CN103026884B - Artificial planting method for producing truffles at high yield - Google Patents
Artificial planting method for producing truffles at high yield Download PDFInfo
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- CN103026884B CN103026884B CN201210588202.3A CN201210588202A CN103026884B CN 103026884 B CN103026884 B CN 103026884B CN 201210588202 A CN201210588202 A CN 201210588202A CN 103026884 B CN103026884 B CN 103026884B
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Abstract
The invention discloses an artificial planting method for producing truffles at a high yield. The planting method is implemented by the following steps of: (1) processing lacebark pine seeds; (2) preparing a sowing substrate; (3) sowing and culturing seedlings; (4) preparing a truffle inoculant; (5) preparing an inoculation substrate; (6) inoculating and culturing seedlings; and (7) culturing truffle seedlings. Due to the adoption of the method, the yield per unit of truffles can be increased to 10kg/mu, a harvesting period is entered 8-10 years after planting, an ecological system is kept and maintained, and the recovery and development of forest cover are promoted.
Description
Technical field
The present invention relates to a kind of artificial method for planting that makes ferfas high yield.
Background technology
China ferfas is the bright imperial crown in wholefood, not only delicious, and healthy and helpful to the person, its taste delicate fragrance is good to eat, is one of mushroom of European and American areas happiness food, mainly makes diet condiment, eats or extract required composition for cosmetics, due to quantity rareness, expensive.
China ferfas can promote growing of plant, improves the diseases and insect pests resistance of plant, keeps and the stable ecosystem, promotes recovery and the growth of forest cover.Can be applied to deserted mountain, barren hill afforestation, the particularly Keshen soil-geological condition plantation preferably ferfas woods that specially looks form with limestone growth in large area.But, in China, develop at present ferfas industry also relatively backward, make it really become high, the good in economic efficiency industry of added value, the real real value that embodies its economy, ecology, society, also must in raising ferfas output, make great efforts, developing china ferfas industry, the particularly unique advantage of (special weather conditions), land productivity (the special geology and geomorphology in Keshen), people and (culture of food bacterium is long-standing) when Yunnan Province's Developing Yunnan ferfas industry has day, be the important directions that mountainous rural area is built, its prospect is inestimable.
Before applicant, also there is no the report of constructed scheme.
Summary of the invention
The present invention has overcome deficiency of the prior art, and a kind of artificial method for planting that makes ferfas high yield is provided.
In order to solve the technical problem of above-mentioned existence, the present invention adopts following technical proposals to be achieved: a kind of artificial method for planting that makes ferfas high yield, through following steps, realize:
(1) get the interior preservation Pinus bungeana Seeds of 6 months of refrigerator at 0-4 ℃, under aseptic condition, carry out disinfecting for three times: the H that first uses concentration 30%
2o
2soaked with liquid 0.5 hour, clean with aseptic water washing after scouring; Then be put into the 10～20min that sterilizes for the second time in the ethanol of concentration 50%, clean with aseptic water washing; Putting into the aqueous sodium hypochlorite solution of concentration 1.5～5% sterilizes for the third time again; Finally use aseptic water washing 3～5 times; Be placed in culture dish standby;
(2) get the peat composed of rotten mosses, vermiculite and perlite in mass ratio 3:1:1 mix in proportion and obtain compound, add water to make mixture moisture content reach 60%, obtain seedling medium;
(3) by the seed of getting ready in step (1), be sown into the seedling medium obtaining in step (2), sowing depth is 1.5cm-2.5cm, grows seedlings;
(4) the ripe ferfas of hanking, after cleaning up with running water, aseptic water washing 3 times, is positioned in agitator and pulverizes, and obtains ferfas microbial inoculum;
(5) humus soil sieves, and admixes in right amount water, makes water content reach 40%, then adds river sand and earth, with the ratio of mass ratio 4/2/4, mixes, and obtains inoculating matrix, standby;
(6) step (3) gained seedling is taken out in seedling medium, ferfas microbial inoculum prepared by the root system immersion step (4) of ferfas seedling at least 5 minutes, take out the inoculation matrix of implantation step (5) gained and carry out seedling culture, with shading network control light at 40%-60%, and control temperature at 20-26 ℃, cultivate 3-5 days;
(7) after 3-5 days, the inoculation matrix of implanting seedling is placed in to gravity-flow ventilation booth, waters, annual sunshine duration 1890--2840h.
Owing to adopting technique scheme, by the present invention, can make the per unit area yield of ferfas bring up to 10kg/ mu by 3kg/ mu, after plantation, to 8-10, enter harvest time; And ferfas can promote growing of Chinese lace-bark pine, Chinese lace-bark pine can promote again growing of ferfas to improve the diseases and insect pests resistance of plant simultaneously, keeps and the stable ecosystem, promotes recovery and the growth of forest cover.
Embodiment
Below in conjunction with embodiment, the invention will be further described:
(1) get the preservation Pinus bungeana Seeds of 6 months in 2 ℃ of refrigerators, under aseptic condition, carry out disinfecting for three times: the H that first uses concentration 30%
2o
2soaked with liquid 0.5 hour, clean with aseptic water washing after scouring; Then be put into the 10min that sterilizes for the second time in the ethanol of concentration 50%, clean with aseptic water washing; Putting into the aqueous sodium hypochlorite solution of concentration 3% sterilizes for the third time again; Finally use aseptic water washing 3 times; Be placed in culture dish standby;
(2) get the peat composed of rotten mosses, vermiculite and perlite in mass ratio 3:1:1 mix in proportion and obtain compound, add water to make mixture moisture content reach 60%, obtain seedling medium;
(3) by the seed of getting ready in step (1), be sown into the seedling medium obtaining in step (2), sowing depth is 2cm, grows seedlings;
(4) the ripe ferfas of hanking, after cleaning up with running water, aseptic water washing 3 times, is positioned in agitator and pulverizes, and obtains ferfas microbial inoculum;
(5) humus soil sieves, and admixes in right amount water, makes water content reach 40%, then adds river sand and earth, with the ratio of mass ratio 4/2/4, mixes, and obtains inoculating matrix, standby;
(6) step (3) gained seedling is taken out in seedling medium, ferfas microbial inoculum prepared by the root system immersion step (4) of ferfas seedling 8 minutes, takes out the inoculation matrix of implantation step (5) gained and carries out seedling culture, uses shading network control light 50%, and control temperature at 20 ℃, cultivate 5 days;
After (7) 5 days, the inoculation matrix of implanting seedling is placed in to gravity-flow ventilation booth, waters, annual sunshine duration 2165h.
From plantation within the 8th year, enter harvest time, per unit area yield 10kg/ mu.
Claims (1)
1. make an artificial method for planting for ferfas high yield, it is characterized in that step is as follows:
(1) get the interior preservation Pinus bungeana Seeds of 6 months of refrigerator at 0-4 ℃, under aseptic condition, carry out disinfecting for three times: the H that first uses concentration 30%
2o
2soaked with liquid 0.5 hour, clean with aseptic water washing after scouring; Then be put into the 10～20min that sterilizes for the second time in the ethanol of concentration 50%, clean with aseptic water washing; Putting into the aqueous sodium hypochlorite solution of concentration 1.5～5% sterilizes for the third time again; Finally use aseptic water washing 3～5 times; Be placed in culture dish standby;
(2) get the peat composed of rotten mosses, vermiculite and perlite in mass ratio 3:1:1 mix in proportion and obtain compound, add water to make mixture moisture content reach 60%, obtain seedling medium;
(3) by the seed of getting ready in step (1), be sown into the seedling medium obtaining in step (2), sowing depth is 1.5cm-2.5cm, grows seedlings;
(4) the ripe ferfas of hanking, after cleaning up with running water, aseptic water washing 3 times, is positioned in agitator and pulverizes, and obtains ferfas microbial inoculum;
(5) humus soil sieves, and admixes in right amount water, makes water content reach 40%, then adds river sand and earth, with the ratio of mass ratio 4/2/4, mixes, and obtains inoculating matrix, standby;
(6) step (3) gained seedling is taken out in seedling medium, ferfas microbial inoculum prepared by the root system immersion step (4) of ferfas seedling at least 5 minutes, take out the inoculation matrix of implantation step (5) gained and carry out seedling culture, with shading network control light at 40%-60%, and control temperature at 20-26 ℃, cultivate 3-5 days;
(7) after 3-5 days, the inoculation matrix of implanting seedling is placed in to gravity-flow ventilation booth, waters, annual sunshine duration 1890--2840h.
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| CN201210588202.3A CN103026884B (en) | 2012-12-29 | 2012-12-29 | Artificial planting method for producing truffles at high yield |
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| CN201210588202.3A CN103026884B (en) | 2012-12-29 | 2012-12-29 | Artificial planting method for producing truffles at high yield |
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| CN103026884B true CN103026884B (en) | 2014-04-16 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103733884B (en) * | 2014-01-13 | 2016-07-06 | 攀枝花市丰盛源农林开发有限公司 | The short breeding method of woodland microenvironment transformation of truffles |
| CN103749154B (en) * | 2014-01-13 | 2016-11-16 | 攀枝花市丰盛源农林开发有限公司 | A kind of culture matrix promoting truffle mycorrhiza fungi seeding cross infection and using method thereof |
| CN105948889B (en) * | 2016-04-28 | 2019-07-05 | 四川省农业科学院土壤肥料研究所 | Artificial culture truffle Va Mycorrhiza Seedling is inoculated with specific complex agent and its preparation method and application method |
| CN105917913A (en) * | 2016-04-28 | 2016-09-07 | 四川省农业科学院土壤肥料研究所 | Method for breeding mycorrhizal seedlings by truffles and quercus acutissima |
| CN106978354B (en) * | 2017-03-11 | 2020-06-16 | 云南绿辰生物科技开发有限公司 | Cultivation method of high-purity truffle strain |
| CN107231940B (en) * | 2017-06-05 | 2020-05-05 | 攀枝花市农林科学研究院 | Truffle inoculation pill and preparation and use method thereof |
| CN107996285A (en) * | 2017-11-29 | 2018-05-08 | 云南露源野生真菌研究所（有限合伙） | A kind of method of Huashan pine seed seedling-raising inoculation India truffle |
| CN108713448A (en) * | 2018-06-04 | 2018-10-30 | 会东县生产力促进中心 | Restore and set greatly inoculation method in a kind of truffle bacterium pool |
Citations (3)
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| CN1064780A (en) * | 1990-12-10 | 1992-09-30 | 黄迺欢 | The medium of edible truffle and culture technology |
| CN101061781A (en) * | 2007-04-23 | 2007-10-31 | 攀枝花市农林科学研究院 | Chinese truffle and India truffle mycorrhiza fungi seeding cultivating method |
| CN101328464A (en) * | 2008-05-27 | 2008-12-24 | 中国科学院昆明植物研究所 | Synthetic method of truffle and bacteriorhiza |
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Patent Citations (3)
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| CN1064780A (en) * | 1990-12-10 | 1992-09-30 | 黄迺欢 | The medium of edible truffle and culture technology |
| CN101061781A (en) * | 2007-04-23 | 2007-10-31 | 攀枝花市农林科学研究院 | Chinese truffle and India truffle mycorrhiza fungi seeding cultivating method |
| CN101328464A (en) * | 2008-05-27 | 2008-12-24 | 中国科学院昆明植物研究所 | Synthetic method of truffle and bacteriorhiza |
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| 弓明钦 等.黑孢块菌的苗木菌根化合成效果研究.《林业科学研究》.2003,第16卷(第1期), |
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