CN106359090A - Method for culturing and planting organic Dendrobium officinale - Google Patents
Method for culturing and planting organic Dendrobium officinale Download PDFInfo
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- CN106359090A CN106359090A CN201610759959.2A CN201610759959A CN106359090A CN 106359090 A CN106359090 A CN 106359090A CN 201610759959 A CN201610759959 A CN 201610759959A CN 106359090 A CN106359090 A CN 106359090A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The invention relates to a method for culturing and planting organic Dendrobium officinale. The method comprises the following steps: A, selecting plants having pure varietal characteristics and growing robustly, reserving seeds for planting, and ensuring that no other dendrobe variety exists within a one-kilometer radius of the field for the cultivation of the seeds; B, collecting capsular fruits, performing afterripening, disinfecting with alcohol and sodium hypochlorite, and performing sterile seeding; C, inoculating protocorms to a culture medium composed of an MS (Murashige-Skoog) basal culture medium, 0.01-0.5mg/L 6-benzyladenine, 0.01-0.5mg/L alpha-naphthylacetic acid, 20% potato juice and 5g/L agar, and performing protocorm proliferation and differentiation culture; D, before transplanting, sufficiently cleaning the tissue culture seeds in tissue culture bottles; and E, transplanting the cleaned tissue culture seeds to a greenhouse for planting. The method is beneficial to healthy growth of dendrobe and can avoid disease and insect damage, thereby ensuring the quality of Dendrobium officinale; nutrition ingredients of the organic Dendrobium officinale are approximate to those of wild Dendrobium officinale; and the organic Dendrobium officinale has a high medicinal value.
Description
Technical field
The invention belongs to plant cultivation technology improvement areas, the cultivation plantation side of more particularly, to a kind of organic Herba Dendrobii
Method.
Background technology
Herba Dendrobii is orchid, and Herba Dendrobii can be divided into tens of kinds of Dendrobium denneanum Kerr., Dendrobium nobile Lindl, horsewhip etc., Herba Dendrobii be Herba Dendrobii it
Superfine product, it because epidermis be in ferrum green and gain the name, it is grown in the in the shade part of overhanging cliff, and root does not bury, have nourishing Tianjin,
Health invigorating, strengthen the spleen and stomach, hepatoprotective function of gallbladder promoting, strong muscle lipid-loweringing, reduce blood glucose, suppression tumor, bright eyes, nourish skin and prolong
The effects such as year lengthens one's life." Chinese pharmacy dictionary " claims Herba Dendrobii " specially control the gas-liquid of lung stomach, gas-liquid rushes prosperous, kidney water spontaneous ".Kind
In Yin-nourishing and body fluid promoting, treat all diseases of Yin and fluid deficiency.Shennong's Herbal is recorded: " the Herba Dendrobii eliminating impediment therapeutic method to keep the adverse QI flowing downwards, tonifying five ZANG-organses asthenia sea are thin, by force
Cloudy benefit essence, long term usage, thick the intestines and stomach ", claim it to have the effect of " main damaging the spleen and stomach, the eliminating impediment therapeutic method to keep the adverse QI flowing downwards, tonifying five ZANG-organses asthenia ".
Herba Dendrobii, also known as Herba hedyotis costatae, is the perennial herbaceous plant Herba Dendrobii of growing nonparasitically upon another plant of orchid family Dendrobium
The stem of dendrobiumofficinale kimura et migo, gains the name because epidermis is in ferrum green, is a kind of precious open country
Raw medicinal plants.It is born in height above sea level and reach on the dark and damp rock in 1600 meters of mountain region half, the ring of happiness warm and moist weather and half cloudy half light
Border, can not resist cold.Generally individually it is resistant to -5 DEG C of low temperature.Herba Dendrobii can be divided into tens of kinds of Dendrobium denneanum Kerr., Dendrobium nobile Lindl, horsewhip etc., and Herba Dendrobii is stone
The superfine product of dry measure used in former times.Herba Dendrobii has the medical value of uniqueness, is used as medicine with its stem, and " Chinese Pharmacopoeia " is recorded Herba Dendrobii and had stomach reinforcing life
The effect of Tianjin, nourishing YIN and clearing away heat.In the Tang Dynasty medical science classics " Taoist Scriptures ", Herba Dendrobii is listed in " first of Chinese nine big Herba mesonae chinensis ", from
Since Tang and Song Dynasty, it has been listed in royal tribute always.
Herba Dendrobii is listed in " first of Chinese nine big Herba mesonae chinensis ", since the Tang and Song Dynasty, has been listed in royal tribute always.Ferrum is current
The main consumption market of skin Herba Dendrobii also mainly carries in the Zhejiang of China and Shanghai one, and also there is little pin expense in the big city such as Beijing；
In addition it is exactly Hongkong and Macro and south east asia, western developed country also has import from China in the past few decades for a long time, in the last few years, with
The propagation of TCM Culture, consumption figure is also expanding.
Herba Dendrobii, is one of the most precious kind in China's orchid family Dendrobium Sw.Exempt from because it has enhancing body
The effects such as epidemic disease power, antitumor, defying age and effective mitigation hepatic injury, quilt in the version Pharmacopoeia of the People's Republic of China in 2010
Individually it is classified as medical material simply, due to the destruction of artificially excessively excavation and ecological environment, its own low reproduction rate in addition, wild iron sheet
Dendrobium Plants are day by day exhausted, are classified as the rare or endangered species of two grades of focused protections by country.Now, artificial culture plantation iron sheet
Herba Dendrobii has become the main path meeting great market demand.Herba Dendrobii system orchid family Dendrobium Sw, seed is minimum, no embryo
Breast, needs under natural conditions could sprout with some mycosymbiosises, and germination percentage only ten thousand in the case of nature/, and cultivar
There is degradation phenomena.
In recent years, some scientific research institutions of China and business unit are carried out to the tissue culture of Herba Dendrobii and artificial cultivation technique
Substantial amounts of explore and test, achieve some achievements, also so that in recent years artificial culture Herba Dendrobii yield increased.But
It is that the artificial culture of Herba Dendrobii yet suffers from that seeding propagation speed is slow, training tissue culture seedling and transplanting survival rate is low, artificial growth
The low problem of product active constituent content, seriously constrains industrialization, the large-scale development of Herba Dendrobii.Additionally, Herba Dendrobii
General field planting with growth of weeds, and pest and disease damage can be vulnerable to.
But, prior art in large-scale production, from seed to the seedlings of Dendrobium officinale of finished product Seedling of taking root cultivation when
Between for 150 days when, qualified Seedling only accounts for the 50% about of total Seedling amount；During by 180 days, qualified Seedling accounts for the 80% about of total Seedling amount；
And need the longer seedling exercising time just can have more than 90% transplanting survival rate.
Content of the invention
It is an object of the invention to provide a kind of cultivation implantation methods of organic Herba Dendrobii are it is intended to solve above-mentioned technology
Problem.
The present invention is achieved in that a kind of cultivation implantation methods of organic Herba Dendrobii, and methods described includes following step
Rapid:
A, selection breediness are pure, robust growth plant is reserved seed for planting, and seed-plot has an area of no other species Herba Dendrobiis in 1 km；?
June full-bloom stage, is pollinated, and female parent extracts lip after pollination immediately, timely hanging scutcheon；Pollination then October with
Afterwards, when capsule starts to turn yellow, full fruit is selected to harvest together with stem；
B, the capsule of harvesting, through after-ripening, carry out aseptic seeding using after ethanol+hypochlorite disinfectant；Connect from tissue culture bottle
Kind, culture medium is 1/2ms minimal medium+murphy juice 15%+agar 5g/l；Postvaccinal tissue culture bottle is put into led
In light incubator, led light is cultivated the temperature inside the box and is controlled at 23 DEG C, is divided into illumination 12 hours and dark 12 hours daily,
Light quality is red indigo plant mixed light, HONGGUANG: blue light is 1: 1, and intensity of illumination controls in 1500-2500lux；After 18 days, more than 98%
Embryo expand sprout formed green protocorm；
C, protocorm is forwarded to ms minimal medium+6- benzyladenine 0.01～0.5mg/l+ α-naphthalene acetic acid 0.01
Carry out Protocorm Multiplication, differentiation culture in the culture medium of～0.5mg/l+ murphy juice 20%+agar 5g/l；Group after switching
Training bottle is put in led light incubator, and led light is cultivated the temperature inside the box and controlled at 23 DEG C, with illumination 12 hours and dark 6
Hour is a cycle, three days four cycles, and light quality is red indigo plant mixed light, HONGGUANG: blue light is 4: 3, and intensity of illumination controls
2000-2500lux ；After 33 days, more than 98% protocorm has phyllogen stem and seedling to occur, seedling height 1～2cm；
D, before being transplanted, need the tissue cultured seedling in tissue culture bottle is sufficiently cleaned: with tweezers by 3 to 5 young plants by
Step pulls out from bottle, and action is light and slow to be prevented from hindering Seedling；Take 3 to 5 young plants to rinse agar in clear water, gently rubbed with finger and wash root
Portion is it is ensured that no agar remains；By bottle cleaning, prevent in the long-time soaking water of excessive Seedling, often change clear water after 30 bottles of cleaning；Drift
Vertically it is placed in basket after the excessively secondary clear water of Seedling washing agar off and wait to transplant, the non-woven fabrics moisturizing of surface covering wet；
E, by the tissue culture transplantation of seedlings after cleaning to greenhouse gardening: booth addressing is logical in the mountain top of 300～600 meters of height above sea level depression
At wind, booth is furnished with sunshade net and sprinkling irrigation equipment, build in booth multiple wide about 1.2～1.5 meters, high 80cm bamboo or
The seedbed of steelframe, is furnished with the wide passage of 40～50cm between seedbed, seedbed bottom spreads the thick cultivation base of one layer of 10～12cm
Matter, Cortex Pini and pine sawdust that cultivation matrix is crossed for fermentation process, mixing sheep stool in cultivation matrix；Select anosis, healthy and strong, size
Uniform tissue cultured seedling is colonized, and by 3～5 plants every clump, spacing in the rows 10cm, the specification of line-spacing 13cm are planted in cultivation matrix
On, during field planting, root is completely covered with cultivation matrix and is advisable；Transplant in 7 days, in booth: intensity of illumination controls 1000～
10000lux, at 15～25 DEG C, relative air humidity controls 80%～90% to temperature control, using blade spraying and ground
Keeping the skin wet, the ph value of water is 5～6 for watering；Trophophase after transplanting 7 days, in booth: intensity of illumination controls
10000～15000lux, day temperature controls at 16～21 DEG C, and nocturnal temperature controls at 10～15 DEG C, and air is relatively
Humid control 70%～80%, sprinkles water to keep the skin wet using blade spraying and ground, and the ph value of water is 5～6.
The further technical scheme of the present invention is: in described step e, the fermentation treatments of cultivation matrix are: high in summer
Warm season, in booth, substrate is piled the substrate heap that 1.2m is high, 2.5m is wide, substrate is squirted, so that water content of substrate is exceeded
80%, then use covered rearing with plastic film substrate heap, be enclosed within booth, opened thin film turning once every 10 days, turning 5-6
Secondary, be changed into after brown after substrate, substrate is spread out pack after cooling standby；When cultivation matrix uses, add water to water content 60%,
And the 0.5% interpolation decay sheep excrement according to substrate gross mass, with artificial or blender mix homogeneously.
The invention has the beneficial effects as follows: be conducive to the healthy growth of Herba Dendrobii, and be avoided that pest and disease damage occurs, using acid water
Matter, without chemical fertilizer, the growing environment making Herba Dendrobii, closer to natural environment, thus ensureing the quality of Herba Dendrobii, and is sought
Form part close to dendrobium officinale, medical value is high.
Specific embodiment
The cultivation implantation methods of organic Herba Dendrobii that the present invention provides, details are as follows for it:
Step a, selection breediness is pure, robust growth plant is reserved seed for planting, and seed-plot has an area of no other species stones in 1 km
Dry measure used in former times；In June full-bloom stage, pollinated, female parent extracts lip after pollination immediately, timely hanging scutcheon；Pollination then 10
After month, when capsule starts to turn yellow, full fruit is selected to harvest together with stem.
Step b, the capsule of harvesting, through after-ripening, carries out aseptic seeding using after ethanol+hypochlorite disinfectant；From tissue culture
Bottle is inoculated, and culture medium is 1/2ms minimal medium+murphy juice 15%+agar 5g/l；By postvaccinal tissue culture bottle
Put in led light incubator, led light is cultivated the temperature inside the box and controlled at 23 DEG C, be divided into illumination 12 hours and dark daily
12 hours, light quality was red indigo plant mixed light, HONGGUANG: blue light is 1: 1, and intensity of illumination controls in 1500-2500lux；After 18 days,
More than 98% embryo expands sprouts the protocorm forming green.
Step c, protocorm is forwarded to ms minimal medium+6- benzyladenine 0.01～0.5mg/l+ α-naphthalene
Carry out Protocorm Multiplication, differentiation culture in the culture medium of acetic acid 0.01～0.5mg/l+ murphy juice 20%+agar 5g/l；
Tissue culture bottle after switching is put in led light incubator, and led light is cultivated the temperature inside the box and controlled at 23 DEG C, little with illumination 12
When and be within dark 6 hours a cycle, in three days four cycles, light quality is red indigo plant mixed light, HONGGUANG: blue light is 4: 3, and illumination is strong
Degree controls in 2000-2500lux；After 33 days, more than 98% protocorm has phyllogen stem and seedling to occur, and seedling height 1～
2cm .
Step d, before being transplanted, needs the tissue cultured seedling in tissue culture bottle is sufficiently cleaned: with tweezers by 3 to 5
Young plant progressively pulls out from bottle, and action is light and slow to be prevented from hindering Seedling；3 to 5 young plants are taken to rinse agar in clear water, with finger gently
Rub and wash root it is ensured that no agar remains；By bottle cleaning, prevent in the long-time soaking water of excessive Seedling, often change clear after 30 bottles of cleaning
Water；Vertically it is placed in basket after the excessively secondary clear water of Seedling rinsing agar and wait to transplant, the non-woven fabrics moisturizing of surface covering wet.
Step e, by the tissue culture transplantation of seedlings after cleaning to greenhouse gardening: booth addressing is on the mountain of 300～600 meters of height above sea level
Top depression ventilation, booth is furnished with sunshade net and sprinkling irrigation equipment, builds multiple wide about 1.2～1.5 meters, high 80cm in booth
Bamboo or steelframe seedbed, be furnished with the wide passage of 40～50cm between seedbed, it is thick that seedbed bottom spreads one layer of 10～12cm
Cultivation matrix, Cortex Pini and pine sawdust that cultivation matrix is crossed for fermentation process, mixing sheep stool in cultivation matrix；Selection is anosis,
Tissue cultured seedling healthy and strong, of uniform size is colonized, by 3～5 plants every clump, spacing in the rows 10cm, the specification of line-spacing 13cm plant in
On cultivation matrix, during field planting, root is completely covered with cultivation matrix and is advisable；Transplant in 7 days, in booth: intensity of illumination controls
1000～10000lux, at 15～25 DEG C, relative air humidity controls 80%～90% to temperature control, using blade spray
Sprinkle water and to keep the skin wet in mist and ground, the ph value of water is 5～6；Trophophase after transplanting 7 days, in booth: intensity of illumination
Control in 10000～15000lux, day temperature controls at 16～21 DEG C, and nocturnal temperature controls at 10～15 DEG C, empty
Gas phase to humid control 70%～80%, sprinkles water to keep the skin wet using blade spraying and ground, and the ph value of water is 5～6.
In described step e, the fermentation treatments of cultivation matrix are: in summer high temperature season, in booth, substrate is piled
The substrate heap that 1.2m is high, 2.5m is wide, substrate is squirted, makes water content of substrate more than 80%, then use covered rearing with plastic film base
Matter heap, is enclosed within booth, opens thin film turning once every 10 days, and turning 5-6 time is changed into after brown after substrate, by base
Matter packs standby after spreading cooling out；When cultivation matrix uses, add water to water content 60%, and 0.5% according to substrate gross mass
Add decay sheep excrement, with artificial or blender mix homogeneously.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Any modification, equivalent and improvement made within god and principle etc., should be included within the scope of the present invention.
Claims (2)
1. a kind of cultivation implantation methods of organic Herba Dendrobii are it is characterised in that the method comprising the steps of:
A, selection breediness are pure, robust growth plant is reserved seed for planting, and seed-plot has an area of no other species Herba Dendrobiis in 1 km；?
June full-bloom stage, is pollinated, and female parent extracts lip after pollination immediately, timely hanging scutcheon；Pollination then October with
Afterwards, when capsule starts to turn yellow, full fruit is selected to harvest together with stem；
B, the capsule of harvesting, through after-ripening, carry out aseptic seeding using after ethanol+hypochlorite disinfectant；Connect from tissue culture bottle
Kind, culture medium is 1/2ms minimal medium+murphy juice 15%+agar 5g/l；Postvaccinal tissue culture bottle is put into led
In light incubator, led light is cultivated the temperature inside the box and is controlled at 23 DEG C, is divided into illumination 12 hours and dark 12 hours daily,
Light quality is red indigo plant mixed light, HONGGUANG: blue light is 1: 1, and intensity of illumination controls in 1500-2500lux；After 18 days, more than 98%
Embryo expand sprout formed green protocorm；
C, protocorm is forwarded to ms minimal medium+6- benzyladenine 0.01～0.5mg/l+ α-naphthalene acetic acid 0.01
Carry out Protocorm Multiplication, differentiation culture in the culture medium of～0.5mg/l+ murphy juice 20%+agar 5g/l；Group after switching
Training bottle is put in led light incubator, and led light is cultivated the temperature inside the box and controlled at 23 DEG C, with illumination 12 hours and dark 6
Hour is a cycle, three days four cycles, and light quality is red indigo plant mixed light, HONGGUANG: blue light is 4: 3, and intensity of illumination controls
2000-2500lux ；After 33 days, more than 98% protocorm has phyllogen stem and seedling to occur, seedling height 1～2cm；
D, before being transplanted, need the tissue cultured seedling in tissue culture bottle is sufficiently cleaned；With tweezers by 3 to 5 young plants by
Step pulls out from bottle, and action is light and slow to be prevented from hindering Seedling；Take 3 to 5 young plants to rinse agar in clear water, gently rubbed with finger and wash root
Portion is it is ensured that no agar remains；By bottle cleaning, prevent in the long-time soaking water of excessive Seedling, often change clear water after 30 bottles of cleaning；Drift
Vertically it is placed in basket after the excessively secondary clear water of Seedling washing agar off and wait to transplant, the non-woven fabrics moisturizing of surface covering wet；
E, by the tissue culture transplantation of seedlings after cleaning to greenhouse gardening: booth addressing is logical in the mountain top of 300～600 meters of height above sea level depression
At wind, booth is furnished with sunshade net and sprinkling irrigation equipment, build in booth multiple wide about 1.2～1.5 meters, high 80cm bamboo or
The seedbed of steelframe, is furnished with the wide passage of 40～50cm between seedbed, seedbed bottom spreads the thick cultivation base of one layer of 10～12cm
Matter, Cortex Pini and pine sawdust that cultivation matrix is crossed for fermentation process, mixing sheep stool in cultivation matrix；Select anosis, healthy and strong, size
Uniform tissue cultured seedling is colonized, and by 3～5 plants every clump, spacing in the rows 10cm, the specification of line-spacing 13cm are planted in cultivation matrix
On, during field planting, root is completely covered with cultivation matrix and is advisable；Transplant in 7 days, in booth: intensity of illumination controls 1000～
10000lux, at 15～25 DEG C, relative air humidity controls 80%～90% to temperature control, using blade spraying and ground
Keeping the skin wet, the ph value of water is 5～6 for watering；Trophophase after transplanting 7 days, in booth: intensity of illumination controls
10000～15000lux, day temperature controls at 16～21 DEG C, and nocturnal temperature controls at 10～15 DEG C, and air is relatively
Humid control 70%～80%, sprinkles water to keep the skin wet using blade spraying and ground, and the ph value of water is 5～6.
2. according to claim 1 it is characterised in that in described step e the fermentation treatments of cultivation matrix be: in the summer
Season high temperature season, in booth, substrate is piled the substrate heap that 1.2m is high, 2.5m is wide, substrate is squirted, makes water content of substrate
More than 80%, then use covered rearing with plastic film substrate heap, be enclosed within booth, opened thin film turning once every 10 days, turning
5-6 time, be changed into after brown after substrate, substrate is spread out pack after cooling standby；When cultivation matrix uses, add water to water content
60%, and the 0.5% interpolation decay sheep excrement according to substrate gross mass, with artificial or blender mix homogeneously.
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| CN201610759959.2A CN106359090A (en) | 2016-08-30 | 2016-08-30 | Method for culturing and planting organic Dendrobium officinale |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108770668A (en) * | 2018-06-22 | 2018-11-09 | 遵义市播州区惠联生态农业发展有限公司 | A kind of purple dendrobium capsule seed seedling-raising technique |
| CN108967157A (en) * | 2018-07-12 | 2018-12-11 | 厦门谷草农业科技有限公司 | A kind of novel officinal dendrobium stem plantation method |
| CN109122256A (en) * | 2018-08-01 | 2019-01-04 | 信阳农林学院 | A kind of dendrobium candidum hanging bag implantation methods |
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| CN104855262A (en) * | 2015-06-05 | 2015-08-26 | 杭州正德农业开发有限公司 | Method for cultivating and planting organic dendrobium candidum |
| CN105145146A (en) * | 2015-09-18 | 2015-12-16 | 湖南农业大学 | Method for seedling and planting dendrobii officmalis caulis by adopting LED light source |
| CN105706921A (en) * | 2014-11-30 | 2016-06-29 | 天津榛发科技有限责任公司 | Dendrobium officinale tissue culture method based on enclosed-type plant factory |
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| CN102057867A (en) * | 2009-11-14 | 2011-05-18 | 斯金平 | Light control method for dendrobium officinale tissue culture |
| CN105706921A (en) * | 2014-11-30 | 2016-06-29 | 天津榛发科技有限责任公司 | Dendrobium officinale tissue culture method based on enclosed-type plant factory |
| CN104855262A (en) * | 2015-06-05 | 2015-08-26 | 杭州正德农业开发有限公司 | Method for cultivating and planting organic dendrobium candidum |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108770668A (en) * | 2018-06-22 | 2018-11-09 | 遵义市播州区惠联生态农业发展有限公司 | A kind of purple dendrobium capsule seed seedling-raising technique |
| CN108967157A (en) * | 2018-07-12 | 2018-12-11 | 厦门谷草农业科技有限公司 | A kind of novel officinal dendrobium stem plantation method |
| CN109122256A (en) * | 2018-08-01 | 2019-01-04 | 信阳农林学院 | A kind of dendrobium candidum hanging bag implantation methods |
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