CN104770177A - Rosa roxburghii ex-vivo twig culturing method and application thereof in stony desertification control - Google Patents
Rosa roxburghii ex-vivo twig culturing method and application thereof in stony desertification control Download PDFInfo
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Abstract
The invention particularly relates to an afforestation technique for quick, simple, convenient and efficient Rosa roxburghii ex-vivo twig culturing and stony desertification control, and belongs to the technical field of fruit tree biology. The afforestation technique has the advantages that the simplicity and convenience are realized, the implementation is easy, the cost is low, the soil pollution is avoided, and the efficiency is high; the afforestation technique is suitable for the production practices, such as Rosa roxburghii quick propagation and stony desertification control; the transplanting survival rate reaches more than 95%, the obvious effect is realized in the stony desertification control, the vegetation coverage of the controlled area is improved by 6-8%, the soil erosion modulus is reduced by 15-20%, and the ratio of stony desertification area is reduced by 10-15%; the obvious economic benefit is realized, and the good economic benefits on water and soil conservancy, vegetation restoration and the like are realized.
Description
Technical field
The invention belongs to fruit tree biological technical field, be specifically related to one and may be used for quick, easy, efficient Rosa roxburghii (Rosa roxburghii) isolated shoot seedling-cultivating method and be applied to the afforestation technology in Rocky Desertification Control.
Background technology
Rosa roxburghii (Rosa roxbunghii) is rosaceous plant, and wild undershrub, is born in the endroit of height above sea level 500-2500 rice, cheuch, roadside and shrubbery, is the natural wild fruit on the ground such as Guizhou, Mountain Area of Western Hubei Province, the Liangshan Mountain, Mianning mountain area.Fruit is the precious fruit of a kind of rare nutrition with healthcare effect.The harvest time of Rosa roxburghii was less than 30 days.Perverse pears are open pink, redness or wine-colored flower April to June, and the summer flower autumn is real.Fruit mostly is oblate spherical, and transverse diameter is generally 2-4 centimetre, and August is to fruit maturation in September, yellow, is sometimes with blush.Pulp is crisp, has dense fragranced after maturation.The little corn of close life on pericarp, custom is referred to as " Rosa roxburghii ".Containing abundant vitamin C in fruit, be called as " king of Vc ".Tannin 500-1000 milligram; Containing acid about 1 gram; General about 4 grams of total reducing sugar amount.
Karst (Karst) i.e. karst, be water to solublerock, as carbonate rock, gypsum, rock salt etc. carry out based on chemical corrosion, the general name of the phenomenon that the erosion of flowing water, the mechanisms such as erosion and avalanche of diving produce for auxiliary geologic process.By landforms that karsitification causes, claim karst landform, be also karst features.
Under stony desertification (Rocky Desertification) refers to the natural background of extremely growing at the torrid zone, moistening, the subhumid climate condition in subtropics and karst, disturb by human activity, surface vegetation is wrecked, soil is caused seriously to run off, the land deterioration phenomenon of the exposed or gravel buildup of basement rock large area is also the extreme form of karst area land deterioration.
Rosa roxburghii (Rosa roxbunghii Tratt) is the perennial machaka of the rose family.Rosa roxburghii is the distinctive wild resource in the Yunnan-Guizhou Plateau and Pan Xi plateau, and take Guizhou as main product, Yunnan takes second place, the southeast, river, North-west Guangxi, the western Hunan, South-West Hubei and Shan Nan all have distribution.The medical value of Rosa roxburghii is very high, and its flower, leaf fruit and seed all can be used as medicine, and have stomach invigorating, help digestion, nourish and effect of antidiarrheal.Particularly Rosa roxburghii is rich in hepatocuprein (be called for short S0D), also has antiviral, radiation-resistant effect, in cardiovascular, digestive system and various tumor disease are prevented and treated, applies very extensive.The fruit of Rosa roxburghii is also the first-class raw material of processing health food simultaneously, and fruit is rich in sugar, vitamin, carotin, organic acid and 20 several amino acids, more than 10 plants the trace element useful to human body.Especially Vitamin C content is high, is the highest in current fruit, and content 841.58 ~ 3541.13 milligrams in every 100 grams of fresh fruits, has the laudatory title of " king of vitamin C ".
Rosa roxburghii is the species that resistance is very strong simultaneously, can grow very well in Karst Rocky Desertification adverse circumstances.Visible, research and development Rosa roxburghii nursery and stony desertification environment planting technology not only can develop Rosa roxburghii cultivates and processing industry, for consumer provides the health food of high-quality, simultaneously to improving Karst Rocky Desertification ecotope, to improve the living condition of the vast mountain area masses significant.But up to now, the nursery of Rosa roxburghii and stony desertification environment planting technology demand are very urgent, and especially the afforestation technology of Rosa roxburghii in Rocky Desertification Control is blank substantially.
Cuttage technique: cuttage also claims cutting is a kind of conventional propagation method cultivated plants.Can the stem, leaf, root, bud etc. (claiming cuttings on gardening) of clip plant, or insert in soil, Sha Zhong, or be immersed in water, just can plant after by the time taking root, make it to become independently newly plant.In agriculture and forestry are produced, during different plant cuttage, there is different demand to condition.Understand and comply with their demand, higher breeding success rate could be obtained.
Stock growing technology: stock growing technology refers to and to go out in the whole cultivating process in garden involved every technical measures getting seedling from propagating materials, comprise selection of seedling, whole, dig cave, fertilising, earthing, water, multiple link such as to manage and protect.
Without the method for seed Rosa roxburghii seedling fast-propagation, comprise axillary bud deriving, plantlet in vitro is taken root, to take root the steps such as transplantation of seedlings, specifically: the Primary culture of (1) axillary bud deriving, choose the excellent raw then tender stem without seed Rosa roxburghii, after tender stem first being used running water 10-15 minute, the stem section being cut into about 5cm carries out disinfection, sterilization employing 75% alcohol and 0.1% mercuric chloride combined sterilizing, alcohol 30 seconds, mercuric chloride 8 ~ 12 minutes, with aseptic water washing 3-4 time after sterilization, the moisture in stem section is blotted with the filter paper of sterilizing, be cut into the stem section of band 1 axil again, oblique cutting is on axillalry bud Primary culture base, axil upward, ensure that axil place can obtain sufficient illumination, Primary culture base is MS+6-BA0.5-2.0mg/L+IBA0.1-0.3mg/L, axillalry bud is induced from axil position after 10 days, continue to cultivate, the condition of whole Primary culture is light intensity 1000-2000Lux, light application time 12 hours/day, temperature is between 23-26 DEG C, (2) proliferation-inducing of axillalry bud, after axillalry bud grows tall into the seedling of more than 4 centimetres, be cut into the stem section of 2 centimeter length, every length of tape 1-3 axil, then oblique cutting is on shoot proliferation medium, axil upward, ensure that axil place can obtain sufficient illumination, proliferated culture medium is MS+ZT 1.0-1.5mg/L+IBA0.05-0.1mg/L, after 15 days, induce axillalry bud, 1-3 indefinite bud seedling can be induced at axillalry bud base portion simultaneously, again through the cultivation of 30 days, seedling can grow to 4 centimetres high, the stem section again seedling being cut into 2. centimetres of long band 1-3 axils afterwards forwards on identical shoot proliferation medium, through the cultivation of 45 days, the seedling derived can grow tall again 4-5 centimetre, the stem the section repeatedly seedling of high 4-5 centimetre being cut into 2 centimetres of long band 1-3 axils carries out Multiplying culture on proliferated culture medium, its proliferation times is 3-4 times, the condition that whole shoot proliferation is cultivated is light intensity 1000-2000Lux, light application time 12 hours/day, temperature is between 23-26 DEG C, (3) taking root without seed Rosa roxburghii plantlet in vitro, by growing tall, the seedling of 4 centimetres is transferred on root media, root media is 1/2MS+IBA0.01-0.05mg/L+IAA0.01-0.05mg/L, can take root after 15 days, obtain taking root seedling without seed Rosa roxburghii, the condition of whole culture of rootage is light intensity 1000-2000Lux, light application time 12 hours/day, and temperature is between 23-26 DEG C, (4) to take root the cleaning of seedling and transplanting without seed Rosa roxburghii: when the root of seedling of taking root without seed Rosa roxburghii grows to after more than 2 centimetres, direct taking-up is taken root seedling, clean the medium attached at root, carry out acclimatization and transplants afterwards, transplanting medium proportioning is humus soil by volume: perlite=5: 1, notice after transplanting that canopy internal control temperature control is wet, temperature controls between 25-30 degree, humidity is more than 80%, survive after 15 days, move to after surviving in the nutritious bag of 10cm × 10cm, transplanting medium is humus soil by volume: perlite=5: 1.
This method and technology without seed Rosa roxburghii seedling fast-propagation is complicated, be difficult to grasp, and cost is high, is not suitable for actual applying.In addition, this technology for be without seed Rosa roxburghii, instead of Rosa roxburghii (note: be different two species without seed Rosa roxburghii and Rosa roxburghii).
Cultivation method without seed Rosa roxburghii: the selection on ground, (1) seedbed: select soil layer deep, better organic, water condition is convenient, the not low-lying soil causing flood; (2) open railway carriage or compartment: by face, 1.2M railway carriage or compartment trench digging, the dark 32cm of furrow, soil in small, broken bits, mu applies well-rotted farmyard manure 1500 kilograms or composite fertilizer 120kg, and seed bed preparation terminates wholely, ground, seedbed cuttage the previous day disposable water permeable; (3) get fringe bar: adopt the healthy branch of winter pruning to do cuttings resource or build maternal plant garden and do the supply of fringe bar, plant 1 year without seed Rosa roxburghii fruit tree, base portion sprouting 4-5 lopwood bar, growing way is vigorous, a large amount of branch can do tloig-cutlage, after removing fringe, cuts fringe stay bud to fringe bar, every fringe saves 2 buds, after guaranteeing at least cuttage, a bud is impaired, and another bud survives, and fringe joint configures APD root-inducing powder 50ppm solution leaching root before cuttage, base portion will be inserted to immerse in solution 5 minutes, after leaching, place 4-8h cuttage again; (4) cuttage: cuttage can adopt seedbed soil cuttage and the cuttage of this alms bowl of nutrition soil, and seedbed cutting density presses the square cuttage of 3cm × 3cm, and every mu of cave Basic Seedling reaches 60,000 strains; Nutritive cube device each cuttage one strain, nutrition pot seedling can be transplanted in advance, 5 months just portables after cutting survival, and seedbed cuttage terminates rear bamboo chip arch camber epiphragma, and film edge soil is tight, stuffy ventilative, keeps film humidity; (5) manage: cuttage is carried out winter, to March in spring after Survival rate of nursery stock, temperature bottom out, as occurred, more than temperature 28 DEG C degree high temperature will notice that taking off film ventilates, and starts hardening simultaneously; At the beginning of 4 months, temperature obviously raises, and does not need to keep high temperature in film, can open film artificial weeding, fertilizing, watering, the available clear liquid dung that applies fertilizer is executed and is watered, and available every 100kg water is converted 1.2kg urea and imposed to autumn, topdress 2-3 time, nursery soil now white lack of water of watering, notes observing and waters in good time; (6) go out garden: by 1 year seedbed time growth management, one year seedling, up to 45-50cm, just can go out garden and transplant.
This cultivation method without seed Rosa roxburghii directly uses branch cutting to afforest, and is applicable to the environment that the condition such as soil, moisture is very superior.And the conditions such as Karst Rocky Desertification environment soil layer is very thin, soil depletion, moisture are all very severe, this technology cannot be suitable for.
Summary of the invention
Lack based on the Guizhou mountainous area current nursery of medicine-food two-purpose Special plant Rosa roxburghii and culture technique, prior art is complicated, be difficult to grasp, cost is high, is not suitable for actual in Karst rocky desertification applying.The invention provides one to may be used for quick, easy, efficient Rosa roxburghii (Rosa roxburghii) isolated shoot seedling-cultivating method and be applied to the afforestation technology in Rocky Desertification Control.Simple and effective of the present invention, is suitable for Rosa roxburghii Fast-propagation and is applied to the production practices such as Rocky Desertification Control, not only can produce obvious economic benefit, also have the good ecological benefits such as water and soil conservation, revegetation simultaneously.
The technical solution used in the present invention is as follows:
A kind of Rosa roxburghii isolated shoot seedling-cultivating method, comprises the steps:
Step (1), the selection of female branch and process: life in 2 years of growth selection stalwartness and perennial Rosa roxburghii branch, as female branch of nursery, are cut into the stem section of 15 ~ 20 centimeter length, every length of tape 1-3 bud point;
Step (2), nursery: the stem section obtained of step (1) is wrapped lower end with the moistening absorbent material of the heteroauxin solution being soaked with 50mg/L, then place it in culture plate or cultivate in cup, again culture plate or cultivation cup are put into light transmission container, room temperature is placed, and keeps the skin wet every 1 ~ 2 day, keeps the gauze of branch lower end parcel moistening, after 15 days, the stem section more than 90% roots becomes a complete seedling;
Step (3), transplant hardening: seedling replanting step (2) obtained is in nutritious bag, transplanting medium is humus soil by volume: perlite=4: 1 ~ 5: 1, shelter from heat or light and control 40% ~ 60%, temperature controls at 20 ~ 30 DEG C, cultivate after 30 days, obtain robust growth, Rosa roxburghii seedling that height of seedling reaches more than 30cm.
Further, preferably the absorbent material described in step (2) is cotton, filter paper or cotton gauze.
Further, preferably the specification of the nutritious bag described in step (3) is 10cm × 10cm ~ 12cm × 12cm.
The robust growth that above-mentioned Rosa roxburghii isolated shoot seedling-cultivating method obtains, height of seedling reach the application of Rosa roxburghii seedling in Rocky Desertification Control of more than 30cm.
The robust growth that above-mentioned seedling-cultivating method obtains, height of seedling reach the application of Rosa roxburghii seedling in Rocky Desertification Control of more than 30cm, and its method of forestation is as follows:
Step (1), planting density, whole ground specification and method: planting density is 300cm × 300cm; Land preparation mode is cave-shaped, and plant pit specification is footpath, cave 40cm × 30cm; Require to carry out along contour wholely, during whole ground kind plant hole by " product, font configures, and simultaneously the thick dry branches and fallen leaves of one deck 3 ~ 5cm and fertilizer are spread in every cave, reduce the underground leakage of moisture, improve water retention capacity, increase ground temperature;
Step (2), mode of planting seedlings and time: inspect for acceptance for the whole good ground of step (1), after qualified, selects rainy weather to plant seedlings again; When planting seedlings, first root is hindered in finishing, plants seedlings and selects in February in early spring to March, by the method for " three to fill out, two to step on, carry seedling ", needs to water sufficient normal root water simultaneously;
Step (3), fertilising: before planting seedlings, use sufficient base fertilizer, use sufficient by 15kg/ strain farmyard manure as base manure, after transplanted seedling survives, by 3.3kg/ mu urea as carrying seed manure for executing;
Step (4), after-culture is mended and is made: to the bottom class of survival rate below 85%, carrying out after-culture in time winter then.
Further, preferably stony desertification is seriously regional, does not limit during prepare for afforestation by seeding row spacing.
Further, when preferably step (2) is planted seedlings, after root is hindered in finishing, to immerse in mud or water 3 ~ 5 minutes.
Seedling replanting of the present invention not only can adopt nutritious bag, other containers with similar effect or function all can, as simple and easy materials such as disposal plastic cups.
Culture plate or cultivation cup are put into light transmission container by the present invention, main consideration can be added a cover, reduce evaporation, prevent moisture content from losing fast, but also can not put into light transmission container, directly there is the evaporation of the moisture that slow down such as the lid of air-vent, if when high humidity at culture plate or cultivation cup upper cover, this is not then needed to operate, depending on actual conditions.
Compared with prior art, its beneficial effect is in the present invention:
(1) the present invention is simple and easy to do, and cost is low, without soil pollution, efficiently, be suitable for Rosa roxburghii Fast-propagation and be applied to the production practices such as Rocky Desertification Control, not only can produce obvious economic benefit, also there are the good ecological benefits such as water and soil conservation, revegetation simultaneously.
(2) transplanting survival rate of the present invention reaches more than 95%, and application Rocky Desertification Control has a significant effect, and administer district's vegetation coverage and improve 6 ~ 8%, soil erosion modulus reduces by 15 ~ 20%, and stony desertification area ratio reduces by 10 ~ 15%.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
It will be understood to those of skill in the art that the following example only for illustration of the present invention, and should not be considered as limiting scope of the present invention.Unreceipted concrete technology or condition person in embodiment, according to the technology described by the document in this area or condition or carry out according to product description.Agents useful for same or the unreceipted production firm person of instrument, being can by buying the conventional products obtained.
Embodiment 1
A kind of Rosa roxburghii isolated shoot seedling-cultivating method, comprises the steps:
Step (1), the selection of female branch and process: life in 2 years of growth selection stalwartness and perennial Rosa roxburghii branch, as female branch of nursery, are cut into the stem section of 15 centimeter length, every length of tape 1 bud point;
Step (2), nursery: the stem section obtained of step (1) is wrapped lower end with the moistening absorbent material of the heteroauxin solution being soaked with 50mg/L, then place it in and cultivate in cup, again culture plate or cultivation cup are put into light transmission container, room temperature is placed, and keeps the skin wet every 1 day, keeps the gauze of branch lower end parcel moistening, after 15 days, the stem section more than 90% roots becomes a complete seedling; Described absorbent material is cotton, filter paper or cotton gauze;
Step (3), transplant hardening: seedling replanting step (2) obtained is in nutritious bag, transplanting medium is humus soil by volume: perlite=4: 1, shelter from heat or light and control 40% ~ 45%, temperature controls at 20 DEG C, cultivate after 30 days, obtain robust growth, Rosa roxburghii seedling that height of seedling reaches more than 30cm; The specification of described nutritious bag is 10cm × 10cm.
The robust growth that the present embodiment seedling-cultivating method obtains, height of seedling reach the application of Rosa roxburghii seedling in Rocky Desertification Control of more than 30cm, and its method of forestation is as follows:
Step (1), planting density, whole ground specification and method: planting density is 300cm × 300cm; Land preparation mode is cave-shaped, and plant pit specification is footpath, cave 40cm × 30cm; Require to carry out along contour, during whole ground, kind plant hole is by triangle disposition configuration wholely, and the thick dry branches and fallen leaves of one deck 3cm and fertilizer are spread in every cave simultaneously, reduce the underground leakage of moisture, improve water retention capacity, increase ground temperature;
Step (2), mode of planting seedlings and time: inspect for acceptance for the whole good ground of step (1), after qualified, selects rainy weather to plant seedlings again; When planting seedlings, first root is hindered in finishing, plants seedlings and selects in February in early spring to March, by the method for " three to fill out, two to step on, carry seedling ", needs to water sufficient normal root water simultaneously;
Step (3), fertilising: before planting seedlings, use sufficient base fertilizer, use sufficient by 15kg/ strain farmyard manure as base manure, after transplanted seedling survives, by 3.3kg/ mu urea as carrying seed manure for executing;
Step (4), after-culture is mended and is made: to the bottom class of survival rate below 85%, carrying out after-culture in time winter then.
The present embodiment transplanting survival rate reaches 95.5%, and application Rocky Desertification Control has a significant effect, and administer district's vegetation coverage and improve 6.2%, soil erosion modulus reduces by 18%, and stony desertification area ratio reduces by 13%.
Embodiment 2
A kind of Rosa roxburghii isolated shoot seedling-cultivating method, comprises the steps:
Step (1), the selection of female branch and process: life in 2 years of growth selection stalwartness and perennial Rosa roxburghii branch, as female branch of nursery, are cut into the stem section of 20 centimeter length, every length of tape 3 bud points;
Step (2), nursery: the stem section obtained of step (1) is wrapped lower end with the moistening absorbent material of the heteroauxin solution being soaked with 50mg/L, then place it in culture plate, again culture plate or cultivation cup are put into light transmission container, room temperature is placed, and keeps the skin wet every 2 days, keeps the gauze of branch lower end parcel moistening, after 15 days, the stem section more than 90% roots becomes a complete seedling; Described absorbent material is cotton, filter paper or cotton gauze;
Step (3), transplant hardening: seedling replanting step (2) obtained is in nutritious bag, transplanting medium is humus soil by volume: perlite=5: 1, shelter from heat or light and control 55% ~ 60%, temperature controls at 30 DEG C, cultivate after 30 days, obtain robust growth, Rosa roxburghii seedling that height of seedling reaches more than 30cm; The specification of described nutritious bag is 12cm × 12cm.
The robust growth that the present embodiment seedling-cultivating method obtains, height of seedling reach the application of Rosa roxburghii seedling in Rocky Desertification Control of more than 30cm, and its method of forestation is as follows:
Step (1), planting density, whole ground specification and method: planting density is 300cm × 300cm; Land preparation mode is cave-shaped, and plant pit specification is footpath, cave 40cm × 30cm; Require to carry out along contour, during whole ground, kind plant hole is by triangle disposition configuration wholely, and the thick dry branches and fallen leaves of one deck 5cm and fertilizer are spread in every cave simultaneously, reduce the underground leakage of moisture, improve water retention capacity, increase ground temperature;
Step (2), mode of planting seedlings and time: inspect for acceptance for the whole good ground of step (1), after qualified, selects rainy weather to plant seedlings again; When planting seedlings, first root is hindered in finishing, is then immersed in the water 3 minutes by hindering root, plants seedlings and selects at early March in early spring, by the method for " three to fill out, two to step on, carry seedling ", needs to water sufficient normal root water simultaneously;
Step (3), fertilising: before planting seedlings, use sufficient base fertilizer, use sufficient by 15kg/ strain farmyard manure as base manure, after transplanted seedling survives, by 3.3kg/ mu urea as carrying seed manure for executing;
Step (4), after-culture is mended and is made: to the bottom class of survival rate below 85%, carrying out after-culture in time winter then.
The present embodiment transplanting survival rate reaches 97%, and application Rocky Desertification Control has a significant effect, and administer district's vegetation coverage and improve 7.2%, soil erosion modulus reduces by 19.5%, and stony desertification area ratio reduces by 15%.
Embodiment 3
A kind of Rosa roxburghii isolated shoot seedling-cultivating method, comprises the steps:
Step (1), the selection of female branch and process: life in 2 years of growth selection stalwartness and perennial Rosa roxburghii branch, as female branch of nursery, are cut into the stem section of 18 centimeter length, every length of tape 2 bud points;
Step (2), nursery: the stem section obtained of step (1) is wrapped lower end with the moistening absorbent material of the heteroauxin solution being soaked with 50mg/L, then place it in culture plate, again culture plate or cultivation cup are put into light transmission container, room temperature is placed, and keeps the skin wet every 1.3 days, keeps the gauze of branch lower end parcel moistening, after 15 days, the stem section more than 90% roots becomes a complete seedling; Described absorbent material is cotton, filter paper or cotton gauze;
Step (3), transplant hardening: seedling replanting step (2) obtained is in nutritious bag, transplanting medium is humus soil by volume: perlite=4.2: 1, shelter from heat or light and control 50% ~ 55%, temperature controls at 24 ~ 25 DEG C, cultivate after 30 days, obtain robust growth, Rosa roxburghii seedling that height of seedling reaches more than 30cm; The specification of described nutritious bag is 11cm × 11cm.
The robust growth that the present embodiment seedling-cultivating method obtains, height of seedling reach the application of Rosa roxburghii seedling in Rocky Desertification Control of more than 30cm, and its method of forestation is as follows:
Step (1), planting density, whole ground specification and method: planting density is 300cm × 300cm; Land preparation mode is cave-shaped, and plant pit specification is footpath, cave 40cm × 30cm; Require to carry out along contour, during whole ground, kind plant hole is by triangle disposition configuration wholely, and the thick dry branches and fallen leaves of one deck 3.7cm and fertilizer are spread in every cave simultaneously, reduce the underground leakage of moisture, improve water retention capacity, increase ground temperature;
Step (2), mode of planting seedlings and time: inspect for acceptance for the whole good ground of step (1), after qualified, selects rainy weather to plant seedlings again; When planting seedlings, first root is hindered in finishing, after root is hindered in finishing, to immerse in mud 5 minutes, plant seedlings and select in February in early spring, by the method for " three to fill out, two to step on, carry seedling ", needs to water sufficient normal root water simultaneously;
Step (3), fertilising: before planting seedlings, use sufficient base fertilizer, use sufficient by 15kg/ strain farmyard manure as base manure, after transplanted seedling survives, by 3.3kg/ mu urea as carrying seed manure for executing;
Step (4), after-culture is mended and is made: to the bottom class of survival rate below 85%, carrying out after-culture in time winter then.
The present embodiment transplanting survival rate reaches 99%, and application Rocky Desertification Control has a significant effect, and administer district's vegetation coverage and improve 8%, soil erosion modulus reduces by 19.6%, and stony desertification area ratio reduces by 14.2%.
Embodiment 4
A kind of Rosa roxburghii isolated shoot seedling-cultivating method, comprises the steps:
Step (1), the selection of female branch and process: life in 2 years of growth selection stalwartness and perennial Rosa roxburghii branch, as female branch of nursery, are cut into the stem section of 16-17 centimeter length, every length of tape 2-3 bud point;
Step (2), nursery: the stem section obtained of step (1) is wrapped lower end with the moistening absorbent material of the heteroauxin solution being soaked with 50mg/L, then place it in and cultivate in cup, again culture plate or cultivation cup are put into light transmission container, room temperature is placed, and keeps the skin wet every 1.8 days, keeps the gauze of branch lower end parcel moistening, after 15 days, the stem section more than 90% roots becomes a complete seedling; Described absorbent material is cotton, filter paper or cotton gauze;
Step (3), transplant hardening: seedling replanting step (2) obtained is in nutritious bag, transplanting medium is humus soil by volume: perlite=4.5: 1, shelter from heat or light and control 48% ~ 50%, temperature controls at 26 ~ 28 DEG C, cultivate after 30 days, obtain robust growth, Rosa roxburghii seedling that height of seedling reaches more than 30cm; The specification of described nutritious bag is 10cm × 10cm.
The robust growth that the present embodiment seedling-cultivating method obtains, height of seedling reach the application of Rosa roxburghii seedling in Rocky Desertification Control of more than 30cm, and its method of forestation is as follows:
Step (1), planting density, whole ground specification and method: stony desertification is seriously regional, does not limit during prepare for afforestation by seeding row spacing; Land preparation mode is cave-shaped, and plant pit specification is footpath, cave 40cm × 30cm; Require to carry out along contour, during whole ground, kind plant hole is by triangle disposition configuration wholely, and the thick dry branches and fallen leaves of one deck 4.5cm and fertilizer are spread in every cave simultaneously, reduce the underground leakage of moisture, improve water retention capacity, increase ground temperature;
Step (2), mode of planting seedlings and time: inspect for acceptance for the whole good ground of step (1), after qualified, selects rainy weather to plant seedlings again; When planting seedlings, first root is hindered in finishing, after root is hindered in finishing, to immerse in mud 4 minutes, plant seedlings and select in mid-March in early spring, by the method for " three to fill out, two to step on, carry seedling ", needs to water sufficient normal root water simultaneously;
Step (3), fertilising: before planting seedlings, use sufficient base fertilizer, use sufficient by 15kg/ strain farmyard manure as base manure, after transplanted seedling survives, by 3.3kg/ mu urea as carrying seed manure for executing;
Step (4), after-culture is mended and is made: to the bottom class of survival rate below 85%, carrying out after-culture in time winter then.
The present embodiment transplanting survival rate reaches 99.5%, and application Rocky Desertification Control has a significant effect, and administer district's vegetation coverage and improve 6.3%, soil erosion modulus reduces by 15.5%, and stony desertification area ratio reduces by 10%.
More than show and describe general principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (7)
1. a Rosa roxburghii isolated shoot seedling-cultivating method, is characterized in that, comprises the steps:
Step (1), the selection of female branch and process: life in 2 years of growth selection stalwartness and perennial Rosa roxburghii branch, as female branch of nursery, are cut into the stem section of 15 ~ 20 centimeter length, every length of tape 1-3 bud point;
Step (2), nursery: the stem section obtained of step (1) is wrapped lower end with the moistening absorbent material of the heteroauxin solution being soaked with 50mg/L, then place it in culture plate or cultivate in cup, room temperature is placed, kept the skin wet every 1 ~ 2 day, keep the gauze of branch lower end parcel moistening, after 15 days, the stem section more than 90% roots becomes a complete seedling;
Step (3), transplant hardening: seedling replanting step (2) obtained is in nutritious bag, transplanting medium is humus soil by volume: perlite=4: 1 ~ 5: 1, shelter from heat or light and control 40% ~ 60%, temperature controls at 20 ~ 30 DEG C, cultivate after 30 days, obtain robust growth, Rosa roxburghii seedling that height of seedling reaches more than 30cm.
2. Rosa roxburghii isolated shoot seedling-cultivating method according to claim 1, is characterized in that the absorbent material described in step (2) is cotton, filter paper or cotton gauze.
3. Rosa roxburghii isolated shoot seedling-cultivating method according to claim 1, is characterized in that the specification of the nutritious bag described in step (3) is 10cm × 10cm ~ 12cm × 12cm.
4. the robust growth obtained based on the Rosa roxburghii isolated shoot seedling-cultivating method described in claim 1 or 2 or 3, height of seedling reach the application of Rosa roxburghii seedling in Rocky Desertification Control of more than 30cm.
5. Rosa roxburghii isolated shoot seedling-cultivating method according to claim 4 obtains robust growth, height of seedling reach the application of Rosa roxburghii seedling in Rocky Desertification Control of more than 30cm, and it is characterized in that, method of forestation is as follows:
Step (1), planting density, whole ground specification and method: planting density is 300cm × 300cm; Land preparation mode is cave-shaped, and plant pit specification is footpath, cave 40cm × 30cm; Require to carry out along contour, during whole ground, kind plant hole is by triangle disposition configuration wholely, and the thick dry branches and fallen leaves of one deck 3 ~ 5cm and fertilizer are spread in every cave simultaneously;
Step (2), mode of planting seedlings and time: inspect for acceptance for the whole good ground of step (1), after qualified, selects rainy weather to plant seedlings again; When planting seedlings, first root is hindered in finishing, plants seedlings and selects in February in early spring to March, by the method for " three to fill out, two to step on, carry seedling ", needs to water sufficient normal root water simultaneously;
Step (3), fertilising: before planting seedlings, use sufficient base fertilizer, use sufficient by 15kg/ strain farmyard manure as base manure, after transplanted seedling survives, by 3.3kg/ mu urea as carrying seed manure for executing;
Step (4), after-culture is mended and is made: to the bottom class of survival rate below 85%, carrying out after-culture in time winter then.
6. Rosa roxburghii isolated shoot seedling-cultivating method according to claim 5 obtains robust growth, height of seedling reach the application of Rosa roxburghii seedling in Rocky Desertification Control of more than 30cm, and it is characterized in that, stony desertification is seriously regional, does not limit during prepare for afforestation by seeding row spacing.
7. Rosa roxburghii isolated shoot seedling-cultivating method according to claim 5 obtains robust growth, height of seedling reach the application of Rosa roxburghii seedling in Rocky Desertification Control of more than 30cm, it is characterized in that, when step (2) is planted seedlings, after root is hindered in finishing, to immerse in mud or water 3 ~ 5 minutes.
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