CN103548652A - Rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings - Google Patents
Rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings Download PDFInfo
- Publication number
- CN103548652A CN103548652A CN201310509213.2A CN201310509213A CN103548652A CN 103548652 A CN103548652 A CN 103548652A CN 201310509213 A CN201310509213 A CN 201310509213A CN 103548652 A CN103548652 A CN 103548652A
- Authority
- CN
- China
- Prior art keywords
- seedling
- water
- bondage
- sodium
- field planting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y02P60/216—
Landscapes
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings. The rapid tree cultivation method is to bind dendrobium candidum tissue-culture bottle seedlings after subjected to short-term refining on threes for cultivation, and includes seedling hardening, seedling fetching, pretreatment of seedlings, treatment on tree bark and moss, preparation of a binding material, binding of the seedlings, spraying sanitization, spraying management and final-period management. According to the invention, the tree cultivation method is simplified, seedling hardening and tree cultivation effects are optimized, production period is shortened, relatively high seedling survival rate is guaranteed as the survival rate is above 0.095, the obtained medicine is high in quality, multiple effects of speediness, high yield and excellent production are achieved, and the rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings is an efficient and feasible tree cultivation method for dendrobium candidum.
Description
Technical field
The present invention relates to biological technical field, relate in particular to a kind of quick tree culture method of candidum tissue culturing bottle seedling.
Background technology
Dendrobium candidum is one of most precious traditional Chinese medicine of China, is classified as first of " Chinese nine immortal grass " by honor, have the laudatory title of " gold in medicine ", and international medicinal plant circle is called “Yao circle giant panda ".The main medicinal ingredient of dendrobium candidum has the trace element of polysaccharide, flavones, alkaloid, amino acid, phenols and tens kinds of human body requirements etc.The iron-sheet dendrobe fresh product of per kilogram can be sold for more than thousand yuan at present.The imitative wild artificial planting stem of noble dendrobium, the stem of noble dendrobium effective component obtaining than greenhouse gardening is higher, and the market price also exceeds more than three times than common dendrobium candidum.Because plantation is on trunk, upper and lower three-dimensional plantation has improved output.Yet dendrobium candidum seedling group training seedling need to be through more than booth domestication half a year, front " climbing the tree ", also needs the sylvan life vegetative period through four to six months, can be transplanted to and on tree, carry out imitating wild planting.In prior art, common way is in annual May～November, and dendrobium candidum seedling, first by container nursery, is planted in the shade net of booth, tides over domestication; After six months, duration November～March, start to move on to sylvan life, select again afterwards the evergreen broad-leaved forest at shady and cool, moistening place or have the plantation ' place ' of liver moss.Strengthened so undoubtedly the input of human resources and cost.
CN102499038A discloses a kind of tree culture method of dendrobium candidum, is that the veined seeds in selection bark surface are the tree that grows nonparasitically upon another plant, and selecting the dendrobium candidum seedling through taming is the seedling of growing nonparasitically upon another plant, and selection straw rope is tied rope; The trunk surface that the seedling of growing nonparasitically upon another plant is placed in to the tree that grows nonparasitically upon another plant, is wound around a few at the root place of the seedling of growing nonparasitically upon another plant by tied rope and is fixed; Tree training humidity is controlled at 70-85%.The problem that this scheme solves is to realize planting dendrobium candidum on the veined seeds of all surface, and its deficiency is that between seedling, line-spacing is unreasonable, and spacing in the rows is not clear, has wasted plantation space; Before seedling is climbed the tree, lack pretreatment, the measure deficiency of climbing the tree, causes the growth of dendrobium candidum seedling comparatively slow, and the quality of the medicinal material of acquisition is not ideal enough.
And for example the disclosed a kind of dendrobium candidum of CN102823401A is tied up tree cultivation method, be=comprise that seedling group training breeds, training tissue culture seedling, domestication seedling is tied up tree cultivation, its Special attention will be given to be hardening matrix, hardening fertilizer, domestication seedling, cultivation forest land, tie up the selection of tree seeds, and the method for tying up seedling (is attached to the moss of cleaning and sterilized the root of domestication seedling, with the rope made of hemp of sterilizing or Cortex Cunninghamiae Lanceolatae, domestication seedling is tied up on trunk, the rope made of hemp or Cortex Cunninghamiae Lanceolatae are tied up the root at domestication seedling, every 4～7 strain domestication seedlings are one clump), in cultivation management method, emphasize water spray opportunity and ambient humidity requirement.The deficiency of this scheme is, the domestication time of seedling is grown (18～30 months), and the production cycle is long, and operating process is comparatively complicated.
Summary of the invention
The object of the present invention is to provide a kind of quick tree culture method of candidum tissue culturing bottle seedling, solved in prior art that the dendrobium candidum tree culture method domestication time is long, the production cycle is grown, yield poorly, measure deficiency causes the undesirable problem of medical material quanlity.
A quick tree culture method for bottle seedling, is that candidum tissue culturing bottle seedling is cultivated to tree is upper by bondage after refinery, comprises the following steps:
(1) hardening and get seedling:
To in candidum tissue culturing bottle Miao Zhengpingcong culturing room, move to glass sunlight house hardening after 10～20 days, bottle cap is unscrewed and placed an evening, second day is opened bottle cap, with tweezers, from bottle, seedling is pressed from both sides out, and rinsing is clean, pulls out and drains 24～48h;
(2) pretreatment of seedling:
Treating that seedling that step (1) gained drains is sterilized and dried in the air to root is white in color, and shoot root portion is put to field planting water and soaks 25～35min, then pulls out, drains away the water;
Described field planting water constituent is: Sodium p-nitrophenoxide 0.8 ‰～1.2 ‰, sodium onitrophenol 1.8 ‰～2.2 ‰, 5-nitroguaiacol sodium salt 1.8 ‰～2.2 ‰, thiamine hydrochloride 99 ‰～101 ‰, puridoxine hydrochloride 4.8 ‰～5.2 ‰, inositol 99 ‰～101 ‰, and all the other are water;
(3) processing of bark and liver moss:
Bark superficial is suitably processed, removed impurity and branch, sterilize standby, after liver moss is just returned, clean up, dry, put into thimerosal and soak 5～10min, pull out, firmly squeezing, falls apart to be open in clean plane and dries, standby;
(4) preparation of binding material:
Sunshade net is cut into the binding material of wide 6～8cm, long 3～4m;
(5) bondage of seedling:
With liver moss, encase dendrobium candidum shoot root portion one side, expose foundation portion, the opposite side that liver moss is not encased is affixed to bark superficial, spacing in the rows 4～6cm, with the binding material in step (4), carry out bondage again, after 2 circles, tie, then successively bondage, line-spacing 15～20cm, the bondage on every tree is highly 4～6m;
(6) spraying disinfection, spray management:
In 48h after bondage completes, carry out spraying disinfection once, every 4～6 days, spray one time field planting water;
Described field planting water constituent is: Sodium p-nitrophenoxide 0.8 ‰～1.2 ‰, sodium onitrophenol 1.8 ‰～2.2 ‰, 5-nitroguaiacol sodium salt 1.8 ‰～2.2 ‰, thiamine hydrochloride 99 ‰～101 ‰, puridoxine hydrochloride 4.8 ‰～5.2 ‰, inositol 99 ‰～101 ‰, and all the other are water;
(7) final-period management:
Final-period management operates routinely, prevention and elimination of disease and pests, and humidity adjustment is carried out according to a conventional method.
The composition of the water of field planting described in the present invention is: Sodium p-nitrophenoxide 0.9 ‰～1.1 ‰, sodium onitrophenol 1.9 ‰～2.1 ‰, 5-nitroguaiacol sodium salt 1.9 ‰～2.1 ‰, thiamine hydrochloride 99.5 ‰～100.5 ‰, puridoxine hydrochloride 4.9 ‰～5.1 ‰, inositol 99.5 ‰～100.5 ‰, all the other are water.
The composition of the water of field planting described in the present invention is: Sodium p-nitrophenoxide 1.0 ‰, sodium onitrophenol 2.0 ‰, 5-nitroguaiacol sodium salt 2.0 ‰, thiamine hydrochloride 100.0 ‰, puridoxine hydrochloride 5.0 ‰, inositol 100.0 ‰, all the other are water.
The hardening time in step of the present invention (1) is 14～16 days.
Step of the present invention is put shoot root portion to field planting water and is soaked 29～31min in (2).
In step of the present invention (3), liver moss is put into thimerosal and soak 7～8min.
In step of the present invention (5), the spacing in the rows of dendrobium candidum seedling is 5cm, and line-spacing is 17～18cm.
In step of the present invention (4), sunshade net is cut into the binding material of wide 7cm, long 3.5m.
Beneficial effect of the present invention: simplified tree culture method, optimized hardening and tree training effect, shortened the production cycle, guaranteed higher seedling survival rate, the medical material quanlity obtaining is superior, reached fast, the multiple effect of high yield, excellent product, and be a kind of dendrobium candidum tree culture method of efficient easily row.The present invention coordinates specific seedling processing method by glass sunlight house short time hardening, plants and compares again half a year with booth domestication conventional in prior art, can greatly shorten the dendrobium candidum seedling domestication time to external world; By specific bondage technology, coordinate the use of field planting water, can improve the survival rate of dendrobium candidum seedling, the manpower, the financial resources that significantly reduce dendrobium candidum plantation drop into, its cost is about 1/3rd left and right of traditional growing dendrobium cost, and easy operating, generalization is strong, to driving medicinal herb grower's economic development to do a great deal of good.Adopt the inventive method plantation candidum tissue culturing bottle seedling, survival rate reaches more than 95 ‰; Greatly improve dendrobium candidum output, shortened the production cycle; Dendrobium candidum annual value of production on every tree can tens thousand of units, and the flower of dendrobium candidum (the making tea bubble drink) benefit that also can produce 2～30,000 yuan per jin; The final dendrobium candidum plant pedicel obtaining is full, black joint is obvious, and there is formae oxydatae on surface, diameter 0.6～0.8cm, be about 15～20cm, frangibility, section is colloid, and raw chewing glued mouth, chew rear few slag, fresh goods functional component is high, has retained to greatest extent the competent cell of dendrobium candidum, tool green health health effect; Made golden yellow, glossy, the thin longitudinal grin of tool in dry product medicinal material surface, quality is pliable and tough and real, and section is more smooth, and quality is superior.
The present invention adopts sunshade net to substitute tied rope, can play good be in the light, keep off the rain, moisturizing, thermoregulatory effect, and light weight, good permeability, long service life, is better than common tied rope.The present invention adopts pretreated liver moss and bark to be more suitable for dendrobium candidum seedling rooting, has reduced to a certain extent the probability that viral evil occurs, and thimerosal can be selected the Common Disinfectant Solutions such as carbendazim, tpn liquid.The present invention unscrews bottle cap and places an evening after hardening, and second day is opened bottle cap, can promote the gas exchange that bottle is inside and outside, strengthens the refinery effect of seedling.The present invention uses the field planting water of specific proportioning can obtain beyond thought effect, not only in the shortening refinery time, has improved at the same time the survival rate of dendrobium candidum seedling, has also promoted the quality of dendrobium candidum plant.The present invention is clear and definite is immersed in the time range in field planting water by shoot root portion, can under in order to be effective prerequisite, save the production time, is conducive to enhance productivity, and shortens the production cycle.In the present invention, the width of binding material and length are specific, and spacing in the rows and line-spacing during bondage dendrobium candidum seedling are specific, compared with prior art, greatly saved plantation space, effectively utilize resource, and obtained good culture effect, improved the yield per unit area of dendrobium candidum.The present invention carries out spraying disinfection and spray field planting water after bondage completes, and effectively prevention and elimination of disease and pests is conducive to improve survival rate and the output of dendrobium candidum, is conducive to promote the quality of dendrobium candidum.
Embodiment
Below in conjunction with specific embodiment, technical scheme of the present invention is described.The following stated is preferred embodiment of the present invention, can be used for explaining and supporting the present invention, but the present invention is not done to any pro forma restriction, any technical solution of the present invention content that do not depart from, and according to simple modification, equivalent variations and modification that technical spirit of the present invention is done following examples, all still belong in the scope of technical solution of the present invention.
Embodiment 1
(1) hardening and get seedling:
To in candidum tissue culturing bottle Miao Zhengpingcong culturing room, move to glass sunlight house hardening after 10 days, bottle cap is unscrewed and placed an evening, second day is opened bottle cap, with tweezers, from bottle, seedling is pressed from both sides out, and rinsing is clean, pulls out and drains 24h;
(2) pretreatment of seedling:
Treating that seedling that step (1) gained drains is sterilized and dried in the air to root is white in color, and shoot root portion is put to field planting water and soaks 25min, then pulls out, drains away the water;
Described field planting water constituent is: Sodium p-nitrophenoxide 0.8 ‰, sodium onitrophenol 1.8 ‰, 5-nitroguaiacol sodium salt 1.8 ‰, thiamine hydrochloride 99 ‰, puridoxine hydrochloride 4.8 ‰, inositol 99 ‰, and all the other are water;
(3) processing of bark and liver moss:
Bark superficial is suitably processed, removed impurity and branch, sterilize standby, after liver moss is just returned, clean up, dry, put into thimerosal and soak 5min, pull out, firmly squeezing, falls apart to be open in clean plane and dries, standby;
(4) preparation of binding material:
Sunshade net is cut into the binding material of wide 6cm, long 3m;
(5) bondage of seedling:
With liver moss, encase dendrobium candidum shoot root portion one side, expose foundation portion, the opposite side that liver moss is not encased is affixed to bark superficial, spacing in the rows 4cm, then carry out bondage with the binding material in step (4), after 2 circles, tie, then successively bondage, line-spacing 15cm, the bondage on every tree is highly 4m;
(6) spraying disinfection, spray management:
In 48h after bondage completes, carry out spraying disinfection once, every 4 days, spray one time field planting water;
Described field planting water constituent is: Sodium p-nitrophenoxide 0.8 ‰, sodium onitrophenol 1.8 ‰, 5-nitroguaiacol sodium salt 1.8 ‰, thiamine hydrochloride 99 ‰, puridoxine hydrochloride 4.8 ‰, inositol 99 ‰, and all the other are water;
(7) final-period management:
Final-period management operates routinely, prevention and elimination of disease and pests, and humidity adjustment is carried out according to a conventional method.
Embodiment 2
(1) hardening and get seedling:
To in candidum tissue culturing bottle Miao Zhengpingcong culturing room, move to glass sunlight house hardening after 12 days, bottle cap is unscrewed and placed an evening, second day is opened bottle cap, with tweezers, from bottle, seedling is pressed from both sides out, and rinsing is clean, pulls out and drains 30h;
(2) pretreatment of seedling:
Treating that seedling that step (1) gained drains is sterilized and dried in the air to root is white in color, and shoot root portion is put to field planting water and soaks 27min, then pulls out, drains away the water;
Described field planting water constituent is: Sodium p-nitrophenoxide 1.2 ‰, sodium onitrophenol 2.2 ‰, 5-nitroguaiacol sodium salt 2.2 ‰, thiamine hydrochloride 101 ‰, puridoxine hydrochloride 5.2 ‰, inositol 101 ‰, and all the other are water;
(3) processing of bark and liver moss:
Bark superficial is suitably processed, removed impurity and branch, sterilize standby, after liver moss is just returned, clean up, dry, put into thimerosal and soak 6min, pull out, firmly squeezing, falls apart to be open in clean plane and dries, standby;
(4) preparation of binding material:
Sunshade net is cut into the binding material of wide 6.5cm, long 3.5m;
(5) bondage of seedling:
With liver moss, encase dendrobium candidum shoot root portion one side, expose foundation portion, the opposite side that liver moss is not encased is affixed to bark superficial, spacing in the rows 4.5cm, then carry out bondage with the binding material in step (4), after 2 circles, tie, then successively bondage, line-spacing 16cm, the bondage on every tree is highly 4.5m;
(6) spraying disinfection, spray management:
In 48h after bondage completes, carry out spraying disinfection once, every 5 days, spray one time field planting water;
Described field planting water constituent is: Sodium p-nitrophenoxide 1.2 ‰, sodium onitrophenol 2.2 ‰, 5-nitroguaiacol sodium salt 2.2 ‰, thiamine hydrochloride 101 ‰, puridoxine hydrochloride 5.2 ‰, inositol 101 ‰, and all the other are water;
(7) final-period management:
Final-period management operates routinely, prevention and elimination of disease and pests, and humidity adjustment is carried out according to a conventional method.
Embodiment 3
(1) hardening and get seedling:
To in candidum tissue culturing bottle Miao Zhengpingcong culturing room, move to glass sunlight house hardening after 14 days, bottle cap is unscrewed and placed an evening, second day is opened bottle cap, with tweezers, from bottle, seedling is pressed from both sides out, and rinsing is clean, pulls out and drains 36h;
(2) pretreatment of seedling:
Treating that seedling that step (1) gained drains is sterilized and dried in the air to root is white in color, and shoot root portion is put to field planting water and soaks 29min, then pulls out, drains away the water;
The composition of described field planting water is: Sodium p-nitrophenoxide 0.9 ‰, sodium onitrophenol 1.9 ‰, 5-nitroguaiacol sodium salt 1.9 ‰, thiamine hydrochloride 99.5 ‰, puridoxine hydrochloride 4.9～5.1 ‰, inositol 99.5 ‰, and all the other are water;
(3) processing of bark and liver moss:
Bark superficial is suitably processed, removed impurity and branch, sterilize standby, after liver moss is just returned, clean up, dry, put into thimerosal and soak 7min, pull out, firmly squeezing, falls apart to be open in clean plane and dries, standby;
(4) preparation of binding material:
Sunshade net is cut into the binding material of wide 7cm, long 3.5m;
(5) bondage of seedling:
With liver moss, encase dendrobium candidum shoot root portion one side, expose foundation portion, the opposite side that liver moss is not encased is affixed to bark superficial, spacing in the rows 5cm, then carry out bondage with the binding material in step (4), after 2 circles, tie, then successively bondage, line-spacing 17cm, the bondage on every tree is highly 5m;
(6) spraying disinfection, spray management:
In 48h after bondage completes, carry out spraying disinfection once, every 6 days, spray one time field planting water;
The composition of described field planting water is: Sodium p-nitrophenoxide 0.9 ‰, sodium onitrophenol 1.9 ‰, 5-nitroguaiacol sodium salt 1.9 ‰, thiamine hydrochloride 99.5 ‰, puridoxine hydrochloride 4.9 ‰, inositol 99.5 ‰, and all the other are water;
(7) final-period management:
Final-period management operates routinely, prevention and elimination of disease and pests, and humidity adjustment is carried out according to a conventional method.
Embodiment 4
(1) hardening and get seedling:
To in candidum tissue culturing bottle Miao Zhengpingcong culturing room, move to glass sunlight house hardening after 16 days, bottle cap is unscrewed and placed an evening, second day is opened bottle cap, with tweezers, from bottle, seedling is pressed from both sides out, and rinsing is clean, pulls out and drains 42h;
(2) pretreatment of seedling:
Treating that seedling that step (1) gained drains is sterilized and dried in the air to root is white in color, and shoot root portion is put to field planting water and soaks 31min, then pulls out, drains away the water;
The composition of described field planting water is: Sodium p-nitrophenoxide 1.1 ‰, sodium onitrophenol 2.1 ‰, 5-nitroguaiacol sodium salt 2.1 ‰, thiamine hydrochloride 100.5 ‰, puridoxine hydrochloride 5.1 ‰, inositol 100.5 ‰, and all the other are water;
(3) processing of bark and liver moss:
Bark superficial is suitably processed, removed impurity and branch, sterilize standby, after liver moss is just returned, clean up, dry, put into thimerosal and soak 8min, pull out, firmly squeezing, falls apart to be open in clean plane and dries, standby;
(4) preparation of binding material:
Sunshade net is cut into the binding material of wide 7.5cm, long 3.5m;
(5) bondage of seedling:
With liver moss, encase dendrobium candidum shoot root portion one side, expose foundation portion, the opposite side that liver moss is not encased is affixed to bark superficial, spacing in the rows 5.5cm, then carry out bondage with the binding material in step (4), after 2 circles, tie, then successively bondage, line-spacing 18cm, the bondage on every tree is highly 5.5m;
(6) spraying disinfection, spray management:
In 48h after bondage completes, carry out spraying disinfection once, every 4 days, spray one time field planting water;
The composition of described field planting water is: Sodium p-nitrophenoxide 1.1 ‰, sodium onitrophenol 2.1 ‰, 5-nitroguaiacol sodium salt 2.1 ‰, thiamine hydrochloride 100.5 ‰, puridoxine hydrochloride 5.1 ‰, inositol 100.5 ‰, and all the other are water;
(7) final-period management:
Final-period management operates routinely, prevention and elimination of disease and pests, and humidity adjustment is carried out according to a conventional method.
Embodiment 5
(1) hardening and get seedling:
To in candidum tissue culturing bottle Miao Zhengpingcong culturing room, move to glass sunlight house hardening after 18 days, bottle cap is unscrewed and placed an evening, second day is opened bottle cap, with tweezers, from bottle, seedling is pressed from both sides out, and rinsing is clean, pulls out and drains 48h;
(2) pretreatment of seedling:
Treating that seedling that step (1) gained drains is sterilized and dried in the air to root is white in color, and shoot root portion is put to field planting water and soaks 33min, then pulls out, drains away the water;
The composition of described field planting water is: Sodium p-nitrophenoxide 1.1 ‰, sodium onitrophenol 2.1 ‰, 5-nitroguaiacol sodium salt 2.1 ‰, thiamine hydrochloride 100.5 ‰, puridoxine hydrochloride 5.1 ‰, inositol 100.5 ‰, and all the other are water;
(3) processing of bark and liver moss:
Bark superficial is suitably processed, removed impurity and branch, sterilize standby, after liver moss is just returned, clean up, dry, put into thimerosal and soak 9min, pull out, firmly squeezing, falls apart to be open in clean plane and dries, standby;
(4) preparation of binding material:
Sunshade net is cut into the binding material of wide 8cm, long 4m;
(5) bondage of seedling:
With liver moss, encase dendrobium candidum shoot root portion one side, expose foundation portion, the opposite side that liver moss is not encased is affixed to bark superficial, spacing in the rows 6cm, then carry out bondage with the binding material in step (4), after 2 circles, tie, then successively bondage, line-spacing 19cm, the bondage on every tree is highly 6m;
(6) spraying disinfection, spray management:
In 48h after bondage completes, carry out spraying disinfection once, every 5 days, spray one time field planting water;
The composition of described field planting water is: Sodium p-nitrophenoxide 1.0 ‰, sodium onitrophenol 2.0 ‰, 5-nitroguaiacol sodium salt 2.0 ‰, thiamine hydrochloride 100.0 ‰, puridoxine hydrochloride 5.0 ‰, inositol 100.0 ‰, and all the other are water;
(7) final-period management:
Final-period management operates routinely, prevention and elimination of disease and pests, and humidity adjustment is carried out according to a conventional method.
Embodiment 6
(1) hardening and get seedling:
To in candidum tissue culturing bottle Miao Zhengpingcong culturing room, move to glass sunlight house hardening after 20 days, bottle cap is unscrewed and placed an evening, second day is opened bottle cap, with tweezers, from bottle, seedling is pressed from both sides out, and rinsing is clean, pulls out and drains 36h;
(2) pretreatment of seedling:
Treating that seedling that step (1) gained drains is sterilized and dried in the air to root is white in color, and shoot root portion is put to field planting water and soaks 35min, then pulls out, drains away the water;
The composition of described field planting water is: Sodium p-nitrophenoxide 1.0 ‰, sodium onitrophenol 2.0 ‰, 5-nitroguaiacol sodium salt 2.0 ‰, thiamine hydrochloride 100.0 ‰, puridoxine hydrochloride 5.0 ‰, inositol 100.0 ‰, and all the other are water;
(3) processing of bark and liver moss:
Bark superficial is suitably processed, removed impurity and branch, sterilize standby, after liver moss is just returned, clean up, dry, put into thimerosal and soak 10min, pull out, firmly squeezing, falls apart to be open in clean plane and dries, standby;
(4) preparation of binding material:
Sunshade net is cut into the binding material of wide 7cm, long 4m;
(5) bondage of seedling:
With liver moss, encase dendrobium candidum shoot root portion one side, expose foundation portion, the opposite side that liver moss is not encased is affixed to bark superficial, spacing in the rows 5cm, then carry out bondage with the binding material in step (4), after 2 circles, tie, then successively bondage, line-spacing 20cm, the bondage on every tree is highly 5m;
(6) spraying disinfection, spray management:
In 48h after bondage completes, carry out spraying disinfection once, every 6 days, spray one time field planting water;
The composition of described field planting water is: Sodium p-nitrophenoxide 1.0 ‰, sodium onitrophenol 2.0 ‰, 5-nitroguaiacol sodium salt 2.0 ‰, thiamine hydrochloride 100.0 ‰, puridoxine hydrochloride 5.0 ‰, inositol 100.0 ‰, and all the other are water;
(7) final-period management:
Final-period management operates routinely, prevention and elimination of disease and pests, and humidity adjustment is carried out according to a conventional method.
Claims (8)
1. a quick tree culture method for candidum tissue culturing bottle seedling, is that candidum tissue culturing bottle seedling is cultivated to tree is upper by bondage after refinery, it is characterized in that: comprise the following steps:
(1) hardening and get seedling:
To in candidum tissue culturing bottle Miao Zhengpingcong culturing room, move to glass sunlight house hardening after 10～20 days, bottle cap is unscrewed and placed an evening, second day is opened bottle cap, with tweezers, from bottle, seedling is pressed from both sides out, and rinsing is clean, pulls out and drains 24～48h;
(2) pretreatment of seedling:
Treating that seedling that step (1) gained drains is sterilized and dried in the air to root is white in color, and shoot root portion is put to field planting water and soaks 25～35min, then pulls out, drains away the water;
Described field planting water constituent is: Sodium p-nitrophenoxide 0.8 ‰～1.2 ‰, sodium onitrophenol 1.8 ‰～2.2 ‰, 5-nitroguaiacol sodium salt 1.8 ‰～2.2 ‰, thiamine hydrochloride 99 ‰～101 ‰, puridoxine hydrochloride 4.8 ‰～5.2 ‰, inositol 99 ‰～101 ‰, and all the other are water;
(3) processing of bark and liver moss:
Bark superficial is suitably processed, removed impurity and branch, sterilize standby, after liver moss is just returned, clean up, dry, put into thimerosal and soak 5～10min, pull out, firmly squeezing, falls apart to be open in clean plane and dries, standby;
(4) preparation of binding material:
Sunshade net is cut into the binding material of wide 6～8cm, long 3～4m;
(5) bondage of seedling:
With liver moss, encase dendrobium candidum shoot root portion one side, expose foundation portion, the opposite side that liver moss is not encased is affixed to bark superficial, spacing in the rows 4～6cm, with the binding material in step (4), carry out bondage again, after 2 circles, tie, then successively bondage, line-spacing 15～20cm, the bondage on every tree is highly 4～6m;
(6) spraying disinfection, spray management:
In 48h after bondage completes, carry out spraying disinfection once, every 4～6 days, spray one time field planting water;
Described field planting water constituent is: Sodium p-nitrophenoxide 0.8 ‰～1.2 ‰, sodium onitrophenol 1.8 ‰～2.2 ‰, 5-nitroguaiacol sodium salt 1.8 ‰～2.2 ‰, thiamine hydrochloride 99 ‰～101 ‰, puridoxine hydrochloride 4.8 ‰～5.2 ‰, inositol 99 ‰～101 ‰, and all the other are water;
(7) final-period management:
Final-period management operates routinely, prevention and elimination of disease and pests, and humidity adjustment is carried out according to a conventional method.
2. the quick tree culture method of a kind of candidum tissue culturing bottle seedling as claimed in claim 1, it is characterized in that: the composition of described field planting water is: Sodium p-nitrophenoxide 0.9 ‰～1.1 ‰, sodium onitrophenol 1.9 ‰～2.1 ‰, 5-nitroguaiacol sodium salt 1.9 ‰～2.1 ‰, thiamine hydrochloride 99.5 ‰～100.5 ‰, puridoxine hydrochloride 4.9 ‰～5.1 ‰, inositol 99.5 ‰～100.5 ‰, all the other are water.
3. the quick tree culture method of a kind of candidum tissue culturing bottle seedling as claimed in claim 1, it is characterized in that: the composition of described field planting water is: Sodium p-nitrophenoxide 1.0 ‰, sodium onitrophenol 2.0 ‰, 5-nitroguaiacol sodium salt 2.0 ‰, thiamine hydrochloride 100.0 ‰, puridoxine hydrochloride 5.0 ‰, inositol 100.0 ‰, all the other are water.
4. the quick tree culture method of a kind of candidum tissue culturing bottle seedling as claimed in claim 1, is characterized in that: the hardening time in described step (1) is 14～16 days.
5. the quick tree culture method of a kind of candidum tissue culturing bottle seedling as claimed in claim 1, is characterized in that: described step is put shoot root portion to field planting water and soaked 29～31min in (2).
6. the quick tree culture method of a kind of candidum tissue culturing bottle seedling as claimed in claim 1, is characterized in that: in described step (3), liver moss is put into thimerosal and soak 7～8min.
7. the quick tree culture method of a kind of candidum tissue culturing bottle seedling as claimed in claim 1, is characterized in that: in described step (5), the spacing in the rows of dendrobium candidum seedling is 5cm, and line-spacing is 17～18cm.
8. the quick tree culture method of a kind of candidum tissue culturing bottle seedling as claimed in claim 1, is characterized in that: the binding material that in described step (4), sunshade net is cut into wide 7cm, long 3.5m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310509213.2A CN103548652B (en) | 2013-10-23 | 2013-10-23 | Rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310509213.2A CN103548652B (en) | 2013-10-23 | 2013-10-23 | Rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103548652A true CN103548652A (en) | 2014-02-05 |
| CN103548652B CN103548652B (en) | 2015-06-10 |
Family
ID=50003170
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310509213.2A Expired - Fee Related CN103548652B (en) | 2013-10-23 | 2013-10-23 | Rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103548652B (en) |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104145794A (en) * | 2014-08-15 | 2014-11-19 | 湖南省农业信息与工程研究所 | Temporary planting and seedling hardening method of dendrobium tissue culture seedlings |
| CN104186287A (en) * | 2014-07-30 | 2014-12-10 | 广西壮族自治区药用植物园 | Cultivation method for planting dendrobium candidum seedlings in forest |
| CN104472326A (en) * | 2014-11-06 | 2015-04-01 | 贵州沿河乌江生物科技发展有限公司 | Method for transplanting dendrobium officinale into phyllostachys pubescen |
| CN104782373A (en) * | 2015-04-16 | 2015-07-22 | 戴圣利 | Imitative wild ecology tree planting type cultivation method for dendrobium officinale and cultivation medium manure thereof |
| CN105248266A (en) * | 2015-11-04 | 2016-01-20 | 刘绥 | Planting method of dendrobium officinale on trees |
| CN105532405A (en) * | 2016-01-12 | 2016-05-04 | 云南天泉生物科技股份有限公司 | High-yield cultivation method for tree-grown dendrobium |
| CN105660334A (en) * | 2016-02-01 | 2016-06-15 | 广西顺昌隆生物科技有限公司 | Method for planting potted dendrobium officinale |
| CN105766557A (en) * | 2016-03-15 | 2016-07-20 | 福建省邵武市二都国有林场 | Dendrobium candidum sprinkler irrigation device and cultivation method |
| CN105794612A (en) * | 2016-03-16 | 2016-07-27 | 中国科学院武汉植物园 | Construction method for tropical rainforest hanging garden |
| CN105900667A (en) * | 2016-05-23 | 2016-08-31 | 杨文明 | Method for planting dendrobium candidum in greenhouse |
| CN106550862A (en) * | 2016-11-15 | 2017-04-05 | 广西壮族自治区防城金花茶国家级自然保护区管理处 | The candidum tissue culturing seedling for exempting from seedling exercising ties up tree new method for cultivating |
| CN106613829A (en) * | 2016-11-16 | 2017-05-10 | 陈勇 | Method for planting dendrobium officinale on tree |
| CN108184588A (en) * | 2018-01-16 | 2018-06-22 | 安康市农业科学研究所 | A kind of efficient method for transplanting of konjaku tissue-cultured seedling |
| CN112056197A (en) * | 2020-09-07 | 2020-12-11 | 贵州沿河乌江生物科技发展有限公司 | Rapid tree culture method for dendrobium officinale tissue culture seedlings |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1378781A (en) * | 2001-04-06 | 2002-11-13 | 李智生 | Process for preparing growth promoter of animal and plant |
| CN1711844A (en) * | 2005-07-15 | 2005-12-28 | 中国科学院长春应用化学研究所 | Plant auxin composition for advancing flowering period of kaffir lily |
| CN102282998A (en) * | 2011-06-28 | 2011-12-21 | 云南红土生源药用生物科技开发有限公司 | Dendrobium offcinale tissue cultured seedling training and domesticating method |
| CN102356748A (en) * | 2011-09-23 | 2012-02-22 | 乐清市海上名山铁皮石斛种植专业合作社 | Method for planting dendrobium candidum through imitating wild hanging trees |
| CN102422803A (en) * | 2011-09-21 | 2012-04-25 | 湖南龙石山铁皮石斛基地有限公司 | Three-dimensional original ecological cultivation method for dendrobium officinale in forest |
| CN102499038A (en) * | 2011-10-24 | 2012-06-20 | 浙江雁荡山生态铁皮石斛开发有限公司 | Tree tissue culture method of dendrobium candidum |
| CN102826401A (en) * | 2008-09-18 | 2012-12-19 | 蒋超 | Feeding mechanism and control system thereof |
| CN102986407A (en) * | 2012-10-10 | 2013-03-27 | 肥西县农业技术推广中心 | Dendrobium officinale cultivation method using China fir |
-
2013
- 2013-10-23 CN CN201310509213.2A patent/CN103548652B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1378781A (en) * | 2001-04-06 | 2002-11-13 | 李智生 | Process for preparing growth promoter of animal and plant |
| CN1711844A (en) * | 2005-07-15 | 2005-12-28 | 中国科学院长春应用化学研究所 | Plant auxin composition for advancing flowering period of kaffir lily |
| CN102826401A (en) * | 2008-09-18 | 2012-12-19 | 蒋超 | Feeding mechanism and control system thereof |
| CN102282998A (en) * | 2011-06-28 | 2011-12-21 | 云南红土生源药用生物科技开发有限公司 | Dendrobium offcinale tissue cultured seedling training and domesticating method |
| CN102422803A (en) * | 2011-09-21 | 2012-04-25 | 湖南龙石山铁皮石斛基地有限公司 | Three-dimensional original ecological cultivation method for dendrobium officinale in forest |
| CN102356748A (en) * | 2011-09-23 | 2012-02-22 | 乐清市海上名山铁皮石斛种植专业合作社 | Method for planting dendrobium candidum through imitating wild hanging trees |
| CN102499038A (en) * | 2011-10-24 | 2012-06-20 | 浙江雁荡山生态铁皮石斛开发有限公司 | Tree tissue culture method of dendrobium candidum |
| CN102986407A (en) * | 2012-10-10 | 2013-03-27 | 肥西县农业技术推广中心 | Dendrobium officinale cultivation method using China fir |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104186287A (en) * | 2014-07-30 | 2014-12-10 | 广西壮族自治区药用植物园 | Cultivation method for planting dendrobium candidum seedlings in forest |
| CN104145794A (en) * | 2014-08-15 | 2014-11-19 | 湖南省农业信息与工程研究所 | Temporary planting and seedling hardening method of dendrobium tissue culture seedlings |
| CN104472326A (en) * | 2014-11-06 | 2015-04-01 | 贵州沿河乌江生物科技发展有限公司 | Method for transplanting dendrobium officinale into phyllostachys pubescen |
| CN104472326B (en) * | 2014-11-06 | 2016-08-17 | 贵州沿河乌江生物科技发展有限公司 | A kind of method transplanting Herba Dendrobii on Phyllostachys pubescens Mazei ex H.de Lebaie |
| CN104782373A (en) * | 2015-04-16 | 2015-07-22 | 戴圣利 | Imitative wild ecology tree planting type cultivation method for dendrobium officinale and cultivation medium manure thereof |
| CN105248266A (en) * | 2015-11-04 | 2016-01-20 | 刘绥 | Planting method of dendrobium officinale on trees |
| CN105532405A (en) * | 2016-01-12 | 2016-05-04 | 云南天泉生物科技股份有限公司 | High-yield cultivation method for tree-grown dendrobium |
| CN105660334B (en) * | 2016-02-01 | 2019-02-12 | 广西顺昌隆生物科技有限公司 | A kind of method of dendrobium candidum potted landscape plantation |
| CN105660334A (en) * | 2016-02-01 | 2016-06-15 | 广西顺昌隆生物科技有限公司 | Method for planting potted dendrobium officinale |
| CN105766557A (en) * | 2016-03-15 | 2016-07-20 | 福建省邵武市二都国有林场 | Dendrobium candidum sprinkler irrigation device and cultivation method |
| CN105794612A (en) * | 2016-03-16 | 2016-07-27 | 中国科学院武汉植物园 | Construction method for tropical rainforest hanging garden |
| CN105900667A (en) * | 2016-05-23 | 2016-08-31 | 杨文明 | Method for planting dendrobium candidum in greenhouse |
| CN106550862A (en) * | 2016-11-15 | 2017-04-05 | 广西壮族自治区防城金花茶国家级自然保护区管理处 | The candidum tissue culturing seedling for exempting from seedling exercising ties up tree new method for cultivating |
| CN106613829A (en) * | 2016-11-16 | 2017-05-10 | 陈勇 | Method for planting dendrobium officinale on tree |
| CN108184588A (en) * | 2018-01-16 | 2018-06-22 | 安康市农业科学研究所 | A kind of efficient method for transplanting of konjaku tissue-cultured seedling |
| CN112056197A (en) * | 2020-09-07 | 2020-12-11 | 贵州沿河乌江生物科技发展有限公司 | Rapid tree culture method for dendrobium officinale tissue culture seedlings |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103548652B (en) | 2015-06-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103548652B (en) | Rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings | |
| CN103999672B (en) | A kind of method of non-polluted planting dendrobium candidum | |
| CN104025987A (en) | Dendrobium officinale planting method | |
| CN103766113B (en) | Method for planting anoectochilus formosanus in northern area | |
| CN103535238A (en) | Planting method of yellow peach trees | |
| CN104521715B (en) | Sealing water planting cutting propagation method for gardenias with flower buds | |
| CN103004409A (en) | Method for natural wild dendrobium officinale Kimura et Migo cultivation management | |
| CN107873286A (en) | A kind of mating system of passion fruit female parent seedling | |
| CN102612971A (en) | Rose cutting propagation method | |
| CN102960226B (en) | Dendrobium officinale Kimura et Migo tissue culture seedling acclimatization method | |
| CN105612988A (en) | Greenhouse planting method of dendrobium huoshanense | |
| CN105284314A (en) | Cultivation method for passion fruit virus-free seedling | |
| CN104885773A (en) | Method for rapidly cultivating early shaping tissue culture commodity seedlings of blueberries | |
| CN104303814A (en) | Bitter melon planting method | |
| CN103444530A (en) | Technology for field return-to-nature ex-situ plantation of wild anoectochilus roxburghii (Wall.) Lindl in Fujian | |
| CN106718735A (en) | A kind of method for planting dendrobium candidum in the tree | |
| CN103875515A (en) | Ex-vitro rooting method for tissue culture blueberry seedlings | |
| CN105918089A (en) | Soilless planting method for organic strawberries | |
| CN104770265A (en) | Organic pear tree planting process | |
| CN103460971A (en) | Method for improving transplanting survival rate of trichosanthes kirilowii tissue culture seedlings | |
| CN112154840A (en) | Cultivation method of elaeagnus conferta roxb pot culture | |
| CN103960030A (en) | Method for cultivating anoectochilus formosanus in wild imitation mode | |
| CN104770181A (en) | Strawberry planting method | |
| CN107197746A (en) | A kind of mating system of China fir field excellent resources | |
| CN113115652A (en) | Cutting propagation method of yolk fruit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150610 Termination date: 20171023 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |