CN1288656A - Fast seedling growing mehtod for Chinese flowering crabapple zhumei - Google Patents
Fast seedling growing mehtod for Chinese flowering crabapple zhumei Download PDFInfo
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- CN1288656A CN1288656A CN 00133497 CN00133497A CN1288656A CN 1288656 A CN1288656 A CN 1288656A CN 00133497 CN00133497 CN 00133497 CN 00133497 A CN00133497 A CN 00133497A CN 1288656 A CN1288656 A CN 1288656A
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Abstract
The method for quickly-cultivating seedling of malus spectabilis includes the following steps: disinfecting annual sapling top bud and side bud; inoculating on the inductive culture medium to make induction, differentiation and culture; inoculating growing bud on the enrichment culture medium from stem apex; light and dark alternatively culturing, differentiating yellowing bud group from stem apex, cutting off and making successive transfer light culture for one month to make budlet reproduce. Said invention possesses the advantages of high survival rate and quick reproduction, as compared with conventional light culture its reproduction rate can be raised by twice, so it is suitable for industrial production.
Description
The present invention relates to the method for the U.S. Malus spectabilis of a kind of salt tolerant alkali wild fruit tree variety source pearl (Mulus zumi) group training rapid propagation in factory.
The U.S. Malus spectabilis of pearl is the wilding stock variety resource of drawing from external purport, because of finding it resistance such as stronger salt tolerant alkali, waterlogging low-lying area and mildew-resistance is arranged, and the directive breeding through domestic ten years has obtained the U.S. Malus spectabilis kind of anti-saline and alkaline pearl.These seeds are dungarunga, can yield positive results, and the tree type is attractive in appearance, are greening, economic dual-purpose type seeds, are suitable for applying in China salinization soil area.Because provenance lacks, the cuttage difficulty is taken root and genetic variation etc. is all multifactor, the U.S. Malus spectabilis of pearl is difficult to obtain in a short time in batches nursery stock for applying by conventional propagation method.
The objective of the invention is to disclose the U.S. Malus spectabilis seedling-cultivating method of a kind of pearl, for the extensive fast breeding of batch production provides the fine individual plant seedling.
Zhumeitang group training fast seedling-cultivating method of the present invention is finished by following step:
A, with annual treelet terminal bud or lateral bud, be seeded in after the sterilization and induce differentiation culture on the inducing culture, temperature 23-27 ℃, light intensity 2000Lux cultivated 10 days;
B, the bud clump stem apex of will growing are inoculated on the proliferated culture medium, and temperature 23-27 ℃, light intensity 500-1000Lux, one week of illumination cultivation, change dark two weeks of cultivation over to, the bud clump segment illumination cultivation of follow-up generation that stem apex differentiates yellow made seedling and bud proliferation in one month;
C, the seedling of will taking root are directly planted in the vermiculite seedling-cultivating tray, and film covers and preserves moisture, and puts into green house and cultivates, and temperature 20-30 ℃, light intensity 2000-5000Lux opens the ventilation striping gradually after 10 days,
D, with the seedling of transplant survival at illumination 1500-3000Lux, temperature 19-30 ℃, under the greenhouse of relative moisture 60-80% seedling is sprayed N, P, K, Mg nutrient solution and CO
2Gas fertilizer is cultivated speed and is given birth to commercial seedling.
The composition of the described inducing culture of Zhumeitang group training fast seedling-cultivating method comprises MS medium 4.0-4.5g/L, 6-BA (6-Bian Ji purine) 0.5mg/L, IAA (Yin tremble acetate) 0.2mg/L.
The composition of the described proliferated culture medium of Zhumeitang group training fast seedling-cultivating method comprises MS medium 4.0-4.5g/L, 6-BA (6-Bian Ji purine) 0.5mg/L, IAA (Yin tremble acetate) 0.2mg/L, sucrose 30g/L.
Advantage of the present invention is the survival rate of seedling height, and propagation is fast, cultivates than conventional light and improves 2 times of the rates of increase, is fit to very much batch production production.
Describe technology contents of the present invention in detail below in conjunction with embodiment:
Test-tube plantlet batch production production example:
A, explant are made and are just cultivated
Choose terminal bud or the lateral bud of annual fine individual plant from the nursery, clean surperficial thing, sterilized 3-5 minute with 70% alcohol and antiformin sterilization 10 minutes or with 0.1% HgCl, aseptic water washing 3-4 time, suck dry moisture, peel off scale, simple bud be seeded in induce differentiation culture on the inducing culture, the consisting of of inducing culture:
MS (Marashige ﹠amp; The Shoog medium is hereinafter to be referred as the MS medium) 4.1g/L
6-BA (6-Bian Ji purine) 0.5mg/L
IAA (heteroauxin) 0.2mg/L
B, yellow are handled subculture and are expanded numerous cultivation
Clip is just cultivated the bud clump stem apex of coming out, and is inoculated on the proliferated culture medium, cultivates a week under light intensity 500-1000Lux condition, transfers dark two weeks of cultivation again to, and the stem apex segment is seeded on the proliferated culture medium, can cultivate into a collection of high-quality and not have the radical bud seedling in 30 days.Cultivate stem apex at the beginning of one, can breed 80-120 bud seedling in 50 days, cultivate with conventional light and improve 2 times of the rates of increase through yellow alternation of light and darkness cultivation.The composition of proliferated culture medium is
MS medium 4.5g/L
6-BA (6-Bian Ji purine) 0.5mg/L
IAA (heteroauxin) 0.2mg/L
Sucrose 30g/L
C, the transplantation of seedlings of taking root
A small amount of vitro rooting in test tube seedling need not hardening after the uncork and directly plants in the sterilization vermiculite, covers with film and preserves moisture, place greenhouse, booth or open-air rearing, illumination 2000-5000Lux, temperature 20-30 ℃, open after 10 days 1/5 coverlay fall wet, striping after 20 days, transplanting survival rate is more than 90%.
D, test tube transplanted seedling speed are given birth to and are cultivated
It is 0.1% main nutrient solution that preparation contains N, P, K, Mg, carries out root and top dressing in per 10 days.In confined conditions, with 500-800ppm steel cylinder CO is arranged during 9-10 the fine morning
2Gas carries out CO
2Fertilising, continuous fertilization 25 days was cultivated into the conforming articls seedling after 50 days.
The U.S. Malus spectabilis group of pearl training factorial seedling-culturing method key points for operation are as follows:
1, the maternal plant material of good, the eugonic individual plant work of screening genetic character group training factorial seedling growth production: the U.S. Malus spectabilis of pearl is a heterozygote, its offspring easily morphs and separates degradation phenomena, therefore, group training material to constantly choose fine individual plant seedling material with substitution number for expanding numerous subculture material that may produce degeneration, thereby guarantee the stability of the U.S. Malus spectabilis seedling quality of pearl.
2, the yellow of differentiation seedling is handled: for improving the rate of increase, obtain more bud seedling as propagation material, adopt the alternation of light and darkness cultivation.Concrete operations: will just cultivate inoculating stem tip to the propagation base, in one week of illumination cultivation, light intensity is 500-1000Lux, after change dark two weeks of cultivation over to, stem apex differentiates the bud clump of cluster yellow, and yellow bud seedling segment light of follow-up generation was cultivated one month, can turn out large quantities of high-quality no radical bud seedlings.Cultivate stem apex at the beginning of one by the alternation of light and darkness cultivation, can breed 80-120 bud seedling by means of culture of rootage usefulness in 50 days, the rate of increase of cultivating than conventional light improves 2 times.
3, test-tube seedling transplanting
The tradition method for transplanting is the test tube bottle to be opened placement transplant after 2-3 days again, promptly transplants after the hardening.The present invention adopts not the hardening film to preserve moisture and transplants the seedling of taking root in batches.Ship from the test-tube plantlet workshop, need not the uncork hardening but directly will take root transplantation of seedlings in the sterilization vermiculite of Miao Panzhong, preserve moisture with the film covering, put into the greenhouse or booth is cultivated, about light intensity 2000-5000Lux, temperature 20-30 ℃, open ventilation gradually after 10 days, whole stripings after 15 days, transplanting survival rate is more than 90%.The acclimatization and transplants method can not saved a large amount of artificial and operating spaces, reduces the factorial seedling growth cost.
4, transplanted seedling speed is given birth to and is cultivated: with the seedling of large batch of transplant survival, be placed on the seedling dish on the three-dimensional seedling raising frame of greenhouse or booth, regulate indoor environment, making its illumination is 1500-3000Lux, temperature 19-30 ℃, relative moisture 60-80%, executed once based on the nutrient solution of N, P, K, Mg to seedling in per 10 days, concentration is 0.1%.Under the environment airtight condition, utilize steel cylinder CO in addition
2Gas is that seedling carries out CO
2500-800ppm concentration C O is used in fertilising during fine day 9-10 in the morning
2Carry out the air fertilising, growth of seedling is vigorous after continuous 25 days, and more than the high 10cm of seedling, the stem stalk is sturdy after 50 days for the living cultivation of speed, and well developed root system becomes the commodity seedling, can be for nursery field planting between spring and autumn and double-crop field.
Claims (3)
1, a kind of Zhumeitang group training fast seedling-cultivating method is characterized in that:
A, with annual treelet terminal bud or lateral bud, be seeded in after the sterilization and induce differentiation culture on the inducing culture, temperature 23-27 ℃, light intensity 2000Lux cultivated 10 days;
B, the bud clump stem apex of will growing are inoculated on the proliferated culture medium, and temperature 23-27 ℃, light intensity 500-1000Lux, one week of illumination cultivation, change dark two weeks of cultivation over to, the bud clump segment illumination cultivation of follow-up generation that stem apex differentiates yellow made seedling and bud proliferation in one month;
C, the seedling of will taking root are directly planted in the vermiculite seedling-cultivating tray, and film covers and preserves moisture, and puts into green house and cultivates, and temperature 20-30 ℃, light intensity 2000-5000Lux opens the ventilation striping gradually after 10 days,
D, with the seedling of transplant survival at illumination 1500-3000Lux, temperature 19-30 ℃, under the greenhouse of relative moisture 60-80% seedling is sprayed N, P, K, Mg nutrient solution and CO
2Gas fertilizer is cultivated speed and is given birth to commercial seedling.
2, Zhumeitang group according to claim 1 training fast seedling-cultivating method is characterized in that the composition of described inducing culture comprises MS medium 4.0-4.5g/L, 6-BA (6-Bian Ji purine) 0.5mg/L, IAA (Yin tremble acetate) 0.2mg/L.
3, Zhumeitang group according to claim 1 training fast seedling-cultivating method is characterized in that the composition of described proliferated culture medium comprises MS medium 4.0-4.5g/L, 6-BA (6-Bian Ji purine) 0.5mg/L, IAA (Yin tremble acetate) 0.2mg/L, sucrose 30g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00133497 CN1110250C (en) | 2000-11-09 | 2000-11-09 | Fast seedling growing mehtod for Chinese flowering crabapple zhumei |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00133497 CN1110250C (en) | 2000-11-09 | 2000-11-09 | Fast seedling growing mehtod for Chinese flowering crabapple zhumei |
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| Publication Number | Publication Date |
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| CN1288656A true CN1288656A (en) | 2001-03-28 |
| CN1110250C CN1110250C (en) | 2003-06-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 00133497 Expired - Fee Related CN1110250C (en) | 2000-11-09 | 2000-11-09 | Fast seedling growing mehtod for Chinese flowering crabapple zhumei |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696487A (en) * | 2012-07-05 | 2012-10-03 | 江苏省中国科学院植物研究所 | Method for building leaf in vitro regeneration system of begonia rex |
| CN103155866A (en) * | 2011-12-10 | 2013-06-19 | 天水市果树研究所 | Malus zumi tissue culture rapid propagation seedling raising method |
| CN107047217A (en) * | 2017-06-09 | 2017-08-18 | 中国科学院遗传与发育生物学研究所农业资源研究中心 | The tissue culture method for transplanting of xylophyta Chinese wax |
| CN108040885A (en) * | 2018-01-29 | 2018-05-18 | 宝鸡松良农业科技有限公司 | A kind of method that tissue cultures are carried out using cherry stem section |
| CN108124773A (en) * | 2018-01-29 | 2018-06-08 | 宝鸡松良农业科技有限公司 | A kind of method that tissue cultures are carried out using apple stem section |
| CN108849498A (en) * | 2018-04-27 | 2018-11-23 | 北京农学院 | A method of promoting the accumulation of admiring fruit tree leaf tissue flavonoid substances |
-
2000
- 2000-11-09 CN CN 00133497 patent/CN1110250C/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103155866A (en) * | 2011-12-10 | 2013-06-19 | 天水市果树研究所 | Malus zumi tissue culture rapid propagation seedling raising method |
| CN103155866B (en) * | 2011-12-10 | 2015-06-17 | 天水市果树研究所 | Malus zumi tissue culture rapid propagation seedling raising method |
| CN102696487A (en) * | 2012-07-05 | 2012-10-03 | 江苏省中国科学院植物研究所 | Method for building leaf in vitro regeneration system of begonia rex |
| CN102696487B (en) * | 2012-07-05 | 2013-08-07 | 江苏省中国科学院植物研究所 | Method for building leaf in vitro regeneration system of begonia rex |
| CN107047217A (en) * | 2017-06-09 | 2017-08-18 | 中国科学院遗传与发育生物学研究所农业资源研究中心 | The tissue culture method for transplanting of xylophyta Chinese wax |
| CN107047217B (en) * | 2017-06-09 | 2020-05-26 | 中国科学院遗传与发育生物学研究所农业资源研究中心 | Tissue culture seedling transplanting method for woody plant fraxinus chinensis |
| CN108040885A (en) * | 2018-01-29 | 2018-05-18 | 宝鸡松良农业科技有限公司 | A kind of method that tissue cultures are carried out using cherry stem section |
| CN108124773A (en) * | 2018-01-29 | 2018-06-08 | 宝鸡松良农业科技有限公司 | A kind of method that tissue cultures are carried out using apple stem section |
| CN108849498A (en) * | 2018-04-27 | 2018-11-23 | 北京农学院 | A method of promoting the accumulation of admiring fruit tree leaf tissue flavonoid substances |
| CN108849498B (en) * | 2018-04-27 | 2022-03-08 | 北京农学院 | Method for promoting accumulation of flavonoid substances in leaf tissues of ornamental crabapple |
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| Publication number | Publication date |
|---|---|
| CN1110250C (en) | 2003-06-04 |
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