CN110278828A - A kind of method of Pleurotus nebrodensis field child care on the spot - Google Patents
A kind of method of Pleurotus nebrodensis field child care on the spot Download PDFInfo
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- CN110278828A CN110278828A CN201910619014.4A CN201910619014A CN110278828A CN 110278828 A CN110278828 A CN 110278828A CN 201910619014 A CN201910619014 A CN 201910619014A CN 110278828 A CN110278828 A CN 110278828A
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- pleurotus nebrodensis
- asafoetide
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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Abstract
The invention discloses a kind of methods of Pleurotus nebrodensis field child care on the spot, by preparing Pleurotus nebrodensis sporophore homogenate, by Pleurotus nebrodensis sporophore homogenate field inoculation in asafoetide plant, establish the technology of Pleurotus nebrodensis sporophore homogenate field inoculation asafoetide plant, averagely successfully field planting rate is 83.02% to Pleurotus ferulae mycelium, realize the field planting of Pleurotus ferulae mycelium, and this method is simple and efficient, large-scale asafoetide plant inoculating is carried out suitable for field, Pleurotus ferulae mycelium be can effectively improve in the field planting efficiency of wild asafoetide plant root, it makes important progress in the technology of child care on the spot of Pleurotus nebrodensis germ plasm resource, Pleurotus nebrodensis germ plasm resource is set to protect and develop, there is wide applicability in terms of the protection of Pleurotus nebrodensis germ plasm resource.
Description
Technical field
The present invention relates to edible mushroom germ plasm resource field child care technical fields, specifically, the present invention relates to a kind of Pleurotus nebrodensis
The method and technology field of field child care on the spot.
Background technique
Pleurotus nebrodensis, Chinese rectification of name Pleurotus nebrodensis (Pleurotus tuoliensis), be it is a kind of food, medical value it is all very high
Rare delicious edible mushroom." the The famous cultivated mushroom that MengranZhao in 2016 et al. is delivered
Bailinggu is a separate species of thePleurotus eryngii species complex " article
It wore in " fungus journal " to kindly help secure the success of et al. with 2018 and deliver in " Chinese five kinds of important edible mushroom scientific names are newly infused " article, pass through and be
Both of Pleurotus ferulae Lanzi and Pleurotus nebrodensis fungies are distinguished in system development research, independent for 2 in systematic growth
Branch, and supporting rate is very high, and in addition, 2 kinds of distributed areas are also different, therefore Pleurotus nebrodensis is promoted to one independent kind.
In China, it is domestic that wild Pleurotus nebrodensis is only distributed in Xinjiang, is distributed mainly on the Resina Ferulae beach of Zhungeer Basin Edge gobi, and select
Property foundation portion tissue that is saprophytic or colonizing in Chinese medicine asafoetide plant, it is annual only in the first tenday period of a month fruiting in late April to May, distribution
It measures very rare.In recent years, since mankind's activity increases, the deterioration of the ecological environment, climate change, and resource is excessively excavated,
Wild Pleurotus nebrodensis resource ownership sharply declines, and the wild Pleurotus nebrodensis in some regions almost disappears.Therefore, Pleurotus nebrodensis resource is just
Ground child care and protection technique are urgently to be resolved.Currently, the research about Pleurotus nebrodensis germ plasm resource field child care technology has not been reported,
And Pleurotus nebrodensis strain fructification homogenate field inoculation asafoetide plant technology is established, realize Pleurotus ferulae mycelium in asafoetide plant root
The field planting of portion's tissue and fruiting, are of great significance to the child care on the spot and its ecological protection of Pleurotus nebrodensis wild resource.
Research team of the present invention discloses a kind of asafoetide on December 26th, 2016, the patent CN106613351A declared
The method of mushroom liquid strain field inoculation asafoetide plant discloses the preparation of asafoetide mushroom liquid strain and Pleurotus ferulae liquid in the invention
The method of strain field inoculation asafoetide plant, the efficiency that this method prepares liquid spawn is lower, higher cost, while out of office external
In kind asafoetide plant process, due to Gobi desert drought, day temperature is higher, causes partially liq strain to inactivate, serious shadow
Child care efficiency and mycelial field planting success rate and stability are rung, therefore this team draws on the experience, with regard to the child care of Pleurotus nebrodensis field
Technical method is advanced optimized and is innovated.
Summary of the invention
For in the prior art, asafoetide mushroom liquid strain preparation efficiency is low, and asafoetide mushroom liquid strain field inoculation asafoetide is planted
The liquid spawn of the method preparation of object the problems such as liquid spawn easy in inactivation, has no about white in field inoculation asafoetide plant process
Clever mushroom germ plasm resource field child care superior technique status on the spot, the present invention is intended to provide a kind of child care on the spot of Pleurotus nebrodensis field
Method, by Pleurotus nebrodensis sporophore homogenate field inoculation in asafoetide plant, establishes Pleurotus nebrodensis by preparing Pleurotus nebrodensis sporophore homogenate
Fructification is homogenized the technology of field inoculation asafoetide plant, and realizes the field planting of Pleurotus ferulae mycelium, and this method is simple and efficient, fits
Large-scale asafoetide plant inoculating is carried out for field, prior art objective reality is overcome the shortcomings of, can effectively improve Bai Ling
Mycelium obtains important in the field planting efficiency of wild asafoetide plant root in the technology of child care on the spot of Pleurotus nebrodensis germ plasm resource
Progress, enables Pleurotus nebrodensis germ plasm resource to protect and develop, and has extensive be applicable in terms of the protection of Pleurotus nebrodensis germ plasm resource
Property.
In order to achieve the above object, the present invention is to be achieved through the following technical solutions.
The present invention specifically provides a kind of method of Pleurotus nebrodensis field child care on the spot, comprising the following steps:
(1) Pleurotus nebrodensis sporophore selects, and selects the Pleurotus nebrodensis sporophore of artificial cultivation, to guarantee that fructification sprouts into bacterium again
The vigor of filament, fructification answer it is fresh, without mildew, disease-free insect infection, be preferred with the Pleurotus nebrodensis sporophore newly picked；
(2) Pleurotus nebrodensis sporophore prepared by step (1) is cut into fungus block, is put into tissue by Pleurotus nebrodensis sporophore homogenate preparation
In crusher, the sterile water of same volume is added, fructification is broken into homogenate, is poured into container, 1/3~1/2 volume is added
Sterile water dilution, obtain Pleurotus nebrodensis sporophore homogenate, for use；
(3) asafoetide Plant choosing observes asafoetide plant and its rhizome portion, selects non-bolting, and 2-3 is raw, and root is not white
The asafoetide plant of clever mycelium field planting；
(4) asafoetide plant root is handled, and the tools such as asafoetide foundation portion's soil spades of step (3) selection are carefully dug
It opens, depth is advisable with 17cm~20cm, and diameter is advisable with 17cm~25cm, is exposed asafoetide plant roots base portion, not destroyed asafoetide
The humus in foundation portion；
(5) Pleurotus nebrodensis sporophore homogenate inoculation asafoetide plant, the Pleurotus nebrodensis sporophore for taking 130-150ml step (2) to prepare
Homogenate, is uniformly poured onto asafoetide plant roots base portion root tissue, infiltrates into soil slightly to moisture, will initially dig the soil opened
It all is backfilled to asafoetide foundation portion, is gently compacted earthing；
(6) moisturizing of asafoetide plant root is handled, and humidity is an important factor for guaranteeing mycelium sprouting and vigor, in order to improve
Mycelium is colonized effect, need to carry out moisturizing processing to the inoculation position of asafoetide plant, layer of plastic film is covered on asafoetide root
On the earthing of base portion, film is flattened, surrounding is fixed with bamboo stick, is then covered with one layer of soil again and pushes down film, can both prevent too
Positive direct projection causes asafoetide plant roots base portion high temperature to kill mycelium, and film can be prevented to be blown away.
Preferably, the easy fermentation of Pleurotus nebrodensis sporophore homogenate is rotten in the present invention, to guarantee that fructification homogenate is sprouted into again
The Pleurotus nebrodensis sporophore homogenate that step (2) prepares is stored in the space for being protected from light, divulging information, cooling, temperature by mycelial vigor
Degree is advisable, the holding time was no more than 12 hours no more than 25 DEG C with 4 DEG C~20 DEG C.
Preferably, Pleurotus nebrodensis sporophore is cut into the fungus block of 3cm~6cm diameter in the present invention, is put into historrhexis
In machine, the sterile water of same volume is added, fructification is broken into homogenate, is poured into container, the sterile water for adding 1/2 volume is dilute
It releases, obtains Pleurotus nebrodensis sporophore homogenate.
Preferably, it takes 140ml Pleurotus nebrodensis sporophore to be homogenized in the present invention, is uniformly poured onto asafoetide plant roots base portion root tissue
On.
Preferably, non-bolting, life in the 2nd year, the asafoetide plant that root does not have Pleurotus ferulae mycelium to be colonized are selected in the present invention.
By using the technical solution of above-mentioned offer, the present invention obtain it is following the utility model has the advantages that
(1) method of a kind of Pleurotus nebrodensis field provided by the invention child care on the spot, by preparing Pleurotus nebrodensis sporophore homogenate,
By Pleurotus nebrodensis sporophore homogenate field inoculation in asafoetide plant, the skill of Pleurotus nebrodensis sporophore homogenate field inoculation asafoetide plant is established
Art, averagely successfully field planting rate is 83.02% to Pleurotus ferulae mycelium, realizes the field planting of Pleurotus ferulae mycelium, and this method is easy to be fast
Victory is suitable for field and carries out large-scale asafoetide plant inoculating, can effectively improve Pleurotus ferulae mycelium in wild asafoetide plant
The field planting efficiency of root makes important progress in the technology of child care on the spot of Pleurotus nebrodensis wild resource, makes Pleurotus nebrodensis wild resource
It is able to protect and develop, there is wide applicability in terms of the protection of Pleurotus nebrodensis wild resource.
(2) method of a kind of Pleurotus nebrodensis field provided by the invention child care on the spot, humidity are to guarantee that mycelium is sprouted and living
An important factor for power, after Pleurotus nebrodensis sporophore homogenate is inoculated in asafoetide plant, by plastic covering film to asafoetide plant
Inoculation position carries out moisturizing processing, and mycelium is made to be able to maintain the vigor of long period, is conducive to the child care of Pleurotus nebrodensis field.
Detailed description of the invention
Attached drawing 1 show asafoetide plant root moisturizing processing figure.
Attached drawing 2 show Pleurotus ferulae mycelium in asafoetide plant root colonisation figure, wherein the white area that arrow refers to
For the Pleurotus ferulae mycelium for being colonized in asafoetide plant roots stem.
Specific embodiment
In order to which technical problems, technical solutions and advantages to be solved are more clearly understood, tie below
Embodiment is closed, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only used to solve
The present invention is released, is not intended to limit the present invention.Below with reference to the embodiment technical solution that the present invention will be described in detail, but protection scope
It is not limited by this.
Embodiment one:
(1) Pleurotus nebrodensis sporophore selects, and selects the Pleurotus nebrodensis sporophore of artificial cultivation, to guarantee that fructification sprouts into bacterium again
The vigor of filament, fructification answer it is fresh, without mildew, disease-free insect infection, be preferred with the Pleurotus nebrodensis sporophore newly picked；
(2) Pleurotus nebrodensis sporophore prepared by step (1) is cut into fungus block, is put into tissue by Pleurotus nebrodensis sporophore homogenate preparation
In crusher, the sterile water of same volume is added, fructification is broken into homogenate, is poured into container, 1/3~1/2 volume is added
Sterile water dilution, obtain Pleurotus nebrodensis sporophore homogenate, for use；
(3) asafoetide Plant choosing observes asafoetide plant and its rhizome portion, selects non-bolting, 2-3 is raw, and root does not have
The asafoetide plant of Pleurotus ferulae mycelium field planting；
(4) asafoetide plant root is handled, and the tools such as asafoetide foundation portion's soil spades of step (3) selection are carefully dug
It opens, depth is advisable with 17cm~20cm, and diameter is advisable with 17cm~25cm, is exposed asafoetide plant roots base portion, not destroyed asafoetide
The humus in foundation portion；
(5) Pleurotus nebrodensis sporophore homogenate inoculation asafoetide plant, the Pleurotus nebrodensis sporophore for taking 130-150ml step (2) to prepare
Homogenate, is uniformly poured onto asafoetide plant roots base portion root tissue, infiltrates into soil slightly to moisture, will initially dig the soil opened
It all is backfilled to asafoetide foundation portion, is gently compacted earthing.
(6) moisturizing of asafoetide plant root is handled, and humidity is an important factor for guaranteeing mycelium sprouting and vigor, in order to improve
Mycelium is colonized effect, need to carry out moisturizing processing to the inoculation position of asafoetide plant, layer of plastic film is covered on asafoetide root
On the earthing of base portion, film is flattened, surrounding is fixed with bamboo stick, is then covered with one layer of soil again and pushes down film, can both prevent too
Positive direct projection causes asafoetide plant roots base portion high temperature to kill mycelium, and film can be prevented to be blown away, referring to figure 1.
Preferably, the easy fermentation of Pleurotus nebrodensis sporophore homogenate is rotten in the present invention, to guarantee that fructification homogenate is sprouted into again
Mycelial vigor, the fructification homogenate prepared should be stored in the space be protected from light, divulge information, cooling, and temperature is no more than 25
DEG C, it is advisable with 4 DEG C~20 DEG C, the holding time was no more than 12 hours.
Preferably, Pleurotus nebrodensis sporophore is cut into the fungus block of 3cm~6cm diameter in the present invention, is put into historrhexis
In machine, the sterile water of same volume is added, fructification is broken into homogenate, is poured into container, the sterile water for adding 1/2 volume is dilute
It releases, obtains Pleurotus nebrodensis sporophore homogenate.
Embodiment two:
(1) Pleurotus nebrodensis sporophore selects, and selects the Pleurotus nebrodensis sporophore of artificial cultivation, to guarantee that fructification sprouts into bacterium again
The vigor of filament, fructification answer it is fresh, without mildew, disease-free insect infection, be preferred with the Pleurotus nebrodensis sporophore newly picked；
(2) Pleurotus nebrodensis sporophore prepared by step (1) is cut into fungus block, is put into tissue by Pleurotus nebrodensis sporophore homogenate preparation
In crusher, the sterile water of same volume is added, fructification is broken into homogenate, is poured into container, the sterile of 1/2 volume is added
Water dilution obtains Pleurotus nebrodensis sporophore homogenate, for use；
(3) asafoetide Plant choosing, observation asafoetide plant and its rhizome portion, the non-bolting of selection, life in the 2nd year, root be not white
The asafoetide plant of clever mycelium field planting；
(4) asafoetide plant root is handled, and the tools such as asafoetide foundation portion's soil spades of step (3) selection are carefully dug
It opens, depth is advisable with 17cm~20cm, and diameter is advisable with 17cm~25cm, is exposed asafoetide plant roots base portion, not destroyed asafoetide
The humus in foundation portion；
(5) Pleurotus nebrodensis sporophore homogenate inoculation asafoetide plant, the Pleurotus nebrodensis sporophore homogenate for taking 140ml step (2) to prepare,
It is uniformly poured onto asafoetide plant roots base portion root tissue, is infiltrated into soil slightly to moisture, will initially dig the soil whole opened
It is backfilled to asafoetide foundation portion, is gently compacted earthing.
(6) moisturizing of asafoetide plant root is handled, and humidity is an important factor for guaranteeing mycelium sprouting and vigor, in order to improve
Mycelium is colonized effect, need to carry out moisturizing processing to the inoculation position of asafoetide plant, layer of plastic film is covered on asafoetide root
On the earthing of base portion, film is flattened, surrounding is fixed with bamboo stick, is then covered with one layer of soil again and pushes down film, can both prevent too
Positive direct projection causes asafoetide plant roots base portion high temperature to kill mycelium, and film can be prevented to be blown away.
Preferably, the easy fermentation of Pleurotus nebrodensis sporophore homogenate is rotten in the present invention, to guarantee that fructification homogenate is sprouted into again
Mycelial vigor, the fructification homogenate prepared should be stored in the space be protected from light, divulge information, cooling, and temperature is no more than 25
DEG C, it is advisable with 4 DEG C~20 DEG C, the holding time was no more than 12 hours.
Embodiment three:
It probes into four factors for influencing Pleurotus nebrodensis field planting rate (Y): being diluted with water (A), Pleurotus nebrodensis after Pleurotus nebrodensis homogenate in fact
Body homogenate inoculation asafoetide phytomass (B), asafoetide plant growth time (C), carry out the experiment of four factors, three horizontal quadrature, orthogonal test
Factor and water-glass are shown in Table 1.
Table 1: orthogonal test factor and water-glass
Orthogonal optimization Pleurotus nebrodensis field planting rate test result and analysis are shown in Table 2.
Table 2: orthogonal experiments and analysis
From table 2 it can be seen that being diluted with water after Pleurotus nebrodensis homogenate to Pleurotus nebrodensis field planting rate in the range of experimental design
It influences maximum.Obtaining optimum optimizing condition in conjunction with Tables 1 and 2 is A1B2C2, i.e., add the dilution of 1/2 water, Pleurotus nebrodensis after Pleurotus nebrodensis homogenate
Fructification homogenate inoculation 140g is 13-24 months in asafoetide plant root, asafoetide plant growth time, Pleurotus nebrodensis field planting rate highest
It is 84.6%.
The method that Pleurotus nebrodensis sporophore is homogenized field inoculation asafoetide plant is obtained by the above optimum experimental: selection Planting
The Pleurotus nebrodensis sporophore of training, to guarantee that fructification sprouts into mycelial vigor again, fructification answer it is fresh, without mildew, without disease pest
Infection, is preferred with the Pleurotus nebrodensis sporophore newly picked；Pleurotus nebrodensis sporophore is cut into fungus block, is put into historrhexis's machine, and addition is same
Fructification is broken into homogenate, poured into container by the sterile water of volume, is added the sterile water dilution of 1/2 volume, is obtained white spirit
Massee fruiting bodies homogenate selects non-bolting, life in the 2nd year, the asafoetide plant that root does not have Pleurotus ferulae mycelium to be colonized；With works such as spades
The careful plane of tool is opened, and depth is advisable with 17cm~20cm, and diameter is advisable with 17cm~25cm, exposes asafoetide plant roots base portion, should not
Destroy the humus in asafoetide foundation portion；It takes 140ml Pleurotus nebrodensis sporophore to be homogenized, is uniformly poured onto asafoetide plant roots base portion root tissue
On, it is infiltrated into soil slightly to moisture, will initially dig the soil opened and be all backfilled to asafoetide foundation portion, be gently compacted earthing,
Layer of plastic film is covered on the earthing in asafoetide foundation portion, flattens film, surrounding is fixed with bamboo stick, is then covered with one layer again
Soil pushes down film, can not only prevent direct sunlight from asafoetide plant roots base portion high temperature being caused to kill mycelium, but also can prevent thin
Film is blown away；The test for the Pleurotus nebrodensis sporophore homogenate field inoculation asafoetide plant that annual spring, autumn carry out first 1 year is adjusted
It looks into.
Embodiment three:
This programme designs 2 groups of experiments, and A group carries out Pleurotus nebrodensis sporophore by the scheme of embodiment one and is homogenized field inoculation asafoetide
Vegetal tests, B group are inoculated with asafoetide vegetal tests by method in the patent CN106613351A declared is disclosed on December 26th, 2016,
Selection is inoculated in April in spring to May and autumn September part to October.
The test of current year inoculation is in autumn current year September part to October and the April of second year to May to Pleurotus nebrodensis
The test of entity homogenate field inoculation asafoetide plant is investigated, main sprouting, field planting and the growth for investigating Pleurotus ferulae mycelium
Situation, investigation method are as follows: the soil plane of asafoetide foundation portion side is opened with tool, cleans out and exposes asafoetide plant roots base portion group
It knits, is careful not to injure asafoetide foundation portion tissue, observation and the sprouting of record asafoetide foundation portion tissue Pleurotus ferulae mycelium are determined
Plant and growing state.
Table 3: Pleurotus nebrodensis field inoculation asafoetide vegetal tests
Ginseng is shown in Table 3, current year inoculation pilot survey as the result is shown: A group was successfully determined in Pleurotus ferulae mycelium in 2015
Plant rate is 82.86%, 2016 successfully field planting rate be 79.51%, 2017 successfully field planting rate be 86.70%, it is average successfully fixed
Plant rate is 83.02%, and Pleurotus ferulae mycelium colonisation is referring to shown in attached drawing 2, and B group Pleurotus ferulae mycelium in 2015 is successfully determined
Plant rate is 64.28%, 2016 successfully field planting rate be 66.10%, 2017 successfully field planting rate be 60.59%, it is average successfully fixed
Plant rate is 63.66%, and scheme provided by the present application is apparently higher than B in the field planting rate of the wild inoculation asafoetide plant of Pleurotus nebrodensis sporophore
The well-known technique of group.Test result shows that the method is simple and quick using Pleurotus nebrodensis sporophore homogenate field inoculation asafoetide plant,
And Pleurotus ferulae mycelium is able to achieve in the field planting of asafoetide plant roots base portion tissue, it is suitable for field and carries out large-scale asafoetide plant
Inoculation, can effectively improve Pleurotus ferulae mycelium in the field planting efficiency of wild asafoetide plant root.
As described above, the present invention can be realized preferably, the above embodiments are only to preferred implementation side of the invention
Formula is described, and is not intended to limit the scope of the present invention, and without departing from the spirit of the design of the present invention, this field is general
The various changes and improvement that logical technical staff makes technical solution of the present invention, should all fall into present invention determine that protection scope
It is interior.
Claims (7)
1. a kind of method of Pleurotus nebrodensis field child care on the spot, which comprises the following steps:
(1) Pleurotus nebrodensis sporophore selects, and selects the Pleurotus nebrodensis sporophore of artificial cultivation, fructification answer it is fresh, without mildew, without disease pest
Infection, is preferred with the Pleurotus nebrodensis sporophore newly picked；
(2) Pleurotus nebrodensis sporophore prepared by step (1) is cut into fungus block, is put into historrhexis by Pleurotus nebrodensis sporophore homogenate preparation
In machine, the sterile water of same volume is added, fructification is broken into homogenate, is poured into container, sterile water dilution is added, obtains white
Clever massee fruiting bodies homogenate, for use；
(3) asafoetide Plant choosing observes asafoetide plant and its rhizome portion, selects non-bolting, 2-3 is raw, and root does not have white spirit
The asafoetide plant of mycelium field planting；
(4) asafoetide plant root is handled, and the tools such as the asafoetide foundation portion soil spades of step (3) selection are carefully dug and are opened, deep
Degree is advisable with 17cm~20cm, and diameter is advisable with 17cm~25cm, is exposed asafoetide plant roots base portion, not destroyed asafoetide foundation portion
Humus；
(5) Pleurotus nebrodensis sporophore homogenate inoculation asafoetide plant, the Pleurotus nebrodensis sporophore homogenate for taking 130-150ml step (2) to prepare,
It is uniformly poured onto asafoetide plant roots base portion root tissue, is infiltrated into soil slightly to moisture, will initially dig the soil whole opened
It is backfilled to asafoetide foundation portion, is gently compacted earthing；
(6) moisturizing of asafoetide plant root is handled, and layer of plastic film is covered on the earthing in asafoetide foundation portion, and film is flattened,
Surrounding is fixed with bamboo stick, is then covered with one layer of soil again and is pushed down film.
2. a kind of method of Pleurotus nebrodensis field according to claim 1 child care on the spot, which is characterized in that white by what is prepared
Clever massee fruiting bodies homogenate is stored in the space be protected from light, divulge information, cooling, and temperature is advisable no more than 25 DEG C with 4 DEG C~20 DEG C,
Holding time was no more than 12 hours.
3. a kind of method of Pleurotus nebrodensis field according to claim 1 child care on the spot, which is characterized in that Pleurotus nebrodensis is real
Body is cut into the fungus block of 3cm~6cm diameter, is put into historrhexis's machine, adds the sterile water of same volume, fructification is smashed
At homogenate.
4. a kind of method of Pleurotus nebrodensis field according to claim 1 child care on the spot, which is characterized in that take the white spirit of 140ml
Massee fruiting bodies homogenate, is uniformly poured onto asafoetide plant roots base portion root tissue.
5. a kind of method of Pleurotus nebrodensis field according to claim 1 child care on the spot, which is characterized in that the non-bolting of selection,
Life in 2nd year, the asafoetide plant that root does not have Pleurotus ferulae mycelium to be colonized.
6. a kind of method of Pleurotus nebrodensis field according to claim 1 child care on the spot, which is characterized in that smash fructification
It at homogenate, pours into container, adds the sterile water dilution of 1/3~1/2 volume, obtain Pleurotus nebrodensis sporophore homogenate.
7. a kind of method of Pleurotus nebrodensis field according to claim 6 child care on the spot, which is characterized in that smash fructification
It at homogenate, pours into container, adds the sterile water dilution of 1/2 volume, obtain Pleurotus nebrodensis sporophore homogenate.
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Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2009022218A (en) * | 2007-07-20 | 2009-02-05 | Kinokkusu:Kk | Method for culturing new fruit body |
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