TWI714499B - Method for inoculating economically valuable truffles to Castanea plants to form mycorrhizal seedlings - Google Patents

Abstract

The method for inoculating economically valuable truffles to plants of the genus Castanea to form mycorrhizal seedlings belongs to the field of agricultural seedling cultivation, and relates to a method for inoculating three kinds of truffle Tuber elevatireticulatum, Tuber lijiangnese, and Tuber indicum on the roots of plants of the genus Castanea to cultivate mycorrhizal seedlings. The chance of successfully combining the roots of the saplings with the truffle hyphae is greatly improved. The steps are as follows: (1) Cultivation of Castanea seedlings; (2) Castanea seedlings inoculated with truffle hyphae; (3) Castanea plant seedling management. The key to the present invention is to use Castanea plants as hosts for truffle fungi. Castanea plant saplings are inoculated with truffle fungi to form mycorrhizal, which is beneficial to the development of their roots, improves the survival rate and growth and metabolism rate of Castanea plant saplings, and significantly improves root activity , Is conducive to the survival of plants. The invention greatly improves the success rate of seedling inoculation with specified truffle hyphae.

Description

Method for inoculating economically valuable truffles to Castanea plants to form mycorrhizal seedlings

The invention relates to a method for inoculating three kinds of truffles, Tuber elevatireticulatum, Tuber lijiangnese, and Tuber indicum, to seedlings of Castanea plants to form mycorrhizal seedlings.

The genus Tuber, also known as the genus Truffle, is a genus of the Ascomycota phylum Truffle family; the genus Tuber belongs to mycorrhizal fungi and often produces mycorrhiza with other plants. Truffle is rich in more than ten kinds of amino acids and contains essential trace elements that cannot be synthesized by the human body. It has a unique and strong fragrance. It is used as a precious seasoning in high-end restaurant dishes. It is listed in Europe as one of the world's top three delicacies and is a noble ingredient. Due to the slow growth of truffles, the growth conditions and environmental requirements have a certain degree of difficulty, which leads to low yields and requires artificial cultivation to meet market demand. Therefore, effective inoculation methods are the most important success or failure key to truffle production.

Plants of the genus Castanea grow up to more than ten meters high, and are distributed from sea level to 2000 meters above sea level. They are native to various provinces in mainland China and introduced and cultivated in the middle-altitude mountainous area from Taiwan. After maturation, the kernels contain high amounts of protein, fat, carbohydrates, vitamin B and fatty swellings, etc. They are mostly used as roasted food for humans or as food sources for forest animals. The wood plots are hard and heavy, and are a good source of wood for fine furniture and structures.

At present, there is no domestic technology for inoculating truffle seedlings of Castanea plants. The applicant in this case transferred technology from the Forestry Laboratory of the Agricultural Committee of the Executive Yuan. After obtaining truffle strains, he is committed to the complete truffle production industry. Choosing Castanea plants can not only harvest The underground truffle truffles and the mature fruit produced on the trees of the genus Castanea are also another agricultural product with high economic value and harvestable.

＜The technical problem to be solved＞ In view of the fact that there is currently no domestic cultivation technology for inoculating truffle seedlings in Castanea, the inventor hopes to provide a breakthrough technology to complete the truffle production industry and improve the practical effects after obtaining truffle strains. Based on his many years of relevant professional technical knowledge and practical experience, and the results of research and design, he finally developed an economically valuable method for inoculating truffles to Castanea plants to form mycorrhizal seedlings to improve seedling inoculation. Specify the success rate of truffle mycelium.

＜Technical means to solve the problem＞ The invention aims at improving the inoculation tree species in the artificial cultivation of truffles. Choosing Castanea plants as the tree species for planting truffles can not only harvest truffle truffles, but also increase the maturation results of the Castanea plants as harvested products, which greatly improves economic benefits.

The present invention proposes a method for raising seedlings of Castanea plants to inoculate three truffles, Tuber elevatireticulatum, Tuber lijiangnese, Tuber indicum, and the specific steps are as follows:

Step (1) Cultivating seedlings of Castanea plants: Get freshly harvested Castanea seeds, sterilize them in bleach water for 10 minutes, take them out, rinse them with sterile water, soak them in sterile water for 3-5 days, then take them out and wait for germination. After germination, the seeds are taken out and planted in a round soft pot with a diameter of 10 cm and a height of 10 cm with the culture soil.

Step (2) Castanea plant seedlings are inoculated with truffle hyphae: Tuber elevatireticulatum, Tuber lijiangnese, Tuber indicum truffle strains, the aforementioned truffle strains are obtained through technology transfer from the Forestry Laboratory of the Council of Agriculture of the Executive Yuan. Cultivate the seedlings of the Castanea plant in the previous step for 2 months, and implant the truffle mycelium that has been properly inoculated into the castanea seedling pot.

Step (3) Castanea plant seedling management: Put potted seedlings of Castanea plants at room temperature 20-25℃, water once a week, wait for about 2-4 months, then take out the potted plants and observe whether the roots are inoculated with bacteria Silk is successful. If the inoculation is successful, the formation of mycorrhizal can be observed in the growth of the roots. When the mycorrhizal formation is about 80%, it can be moved to the field for planting.

The present invention adopts Castanea plant seedlings cultivated in culture soil, which has high mycorrhizal compatibility. Inoculated with specified truffle mycelium can make the truffle hypha inoculation rate reach more than 90%; 3-5 after transplantation of the inoculated tree seedlings It enters the harvest period of Castanea plants in the year, and enters the harvest period of truffle truffles after 5-7 years, which can form a complete industry and ecology.

<Comparing the effects of previous technologies> The main purpose of the present invention is to use Castanea plants as hosts for truffle fungi. Castanea plant saplings are inoculated with truffle fungi to form mycorrhiza, which is beneficial to the development of their roots, and improves the survival rate and growth and metabolism rate of Castanea plant saplings. The activity is significantly improved, which is conducive to the survival of plants.

The second purpose of the present invention is to use the method of the present invention to greatly increase the success rate of seedlings inoculation with specified truffle hyphae.

In order to enable your reviewer to better understand the structural features and effects of the present invention, the figures are combined with detailed descriptions below.

Please refer to the first figure. The present invention aims to improve the inoculation tree species in the artificial cultivation of truffles. Choosing Castanea plants as the tree species for planting truffles can not only harvest truffle truffles, but also increase the maturation results of Castanea plants as a harvest. The product greatly improves economic efficiency.

The present invention proposes a method for raising seedlings of Castanea plants to inoculate three truffles, Tuber elevatireticulatum, Tuber lijiangnese, Tuber indicum, and the specific steps are as follows:

Step (1) Cultivating seedlings of Castanea plants: Obtain freshly harvested Castanea plant seeds, sterilize them in bleach water for 10 minutes, take them out and rinse them with sterile water, and then rinse and soak them in sterile water for 3-5 days. Take out the seeds of Castanea plants and wait for germination. After germination, take out the seeds of Castanea plants to cultivate soil and plant them in a round soft pot with a diameter of 10 cm and a height of 10 cm. The culture soil substrate used for growing seedlings is controlled at pH 7- 7.5.

Step (2) Castanea plant seedlings are inoculated with truffle hyphae: Tuber elevatireticulatum, Tuber lijiangnese, Tuber indicum truffle strains, which are obtained through technology transfer from the Forestry Laboratory of the Council of Agriculture of the Executive Yuan. Cultivate the seedlings of the Castanea plant in the previous step for 2 months, and implant the truffle mycelium that has been properly inoculated into the castanea seedling pot.

Step (3) Castanea plant seedling management: inoculate potted seedlings of Castanea plants at room temperature 20-25℃, humidity 70-90%, illumination 6000-10000 lux, water at least once a week, and wait After about 2-4 months, take out the potted plants and observe whether the roots are successfully inoculated with hyphae. If the inoculation is successful, the formation of mycorrhizal can be observed in the growth of the roots. When the mycorrhizal formation is about 80%, it can be moved to the field for planting.

The said Castanea plants grow in an area with an altitude of about 100-2000 meters.

The present invention adopts Castanea plant seedlings cultivated in culture soil, which has high mycorrhizal compatibility. Inoculated with specified truffle mycelium can make the truffle hypha inoculation rate reach more than 90%; 3-5 after transplantation of the inoculated tree seedlings It enters the harvest period of Castanea plants in the year, and enters the harvest period of truffle truffles after 5-7 years, which can form a complete industry and ecology.

In summary, the present invention improves the survival rate and growth metabolism rate of Castanea plant saplings, and the root activity is significantly improved, which is conducive to the survival of the plant. At the same time, it greatly improves the success rate of inoculating saplings with specified truffle hyphae. Inventions that are novel, progressive, and available for industrial application, have actually met the requirements for granting patents for inventions. I filed a patent application in accordance with the law. I hope that the examiner can examine it in detail and grant the patent for this case as soon as possible. Poop.

Only the above are only the preferred embodiments of the present invention. When they cannot be used to limit the scope of the present invention, all equivalent changes and modifications made in accordance with the scope of the patent application of the present invention shall still belong to the patent of the present invention. Covered.

The first figure is a block flow diagram of the method of the present invention.

Claims (6)

The method for inoculating truffles into plants of the genus Castanea to form mycorrhizal seedlings relates to a method of inoculating three truffles Tuber elevatireticulatum, Tuber lijiangnese, and Tuber indicum into the roots of plants of the genus Castanea to cultivate mycorrhizal seedlings, wherein truffles and plants of the genus Castanea cultivate mycorrhizal seedlings, The steps include: (1) cultivating Castanea plant seedlings, (2) Castanea plant seedlings inoculation with truffle hyphae, and (3) Castanea plant seedling management; the step (1) cultivating Castanea plant seedlings: obtain fresh harvest Castanea seeds should be sterilized in bleaching water for 10 minutes. After removal, rinse with sterile water and soak in sterile water for 3-5 days. Then take out Castanea seeds and wait for germination. After germination, take out Castanea seeds for cultivation The soil is planted in a round soft pot with a diameter of 10 cm and a height of 10 cm; the step (2) Castanea plant seedlings are inoculated with truffle hyphae: Tuber elevatireticulatum, Tuber lijiangnese, Tuber indicum truffle strains, and castanea from the previous step Plant seedlings of the genus are cultivated for 2 months, and the mycelium that has been properly inoculated is implanted into the seedlings of the genus Castanea; the step (3) the management of the seedlings of the genus Castanea: put the potted seedlings of the genus Castanea that have been inoculated in the previous step In the greenhouse room temperature environment, water at least once a week, wait for about 2-4 months and then take out the potted plant to observe whether the root inoculation is successful. If the inoculation is successful, the formation of mycorrhiza can be observed in the growth of the root. It can be planted in the field at 80%. The method for inoculating truffles to plants of the genus Castanea to form mycorrhizal seedlings as described in claim 1, wherein the plants of the genus Castanea are grown in an area of 100-2000 meters above sea level. The method for inoculating truffles to Castanea plants to form mycorrhizal seedlings as described in claim 1, wherein the seedling cultivation soil substrate is controlled at pH 7-7.5. The method for inoculating truffles into Castanea plants to form mycorrhizal seedlings as described in claim 1, wherein the temperature in the seedling growing greenhouse is 20-25°C. The method for inoculating truffles to Castanea plants to form mycorrhizal seedlings as described in claim 1, wherein the humidity in the seedling growing greenhouse is 70-90%. The method for inoculating truffles into Castanea plants to form mycorrhizal seedlings as described in claim 1, wherein the illuminance in the seedling growing greenhouse is 6000-10000 lux.

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