CN107333566A - A kind of method that russule is cultivated using Asiatic plantain Va Mycorrhiza Seedling - Google Patents
A kind of method that russule is cultivated using Asiatic plantain Va Mycorrhiza Seedling Download PDFInfo
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- CN107333566A CN107333566A CN201710709500.6A CN201710709500A CN107333566A CN 107333566 A CN107333566 A CN 107333566A CN 201710709500 A CN201710709500 A CN 201710709500A CN 107333566 A CN107333566 A CN 107333566A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention discloses a kind of method that russule is cultivated using Asiatic plantain Va Mycorrhiza Seedling, comprise the following steps：A. Isolation and Culture；B. strain expands numerous；C. Asiatic plantain mycorrhiza fungi seeding cultivating and cultivation fruiting.Contain Chinese herbaceous peony grass polysaccharide in the mother culture media used in the Isolation and Culture.Compared with prior art, beneficial effects of the present invention are：Cultivation russule is aided in using Asiatic plantain Va Mycorrhiza Seedling, artificial cultivation is realized, is laid the foundation for the industrialization production of russule；In addition, addition Chinese herbaceous peony grass polysaccharide can improve the amino acid content in russule obtained by cultivation in mother culture media.
Description
Technical field
The invention belongs to edible fungus technical field of cultivation, and in particular to a kind of to cultivate russule using Asiatic plantain Va Mycorrhiza Seedling
Method.
Background technology
Russule (Russula), is under the jurisdiction of Basidiomycetes, russule mesh, Russulaceae, Russula, and main product has very high in thick woods
Edible medicinal value rare edible mycorrhizal fungi.317 kinds of Russula fungies have been reported in the world, and China records about more than 90 and planted.
Abroad, russule is distributed mainly on the U.S., Japan, Korea, Russia；At home, Fujian, Liaoning, Jiangsu, cloud are mainly distributed on
The provinces and regions such as south, Anhui, Hebei, Guangxi.Russule delicious flavour, containing abundant nutriment, protein and polyoses content are high, amino
Sour A wide selection of colours and designs, also contain volatile materials, ergosterol and Substance, be always that dinner party is entertained guests, welcome gives a present
It is rare to serve and present good merchantable brand, it is in great demand at home and abroad, the market demand is more vigorous, and natural resources is reduced year by year again, but without scale
The artificial cultivation of change supports the current market demand.
The content of the invention
It is more vigorous for russule demand in the prior art, and the present situation that natural resources is deficient, it is an object of the invention to
A kind of method that russule is cultivated using Asiatic plantain Va Mycorrhiza Seedling is provided, it is full to be laid the foundation for the artificial large-scale planting of russule
The active demand of sufficient Vehicles Collected from Market.
For achieving the above object, the technical solution adopted by the present invention is as follows：
A kind of method that russule is cultivated using Asiatic plantain Va Mycorrhiza Seedling, is comprised the following steps：
A. Isolation and Culture：New fresh sporophore is gathered, cuts bacterial context band part tube tissue block, accesses Mother culture
Base, culture；
B. strain expands numerous：Gained parent species access will be cultivated and be equipped with quiescent culture in the container for expanding breeding culture medium, treat parent species block
Start a large amount of russule bacterium balls occur in concussion and cultivate to container after sprouting；
C. Asiatic plantain mycorrhiza fungi seeding cultivating and cultivation fruiting：The bacterium ball for expanding numerous acquisition is broken into bacterium slurry, by sterile Chinese herbaceous peony
Careless seedling is cultivated again after dipping in bacterium slurry, Asiatic plantain Va Mycorrhiza Seedling is cultivated into, then by Asiatic plantain Va Mycorrhiza Seedling dense planting sterile-processed
Nursery lot, cultivate fruiting.
Further, described every liter of water of mother culture media includes the composition of following parts by weight：Glucose 12-20 parts,
(NH4)2SO42.2-4.6 part, ZnSO44.6-6 part, VB10.08-0.18 parts, potato juice 20-30 parts, agar 20-26 parts, Chinese herbaceous peony
Grass polysaccharide 0.8-4 parts.
Further, described every liter of water of mother culture media includes the composition of following parts by weight：Glucose 14-18 parts,
(NH4)2SO42.6-4 part, ZnSO45-5.7 part, VB10.1-0.16 parts, potato juice 22-28 parts, agar 22-24 parts, Asiatic plantain
Polysaccharide 1-3 parts.
Further, described every liter of water of mother culture media includes the composition of following parts by weight：16 parts of glucose,
(NH4)2SO43.4 parts, ZnSO45.3 parts, VB10.13 part, 25 parts of potato juice, 23 parts of agar, 2.4 parts of Chinese herbaceous peony grass polysaccharide.
Further, the extracting method of the Chinese herbaceous peony grass polysaccharide is：Dry Chinese herbaceous peony grass meal is taken, with 1g：15-20ml material
Liquor ratio adds 2-3 rear suction filtration of 75-80 DEG C of distilled water immersion 1.2-1.5h, 400-440W Microwave Extraction, merges extract solution, adds
Chloroform：N-butanol (5:1) fully shaken up after mixed liquor, centrifuging and taking supernatant, add ethanol precipitation polysaccharide, after washing drying be
Can.
Further, the cultivation temperature during the cultivation fruiting is 25-27 DEG C, humidity 65-85%.
Compared with prior art, beneficial effects of the present invention are：Cultivation russule is aided in using Asiatic plantain Va Mycorrhiza Seedling, realizes people
Work is cultivated, and is laid the foundation for the industrialization production of russule；In addition, Chinese herbaceous peony grass polysaccharide is added in mother culture media can improve cultivation
Amino acid content in gained russule.
Embodiment
To make those skilled in the art be better understood when technical scheme, with reference to embodiment pair
Technical solution of the present invention is described in detail.
Experimental method used in following embodiments is conventional method unless otherwise specified.
Material used, reagent etc., unless otherwise specified, are commercially obtained in following embodiments.
Embodiment 1
A kind of method that russule is cultivated using Asiatic plantain Va Mycorrhiza Seedling, is comprised the following steps：
A. Isolation and Culture：New fresh sporophore is gathered, cuts bacterial context band part tube tissue block, accesses Mother culture
Base, culture；
B. strain expands numerous：Gained parent species access will be cultivated and be equipped with quiescent culture in the container for expanding breeding culture medium, treat parent species block
Start a large amount of russule bacterium balls occur in concussion and cultivate to container after sprouting；
C. Asiatic plantain mycorrhiza fungi seeding cultivating and cultivation fruiting：The bacterium ball for expanding numerous acquisition is broken into bacterium slurry, by sterile Chinese herbaceous peony
Careless seedling is cultivated again after dipping in bacterium slurry, Asiatic plantain Va Mycorrhiza Seedling is cultivated into, then by Asiatic plantain Va Mycorrhiza Seedling dense planting sterile-processed
Nursery lot, cultivate fruiting.
The composition of following parts by weight can wherein be included in every liter of water of mother culture media：12 parts of glucose, (NH4)2SO42.2 parts, ZnSO44.6 parts, VB10.08 part, 20 parts of potato juice, 20 parts of agar, 0.8 part of Chinese herbaceous peony grass polysaccharide
Embodiment 2
A kind of method that russule is cultivated using Asiatic plantain Va Mycorrhiza Seedling, is comprised the following steps：
A. Isolation and Culture：New fresh sporophore is gathered, cuts bacterial context band part tube tissue block, accesses Mother culture
Base, culture；
B. strain expands numerous：Gained parent species access will be cultivated and be equipped with quiescent culture in the container for expanding breeding culture medium, treat parent species block
Start a large amount of russule bacterium balls occur in concussion and cultivate to container after sprouting；
C. Asiatic plantain mycorrhiza fungi seeding cultivating and cultivation fruiting：The bacterium ball for expanding numerous acquisition is broken into bacterium slurry, by sterile Chinese herbaceous peony
Careless seedling is cultivated again after dipping in bacterium slurry, Asiatic plantain Va Mycorrhiza Seedling is cultivated into, then by Asiatic plantain Va Mycorrhiza Seedling dense planting sterile-processed
Nursery lot, cultivate fruiting.
The composition of following parts by weight can wherein be included in every liter of water of mother culture media：14 parts of glucose, (NH4)2SO42.6 parts, ZnSO45 parts, VB10.1 part, 22 parts of potato juice, 22 parts of agar, 1 part of Chinese herbaceous peony grass polysaccharide.
Embodiment 3
A kind of method that russule is cultivated using Asiatic plantain Va Mycorrhiza Seedling, is comprised the following steps：
A. Isolation and Culture：New fresh sporophore is gathered, cuts bacterial context band part tube tissue block, accesses Mother culture
Base, culture；
B. strain expands numerous：Gained parent species access will be cultivated and be equipped with quiescent culture in the container for expanding breeding culture medium, treat parent species block
Start a large amount of russule bacterium balls occur in concussion and cultivate to container after sprouting；
C. Asiatic plantain mycorrhiza fungi seeding cultivating and cultivation fruiting：The bacterium ball for expanding numerous acquisition is broken into bacterium slurry, by sterile Chinese herbaceous peony
Careless seedling is cultivated again after dipping in bacterium slurry, Asiatic plantain Va Mycorrhiza Seedling is cultivated into, then by Asiatic plantain Va Mycorrhiza Seedling dense planting sterile-processed
Nursery lot, cultivate fruiting.
The composition of following parts by weight can wherein be included in every liter of water of mother culture media：16 parts of glucose, (NH4)2SO43.4 parts, ZnSO45.3 parts, VB10.13 part, 25 parts of potato juice, 23 parts of agar, 2.4 parts of Chinese herbaceous peony grass polysaccharide.
Embodiment 4
A kind of method that russule is cultivated using Asiatic plantain Va Mycorrhiza Seedling, is comprised the following steps：
A. Isolation and Culture：New fresh sporophore is gathered, cuts bacterial context band part tube tissue block, accesses Mother culture
Base, culture；
B. strain expands numerous：Gained parent species access will be cultivated and be equipped with quiescent culture in the container for expanding breeding culture medium, treat parent species block
Start a large amount of russule bacterium balls occur in concussion and cultivate to container after sprouting；
C. Asiatic plantain mycorrhiza fungi seeding cultivating and cultivation fruiting：The bacterium ball for expanding numerous acquisition is broken into bacterium slurry, by sterile Chinese herbaceous peony
Careless seedling is cultivated again after dipping in bacterium slurry, Asiatic plantain Va Mycorrhiza Seedling is cultivated into, then by Asiatic plantain Va Mycorrhiza Seedling dense planting sterile-processed
Nursery lot, cultivate fruiting.
The composition of following parts by weight can wherein be included in every liter of water of mother culture media：18 parts of glucose, (NH4)2SO44
Part, ZnSO45.7 parts, VB10.16 part, 28 parts of potato juice, 24 parts of agar, 3 parts of Chinese herbaceous peony grass polysaccharide.
Embodiment 5
A kind of method that russule is cultivated using Asiatic plantain Va Mycorrhiza Seedling, is comprised the following steps：
A. Isolation and Culture：New fresh sporophore is gathered, cuts bacterial context band part tube tissue block, accesses Mother culture
Base, culture；
B. strain expands numerous：Gained parent species access will be cultivated and be equipped with quiescent culture in the container for expanding breeding culture medium, treat parent species block
Start a large amount of russule bacterium balls occur in concussion and cultivate to container after sprouting；
C. Asiatic plantain mycorrhiza fungi seeding cultivating and cultivation fruiting：The bacterium ball for expanding numerous acquisition is broken into bacterium slurry, by sterile Chinese herbaceous peony
Careless seedling is cultivated again after dipping in bacterium slurry, Asiatic plantain Va Mycorrhiza Seedling is cultivated into, then by Asiatic plantain Va Mycorrhiza Seedling dense planting sterile-processed
Nursery lot, cultivate fruiting.
The composition of following parts by weight can wherein be included in every liter of water of mother culture media：20 parts of glucose, (NH4)2SO44.6 parts, ZnSO46 parts, VB10.18 part, 30 parts of potato juice, 26 parts of agar, 4 parts of Chinese herbaceous peony grass polysaccharide.
Chinese herbaceous peony grass polysaccharide in embodiment 1-5 mother culture medias can be prepared by the following method gained：Take dry car
Preceding grass meal, with 1g：15-20ml solid-liquid ratio adds 75-80 DEG C of distilled water immersion 1.2-1.5h, 400-440W Microwave Extraction 2-3
Filtered after secondary, merge extract solution, add chloroform：N-butanol (5:1) fully shaken up after mixed liquor, centrifuging and taking supernatant, add second
Alcohol precipitate polysaccharides, dried after washing.
Embodiment 6
A kind of method that russule is cultivated using Asiatic plantain Va Mycorrhiza Seedling, is comprised the following steps：
A. Isolation and Culture：New fresh sporophore is gathered, cuts bacterial context band part tube tissue block, accesses Mother culture
Base, culture；
B. strain expands numerous：Gained parent species access will be cultivated and be equipped with quiescent culture in the container for expanding breeding culture medium, treat parent species block
Start a large amount of russule bacterium balls occur in concussion and cultivate to container after sprouting；
C. Asiatic plantain mycorrhiza fungi seeding cultivating and cultivation fruiting：The bacterium ball for expanding numerous acquisition is broken into bacterium slurry, by sterile Chinese herbaceous peony
Careless seedling is cultivated again after dipping in bacterium slurry, Asiatic plantain Va Mycorrhiza Seedling is cultivated into, then by Asiatic plantain Va Mycorrhiza Seedling dense planting sterile-processed
Nursery lot, cultivate fruiting.
Wherein, the cultivation temperature during cultivating fruiting can be 25-27 DEG C, and humidity can be 65-85%.
Comparative example 1
Except Chinese herbaceous peony grass polysaccharide is not added in mother culture media, remaining condition is the same as embodiment 3.
The content of amino acid in embodiment 3 and the cultivation gained russule of comparative example 1 is determined using conventional amino acid assay method,
It the results are shown in Table 1.
The embodiment 3 of table 1 and the content of amino acid in the cultivation gained russule of comparative example 1
| Russule source | Polyoses content/% |
| Embodiment 3 | 25.16 |
| Comparative example 1 | 18.82 |
As known from Table 1, the amino acid that adding Chinese herbaceous peony grass polysaccharide in mother culture media can improve in russule obtained by cultivation contains
Amount.
Obviously, above-described embodiment is only the section Example of the present invention, rather than whole embodiments.Based on opening for the present invention
Show, those of ordinary skill in the art's all other embodiment resulting on the premise of creative work is not made, all
Belong to the scope that the present invention is protected.
Claims (6)
- A kind of 1. method that russule is cultivated using Asiatic plantain Va Mycorrhiza Seedling, it is characterised in that comprise the following steps：A. Isolation and Culture：New fresh sporophore is gathered, cuts bacterial context band part tube tissue block, accesses mother culture media, training Support；B. strain expands numerous：Gained parent species access will be cultivated and be equipped with quiescent culture in the container for expanding breeding culture medium, treat that parent species block is sprouted After start occur a large amount of russule bacterium balls in concussion and cultivate to container；C. Asiatic plantain mycorrhiza fungi seeding cultivating and cultivation fruiting：The bacterium ball for expanding numerous acquisition is broken into bacterium slurry, by sterile Asiatic plantain seedling Cultivated again after dipping in bacterium slurry, Asiatic plantain Va Mycorrhiza Seedling is cultivated into, then by the dense planting of Asiatic plantain Va Mycorrhiza Seedling in sterile-processed nursery Ground, cultivate fruiting.
- 2. method according to claim 1, it is characterised in that described every liter of water of mother culture media includes following parts by weight Composition：Glucose 12-20 parts, (NH4)2SO42.2-4.6 part, ZnSO44.6-6 part, VB10.08-0.18 parts, potato juice 20-30 parts, agar 20-26 parts, Chinese herbaceous peony grass polysaccharide 0.8-4 parts.
- 3. method according to claim 2, it is characterised in that described every liter of water of mother culture media includes following parts by weight Composition：Glucose 14-18 parts, (NH4)2SO42.6-4 part, ZnSO45-5.7 part, VB10.1-0.16 parts, potato juice 22- 28 parts, agar 22-24 parts, Chinese herbaceous peony grass polysaccharide 1-3 parts.
- 4. method according to claim 3, it is characterised in that described every liter of water of mother culture media includes following parts by weight Composition：16 parts of glucose, (NH4)2SO43.4 parts, ZnSO45.3 parts, VB10.13 part, 25 parts of potato juice, 23 parts of agar, 2.4 parts of Chinese herbaceous peony grass polysaccharide.
- 5. according to any one of claim 2-4 methods described, it is characterised in that the extracting method of the Chinese herbaceous peony grass polysaccharide is：Take Dry Chinese herbaceous peony grass meal, with 1g：15-20ml solid-liquid ratio adds 75-80 DEG C of distilled water immersion 1.2-1.5h, 400-440W microwave 2-3 rear suction filtration of extraction, merges extract solution, adds chloroform：N-butanol (5:1) fully shaken up after mixed liquor, centrifuging and taking supernatant, Ethanol precipitation polysaccharide is added, is dried after washing.
- 6. method according to claim 1, it is characterised in that the cultivation temperature during the cultivation fruiting is 25-27 DEG C, wet Spend for 65-85%.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110278828A (en) * | 2019-07-10 | 2019-09-27 | 新疆农业科学院植物保护研究所 | A kind of method of Pleurotus nebrodensis field child care on the spot |
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| CN105474992A (en) * | 2015-11-28 | 2016-04-13 | 楚雄彝族自治州林业科学研究所 | Chinese chestnut summer truffle mycorrhizal seedling culture method based on symbiotic relation |
| CN106171533A (en) * | 2016-09-05 | 2016-12-07 | 广西大学 | A kind of cultural method of Russula |
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2017
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| CN101204131A (en) * | 2007-12-10 | 2008-06-25 | 丽水市林业科学研究所 | Artificial cultivation method for Suillus Luteus |
| CN104186201A (en) * | 2014-08-26 | 2014-12-10 | 景洪宏臻农业科技有限公司 | Earth balls artificial cultivation method |
| CN105474992A (en) * | 2015-11-28 | 2016-04-13 | 楚雄彝族自治州林业科学研究所 | Chinese chestnut summer truffle mycorrhizal seedling culture method based on symbiotic relation |
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| CN110278828A (en) * | 2019-07-10 | 2019-09-27 | 新疆农业科学院植物保护研究所 | A kind of method of Pleurotus nebrodensis field child care on the spot |
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