CN106613351A - Method for field inoculation of Ferula asafetida plant with pleuratus ferulae liquid strain - Google Patents
Method for field inoculation of Ferula asafetida plant with pleuratus ferulae liquid strain Download PDFInfo
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention provides a method for field inoculation of a Ferula asafetida plant with a pleuratus ferulae liquid strain. The method includes the steps of pleuratus ferulae mycelium preculture, preparation of seeds special for liquid, liquid strain preparation, liquid strain dilution and Ferula asafetida plant selection, Ferula asafetida plant root treatment and pleuratus ferulae liquid strain inoculation, and process inspection verification methods of all the links. Through preparation of the pleuratus ferulae liquid strain and field inoculation of the Ferula asafetida plant with the pleuratus ferulae liquid strain, pleurotus wild fungus mycelia of Ferula asafetida plant rhizomes on a gobi desert can be effectively restored, and great practical significance is achieved for protecting natural wild pleuratus ferulae resources and restoring a gobi desert ecological system.
Description
Technical field
The invention mainly relates to a kind of fungus growing technique field, specifically, the present invention relates to a kind of edible mushroom is wild
Outer child care technical field.
Background technology
Pleurotus ferulae（Pleurotus eryngii var. ferulae）It is that Xinjiang is local to being grown in Carrot family asafoetide
The general designation of the Pleurotus wild mushroom of platymiscium rhizome portion, is all very high Rare edible fungus of a kind of food, medical value.In China,
Its wild resource is distributed only on the Gobi desert Resina Ferulae beach of Junggar Basin, Xinjiang, china edge awful weather, and selective saprophytic or post
The raw rhizome portion in Chinese medicine asafoetide, abundance is rare.Asafoetide plant annual April turns green, and May reaches the growth most productive period, is also asafoetide
The peak period that mushroom occurs, June asafoetide plant starts to dry up, into rest period, until Second Year April turns green again.In recent years, by
In global climate transition, ecological disruption, and people the factors such as the excessive collection and unauthorized and excessive mining of wild resource are made Ah
Wei's mushroom wild resource recoverable amount drastically declines, and the Pleurotus ferulae resource in some regions almost disappears, it would be highly desirable to child care and protection.
The content of the invention
For state of the art, the technical problem of particularly current wild Pleurotus ferulae resource worsening shortages, the application is led to
Cross in the Pleurotus ferulae field labor child care technical research that for years year carries out of Qinghe County Pleurotus ferulae wild resource child care area, set up
The technology of asafoetide mushroom liquid strain field inoculation asafoetide plant, realizes the field planting of Pleurotus ferulae mycelia, there is provided a kind of Pleurotus ferulae
The method of liquid spawn field inoculation asafoetide plant so that wild Pleurotus ferulae resource is able to protect and develops, and has maintained Gobi desert
The fragile ecological environment in beach, has wide applicability in terms of the protection of wild Pleurotus ferulae resource.
To achieve these goals, the technical scheme that the present invention is provided is as follows：
The invention provides a kind of method of asafoetide mushroom liquid strain field inoculation asafoetide plant, is made up of following steps：
Step 1：Preculture pleuratus ferulae comatus mycelium and to Detection；
Step 2：The good mycelium of preculture is seeded on the sawdust medium of sterilization treatment, 22 DEG C～26 DEG C light cultures are treated
Mycelia is covered with as liquid specific kind, and to it quality testing is carried out;Described detection method is：Aseptically, liquid is taken
Special seed is seeded to PDA culture medium flat board, and 22 DEG C～26 DEG C light culture 2d～5d see whether polluted bacteria or fungi, chooses
Liquid specific kind without pollution prepares mycelium suspended liquid and carries out quality testing；
Step 3：Mycelium suspended liquid is diluted into 3 times～5 times with clear water, stand-by liquid spawn is after mixing；
Step 4：Asafoetide mushroom liquid strain field inoculation asafoetide plant：
The asafoetide plant without the field planting of Pleurotus ferulae mycelia is selected, asafoetide foundation portion soil spades plane is opened, depth 5cm～
15cm, asafoetide foundation portion is exposed in the case where the rotten root in asafoetide foundation portion is not destroyed, and takes 80ml～120ml Pleurotus ferulae liquid bacterias
Plant and be uniformly poured onto in asafoetide foundation portion root tissue, then the soil that plane is opened all is backfilled to into asafoetide foundation portion, gently cover soil,
The asafoetide mushroom liquid strain tieback test that annual spring, two periods of autumn were carried out to first 1 year is investigated；Investigation method is：Recruitment
Have and open the soil plane of asafoetide foundation portion side, clean out in the case where asafoetide foundation portion hoc scenario is not injured and expose asafoetide root,
Observation and the field planting of record asafoetide foundation portion Pleurotus ferulae mycelia and growing state.
In the present invention, described pleuratus ferulae comatus mycelium preculture includes taking out asafoetide mushroom strains from safe, is seeded to
7d～15d is activated on PDA culture medium flat board, with transfer needle picking 0.5cm2～1cm2The pure culture biscuits involvng inoculation of size goes out to high steam
The PD fluid nutrient medium liquid levels of bacterium, keep the mycelia side of bacteria cake upward.22 DEG C～26 DEG C light cultures are subsequently placed in, bacteria cake mycelia is treated
Shaking table culture 3d～7d, shaking table setting speed 180rpm～240rpm are put into after being fully deployed, temperature is 22 DEG C～26 DEG C；It is described
Detection method be：Aseptically, the mycelium suspended liquid of 80 l～150 l of absorption is seeded to PDA culture medium and puts down
Plate, is smoothened with three angle rods for killing bacterium, and in 22 DEG C～26 DEG C 2～5d of light culture, whether observation culture medium flat plate has miscellaneous bacteria.
Preferably, bacteria cake takes top layer subiculum.
In the present invention, the PD fluid nutrient mediums refer to that 80ml～120ml PD plus liquid-rich culture medium are contained in 250mL
In triangular flask, 10～20 sterilized glass marbles are put in advance in triangular flask.
In the present invention, the mycelium suspended liquid prepares and includes being smashed to pieces liquid specific kind with loop-carrier, is seeded to and fills
In the 1000ml triangular flasks of 600ml～800ml PD enriched mediums, cultivate on shaking table, shaking table setting speed 180rpm～
240rpm, temperature is 22 DEG C～26 DEG C.Grow up to after Special seed mycelium carries out quality with after the big slight mycelium pellet of small rice grain to it
Detection；Described detection method is：Aseptically, draw the mycelium suspended liquid of 80 l～150 l and be seeded to PDA cultures
Base flat board, 25 DEG C of light culture 2d～5d see whether polluted bacteria or fungi, choose the mycelium suspended liquid without pollution in 4
DEG C save backup.
Beneficial effects of the present invention:
The method of a kind of asafoetide mushroom liquid strain field inoculation asafoetide plant that the present invention is provided, by asafoetide mushroom liquid strain system
Standby and asafoetide mushroom liquid strain field inoculation asafoetide plant, can effectively recover the side of the Ferula plant roots stem on Gobi desert
Ear belongs to wild mushroom mycelia, for protection wild Pleurotus ferulae resource and recovery Gobi desert ecosystem have great reality meaning
Justice.
Description of the drawings
Fig. 1 is shown as pleuratus ferulae comatus mycelium preculture situation one in the present invention.
Fig. 2 is shown as pleuratus ferulae comatus mycelium preculture situation two in the present invention.
Fig. 3 is shown as asafoetide mushroom liquid strain Special seed in the present invention.
Fig. 4 is shown as asafoetide mushroom liquid strain in the present invention.
Fig. 5 is shown as asafoetide plant root disposition in the present invention.
Fig. 6 is shown as asafoetide mushroom liquid strain tieback situation in the present invention.
Fig. 7 is shown as Pleurotus ferulae mycelia colonisation in the present invention, and 1 represents the Pleurotus ferulae mycelia of field planting in figure.
Specific embodiment
The specific embodiment of the present invention is described in further detail below, but the method for the present invention is not limited to following realities
Apply example.
Embodiment one：The method of asafoetide mushroom liquid strain field inoculation asafoetide plant
The invention provides a kind of method of asafoetide mushroom liquid strain field inoculation asafoetide plant, is made up of following steps：
Step 1：Preculture pleuratus ferulae comatus mycelium and to Detection.
Step 2：The good mycelium of preculture is seeded on the sawdust medium of sterilization treatment, 25 DEG C of light cultures treat bacterium
Filament length is completely liquid specific kind, and to it quality testing is carried out；Described detection method is：Aseptically, liquid is taken special
PDA culture medium flat board is seeded to kind, 25 DEG C of light culture 2d see whether polluted bacteria or fungi, choose the liquid without pollution
Body Special seed prepares mycelium suspended liquid and carries out quality testing, referring to accompanying drawing 3.
Step 3：Mycelium suspended liquid is diluted into 3 times～5 times with clear water, stand-by liquid spawn is after mixing.
Step 4：Asafoetide mushroom liquid strain field inoculation asafoetide plant：
The asafoetide plant without the field planting of Pleurotus ferulae mycelia is selected, asafoetide foundation portion soil spades plane is opened, depth 10cm, not
Expose asafoetide foundation portion in the case of the rotten root in destruction asafoetide foundation portion, take 80ml～120ml asafoetide mushroom liquid strains and uniformly pour
It is poured in asafoetide foundation portion root tissue, then the soil opened of plane is all backfilled to into asafoetide foundation portion, gently covers soil, the annual spring,
The asafoetide mushroom liquid strain tieback test that two periods of autumn were carried out to first 1 year is investigated；Investigation method is：With instrument by asafoetide
The soil plane of foundation portion side is opened, and is cleaned out in the case where asafoetide foundation portion hoc scenario is not injured and is exposed asafoetide root, is observed and is remembered
The field planting of record asafoetide foundation portion Pleurotus ferulae mycelia and growing state.
In the present invention, described pleuratus ferulae comatus mycelium preculture includes taking out asafoetide mushroom strains from safe, is seeded to
7d～15d is activated on PDA culture medium flat board, with transfer needle picking 0.5cm2～1cm2The pure culture biscuits involvng inoculation of size goes out to high steam
The PD fluid nutrient medium liquid levels of bacterium, keep the mycelia side of bacteria cake upward.22 DEG C～26 DEG C light cultures are subsequently placed in, bacteria cake mycelia is treated
Shaking table culture 3d～7d, shaking table setting speed 180rpm～240rpm are put into after being fully deployed, temperature is 22 DEG C～26 DEG C；It is described
Detection method be：Aseptically, the mycelium suspended liquid of 80 l～150 l of absorption is seeded to PDA culture medium and puts down
Plate, is smoothened with three angle rods for killing bacterium, and in 22 DEG C～26 DEG C 2～5d of light culture, whether observation culture medium flat plate has miscellaneous bacteria, referring to
Accompanying drawing 1 and accompanying drawing 2.
Preferably, bacteria cake takes top layer subiculum.
In the present invention, the PD fluid nutrient mediums refer to that 80ml～120ml PD plus liquid-rich culture medium are contained in 250mL
In triangular flask, 10～20 sterilized glass marbles are put in advance in triangular flask.
In the present invention, the mycelium suspended liquid prepares and includes being smashed to pieces liquid specific kind with loop-carrier, is seeded to and fills
In the 1000ml triangular flasks of 600ml～800ml PD enriched mediums, cultivate on shaking table, shaking table setting speed 180rpm～
240rpm, temperature is 22 DEG C～26 DEG C.Grow up to after Special seed mycelium carries out quality with after the big slight mycelium pellet of small rice grain to it
Detection；Described detection method is：Aseptically, draw the mycelium suspended liquid of 80 l～150 l and be seeded to PDA cultures
Base flat board, 25 DEG C of light culture 2d～5d see whether polluted bacteria or fungi, choose the mycelium suspended liquid without pollution in 4
DEG C save backup, referring to accompanying drawing 4.
Embodiment two：It is prepared by asafoetide mushroom liquid strain
In the present invention, described pleuratus ferulae comatus mycelium preculture includes taking out asafoetide mushroom strains from safe, is seeded to PDA trainings
7d is activated on foster base flat board, with transfer needle picking 0.5cm2The PD fluid nutrient mediums of the pure culture biscuits involvng inoculation of size to high pressure steam sterilization
Liquid level, keeps the mycelia side of bacteria cake upward.22 DEG C of light cultures are subsequently placed in, after bacteria cake mycelia is fully deployed shaking table culture is put into
3d, shaking table setting speed 180rpm, temperature is 22 DEG C；Described Detection method is：Aseptically, 80 l bacterium are drawn
Mycelial suspension is seeded to PDA culture medium flat board, is smoothened with three angle rods for killing bacterium, in 22 DEG C of light culture 2d, observes culture medium
Whether flat board has miscellaneous bacteria, referring to accompanying drawing 1 and accompanying drawing 2.
Preferably, bacteria cake takes top layer subiculum.
In the present invention, the PD fluid nutrient mediums refer to that the PD plus liquid-rich culture medium of 80ml is contained in 250mL triangular flasks
In, 10 sterilized glass marbles are put in advance in triangular flask.
In the present invention, sawdust medium is prepared, after sterilization treatment, the good mycelium suspension inoculation of preculture to wood chip is trained
On foster base, 22 DEG C of light cultures treat that mycelia is covered with as liquid specific kind；Quality testing is carried out to it, detection method is：Aseptic
Under the conditions of, taking liquid specific kind and be seeded to PDA culture medium flat board, 22 DEG C of light culture 2d see whether polluted bacteria or fungi, choosing
Take the liquid specific kind not polluted to save backup in 4 DEG C, referring to accompanying drawing 3.
In the present invention, the mycelium suspended liquid is prepared and quality testing includes being smashed to pieces liquid specific kind with loop-carrier,
In being seeded to the 1000ml triangular flasks for filling 600ml PD enriched mediums, cultivate on shaking table, shaking table setting speed 180rpm,
Temperature is 22 DEG C.Quality testing, detection side are carried out to it after the little mycelium pellet that Special seed mycelium grows up to small rice grain size
Method is：Aseptically, draw the mycelium suspended liquid of 80 l and be seeded to PDA culture medium flat board, 25 DEG C of light culture 2d, observation is
No polluted bacteria or fungi, choose the mycelium suspended liquid without pollution and save backup in 4 DEG C, referring to accompanying drawing 4.
Embodiment three：It is prepared by asafoetide mushroom liquid strain
In the present invention, described pleuratus ferulae comatus mycelium preculture includes taking out asafoetide mushroom strains from safe, is seeded to PDA trainings
15d is activated on foster base flat board, with transfer needle picking 1cm2The PD fluid nutrient mediums of the pure culture biscuits involvng inoculation of size to high pressure steam sterilization
Liquid level, keeps the mycelia side of bacteria cake upward.26 DEG C of light cultures are subsequently placed in, after bacteria cake mycelia is fully deployed shaking table culture is put into
7d, shaking table setting speed 240rpm, temperature is 26 DEG C；Described Detection method is：Aseptically, 150 l are drawn
Mycelium suspended liquid is seeded to PDA culture medium flat board, is smoothened with three angle rods for killing bacterium, in 26 DEG C of light culture 5d, observation culture
Whether base flat board has miscellaneous bacteria, referring to accompanying drawing 1 and accompanying drawing 2.
Preferably, bacteria cake takes top layer subiculum.
In the present invention, the PD fluid nutrient mediums refer to that the PD plus liquid-rich culture medium of 120ml is contained in 250mL triangles
In bottle, 20 sterilized glass marbles are put in advance in triangular flask.
In the present invention, sawdust medium is prepared, after sterilization treatment, the good mycelium suspension inoculation of preculture to wood chip is trained
On foster base, 26 DEG C of light cultures treat that mycelia is covered with as liquid specific kind；Quality testing is carried out to it, detection method is：Aseptic
Under the conditions of, taking liquid specific kind and be seeded to PDA culture medium flat board, 26 DEG C of light culture 5d see whether polluted bacteria or fungi, choosing
Take the liquid specific kind not polluted to save backup in 4 DEG C, referring to accompanying drawing 3.
In the present invention, the mycelium suspended liquid is prepared and quality testing includes being smashed to pieces liquid specific kind with loop-carrier,
In being seeded to the 1000ml triangular flasks for filling 800ml PD enriched mediums, cultivate on shaking table, shaking table setting speed 240rpm,
Temperature is 26 DEG C.Grow up to after Special seed mycelium carries out quality testing, detection method with after the big slight mycelium pellet of small rice grain to it
For：Aseptically, draw the mycelium suspended liquid of 150 l and be seeded to PDA culture medium flat board, 25 DEG C of light culture 5d, observation is
No polluted bacteria or fungi, choose the mycelium suspended liquid without pollution and save backup in 4 DEG C, referring to accompanying drawing 4.
Example IV：It is prepared by asafoetide mushroom liquid strain
In the present invention, described pleuratus ferulae comatus mycelium preculture includes taking out asafoetide mushroom strains from safe, is seeded to PDA trainings
10d is activated on foster base flat board, with transfer needle picking 0.8cm2The PD Liquid Cultures of the pure culture biscuits involvng inoculation of size to high pressure steam sterilization
Base fluid face, keeps the mycelia side of bacteria cake upward.25 DEG C of light cultures are subsequently placed in, shaking table training is put into after bacteria cake mycelia is fully deployed
Foster 5d, shaking table setting speed 210rpm, temperature is 24 DEG C；Described Detection method is：Aseptically, 100 are drawn
The mycelium suspended liquid of l is seeded to PDA culture medium flat board, is smoothened with three angle rods for killing bacterium, in 25 DEG C of light culture 3d, observation training
Whether foster base flat board has miscellaneous bacteria, referring to accompanying drawing 1 and accompanying drawing 2.
Preferably, bacteria cake takes top layer subiculum.
In the present invention, the PD fluid nutrient mediums refer to that 80ml～120ml PD plus liquid-rich culture medium are contained in 250mL
In triangular flask, 15 sterilized glass marbles are put in advance in triangular flask.
In the present invention, sawdust medium is prepared, after sterilization treatment, the good mycelium suspension inoculation of preculture to wood chip is trained
On foster base, 24 DEG C of light cultures treat that mycelia is covered with as liquid specific kind；Quality testing is carried out to it, detection method is：Aseptic
Under the conditions of, taking liquid specific kind and be seeded to PDA culture medium flat board, 23 DEG C of light culture 4d see whether polluted bacteria or fungi, choosing
Take the liquid specific kind not polluted to save backup in 4 DEG C, referring to accompanying drawing 3.
In the present invention, the mycelium suspended liquid is prepared and quality testing includes being smashed to pieces liquid specific kind with loop-carrier,
In being seeded to the 1000ml triangular flasks for filling 700ml PD enriched mediums, cultivate on shaking table, shaking table setting speed 210rpm,
Temperature is 23 DEG C.Grow up to after Special seed mycelium carries out quality testing, detection method with after the big slight mycelium pellet of small rice grain to it
For：Aseptically, draw the mycelium suspended liquid of 110 l and be seeded to PDA culture medium flat board, 25 DEG C of light culture 3d, observation is
No polluted bacteria or fungi, choose the mycelium suspended liquid without pollution and save backup in 4 DEG C, referring to accompanying drawing 4.
Embodiment five：Asafoetide mushroom liquid strain field inoculation asafoetide plant
In the present invention, the selection of host's asafoetide simultaneously includes selecting the asafoetide without the field planting of Pleurotus ferulae mycelia to plant to root process
Thing, asafoetide foundation portion soil spades plane is opened, and depth 5cm exposes Ah in the case where the rotten root in asafoetide foundation portion is not destroyed
Wei's root base portion, referring to accompanying drawing 5.
In the present invention, the asafoetide root liquid spawn tieback and root are processed and refer to that to take 80ml asafoetide mushroom liquid strains equal
It is even to be poured onto in asafoetide foundation portion root tissue, then the soil that plane is opened all is backfilled to into asafoetide foundation portion, soil is gently covered, referring to
Accompanying drawing 6.
It is described that tieback test in asafoetide mushroom liquid strain field is carried out by investigation records refers to annual spring, autumn two in the present invention
The asafoetide mushroom liquid strain tieback test that individual period was carried out to first 1 year is investigated；Investigation method is：With instrument by asafoetide foundation
Portion side soil plane open, clean out in the case where asafoetide foundation portion hoc scenario is not injured and expose asafoetide root, observation and record Ah
The field planting of Wei root base portion Pleurotus ferulae mycelia and growing state, referring to accompanying drawing 7.
Embodiment six：Asafoetide mushroom liquid strain field inoculation asafoetide plant
In the present invention, the selection of host's asafoetide simultaneously includes selecting the asafoetide without the field planting of Pleurotus ferulae mycelia to plant to root process
Thing, asafoetide foundation portion soil spades plane is opened, and depth 15cm exposes Ah in the case where the rotten root in asafoetide foundation portion is not destroyed
Wei's root base portion, referring to accompanying drawing 5.
In the present invention, the asafoetide root liquid spawn tieback and root process are referred to and take 120ml asafoetide mushroom liquid strains
Uniformly it is poured onto in asafoetide foundation portion root tissue, then the soil that plane is opened all is backfilled to into asafoetide foundation portion, gently cover soil, joins
See accompanying drawing 6.
It is described that tieback test in asafoetide mushroom liquid strain field is carried out by investigation records refers to annual spring, autumn two in the present invention
The asafoetide mushroom liquid strain tieback test that individual period was carried out to first 1 year is investigated；Investigation method is：With instrument by asafoetide foundation
Portion side soil plane open, clean out in the case where asafoetide foundation portion hoc scenario is not injured and expose asafoetide root, observation and record Ah
The field planting of Wei root base portion Pleurotus ferulae mycelia and growing state, referring to accompanying drawing 7.
Embodiment seven：The method feasibility analysis of asafoetide mushroom liquid strain field inoculation asafoetide plant
Asafoetide mushroom liquid strain field inoculation asafoetide vegetal tests, have continuously done for many years, and annual inoculation selects May in spring, its
In, inoculation quantity is 240 plants within 2014, is within 2015 250 plants, is within 2016 350 plants.
The test being inoculated with then is investigated in autumn September part then, and investigation result shows：Pleurotus ferulae mycelia in 2014 into
Work(field planting rate is 83.82%, 2015 successfully field planting rate be 64.28%, 2016 successfully field planting rate be 66.10%, it is averagely successfully fixed
Plant rate is 72.59%.
Result of the test shows that using asafoetide mushroom liquid strain field inoculation asafoetide plant method Pleurotus ferulae can be effectively improved
Field planting rate of the mycelia in wild asafoetide plant root.
In China, its wild resource is distributed only on the Gobi desert Resina Ferulae beach of Junggar Basin, Xinjiang, china edge awful weather, and
Selective rhizome portion that is saprophytic or colonizing in Chinese medicine asafoetide, abundance is rare.In recent years, due to environment and human factor, asafoetide
Mushroom wild resource recoverable amount drastically declines, and the Pleurotus ferulae resource in some regions almost disappears, it would be highly desirable to protect.Meanwhile, it is domestic at present
The cultivated strains for producing for many years are all from the separation of Xinjiang Wild fructification, and the Pleurotus ferulae of country's cultivation in recent years has bacterial strain and mixes
The problems such as random, unstable kind property, spawn degeneration, is also required to wild Pleurotus ferulae breed breeding resource.But Pleurotus ferulae field grown ring
Border is severe, and fertility is low, and natural breed survives low, asafoetide mushroom liquid strain field inoculation is returned into asafoetide plant and is even more required
It is harsh.Activity prepared by asafoetide mushroom liquid strain has vital impact, conventional method to asafoetide mushroom strains field inoculation
It is unstable also to there is kind of property in the Pleurotus ferulae thalline of preparation in the environment for manually providing, the problem of spawn degeneration, if by its
Be inoculated into be even more in field extreme environment and be difficult to survive, the depth that is inoculated with during field inoculation and asafoetide mushroom liquid strain number all
Field planting rate, result of study of the present invention can be affected to show, depth it is too deep or it is excessively shallow can all cause field planting rate to reduce at double, during inoculation
It is also that field planting rate is relatively low that asafoetide mushroom liquid strain is very few, and asafoetide mushroom liquid strain excessively also occurs that inoculation is successfully dead year by year
Situation, can not realize recovering the purpose of field Pleurotus ferulae resource.The technical scheme that the present invention is provided by acclimatization culture or
The secondary cultivation of wild asafoetide mushroom strains is inoculated into person the root of field asafoetide plant, good to protecting Pleurotus ferulae germ plasm resource to have
Good effect, with field planting rate of the present invention, as long as increasing inoculum concentration, quickly wild Pleurotus ferulae resource can return to number several years ago again
Amount.
As mentioned above, you can preferably realize the present invention, the above embodiments are only the side of being preferable to carry out to the present invention
Formula is described, and not the scope of the present invention is defined, and on the premise of without departing from design spirit of the present invention, this area is general
Various modifications and improvement that logical technical staff makes to technical scheme, all should fall into present invention determine that protection domain
It is interior.
Claims (5)
1. a kind of method of asafoetide mushroom liquid strain field inoculation asafoetide plant, it is characterised in that be made up of following steps：
Step 1：Preculture pleuratus ferulae comatus mycelium and to Detection；
Step 2：The good mycelium of preculture is seeded on the sawdust medium of sterilization treatment, 22 DEG C～26 DEG C light cultures are treated
Mycelia is covered with as liquid specific kind, and to it quality testing is carried out；Described detection method is：Aseptically, liquid is taken
Special seed is seeded to PDA culture medium flat board, and 22 DEG C～26 DEG C light culture 2d～5d see whether polluted bacteria or fungi, chooses
Liquid specific kind without pollution prepares mycelium suspended liquid and carries out quality testing；
Step 3：Mycelium suspended liquid is diluted into 3 times～5 times with clear water, stand-by liquid spawn is after mixing；
Step 4：Asafoetide mushroom liquid strain field inoculation asafoetide plant：The asafoetide plant without the field planting of Pleurotus ferulae mycelia is selected, will
Asafoetide foundation portion soil spades plane is opened, and depth 5cm～15cm exposes Ah in the case where the rotten root in asafoetide foundation portion is not destroyed
Wei's root base portion, takes 80ml～120ml asafoetides mushroom liquid strain and is uniformly poured onto in asafoetide foundation portion root tissue, then opens plane
Soil is all backfilled to asafoetide foundation portion, gently covers soil, and Second Year spring and autumn are with instrument by the soil of asafoetide foundation portion side
Earth plane is opened, and is cleaned out in the case where asafoetide foundation portion hoc scenario is not injured and is exposed asafoetide root, observation and record asafoetide foundation portion Ah
The field planting of Wei mushroom mycelia and growing state.
2. a kind of method of asafoetide mushroom liquid strain field inoculation asafoetide plant as claimed in claim 1, it is characterised in that institute
The pleuratus ferulae comatus mycelium preculture stated includes taking out asafoetide mushroom strains from safe, is seeded on PDA culture medium flat board and activates
7d～15d, with transfer needle picking 0.5cm2～1cm2The PD Liquid Culture base fluids of the pure culture biscuits involvng inoculation of size to high pressure steam sterilization
Face, keeps the mycelia side of bacteria cake upward, is subsequently placed in 22 DEG C～26 DEG C light cultures, and after bacteria cake mycelia is fully deployed shaking table is put into
Culture 3d～7d, shaking table setting speed 180rpm～240rpm, temperature is 22 DEG C～26 DEG C；Described Detection method is：
Aseptically, draw the mycelium suspended liquid of 80 l～150 l and be seeded to PDA culture medium flat board, with three angle rods for killing bacterium
Smoothen, in 22 DEG C～26 DEG C light culture 2d～5d, whether observation culture medium flat plate has miscellaneous bacteria.
3. a kind of method of asafoetide mushroom liquid strain field inoculation asafoetide plant as claimed in claim 1, it is characterised in that institute
Stating mycelium suspended liquid and preparing includes being smashed to pieces liquid specific kind with loop-carrier, is seeded to and fills 600ml～800ml PD and add richness
In the 1000ml triangular flasks of culture medium, cultivate on shaking table, shaking table setting speed 180rpm～240rpm, temperature is 22 DEG C～26
DEG C, grow up to after Special seed mycelium carries out quality testing with after the big slight mycelium pellet of small rice grain to it；Described detection method is：
Aseptically, draw the mycelium suspended liquid of 80 l～150 l and be seeded to PDA culture medium flat board, 25 DEG C of light culture 2d～5d,
Polluted bacteria or fungi are seen whether, the mycelium suspended liquid without pollution is chosen and is saved backup in 4 DEG C.
4. a kind of method of asafoetide mushroom liquid strain field inoculation asafoetide plant as claimed in claim 2, it is characterised in that bacterium
Cake takes top layer subiculum.
5. a kind of method of asafoetide mushroom liquid strain field inoculation asafoetide plant as claimed in claim 2, it is characterised in that institute
State PD fluid nutrient mediums and refer to that 80ml～120ml PD plus liquid-rich culture medium are contained in 250mL triangular flasks, carry in triangular flask
Before be put into 10～20 sterilized glass marbles.
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| CN110278828A (en) * | 2019-07-10 | 2019-09-27 | 新疆农业科学院植物保护研究所 | A kind of method of Pleurotus nebrodensis field child care on the spot |
| CN110278827A (en) * | 2019-07-10 | 2019-09-27 | 新疆农业科学院植物保护研究所 | A kind of method of Pleurotus nebrodensis field child care fruiting on the spot |
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| CN110278827A (en) * | 2019-07-10 | 2019-09-27 | 新疆农业科学院植物保护研究所 | A kind of method of Pleurotus nebrodensis field child care fruiting on the spot |
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