CN105349435A - Method for cultivating wild truffles - Google Patents
Method for cultivating wild truffles Download PDFInfo
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Abstract
The invention discloses a method for wild truffles. Truffle cultures are collected in the China General Microbiological Culture Collection Center (CGMCC), and the collection number is CGMCC NO.11601. The wild truffles are disinfected and then cut into small blocks with a small knife, the small-block-shaped wild truffles are inoculated into a culture medium to be subjected to slant culturing, and the truffle cultures are obtained; the truffle cultures are inoculated to a culture medium in a nutritional bag; meanwhile, disinfected pine roots are inserted into the nutritional bag to be cultivated, then the whole-bag truffle cultures are divided into small blocks and sowed to a surface layer with pine humid soil, the truffle cultures grow for one year in a natural expanding-propagation mode, the truffles can bear to the pine roots and are picked in November to December serving as the maturest period of the truffles, and the method for planting the wild truffles is achieved. The original fragrance and the original nutrients of the truffles are guaranteed through the truffles obtained with the method. By means of the method, the survival rate of the produced truffles is high and can be 95%.
Description
Technical field
The invention belongs to the method that wild edible fungus culture technology field relates to the cultivation of a kind of wild truffle.
Background technology
Truffle (Truffles) is the general designation of the raw fungi in underground, its member is under the jurisdiction of 3 main groups of fungi: several orders of the Gasteromycete (Gasteromycetes) of the mould order of capsula interna (Endogonales) of Zygomycota (Zygomycota), the Pezizale (Pezizales) of Ascomycota (Ascomycota) and false truffle order (Elaphomycetales) and Basidiomycota (Basidiomycota), also comprise the minority member in imperfect fungi door (Deuteromycota).Wherein, the ferfas in Ascomycota is called as true truffle (truetruffle), and the ferfas of Basidiomycota claims false ferfas (falsetruffle).Truffle herein refers to narrow sense ferfas class, i.e. the kind of Tuber (Tuber).Taxonomy being under the jurisdiction of Ascomycota (Ascomycota), Pezizale (Pezizales), Tuberaceae (Tuberaceae), is the wild edible fungus of a class preciousness.The areas such as Chuxiong Prefecture Yongren County, Lufeng County, two uncles have truffle to distribute.Kind is India truffle (Tuberindicum) mainly, is secondly Tuber Sinense (T.sinense), false depression truffle (T.pseudoexcauatum), has a small amount of Huidong County's truffle (T.huidongense);
The truffle of Chuxiong Yunnan Province mainly occurs in (1) Management of Pinus yunnanensis Pure Forests under 4 kinds of crop types: in main secondary forest in height above sea level 1600-2600 meters, seeds are pinus yunnanensis (pinusyunnanensisFranch.), account for more than 90%, the woods had truffle to occur from 10 years-40 years.(2) oak-pine mixed forest: arbor species mainly Cortex Querci franchetii (QuercusfranchetiiSkan), Yunnan keteleeria fortunei (KeteleeriaeuelynianaMast), Delavay Evergreenchinkapin (CastanopsisdelavayiFranch in standing forest,). yellow hair Qinggang (Cyclobalanopsisdelavayi (Franch.) Schottky), Quercus acutissima (QuercusacutissimaCarr.), pinus yunnanensis (PinusyunnanensisFranch), shrub is water Coriaria sinica (CoriariasinicaMaxim), Fructus Vaccinii Bracteati (VacciniumbracteatumThumb) mainly.Such standing forest mainly based on Fagaceae, or have individual plant pinus yunnanensis to occur, be ferfas occur main standing forest;
(3) oak class broad-leaf forest: standing forest is based on dungarunga and shrub, and shade density are about 60%;
(4) Yunnan keteleeria fortunei woods: in standing forest, arbor species are mainly based on Yunnan keteleeria fortunei (KeteleeriaevelynianaMast), other have a small amount of Cortex Querci franchetii (QuercusfranchetiiSkan), hair leaf Qinggang (Cyclobalanopsiskerrii (Craib) Hu), phyllanthus emblica (PhyllanthusemblicaLinn), wild embankment (Elsholtziaciliata (Thumb.) Hyland).Also be the main standing forest that truffle bacterium occurs;
Truffle be annual growth once, the growth stage of the slow truffle of mycelial growth is to annual April-November, the listing of a small amount of truffle can be had but truffle kernel is white decorative pattern in July, October kernel to be grown to always and become that ancestor is color patterned becomes black decorative pattern to November again, truffle propagation kind is that the truffle by not excavated is planted in propagation, truffle bacterium is long at pine tree root, the MAIN SOILS of truffle is peat soil, with ancestor's look soil, but peat soil is more fragrant, black pine dew is containing rich in protein, there are 18 seed amino acids, comprise 8 kinds of indispensable amino acids that human body can not synthesize, unsaturated fatty acids multivitamin, the essential trace elements such as ferromanganese calcium phosphorus selenium, and sphingolipid, cerebroside, ceramide, the meta-bolites that triterpene androsterone adenosine truffle acid sterol truffle polysaccharide truffle polypeptide etc. are a large amount of, there is high nutritive value,
Wherein androsterone has supporing yang, nurse one's health endocrine remarkable efficacy sphingolipid compound and prevent senile dementia, atherosclerosis and anti-kind of oncocyte toxicity aspect have obvious active polysaccharide polypeptide triterpene to have the effects such as the anti-ageing anti-skin labor of strengthening immunity, can be used for health care;
Black pine is exposed at Europe and is generally used for top and its personal value of food materials is known as cuisines " three large kings " side by side with the senior cuisines such as caviare pate de foie gras.Because its growth conditions is harsh, annual worldwide production is very rare therefore price is high expensive, and Zan, than gold, is called as the Black Diamond on dining table;
The China place of production: China black pine dew place of production mainly concentrates on Yunnan Province with Sichuan Province and circumferentially, and wherein output district comprises the meeting reason in Yunnan Province, Gaoligong Shan Mountain, the ground such as Sichuan (current Panzhihua City is the township of Chinese truffle by benefit).China Dark truffle kind reach tens kinds but actual there is higher edible valency matter be only about 5 kinds, before general black pine is exposed at and does not have fully matured, epidermis is soft and fine and smooth, and square section is creamy white or grey; After maturation, epidermis is hard and have intensive verruca, and square section white marble lines is comparatively obvious.The black pine dew bacterium fragrance that quality is higher is pure, to a kind of comparatively unique nasal receptor of people, according to the examination and analysb of domestic and international biologist, ripe black pine dew can reach the aromatic series natural compounds of more than tens kinds, at present along with the block speed development of Chinese truffle industry, the truffle resource in Yunnan and Sichuan is day by day exhausted, and also when the river rises the boat goes up thereupon for its market business price.Nowadays also do not realize the method for planting, therefore developing a kind of method that wild truffle bacterium bacterial classification is cultivated, is meet the important channel required for people and market.
Summary of the invention
In order to realize the method that truffle is produced, the invention provides a kind of method that wild truffle bacterium bacterial classification is cultivated;
The present invention is realized by following technical proposal: a kind of wild truffle bacterial classification, this bacterial classification at China Committee for Culture Collection of Microorganisms's common micro-organisms center, (CCGMCC) preservation, preserving number is, No. CGMCCNO.11601;
Another object of the present invention is to a kind of method providing wild truffle bacterium bacterial classification to cultivate, through the following step: A, pluck the wild truffle that fresh head is large, after epidermal area pocket knife is scraped clean earth, truffle epidermis is being cleared up with volumetric concentration 75% alcohol, under aseptic tune part, epidermal area pocket knife is pruned, use alcohol clean surface, at the frustillatum that cuts with a knife;
B, is inoculated in following media by the truffle of steps A gained bulk; Semen Maydis powder 1200 ~ 1500 Fen ﹑ analysis for soybean powder 1200 ~ 1500 Fen ﹑ pine tar 1440 ~ 1600 Fen ﹑ glucose powder 1200 ~ 1500 Fen ﹑ vitamin B15 .2 ~ 8 Fen ﹑ yeast 46 ~ 66 Fen ﹑ sodium selenite 1.2 ~ 1.5 Fen ﹑ calcium carbonate 1.2 ~ 1.5 Fen ﹑ magnesium sulfate 4.8 ~ 6.5 Fen ﹑ potassium primary phosphate 4.8 ~ 6.5 Fen ﹑ carries out slant culture 25 ~ 40 days at 23 ~ 26 DEG C, obtain truffle bacterial classification, its preserving number: CGMCCNO, No. 11601.
Truffle length block in described steps A is 1 ~ 1.5cm thickness, 0.2 ~ 0.5cm width, 0.3 ~ 0.6cm.
The slant culture of described step B completes in test tube.
Another object of the present invention is that a kind of method providing wild truffle bacterium bacterial classification to cultivate comprises the following steps:, and (preserving number is that the pinus yunnanensis pine root of disinfecting inserts in this nutrient bag substratum by the wild truffle strain inoculation of No. CGMCCNO11601 in the substratum of nutrient bag simultaneously by 1) ﹑, cultivate 40 ~ 60 days at 22 ~ 25 DEG C, cultivate at pine tree root and obtain s-generation truffle bacterial classification: the substratum of described nutrient bag is made up of the component of following masses: pine 1400 ~ 1600 parts of ﹑ corns 200 ~ 400 parts of ﹑ analysis for soybean powder 200 ~ 400 parts of ﹑ wheat bran 50 ~ 60 parts of ﹑ gypsum 10 ~ 15 parts of ﹑ white sugar 50 ~ 60 parts of ﹑ pine tars 50 ~ 60 parts of ﹑ yeast 25 ~ 50 parts of ﹑ potassium primary phosphates 5 ~ 10 parts of ﹑ magnesium sulfate 5 ~ 10 parts of ﹑,
(2) ﹑ take out step (1) gained s-generation truffle bacterial classification, and s-generation bacterial classification is divided into several parts and has been sown in pine tree and sterile peat top layer, the moisture of peat will keep drenching as well, time of broadcasting sowing carried out in rainy season in summer and autumn, obtain expand numerous after by its self-sow, luminosity is at 500 ~ 650lux, summer and autumn average daily temperature at 17 ~ 26 DEG C, humidity 70 ~ 80%, wind-force 1 ~ 2 grade, until the July in rainy season of Second Year-November truffle just president at pine tree root, plucking kernel 11 ~ December is black decorative pattern, also be the best period of plucking truffle,
(3) use tooth pitch sparse rake when ﹑ plucks, gently dig trying plane, the 11 ~ December of digging the soil opened and bury first-class Second Year can be plucked truffle again after having dug, so achieve the method that Yunnan Wild truffle is cultivated;
The pine root of disinfecting in described step (1) refers to and the pine root of the length 25 ~ 35cm diameter 1 ~ 1.5cm survived is cut into inclined-plane, remove out bifurcated, carry out clearing up and sterilizing with the alcohol that volumetric concentration is 75% again, until adhere to without earth, then tree root is inserted 10cm in nutrient bag, and seal, keep dry behaviour to ventilate;
It is that whole for bacterial classification bag is divided into the peat top layer that 10 ~ 20 little bulks are sown in pine tree that described step (2) is broadcasted sowing, and each pine tree broadcasts sowing 3 ~ 5 bags of truffle bacterial classifications.
The peat of disinfecting in described step (2) covers pine tree soil top layer after the soft water disinfection with 100 DEG C.
Described step (2) time of broadcasting sowing carries out in annual June ~ November.
The pine tree of described step (2) refers to be transplanted in level land or tiltedly pine tree, and soil is from pineland soil, concrete operations are: from pine forests soil movement level land, ground or tiltedly will pave, and be arranged with sequence by pine tree plantation on level land or tiltedly go up, and manage and protect routinely and make pine tree grow more than 1 year, the movement of peat soil soil is put pine tree soil top layer pave between 30 ~ 35cm thick, sterile peat is covered peat soil top layer.
Described step (2) pine tree is pinus yunnanensis pine tree, and the age of tree is 5 ~ 25 years.
Described step (3) soil be height above sea level at 1600 ~ 2600m, pH value is between 5 ~ 6.5, and organic matter is the peat soil in 50% ~ 80% pine forests;
The advantage that the present invention possesses and effect: the present invention is sown in the peat top layer of pine root by bacterial classification, bacterial classification expands numerous by forever there is pine tree root, utilize the self-sow rule of manually building the Nature, the environment of truffle self-sow is being built into by transplanting breeding, with 5 ~ 25 years pinus yunnanensis and peat soil soil for condition, build the sandstone soil that mountain forest is the same, the mode of cultivating with slant strains again can obtain truffle bacterial classification in a large number, can broadcast sowing a bacterial classification again if yielded poorly.The truffle of the inventive method gained is used to make a variation, mouthfeel fragrance and wild truffle are identical, ensure that original fragrance and the nutrition of truffle, the surviving rate of production truffle of the present invention is high, can 95% be reached, and need not agriculture chemical be used, the collecting period plucks once with wild truffle for the same 1 year, achieves the method that wild truffle bacterium bacterial classification is cultivated;
embodiment: below by embodiment, the present invention will be further described.
Embodiment 1
A, pluck the wild truffle that fresh head is large, after epidermal area pocket knife is scraped clean earth, truffle epidermis is being cleared up with volumetric concentration 75% alcohol, under aseptic tune part, epidermal area pocket knife is pruned, using alcohol clean surface, be 1cm thickness be 0.2cm width is 0.3cm in the frustillatum length that cuts with a knife;
B, the truffle of steps A gained bulk is inoculated in following media; Semen Maydis powder 1200 Fen ﹑ analysis for soybean powder 1200 Fen ﹑ pine tar 1440 Fen ﹑ glucose powder 1200 Fen ﹑ vitamin B5 .2 Fen ﹑ yeast 46 Fen ﹑ sodium selenite 1.2 Fen ﹑ calcium carbonate 1.2 Fen ﹑ magnesium sulfate 4.8 Fen ﹑ potassium primary phosphate 4.8 Fen ﹑ carries out slant culture 25 days at 26 DEG C, obtain truffle bacterial classification, its preserving number: CGMCCNO, No. 11601;
Preserving number is that the wild truffle strain inoculation of No. CGMCCNO11601 is in the substratum of nutrient bag by C ﹑, be that the pine root of 1cm is cut into inclined-plane by the long 25cm survived, diameter, remove out bifurcated, carry out clearing up and sterilizing with the alcohol that volumetric concentration is 75% again, until without earth attachment, then tree root is inserted 10cm in nutrient bag, and seal, keep dry behaviour to ventilate, cultivate 40 days at 26 DEG C, cultivate and obtain s-generation truffle bacterial classification; The substratum of described nutrient bag is made up of the component of following masses part: pine 1400 Fen ﹑ corn 200 Fen ﹑ analysis for soybean powder 200 Fen ﹑ wheat bran 50 Fen ﹑ gypsum 10 Fen ﹑ white sugar 50 Fen ﹑ pine tar 50 Fen ﹑ yeast 25 Fen ﹑ potassium primary phosphate 5 Fen ﹑ magnesium sulfate 5 Fen ﹑;
E, by the pine tree of 15 years arrangement in order plantation on level land, and manage and protect routinely and make pine tree grow more than 1 year, by from peat soil pH value 5.5 organic matter 60% of height above sea level 2500m pineland, movement to level land, tile, thickness is 35cm, by peat with the soft water disinfection of 100 DEG C after cover pine tree soil top layer, in rainy season in August then; Step C is expanded numerous obtained s-generation bacterial classification, and whole bag is divided into the peat top layer that 10 little bulks are sown in pine tree; Every pine tree broadcasts sowing 5 bags of bacterial classifications, and keep luminosity 550lux, average daily temperature 17 ~ 26 DEG C, humidity 80%, wind-force 1 grade, until the July in rainy season of Second Year ~ November truffle will tie at pine tree root, and carry out truffle harvesting in 11 ~ December then, be also the most mature period of truffle;
F, when plucking with the sparse rake of tooth pitch, gently dig trying plane, after having dug, the 11 ~ December of digging the soil opened and bury first-class Second Year can plucked again, so achieve the method that Yunnan Wild truffle bacterium bacterial classification is cultivated, the survival rate of truffle of the present invention can reach more than 95%.
Implementation column 2
A, pluck the wild truffle that fresh head is large, after epidermal area pocket knife is scraped clean earth, truffle epidermis is being cleared up with volumetric concentration 75% alcohol, under aseptic tune part, epidermal area pocket knife is pruned, using alcohol clean surface, be 1.2cm thickness be 0.4cm width is 0.5cm in the frustillatum length that cuts with a knife;
B, the truffle of steps A gained bulk is inoculated in following media; Semen Maydis powder 1300 Fen ﹑ analysis for soybean powder 1300 Fen ﹑ pine tar 1500 Fen ﹑ glucose powder 1300 Fen ﹑ VITMAIN B1 6.5 Fen ﹑ yeast 55 Fen ﹑ sodium selenite 1.4 Fen ﹑ calcium carbonate 1.4 Fen ﹑ magnesium sulfate 5.5 Fen ﹑ potassium primary phosphate 5.5 Fen ﹑ carries out slant culture 32 days at 24 DEG C, obtain truffle bacterial classification, its preserving number: CGMCCNO, No. 11601;
Preserving number is that the wild truffle strain inoculation of No. CGMCCNO11601 is in the substratum of nutrient bag by C ﹑, by the long 30cm survived, diameter be 1.2 pine root be cut into inclined-plane, remove out bifurcated, carry out clearing up and sterilizing with the alcohol that volumetric concentration is 75% again, until without earth attachment, then tree root is inserted 10cm in nutrient bag, and seal, keep dry behaviour to ventilate, cultivate 50 days at 25 DEG C, cultivate and obtain s-generation truffle bacterial classification; The substratum of described nutrient bag is made up of the component of following masses part: pine 1500 Fen ﹑ corn 300 Fen ﹑ analysis for soybean powder 300 Fen ﹑ wheat bran 55 Fen ﹑ gypsum 12 Fen ﹑ white sugar 55 Fen ﹑ pine tar 55 Fen ﹑ yeast 30 Fen ﹑ potassium primary phosphate 8 Fen ﹑ magnesium sulfate 8 Fen ﹑;
E, by the pine tree of 10 years arrangement in order plantation on level land, and manage and protect routinely and make pine tree grow more than 1 year, by from peat soil pH value 5.8 organic matter 55% of height above sea level 2200m pineland, movement to level land, tile, thickness is 32cm, by peat with the soft water disinfection of 100 DEG C after cover pine tree soil top layer, in rainy season in September then; Step C is expanded numerous obtained s-generation bacterial classification, and whole bag is divided into the peat top layer that 15 little bulks are sown in pine tree; Every pine tree broadcast sowing 4 bags of bacterial classifications and keep luminosity 600lux, average daily temperature 17 ~ 25 DEG C, humidity 75%, wind-force 1 grade, until the July in rainy season of Second Year ~ November truffle will tie at pine tree root, and carrying out truffle harvesting in 11 ~ December then, is also the most mature period of truffle;
F, when plucking with the sparse rake of tooth pitch, gently dig trying plane, after having dug, the 11 ~ December of digging the soil opened and bury first-class Second Year can plucked again, so achieve the method that Yunnan Wild truffle bacterium bacterial classification is cultivated, the survival rate of truffle of the present invention can reach more than 92%.
Implementation column 3
A, pluck the wild truffle that fresh head is large, after epidermal area pocket knife is scraped clean earth, truffle epidermis is being cleared up with volumetric concentration 75% alcohol, under aseptic tune part, epidermal area pocket knife is pruned, using alcohol clean surface, be 1.5cm thickness be 0.5cm width is 0.6cm in the frustillatum length that cuts with a knife;
B, the truffle of steps A gained bulk is inoculated in following media; Semen Maydis powder 1500 Fen ﹑ analysis for soybean powder 1500 Fen ﹑ pine tar 1600 Fen ﹑ glucose powder 1500 Fen ﹑ VITMAIN B1 8 Fen ﹑ yeast 66 Fen ﹑ sodium selenite 1.5 Fen ﹑ calcium carbonate 1.5 Fen ﹑ magnesium sulfate 6.5 Fen ﹑ potassium primary phosphate 6.5 Fen ﹑ carries out slant culture 40 days at 23 DEG C, obtain truffle bacterial classification, its preserving number: CGMCCNO, No. 11601;
Preserving number is that the wild truffle strain inoculation of No. CGMCCNO11601 is in the substratum of nutrient bag by C ﹑, by the long 35cm survived, diameter be 1.5 pine root be cut into inclined-plane, remove out bifurcated, carry out clearing up and sterilizing with the alcohol that volumetric concentration is 75% again, until without earth attachment, then tree root is inserted 10cm in nutrient bag, and seal, keep dry behaviour to ventilate, cultivate 60 days at 23 DEG C, cultivate and obtain s-generation truffle bacterial classification; The substratum of described nutrient bag is made up of the component of following masses part: pine 1600 Fen ﹑ corn 400 Fen ﹑ analysis for soybean powder 400 Fen ﹑ wheat bran 60 Fen ﹑ gypsum 15 Fen ﹑ white sugar 60 Fen ﹑ pine tar 60 Fen ﹑ yeast 50 Fen ﹑ potassium primary phosphate 10 Fen ﹑ magnesium sulfate 10 Fen ﹑;
E, by the pine tree of 8 years arrangement in order plantation on level land, and manage and protect routinely and make pine tree grow more than 1 year, by from peat soil pH value 6 organic matter 50% of height above sea level 1800m pineland, movement to level land, tile, thickness is 30cm, by peat with the soft water disinfection of 100 DEG C after cover pine tree soil top layer, in rainy season in October then; Step C is expanded numerous obtained s-generation bacterial classification, and whole bag is divided into the peat top layer that 20 little bulks are sown in pine tree; Every pine tree broadcast sowing 3 bags of bacterial classifications and keep luminosity 650lux, average daily temperature 17 ~ 24 DEG C, humidity 70%, wind-force 2 grades, until the July in rainy season of Second Year ~ November truffle will tie at pine tree root, and carry out truffle harvesting in 11 ~ December then, be the most mature period of truffle;
With the rake that tooth pitch is sparse when F, harvesting, gently digging trying plane, after having dug, burying 11 ~ 12 of first-class Second Year digging the soil opened
The moon can be plucked again, and so achieve the method that Yunnan Wild truffle bacterium bacterial classification is cultivated, the survival rate of truffle of the present invention can reach more than 90%.
Claims (10)
1. the method that wild truffle kind is cultivated is characterized in that: this bacterial classification is in Chinese microorganism strain preservation management microorganism center (CGMCC) preservation, and preserving number is: CGMCCNO11601.
2. a method for wild truffle kind cultivation, is characterized in that through following each step:
A, plucks the wild truffle that fresh head is large, after epidermal area pocket knife is scraped clean earth, with volumetric concentration 75% alcohol cleaning truffle epidermis, under aseptic tune part, epidermal area pocket knife is pruned, uses alcohol clean surface, at the frustillatum that cuts with a knife
B, is inoculated in following media by the truffle of steps A gained bulk; Semen Maydis powder 1200 ~ 1500 Fen ﹑ analysis for soybean powder 1200 ~ 1500 Fen ﹑ pine tar 1440 ~ 1600 Fen ﹑ glucose powder 1200 ~ 1500 Fen ﹑ vitamin B15 .2 ~ 8 Fen ﹑ yeast 46 ~ 66 Fen ﹑ sodium selenite 1.2 ~ 1.5 Fen ﹑ calcium carbonate 1.2 ~ 1.5 Fen ﹑ magnesium sulfate 4.8 ~ 6.5 Fen ﹑ potassium primary phosphate 4.8 ~ 6.5 Fen ﹑ carries out slant culture 25 ~ 40 days at 23 ~ 26 DEG C, obtain truffle bacterial classification, its preserving number: CGMCCNO, No. 11601.
3. preparation method according to claim 2, is characterized in that: block truffle bacterial classification in described steps A,
Length 1 ~ 1.5cm, thickness 0.2 ~ 0.5cm, width 0.3 ~ 0.6cm.
4. preparation method according to claim 2, is characterized in that: the slant culture of described step B completes in test tube.
5. truffle bacterium bacterial classification cultivates a method for wild truffle, it is characterized in that comprising the following steps:
(preserving number is that the wild truffle strain inoculation of No. CGMCCNO11601 is to the substratum of nutrient bag by 1) ﹑
Middlely the pinus yunnanensis pine root of disinfecting is inserted in this nutrient bag substratum simultaneously, cultivate 40 ~ 60 days at 22 ~ 25 DEG C, cultivate at pine tree root and obtain s-generation truffle bacterial classification: the substratum of described nutrient bag is made up of the component of following masses: pine 1400 ~ 1600 parts of ﹑ corns 200 ~ 400 parts of ﹑ analysis for soybean powder 200 ~ 400 parts of ﹑ wheat bran 50 ~ 60 parts of ﹑ gypsum 10 ~ 15 parts of ﹑ white sugar 50-60 parts of ﹑ pine tars 50 ~ 60 parts of ﹑ yeast 25 ~ 50 parts of ﹑ potassium primary phosphates 5 ~ 10 parts of ﹑ magnesium sulfate 5 ~ 10 parts of ﹑;
(2) ﹑ take out step (1) gained s-generation truffle bacterial classification, and s-generation bacterial classification is divided into several parts and has been sown in pine tree and sterile peat top layer, the moisture of peat will keep drenching as well, time of broadcasting sowing carried out in rainy season in summer and autumn, obtain expand numerous after by its self-sow, luminosity is at 500 ~ 650lux, summer and autumn average daily temperature at 17 ~ 26 DEG C, humidity 70 ~ 80%, wind-force 1 ~ 2 grade, until the July in rainy season of Second Year ~ November truffle just president at pine tree root, plucking kernel 11 ~ December is black decorative pattern, also be the best period of plucking truffle,
(3) use tooth pitch sparse rake when ﹑ plucks, gently dig trying plane, the 11 ~ December of digging the soil opened and bury first-class Second Year can be plucked truffle again after having dug, so achieve the method for Yunnan Wild truffle plantation.
6. method according to claim 5, it is characterized in that: the tree root of disinfecting in (1) is that the pine root of the length 25 ~ 35cm diameter 1 ~ 1.5cm survived is cut into inclined-plane, remove out bifurcated, carry out clearing up and sterilizing with the alcohol that volumetric concentration is 75% again, until adhere to without earth, then tree root is inserted 10cm in nutrient bag, and seal, keep dry behaviour to ventilate.
7. method according to claim 5, it is characterized in that: described step (2) s-generation truffle bacterial classification is divided into several parts and refers to: the s-generation truffle bacterial classification covered with in whole bag nutrient bag is divided into 10 ~ 20 little bulks and broadcasts sowing 3 ~ 5 bags, broadcast sowing in 6 ~ November then.
8. method according to claim 5, is characterized in that: the peat in described step (2) after sterilization is the soft water disinfection with 100 DEG C.
9. method according to claim 5, it is characterized in that: the pine tree of described step (2) refers to be transplanted in level land or tiltedly pine tree, and soil is from pineland soil, concrete operations are: from pine forests soil movement level land, ground or tiltedly will pave, and be arranged with sequence by pine tree plantation on level land or tiltedly go up, and manage and protect routinely and make pine tree grow more than 1 year, the movement of peat soil soil is put pine tree soil top layer pave between 30 ~ 35cm thick, sterile peat is covered peat soil top layer.
10. method according to claim 9, is characterized in that: described pine tree is the pinus yunnanensis age of tree is 5 ~ 25 years; Described soil is height above sea level is more than 5 ~ 6.5 in 1600 ~ 2600mPH matter, and organic matter is the peat soil soil of 50 ~ 80%.
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| CN105917913A (en) * | 2016-04-28 | 2016-09-07 | 四川省农业科学院土壤肥料研究所 | Method for breeding mycorrhizal seedlings by truffles and quercus acutissima |
| CN105998095A (en) * | 2016-06-29 | 2016-10-12 | 宫晶晶 | Preparation method and specific culture medium of truffle oral solution |
| CN106978354A (en) * | 2017-03-11 | 2017-07-25 | 云南绿辰生物科技开发有限公司 | A kind of breeding method of high-purity ferfas strain |
| CN108157097A (en) * | 2017-12-27 | 2018-06-15 | 贵州省生物研究所 | A kind of Radix Codonopsis Pregermination and seedling breeding method |
| CN110896780A (en) * | 2019-11-05 | 2020-03-24 | 香格里拉市山里情菌业有限责任公司 | Artificial propagation-promoting industrial conservation method for tricholoma matsutake |
| TWI714499B (en) * | 2020-04-21 | 2020-12-21 | 耕詮股份有限公司 | Method for inoculating economically valuable truffles to Castanea plants to form mycorrhizal seedlings |
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| CN105917913A (en) * | 2016-04-28 | 2016-09-07 | 四川省农业科学院土壤肥料研究所 | Method for breeding mycorrhizal seedlings by truffles and quercus acutissima |
| CN105998095A (en) * | 2016-06-29 | 2016-10-12 | 宫晶晶 | Preparation method and specific culture medium of truffle oral solution |
| CN106978354A (en) * | 2017-03-11 | 2017-07-25 | 云南绿辰生物科技开发有限公司 | A kind of breeding method of high-purity ferfas strain |
| CN106978354B (en) * | 2017-03-11 | 2020-06-16 | 云南绿辰生物科技开发有限公司 | Cultivation method of high-purity truffle strain |
| CN108157097A (en) * | 2017-12-27 | 2018-06-15 | 贵州省生物研究所 | A kind of Radix Codonopsis Pregermination and seedling breeding method |
| CN110896780A (en) * | 2019-11-05 | 2020-03-24 | 香格里拉市山里情菌业有限责任公司 | Artificial propagation-promoting industrial conservation method for tricholoma matsutake |
| TWI714499B (en) * | 2020-04-21 | 2020-12-21 | 耕詮股份有限公司 | Method for inoculating economically valuable truffles to Castanea plants to form mycorrhizal seedlings |
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