CN107047217A - The tissue culture method for transplanting of xylophyta Chinese wax - Google Patents
The tissue culture method for transplanting of xylophyta Chinese wax Download PDFInfo
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- CN107047217A CN107047217A CN201710434661.9A CN201710434661A CN107047217A CN 107047217 A CN107047217 A CN 107047217A CN 201710434661 A CN201710434661 A CN 201710434661A CN 107047217 A CN107047217 A CN 107047217A
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- 239000012174 chinese wax Substances 0.000 title claims abstract description 34
- 238000012136 culture method Methods 0.000 title claims abstract description 19
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 claims abstract description 32
- 229920002554 vinyl polymer Polymers 0.000 claims abstract description 32
- 238000000034 method Methods 0.000 claims abstract description 30
- 238000002054 transplantation Methods 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 41
- 239000007921 spray Substances 0.000 claims description 17
- 239000003337 fertilizer Substances 0.000 claims description 15
- 239000011159 matrix material Substances 0.000 claims description 14
- 229910052902 vermiculite Inorganic materials 0.000 claims description 12
- 235000019354 vermiculite Nutrition 0.000 claims description 12
- 239000010455 vermiculite Substances 0.000 claims description 12
- 229920003023 plastic Polymers 0.000 claims description 9
- 239000004033 plastic Substances 0.000 claims description 9
- 239000000758 substrate Substances 0.000 claims description 9
- 238000005286 illumination Methods 0.000 claims description 8
- 238000011017 operating method Methods 0.000 claims description 7
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 6
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 6
- 239000006013 carbendazim Substances 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 5
- 239000002131 composite material Substances 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 230000000050 nutritive effect Effects 0.000 claims description 4
- 238000009423 ventilation Methods 0.000 claims description 4
- 239000003905 agrochemical Substances 0.000 claims description 2
- 230000004083 survival effect Effects 0.000 abstract description 24
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- 240000005323 Hoya carnosa Species 0.000 abstract description 4
- 238000013459 approach Methods 0.000 abstract description 3
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- 238000011534 incubation Methods 0.000 abstract description 2
- 210000001519 tissue Anatomy 0.000 description 27
- 241000196324 Embryophyta Species 0.000 description 20
- 210000004027 cell Anatomy 0.000 description 5
- 241000565359 Fraxinus chinensis Species 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
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- 235000001674 Agaricus brunnescens Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 238000004161 plant tissue culture Methods 0.000 description 2
- 210000003491 skin Anatomy 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 241000207834 Oleaceae Species 0.000 description 1
- 241001529246 Platymiscium Species 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 230000001754 anti-pyretic effect Effects 0.000 description 1
- 239000002221 antipyretic Substances 0.000 description 1
- 208000034158 bleeding Diseases 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000003500 flue dust Substances 0.000 description 1
- 208000006750 hematuria Diseases 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
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- 235000016709 nutrition Nutrition 0.000 description 1
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- 239000002699 waste material Substances 0.000 description 1
- 210000000051 wattle Anatomy 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of tissue culture method for transplanting of xylophyta Chinese wax, this method includes the transplanting of tissue-cultured seedling and the management process of transplanted seedling, by by after tissue culture transplantation of seedlings to seed plate, specific culture is carried out under conditions of seed plate is completely covered by vinyl cover, and carry out specific transplanted seedling management operation in follow-up incubation, make gradually adapt to transplant growing environment and natural environment after tissue culture transplantation of seedlings, promote to survive.Using the tissue culture method for transplanting of technical solution of the present invention, realize on the premise of without hardening treatment, high-survival rate of the tissue-cultured seedling through directly transplanting, it greatly simplify transplanting operating process, reducing transplanting process influences the factor of tissue culture shoot survival percent, make transplanting operation more stable, easy to control, the transplanting survival rate of Chinese wax tissue-cultured seedling can be significantly improved, and the healthy growth for Chinese wax plant after transplanting provides support, preservation and application also for high-quality tissue culture germ plasm resource provide effective technological approaches, with significant dissemination.
Description
Technical field
Moved the present invention relates to the tissue-cultured seedling in tissue-cultured seedling transplantation technique field in biotechnology, more particularly to xylophyta Chinese wax
Cultivating method.
Background technology
Chinese wax is the common name of Oleaceae Chinese wax platymiscium, and Chinese ash is named because putting wax insect in a suitable place to breed on tree.Chinese ash alias
Blue or green bulky wooden, white wattle is called, Northern Hemisphere temperate zone is distributed mainly on, is temperate zone deciduous tree, can be planted in China's most area,
Distribution from China northeast middle and south until the Yangtze river basin all, only a few extends to the torrid zone southwards.The wood of Chinese ash
Material is tough and tensile water-fast wet, preferably makes furniture, farm implements, glued board etc., and its branch can compile basket, is very important economical wood species.
Chinese wax or a kind of medicinal material plant, with hemostasis, myogenic, analgesic therapy, control medical in the effect such as bleeding, hematuria, lower blood, its bark
Make antipyretic, raw materials market demand is stable, with huge economic value potentiality.In addition, Chinese wax seeds body is rectified, trunk leads to
Straight graceful, with luxuriant foliage and spreading branches in leafy profusion and bud green, autumn leaves is orange, is excellent tree of fixing the sand, and the good shade tree of Shade effect；Available for lake
Bank greening, industrial and mining area greening, campus, the upper strata in gardens and large-scale greenery patches are afforested, or for the greening of slight surface soil alkalinity area；It is right
Anti- flue dust and improvement environment have certain effect.
Plant tissue culture technique is plant Sterile Culture Methods Used, is the theory for having totipotency according to plant cell, profit
With the in vitro organ of plant for example root, stem, leaf, stem apex, flower, fruit etc. organize (such as forming layer, epidermis, cortex, marrow cell,
Endosperm etc.) or cell (such as megaspore, microspore, body cell) and protoplast, in sterile and suitable artificial culture
Under the conditions of base and illumination, temperature etc. are artificial, callus, adventitious bud, adventitious root are induced, the skill of intact plant is eventually formed
Art.Tissue-cultured seedling is the theory for having totipotency according to plant cell, using outside shade, under sterile and suitable artificial condition,
The intact plant of cultivation.It is that can tissue-cultured seedling application and plant tissue culture technique success or failure that whether the transplanting of tissue-cultured seedling, which survives,
One of key technology, the sport technique segment is done well, can cause that all that has been achieved is spoiled, causes the waste of germ plasm resource and input.By planting
The tissue-cultured seedling that the breeding of thing tissue culture technique is obtained, due to being grown for a long time in artificial sterile, temperature and the Regulate Environment of illumination,
All there is very big difference with the seedling that is grown under natural conditions in terms of physiology, form, tissue-cultured seedling is transplanted to after matrix, by
Great changes are all there occurs in physiology, ecology, external environment etc., the inadaptability of tissue-cultured seedling is caused, causes survival rate relatively low.For
The transplanting survival rate of tissue-cultured seedling is improved, generally needs to carry out the operating procedures such as culture strong sprout, hardening to tissue-cultured seedling before transplanting, moves
The conditions such as control illumination, temperature, humidity are needed after cultivation, whole process influence factor is numerous, trivial operations, and whard to control, past
Toward more difficult raising survival rate, the production application of tissue-cultured seedling is limited.
The content of the invention
To solve the deficiency that prior art is present, the invention provides a kind of tissue culture transplantation of seedlings side of xylophyta Chinese wax
Method, the method for transplanting is directly transplanted to tissue-cultured seedling, is not required to by the hardening treatment to tissue-cultured seedling, can not only simplify transplanting stream
Journey, moreover it is possible to significantly improve transplanting survival rate.
To achieve the above object, the invention provides a kind of tissue culture method for transplanting of xylophyta Chinese wax, its feature exists
In：This method includes following operating procedure：
A, tissue-cultured seedling transplanting：The tissue-cultured seedling cleaned up is transplanted in the seed plate filled with matrix, after the completion of transplanting
Thin water is sprayed to more than 90%, seed plate then is completely covered using vinyl cover, is placed in 22 ± 2 DEG C of cultivation temperature, intensity of illumination
Transplanted seedling growth is cultivated in the environment of 2000~2500LX, light application time 12h；
B, transplanted seedling management：Tissue culture transplantation of seedlings opens vinyl cover in 6~7 days, shakes off the water droplet on vinyl cover and avoids
Enter in seed plate, seed plate is divulged information 30~50 minutes, vinyl cover is then covered into seed plate again, stayed during covering at the short side of seed plate
2~3cm gaps, after transplanted seedling grows 12~15 days, when transplanted seedling grows new stem apex and new root, all open vinyl cover, right
Transplanted seedling carries out routine growth management.
The tissue culture method for transplanting of the present invention, on the basis of the tissue-cultured seedling plant characteristic according to xylophyta Chinese wax, is used
To tissue-cultured seedling without hardening treatment, the method directly transplanted, by limiting after tissue culture transplantation of seedlings complete by vinyl cover
Specific culture is carried out under conditions of covering seed plate, and specific transplanted seedling management operation is carried out in follow-up incubation, makes group
It can gradually adapt to transplant environment and natural environment after training transplantation of seedlings, promote to survive, and significantly improve survival rate.Tissue culture transplantation of seedlings
Growth is first cultivated under conditions of seed plate is completely covered in vinyl cover afterwards, while limiting the humidity of matrix, the temperature for transplanting culture, light
By force, illumination condition, to simulate close to the culture environment before tissue culture transplantation of seedlings, after being cultivated through 6~7 days, transplanted seedling is gradually adapted to
Matrix environment, now needs to open vinyl cover and shakes off water droplet, it is to avoid water droplet falls into seed plate and produces pollution to matrix, while making seed plate
Ventilation, after plastic covering lid again after timing ventilation, now makes seed plate leave gap, so that environment and outside air in seed plate
Circulated, make transplanted seedling gradually reform of nature environment, continued to cultivate to after 12~15 days in such circumstances, treat that transplanted seedling is grown
New point and new root, show that transplanted seedling comes into the autotrophy stage, all open vinyl cover, carry out the Routine Management of transplanted seedling.Through
Aforesaid operations are crossed, Chinese wax tissue-cultured seedling is adapted to the method directly transplanted, transplanting operating process are not only greatly simplify, and reduce
During influence factor, make transplanting stable operation, easy to control, and the transplant survival of Chinese wax tissue-cultured seedling can be significantly improved
Rate, makes survival rate to more than 90%, and the healthy growth for Chinese wax plant after transplanting provides support.
As the restriction to above-mentioned technical proposal, following operating procedure is also included after the step b：
C, transplanted seedling strong seedling culture：Growth management is carried out to the transplanted seedling for growing new stem apex and new root, sprayed within every 3~4 days
Water is once, uniform after 15 days to spread fertilizer over the fields composite fertilizer and agricultural chemicals, applies fertilizer 1 time within every 7~10 days, and every 7~10 days sprinkling carbendazim once, are treated
After transplanted seedling grows 30~40 days, transplanted seedling plant is dark green, stalwartness, up to 15~20cm, you can be transplanted to nutritive cube.
Strong seedling culture is further carried out after transplanted seedling is survived, by limiting water and fertilizer management, Drug use administration, gives birth to transplanted seedling
Long more dark green, stalwartness, support is provided for follow-up quick healthy growth.
As the restriction to above-mentioned technical proposal, the cleaning requirement before tissue culture transplantation of seedlings described in step a is：
The healthy and strong tissue-cultured seedling with more than 3 roots is chosen, is taken out immediately from culture vessel, should not injure or touch off root,
And foundation portion culture medium, pure and fresh 2~3 times are gently washed away with clear water.
As the restriction to above-mentioned technical proposal, matrix described in step a selects vermiculite, and each seed plate dress vermiculite amount reaches
It is high to 4~5cm, and spray water to the water content of substrate is more than 70%.
As the restriction to above-mentioned technical proposal, seed plate described in step a selects long 53cm × wide 28cm × high 5cm standard
Plastics seedling dish.
As the restriction to above-mentioned technical proposal, 50 plants of tissue-cultured seedling are transplanted in seed plate described in step b.
As the restriction to above-mentioned technical proposal, after seed plate ventilation described in step b, again before plastic covering lid, to seed plate
Keep the skin wet.
As the restriction to above-mentioned technical proposal, seed plate described in step b carries out cultivation stage, root in plastic covering lid again
According to transplanted seedling form, kept the skin wet to seed plate within every 2~3 days.
As the restriction to above-mentioned technical proposal, composite fertilizer described in step c selects granular compound fertilizer, its dose be 5~
10g/ disks.
Further limit the cleaning standard of tissue-cultured seedling, the selection of matrix and substrates quantity, water content requirement, the selection of seed plate
And the management of water supplement, composite fertilizer apply management etc. and operated after tissue culture transplantation of seedlings quantity, transplanting in seed plate, more effectively to improve group
Transplantation of seedlings survival rate is trained, is that the healthy growth of transplanted seedling plays technical support.
In summary, using technical scheme, the tissue culture method for transplanting of the xylophyta Chinese wax of acquisition passes through
After tissue culture transplantation of seedlings to seed plate, specific culture is carried out under conditions of seed plate is completely covered by vinyl cover, and in follow-up training
The process of supporting carries out specific transplanted seedling management operation, makes gradually adapt to transplant environment and natural environment after tissue culture transplantation of seedlings,
Promote to survive, realize on the premise of without hardening treatment, high-survival rate of the tissue-cultured seedling through directly transplanting.The group of the present invention
Method for transplanting is trained, transplanting operating process has been greatly simplified with specification, the factor that transplanting process influence tissue-cultured seedling is survived is reduced,
Make transplanting operation more stable, easy to control, the transplanting survival rate of Chinese wax tissue-cultured seedling can be significantly improved, more than 90% is reached,
And the healthy growth for Chinese wax plant after transplanting provides technical support.In addition, also for high-quality tissue culture germ plasm resource preservation and
Using there is provided effective technological approaches, with significant dissemination.
Brief description of the drawings
The present invention is further detailed explanation with reference to the accompanying drawings and detailed description.
The transplanted seedling i.e. seedling growth conditions photo that Fig. 1 is obtained when being tissue culture transplantation of seedlings 6~7 days in the embodiment of the present invention；
Fig. 2 is the transplanted seedling for the going to culturing pot i.e. seedlings growth shape that the transplanting culture that the embodiment of the present invention is obtained is survived
State photo.
Embodiment
Below in conjunction with embodiment, technical scheme is clearly and completely described, it is clear that described
Embodiment is only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, this area
The every other embodiment that those of ordinary skill is obtained under the premise of creative work is not made, belongs to protection of the present invention
Scope.
Embodiment
The present embodiment is related to the transplanting of xylophyta Chinese wax tissue-cultured seedling.
Embodiment 1.1
The method for transplanting of Chinese wax tissue-cultured seedling is as follows：
A, tissue-cultured seedling transplanting：
A1, transplanting container selection：Using the standard plastic seed plate of non-volatility smell, the long 53cm of seed plate × wide 28cm × height
5cm, the light practicality of the seed plate；
A2, matrix selection：Gas permeability, moisture retention and certain nutrition will be possessed by being suitable for planting the matrix of tissue-cultured seedling, be selected
Select organic substrate --- vermiculite (new vermiculite), vermiculite standard is 1~3 millimeter, and each seed plate dress vermiculite amount is that 4~4.5cm is high, base
Matter is unfavorable for tissue-cultured seedling growth and moisturizing very little, and then spray water is to water content of substrate more than 70%, in case tissue culture transplantation of seedlings；
Cleaning before a3, tissue culture transplantation of seedlings：The healthy and strong tissue-cultured seedling with more than 3 roots (being needed on root with root hair) is chosen,
Remove the sealed membrane on blake bottle, gently taken out from blake bottle with tweezers immediately, it is to avoid here tissue-cultured seedling influences survival rate if withering, adopt
The culture medium in tissue-cultured seedling foundation portion is gently washed away with clear water, immature root and root hair, pure and fresh 2~3 times should not be hurt；
A4, transplanting operation：In the seed plate that the tissue-cultured seedling cleaned up is transplanted to water content of substrate more than 70%, during transplanting
Punched using seedling scuppit is planted, one plant of tissue-cultured seedling is put in each hole, and tissue-cultured seedling is gently put into and is compacted, and the seedling root of transplanting must
Stretch, in favor of slow seedling and growth, often disk standard is 50 plants, and then spraying thin water from Fine spray shower nozzle kettle (can in vaporific thin water
Avoid because water droplet is too big or the too many situation for causing seedling to be hurt of watering) to more than 90%, in the short period after the completion of watering
Seed plate is completely covered with colourless vinyl cover, it is to avoid and extraneous contact.Be placed in 22 ± 2 DEG C of cultivation temperature, intensity of illumination 2000~
Transplanted seedling growth is cultivated in the environment of 2500LX, light application time 12h；
B, transplanted seedling management：Tissue culture transplantation of seedlings opens vinyl cover in 6~7 days, shakes off a large amount of water droplets on vinyl cover and keeps away
Omit in seed plate, seed plate is divulged information 30 minutes, this stage transplanted seedling growth conditions is as shown in figure 1, spray a small amount of water, and often disk sprays
Water is 1500-2000 milliliters, and vinyl cover is then covered into seed plate again, stays more than 2cm to stitch at the short side of seed plate during covering
Gap, so as to air circulation, makes transplanted seedling gradually reform of nature environment, must conscientiously observe transplanted seedling form in this stage, 2~
Once, transplanted seedling grows new point and milky new root to 3 days water sprays after 12~15 days, and transplanted seedling comes into from form
During the autotrophy stage, vinyl cover is all opened, routine growth management is carried out to transplanted seedling；
C, transplanted seedling strong seedling culture：The transplanted seedling for growing new stem apex and new root carries out growth management, every 3 days water sprays one
It is secondary, 2000~2500 milliliters of injection flow rate；In order to promote transplanted seedling healthy growth, uniformly granular compound fertilizer, every 10 are spread fertilizer over the fields after 15 days
Its fertilising 1 time, first fertilising is 5 grams/disk；Second is 7 grams, is for the third time 10 grams；Meanwhile, for avoid mushroom breeding spread and
Growth, once, spray concentration is 800-1000 times of carbendazim to every 7~10 days sprinkling carbendazim；Treat transplanted seedling growth 30~40
After it, transplanted seedling plant height is up to 15~20cm, transplanted seedling well developed root system, and stalk is sturdy, leaf dark green, robust plant, and it grows shape
State is as shown in Figure 2, you can be transplanted to nutritive cube.
Aforesaid operations, once transplant 12 disks, transplanting survival rate is up to more than 92%, and the transplanting of conventional tissue-cultured seedling need to be through hardening
Stage, its transplanting survival rate only 75% or so；Compared to than method for transplanting of the invention is simple, and survival rate is significantly improved.
Embodiment 1.2
The method for transplanting of Chinese wax tissue-cultured seedling is as follows：
A, tissue-cultured seedling transplanting：
A1, transplanting container selection：Using the standard plastic seed plate of non-volatility smell, the long 53cm of seed plate × wide 28cm × height
5cm, the light practicality of the seed plate；
A2, matrix selection：Gas permeability, moisture retention and certain fertility will be possessed by being suitable for planting the matrix of tissue-cultured seedling, be selected
Select organic substrate --- vermiculite (new vermiculite), vermiculite standard is 1~3 millimeter, and each seed plate dress vermiculite amount is that 4.5~5cm is high, base
Matter is unfavorable for tissue-cultured seedling growth and moisturizing very little, water content of substrate more than 70% is then sprayed to, in case tissue culture transplantation of seedlings；
Cleaning before a3, tissue culture transplantation of seedlings：The healthy and strong tissue-cultured seedling with more than 3 roots (being needed on root with root hair) is chosen,
Remove the sealed membrane on blake bottle, gently taken out from blake bottle with tweezers immediately, it is to avoid here tissue-cultured seedling influences survival rate if withering, adopt
The culture medium in tissue-cultured seedling foundation portion is gently washed away with clear water, immature root and root hair should not be hurt, it is pure and fresh 2-3 times；
A4, transplanting operation：In the seed plate that the tissue-cultured seedling cleaned up is transplanted to water content of substrate more than 70%, during transplanting
Punched using seedling scuppit is planted, one plant of tissue-cultured seedling is put in each hole, and tissue-cultured seedling is gently put into and is compacted, and the seedling root of transplanting must
Stretch, root system group is unfavorable for slow seedling and growth together, often disk standard is 50 plants, then sprays thin water from Fine spray shower nozzle kettle
(Bao Shui refers to no water droplet, seems vaporific the same, can avoid because water droplet is too big or water causes seedling to be hurt too much) to 90% with
On, seed plate is completely covered with colourless vinyl cover in the short period after the completion of watering, it is to avoid and extraneous contact.It is placed in cultivation temperature 22
± 2 DEG C, 2000~2500LX of intensity of illumination, continued growth in the environment of light application time 12h；
B, transplanted seedling management：Tissue culture transplantation of seedlings opens vinyl cover in 6~7 days, shakes off a large amount of water droplets on vinyl cover and keeps away
Omit in seed plate, seed plate is divulged information 50 minutes, according to matrix content, spray 2000 milliliters of water, then cover vinyl cover again
Lid seed plate, stays 3cm gaps at the short side of seed plate during covering, so as to air circulation, makes tissue culture transplanted seedling gradually reform of nature ring
Border, transplanted seedling form must be conscientiously observed in this stage, once, transplanted seedling growth is new sharp and newborn after 12~15 days for moisturizing in 3 days
White new root, all opens vinyl cover, carries out transplanted seedling routine growth management；
C, transplanted seedling strong seedling culture：The transplanted seedling for growing new stem apex and new root carries out growth management, every 3 days water sprays one
It is secondary, 2000~2500 milliliters of injection flow rate；In order to promote transplanted seedling healthy growth, uniformly granular compound fertilizer, every 10 are spread fertilizer over the fields after 15 days
Its fertilising 1 time, first fertilising is 5 grams/disk；Second is 7 grams, is for the third time 10 grams；Meanwhile, in order to avoid mushroom breeding spreads
Growth, once, spray concentration is 800-1000 times of carbendazim to every 7~10 days sprinkling carbendazim；Treat transplanted seedling growth 30~40
After it, transplanted seedling plant height is up to 15~20cm, and transplanted seedling well developed root system, stalk is sturdy, leaf dark green, robust plant, you can transplant
To nutritive cube.
Aforesaid operations, once transplant 10 disks, and transplanting survival rate is up to 95.5%；The transplanting of conventional tissue-cultured seedling, need to be through hardening rank
Section, its transplanting survival rate only 75%；Compared to than method for transplanting of the invention is simple, and survival rate significantly improves more than 20%.
When vinyl cover being covered into seed plate again in step b, the gap left need to strictly control size, if gap is less than
2cm, is unfavorable for air and extraneous circulation, and air humidity is big, causes the seedling of not long new root easily to occur rotting and gradually dead
Phenomenon, if gap be more than 3cm, because transplanting time is short, tissue culture transplanted seedling does not grow new growing point and new root, be introduced into from
In the stage of supporting, influenceed suddenly by the external environment dried, easily occur transplanting seedling stem point and wilt, transplanted seedling is grown or is survived
Rate is severely impacted.
Comparative example
The present embodiment is related to vinyl cover covering seed plate in the tissue culture method for transplanting of the present invention and operated to transplanting survival rate
Influence.Take following method for transplanting：
A, tissue-cultured seedling transplanting：Take and embodiment step a identical operating procedures；
B, transplanted seedling management：Tissue culture transplantation of seedlings opens vinyl cover in 6~7 days, shakes off a large amount of water droplets on vinyl cover and keeps away
Omit in seed plate, seed plate is divulged information 30 minutes, it is then that vinyl cover is again complete according to 2000 milliliters of matrix content spray water
Seed plate is covered, vinyl cover is directly opened after 10 days, makes transplanted seedling gradually reform of nature environment, in 3 days this stages micro- spray repair water one
Secondary, transplanted seedling grows new point and white new root a bit after 12~15 days, and transplanted seedling is survived from morphological feature, there is part shifting
Plant seedling dead；
C, transplanted seedling strong seedling culture：With with embodiment step c identical operating procedures；Treat transplanted seedling growth 30~40
After it, transplanted seedling is up to 10~15cm, and transplanted seedling plant is high and low, and seedling is thin and delicate, and 5 disks are transplanted altogether, has a disk survival rate to only have
25%, statistics transplanting survival rate is 25~75%, and very undesirable, causing tens procedures, all that has been achieved is spoiled.
Comparative example is visible with comparative example, method for transplanting of the invention can significantly improve the transplanting of Chinese wax tissue-cultured seedling into
Motility rate.
In summary, tissue culture method for transplanting of the invention, is realized on the premise of without hardening treatment, tissue-cultured seedling
Through directly transplanting and strict control measure, transplanting survival rate is improved；Transplanting operating process has been greatly simplified with specification, has been reduced
The factor that transplanting process influence tissue-cultured seedling is survived, makes transplanting operation more stable, easy to control, can significantly improve Chinese wax tissue-cultured seedling
Transplanting survival rate, reaches more than 90%, and the healthy growth for Chinese wax plant after transplanting provides support, is also high-quality tissue culture
The preservation and application of germ plasm resource provide effective technological approaches, with significant dissemination.
Claims (9)
1. a kind of tissue culture method for transplanting of xylophyta Chinese wax, it is characterised in that：This method includes following operating procedure：
A, tissue-cultured seedling transplanting：The tissue-cultured seedling cleaned up is transplanted in the seed plate filled with matrix, sprayed after the completion of transplanting
Bao Shui to more than 90%, is then completely covered seed plate using vinyl cover, be placed in 22 ± 2 DEG C of cultivation temperature, intensity of illumination 2000~
Transplanted seedling growth is cultivated in the environment of 2500LX, light application time 12h；
B, transplanted seedling management：Tissue culture transplantation of seedlings opens vinyl cover in 6~7 days, shakes off the water droplet on vinyl cover and avoids falling into seedling
In disk, seed plate is divulged information 30~50 minutes, vinyl cover is then covered into seed plate again, stay at the short side of seed plate 2 during covering~
3cm gaps, after transplanted seedling grows 12~15 days, when transplanted seedling grows new stem apex and new root, all open vinyl cover, to transplanting
Seedling carries out routine growth management.
2. the tissue culture method for transplanting of xylophyta Chinese wax according to claim 1, it is characterised in that：In the step b
Also include following operating procedure afterwards：
C, transplanted seedling strong seedling culture：Growth management, every 3~4 days water sprays one are carried out to the transplanted seedling for growing new stem apex and new root
It is secondary, it is uniform after 15 days to spread fertilizer over the fields composite fertilizer and agricultural chemicals, apply fertilizer 1 time within every 7~10 days, every 7~10 days sprinkling carbendazim once, are waited to transplant
After seedling grows 30~40 days, transplanted seedling plant is dark green, stalwartness, up to 15~20cm, you can be transplanted to nutritive cube.
3. the tissue culture method for transplanting of xylophyta Chinese wax according to claim 1, it is characterised in that group described in step a
Training the cleaning requirement before transplantation of seedlings is：
The healthy and strong tissue-cultured seedling with more than 3 roots is chosen, is taken out immediately from culture vessel, should not be injured or touch off root, be used in combination
Clear water gently washes away foundation portion culture medium, pure and fresh 2~3 times.
4. the tissue culture method for transplanting of xylophyta Chinese wax according to claim 1, it is characterised in that：Base described in step a
Matter selects vermiculite, and each seed plate dress vermiculite amount reaches that 4~5cm is high, and spray water to the water content of substrate for 70% with
On.
5. the tissue culture method for transplanting of xylophyta Chinese wax according to claim 1, it is characterised in that：Seedling described in step a
Disk selects long 53cm × wide 28cm × high 5cm standard plastic seed plate.
6. the tissue culture method for transplanting of xylophyta Chinese wax according to claim 5, it is characterised in that：Seedling described in step b
50 plants of tissue-cultured seedling are transplanted in disk.
7. the tissue culture method for transplanting of xylophyta Chinese wax according to claim 1, it is characterised in that：Seedling described in step b
After disk ventilation, again before plastic covering lid, kept the skin wet to seed plate.
8. the tissue culture method for transplanting of xylophyta Chinese wax according to claim 1, it is characterised in that：Seedling described in step b
Disk carries out cultivation stage in plastic covering lid again, according to transplanted seedling form, is kept the skin wet to seed plate within every 2~3 days.
9. the tissue culture method for transplanting of xylophyta Chinese wax according to claim 2, it is characterised in that：It is multiple described in step c
Granular compound fertilizer is selected in Hefei, and its dose is 5~10g/ disks.
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| CN111758565A (en) * | 2019-08-07 | 2020-10-13 | 东北林业大学 | One-step rooting and transplanting technology for fraxinus mandshurica tissue culture seedlings |
| CN115804315A (en) * | 2022-12-28 | 2023-03-17 | 中国科学院遗传与发育生物学研究所农业资源研究中心 | Method for managing secondary forest close to natural forest by utilizing natural forest gap |
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| CN1288656A (en) * | 2000-11-09 | 2001-03-28 | 中国科学院石家庄农业现代化研究所 | Fast seedling growing mehtod for Chinese flowering crabapple zhumei |
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| CN111758565B (en) * | 2019-08-07 | 2022-04-01 | 东北林业大学 | One-step rooting and transplanting technology for fraxinus mandshurica tissue culture seedlings |
| CN115804315A (en) * | 2022-12-28 | 2023-03-17 | 中国科学院遗传与发育生物学研究所农业资源研究中心 | Method for managing secondary forest close to natural forest by utilizing natural forest gap |
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