CN107624635A - A kind of method of Rosa roxburghii tissue-cultured seedling low temperature hardening - Google Patents
A kind of method of Rosa roxburghii tissue-cultured seedling low temperature hardening Download PDFInfo
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- CN107624635A CN107624635A CN201710970180.XA CN201710970180A CN107624635A CN 107624635 A CN107624635 A CN 107624635A CN 201710970180 A CN201710970180 A CN 201710970180A CN 107624635 A CN107624635 A CN 107624635A
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- rosa roxburghii
- tissue
- cultured seedling
- seedling
- hardening
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Abstract
A kind of method of Rosa roxburghii tissue-cultured seedling low temperature hardening, first being removed in the environment temperature that Rosa roxburghii tissue-cultured seedling grows from tissue-cultured seedling, individually carry out closed alcohol sprinkling, tissue-culture container seedling is moved into Low- temperature culture room after the completion of potassium permanganate+formaldehyde fumigation, culture room temperature is first down to 15 DEG C 18 DEG C, after cultivating 5 7d, treat that Rosa roxburghii tissue-cultured seedling blade is gradually deepened by light green, stem apex no longer sprouts new tender shoots, stem section surface occur it is a little it is reflective after, culture room temperature is adjusted to 10 DEG C 13 DEG C, and illumination is adjusted to 1500 1800Lx, continues to cultivate 8 10d.It is reflective to treat that tissue culture seeding stem segment occurs, seedling stops growing, taking-up Rosa roxburghii tissue-cultured seedling, and on the seedbed in ordinary greenhouse, temperature is maintained at 18 28 DEG C, and humidity more than 85%, greenhouse ventilation is good, can improve the tolerance of Rosa roxburghii hardening survival rate and seedling stage to disease.The survival rate of Rosa roxburghii tissue culture hardening can be improved, greatly reduces the pollution rate during hardening, accelerates Rosa roxburghii artificial breeding, it is significant.
Description
Technical field
The present invention relates to characteristic fruit forest zones fast seedling raising field, a kind of method of training tissue culture seedling is particularly belonged to.
Background technology
Rosa roxburghii (Rosa roxburghii Tratt. undershrub, also known as oblonga) are climbed up by holding on to for rose family Rosa is perennial
Son, Rosa roxburghii, single-lobe rosa roxburghii etc., are distributed in some areas such as Guizhou In China, Sichuan, Yunnan, Hunan and Tibet, have higher
Economy, gardening and health value.In recent years, as people enter to the pay attention to day by day of health care etc., Rosa roxburghii as economic forest
Row large-scale planting has also progressively been spread out in provinces such as Yunnan, Guizhou.Seedling demand is increasing, quickly, efficiently obtains in a short time
A large amount of Rosa roxburghii seedlings are obtained, it is very urgent to improve seedling transplanting survival rate.
Tissue culture technique has turned into Rosa roxburghii extension population, and standardization, large-scale planting Rosa roxburghii are more convenient and feasible
Method.Can hardening survival rate height direct relation Rosa roxburghii tissue cultures successful.During conventional hardening, often using strong light
The modes such as hardening, natural light transition hardening, Small plastic shed hardening.The present invention is based on plant at low ambient temperatures, soluble sugar content
Increase, water content is reduced, and enzymatic activity reduces, and stomata is closed, and stress resistance of plant enhancing is ultimately resulted in, to reach raising acclimatization and transplantses
The purpose of survival rate.With this method, Rosa roxburghii acclimatization and transplantses survival rate can be made to improve to 95%, seedling-slowing stage substantially shortens after transplanting,
Reduced by root rot, stem rot infection probability, greatly improve acclimatization and transplantses survival rate.
The content of the invention
[1] purpose of the present invention is to establish a set of Rosa roxburghii tissue-cultured seedling low temperature hardening technology, can greatly improve Rosa roxburghii tissue culture
Survival rate in hardening, while the pollution rate during hardening is greatly reduced, the technical program expands accelerating Rosa roxburghii artificial breeding
Big metaplasia production provides technology guarantee, significant to Rocky Desertification Control, water and soil conservation.
[2] present invention can be realized by following technology
[3] step 1：The Rosa roxburghii bottle seedling for treating acclimatization and transplantses is moved into hardening culturing room, sprays depositing dust, potassium permanganate with 75% alcohol
With the closed stifling 20min of formaldehyde；
[4] step 2：Rosa roxburghii tissue-cultured seedling after will be stifling is in 15-18 DEG C of culturing room's environment temperature, intensity of illumination 1800-
2000Lx, light application time 12h/d, 5-7d is cultivated in draughty environment；
[5] step 3：By 5-7d Low- temperature culture, Rosa roxburghii tissue-cultured seedling leaf color gradually deepens, and height of seedling growing way slows down or even stopped
Growth.Cultivation temperature is turned down to 10-13 DEG C, illumination is adjusted to 1500-1800Lx, is continued to cultivate 8-10d, is treated tissue-cultured seedling cutin
There is a little reflective, the obvious spinule of leaf margin appearance, after height of seedling stops growing substantially, taking-up Rosa roxburghii tissue-cultured seedling in layer；
[6] step 4：Rosa roxburghii tissue-cultured seedling is carefully taken out with tweezers, 60min is soaked in 800 times of carbendazim thimerosals, uses wet hair
Towel covers blade-section, is planted after dry in the shade root and stem section surface moisture in the good hardening matrix of configured in advance.Whole transplanting
During, humidity must not be less than 85%, and ventilation condition is good；
[7] in the step [3], before Rosa roxburghii training tissue culture seedling, it is necessary to spray depositing dust, potassium permanganate and formaldehyde by 75% alcohol
Closed stifling 20min, to reach tissue culture bottle outer surface and culturing room's sterilization purpose；
[8] in the step [4], Rosa roxburghii tissue-cultured seedling must be 15-18 DEG C, intensity of illumination 1800-2000Lx through environment temperature, illumination
Time 12h/d culture environment transition culture 5-7d；
[9] in the step [4], there is leaf color and gradually deepened in Rosa roxburghii tissue-cultured seedling, and leaf margin spinule is gradually obvious, and stem section surface occurs
A little reflective, Seedling height growth slowly even stops growing；
[10] in the step [5], the appearance of Rosa roxburghii tissue culture seeding stem segment surface is a little reflective, and height of seedling stops growing substantially, and leaf margin is small
Thorn is obvious；
[11] in the step [5], culture room temperature is adjusted to 10-13 DEG C, and illumination is adjusted to 1500-1800Lx, light application time
12h/d, cultivate 8-10d；
[12] in the step [6], Rosa roxburghii tissue-cultured seedling soaks 60min in 800 times of carbendazim thimerosals；
[13] in the step [6], transplanting medium with the vermiculite after sterilized, fertile soil, graininess farm manure by a certain percentage
Mixing, specific volume proportion is vermiculite (10%-20%)+fertile soil (40%-50%)+graininess farm manure (40%-50%)；
Using the beneficial effect of technical solution of the present invention：Traditional Rosa roxburghii tissue culture hardening off method is changed, there is hardening survival rate height,
Robust seedling, the characteristics such as seedling-slowing stage is short, uniformity is good after seedling hardening, and excessive growth, yellow, false situation living are few.The application of this programme, not only
Be advantageous to carry out standardization desert treatment using Rosa roxburghii, while technical support can be provided for the diversification application of Rosa roxburghii, produce good
Good economy, society and ecological benefits.
Embodiment
Embodiment 1
1st, before Rosa roxburghii training tissue culture seedling, select that upgrowth situation is good, and leaf color is light green to blackish green, height of seedling 3-5cm bottle seedling frames up standby
With；
2nd, the Rosa roxburghii bottle seedling for treating acclimatization and transplantses is moved into hardening culturing room, sprays depositing dust with 75% alcohol, potassium permanganate and formaldehyde are close
Stifling 20min is closed, has reached and has killed body surface mushroom purpose；
3rd, the Rosa roxburghii tissue-cultured seedling after will be stifling is in 15-18 DEG C of environment temperature, intensity of illumination 1800-2000Lx, light application time 12h/
D, cultivates 5-7d in draughty environment, in incubation, it is seen that the Rosa roxburghii tissue-cultured seedling speed of growth is slow, and part is excessively young tender
Bottle seedling height of seedling there is obvious stagnant situation；
4th, by 5-7d transition culture, there is leaf color and deepened in Rosa roxburghii tissue-cultured seedling, and leaf margin spinule is gradually obvious, stem section surface and leaf
Face occurs a little reflective；
5th, culture room temperature is adjusted to 10-13 DEG C, and illumination is adjusted to 1500-1800Lx, light application time 12h/d, cultivates 8-10d, this
In one stage incubation, Rosa roxburghii growth is substantially suppressed, and blade face and the appearance of stem section surface are reflective, and leaf margin spinule is obvious；Move
Go out Rosa roxburghii tissue-cultured seedling to service sink.
6th, Rosa roxburghii tissue-cultured seedling is taken out, soaks 60min in 800 times of carbendazim thimerosals, blade and stem section are covered with wet towel
Part, it is stand-by after the surface moisture that dries in the shade；
7th, transplanting medium is mixed by a certain percentage with the vermiculite after sterilized, fertile soil, graininess farm manure, specific volume proportion
For vermiculite (10%-20%)+fertile soil (40%-50%)+graininess farm manure (40%-50%)；
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention be not limited thereto, it is any ripe
Know those skilled in the art the invention discloses technical scope in, do not depart from the present invention principle under the premise of, can be easily
The change or replacement made, are included within the scope of protection of the invention.
Claims (6)
- A kind of 1. method of Rosa roxburghii tissue-cultured seedling low temperature hardening, it is characterised in that it is made up of following step：Step 1：The Rosa roxburghii tissue-cultured seedling for treating acclimatization and transplantses is moved into hardening culturing room, with alcohol atomizing de-dusting, potassium permanganate and first The closed stifling 20min of aldehyde；Step 2：Will it is stifling after Rosa roxburghii tissue-cultured seedling in 15-18 DEG C of room temperature of culture, intensity of illumination 1800-2000Lx, during illumination Between 12h/d, cultivate 5-7d in draughty environment；Step 3：By 5-7d Low- temperature culture, cultivation temperature to be turned down to 10-13 DEG C, illumination is adjusted to 1500-1800Lx, Continue to cultivate 8-10d；Step 4：Rosa roxburghii tissue-cultured seedling is carefully taken out with tweezers, soaks 60min in 800 times of carbendazim thimerosals, with wet towel lid Plant in the good hardening matrix of configured in advance, move after firmly Stem Segments of Rosa roxburghii and blade-section, stem section of drying in the shade and root surface moisture During cultivation, humidity must not be less than 85%, and ventilation condition is good.
- 2. the method for Rosa roxburghii tissue-cultured seedling low temperature hardening according to claim 1, it is characterised in that：, must in the step 1 Tissue culture bottle outer surface and culturing room must be sterilized, spray depositing dust, potassium permanganate and the closed stifling 20min of formaldehyde with 75% alcohol, with Reach disinfection purpose.
- 3. the method for Rosa roxburghii tissue-cultured seedling low temperature hardening according to claim 1, it is characterised in that：Training in the step 2 Room temperature is supported, illumination, cultivates 15-18 DEG C of room temperature, under the conditions of intensity of illumination 1800-2000Lx, blade occurs in Rosa roxburghii tissue-cultured seedling Surface leaf color deepens, and stem section appearance is a little reflective, and Seedling height growth slowly even stops growing and carries out third step again.
- 4. the method for Rosa roxburghii tissue-cultured seedling low temperature hardening according to claim 1, it is characterised in that：In the step 3, drop Under low cultivation temperature and illumination condition, temperature is turned down to 10-13 DEG C, and illumination is adjusted to 1500-1800Lx, continues to cultivate 8-10d, Tissue-cultured seedling leaf color is set gradually to deepen, leaf margin spinule is obvious, and reflective, removal Rosa roxburghii tissue-cultured seedling to service sink occurs in stem section surface.
- 5. the method for Rosa roxburghii tissue-cultured seedling low temperature hardening according to claim 1, it is characterised in that：In the step 4, thorn Pears tissue-cultured seedling soaks 60min in 800 times of carbendazim thimerosals.
- 6. the method for Rosa roxburghii tissue-cultured seedling low temperature hardening according to claim 1, it is characterised in that：In the step 4, move Matrix is planted as the vermiculite after sterilized, fertile soil, graininess farm manure mixes in proportion, is vermiculite 10%-20%+ by volume proportion Fertile soil 40%-50%+ graininess farm manures 40%-50%.
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| CN201710970180.XA CN107624635A (en) | 2017-10-18 | 2017-10-18 | A kind of method of Rosa roxburghii tissue-cultured seedling low temperature hardening |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111084090A (en) * | 2020-02-18 | 2020-05-01 | 美尚生态景观股份有限公司 | Method for hardening and transplanting wheat and plum tissue culture seedlings |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1586144A (en) * | 2004-08-06 | 2005-03-02 | 上海光兆植物速生技术有限公司 | Method for storaging seedling in winter for poplar tissue cutlivating seedling |
| CN102986535A (en) * | 2012-12-14 | 2013-03-27 | 西南林业大学 | Fast propagation method of seedless roxburgh rose seedlings |
| CN104770177A (en) * | 2015-04-10 | 2015-07-15 | 贵州师范大学 | Rosa roxburghii ex-vivo twig culturing method and application thereof in stony desertification control |
| CN105706723A (en) * | 2016-03-15 | 2016-06-29 | 毕节市中药研究所 | Low temperature seedling hardening method for psammosilene tunicoides tissue culture seedling |
| CN106417027A (en) * | 2016-10-14 | 2017-02-22 | 贵州万重山生态农业开发有限公司 | Low-temperature seedling hardening method for bletilla striata tissue culture seedlings |
-
2017
- 2017-10-18 CN CN201710970180.XA patent/CN107624635A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1586144A (en) * | 2004-08-06 | 2005-03-02 | 上海光兆植物速生技术有限公司 | Method for storaging seedling in winter for poplar tissue cutlivating seedling |
| CN102986535A (en) * | 2012-12-14 | 2013-03-27 | 西南林业大学 | Fast propagation method of seedless roxburgh rose seedlings |
| CN104770177A (en) * | 2015-04-10 | 2015-07-15 | 贵州师范大学 | Rosa roxburghii ex-vivo twig culturing method and application thereof in stony desertification control |
| CN105706723A (en) * | 2016-03-15 | 2016-06-29 | 毕节市中药研究所 | Low temperature seedling hardening method for psammosilene tunicoides tissue culture seedling |
| CN106417027A (en) * | 2016-10-14 | 2017-02-22 | 贵州万重山生态农业开发有限公司 | Low-temperature seedling hardening method for bletilla striata tissue culture seedlings |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111084090A (en) * | 2020-02-18 | 2020-05-01 | 美尚生态景观股份有限公司 | Method for hardening and transplanting wheat and plum tissue culture seedlings |
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Application publication date: 20180126 |