CN107047217B - Tissue culture seedling transplanting method for woody plant fraxinus chinensis - Google Patents
Tissue culture seedling transplanting method for woody plant fraxinus chinensis Download PDFInfo
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- 238000002054 transplantation Methods 0.000 claims description 11
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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Abstract
The invention discloses a tissue culture seedling transplanting method for woody plant white wax, which comprises the steps of transplanting tissue culture seedlings and managing the transplanted seedlings, wherein after the tissue culture seedlings are transplanted to a seedling tray, specific culture is carried out under the condition that the seedling tray is completely covered by a plastic cover, and specific transplanted seedling management operation is carried out in the subsequent culture process, so that the transplanted tissue culture seedlings can gradually adapt to the transplanting growth environment and the natural environment, and the survival is promoted. By adopting the tissue culture seedling transplanting method provided by the technical scheme of the invention, the high survival rate of the tissue culture seedlings after direct transplanting is realized on the premise of not hardening the seedlings, the transplanting operation process is greatly simplified, the factors influencing the survival rate of the tissue culture seedlings in the transplanting process are reduced, the transplanting operation is more stable and easy to control, the transplanting survival rate of the Fraxinus chinensis tissue culture seedlings can be remarkably improved, the support is provided for the healthy growth of the transplanted Fraxinus chinensis plants, an effective technical approach is provided for the preservation and application of high-quality tissue culture germplasm resources, and the method has remarkable popularization significance.
Description
Technical Field
The invention relates to the technical field of tissue culture seedling transplantation in biotechnology, in particular to a tissue culture seedling transplantation method of woody plant fraxinus chinensis.
Background
Fraxinus chinensis is a generic name for plants in the genus Fraxinus of the family Oleaceae, and is named for the stocking of the wax insect on the tree. The white ash trees are also called areca-nut trees and white wattle trees, are mainly distributed in the temperate zone of the northern hemisphere, are trees with fallen leaves in the temperate zone, can be planted in most areas of China, are visible in the distribution range from the northeast, the middle and the south of China to the Yangtze river basin, and rarely extend to the south to the tropical zone. The ash tree is tough and water-wet resistant, and can be used for making furniture, farm implements and plywood, and its branch can be used for weaving basket, so that it is an important economic section tree species. The white wax is also a medicinal plant, has the effects of stopping bleeding, promoting granulation, relieving pain, treating bleeding, hematuria, bleeding and the like, and the bark of the white wax is used as a heat clearing medicament in traditional Chinese medicine, so that the raw material market demand is stable, and the white wax has great economic value potential. In addition, the fraxinus chinensis has a regular shape, a straight and elegant trunk, luxuriant and fresh green branches and leaves, and orange yellow autumn leaves, and is an excellent sand-fixing tree and a street tree with good shading effect; can be used for lake bank greening, industrial and mining area greening, campus greening, garden greening of the upper layer of large green land, or greening of mild soil alkaline zone; has certain functions of resisting smoke dust and improving environment.
A plant tissue culture technique, namely a plant sterile culture technique, is a technique for inducing callus, adventitious buds and adventitious roots by using tissues (such as cambium, epidermis, cortex, medulla part cells, endosperm and the like) or cells (such as megaspore, microspore, somatic cells and the like) and protoplast of isolated organs of a plant body according to the theory that plant cells have totipotency, and under the artificial conditions of sterile and proper artificial culture medium, illumination, temperature and the like, finally forming a complete plant. The tissue culture seedling is a complete plant which is cultured by utilizing an explant under sterile and proper artificial conditions according to the theory that plant cells have totipotency. Whether the tissue culture seedling survives or not is one of key technologies of whether the tissue culture seedling can be applied and popularized or not and whether the plant tissue culture technology fails or not, and the technical link is not good, so that the previous work is abandoned, and the germplasm resources and the investment are wasted. The tissue culture seedling obtained by the propagation of the plant tissue culture technology grows in artificial aseptic, temperature and illumination regulation and control environment for a long time, and has great difference from the seedling growing under natural conditions in the aspects of physiology, morphology and the like. In order to improve the transplanting survival rate of the tissue culture seedlings, the operation steps of culturing strong seedlings, hardening seedlings and the like are generally needed for the tissue culture seedlings before transplanting, and the conditions of illumination, temperature, humidity and the like need to be controlled after transplanting.
Disclosure of Invention
In order to solve the defects of the prior art, the invention provides the tissue culture seedling transplanting method of the woody plant fraxinus chinensis.
In order to achieve the purpose, the invention provides a tissue culture seedling transplanting method of woody plant fraxinus chinensis, which is characterized in that: the method comprises the following operation steps:
a. transplanting the tissue culture seedlings: transplanting the cleaned tissue culture seedlings into a seedling tray filled with a matrix, spraying thin water to over 90 percent after the transplanting is finished, then completely covering the seedling tray with a plastic cover, and culturing the transplanted seedlings to grow under the environment of a culture temperature of 22 +/-2 ℃, an illumination intensity of 2000-2500 LX and an illumination time of 12 h;
b. managing the transplanted seedlings: and (3) uncovering the plastic cover after the tissue culture seedlings are transplanted for 6-7 days, shaking off water drops on the plastic cover and avoiding falling into the seedling tray, ventilating the seedling tray for 30-50 minutes, then covering the seedling tray with the plastic cover again, reserving a gap of 2-3 cm at the short edge of the seedling tray during covering, completely uncovering the plastic cover when the transplanted seedlings grow new stem tips and new roots after the transplanted seedlings grow for 12-15 days, and performing conventional growth management on the transplanted seedlings.
According to the tissue culture seedling transplanting method, on the basis of the characteristics of the tissue culture seedlings of the woody plant white wax, the method that the tissue culture seedlings are directly transplanted without hardening seedlings is adopted, the specific culture is carried out under the condition that the seedling tray is completely covered by the plastic cover after the tissue culture seedlings are transplanted, and the specific transplanted seedling management operation is carried out in the subsequent culture process, so that the tissue culture seedlings can gradually adapt to the transplanting environment and the natural environment after being transplanted, the survival is promoted, and the survival rate is remarkably improved. After the tissue culture seedlings are transplanted, the tissue culture seedlings are cultured and grown under the condition that a seedling tray is completely covered by a plastic cover, meanwhile, the humidity of a matrix, the temperature, the light intensity and the illumination condition of transplanting culture are limited to simulate the culture environment close to that before the tissue culture seedlings are transplanted, after 6-7 days of culture, the transplanted seedlings gradually adapt to the matrix environment, at the moment, the plastic cover needs to be uncovered and water drops need to be shaken off to avoid the water drops from falling into the seedling tray to pollute the matrix, the seedling tray is ventilated, the plastic cover is covered again after the ventilation is carried out for certain time, at the moment, gaps are reserved on the seedling tray to enable the environment in the seedling tray to be communicated with the outside air, the transplanted seedlings gradually adapt to the natural environment, after the seedling tray is continuously cultured for 12-15 days under the environment, new tips and new roots grow after the transplanted seedlings enter an autotrophic stage, the plastic covers are completely uncovered, and the conventional management. Through the operation, the method for adapting the tissue culture seedlings of the fraxinus chinensis to direct transplantation not only greatly simplifies the transplantation operation process, but also reduces the influence factors in the process, ensures that the transplantation operation is stable and easy to control, can obviously improve the transplantation survival rate of the tissue culture seedlings of the fraxinus chinensis, ensures that the survival rate reaches more than 90 percent, and provides support for the healthy growth of the transplanted fraxinus chinensis plants.
As a limitation to the above technical solution, the method further comprises the following operation steps after the step b:
c. strong seedling culture of the transplanted seedlings: and (3) carrying out growth management on the transplanted seedlings with new stem tips and new roots, spraying water once every 3-4 days, uniformly spreading compound fertilizer and pesticide after 15 days, applying fertilizer for 1 time every 7-10 days, spraying carbendazim once every 7-10 days, and transplanting the seedlings into a nutrition pot after the transplanted seedlings grow for 30-40 days, wherein the transplanted seedlings are dark green and robust, and are as high as 15-20 cm.
And after the transplanted seedlings survive, strong seedling culture is further carried out, and the transplanted seedlings grow more dense, green and robust by limiting water and fertilizer management and pesticide management, so that support is provided for subsequent quick and healthy growth.
As a limitation to the above technical solution, the requirement for cleaning the tissue culture seedling before transplantation in step a is as follows:
selecting robust tissue culture seedlings with more than 3 roots, immediately taking out the robust tissue culture seedlings from a culture container without damaging or bumping the roots, and slightly washing off the culture medium at the root base part with clear water for 2-3 times.
And b, as a limitation on the technical scheme, the substrate in the step a is vermiculite, the vermiculite loading amount of each seedling tray reaches 4-5 cm, and water is sprayed until the water content of the substrate is more than 70%.
As the limitation of the technical scheme, the seedling tray in the step a is a standard plastic seedling tray with the length of 53cm, the width of 28cm and the height of 5 cm.
And b, transplanting 50 tissue culture seedlings in the seedling tray in the step b as a limitation of the technical scheme.
And (c) as a limitation to the technical scheme, after the seedling tray is ventilated and before the plastic cover is covered again, replenishing water to the seedling tray.
And b, as a limitation on the technical scheme, in the stage of culturing the seedling tray covered with the plastic cover again, according to the shape of the transplanted seedlings, supplementing water to the seedling tray every 2-3 days.
As a limitation to the technical scheme, the compound fertilizer in the step c is a granular compound fertilizer, and the fertilizing amount of the granular compound fertilizer is 5-10 g/disc.
The cleaning standard of the tissue culture seedlings is further limited, the selection of the matrix and the requirements of the matrix amount and the water content are further limited, the selection of the seedling tray and the operations of transplanting quantity of the tissue culture seedlings in the seedling tray, water supplement management after transplanting, application management of compound fertilizer and the like are performed, so that the transplanting survival rate of the tissue culture seedlings is more effectively improved, and the technical support is provided for the healthy growth of the transplanted seedlings.
In summary, according to the tissue culture seedling transplanting method for the woody plant fraxinus disclosed by the technical scheme of the invention, after the tissue culture seedlings are transplanted to the seedling tray, the specific culture is carried out under the condition that the seedling tray is completely covered by the plastic cover, and the specific transplanted seedling management operation is carried out in the subsequent culture process, so that the transplanted tissue culture seedlings can gradually adapt to the transplanting environment and the natural environment, the survival is promoted, and the high survival rate of the tissue culture seedlings after direct transplanting is realized on the premise of not carrying out hardening treatment on the seedlings. The method for transplanting the tissue culture seedlings greatly simplifies and standardizes the transplanting operation process, reduces the factors influencing the survival of the tissue culture seedlings in the transplanting process, ensures that the transplanting operation is more stable and easy to control, can obviously improve the transplanting survival rate of the fraxinus chinensis tissue culture seedlings to more than 90 percent, and provides technical support for the healthy growth of the transplanted fraxinus chinensis plants. In addition, an effective technical approach is provided for the preservation and the application of high-quality tissue culture germplasm resources, and the method has obvious popularization significance.
Drawings
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
FIG. 1 is a photograph showing the growth state of a transplanted tissue culture seedling, i.e., a young seedling, obtained after the tissue culture seedling is transplanted for 6 to 7 days in the example of the present invention;
FIG. 2 is a photograph showing the growth state of a seedling which is a transplanted seedling which survives the transplanting culture and can be transferred to a culture pot according to an embodiment of the present invention.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples
This example relates to the transplantation of woody plant Cera chinensis tissue culture seedlings.
Example 1.1
The transplanting method of the fraxinus chinensis tissue culture seedlings comprises the following steps:
a. transplanting the tissue culture seedlings:
a1, selecting a transplanting container: adopting a standard plastic seedling tray without volatile smell, wherein the length of the seedling tray is 53cm, the width of the seedling tray is 28cm, and the height of the seedling tray is 5cm, and the seedling tray is portable and practical;
a2, selecting a substrate: the matrix suitable for planting the tissue culture seedlings has air permeability, moisture retention and certain nutrition, an organic matrix-vermiculite (new vermiculite) is selected, the standard of the vermiculite is 1-3 mm, the vermiculite loading amount of each seedling tray is 4-4.5 cm, the matrix is too little to be beneficial to the growth and moisture retention of the tissue culture seedlings, and then water is sprayed until the water content of the matrix is more than 70% for transplanting the tissue culture seedlings;
a3, cleaning the tissue culture seedlings before transplanting: selecting robust tissue culture seedlings with more than 3 roots (roots need to have root hairs), removing a sealing film on a culture bottle, immediately and lightly taking out the robust tissue culture seedlings from the culture bottle by using tweezers to avoid the influence on survival rate caused by withering of the tissue culture seedlings, lightly washing off a culture medium at the root bases of the tissue culture seedlings by using clear water, not damaging young roots and root hairs, and refreshing for 2-3 times;
a4, transplanting operation: transplanting clean tissue culture seedlings into a seedling tray with the substrate water content of more than 70%, digging holes by using a seedling planting shovel during transplanting, placing one tissue culture seedling in each hole, gently placing and compacting the tissue culture seedlings, stretching roots of the transplanted seedlings to be beneficial to seedling reviving and growth, wherein the standard of each tray is 50 plants, then selecting a mist nozzle pot to spray thin water (the condition that the seedlings are damaged due to too large water drops or too much watering) to more than 90%, and completely covering the seedling tray with a colorless plastic cover in a short time after watering is completed to avoid contact with the outside. Culturing the transplanted seedlings to grow under the environment that the culture temperature is 22 +/-2 ℃, the illumination intensity is 2000-2500 LX, and the illumination time is 12 h;
b. managing the transplanted seedlings: uncovering the plastic cover after transplanting the tissue culture seedlings for 6-7 days, shaking off a large amount of water drops on the plastic cover and avoiding falling into the seedling tray, ventilating the seedling tray for 30 minutes, spraying a small amount of water when the growth state of the transplanted seedlings is as shown in figure 1, spraying 1500 plus water for 2000 milliliters per tray, then covering the seedling tray again by the plastic cover, reserving a gap of more than 2cm at the short edge of the seedling tray during covering so as to facilitate air circulation, enabling the transplanted seedlings to gradually adapt to the natural environment, carefully observing the morphology of the transplanted seedlings in the stage, spraying water once in 2-3 days, growing new tips and milky new roots after 12-15 days, and completely uncovering the plastic cover when the transplanted seedlings enter the autotrophic stage from the morphology so as to perform conventional growth management on the transplanted seedlings;
c. strong seedling culture of the transplanted seedlings: growing the transplanted seedlings with new stem tips and new roots for growth management, and spraying water once every 3 days, wherein the water spraying amount is 2000-2500 ml; uniformly spreading a granular compound fertilizer after 15 days, applying the fertilizer for 1 time every 10 days, and applying 5 g/disc of the primary fertilizer; 7 g for the second time and 10g for the third time; meanwhile, in order to avoid propagation, spreading and growth of fungi, spraying carbendazim once every 7-10 days, wherein the concentration of the carbendazim is 800-1000 times; after the transplanted seedlings grow for 30-40 days, the height of the transplanted seedlings reaches 15-20 cm, the roots of the transplanted seedlings are developed, the stems are thick and strong, the leaves are dark green, the plants are strong, and the growth states of the plants are shown in figure 2, so that the transplanted seedlings can be transplanted into a nutrition pot.
The operation is carried out, 12 disks of seedlings are transplanted at one time, the transplanting survival rate is more than 92%, and the transplanting survival rate of the conventional tissue culture seedlings is only about 75% after the seedling hardening stage; compared with the prior art, the transplanting method is simple, and the survival rate is remarkably improved.
Example 1.2
The transplanting method of the fraxinus chinensis tissue culture seedlings comprises the following steps:
a. transplanting the tissue culture seedlings:
a1, selecting a transplanting container: adopting a standard plastic seedling tray without volatile smell, wherein the length of the seedling tray is 53cm, the width of the seedling tray is 28cm, and the height of the seedling tray is 5cm, and the seedling tray is portable and practical;
a2, selecting a substrate: the matrix suitable for planting the tissue culture seedlings has air permeability, moisture retention and certain fertility, an organic matrix-vermiculite (new vermiculite) is selected, the standard of the vermiculite is 1-3 mm, the vermiculite loading amount of each seedling tray is 4.5-5 cm, the matrix is too little to be beneficial to the growth and moisture retention of the tissue culture seedlings, and then the matrix is sprayed until the water content of the matrix is more than 70% to prepare for transplanting the tissue culture seedlings;
a3, cleaning the tissue culture seedlings before transplanting: selecting a robust tissue culture seedling with more than 3 roots (the roots need to be provided with root hairs), removing a sealing film on a culture bottle, immediately and lightly taking out the seedling from the culture bottle by using tweezers to avoid the influence on the survival rate caused by withering of the tissue culture seedling, lightly washing off a culture medium at the root base of the tissue culture seedling by using clear water, not damaging young roots and root hairs, and refreshing for 2-3 times;
a4, transplanting operation: transplanting clean tissue culture seedlings into a seedling tray with the substrate water content of more than 70%, digging holes by using a seedling planting shovel during transplanting, placing one tissue culture seedling in each hole, gently placing and compacting the tissue culture seedlings, extending roots of the transplanted seedlings, enabling root systems to be grouped together to be unfavorable for seedling reviving and growth, enabling the standard of each tray to be 50 plants, then selecting a mist nozzle kettle to spray thin water (the thin water means no water drop, looks like mist, and can avoid the situation that the seedlings are damaged due to too large water drop or too much watering), enabling the seedling tray to be completely covered with a colorless plastic cover in a short time after watering is completed, and avoiding external contact. Placing the mixture in an environment with a culture temperature of 22 +/-2 ℃, an illumination intensity of 2000-2500 LX and an illumination time of 12h for continuous growth;
b. managing the transplanted seedlings: uncovering the plastic cover after the tissue culture seedlings are transplanted for 6-7 days, shaking off a large number of water drops on the plastic cover and avoiding falling into the seedling tray, ventilating the seedling tray for 50 minutes, spraying 2000 milliliters of water according to the content of the matrix, then covering the seedling tray with the plastic cover again, reserving a 3cm gap at the short edge of the seedling tray during covering so as to facilitate air circulation, enabling the tissue culture transplanted seedlings to gradually adapt to the natural environment, carefully observing the shape of the transplanted seedlings at the stage, replenishing water once for 3 days, growing new tips and milky new roots of the transplanted seedlings after 12-15 days, completely uncovering the plastic cover, and performing conventional growth management on the transplanted seedlings;
c. strong seedling culture of the transplanted seedlings: growing the transplanted seedlings with new stem tips and new roots for growth management, and spraying water once every 3 days, wherein the water spraying amount is 2000-2500 ml; uniformly spreading a granular compound fertilizer after 15 days, applying the fertilizer for 1 time every 10 days, and applying 5 g/disc of the primary fertilizer; 7 g for the second time and 10g for the third time; meanwhile, in order to avoid the propagation and the spreading growth of fungi, the carbendazim is sprayed once every 7 to 10 days, and the concentration of the carbendazim is 800-1000 times; after the transplanted seedlings grow for 30-40 days, the height of the transplanted seedlings reaches 15-20 cm, the roots of the transplanted seedlings are developed, the stems are thick and strong, the leaves are thick and green, and the plants are robust and can be transplanted into a nutrition pot.
The operation is carried out, 10 disks of transplanting are carried out at one time, and the transplanting survival rate reaches 95.5 percent; the conventional tissue culture seedling transplantation needs a seedling hardening stage, and the transplantation survival rate is only 75%; compared with the prior art, the transplanting method is simple, and the survival rate is remarkably improved by more than 20%.
And c, when the seedling tray is covered by the plastic cover again in the step b, the size of the remained gap needs to be strictly controlled, if the gap is smaller than 2cm, the circulation of air and the outside is not facilitated, the air humidity is high, and the phenomenon that the small seedlings without new roots are easy to rot and die gradually is caused, if the gap is larger than 3cm, the tissue culture transplanted seedlings do not grow new growing points and new roots and do not enter an autotrophic stage due to short transplanting time, and are suddenly influenced by a dry external environment, so that the dry tips and wilting of the transplanted seedlings are easy to occur, and the growth or survival rate of the transplanted seedlings is seriously influenced.
Comparative example
The embodiment relates to the influence of the operation of covering a seedling tray with a plastic cover in the tissue culture seedling transplanting method on the transplanting survival rate. The following transplanting method is adopted:
a. transplanting the tissue culture seedlings: the same operation steps as the step a of the embodiment are adopted;
b. managing the transplanted seedlings: uncovering the plastic cover after the tissue culture seedlings are transplanted for 6-7 days, shaking off a large amount of water drops on the plastic cover and avoiding falling into the seedling tray, ventilating the seedling tray for 30 minutes, spraying 2000 milliliters of water according to the content of the matrix, then completely covering the seedling tray with the plastic cover again, directly opening the plastic cover after 10 days to enable the transplanted seedlings to gradually adapt to the natural environment, slightly spraying water once after 3 days in the stage, slightly spraying water once after 12-15 days, and observing that the transplanted seedlings grow new tips and white new roots from morphological characteristics, wherein part of the transplanted seedlings die;
c. strong seedling culture of the transplanted seedlings: the same procedure as in step c of example; after the transplanted seedlings grow for 30-40 days, the height of the transplanted seedlings reaches 10-15 cm, the height and the height of the transplanted seedlings are different, the seedlings are thin and weak, 5 disks of seedlings are transplanted, the survival rate of one disk is only 25%, the statistical transplanting survival rate is 25-75%, and the statistics is not ideal, so that the success of dozens of procedures is abandoned.
Compared with the comparative example, the transplanting survival rate of the fraxinus chinensis tissue culture seedlings can be obviously improved by the transplanting method.
In conclusion, the tissue culture seedling transplanting method provided by the invention realizes that the tissue culture seedlings are directly transplanted and strictly managed without seedling hardening treatment, so that the transplanting survival rate is improved; the transplanting operation process is greatly simplified and standardized, factors influencing survival of tissue culture seedlings in the transplanting process are reduced, the transplanting operation is more stable and easy to control, the transplanting survival rate of the fraxinus chinensis tissue culture seedlings can be remarkably improved to be more than 90%, support is provided for healthy growth of the transplanted fraxinus chinensis plants, an effective technical approach is provided for preservation and application of high-quality tissue culture germplasm resources, and the method has remarkable popularization significance.
Claims (8)
1. A tissue culture seedling transplanting method for woody plant fraxinus chinensis is characterized in that: the method comprises the following operation steps:
a. transplanting the tissue culture seedlings: transplanting the cleaned tissue culture seedlings into a seedling tray filled with a matrix, spraying thin water until the water content of the matrix is more than 90 percent after the transplanting is finished, then completely covering the seedling tray with a plastic cover, and culturing the transplanted seedlings to grow under the environment of the culture temperature of 22 +/-2 ℃, the illumination intensity of 2000-2500 LX and the illumination time of 12 h;
b. managing the transplanted seedlings: uncovering the plastic cover after the tissue culture seedlings are transplanted for 6-7 days, shaking off water drops on the plastic cover and avoiding falling into the seedling tray, ventilating the seedling tray for 30-50 minutes, then covering the seedling tray with the plastic cover again, reserving a gap of 2-3 cm at the short edge of the seedling tray during covering, completely uncovering the plastic cover when the transplanted seedlings grow new stem tips and new roots after the transplanted seedlings grow for 12-15 days, and performing conventional growth management on the transplanted seedlings;
c. strong seedling culture of the transplanted seedlings: and (3) carrying out growth management on the transplanted seedlings with new stem tips and new roots, spraying water once every 3-4 days, uniformly spreading compound fertilizer and carbendazim after 15 days, applying fertilizer for 1 time every 7-10 days, spraying carbendazim once every 7-10 days, and transplanting the seedlings into a nutrition pot after the transplanted seedlings grow for 30-40 days, wherein the plants of the transplanted seedlings are dark green and robust, and are as high as 15-20 cm.
2. The method for transplanting tissue culture seedlings of woody plant fraxinus chinensis as claimed in claim 1, wherein the requirements for cleaning the tissue culture seedlings before transplantation in step a are as follows:
selecting robust tissue culture seedlings with more than 3 roots, immediately taking out the robust tissue culture seedlings from a culture container without damaging or bumping the roots, slightly washing off a culture medium at the root base part by using clear water, and washing for 2-3 times.
3. The method for transplanting tissue culture seedlings of a woody plant Fraxinus chinensis as claimed in claim 1, wherein: step a, vermiculite is selected as the substrate, the vermiculite loading amount of each seedling tray reaches 4-5 cm, and water is sprayed until the water content of the substrate is more than 70% for transplanting tissue culture seedlings; after the transplanting is finished, thin water is continuously sprayed until the water content of the matrix is more than 90 percent.
4. The method for transplanting tissue culture seedlings of a woody plant Fraxinus chinensis as claimed in claim 1, wherein: and a standard plastic seedling tray with the length of 53cm, the width of 28cm and the height of 5cm is selected as the seedling tray in the step a.
5. The method for transplanting tissue culture seedlings of a woody plant Fraxinus chinensis as claimed in claim 4, wherein: and b, transplanting 50 tissue culture seedlings in the seedling tray.
6. The method for transplanting tissue culture seedlings of a woody plant Fraxinus chinensis as claimed in claim 1, wherein: and c, after the seedling tray is ventilated and before the plastic cover is covered again, replenishing water to the seedling tray.
7. The method for transplanting tissue culture seedlings of a woody plant Fraxinus chinensis as claimed in claim 1, wherein: and c, in the culture stage of the seedling tray covered with the plastic cover again, according to the transplanted seedling form, supplementing water to the seedling tray every 2-3 days.
8. The method for transplanting tissue culture seedlings of a woody plant Fraxinus chinensis as claimed in claim 1, wherein: and c, selecting a granular compound fertilizer as the compound fertilizer, wherein the fertilizing amount is 5-10 g/disc.
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| CN115804315A (en) * | 2022-12-28 | 2023-03-17 | 中国科学院遗传与发育生物学研究所农业资源研究中心 | Method for managing secondary forest close to natural forest by utilizing natural forest gap |
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| CN1288656A (en) * | 2000-11-09 | 2001-03-28 | 中国科学院石家庄农业现代化研究所 | Fast seedling growing mehtod for Chinese flowering crabapple zhumei |
| CN101116422A (en) * | 2006-08-02 | 2008-02-06 | 中国科学院遗传与发育生物学研究所 | Root culture medium and the culture method in the purple leaf oxalis |
| CN104381128A (en) * | 2014-10-15 | 2015-03-04 | 山东省林业科学研究院 | Method for improving tissue culture and rapid propagation proliferation rate of Fraxinus velutina |
| CN104396759A (en) * | 2014-12-10 | 2015-03-11 | 中国科学院遗传与发育生物学研究所 | Method for tissue culture and rapid breeding of Fraxinus chinensis |
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| CN1288656A (en) * | 2000-11-09 | 2001-03-28 | 中国科学院石家庄农业现代化研究所 | Fast seedling growing mehtod for Chinese flowering crabapple zhumei |
| CN101116422A (en) * | 2006-08-02 | 2008-02-06 | 中国科学院遗传与发育生物学研究所 | Root culture medium and the culture method in the purple leaf oxalis |
| CN104381128A (en) * | 2014-10-15 | 2015-03-04 | 山东省林业科学研究院 | Method for improving tissue culture and rapid propagation proliferation rate of Fraxinus velutina |
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