CN107231940A - Ferfas is inoculated with bacterium ball and its preparation and application - Google Patents
Ferfas is inoculated with bacterium ball and its preparation and application Download PDFInfo
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- CN107231940A CN107231940A CN201710413126.5A CN201710413126A CN107231940A CN 107231940 A CN107231940 A CN 107231940A CN 201710413126 A CN201710413126 A CN 201710413126A CN 107231940 A CN107231940 A CN 107231940A
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- ferfas
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
Abstract
The invention belongs to ferfas planting technology field, specifically related to a kind of ferfas inoculation bacterium ball and its preparation and application.For the ferfas bacterium ball holding time is short, inoculation rate is inconsistent and in-convenience in use the problems such as, the present invention provides a kind of ferfas inoculation bacterium ball and its preparation and application.Ferfas inoculation bacterium ball composition of the present invention includes：30~35 parts of ferfas powder, 8~12 parts of limestone powder, 20~25 parts of vermiculite power, 10~20 parts of peat dust, 1~2 part of EM saccharomycete, 4~6 parts of white sugar, 1~2 part of bacillus subtilis.Above-mentioned raw materials are weighed up in proportion, stirred and evenly mixed in 1000~1200 turns/min, the bacterium ball of weight 1.5~2g/ is pressed into using tablet press machine, are packed after drying, ferfas bacterium ball is made.The ferfas inoculation bacterium ball of the present invention, holding time length, easy to use and inoculation rate are high, shorten the time of mycorhiza formation, increase substantially plant fibrous root quantity, be worth of widely use.
Description
Technical field
The invention belongs to ferfas planting technology field, specifically related to a kind of ferfas inoculation bacterium ball and its preparation and user
Method.
Background technology
Ferfas (T.ruffles) is ascomycetes Tuberale (Tuberales) Tuberaceae (Tuberaceae) Tuber
(Tuber) it is, symbiosis edible fungus most precious in the world, with peculiar fragrance and nutritive value, therefore limits throughput, state
Border market at prices is expensive, and supply falls short of demand, wherein be described as " black diamond " again.
In order to improve the yield of block mycorrhiza fungi seeding cultivating quality and wild ferfas, existing resource is effectively protected, block is realized
Bacterium sustainable utilization, generally cultivates truffle mycorrhiza fungi seeding using ferfas bacterium ball inoculation host plant, so as to carry out artificial cultivation, can also lead to
Cross the wild ferfas yield that the mode reseeded on the field bacterium pool improves the bacterium pool.The growth of ferfas must be with suitable host plant root system
Commensalism is sought, otherwise will be unable to form ascocarp, this means that artificial cultivation ferfas be unable to do without live body host plant, thus
Ferfas artificial cultivation is relatively difficult, with high content of technology.
External inoculation is cultivated Va Mycorrhiza Seedling and is mainly inoculated with using liquid bacteria ball and solid bacterium ball at present, and the inoculation of solid bacterium ball is logical
Frequently with sterile bare-root seeding is first cultivated, nutritive cube is changed during inoculation, the shortcoming inoculation seedling death rate of this inoculation method is high, inoculation
Seedling-slowing stage for some time, influences seedling growth afterwards.Liquid bacteria ball inoculation it is uneven, ferfas spore have it is many, have lack, after inoculation
The quality for forming mycorhiza is inconsistent, or even the few infected plant that is unable to of inoculation forms mycorhiza, and the liquid spawn holding time is very short,
General no more than 7 days.
The content of the invention
The technical problem to be solved in the present invention is：The inoculation seedling death rate is high, the ferfas fungi preservation time is short, inoculation rate
It is inconsistent and in-convenience in use the problems such as.
The present invention solve technical problem technical scheme be：A kind of ferfas inoculation bacterium ball and its preparation and user are provided
Method.
The present invention provides a kind of ferfas inoculation bacterium ball, and its composition includes：Count by weight, 30~35 parts of ferfas powder,
8~12 parts of limestone powder, 20~25 parts of vermiculite power, 10~20 parts of peat dust, 1~2 part of EM saccharomycete, 4~6 parts of white sugar is withered
Careless 1~2 part of bacillus.
It is preferred that, above-mentioned ferfas is inoculated with bacterium ball, and its composition includes：Count by weight, 33 parts of ferfas powder, agstone
10 parts of end, 23 parts of vermiculite power, 15 parts of peat dust, 2 parts of EM saccharomycete, 5 parts of white sugar, 2 parts of bacillus subtilis.
Wherein, in above-mentioned ferfas inoculation bacterium ball, described limestone powder, vermiculite power, peat dust particle diameter≤
0.5mm。
Wherein, in above-mentioned ferfas inoculation bacterium ball, described ferfas is India truffle, summer truffle, Panzhihua ferfas, Podbielniak block
One kind in bacterium, black truffle, wealthy spore ferfas or Li Yu ferfas.
The present invention also provides the preparation method that a kind of above-mentioned ferfas is inoculated with bacterium ball, comprises the following steps：
A, ferfas strain collection and preservation
Ripe ferfas is chosen, diameter≤0.5mm powder is ground into after sterilizing；
B, preparation ferfas bacterium ball
By 30~35 parts of ferfas powder, 8~12 parts of limestone powder, 20~25 parts of vermiculite power, 10~15 parts of peat dust, EM
1~2 part of saccharomycete, 4~6 parts of white sugar, 1~2 part of bacillus subtilis weighs above-mentioned raw materials, in 1000~1200 turns/min stirrings
Mix, the bacterium ball of weight 1.5~2g/ is pressed into using tablet press machine, packed after drying, ferfas bacterium ball is made.
Wherein, in the preparation method of above-mentioned ferfas inoculation bacterium ball, the ripe ferfas described in step a refers to the maturation of spore
Spend ＞ 95% ferfas；The maturity of the spore refers to that spore count ripe under microscope accounts for the percentage of total spore count.
Wherein, in the preparation method of above-mentioned ferfas inoculation bacterium ball, the method for the sterilizing described in step a is：With 70~
75% alcohol-pickled 2~3min.
Wherein, in the preparation method of above-mentioned ferfas inoculation bacterium ball, temperature≤35 DEG C when being suppressed described in step b.
Wherein, in the preparation method of above-mentioned ferfas inoculation bacterium ball, the drying described in step b is vacuum drying or dried in the shade,
Vacuum drying temperature is 30~35 DEG C, 0.06~0.08MPa of vacuum.
Wherein, in the preparation method of above-mentioned ferfas inoculation bacterium ball, dried bacterium ball water content described in step b is 9~
12%.
The present invention also provides the application method that a kind of above-mentioned ferfas is inoculated with bacterium ball, and inoculum concentration is 2~10/plant, and inoculation is deep
Spend for away from native 5~10cm of surface.
Beneficial effects of the present invention are：The present invention provides a kind of ferfas inoculation bacterium ball, can improve mycorhiza culture matrix and micro-
Biological structure, increases substantially plant fibrous root quantity, infection rate can be improved into 20% or so.Can also shorten mycorhiza formation when
Between, the time using bacterium ball of the present invention than conventional bacterium ball formation mycorhiza shortens more than 2 months.The bacterium ball of the present invention is prepared and used
Method is simple, can be inoculated with for cultivating aseptic seedling, the volume increase of artificial cultivation is inoculated with, the volume increase inoculation of the wild ferfas bacterium pool provides basis,
It is worth of widely use.
Embodiment
The present invention provides a kind of ferfas inoculation bacterium ball, and its composition includes：Count by weight, 30~35 parts of ferfas powder,
8~12 parts of limestone powder, 20~25 parts of vermiculite power, 10~15 parts of peat dust, 1~2 part of EM saccharomycete, 4~6 parts of white sugar is withered
Careless 1~2 part of bacillus.
It is preferred that, above-mentioned ferfas is inoculated with bacterium ball, and its composition includes：Count by weight, 33 parts of ferfas powder, agstone
10 parts of end, 23 parts of vermiculite power, 15 parts of peat dust, 2 parts of EM saccharomycete, 5 parts of white sugar, 2 parts of bacillus subtilis.
Wherein, in above-mentioned ferfas inoculation bacterium ball, described limestone powder, vermiculite power, peat dust particle diameter≤
0.5mm。
Present invention selection limestone powder, vermiculite power, peat dust and EM saccharomycete and Panzhihua ferfas use cooperatively, lime
Stone powder can make strain keep drying, moreover it is possible to provide the calcareous of ferfas growth, adjust pH value；Vermiculite power can maintain bacterium ball
Gas porosity, plays filling effect, moreover it is possible to supplement mineral matter；Peat dust contains humic acid, can promote ferfas spore germination, promotes to plant
Thing root growth；EM saccharomycete and bacillus subtilis can improve the microorganism structure of culture matrix, promote the formation of mycorhiza；
White sugar can supplement nitrogen source, and with adhesive effect；Above-mentioned substance is used cooperatively so that ferfas inoculation bacterium ball nutrition is rich
Richness, inoculation rate is high, moreover it is possible to effectively improve fibrous root quantity.
Wherein, in above-mentioned ferfas inoculation bacterium ball, described ferfas is India truffle, summer truffle, Panzhihua ferfas, Podbielniak block
One kind in bacterium, black truffle, wealthy spore ferfas or Li Yu ferfas.
The present invention also provides the preparation method that a kind of above-mentioned ferfas is inoculated with bacterium ball, comprises the following steps：
A, ferfas strain collection and preservation
Ripe ferfas is chosen, diameter≤0.5mm powder is ground into after sterilizing；
B, preparation ferfas bacterium ball
By 30~35 parts of ferfas powder, 8~12 parts of limestone powder, 20~25 parts of vermiculite power, 10~15 parts of peat dust, EM
1~2 part of saccharomycete, 4~6 parts of white sugar, 1~2 part of bacillus subtilis weighs raw material, is positioned over 1000~1200 turns/min stirrings
Stirred 1~2 minute in machine, after being sufficiently mixed uniformly, then the bacterium ball or bacterium piece for using tablet press machine to be pressed into weight 1.5~2g/, very
Sky drying is packed, and ferfas bacterium ball is made.
Wherein, in the preparation method of above-mentioned ferfas inoculation bacterium ball, the ripe ferfas described in step a refers to the maturation of spore
Spend ＞ 95% ferfas；The maturity of the spore refers to that spore count ripe under microscope accounts for the percentage of total spore count.
Wherein, in the preparation method of above-mentioned ferfas inoculation bacterium ball, the method for the sterilizing described in step a is：With 70~
75% alcohol-pickled 2~3min.
Wherein, in the preparation method of above-mentioned ferfas inoculation bacterium ball, temperature≤35 DEG C when being suppressed described in step b.
Wherein, in the preparation method of above-mentioned ferfas inoculation bacterium ball, the drying described in step b is vacuum drying or dried in the shade,
Vacuum drying temperature is 30~35 DEG C, 0.06~0.08MPa of vacuum.
Wherein, in the preparation method of above-mentioned ferfas inoculation bacterium ball, in order that the effective preservation of bacterium ball, and ensure ferfas spore
Sprouting ability, dried bacterium ball water content described in step b of the present invention is 9~12%.
The present invention also provides the application method that a kind of above-mentioned ferfas is inoculated with bacterium ball, and inoculum concentration is 2~10/plant, and inoculation is deep
Spend for away from native 5~10cm of surface.
Explanation is further explained to the embodiment of the present invention below by embodiment, but does not indicate that and sends out this
Bright protection domain is limited in described in embodiment in scope.
EM saccharomycete in the embodiment of the present invention is purchased to Shanxi Chang Tai animal pharmaceutical estates Co., Ltd, and execution standard is Q/
SCY010-213；Bacillus subtilis is purchased to Wuhan Nature, and product standard card number is Q/WTH05-
2012；
India truffle, summer truffle, Panzhihua ferfas described in embodiment are purchased to Panzhihua Yi ferfas Co., Ltd.
Traditional Indian ferfas described in embodiment, traditional summer truffle, traditional Panzhihua ferfas whether refer to using with
India truffle, summer truffle and Panzhihua ferfas prepared by past method.Specific preparation method is as follows：
Solid ferfas process for preparing strain thereof：First ferfas is crushed and mixed with sterilizing vermiculite or fine earth, ferfas bacterium ball is obtained,
Kept for 7 days under the conditions of 5 DEG C of low temperature.
It is prepared by liquid spawn：Ferfas is crushed according to 1：10 ratio addition water, which is put into mixer, to be stirred and obtains
Kept for 3 days under the conditions of liquid spawn, matching while using, 5 DEG C of low temperature.
Embodiment 1 prepares ferfas inoculation bacterium ball with the inventive method
Ferfas inoculation bacterium ball is prepared, its composition includes：33 parts of ferfas powder, 10 parts of limestone powder, 23 parts of vermiculite power, mud
15 parts of powdered carbon, 2 parts of EM saccharomycete, 5 parts of white sugar, 2 parts of bacillus subtilis.
The preparation method operation of above-mentioned ferfas inoculation bacterium ball is as follows：
Raw material is weighed by above-mentioned composition ,≤0.5mm powder is ground into, stirs and evenly mixs, adopt under the conditions of 1000 turns/min
The bacterium ball of weight 2g/ is pressed into tablet press machine, it is 10% to dry to moisture, and ferfas bacterium ball is made.
The ferfas bacterium ball that embodiment 1 is prepared is used for truffle mycorrhiza fungi seeding cultivating
Pinus yunnanensis, Huashan pine, Chinese chestnut, pecan tree, Quercus acutissima, Qinggang, oriental white oak, hazel are cultivated using seedlings nursing plate and nutritive cube
The aseptic seedlings such as son 3~5 months, after having lateral root to grow, selection thickness, highly consistent nursery stock are inoculated with, and are used during inoculation
Diameter is slightly larger than clean wooden stick or the metal bar punching of bacterium ball diameter, and 5~10cm of hole depth makes a call to 2~3 holes per seedlings, it is inoculated with 2~
3 bacterium balls, are positioned in 20~25 DEG C of seedling raising greenhouse after the completion of inoculation and cultivate, and cultivate 1 month or so, initially form mycorhiza,
After cultivating 6 months, mycorhiza infection rate is high compared with conventional vaccination infection rate more than 85%.Refer to table 1.Truffle mycorrhiza fungi infection rate
=infectious bacteria radical/detection mycorhiza number × 100%.
The bacterium ball Arbuscular Mycorrhizal Fungi of the present invention of table 1 cultivate 6 months after effect
Embodiment 2 prepares ferfas inoculation bacterium ball with the inventive method
Ferfas inoculation bacterium ball is prepared, its composition includes：30 parts of ferfas powder, 8 parts of limestone powder, 20 parts of vermiculite power, mud
10 parts of powdered carbon, EM saccharomycete part, 4 parts of white sugar, 1 part of bacillus subtilis.
The preparation method operation of above-mentioned ferfas inoculation bacterium ball is as follows：
Raw material is weighed by above-mentioned composition ,≤0.5mm powder is ground into, stirs and evenly mixs, adopt under the conditions of 1100 turns/min
The bacterium ball of weight 2g/ is pressed into tablet press machine, it is 10% to dry to moisture, and ferfas bacterium ball is made.
The ferfas bacterium ball that embodiment 2 is prepared is used for ferfas artificial cultivation
In ferfas artificial cultivation garden, along the more position of drop shadow spread's outer fibrous root of tree crown, from 4 different azimuths,
The vertical direction of tree crown is punched, 5~10cm of hole depth.2~4 balls/hole is inoculated with according to the size of tree vigo(u)r, table soil is covered after inoculation
.Observation obtains the result shown in table 2 after being inoculated with 1 time, 4 months.
Application effect in the inoculation bacterium ball ferfas artificial cultivation of the present invention of table 2
Embodiment 3 prepares ferfas inoculation bacterium ball with the inventive method
Ferfas inoculation bacterium ball is prepared, its composition includes：35 parts of ferfas powder, 12 parts of limestone powder, 25 parts of vermiculite power, mud
20 parts of powdered carbon, 2 parts of EM saccharomycete, 6 parts of white sugar, 2 parts of bacillus subtilis.
The preparation method operation of above-mentioned ferfas inoculation bacterium ball is as follows：
Raw material is weighed by above-mentioned composition ,≤0.5mm powder is ground into, stirs and evenly mixs, adopt under the conditions of 1200 turns/min
The bacterium ball of weight 2g/ is pressed into tablet press machine, it is 10% to dry to moisture, and ferfas bacterium ball is made.
The ferfas bacterium ball that embodiment 3 is prepared is inoculated with for the wild ferfas bacterium pool
The relatively low bacterium pool of selection field ferfas yield and the bacterium pool that once production ferfas does not produce now, deep 5~10cm is dug with red rake,
The position inoculation ferfas bacterium ball that fibrous root is flourishing is selected, each ball of vaccination 4~6 is inoculated with once, covering table soil is after the completion of inoculation
Can.Observation obtains the result shown in table 3.
Application effect of the inoculation bacterium ball of the present invention of table 3 in wild ferfas rush is numerous
Can be seen that by embodiment, bacterium ball be inoculated with using the ferfas of the present invention, with higher offspring cultivate infection rate and into
Motility rate, suitably with block mycorrhiza fungi seeding cultivating；In addition, it can increase truffle mycorrhiza fungi quantity, in artificial cultivation, operating efficiency is high,
Mycorhiza quantity is more；The ferfas bacterium ball of the present invention applies also for the ferfas inoculation on the wild mushroom pool, can make not produce the bacterium bacterium pool and start to produce bacterium,
And improve the production bacterium amount on the low yield bacterium pool.
Claims (10)
1. ferfas is inoculated with bacterium ball, it is characterised in that composition includes：Count by weight, 30~35 parts of ferfas powder, agstone
8~12 parts of end, 20~25 parts of vermiculite power, 10~20 parts of peat dust, 1~2 part of EM saccharomycete, 4~6 parts of white sugar, bacillus subtilis
1~2 part of bacterium.
2. ferfas according to claim 1 is inoculated with bacterium ball, it is characterised in that composition includes：Count by weight, ferfas powder
33 parts of end, 10 parts of limestone powder, 23 parts of vermiculite power, 15 parts of peat dust, 2 parts of EM saccharomycete, 5 parts of white sugar, bacillus subtilis 2
Part.
3. ferfas according to claim 1 is inoculated with bacterium ball, it is characterised in that：Described limestone powder, vermiculite power, peat
The particle diameter of powder≤0.5mm.
4. ferfas according to claim 1 is inoculated with bacterium ball, it is characterised in that：Described ferfas be India truffle, summer truffle,
One kind in Panzhihua ferfas, Podbielniak ferfas, black truffle, wealthy spore ferfas or Li Yu ferfas.
5. the ferfas described in any one of Claims 1 to 4 is inoculated with the preparation method of bacterium ball, it is characterised in that comprise the following steps：
A, ferfas strain collection and preservation
Ripe ferfas is chosen, diameter≤0.5mm powder is ground into after sterilizing；
B, preparation ferfas bacterium ball
By 30~35 parts of ferfas powder, 8~12 parts of limestone powder, 20~25 parts of vermiculite power, 10~15 parts of peat dust, EM yeast
1~2 part of bacterium, 4~6 parts of white sugar, 1~2 part of bacillus subtilis weighs above-mentioned raw materials, mixed in 1000~1200 turns/min stirrings
It is even, it is pressed into after the bacterium ball of weight 1.5~2g/ is dried and is packed using tablet press machine, ferfas bacterium ball is made.
6. ferfas according to claim 5 is inoculated with the preparation method of bacterium ball, it is characterised in that：Sterilizing described in step a
Method be：With 70~75% alcohol-pickled 2~3min.
7. ferfas according to claim 5 is inoculated with the preparation method of bacterium ball, it is characterised in that：When being suppressed described in step b
Temperature≤35 DEG C.
8. ferfas according to claim 5 is inoculated with the preparation method of bacterium ball, it is characterised in that：Drying described in step b
To be dried in vacuo or drying in the shade, vacuum drying temperature is 30~35 DEG C, 0.06~0.08MPa of vacuum.
9. ferfas according to claim 5 is inoculated with the preparation method of bacterium ball, it is characterised in that：After being dried described in step b
Bacterium ball water content be 9~12%.
10. the ferfas described in any one of claim 1~9 is inoculated with the application method of bacterium ball, inoculum concentration is 2~10/plant, inoculation
Depth is away from native 5~10cm of surface.
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| CN107980484A (en) * | 2017-11-29 | 2018-05-04 | 云南露源野生真菌研究所（有限合伙） | A kind of method of pinus yunnanensis seed seedling-raising inoculation India truffle |
| CN107996285A (en) * | 2017-11-29 | 2018-05-08 | 云南露源野生真菌研究所（有限合伙） | A kind of method of Huashan pine seed seedling-raising inoculation India truffle |
| CN108934768A (en) * | 2018-07-25 | 2018-12-07 | 四川省农业科学院土壤肥料研究所 | The breeding method of black spore truffle inoculation microbial inoculum and its processing method and mycorhiza |
| CN109258298A (en) * | 2018-09-11 | 2019-01-25 | 云南省农业科学院生物技术与种质资源研究所 | A method of the recovery of the bacterium pool is carried out using ferfas ball seed |
| CN110583359A (en) * | 2019-10-09 | 2019-12-20 | 四川省农业科学院土壤肥料研究所 | Truffle in-situ tending microbial inoculum and preparation and use method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110583359A (en) * | 2019-10-09 | 2019-12-20 | 四川省农业科学院土壤肥料研究所 | Truffle in-situ tending microbial inoculum and preparation and use method thereof |
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| CN107231940B (en) | 2020-05-05 |
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