CN103988681B - The cultivation method of santal is interplanted in tea place - Google Patents
The cultivation method of santal is interplanted in tea place Download PDFInfo
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Abstract
Interplant a cultivation method for santal in tea place, comprising: the step in standby cave; In each cave, first discharge 5 kilograms during plantation and burn soil, then the santal nutrition pot seedling of the false wormwood artemisia host of band is implanted in cave, water with compound micro-ecological preparation 100 times of dilutions and execute, until false wormwood artemisia host and sandalwood seedling all survive; If there is false wormwood artemisia death to reseed in time, and in time the false wormwood artemisia exceeding santal trunk height is cut off; Annual spring repaiies sprouting bottom santal stem, and prunes santal major branch; During weeding in summer, the plant near santal is retained; In Second Year spring after plantation, survived the root 0.8-1m place of santal in distance, planted a strain U.S. stamen flower as second phase additional host plant, and U.S. stamen stamen or pistil is planted and survives.The present invention makes full use of the space plantation santal in the middle of tea place, reduce the workload of tea place operation intertill and clean tillage, better conserve water and soil, reduce tea place and cave in, and the tealeaves plucked has better fragrance, improves the quality of tealeaves.
Description
Technical field
The present invention relates in a Plants and interplant another kind of cultivation of plants method, particularly relate to the cultivation method of interplanting santal in a kind of tea place.
Background technology
Santal (formal name used at school: SantalumalbumL.) is Santalaceae santal genus, it is a kind of semiparasite aiphyllium, have another name called that bath is fragrant, Santalum album, be mainly distributed in India, Malaysia, Australia, the subtropics of Indonesia and China and torrid areas.The sapwood white of santal trunk, odorlessness, heartwood yellowish-brown, has strong aroma, is valuable medicinal material and famous and precious spices, and is the good timber of artistic carving.
Because santal serves many purposes, economic worth is high, is therefore felled for a long time.At present, santal wild resource is day by day exhausted, and supply falls short of demand for santal, both at home and abroad mainly through adopting the form of artificial planting to make up the demand of market to santal.But because santal growth limit by conditions such as landform, illumination, temperature, soil, therefore produced santal still can not meet the demand in market.
The aiphyllium that tealeaves (formal name used at school Camelliasinensis (L.) O.Kuntze) is Theaceae Camellia, the tealeaves in zhang-ping area is generally dungarunga.The natural conditions of tea tree planting comprise landforms, weather, soil types etc.Landform is based on hills, and drainage condition will be got well, and precipitation is abundant, and annual range of temperature is little, day and night the temperature difference is large, and frost-free season is long, and illumination condition is good, and such weather conditions are suitable for various types of growth of tea plant.Brick red soil development degree is comparatively dark, and well-formed is applicable to growth of tea plant.At Tea planting first 5 years, tea tree was less, and the gap between tea is large, is not fully used in soil, or normal growth of weeds causes fertilizer to be wasted and manual labor increases, or caused water and soil loss due to ground exposure.And tealeaves needs after generally planting 10 years again to plant, the state do not made full use of is got back to again in soil, tea place, and therefore the method for a set of Appropriate application is needed in soil, tea place badly.
Summary of the invention
Technical problem to be solved by this invention is to provide the cultivation method of interplanting santal in a kind of tea place, make full use of the ground, space in the middle of tea place, limited tea place land resources produces larger economic benefit, and makes tealeaves have better fragrance, improve the quality of tealeaves.
The present invention solves the problems of the technologies described above by the following technical programs: the cultivation method of interplanting santal in a kind of tea place, comprises the following steps:
Step 1: select the torrid zone or subtropical zone, annual minimum temperature is not less than-5 DEG C, height above sea level is lower than the tea place, knob of 700 meters, and tea place soil layer is deep, and soil is fertile, loose red soil, and the strain of tea tree, line-spacing are 35cm × 1.5m;
Step 2: dig cave in the ranks tea tree, cave is 50cm deeply, and cave diameter is 35cm, and cave spacing is more than or equal to 3m × 3m, and cave density is every mu and is equal to or less than 20, and the position of planting plant hole must must not affect tea place normal production operation;
Step 3: plant santal during annual spring to autumn, in each cave, first discharge 5 kilograms during plantation and burn soil, then the santal nutrition pot seedling of the false wormwood artemisia host of band is implanted in cave, soil in training, tread, more clear for compound micro-ecological preparation 100 times water-reducible dilution is irrigated as normal root water; Notice that observation survives situation, as soil, dry weather should be watered in time and execute the clear water-reducible dilution of compound micro-ecological preparation 100 times, until false wormwood artemisia host and sandalwood seedling are all survived;
Step 4: if there is false wormwood artemisia death to reseed in time, and in time the false wormwood artemisia exceeding santal trunk height is cut off, and must not water and apply fertilizer after entering the winter every year, winter is the insulation of santal binding hay; Annual spring repaiies sprouting bottom santal stem, and prunes santal major branch, organic fertilizer and spray compound micro-ecological preparation 100 times dilution after dilution; During tea place weeding in summer, the plant near santal is retained;
Step 5: Second Year spring after plantation, survive the root 0.8-1m place of santal in distance, planted a strain U.S. stamen flower as second phase additional host plant, notice that U.S. stamen flower planting location must not affect the normal production operation in tea place, and U.S. stamen stamen or pistil plantation survives, otherwise should reseed in time.
Further, described compound micro-ecological preparation, its preparation method is as follows:
(1) former strain inclined plane is cultivated: the photosynthetic bacteria in freezing pipe, azotobacter, silicate bacterium, blue-green algae, saccharomyces cerevisiae are activated in inclined-plane respectively, be placed in 90mm culture dish afterwards respectively and carry out purifying, obtain photosynthetic bacteria slant strains, azotobacter slant strains, silicate bacterium slant strains, blue-green algae slant strains and saccharomyces cerevisiae slant strains, for subsequent use;
(2) former strain liquid is cultivated: photosynthetic bacteria slant strains be inoculated in sterilized liquid nutrient medium I, 28 DEG C-30 DEG C anaerobism tengsten lamps that are placed within illumination cultivation 5-7 days, obtain photosynthetic bacteria original bacteria liquid; Azotobacter slant strains be inoculated in sterilized liquid nutrient medium II, then at 25 DEG C-27 DEG C, aerobic cultivation obtains azotobacter original bacteria liquid in 1-2 days; Silicate bacterium slant strains be inoculated in sterilized liquid nutrient medium III, then at 28 DEG C-30 DEG C, aerobic cultivation obtains silicate bacterium original bacteria liquid in 1-2 days; Blue-green algae slant strains is inoculated in sterilized liquid nutrient medium IV, is then placed in 28 DEG C-30 DEG C fluorescent lamps and irradiates 10-14 hour and aerobic cultivation 3-5 days every day, obtain blue-green algae original bacteria liquid; Saccharomyces cerevisiae slant strains be inoculated in sterilized liquid nutrient medium V, at 28 DEG C-30 DEG C, aerobic cultivation obtains saccharomyces cerevisiae original bacteria liquid in 3 days afterwards;
(3) first order seed is cultivated: photosynthetic bacteria original bacteria liquid, azotobacter original bacteria liquid, silicate bacterium original bacteria liquid, blue-green algae original bacteria liquid and saccharomyces cerevisiae original bacteria liquid are inoculated in sterilized fermentation medium I by the bacterium amount ratio of 2:2:2:2:1 and cultivate, particularly: first Azotobacter original bacteria liquid, silicate bacterium original bacteria liquid, blue-green algae original bacteria liquid and saccharomyces cerevisiae original bacteria liquid, and in 28 DEG C-30 DEG C aerobic cultivations 3 days, and then inoculate photosynthetic bacteria original bacteria liquid, in 37 DEG C of Anaerobic culturel 3 days, obtain the first order seed of compound micro-ecological preparation;
(4) secondary seed is cultivated: by the first order seed of gained by 5% inoculum concentration be inoculated in sterilized fermentation medium II, and at 28 DEG C-30 DEG C first aerobic cultivation 3-5 days Anaerobic culturel 3-5 days again, obtain the secondary seed of compound micro-ecological preparation;
The component of described fermentation medium II: tangerine water 5%, dusty yeast 0.1%, peptone 0.1%, ammonium chloride 0.2%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, zinc sulphate 0.025%, ferrous sulfate 0.025%, surplus is water;
(5) finished product is cultivated: by the secondary seed of gained by 5% inoculum concentration be seeded in sterilized fermentation medium III, and at 28 DEG C-30 DEG C first aerobic cultivations 3-5 days Anaerobic culturel 3-5 days again, the finished product of acquisition compound micro-ecological preparation;
The component of described fermentation medium III: tangerine water 5%, ammonium chloride 0.2%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, zinc sulphate 0.025%, ferrous sulfate 0.025%, surplus is water.
Further, the concrete operations that in described step (1), photosynthetic bacteria activates in inclined-plane are: be inoculated on sterilized slant medium I with oese by photosynthetic bacteria, afterwards in 28 DEG C-30 DEG C anaerobism tengsten lamp illumination cultivation 5-7 days; The sterilising temp of described slant medium I is 121 DEG C, sterilization time is 15min; And the component of this slant medium I: dusty yeast 10 grams, dipotassium hydrogen phosphate 1 gram, 0.5 gram, magnesium sulfate, 20 grams, agar, water 1000ml, pH7.0-7.2.
Further, the concrete operations that azotobacter activate in inclined-plane in described step (1) are: with oese by inoculating on sterilized slant medium II, aerobic cultivation 1-2 days at 25 DEG C-27 DEG C afterwards; The sterilising temp of described slant medium II is 121 DEG C, sterilization time is 20min; And the component of this slant medium II: dusty yeast 0.5 gram, 20 grams, mannitol, potassium dihydrogen phosphate 0.2 gram, dipotassium hydrogen phosphate 0.8 gram, 0.2 gram, magnesium sulfate, 0.1 gram, calcium sulphate, 15 grams, agar, 0.01 gram, iron chloride, sodium molybdate 0.01 gram, 15 grams, agar, water 1000ml, pH7.2.
Further, the concrete operations that described step (1) mesosilicic acid salt bacterium activates in inclined-plane are: be inoculated on sterilized slant medium III with oese by silicate bacterium, afterwards aerobic cultivation 1-2 days at 25 DEG C-28 DEG C; The sterilising temp of described slant medium III is 121 DEG C, sterilization time is 20min; And the component of this slant medium III: sucrose 10 grams, 5 grams, calcium sulphate, dipotassium hydrogen phosphate 0.2 gram, 5 grams, calcium carbonate, 0.2 gram, magnesium sulfate, 0.2 gram, sodium chloride, 14 grams, agar, water 1000ml, pH7.5.
Further, the concrete operations that in described step (1), blue-green algae activates in inclined-plane are: be inoculated on sterilized slant medium IV with oese by blue-green algae, irradiate 10-14 hour and aerobic cultivation 3-5 days lower every day afterwards in 28 DEG C-30 DEG C fluorescent lamps; The sterilising temp of described slant medium IV is 121 DEG C, sterilization time is 20min; And the component of this slant medium IV: 1.5 grams, sodium nitrate, dipotassium hydrogen phosphate 0.05 gram, 0.08 gram, magnesium sulfate, 0.04 gram, calcium chloride, 0.02 gram, sodium carbonate, citric acid 0.006 gram, ironic citrate 0.006 gram, 0.001 gram, zinc sulphate, manganese chloride 0.001 gram, 20 grams, agar, water 1000ml, PH nature.
Further, the concrete operations that saccharomyces cerevisiae activates in inclined-plane in described step (1) are: be inoculated on sterilized slant medium V with oese by saccharomyces cerevisiae, aerobic cultivation 3 days at 28 DEG C-30 DEG C afterwards; The sterilising temp of described slant medium V is 121 DEG C, sterilization time is 30min; And the component of this slant medium V: potato 200 grams, sucrose 20 grams, agar 15-20 gram, water 1000ml, PH nature.
Further, the component of the middle liquid nutrient medium I of described step (2): dusty yeast 10 grams, dipotassium hydrogen phosphate 1 gram, 0.5 gram, magnesium sulfate, water 1000ml, pH7.0-7.2; The component of liquid nutrient medium II: dusty yeast 0.5 gram, 20 grams, mannitol, potassium dihydrogen phosphate 0.2 gram of dipotassium hydrogen phosphate 0.8 gram, 0.2 gram, magnesium sulfate, 0.1 gram, calcium sulphate, 0.01 gram, iron chloride, sodium molybdate 0.01 gram, water 1000ml, pH7.2; The component of liquid nutrient medium III: sucrose 10 grams, 5 grams, calcium sulphate, dipotassium hydrogen phosphate 0.2 gram, 5 grams, calcium carbonate, 0.2 gram, magnesium sulfate, 0.2 gram, sodium chloride, water 1000ml, pH7.5; The component of liquid nutrient medium IV: 1.5 grams, sodium nitrate, dipotassium hydrogen phosphate 0.05 gram, 0.08 gram, magnesium sulfate, 0.04 gram, calcium chloride, 0.02 gram, sodium carbonate, citric acid 0.006 gram, ironic citrate 0.006 gram, 0.001 gram, zinc sulphate, manganese chloride 0.001 gram, water 1000ml, PH nature; The component of liquid nutrient medium V: potato 200 grams, sucrose 20 grams, water 1000ml, PH nature.
Further, the component of fermentation medium I in described step (3): tangerine water 5%, dusty yeast 0.5%, peptone 1%, ammonium chloride 0.2%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, zinc sulphate 0.025%, ferrous sulfate 0.025%, surplus is water.
Beneficial effect of the present invention is: make full use of the ground, space in the middle of tea place, reduce the workload of intertill and clean tillage, conserve water and soil, reduce tea place embankment, limited tea place land resources produces larger economic benefit; And with the tealeaves that cultivation method of the present invention is plucked, there is better fragrance, improve the quality of tealeaves.
Embodiment
Interplant a cultivation method for santal in tea place, comprise the following steps:
Step 1: select the torrid zone or subtropical zone, annual minimum temperature is not less than-5 DEG C, height above sea level is lower than the tea place, knob of 700 meters, and tea place soil layer is deep, and soil is fertile, loose red soil, and the strain of tea tree, line-spacing are 35cm × 1.5m;
Step 2: dig cave in the ranks tea tree, cave is 50cm deeply, and cave diameter is 35cm, and cave spacing is more than or equal to 3m × 3m, and cave density is every mu and is equal to or less than 20, and the position of planting plant hole must not affect tea place normal production operation;
Step 3: during annual spring to autumn (namely during temperature more than 20 DEG C) plant santal, in each cave, first discharge 5 kilograms during plantation and burn soil, again the santal nutrition pot seedling of the false wormwood artemisia host of band is implanted in cave, soil in training, tread, more clear for compound micro-ecological preparation 100 times water-reducible dilution is irrigated as normal root water; Notice that observation survives situation, as soil, dry weather should be watered in time and execute the clear water-reducible dilution of compound micro-ecological preparation 100 times, until false wormwood artemisia host and sandalwood seedling are all survived;
Step 4: the management carrying out santal and false wormwood artemisia host, if too arid is watered in time, suitable sealing fertilizer or fertilizer, to make false wormwood artemisia and santal robust growth, if there is false wormwood artemisia death to reseed in time, and in time the false wormwood artemisia exceeding santal trunk height is cut off, and must not water and apply fertilizer after entering the winter every year, winter is the insulation of santal binding hay, or the mode such as sootiness prevents frost before cold wave arrives; Annual spring repaiies sprouting bottom santal stem, and prunes to make santal major branch to give prominence to santal major branch, sturdy, organic fertilizer dilution after spraying compound micro-ecological preparation 100 times dilution; During tea place weeding in summer, the plant near santal is retained, namely notes the plant suitably retained near sandalwood seedling when tea place weeding, for limit, field, terraced wall area santal surrounding growth plant only otherwise affect all being retained of growth of tea plant;
Step 5: Second Year spring after plantation, survive root 0.8-1m i.e. about 1 meter of of santal in distance, planted a strain U.S. stamen flower as second phase additional host plant, noticed that plantation U.S. stamen flower position must not affect the normal production operation in tea place, and U.S. stamen stamen or pistil ensures that plantation survives, otherwise reseed in time.
Described compound micro-ecological preparation, its preparation method is as follows:
(1) former strain inclined plane is cultivated: the photosynthetic bacteria in freezing pipe, azotobacter, silicate bacterium, blue-green algae, saccharomyces cerevisiae are activated in inclined-plane respectively, be placed in 90mm culture dish afterwards respectively and carry out purifying, obtain photosynthetic bacteria slant strains, azotobacter slant strains, silicate bacterium slant strains, blue-green algae slant strains and saccharomyces cerevisiae slant strains, for subsequent use;
(2) former strain liquid is cultivated: photosynthetic bacteria slant strains be inoculated in sterilized liquid nutrient medium I, 28 DEG C-30 DEG C anaerobism tengsten lamps that are placed within illumination cultivation 5-7 days, obtain photosynthetic bacteria original bacteria liquid; Azotobacter slant strains be inoculated in sterilized liquid nutrient medium II, then at 25 DEG C-27 DEG C, aerobic cultivation obtains azotobacter original bacteria liquid in 1-2 days; Silicate bacterium slant strains be inoculated in sterilized liquid nutrient medium III, then at 28 DEG C-30 DEG C, aerobic cultivation obtains silicate bacterium original bacteria liquid in 1-2 days; Blue-green algae slant strains is inoculated in sterilized liquid nutrient medium IV, is then placed in 28 DEG C-30 DEG C fluorescent lamps and irradiates 10-14 hour and aerobic cultivation 3-5 days every day, obtain blue-green algae original bacteria liquid; Saccharomyces cerevisiae slant strains be inoculated in sterilized liquid nutrient medium V, at 28 DEG C-30 DEG C, aerobic cultivation obtains saccharomyces cerevisiae original bacteria liquid in 3 days afterwards;
(3) first order seed is cultivated: photosynthetic bacteria original bacteria liquid, azotobacter original bacteria liquid, silicate bacterium original bacteria liquid, blue-green algae original bacteria liquid and saccharomyces cerevisiae original bacteria liquid are inoculated in sterilized fermentation medium I by the bacterium amount ratio of 2:2:2:2:1 and cultivate, particularly: first Azotobacter original bacteria liquid, silicate bacterium original bacteria liquid, blue-green algae original bacteria liquid and saccharomyces cerevisiae original bacteria liquid, and in 28 DEG C-30 DEG C aerobic cultivations 3 days, and then inoculate photosynthetic bacteria original bacteria liquid, in 37 DEG C of Anaerobic culturel 3 days, obtain the first order seed of compound micro-ecological preparation;
(4) secondary seed is cultivated: by the first order seed of gained by 5% inoculum concentration be inoculated in sterilized fermentation medium II, and at 28 DEG C-30 DEG C first aerobic cultivation 3-5 days Anaerobic culturel 3-5 days again, obtain the secondary seed of compound micro-ecological preparation;
(5) finished product is cultivated: by the secondary seed of gained by 5% inoculum concentration be seeded in sterilized fermentation medium III, and at 28 DEG C-30 DEG C first aerobic cultivations 3-5 days Anaerobic culturel 3-5 days again, the finished product of acquisition compound micro-ecological preparation.
Wherein: the concrete operations that in step (1), photosynthetic bacteria activates in inclined-plane are: be inoculated on sterilized slant medium I with oese by photosynthetic bacteria, afterwards in 28 DEG C-30 DEG C anaerobism tengsten lamp illumination cultivation 5-7 days; The sterilising temp of described slant medium I is 121 DEG C, sterilization time is 15min; And the component of this slant medium I: dusty yeast 10 grams, dipotassium hydrogen phosphate 1 gram, 0.5 gram, magnesium sulfate, 20 grams, agar, water 1000ml, pH7.0-7.2.The concrete operations that azotobacter activate in inclined-plane in step (1) are: with oese by inoculating on sterilized slant medium II, aerobic cultivation 1-2 days at 25 DEG C-27 DEG C afterwards; The sterilising temp of described slant medium II is 121 DEG C, sterilization time is 20min; And the component of this slant medium II: dusty yeast 0.5 gram, 20 grams, mannitol, potassium dihydrogen phosphate 0.2 gram, dipotassium hydrogen phosphate 0.8 gram, 0.2 gram, magnesium sulfate, 0.1 gram, calcium sulphate, 15 grams, agar, 0.01 gram, iron chloride, sodium molybdate 0.01 gram, 15 grams, agar, water 1000ml, pH7.2.The concrete operations that step (1) mesosilicic acid salt bacterium activates in inclined-plane are: be inoculated on sterilized slant medium III with oese by silicate bacterium, afterwards aerobic cultivation 1-2 days at 25 DEG C-28 DEG C; The sterilising temp of described slant medium III is 121 DEG C, sterilization time is 20min; And the component of this slant medium III: sucrose 10 grams, 5 grams, calcium sulphate, dipotassium hydrogen phosphate 0.2 gram, 5 grams, calcium carbonate, 0.2 gram, magnesium sulfate, 0.2 gram, sodium chloride, 14 grams, agar, water 1000ml, pH7.5.The concrete operations that in step (1), blue-green algae activates in inclined-plane are: be inoculated on sterilized slant medium IV with oese by blue-green algae, irradiate 10-14 hour and aerobic cultivation 3-5 days lower every day afterwards in 28 DEG C-30 DEG C fluorescent lamps; The sterilising temp of described slant medium IV is 121 DEG C, sterilization time is 20min; And the component of this slant medium IV: 1.5 grams, sodium nitrate, dipotassium hydrogen phosphate 0.05 gram, 0.08 gram, magnesium sulfate, 0.04 gram, calcium chloride, 0.02 gram, sodium carbonate, citric acid 0.006 gram, ironic citrate 0.006 gram, 0.001 gram, zinc sulphate, manganese chloride 0.001 gram, 20 grams, agar, water 1000ml, PH nature.The concrete operations that saccharomyces cerevisiae activates in inclined-plane in step (1) are: be inoculated on sterilized slant medium V with oese by saccharomyces cerevisiae, aerobic cultivation 3 days at 28 DEG C-30 DEG C afterwards; The sterilising temp of described slant medium V is 121 DEG C, sterilization time is 30min; And the component of this slant medium V: potato 200 grams, sucrose 20 grams, agar 15-20 gram, water 1000ml, PH nature.
The component of liquid nutrient medium I in step (2): dusty yeast 10 grams, dipotassium hydrogen phosphate 1 gram, 0.5 gram, magnesium sulfate, water 1000ml, pH7.0-7.2; The component of liquid nutrient medium II: dusty yeast 0.5 gram, 20 grams, mannitol, potassium dihydrogen phosphate 0.2 gram of dipotassium hydrogen phosphate 0.8 gram, 0.2 gram, magnesium sulfate, 0.1 gram, calcium sulphate, 0.01 gram, iron chloride, sodium molybdate 0.01 gram, water 1000ml, pH7.2; The component of liquid nutrient medium III: sucrose 10 grams, 5 grams, calcium sulphate, dipotassium hydrogen phosphate 0.2 gram, 5 grams, calcium carbonate, 0.2 gram, magnesium sulfate, 0.2 gram, sodium chloride, water 1000ml, pH7.5; The component of liquid nutrient medium IV: 1.5 grams, sodium nitrate, dipotassium hydrogen phosphate 0.05 gram, 0.08 gram, magnesium sulfate, 0.04 gram, calcium chloride, 0.02 gram, sodium carbonate, citric acid 0.006 gram, ironic citrate 0.006 gram, 0.001 gram, zinc sulphate, manganese chloride 0.001 gram, water 1000ml, PH nature; The component of liquid nutrient medium V: potato 200 grams, sucrose 20 grams, water 1000ml, PH nature.
The component of fermentation medium I in step (3): tangerine water 5%, dusty yeast 0.5%, peptone 1%, ammonium chloride 0.2%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, zinc sulphate 0.025%, ferrous sulfate 0.025%, surplus is water.The component of fermentation medium II in step (4): tangerine water 5%, dusty yeast 0.1%, peptone 0.1%, ammonium chloride 0.2%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, zinc sulphate 0.025%, ferrous sulfate 0.025%, surplus is water.The component of fermentation medium III in step (5): tangerine water 5%, ammonium chloride 0.2%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, zinc sulphate 0.025%, ferrous sulfate 0.025%, surplus is water.
It should be noted that, in the present invention, the percentage of each medium is mass percent.
Table 1 for interplant the affect situation of santal on tealeaves soluble sugar, chlorophyll, proline content in tea place, table 2 for interplanting the affect situation of santal on tealeaves polyphenol content and output in tea place, the annual value of production of the santal that table 3 is interplanted for tea place, specifically as shown in each table:
The impact of santal on tealeaves soluble sugar, chlorophyll, proline content interplanted by table 1
The impact of santal on tealeaves polyphenol content and output interplanted by table 2
Note: polyphenol content measures and adopts GB/T8313-2002
The santal annual value of production of table 3 tea place interplanting
| Quantity (plant/acre) | Unit price (unit/strain) | Planting Years (year) | Annual value of production (unit) |
| 20 | 15000 | 10 | 30000 |
Adopt the present invention can realize the ground, space made full use of in the middle of tea tree, reduce the workload of intertill and clean tillage, conserve water and soil, reduce tea place embankment, limited tea place land resources produces larger economic benefit; And with the tealeaves that this cultivation method is plucked, there is better fragrance, improve the quality of tealeaves.
Claims (9)
1. interplant a cultivation method for santal in tea place, it is characterized in that: comprise the following steps:
Step 1: select the torrid zone or subtropical zone, annual minimum temperature is not less than-5 DEG C, height above sea level is lower than the tea place, knob of 700 meters, and tea place soil layer is deep, and soil is fertile, loose red soil, and the strain of tea tree, line-spacing are 35cm × 1.5m;
Step 2: dig cave in the ranks tea tree, cave is 50cm deeply, and cave diameter is 35cm, and cave spacing is more than or equal to 3m × 3m, and cave density is every mu and is equal to or less than 20;
Step 3: plant santal during annual spring to autumn, in each cave, first discharge 5 kilograms during plantation and burn soil, then the santal nutrition pot seedling of the false wormwood artemisia host of band is implanted in cave, soil in training, tread, more clear for compound micro-ecological preparation 100 times water-reducible dilution is irrigated as normal root water; If soil, dry weather should be watered in time execute the clear water-reducible dilution of compound micro-ecological preparation 100 times, until false wormwood artemisia host and sandalwood seedling all survive;
Step 4: if there is false wormwood artemisia death to reseed in time, and in time the false wormwood artemisia exceeding santal trunk height is cut off, and must not water and apply fertilizer after entering the winter every year, winter is the insulation of santal binding hay; Annual spring prunes sprouting bottom santal stem, and prunes santal major branch, execute a fertilizer and spray compound micro-ecological preparation 100 times dilution after dilution; During tea place weeding in summer, the plant near santal is retained;
Step 5: Second Year spring after plantation, has survived the root 0.8-1m place of santal in distance, plant the U.S. stamen flower of a strain and add host plant as the second phase, and U.S. stamen stamen or pistil ensures that plantation survives, otherwise should reseed in time.
2. interplant the cultivation method of santal in tea place as claimed in claim 1, it is characterized in that:
Described compound micro-ecological preparation, its preparation method is as follows:
(1) former strain inclined plane is cultivated: the photosynthetic bacteria in freezing pipe, azotobacter, silicate bacterium, blue-green algae, saccharomyces cerevisiae are activated in inclined-plane respectively, be placed in 90mm culture dish afterwards respectively and carry out purifying, obtain photosynthetic bacteria slant strains, azotobacter slant strains, silicate bacterium slant strains, blue-green algae slant strains and saccharomyces cerevisiae slant strains, for subsequent use;
(2) former strain liquid is cultivated: photosynthetic bacteria slant strains be inoculated in sterilized liquid nutrient medium I, 28 DEG C-30 DEG C anaerobism tengsten lamps that are placed within illumination cultivation 5-7 days, obtain photosynthetic bacteria original bacteria liquid; Azotobacter slant strains be inoculated in sterilized liquid nutrient medium II, then at 25 DEG C-27 DEG C, aerobic cultivation obtains azotobacter original bacteria liquid in 1-2 days; Silicate bacterium slant strains be inoculated in sterilized liquid nutrient medium III, then at 28 DEG C-30 DEG C, aerobic cultivation obtains silicate bacterium original bacteria liquid in 1-2 days; Blue-green algae slant strains is inoculated in sterilized liquid nutrient medium IV, is then placed in 28 DEG C-30 DEG C fluorescent lamps and irradiates 10-14 hour and aerobic cultivation 3-5 days every day, obtain blue-green algae original bacteria liquid; Saccharomyces cerevisiae slant strains be inoculated in sterilized liquid nutrient medium V, at 28 DEG C-30 DEG C, aerobic cultivation obtains saccharomyces cerevisiae original bacteria liquid in 3 days afterwards;
(3) first order seed is cultivated: photosynthetic bacteria original bacteria liquid, azotobacter original bacteria liquid, silicate bacterium original bacteria liquid, blue-green algae original bacteria liquid and saccharomyces cerevisiae original bacteria liquid are inoculated in sterilized fermentation medium I by the bacterium amount ratio of 2:2:2:2:1 and cultivate, particularly: first Azotobacter original bacteria liquid, silicate bacterium original bacteria liquid, blue-green algae original bacteria liquid and saccharomyces cerevisiae original bacteria liquid, and in 28 DEG C-30 DEG C aerobic cultivations 3 days, and then inoculate photosynthetic bacteria original bacteria liquid, in 37 DEG C of Anaerobic culturel 3 days, obtain the first order seed of compound micro-ecological preparation;
(4) secondary seed is cultivated: by the first order seed of gained by 5% inoculum concentration be inoculated in sterilized fermentation medium II, and at 28 DEG C-30 DEG C first aerobic cultivation 3-5 days Anaerobic culturel 3-5 days again, obtain the secondary seed of compound micro-ecological preparation;
The component of described fermentation medium II: tangerine water 5%, dusty yeast 0.1%, peptone 0.1%, ammonium chloride 0.2%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, zinc sulphate 0.025%, ferrous sulfate 0.025%, surplus is water;
(5) finished product is cultivated: by the secondary seed of gained by 5% inoculum concentration be seeded in sterilized fermentation medium III, and at 28 DEG C-30 DEG C first aerobic cultivations 3-5 days Anaerobic culturel 3-5 days again, the finished product of acquisition compound micro-ecological preparation;
The component of described fermentation medium III: tangerine water 5%, ammonium chloride 0.2%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, zinc sulphate 0.025%, ferrous sulfate 0.025%, surplus is water.
3. in tea place according to claim 2, interplant the cultivation method of santal, it is characterized in that: the concrete operations that in described step (1), photosynthetic bacteria activates in inclined-plane are: be inoculated on sterilized slant medium I with oese by photosynthetic bacteria, afterwards in 28 DEG C-30 DEG C anaerobism tengsten lamp illumination cultivation 5-7 days; The sterilising temp of described slant medium I is 121 DEG C, sterilization time is 15min; And the component of this slant medium I: dusty yeast 10 grams, dipotassium hydrogen phosphate 1 gram, 0.5 gram, magnesium sulfate, 20 grams, agar, water 1000ml, pH7.0-7.2.
4. in tea place according to claim 2, interplant the cultivation method of santal, it is characterized in that: the concrete operations that azotobacter activate in inclined-plane in described step (1) are: with oese by inoculating on sterilized slant medium II, aerobic cultivation 1-2 days at 25 DEG C-27 DEG C afterwards; The sterilising temp of described slant medium II is 121 DEG C, sterilization time is 20min; And the component of this slant medium II: dusty yeast 0.5 gram, 20 grams, mannitol, potassium dihydrogen phosphate 0.2 gram, dipotassium hydrogen phosphate 0.8 gram, 0.2 gram, magnesium sulfate, 0.1 gram, calcium sulphate, 15 grams, agar, 0.01 gram, iron chloride, sodium molybdate 0.01 gram, 15 grams, agar, water 1000ml, pH7.2.
5. in tea place according to claim 2, interplant the cultivation method of santal, it is characterized in that: the concrete operations that described step (1) mesosilicic acid salt bacterium activates in inclined-plane are: with oese, silicate bacterium is inoculated on sterilized slant medium III, afterwards aerobic cultivation 1-2 days at 25 DEG C-28 DEG C; The sterilising temp of described slant medium III is 121 DEG C, sterilization time is 20min; And the component of this slant medium III: sucrose 10 grams, 5 grams, calcium sulphate, dipotassium hydrogen phosphate 0.2 gram, 5 grams, calcium carbonate, 0.2 gram, magnesium sulfate, 0.2 gram, sodium chloride, 14 grams, agar, water 1000ml, pH7.5.
6. in tea place according to claim 2, interplant the cultivation method of santal, it is characterized in that: the concrete operations that in described step (1), blue-green algae activates in inclined-plane are: be inoculated on sterilized slant medium IV with oese by blue-green algae, irradiate 10-14 hour and aerobic cultivation 3-5 days lower every day afterwards in 28 DEG C-30 DEG C fluorescent lamps; The sterilising temp of described slant medium IV is 121 DEG C, sterilization time is 20min; And the component of this slant medium IV: 1.5 grams, sodium nitrate, dipotassium hydrogen phosphate 0.05 gram, 0.08 gram, magnesium sulfate, 0.04 gram, calcium chloride, 0.02 gram, sodium carbonate, citric acid 0.006 gram, ironic citrate 0.006 gram, 0.001 gram, zinc sulphate, manganese chloride 0.001 gram, 20 grams, agar, water 1000ml, PH nature.
7. in tea place according to claim 2, interplant the cultivation method of santal, it is characterized in that: the concrete operations that saccharomyces cerevisiae activates in inclined-plane in described step (1) are: be inoculated on sterilized slant medium V with oese by saccharomyces cerevisiae, aerobic cultivation 3 days at 28 DEG C-30 DEG C afterwards; The sterilising temp of described slant medium V is 121 DEG C, sterilization time is 30min; And the component of this slant medium V: potato 200 grams, sucrose 20 grams, agar 15-20 gram, water 1000ml, PH nature.
8. interplant the cultivation method of santal in tea place according to claim 2, it is characterized in that: the component of liquid nutrient medium I in described step (2): dusty yeast 10 grams, dipotassium hydrogen phosphate 1 gram, 0.5 gram, magnesium sulfate, water 1000ml, pH7.0-7.2; The component of liquid nutrient medium II: dusty yeast 0.5 gram, 20 grams, mannitol, potassium dihydrogen phosphate 0.2 gram of dipotassium hydrogen phosphate 0.8 gram, 0.2 gram, magnesium sulfate, 0.1 gram, calcium sulphate, 0.01 gram, iron chloride, sodium molybdate 0.01 gram, water 1000ml, pH7.2; The component of liquid nutrient medium III: sucrose 10 grams, 5 grams, calcium sulphate, dipotassium hydrogen phosphate 0.2 gram, 5 grams, calcium carbonate, 0.2 gram, magnesium sulfate, 0.2 gram, sodium chloride, water 1000ml, pH7.5; The component of liquid nutrient medium IV: 1.5 grams, sodium nitrate, dipotassium hydrogen phosphate 0.05 gram, 0.08 gram, magnesium sulfate, 0.04 gram, calcium chloride, 0.02 gram, sodium carbonate, citric acid 0.006 gram, ironic citrate 0.006 gram, 0.001 gram, zinc sulphate, manganese chloride 0.001 gram, water 1000ml, PH nature; The component of liquid nutrient medium V: potato 200 grams, sucrose 20 grams, water 1000ml, PH nature.
9. in tea place according to claim 2, interplant the cultivation method of santal, it is characterized in that: the component of fermentation medium I in described step (3): tangerine water 5%, dusty yeast 0.5%, peptone 1%, ammonium chloride 0.2%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, zinc sulphate 0.025%, ferrous sulfate 0.025%, surplus is water.
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| CN103667152A (en) * | 2013-12-19 | 2014-03-26 | 云南晋企生物科技有限公司 | Universal microorganism bacterial agent |
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| CN104521658A (en) * | 2014-11-30 | 2015-04-22 | 刘长华 | Cedar and tea tree interplanting method |
| CN104542164B (en) * | 2015-01-27 | 2016-11-30 | 安徽兰兮工程技术开发有限公司 | A kind of interplanting method of Folium Camelliae sinensis |
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| CN104838955B (en) * | 2015-06-05 | 2017-10-03 | 漳州光照人茶业有限公司 | A kind of tea place interplanting method |
| CN106701608A (en) * | 2015-08-03 | 2017-05-24 | 佛山市绿葵环保科技有限公司 | Microecological modulator |
| CN105646015A (en) * | 2015-12-10 | 2016-06-08 | 中国农业大学 | Composite microelement fertilizer containing chlorella and paenibacillus, preparation and applications thereof |
| CN106332724A (en) * | 2016-08-26 | 2017-01-18 | 李军 | Planting technology and planting methods for early bearing heartwood of algum |
| CN106472575A (en) * | 2016-08-31 | 2017-03-08 | 江苏星源生物科技有限公司 | A kind of preparation method of nursery stock plant growth regulator |
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