CN103026884A - Artificial planting method for producing truffles at high yield - Google Patents
Artificial planting method for producing truffles at high yield Download PDFInfo
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- CN103026884A CN103026884A CN2012105882023A CN201210588202A CN103026884A CN 103026884 A CN103026884 A CN 103026884A CN 2012105882023 A CN2012105882023 A CN 2012105882023A CN 201210588202 A CN201210588202 A CN 201210588202A CN 103026884 A CN103026884 A CN 103026884A
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- ferfas
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- 241001489212 Tuber Species 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 claims abstract description 8
- 238000011081 inoculation Methods 0.000 claims abstract description 7
- 241001236247 Pinus bungeana Species 0.000 claims abstract description 6
- 238000009331 sowing Methods 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 239000011159 matrix material Substances 0.000 claims description 12
- 238000005406 washing Methods 0.000 claims description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 6
- 239000002068 microbial inoculum Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 4
- 241000195940 Bryophyta Species 0.000 claims description 3
- 239000005708 Sodium hypochlorite Substances 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 230000000249 desinfective effect Effects 0.000 claims description 3
- 239000012467 final product Substances 0.000 claims description 3
- 239000003864 humus Substances 0.000 claims description 3
- 238000002513 implantation Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000003415 peat Substances 0.000 claims description 3
- 239000010451 perlite Substances 0.000 claims description 3
- 235000019362 perlite Nutrition 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 239000004576 sand Substances 0.000 claims description 3
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 3
- 239000002689 soil Substances 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 239000010455 vermiculite Substances 0.000 claims description 3
- 235000019354 vermiculite Nutrition 0.000 claims description 3
- 229910052902 vermiculite Inorganic materials 0.000 claims description 3
- 238000003306 harvesting Methods 0.000 abstract description 3
- 238000011084 recovery Methods 0.000 abstract description 3
- 238000012258 culturing Methods 0.000 abstract 3
- 241000609666 Tuber aestivum Species 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 239000002054 inoculum Substances 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 235000019738 Limestone Nutrition 0.000 description 1
- 235000013409 condiments Nutrition 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000006028 limestone Substances 0.000 description 1
- 235000020795 whole food diet Nutrition 0.000 description 1
Landscapes
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses an artificial planting method for producing truffles at a high yield. The planting method is implemented by the following steps of: (1) processing lacebark pine seeds; (2) preparing a sowing substrate; (3) sowing and culturing seedlings; (4) preparing a truffle inoculant; (5) preparing an inoculation substrate; (6) inoculating and culturing seedlings; and (7) culturing truffle seedlings. Due to the adoption of the method, the yield per unit of truffles can be increased to 10kg/mu, a harvesting period is entered 8-10 years after planting, an ecological system is kept and maintained, and the recovery and development of forest cover are promoted.
Description
Technical field
The present invention relates to a kind of artificial method for planting that makes the ferfas high yield.
Background technology
The China ferfas is the bright imperial crown in the wholefood, and is not only delicious, and healthy and helpful to the person, its flavor delicate fragrance is good to eat, is one of mushroom of European and American areas happiness food, mainly makes diet condiment, eats or extracts required composition and be used for cosmetics, because quantity is rare, and is expensive.
The China ferfas can promote growing of plant, improves the diseases and insect pests resistance of plant, keeps and the stable ecosystem, promotes recovery and the growth of forest cover.Can be applied in large area deserted mountain, barren hill afforestation, particularly Keshen specially looks grow the soil-geological condition plantation preferably ferfas woods that forms with limestone.Yet, develop at present the ferfas industry in China also relatively backward, make it really become high, the good in economic efficiency industry of added value, the real real value that embodies its economy, ecology, society, also must make great efforts in raising ferfas output, developing china ferfas industry, the unique advantage of (special weather conditions), land productivity (the special geology and geomorphology in Keshen), people and (culture of food bacterium is of long duration) when Yunnan Province's Developing Yunnan ferfas industry has day particularly, be the important directions that mountainous rural area is built, its prospect is inestimable.
The report that does not also have constructed scheme before the applicant.
Summary of the invention
The present invention has overcome deficiency of the prior art, and a kind of artificial method for planting that makes the ferfas high yield is provided.
In order to solve the technical problem of above-mentioned existence, the present invention adopts following technical proposals to be achieved: a kind of artificial method for planting that makes the ferfas high yield, realize through following steps:
(1) gets 6 months Pinus bungeana Seeds of preservation in the 0-4 ℃ of lower refrigerator, under aseptic condition, carry out disinfecting for three times: the H that at first uses concentration 30%
2O
2Soaked with liquid 0.5 hour, clean with aseptic water washing after cleaning; Then be put in the ethanol of concentration 50% and carry out the 10～20min that sterilizes the second time, clean with aseptic water washing; Putting into the aqueous sodium hypochlorite solution of concentration 1.5～5% sterilizes for the third time again; Use at last aseptic water washing 3～5 times; Place culture dish for subsequent use;
(2) get the peat composed of rotten mosses, vermiculite and perlite in mass ratio 3:1:1 in proportion mixing obtain compound, add entry and make mixture moisture content reach 60% to get final product, obtain seedling medium;
(3) with the seed of getting ready in the step (1), be sown into the seedling medium that obtains in the step (2), sowing depth is 1.5cm-2.5cm, grows seedlings;
(4) the ripe ferfas of hanking, after cleaning up with running water, aseptic water washing 3 times is positioned in the agitator and pulverizes, and obtains the ferfas microbial inoculum;
(5) humus soil sieves, and admixes in right amount water, makes water content reach 40%, adds river sand and earth again, mixes with the ratio of mass ratio 4/2/4, obtains inoculating matrix, and is for subsequent use;
(6) step (3) gained seedling is taken out in the matrix of growing seedlings, the root system of ferfas seedling is immersed the ferfas microbial inoculum at least 5 minutes of step (4) preparation, take out the inoculation matrix of implantation step (5) gained and carry out seedling culture, with shading network control light at 40%-60%, and the control temperature was cultivated 3-5 days at 20-26 ℃;
(7) after 3-5 days, the inoculation matrix of implanting seedling is placed the gravity-flow ventilation booth, water annual sunshine duration 1890--2840h.
Owing to adopting technique scheme, can make the per unit area yield of ferfas bring up to 10kg/ mu by 3kg/ mu by the present invention, enter harvest time to 8-10 after the plantation; And ferfas can promote growing of Chinese lace-bark pine, and Chinese lace-bark pine can promote again growing of ferfas to improve the diseases and insect pests resistance of plant simultaneously, keeps and the stable ecosystem, promotes recovery and the growth of forest cover.
Embodiment
The invention will be further described below in conjunction with embodiment:
(1) gets 6 months Pinus bungeana Seeds of preservation in 2 ℃ of refrigerators, under aseptic condition, carry out disinfecting for three times: the H that at first uses concentration 30%
2O
2Soaked with liquid 0.5 hour, clean with aseptic water washing after cleaning; Then be put in the ethanol of concentration 50% and carry out the 10min that sterilizes the second time, clean with aseptic water washing; Putting into the aqueous sodium hypochlorite solution of concentration 3% sterilizes for the third time again; Use at last aseptic water washing 3 times; Place culture dish for subsequent use;
(2) get the peat composed of rotten mosses, vermiculite and perlite in mass ratio 3:1:1 in proportion mixing obtain compound, add entry and make mixture moisture content reach 60% to get final product, obtain seedling medium;
(3) with the seed of getting ready in the step (1), be sown into the seedling medium that obtains in the step (2), sowing depth is 2cm, grows seedlings;
(4) the ripe ferfas of hanking, after cleaning up with running water, aseptic water washing 3 times is positioned in the agitator and pulverizes, and obtains the ferfas microbial inoculum;
(5) humus soil sieves, and admixes in right amount water, makes water content reach 40%, adds river sand and earth again, mixes with the ratio of mass ratio 4/2/4, obtains inoculating matrix, and is for subsequent use;
(6) step (3) gained seedling is taken out in the matrix of growing seedlings, the root system of ferfas seedling is immersed the ferfas microbial inoculum 8 minutes of step (4) preparation, takes out the inoculation matrix of implantation step (5) gained and carry out seedling culture, with shading network control light 50%, and the control temperature was cultivated 5 days at 20 ℃;
After (7) 5 days, the inoculation matrix of implanting seedling is placed the gravity-flow ventilation booth, water annual sunshine duration 2165h.
Entered harvest time, per unit area yield 10kg/ mu on the 8th year after the plantation.
Claims (1)
1. artificial method for planting that makes the ferfas high yield is characterized in that step is as follows:
(1) gets 6 months Pinus bungeana Seeds of preservation in the 0-4 ℃ of lower refrigerator, under aseptic condition, carry out disinfecting for three times: the H that at first uses concentration 30%
2O
2Soaked with liquid 0.5 hour, clean with aseptic water washing after cleaning; Then be put in the ethanol of concentration 50% and carry out the 10～20min that sterilizes the second time, clean with aseptic water washing; Putting into the aqueous sodium hypochlorite solution of concentration 1.5～5% sterilizes for the third time again; Use at last aseptic water washing 3～5 times; Place culture dish for subsequent use;
(2) get the peat composed of rotten mosses, vermiculite and perlite in mass ratio 3:1:1 in proportion mixing obtain compound, add entry and make mixture moisture content reach 60% to get final product, obtain seedling medium;
(3) with the seed of getting ready in the step (1), be sown into the seedling medium that obtains in the step (2), sowing depth is 1.5cm-2.5cm, grows seedlings;
(4) the ripe ferfas of hanking, after cleaning up with running water, aseptic water washing 3 times is positioned in the agitator and pulverizes, and obtains the ferfas microbial inoculum;
(5) humus soil sieves, and admixes in right amount water, makes water content reach 40%, adds river sand and earth again, mixes with the ratio of mass ratio 4/2/4, obtains inoculating matrix, and is for subsequent use;
(6) step (3) gained seedling is taken out in the matrix of growing seedlings, the root system of ferfas seedling is immersed the ferfas microbial inoculum at least 5 minutes of step (4) preparation, take out the inoculation matrix of implantation step (5) gained and carry out seedling culture, with shading network control light at 40%-60%, and the control temperature was cultivated 3-5 days at 20-26 ℃;
(7) after 3-5 days, the inoculation matrix of implanting seedling is placed the gravity-flow ventilation booth, water annual sunshine duration 1890--2840h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210588202.3A CN103026884B (en) | 2012-12-29 | 2012-12-29 | Artificial planting method for producing truffles at high yield |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210588202.3A CN103026884B (en) | 2012-12-29 | 2012-12-29 | Artificial planting method for producing truffles at high yield |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103026884A true CN103026884A (en) | 2013-04-10 |
| CN103026884B CN103026884B (en) | 2014-04-16 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210588202.3A Expired - Fee Related CN103026884B (en) | 2012-12-29 | 2012-12-29 | Artificial planting method for producing truffles at high yield |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103733884A (en) * | 2014-01-13 | 2014-04-23 | 攀枝花市丰盛源农林开发有限公司 | Woodland microenvironment reform propagation promoting method of truffles |
| CN103749154A (en) * | 2014-01-13 | 2014-04-30 | 攀枝花市丰盛源农林开发有限公司 | Culture medium for promoting cross infection among truffle mycorrhizas and using method thereof |
| CN105917913A (en) * | 2016-04-28 | 2016-09-07 | 四川省农业科学院土壤肥料研究所 | Method for breeding mycorrhizal seedlings by truffles and quercus acutissima |
| CN105948889A (en) * | 2016-04-28 | 2016-09-21 | 四川省农业科学院土壤肥料研究所 | Special inoculation composite agent used for artificial cultivation of truffle mycorrhizal seedlings, and preparation method and application method thereof |
| CN106978354A (en) * | 2017-03-11 | 2017-07-25 | 云南绿辰生物科技开发有限公司 | A kind of breeding method of high-purity ferfas strain |
| CN107231940A (en) * | 2017-06-05 | 2017-10-10 | 攀枝花市农林科学研究院 | Ferfas is inoculated with bacterium ball and its preparation and application |
| CN107996285A (en) * | 2017-11-29 | 2018-05-08 | 云南露源野生真菌研究所（有限合伙） | A kind of method of Huashan pine seed seedling-raising inoculation India truffle |
| CN108713448A (en) * | 2018-06-04 | 2018-10-30 | 会东县生产力促进中心 | Restore and set greatly inoculation method in a kind of truffle bacterium pool |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1064780A (en) * | 1990-12-10 | 1992-09-30 | 黄迺欢 | The medium of edible truffle and culture technology |
| CN101061781A (en) * | 2007-04-23 | 2007-10-31 | 攀枝花市农林科学研究院 | Chinese truffle and India truffle mycorrhiza fungi seeding cultivating method |
| CN101328464A (en) * | 2008-05-27 | 2008-12-24 | 中国科学院昆明植物研究所 | Synthetic method of truffle and bacteriorhiza |
-
2012
- 2012-12-29 CN CN201210588202.3A patent/CN103026884B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1064780A (en) * | 1990-12-10 | 1992-09-30 | 黄迺欢 | The medium of edible truffle and culture technology |
| CN101061781A (en) * | 2007-04-23 | 2007-10-31 | 攀枝花市农林科学研究院 | Chinese truffle and India truffle mycorrhiza fungi seeding cultivating method |
| CN101328464A (en) * | 2008-05-27 | 2008-12-24 | 中国科学院昆明植物研究所 | Synthetic method of truffle and bacteriorhiza |
Non-Patent Citations (1)
| Title |
|---|
| 弓明钦 等: "黑孢块菌的苗木菌根化合成效果研究", 《林业科学研究》 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103733884A (en) * | 2014-01-13 | 2014-04-23 | 攀枝花市丰盛源农林开发有限公司 | Woodland microenvironment reform propagation promoting method of truffles |
| CN103749154A (en) * | 2014-01-13 | 2014-04-30 | 攀枝花市丰盛源农林开发有限公司 | Culture medium for promoting cross infection among truffle mycorrhizas and using method thereof |
| CN103733884B (en) * | 2014-01-13 | 2016-07-06 | 攀枝花市丰盛源农林开发有限公司 | The short breeding method of woodland microenvironment transformation of truffles |
| CN103749154B (en) * | 2014-01-13 | 2016-11-16 | 攀枝花市丰盛源农林开发有限公司 | A kind of culture matrix promoting truffle mycorrhiza fungi seeding cross infection and using method thereof |
| CN105917913A (en) * | 2016-04-28 | 2016-09-07 | 四川省农业科学院土壤肥料研究所 | Method for breeding mycorrhizal seedlings by truffles and quercus acutissima |
| CN105948889A (en) * | 2016-04-28 | 2016-09-21 | 四川省农业科学院土壤肥料研究所 | Special inoculation composite agent used for artificial cultivation of truffle mycorrhizal seedlings, and preparation method and application method thereof |
| CN105948889B (en) * | 2016-04-28 | 2019-07-05 | 四川省农业科学院土壤肥料研究所 | Artificial culture truffle Va Mycorrhiza Seedling is inoculated with specific complex agent and its preparation method and application method |
| CN106978354A (en) * | 2017-03-11 | 2017-07-25 | 云南绿辰生物科技开发有限公司 | A kind of breeding method of high-purity ferfas strain |
| CN106978354B (en) * | 2017-03-11 | 2020-06-16 | 云南绿辰生物科技开发有限公司 | Cultivation method of high-purity truffle strain |
| CN107231940A (en) * | 2017-06-05 | 2017-10-10 | 攀枝花市农林科学研究院 | Ferfas is inoculated with bacterium ball and its preparation and application |
| CN107996285A (en) * | 2017-11-29 | 2018-05-08 | 云南露源野生真菌研究所（有限合伙） | A kind of method of Huashan pine seed seedling-raising inoculation India truffle |
| CN108713448A (en) * | 2018-06-04 | 2018-10-30 | 会东县生产力促进中心 | Restore and set greatly inoculation method in a kind of truffle bacterium pool |
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| CN103026884B (en) | 2014-04-16 |
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