water conservation water-releasing, moisture is supplied to plant and utilizes.
In technique scheme, step B inoculation Truffe sporophore microbial inoculum, it is 0.5～2.5g that Truffe is effectively inoculated in individual plant list cave.
In technique scheme, after described in step B, inoculation is mixed homogeneously with a small amount of transplanting medium for Truffe sporophore microbial inoculum, place
In bottom is lined with the seedlings nursing plate of a small amount of transplanting medium, after then making Quercus acutissima seedlings root and being fully contacted with the substrate being mixed with microbial inoculum,
Fix seedling with transplanting medium again, fill transplanting medium.
In technique scheme, the management of step (3) seedling is:
A, to transplant inoculation complete and irrigate and determine root water；
B, holding transplanting medium water content 45 ± 5%, room temperature 20～30 DEG C, relative air humidity 70 ± 5%, illumination 300～600Lux,
Maintaining the circulation of air, the micro-spray of air 2 in a week～4 times, substrate is poured water 1～2 time；
Fertility manages: every month blade face and substrate sprinkling irrigation nutritional solution 1～2 times；
Pest management: place yellow plate or optionally take corresponding measure；Disable high silkworm raiser's medicine.Whole cultivating process is carried out at booth,
Take to leak informaton, lower the temperature, control the measures such as water and seedling is performed physical exercise, so that poor environment can be rapidly adapted to after its field planting.
After transplanting 1 month, inspect 1 mycorhiza formational situation at random by random samples every two weeks, during detection, randomly select 10%, with super
After pure water cleans fraction matrix, it is placed on preliminary observation plant tip of a root mycorhiza formational situation under stereomicroscope, finds mycorhiza, can
Picking out, carry out DNA analysis qualification, sequence alignment works, to determine whether to infect successfully.
Beneficial effects of the present invention:
The present invention uses Quercus acutissima to inoculate Truffe, the beneficially development of Quercus acutissima root system, can improve resistance and the metabolism degree of Quercus acutissima；Connect
After Zhong, the chlorophyll of plant, the work of SOD enzyme, improving activity of root system are remarkably improved, and promote the generation of root system, increase ground
Top and the ratio of underground part, the beneficially existence of plant.Cultivate Va Mycorrhiza Seedling by the inventive method, higher mycorhiza can be obtained and invade
Dye rate, with the addition of the compositions such as organic, water conservation, inorganic ions in microbial inoculum, utilize the hydrotropism of plant seedling, to features such as fertilizer,
Improve the Index of infection of mycorhiza.
Accompanying drawing explanation
Fig. 1 is inoculation cultivation schematic diagram.
Fig. 2 is embodiment 1 mycorrhizal morphology figure.
Wherein, Fig. 2 a represents that root system mycorrhizal morphology, Fig. 2 b represent side root fungus root morphology.
Fig. 3 is embodiment 2 mycorrhizal morphology figure.
Wherein, Fig. 3 c represents that root system mycorrhizal morphology, Fig. 3 d represent the first side root fungus root formational situation, and Fig. 3 e represents the bacterium of cluster
Root, Fig. 3 f represents single mycorhiza Tip Splitting.
Fig. 4 is comparative example 1 mycorrhizal morphology figure.
Wherein, Fig. 4 g represents part Root morphology (without infecting), and Fig. 4 h represents side root (without infecting).
Fig. 5 is comparative example 2 mycorrhizal morphology figure.
Wherein, Fig. 5 i represents root system general morphology and mycorhiza formational situation, and Fig. 5 j represents side root fungus root formational situation.
Detailed description of the invention
The detailed description of the invention of form by the following examples, is described in further detail the foregoing of the present invention again, says
Bright but be not intended to the present invention.
The effective inoculum concentration of embodiment 1 microbial inoculum of the present invention is 1g, and plant cuts each 1cm of taproot and lateral root.
(1) cultivating Quercus acutissima seedling, detailed step is as follows:
Gather November then fresh Quercus acutissima seed, with the hydrogen peroxide seed soaking sterilization 1h of disinfectant solution 20%, river sand after water flotation kind
High-temperature heat sterilization: 121～126 DEG C, 2h；River sand water content 18%；The river sand sand that Quercus acutissima seed cools down with sterilizing is hidden 4 months,
Husky temperature of hiding is 0～4 DEG C, and humidity is natural.To March in the coming year, after being sifted out and be selected by the seed of Sha Zanghou, use ultrapure washing
Only, then soak seed 1h with acid permanganate soln that concentration is 0.2%, after lose to naked eyes with sterile water wash seed and to wash out
Liquid develops the color, then with sterilized water, seed is soaked 24h, and whole disinfecting process can not introduce other miscellaneous bacteria, finally sow at nursery base
Matter (Vermiculitum: Yuexi soil: in water 1:1:0.5 by volume), spacing 1m between kind, after sowing, seals seedlings nursing plate with preservative film,
It is positioned on sterilizing stand (Methylpartricin Sodium Laurylsulfate etc. are disinfected) and carries out thermophilic accelerating germination, treat that germination percentage reaches 50% and is followed by film nursery, seedling
Seedling age reaches March, transplants, and cuts taproot and lateral root each 1.0cm during transplanting.
(2) artificial vaccination:
Select fresh Truffe sporophore in February, according to following weight than mixing peat (German): calcium carbonate: water-retaining agent: India's block
Mushroom cystospore powder=4:1:1:4 makes Truffe sporophore microbial inoculum, be placed on 4 DEG C of refrigerators preserve stand-by.Treat that seedling seedling age reaches March,
Being transplanted in transplanting medium, carry out inoculating work while transplanting, individual plant list cave is effectively inoculated Truffe and is reached 1.0g.Transplant base
Matter is peat: Vermiculitum: Yuexi soil: river sand: calcium carbonate=3:1.5:2.5:2:1, water content 40%, and pH, 7.3, goes out through high temperature is damp and hot
Bacterium, 95～110 DEG C, 4～6h.
Concrete method for transplanting is: require to calculate after the consumption of bacteriostatic agent is mixed homogeneously with a small amount of transplanting medium again according to cultivation,
It is placed on bottom to be lined with in the seedlings nursing plate of a small amount of transplanting medium, after then making Pinus armandi Franch-P. Komavovii Lavl. root system and being fully contacted with the substrate being mixed with microbial inoculum,
Fix seedling with transplanting medium again, fill transplanting medium.
(3) seedling management:
Transplanting inoculation complete and irrigate and determine root water, keep transplanting medium water content 45 ± 5%, room temperature 20～30 DEG C, air is the wettest
Degree 70 ± 5%, illumination 300～600Lux, keep the circulation of shed air, general one week can the micro-spray of air 2～4 times, substrate is poured water
1～2 time.On fertility manages, each month blade face and substrate sprinkling irrigation nutritional solution 1～2 times.Pest management, need to be in booth many places
Place yellow plate, if any other problem, can optionally take corresponding measure, disable high silkworm raiser's medicine.Whole cultivating process enters at booth
OK, while growth of seedling, seedling exercising can also be realized.
Mycorhiza formational situation detects:
After transplanting 2 months, mycorhiza be can be observed and preliminarily form, during to 3 months, can be seen that mycorhiza color has been deepened, root system
Overall infection intensity increases, and the when of to 5th month, it is light brown that part mycorhiza be can be observed, and randomly selects 10% and counts
According to collection.
The effective inoculum concentration of embodiment 2 microbial inoculum of the present invention is 2g, and plant cuts each 1cm of taproot and lateral root.
(1) cultivating Quercus acutissima seedling, detailed step is as follows:
Gather November then fresh Quercus acutissima seed, with the hydrogen peroxide seed soaking sterilization 1h of disinfectant solution 20%, river sand after water flotation kind
For high-temperature heat sterilization: 121～126 DEG C, 2h；River sand water content 18%；The river sand sand that Quercus acutissima seed cools down with sterilizing is hidden 4
Individual month, husky temperature of hiding was 0～4 DEG C, and humidity is natural.To March in the coming year, after the seed of Sha Zanghou is sifted out and be selected, with super
Pure water is cleaned, then soaks seed 1h with the acid permanganate soln that concentration is 0.2%, afterwards with sterile water wash seed to naked eyes not
Seeing that eluate develops the color, then with sterilized water, seed is soaked 24h, whole disinfecting process can not introduce other miscellaneous bacteria, finally sow
(Vermiculitum: Yuexi soil: in water 1:1:0.5 by volume), spacing 1m between kind, after sowing, seals seedling medium with preservative film
Seedlings nursing plate, is positioned on sterilizing stand (Methylpartricin Sodium Laurylsulfate etc. are disinfected) and carries out thermophilic accelerating germination, treats that germination percentage reaches more than 50% and is followed by
Film nursery, seedling seedling age reaches March, transplants, and cuts taproot and lateral root each 1.0cm during transplanting.
(2) artificial vaccination:
Purchase Truffe sporophore in February, according to following weight than mixing peat (German): calcium carbonate: water-retaining agent: tuber indicum
Cystospore powder=4:1:1:4 makes microbial inoculum, be placed on 4 DEG C of refrigerators preserve stand-by.Seedling seedling age to be grown directly from seeds reaches March, is transplanted into shifting
Planting in substrate, carry out inoculating work while transplanting, individual plant list cave is effectively inoculated Truffe and is reached 2.0g.Transplanting medium is peat:
Vermiculitum: Yuexi soil: river sand: calcium carbonate=3:1.5:2.5:2:1, water content 40%, pH is 7.3, through high-temperature heat sterilization, 95～110 DEG C,
4～6h.
Concrete method for transplanting is: require to calculate after the consumption of bacteriostatic agent is mixed homogeneously with a small amount of transplanting medium again according to cultivation,
It is placed on bottom to be lined with in the seedlings nursing plate of a small amount of transplanting medium, after then making Pinus armandi Franch-P. Komavovii Lavl. root system and being fully contacted with the substrate being mixed with microbial inoculum,
Fix seedling with transplanting medium again, fill transplanting medium.
(3) seedling management:
Transplanting inoculation complete and irrigate and determine root water, keep transplanting medium water content 45 ± 5%, room temperature 20～30 DEG C, air is the wettest
Degree 70 ± 5%, illumination 300～600Lux, keep the circulation of shed air, general one week can the micro-spray of air 2～4 times, substrate is poured water
1～2 time.On fertility manages, each month blade face and substrate sprinkling irrigation nutritional solution 1～2 times.Pest management, need to be in booth many places
Place yellow plate, if any other problem, can optionally take corresponding measure, disable high silkworm raiser's medicine.Whole cultivating process enters at booth
OK, while growth of seedling, seedling exercising can also be realized.
Mycorhiza formational situation detects:
After transplanting 2 months, mycorhiza be can be observed and preliminarily form, during to 3 months, can be seen that mycorhiza color has been deepened, root system
Overall infection intensity increases, and the when of to 5th month, it is light brown that part mycorhiza be can be observed, and randomly selects 10% and counts
According to collection.
The effective inoculum concentration of embodiment 3 (blank) microbial inoculum of the present invention is 0g, and plant cuts each 1cm of taproot and lateral root.
(1) cultivating Quercus acutissima seedling, detailed step is as follows:
Gathering fresh Quercus acutissima seed, with the hydrogen peroxide seed soaking sterilization 1h of disinfectant solution 20% after water flotation kind, river sand high temperature is damp and hot to go out
Bacterium: 121～126 DEG C, 2h；River sand water content 18%；The river sand sand that Quercus acutissima seed cools down with sterilizing is hidden 4 months, husky Tibetan temperature
Being 0～4 DEG C, humidity is natural.To March in the coming year, after the seed of Sha Zanghou is sifted out and be selected, clean with ultra-pure water, then use
Concentration be 0.2% acid permanganate soln soak seed 1h, after with sterile water wash seed to naked eyes lose eluate colour developing,
With sterilized water, seed being soaked 24h again, whole disinfecting process can not introduce other miscellaneous bacteria, finally sow seedling medium (Vermiculitum:
Yuexi soil: in water 1:1:0.5 by volume), middle ware, after 1m, sowing, seals seedlings nursing plate with preservative film, is positioned over
Carry out thermophilic accelerating germination on sterilizing stand (Methylpartricin Sodium Laurylsulfate etc. are disinfected), treat that germination percentage reaches more than 50% and is followed by film nursery, seedling Seedling
Age reaches March, transplants, and cuts taproot and lateral root each 1.0cm during transplanting.
(2) transplant
Seedling age reaches the Quercus acutissima seedling cultivation in March enter in porous seedlings nursing plate.
(3) seedling management:
Transplanting inoculation complete and irrigate and determine root water, keep transplanting medium water content 45 ± 5%, room temperature 20～30 DEG C, air is the wettest
Degree 70 ± 5%, illumination 300～600Lux, keep the circulation of shed air, general one week can the micro-spray of air 2～4 times, substrate is poured water
1～2 time.On fertility manages, each month blade face and substrate sprinkling irrigation nutritional solution 1～2 times.Pest management, need to be in booth many places
Place yellow plate, if any other problem, can optionally take corresponding measure, disable high silkworm raiser's medicine.Whole cultivating process enters at booth
OK, while growth of seedling, seedling exercising can also be realized.
Embodiment 4
Implementing number of patent application is 200710048943.1, and described Truffe microbial inoculum makes and be correlated with inoculation method according to its mycorhiza
Seedling cultivating and managing method carries out repeating test, and implements by embodiment 3, final surveying record related data.
Various embodiments of the present invention result is as follows:
Improving activity of root system, SOD enzyme are lived, chlorophyll content is all closely related with the metabolism of plant, can indirectly reflect the health of plant
Situation, concrete manifestation situation see table shown in 1, and mycorrhizal morphology figure is shown in Fig. 2-Fig. 5.
Table 1 each embodiment data determination summary sheet
Note: improving activity of root system measures and uses TTC method to measure；SOD determination of activity uses nitroblue tetrazolium (NBT) method；Root/shoot ratio is
Under ground portion and the ratio of aerial parts dry weight；Measuring chlorophyll content uses plant physiology experiment method to measure；Root system infection rate=
Infection radical in investigation root/investigate total radical.
As shown in Table 1, in the case of not inoculation, the improving activity of root system of Quercus acutissima, SOD enzyme are lived, chlorophyll content is below it
He is not so good as other process groups at group, i.e. metabolic situation, indirectly shows, the development of inoculation truffles, beneficially root system, can improve
The resistance of Quercus acutissima and metabolism degree.As can be known from the table data, strengthen inoculum concentration and can obtain higher infection rate, plant after inoculation
Chlorophyll, SOD enzyme are lived, improving activity of root system is remarkably improved, and promote the generation of root system, increase overground part and underground
The existence of the ratio in portion, beneficially plant.Cultivating Va Mycorrhiza Seedling by the technology of the present invention, can obtain higher Mycorrhizal Infection Incidence, it is invaded
Dye effect becomes apparent from described in patent 200710048943.1, with the addition of the compositions such as organic, water conservation, inorganic ions in microbial inoculum,
Utilize the hydrotropism of plant seedling, to the features such as fertilizer, the Index of infection of raising mycorhiza.
To sum up, the present invention uses Quercus acutissima to inoculate Truffe, the beneficially development of Quercus acutissima root system, and resistance and the metabolism that can improve Quercus acutissima are strong
Degree；After inoculation, the chlorophyll of plant, the work of SOD enzyme, improving activity of root system are remarkably improved, and promote the generation of root system, increase
The big overground part ratio with underground part, the beneficially existence of plant.Cultivate Va Mycorrhiza Seedling by the inventive method, can obtain higher
Mycorrhizal Infection Incidence, with the addition of the compositions such as organic, water conservation, inorganic ions, utilizes the hydrotropism of plant seedling, to fertilizer in microbial inoculum
Etc. feature, improve the Index of infection of mycorhiza.
Claims (9)
1. the method that Truffe cultivates Va Mycorrhiza Seedling with Quercus acutissima, it is characterised in that: comprise the following steps: (1) prepares Quercus acutissima seedling；(2)
Quercus acutissima seedling inoculation Truffe；(3) seedling management；
Wherein, step (2) Quercus acutissima seedling inoculation Truffe is；
A, making Truffe sporophore microbial inoculum；
B, Quercus acutissima seedling seedling age reach 2～during April, transplant to transplanting medium, inoculate Truffe sporophore microbial inoculum while transplanting.
The method that Truffe the most according to claim 1 cultivates Va Mycorrhiza Seedling with Quercus acutissima, it is characterised in that: the step of step (2)
Truffe sporophore microbial inoculum described in rapid A is made up of the component of following weight proportioning: peat: calcium carbonate: water-retaining agent: Truffe ascospore
Powder=3～5:1～1.5:1:1～5.
The method that Truffe the most according to claim 2 cultivates Va Mycorrhiza Seedling with Quercus acutissima, it is characterised in that: at least meet following appointing
One:
Described peat is Germany's thin peat of import；
Described calcium carbonate is the powder calcium carbonate after limestone calcination；
Described Truffe ascospore powder is that to pulverize after Truffe ascocarp lyophilizing be the powder after 50 mesh sieve；
Described water-retaining agent is agricultural water-retaining agent.
The method that Truffe the most according to claim 1 cultivates Va Mycorrhiza Seedling with Quercus acutissima, it is characterised in that: the step of step (2)
Transplanting medium described in rapid B is the component formed by following volume proportion: peat: Vermiculitum: Yuexi soil: river sand: calcium carbonate=1.5～3:
1～2.5:2.5:1～3:1；Described transplanting medium water content 30～60%, pH is 7.0～7.5, sterilizing, thus obtaining the product.
5. the method cultivating Va Mycorrhiza Seedling according to the Truffe described in any one of claim 1-4 with Quercus acutissima, it is characterised in that: the fullest
Be enough to lower any one:
Sterilizing in transplanting medium described in step B of step (2) is high-temperature heat sterilization, and sterilising conditions is 95～110 DEG C, 4～6h；
The step B inoculation Truffe sporophore microbial inoculum of step (2), it is 0.5～2.5g that Truffe is effectively inoculated in individual plant list cave；
After inoculation is mixed homogeneously with a small amount of transplanting medium for Truffe sporophore microbial inoculum described in step B of step (2), it is placed on the end
Portion is lined with in the seedlings nursing plate of a small amount of transplanting medium, after then making Quercus acutissima seedlings root and being fully contacted with the substrate being mixed with microbial inoculum, then uses
Transplanting medium fixes seedling, fills transplanting medium.
The method that Truffe the most according to claim 1 cultivates Va Mycorrhiza Seedling with Quercus acutissima, it is characterised in that: at least meet following appointing
One:
Step (1) described preparation Quercus acutissima seedling is: collects or cultivates and obtains Quercus acutissima aseptic seedlings；
Preferably, described collection obtain Quercus acutissima seedling be obtain by the way of gathering or buying sterile culture, seedling age be 2～4
The Quercus acutissima seedling of the moon；
Preferably, described cultivation obtains Quercus acutissima seedling is by Quercus acutissima that cultivating seeds to seedling age is 2～April by sterile culture mode
Seedling.
The method that Truffe the most according to claim 6 cultivates Va Mycorrhiza Seedling with Quercus acutissima, it is characterised in that: described cultivation obtains fiber crops
Oak seedling method is as follows:
1) fresh and healthy of gathering Quercus acutissima seed, with disinfectant solution seed soaking sterilization then by sterile water wash after water flotation kind, then by fiber crops
Oak seed sterilizing river sand sand hides 3～5 months；
2) sow to next spring or manual control environment's temperature reaches 18-28 DEG C and sows, by the Quercus acutissima screen for seeds of Sha Zanghou
Sterilization after going out and being selected；
3) Quercus acutissima planting seed is in aseptic seedling medium, spacing 0.5～1.2cm between kind, after sowing, seals with preservative film
Living device for raising seedlings, carry out thermophilic accelerating germination, take off film nursery after germination percentage reaches more than 50%, seedling seedling age reaches 2～April,
Transplant.
The method that Truffe the most according to claim 7 cultivates Va Mycorrhiza Seedling with Quercus acutissima, it is characterised in that: at least meet following appointing
One:
Step 1) the husky Tibetan condition of described river sand is: husky temperature of hiding is 0～4 DEG C, and humidity is natural；
Step 1) described disinfectant solution is the hydrogen peroxide of 15～25%；
Step 1) described disinfecting time is 0.5～1h；
Step 1) sterilization method of river sand of described sterilizing cooling is high-temperature heat sterilization: 121～126 DEG C, 2h；River sand is aqueous
Amount 15%～30%；
Preferably, when cultivating Quercus acutissima seedling, cultivating described seedling medium is the component formed by following volume proportion: Vermiculitum: Yuexi
Soil: water=1:1:0.2～0.5；
Step 2) described sterilization method is: Quercus acutissima seed distilled water is cleaned, then with the acid Gao Meng that concentration is 0.1%～0.4%
Acid potassium solution soak seed 0.5～2h, after with sterile water wash seed to naked eyes lose eluate colour developing, then with sterilized water will kind
Son soaks 12～36h；
Step 3) described Quercus acutissima seedling seedling age reaches 2～during April, carries out suitable mechanical trauma；
Preferably, step 3) described Quercus acutissima seedling seedling age reaches 2～during April, and described mechanical trauma is main root, the part side tip of a root
End cuts 1.0～2.0cm.
The method that Truffe the most according to claim 1 cultivates Va Mycorrhiza Seedling with Quercus acutissima, it is characterised in that: step (3) seedling
Management is:
A, to transplant inoculation complete and irrigate and determine root water；
B, holding transplanting medium water content 45 ± 5%, room temperature 20～30 DEG C, relative air humidity 70 ± 5%, illumination 300～600Lux,
Maintaining the circulation of air, the micro-spray of air 2 in a week～4 times, substrate is poured water 1～2 time；
Fertility manages: every month blade face and substrate sprinkling irrigation nutritional solution 1～2 times；
Pest management: place yellow plate or optionally take corresponding measure；Disable high silkworm raiser's medicine.
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