CN104770177B - A kind of Rosa roxburghii isolated shoot method for culturing seedlings and the application in Rocky Desertification Control - Google Patents
A kind of Rosa roxburghii isolated shoot method for culturing seedlings and the application in Rocky Desertification Control Download PDFInfo
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Abstract
It can be used for (Rosa roxburghii) the isolated shoot nursery of quick, easy, efficient Rosa roxburghii and applied to the afforestation technology in Rocky Desertification Control present invention relates particularly to one kind, belong to fruit tree biological technical field.Simple and easy to do, cost of the invention is low, without soil pollution, efficiently, quickly bred suitable for Rosa roxburghii and applied to production practices such as Rocky Desertification Controls, transplanting survival rate is up to more than 95%, had a significant effect using Rocky Desertification Control, administer area's vegetation coverage and improve 6~8%, soil erosion modulus reduction by 15~20%, stony desertification area ratio reduction by 10~15%, so the present invention can not only produce obvious economic benefit, while also having the good ecological benefits such as water and soil conservation, revegetation.
Description
Technical field
The invention belongs to fruit tree biological technical field, and in particular to one kind can be used for quick, easy, efficient Rosa roxburghii
(Rosa roxburghii) isolated shoot method for culturing seedlings and applied to the afforestation technology in Rocky Desertification Control.
Background technology
Rosa roxburghii (Rosa roxbunghii) is rosaceous plant, and wild undershrub is born in facing south for 500-2500 meters of height above sea level
It is the natural wild fruit on the ground such as Guizhou, Mountain Area of Western Hubei Province, the Liangshan Mountain, Mianning mountain area in hillside, cheuch, roadside and shrubbery.Fruit is a kind of
The rare precious fruit of the nutrition that there is healthcare to act on.The harvest time of Rosa roxburghii was less than 30 days.Perverse pears be April to June open pink,
Red or wine-colored flower, the summer flower autumn is real.Fruit is generally oblate spherical, and transverse diameter is generally 2-4 centimetres, August to September fruit into
It is ripe, yellow, sometimes with blush.Pulp is crisp, has dense fragranced after maturation.The close small corn of life on pericarp, custom is referred to as " Rosa roxburghii ".Really
Containing abundant vitamin C in real, it is referred to as " king of Vc ".500-1000 milligrams of tannin；Containing sour 1 gram or so；Total sugar amount is general
4 grams or so.
Karst (Karst) is karst, is water to solublerock, and such as carbonate rock, gypsum, rock salt are carried out with chemistry
Based on corrosion, the general name of the phenomenon produced by geologic process supplemented by the mechanism such as the erosion of flowing water, latent erosion and avalanche.
The landforms as caused by karsitification, claim karst landform, are also karst features.
Stony desertification (Rocky Desertification) refers in the torrid zone, subtropical zone moistening, grassland climate condition and rock
Under the molten natural background extremely developed, disturbed by human activity, surface vegetation is wrecked, cause soil to be seriously lost in, base
Rock large area is exposed or land deterioration phenomenon of gravel buildup, is also the extreme form of karst area land deterioration.
Rosa roxburghii (Rosa roxbunghii Tratt) is the perennial machaka of the rose family.Rosa roxburghii is the Yunnan-Guizhou Plateau and climbed
The western distinctive wild resource in plateau, by main product of Guizhou, Yunnan take second place, the river southeast, North-west Guangxi, the western Hunan, South-West Hubei and Shan Nan are equal
It is distributed.The medical value of Rosa roxburghii is very high, and it is spent, leaf fruit and seed can be used as medicine, and has functions that stomach invigorating, helps digestion, nourishes and antidiarrheal.
Particularly Rosa roxburghii is rich in hepatocuprein (abbreviation S0D), also with antiviral, radiation-resistant effect, in cardiovascular, digestion
In terms of system and various tumor disease preventing and treatings, using quite varied.The fruit of Rosa roxburghii is also simultaneously the first-class of processing health food
Raw material, fruit plants the micro member beneficial to human body rich in sugar, vitamin, carrotene, organic acid and 20 several amino acids, more than 10
Element.Especially Vitamin C content is high, is highest in current fruit, content 841.58~3541.13 in every 100 grams of fresh fruits
Milligram, the laudatory title with " king of vitamin C ".
Rosa roxburghii is resistance very strong species simultaneously, can be grown very well in Karst Rocky Desertification adverse circumstances.It can be seen that,
Research and development Rosa roxburghii nursery can not only develop Rosa roxburghii cultivation and processing industry with stony desertification environment planting technology, be provided for consumer
The health food of high-quality, while the living condition to improvement Karst Rocky Desertification ecological environment, the vast mountain area masses of raising has
Significance.However, so far, the nursery of Rosa roxburghii and stony desertification environment planting technology demand are very urgent, especially Rosa roxburghii
Afforestation technology in Rocky Desertification Control is essentially blank.
Cuttage technique：Cuttage is also referred to as cutting, is a kind of conventional propagation method for cultivating plant.Can with the stem of clip plant,
Leaf, root, bud etc. (claim cuttings) on gardening, or in insertion soil, Sha Zhong, or be immersed in water, can plant, make after taking root
Turn into independent new plant.In agriculture and forestry production, there are different demands to condition during different plant cuttages.Understand and comply with it
Demand, higher breeding success rate could be obtained.
Stock growing technology：Stock growing technology refers to get during seedling goes out the whole cultivating processes in garden from propagating materials
Involved every technical measures, including selection of seedling, site preparation, dig cave, fertilising, earthing, multiple links such as water, manage and protect.
Fast propagation method of seedless roxburgh rose seedlings, including axillary bud deriving, tissue culture seedling rooting, rooted seedling transplanting and other steps,
Specifically：(1) Primary culture of axillary bud deriving, choose excellent seedless roxburgh rose gives birth to tender stem then, and tender stem is first rushed with flowing water
Wash after 10-15 minutes, the stem section for being cut into 5cm or so carries out disinfection, sterilization uses 75% alcohol and 0.1% mercuric chloride combined sterilizing,
Alcohol 30 seconds, mercuric chloride 8~12 minutes uses aseptic water washing 3-4 times after sterilization, the water in stem section is blotted with the filter paper sterilized
Point, then the stem section of 1 axil of band is cut into, oblique cutting is in axillary bud primary culture medium, and axil is upward, it is ensured that can be obtained at axil
To sufficient illumination, primary culture medium is MS+6-BA0.5-2.0mg/L+IBA0.1-0.3mg/L, after 10 days at axil position
Induce axillary bud, continue to cultivate, the condition of whole Primary culture is light intensity 1000-2000Lux, hour/day of light application time 12,
Temperature is between 23-26 DEG C；(2) proliferation-inducing of axillary bud, when axillary bud grows tall into more than 4 centimetres of small after seedling, is cut into 2 centimetres
Long stem section, 1-3 axil of every section of band, then oblique cutting is on shoot proliferation culture medium, and axil is upward, it is ensured that can be obtained at axil
To sufficient illumination, proliferated culture medium is MS+ZT 1.0-1.5mg/L+IBA0.05-0.1mg/L, after 15 days, induces axillary bud,
1-3 adventitious bud seedling can be induced in axillary bud base portion simultaneously, then by the culture of 30 days, seedling can grow to it is 4 centimetres high, afterwards
Seedling is cut into stem section of 2. centimeter lengths with 1-3 axil again to go on identical shoot proliferation culture medium, by the training of 45 days
Support, the seedling derived can grow tall to 4-5 centimetres again, high 4-5 centimetres of seedling is cut into 2 centimeter lengths 1-3 leaf of band repeatedly
The stem section of armpit carries out Multiplying culture on proliferated culture medium, and its proliferation times is 3-4 times, and the condition of whole shoot proliferation culture is equal
For light intensity 1000-2000Lux, hour/day of light application time 12, temperature is between 23-26 DEG C；(3) seedless roxburgh rose tissue-cultured seedling is taken root,
It will grow tall to 4 centimetres of seedling and be transferred on root media, root media is 1/2MS+IBA0.01-0.05mg/L+
IAA0.01-0.05mg/L, can take root after 15 days, obtain seedless roxburgh rose rooted seedling, the condition of whole culture of rootage is light intensity
1000-2000Lux, hour/day of light application time 12, temperature is between 23-26 DEG C；(4) cleaning and transplanting of seedless roxburgh rose rooted seedling：
After the root of seedless roxburgh rose rooted seedling grows to more than 2 centimetres, rooted seedling is directly taken out, the culture medium attached in root is cleaned, it
After carry out acclimatization and transplantses, transplanting medium proportioning is fertile soil: perlite=5 by volume: 1, canopy internal control temperature control is noted after transplanting
Wet, temperature control is between 25-30 degree, and humidity is survived more than 80% after 15 days, and 10cm × 10cm nutrition is moved to after surviving
In bag, transplanting medium is fertile soil by volume: perlite=5: 1.
The fast propagation method of seedless roxburgh rose seedlings technical sophistication, it is difficult to grasp, cost is high, is not suitable for actual popularization and answers
With.In addition, this technology is directed to seedless roxburgh rose, rather than Rosa roxburghii (note：Seedless roxburgh rose is two different species from Rosa roxburghii).
The cultural method of seedless roxburgh rose：(1) selection on seedbed ground：Select soil layer deep, organic matter is preferable, water condition side
Just, it is not low-lying to cause flooded soil；(2) site preparation opens railway carriage or compartment：Ditched by 1.2M railway carriage or compartments face, furrow depth 32cm, soil in small, broken bits, mu applies agriculture of becoming thoroughly decomposed
1500 kilograms or composite fertilizer 120kg of family's fertilizer, seed bed preparation terminates, and disposably pours permeable in cuttage the previous day to seedbed；(3) fringe is taken
Bar：Do cuttings resource using the healthy branch of winter pruning or build maternal plant garden and do fringe bar supply, plant the seedless roxburgh rose fruit tree of 1 year,
Base portion sprouts 4-5 lopwood bars, and growing way is vigorous, and a large amount of branches can do tloig-cutlage, go after fringe, cuts fringe to fringe bar progress and stays bud, often
2 buds of fringe section, it is ensured that at least latter bud of cuttage is damaged, and another bud is survived, and fringe section configures APD root-inducing powders before cuttage
50ppm solution soaks root, and slotting base portion is immersed in solution 5 minutes, and 4-8h cuttages again are placed after leaching；(4) cuttage：Cuttage can use seedling
Bed soil cuttage and nutrition this bowl-entering soil cuttage, seedbed cutting density press 3cm × 3cm square cuttages, and every mu of cave Basic Seedling reaches 60,000
Strain；One plant of each cuttage of nutritive cube device, nutrition pot seedling can be transplanted in advance, 5 months just portables, seedbed cuttage knot after cutting survival
Shu Houyong bamboo chip arch camber epiphragmas, film edge is tight with soil, stuffy ventilative, keeps humidity in film；(5) manage：Cuttage winter
Carry out, to March in spring after Survival rate of nursery stock, temperature bottom out 28 DEG C of degree high temperature above of temperature such as occurs it is noted that taking off film ventilation,
Start simultaneously at hardening；Temperature at the beginning of 4 months is significantly raised, is not required to keep high temperature in film, can open film artificial weeding, fertilizing, watering,
Fertilising can be applied with cleaning up excrement water pours, and can convert 1.2kg urea with every 100kg water and impose to autumn, topdress 2-3 times, water nursery soil
Now white water shortage, notices that observation is watered in good time；(6) garden is gone out：By 1 year seedbed time growth management, one year seedling is up to 45-
50cm, just can go out garden transplanting.
The cultural method of the seedless roxburgh rose is directly afforested with branch cutting, it is adaptable to which the condition such as soil, moisture is non-
Chang Youyue environment.And the condition such as Karst Rocky Desertification environment soil layer is very thin, soil depletion, moisture is very severe, the technology
It can not be applicable.
The content of the invention
Lacked based on the Guizhou mountainous area current nursery of medicine-food two-purpose Special plant Rosa roxburghii and cultivation technique, prior art complexity,
It is difficult to grasp, cost is high, is not suitable for the actual popularization and application in Karst rocky desertification.The present invention provides one kind and can be used for
Quickly, easy, efficient Rosa roxburghii (Rosa roxburghii) isolated shoot method for culturing seedlings and applied to making in Rocky Desertification Control
Woods technology.Simple and effective of the present invention, quickly breeds suitable for Rosa roxburghii and applied to production practices such as Rocky Desertification Controls, can not only produce
Raw obvious economic benefit, while also having the good ecological benefits such as water and soil conservation, revegetation.
The technical solution adopted by the present invention is as follows：
A kind of Rosa roxburghii isolated shoot method for culturing seedlings, comprises the following steps：
Step (1), the selection and processing of female branch：The healthy and strong biennial and perennial Rosa roxburghii branch conduct of growth selection
Female branch of nursery, is cut into the stem section of 15~20 centimeter lengths, 1-3 bud point of every section of band；
Step (2), nursery：By the obtained stem section of step (1) moistening for the heteroauxin solution for being soaked with 50mg/L
Absorbent material wraps lower end, is then placed in culture plate or culture cup, then culture plate or culture cup are put into light transmission container
Interior, room temperature is placed, and is kept the skin wet every 1~2 day, keeps the gauze moistening of branch lower end parcel, after 15 days, the stem more than 90%
Section roots as a complete seedling；
Step (3), transplants hardening：The seedling replanting that step (2) is obtained is into nutrient bag, and transplanting medium is by volume
Fertile soil: perlite=4: 1~5: 1, control of sheltering from heat or light is 40%~60%, and temperature control is at 20~30 DEG C, after cultivating 30 days,
Obtain the Rosa roxburghii seedling that robust growth, height of seedling reach more than 30cm.
It is further preferred that the absorbent material described in step (2) is cotton, filter paper or cotton gauze.
It is further preferred that the specification of the nutrient bag described in step (3) is 10cm × 10cm~12cm × 12cm.
Robust growth, height of seedling obtained by above-mentioned Rosa roxburghii isolated shoot method for culturing seedlings reach more than 30cm Rosa roxburghii seedling in stone
Application in desertization improvement.
Robust growth, height of seedling obtained by above-mentioned method for culturing seedlings reach more than 30cm Rosa roxburghii seedling answering in Rocky Desertification Control
With its method of forestation is as follows：
Step (1), the density of plantation, site preparation specification and method：The density of plantation is 300cm × 300cm；Land preparation mode is cave
Shape, plant pit specification is cave footpath 40cm × 30cm；Site preparation requirement carried out along contour, during site preparation kind plant hole by " product, font configuration,
One layer of 3~5cm thick dry branches and fallen leaves and organic fertilizer are spread per cave simultaneously, the underground leakage of moisture is reduced, water holding capacity, increase is improved
Ground temperature；
Step (2), mode of planting seedlings and time：Inspected for acceptance for the whole good ground of step (1), it is qualified after, select it is overcast and rainy
Gas is planted seedlings again；When planting seedlings, root is hindered in finishing first, and selection of planting seedlings is in 2 months early springs to March, by " three fill out, two step on, one carries seedling "
Method, while root water need to be poured；
Step (3), fertilising：Before planting seedlings, base fertilizer is used sufficient, is used sufficient by 15kg/ plants of farm manures as base manure, treats that transplanted seedling is survived
Afterwards, supply to apply as seed manure is carried by 3.3kg/ mus of urea；
Step (4), after-culture is mended and made：To bottom class of the survival rate below 85%, after-culture is carried out in time in winter then.
It is further preferred that stony desertification is seriously regional, do not limited during preparations for afforestation by seeding row spacing.
It is further preferred that when step (2) is planted seedlings, finishing is hindered after root, to be immersed in mud or water 3~5 minutes.
Seedling replanting of the present invention can not only use nutrient bag, other have the container of similar effect or function all can, such as
The simple material such as disposal plastic cup.
Culture plate or culture cup are put into light transmission container by the present invention, main to consider to be capped, and are reduced evaporation, are prevented water
Part is quick to lose, but can not also be put into light transmission container, lid of the lid with air-vent directly on culture plate or culture cup
Son etc. slows down the evaporation of moisture, if the high humidity when, need not this operation, depending on actual conditions.
Compared with prior art, its advantage is the present invention：
(1) present invention is simple and easy to do, and cost is low, no soil pollution, efficiently, is quickly bred and applied to stony desert suitable for Rosa roxburghii
Change the production practices such as improvement, can not only produce obvious economic benefit, while also having water and soil conservation, revegetation etc. good
Ecological benefits.
(2) transplanting survival rate of the present invention has a significant effect up to more than 95% using Rocky Desertification Control, administers area vegetation
Coverage improves 6~8%, soil erosion modulus reduction by 15~20%, stony desertification area ratio reduction by 10~15%.
Embodiment
With reference to embodiment, the present invention is described in further detail.
It will be understood to those of skill in the art that the following example is merely to illustrate the present invention, and it should not be regarded as limiting this hair
Bright scope.In the examples where no specific technique or condition is specified, according to the technology or condition described by document in the art
Or carried out according to product description.Agents useful for same or the unreceipted production firm person of instrument, are that can be obtained by buying
Conventional products.
Embodiment 1
A kind of Rosa roxburghii isolated shoot method for culturing seedlings, comprises the following steps：
Step (1), the selection and processing of female branch：The healthy and strong biennial and perennial Rosa roxburghii branch conduct of growth selection
Female branch of nursery, is cut into the stem section of 15 centimeter lengths, 1 bud point of every section of band；
Step (2), nursery：By the obtained stem section of step (1) moistening for the heteroauxin solution for being soaked with 50mg/L
Absorbent material wraps lower end, is then placed in culture cup, then culture plate or culture cup are put into light transmission container, and room temperature is put
Put, kept the skin wet every 1 day, keep the gauze moistening of branch lower end parcel, after 15 days, the stem section more than 90% is rooted into
For a complete seedling；Described absorbent material is cotton, filter paper or cotton gauze；
Step (3), transplants hardening：The seedling replanting that step (2) is obtained is into nutrient bag, and transplanting medium is by volume
Fertile soil: perlite=4: 1, control of sheltering from heat or light is 40%~45%, and temperature control after cultivating 30 days, is grown at 20 DEG C
Healthy and strong, height of seedling reaches more than 30cm Rosa roxburghii seedling；The specification of described nutrient bag is 10cm × 10cm.
Robust growth, height of seedling obtained by the present embodiment method for culturing seedlings reach more than 30cm Rosa roxburghii seedling in Rocky Desertification Control
Application, its method of forestation is as follows：
Step (1), the density of plantation, site preparation specification and method：The density of plantation is 300cm × 300cm；Land preparation mode is cave
Shape, plant pit specification is cave footpath 40cm × 30cm；Site preparation requirement is carried out along contour, and kind plant hole is configured by triangle disposition during site preparation,
One layer of 3cm thick dry branches and fallen leaves and organic fertilizer are spread per cave simultaneously, the underground leakage of moisture is reduced, water holding capacity, increase ground is improved
Temperature；
Step (2), mode of planting seedlings and time：Inspected for acceptance for the whole good ground of step (1), it is qualified after, select it is overcast and rainy
Gas is planted seedlings again；When planting seedlings, root is hindered in finishing first, and selection of planting seedlings is in 2 months early springs to March, by " three fill out, two step on, one carries seedling "
Method, while root water need to be poured；
Step (3), fertilising：Before planting seedlings, base fertilizer is used sufficient, is used sufficient by 15kg/ plants of farm manures as base manure, treats that transplanted seedling is survived
Afterwards, supply to apply as seed manure is carried by 3.3kg/ mus of urea；
Step (4), after-culture is mended and made：To bottom class of the survival rate below 85%, after-culture is carried out in time in winter then.
The present embodiment transplanting survival rate has a significant effect up to 95.5% using Rocky Desertification Control, administers area vegetation and covers
Cover degree improves 6.2%, soil erosion modulus reduction by 18%, stony desertification area ratio reduction by 13%.
Embodiment 2
A kind of Rosa roxburghii isolated shoot method for culturing seedlings, comprises the following steps：
Step (1), the selection and processing of female branch：The healthy and strong biennial and perennial Rosa roxburghii branch conduct of growth selection
Female branch of nursery, is cut into the stem section of 20 centimeter lengths, 3 bud points of every section of band；
Step (2), nursery：By the obtained stem section of step (1) moistening for the heteroauxin solution for being soaked with 50mg/L
Absorbent material wraps lower end, is then placed in culture plate, then culture plate or culture cup are put into light transmission container, and room temperature is put
Put, kept the skin wet every 2 days, keep the gauze moistening of branch lower end parcel, after 15 days, the stem section more than 90% is rooted into
For a complete seedling；Described absorbent material is cotton, filter paper or cotton gauze；
Step (3), transplants hardening：The seedling replanting that step (2) is obtained is into nutrient bag, and transplanting medium is by volume
Fertile soil: perlite=5: 1, control of sheltering from heat or light is 55%~60%, and temperature control after cultivating 30 days, is grown at 30 DEG C
Healthy and strong, height of seedling reaches more than 30cm Rosa roxburghii seedling；The specification of described nutrient bag is 12cm × 12cm.
Robust growth, height of seedling obtained by the present embodiment method for culturing seedlings reach more than 30cm Rosa roxburghii seedling in Rocky Desertification Control
Application, its method of forestation is as follows：
Step (1), the density of plantation, site preparation specification and method：The density of plantation is 300cm × 300cm；Land preparation mode is cave
Shape, plant pit specification is cave footpath 40cm × 30cm；Site preparation requirement is carried out along contour, and kind plant hole is configured by triangle disposition during site preparation,
One layer of 5cm thick dry branches and fallen leaves and organic fertilizer are spread per cave simultaneously, the underground leakage of moisture is reduced, water holding capacity, increase ground is improved
Temperature；
Step (2), mode of planting seedlings and time：Inspected for acceptance for the whole good ground of step (1), it is qualified after, select it is overcast and rainy
Gas is planted seedlings again；When planting seedlings, root is hindered in finishing first, then will be hindered root and is immersed in the water 3 minutes, plants seedlings selection on March in early spring
Ten days, by the method for " three fill out, two step on, one carries seedling ", while root water need to be poured；
Step (3), fertilising：Before planting seedlings, base fertilizer is used sufficient, is used sufficient by 15kg/ plants of farm manures as base manure, treats that transplanted seedling is survived
Afterwards, supply to apply as seed manure is carried by 3.3kg/ mus of urea；
Step (4), after-culture is mended and made：To bottom class of the survival rate below 85%, after-culture is carried out in time in winter then.
The present embodiment transplanting survival rate has a significant effect up to 97% using Rocky Desertification Control, administers area's vegetative coverage
Degree improves 7.2%, soil erosion modulus reduction by 19.5%, stony desertification area ratio reduction by 15%.
Embodiment 3
A kind of Rosa roxburghii isolated shoot method for culturing seedlings, comprises the following steps：
Step (1), the selection and processing of female branch：The healthy and strong biennial and perennial Rosa roxburghii branch conduct of growth selection
Female branch of nursery, is cut into the stem section of 18 centimeter lengths, 2 bud points of every section of band；
Step (2), nursery：By the obtained stem section of step (1) moistening for the heteroauxin solution for being soaked with 50mg/L
Absorbent material wraps lower end, is then placed in culture plate, then culture plate or culture cup are put into light transmission container, and room temperature is put
Put, kept the skin wet every 1.3 days, keep the gauze moistening of branch lower end parcel, after 15 days, the stem section more than 90% roots
As a complete seedling；Described absorbent material is cotton, filter paper or cotton gauze；
Step (3), transplants hardening：The seedling replanting that step (2) is obtained is into nutrient bag, and transplanting medium is by volume
Fertile soil: perlite=4.2: 1, control of sheltering from heat or light is 50%~55%, and temperature control after cultivating 30 days, is obtained at 24~25 DEG C
More than 30cm Rosa roxburghii seedling is reached to robust growth, height of seedling；The specification of described nutrient bag is 11cm × 11cm.
Robust growth, height of seedling obtained by the present embodiment method for culturing seedlings reach more than 30cm Rosa roxburghii seedling in Rocky Desertification Control
Application, its method of forestation is as follows：
Step (1), the density of plantation, site preparation specification and method：The density of plantation is 300cm × 300cm；Land preparation mode is cave
Shape, plant pit specification is cave footpath 40cm × 30cm；Site preparation requirement is carried out along contour, and kind plant hole is configured by triangle disposition during site preparation,
One layer of 3.7cm thick dry branches and fallen leaves and organic fertilizer are spread per cave simultaneously, the underground leakage of moisture is reduced, water holding capacity, increase is improved
Ground temperature；
Step (2), mode of planting seedlings and time：Inspected for acceptance for the whole good ground of step (1), it is qualified after, select it is overcast and rainy
Gas is planted seedlings again；When planting seedlings, root is hindered in finishing first, and finishing is hindered after root, immersed in mud 5 minutes, and selection of planting seedlings is in early spring 2
Month, by the method for " three fill out, two step on, one carries seedling ", while root water need to be poured；
Step (3), fertilising：Before planting seedlings, base fertilizer is used sufficient, is used sufficient by 15kg/ plants of farm manures as base manure, treats that transplanted seedling is survived
Afterwards, supply to apply as seed manure is carried by 3.3kg/ mus of urea；
Step (4), after-culture is mended and made：To bottom class of the survival rate below 85%, after-culture is carried out in time in winter then.
The present embodiment transplanting survival rate has a significant effect up to 99% using Rocky Desertification Control, administers area's vegetative coverage
Degree improves 8%, soil erosion modulus reduction by 19.6%, stony desertification area ratio reduction by 14.2%.
Embodiment 4
A kind of Rosa roxburghii isolated shoot method for culturing seedlings, comprises the following steps：
Step (1), the selection and processing of female branch：The healthy and strong biennial and perennial Rosa roxburghii branch conduct of growth selection
Female branch of nursery, is cut into the stem section of 16-17 centimeter lengths, 2-3 bud point of every section of band；
Step (2), nursery：By the obtained stem section of step (1) moistening for the heteroauxin solution for being soaked with 50mg/L
Absorbent material wraps lower end, is then placed in culture cup, then culture plate or culture cup are put into light transmission container, and room temperature is put
Put, kept the skin wet every 1.8 days, keep the gauze moistening of branch lower end parcel, after 15 days, the stem section more than 90% roots
As a complete seedling；Described absorbent material is cotton, filter paper or cotton gauze；
Step (3), transplants hardening：The seedling replanting that step (2) is obtained is into nutrient bag, and transplanting medium is by volume
Fertile soil: perlite=4.5: 1, control of sheltering from heat or light is 48%~50%, and temperature control after cultivating 30 days, is obtained at 26~28 DEG C
More than 30cm Rosa roxburghii seedling is reached to robust growth, height of seedling；The specification of described nutrient bag is 10cm × 10cm.
Robust growth, height of seedling obtained by the present embodiment method for culturing seedlings reach more than 30cm Rosa roxburghii seedling in Rocky Desertification Control
Application, its method of forestation is as follows：
Step (1), the density of plantation, site preparation specification and method：Stony desertification is seriously regional, is not limited during preparations for afforestation by seeding row spacing
System；Land preparation mode is cave-shaped, and plant pit specification is cave footpath 40cm × 30cm；Site preparation requirement is carried out along contour, kind plant hole during site preparation
Configured by triangle disposition, while spreading one layer of 4.5cm thick dry branches and fallen leaves and organic fertilizer per cave, reduce the underground leakage of moisture, carry
High water holding capacity, increases ground temperature；
Step (2), mode of planting seedlings and time：Inspected for acceptance for the whole good ground of step (1), it is qualified after, select it is overcast and rainy
Gas is planted seedlings again；When planting seedlings, root is hindered in finishing first, and finishing is hindered after root, immersed in mud 4 minutes, and selection of planting seedlings is in early spring 3
The middle of the month, by the method for " three fill out, two step on, one carries seedling ", while root water need to be poured；
Step (3), fertilising：Before planting seedlings, base fertilizer is used sufficient, is used sufficient by 15kg/ plants of farm manures as base manure, treats that transplanted seedling is survived
Afterwards, supply to apply as seed manure is carried by 3.3kg/ mus of urea；
Step (4), after-culture is mended and made：To bottom class of the survival rate below 85%, after-culture is carried out in time in winter then.
The present embodiment transplanting survival rate has a significant effect up to 99.5% using Rocky Desertification Control, administers area vegetation and covers
Cover degree improves 6.3%, soil erosion modulus reduction by 15.5%, stony desertification area ratio reduction by 10%.
The general principle and principal character and advantages of the present invention of the present invention has been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the simply explanation described in above-described embodiment and specification is originally
The principle of invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (7)
1. a kind of Rosa roxburghii isolated shoot method for culturing seedlings, it is characterised in that comprise the following steps：
Step (1), the selection and processing of female branch：The healthy and strong biennial and perennial Rosa roxburghii branch of growth selection is used as nursery
Female branch, be cut into the stem section of 15~20 centimeter lengths, 1-3 bud point of every section of band；
Step (2), nursery：By water suction of the obtained stem section of step (1) with the moistening for the heteroauxin solution for being soaked with 50mg/L
Material wraps lower end, is then placed in culture plate or culture cup, and room temperature is placed, and is kept the skin wet every 1~2 day, keeps branch
The gauze moistening of bar lower end parcel, after 15 days, the stem section more than 90% roots as a complete seedling；
Step (3), transplants hardening：The seedling replanting that step (2) is obtained is into nutrient bag, and transplanting medium is humic by volume
Soil: perlite=4: 1~5: 1, control of sheltering from heat or light is 40%~60%, and temperature control after cultivating 30 days, is obtained at 20~30 DEG C
Robust growth, height of seedling reach more than 30cm Rosa roxburghii seedling.
2. Rosa roxburghii isolated shoot method for culturing seedlings according to claim 1, it is characterised in that the water-absorption material described in step (2)
Expect for cotton, filter paper or cotton gauze.
3. Rosa roxburghii isolated shoot method for culturing seedlings according to claim 1, it is characterised in that the nutrient bag described in step (3)
Specification be 10cm × 10cm~12cm × 12cm.
4. reached based on the robust growth obtained by the Rosa roxburghii isolated shoot method for culturing seedlings described in claim 1 or 2 or 3, height of seedling
Application of more than the 30cm Rosa roxburghii seedling in Rocky Desertification Control.
5. the robust growth, height of seedling obtained by Rosa roxburghii isolated shoot method for culturing seedlings according to claim 4 reach more than 30cm
Application of the Rosa roxburghii seedling in Rocky Desertification Control, it is characterised in that method of forestation is as follows：
Step (1), the density of plantation, site preparation specification and method：The density of plantation is 300cm × 300cm；Land preparation mode is cave-shaped, tree
Cave specification is cave footpath 40cm × 30cm；Site preparation requirement is carried out along contour, and kind plant hole is configured by triangle disposition during site preparation, while often
Spread the thick dry branches and fallen leaves and organic fertilizer of one layer of 3~5cm in cave；
Step (2), mode of planting seedlings and time：Inspected for acceptance for the whole good ground of step (1), it is qualified after, selection rainy weather again
Planted seedlings；When planting seedlings, root is hindered in finishing first, and selection of planting seedlings is in 2 months early springs to March, by the side of " three fill out, two step on, one carries seedling "
Method, while root water need to be poured；
Step (3), fertilising：Before planting seedlings, base fertilizer is used sufficient, is used sufficient by 15kg/ plants of farm manures as base manure, after transplanted seedling is survived,
Supply to apply as seed manure is carried by 3.3kg/ mus of urea；
Step (4), after-culture is mended and made：To bottom class of the survival rate below 85%, after-culture is carried out in time in winter then.
6. the robust growth, height of seedling obtained by Rosa roxburghii isolated shoot method for culturing seedlings according to claim 5 reach more than 30cm
Application of the Rosa roxburghii seedling in Rocky Desertification Control, it is characterised in that stony desertification is seriously regional, is not limited during preparations for afforestation by seeding row spacing
System.
7. the robust growth, height of seedling obtained by Rosa roxburghii isolated shoot method for culturing seedlings according to claim 5 reach more than 30cm
Application of the Rosa roxburghii seedling in Rocky Desertification Control, it is characterised in that when step (2) is planted seedlings, finishing is hindered after root, to immerse mud or
3~5 minutes in water.
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| CN105941060A (en) * | 2016-06-06 | 2016-09-21 | 遵义县万佳彰中药材有限公司 | Mountain agriculture medicine and grain interplanting method |
| CN107624635A (en) * | 2017-10-18 | 2018-01-26 | 贵州省金黔草农业有限公司 | A kind of method of Rosa roxburghii tissue-cultured seedling low temperature hardening |
| CN110637665B (en) * | 2019-11-01 | 2022-03-25 | 湖南农业大学 | Rapid cutting propagation method for citrus limonum |
| CN114287248A (en) * | 2021-12-08 | 2022-04-08 | 贵州黔西新特农业开发有限公司 | Insect-preventing disease-resistant seedling raising method for roxburgh rose |
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| CN1806525A (en) * | 2006-01-08 | 2006-07-26 | 陈运章 | Method for introducing, domesticating and cultivating superior high-yield seedling of mountain area precious wild thorn |
| CN102986535B (en) * | 2012-12-14 | 2014-03-26 | 西南林业大学 | Fast propagation method of seedless roxburgh rose seedlings |
| CN103070070A (en) * | 2013-01-10 | 2013-05-01 | 普定县元贞农业发展有限责任公司 | Cultivation method of seedless roxburgh roses |
| CN103229692A (en) * | 2013-05-05 | 2013-08-07 | 安顺市西秀区胜学原生态种养植生态园 | Cultivation method for seedless roxburgh roses |
| CN103416276B (en) * | 2013-08-29 | 2014-10-22 | 贵州兴黔伟业生态科技开发有限公司 | Roxburgh rose seedling raising method |
| CN103583310A (en) * | 2013-11-15 | 2014-02-19 | 贵州师范大学 | Drought resisting and forest planting method for karst region difficult site |
| CN103814805B (en) * | 2014-03-09 | 2015-09-09 | 贵州省生物研究所 | A kind of without seed Rosa roxburghii cuttage breeding method |
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