CN107629967A - Fluid nutrient medium and rhizoma Gastrodiae implantation methods for the Germination Strain of rhizoma Gastrodiae plantation - Google Patents
Fluid nutrient medium and rhizoma Gastrodiae implantation methods for the Germination Strain of rhizoma Gastrodiae plantation Download PDFInfo
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Abstract
The present invention relates to the fluid nutrient medium for the Germination Strain planted for rhizoma Gastrodiae and rhizoma Gastrodiae implantation methods.Specifically, the fluid nutrient medium of the Germination Strain for rhizoma Gastrodiae plantation, is wherein included in terms of every 250mL：8~12 grams of potato, 8~12 grams of wheat bran, 8~12 grams of dregs of beans, 4~6 grams of white sugar, 0.5~1 gram of peptone, 0.4~0.6 gram of potassium dihydrogen phosphate, 0.2~0.3 gram of magnesium sulfate, 0.8~1.2 milligram of thiamine, appropriate amount of water add to 250mL.In addition, present invention also offers rhizoma Gastrodiae implantation methods, the step of it is including the use of Germination Strain fluid nutrient medium culture Germination Strain and using halimasch liquid medium culture halimasch.Germination Strain fluid nutrient medium of the present invention have excellent properties for example its can obtain high mycelial growth rate.
Description
Technical field
The present invention relates to rhizoma Gastrodiae planting technology field, is related to Germination Strain and honey required for gastrodia seed germination and rhizoma Gastrodiae growth
The culture medium of ring bacterium, the fluid nutrient medium used more particularly to such Germination Strain and halimasch.Particularly the present invention relates to
Rhizoma Gastrodiae implantation methods are carried out in the fluid nutrient medium of the Germination Strain of rhizoma Gastrodiae plantation, and using the fluid nutrient medium.
Background technology
Rhizoma Gastrodiae (scientific name：Gastrodia elata Bl.), also known as rhizoma gastrodiae, solely shake sesame, from it is female, close and superintended and directed from grass, god's grass, ghost
Postal, Mokpo, RHIZONA GASTRODIAE, DINGFENGCAO, Bai Longpi etc., it is orchid family Gastrodia herbaceos perennial.Root-like stock is plump, no greenery,
Capsule obovate elliptic, often with stem tuber or seminal propagation.Its rhizome be used as medicine to treat have a dizzy spell, extremity numbness, children
The diseases such as infantile convulsion, it is rare Chinese medicine, treatment headache and insomnia is shared with fine jade treasure ganoderma lucidum.
Gastrodia seed germination and rhizoma Gastrodiae growth are, it is necessary to which Germination Strain and halimasch offer nutrition could sprout and grow.Due to
Rhizoma Gastrodiae unrooted, the blade without assimilation, so itself can not directly absorb the nutrition in environment.The basic battalion of rhizoma Gastrodiae growth
Foster source is halimasch, and no halimasch rhizoma Gastrodiae cannot grow.But the relation between rhizoma Gastrodiae and halimasch is extremely special, multiple
It is miscellaneous.There is digestion and the nutrition relationship being digested between them.Rhizoma Gastrodiae and halimasch are living together, and show mutually interdependent
Deposit, the symbiosis of mutual reciprocity and mutual benefit.This relation changes with the change of growth phase and environmental condition.
The nutrition foundation that " trees " are halimasch with rhizoma Gastrodiae growth, halimasch is a kind of parasitical fungi, can be in nature
Survived on more than 600 kinds of trees or herbaceous plant.It is not only parasitic on tree living, grass roots, and can be on the root and stem extremely set
Procreation.Rhizoma Gastrodiae does not manufacture the ability of nutriment due to itself, it is necessary to is grown by halimasch as source of nutrition.Rhizoma Gastrodiae --- honey
Constituted between ring bacterium --- trees (green plants) one " food chain ".Halimasch is " nutrition bridge " therein, and trees are
Material base between them.The theoretical foundation of rhizoma Gastrodiae GAP cultivations is exactly the understanding to relation between their threes.
Halimasch invades the condition of Gastrodia Elata Stem Tuber, after the halimasch shoestring grown touches rhizoma Gastrodiae, shoestring branch point
The growing point intrusion rhizoma Gastrodiae protocorm or stem tuber, rhizoma Gastrodiae at end are combined from that point on the symbiosis of halimasch.When halimasch shoestring is given birth to
Long point penetrates numb body surface skin, and the mycelium of pencil enters cortex, starts in a manner of scattered mycelia by space between cells or cell
Wall pit enters cell interior, nucleus is changed, and is decomposed after first becoming big, organelle and polyoses grain etc. disappear, nutrition
Material is absorbed by mycelium.This process is generally referred to as halimasch intake Gastrodiac nutritive period.Halimasch invades Gastrodia Elata Stem Tuber
Condition be that Gastrodia Elata Stem Tuber is in dormancy or sprouts the stage, and halimasch shoestring be in yellow-white or brownish red, halimasch is in young tender
Phase.The aging shoestring of dark brown can not invade Gastrodia Elata Stem Tuber, and the Gastrodia Elata Stem Tuber in peak of growing season or new tuberization are not in addition
Can be by halimasch intrusion.
Rhizoma Gastrodiae has the ability of assimilation resolution armillaria mycelium.In rhizoma Gastrodiae underground stem tuber close in the tissue at center pillar position,
There is the cell that ordered series of numbers build is larger, viability is stronger, there is bacteriolysis, the function of having assimilation resolution armillaria mycelium, claim
To digest layer.When armillaria mycelium progresses into the cell interior of cortex depths, it will be surrounded, twist together into by plasm
Group, and gradually expand and by rhizoma Gastrodiae cell disintegration.Thereafter digest confluent monolayer cells and significantly become big, plasm is thick, the mycelium of invasion
Assimilation is resolved, turns into the nutriment of rhizoma Gastrodiae growth, and the hemp of uncombined halimasch is in then " starvation ".
Rhizoma Gastrodiae, with growing for rhizoma Gastrodiae, is put forth in arrow fiber crops to the feedback aspect of halimasch nutrition and blooms or plant Taro Aso
After going out sub- fiber crops, raw ramie body (kind fiber crops) gradual aging, the ability for dissolving halimasch is lost, at this moment halimasch occupy advantage, just in day
The internal amount reproduction of fiber crops, its nutrition is drawn, raw ramie body has reformed into the nutrient source of halimasch, and last stem tuber is hollow to rot.This
Stage shows as parasitism of the halimasch to rhizoma Gastrodiae.
In summary, rhizoma Gastrodiae is to invade its cortex and the intracellular halimasch bacterium of center pillar by resolution under normal circumstances
Silk is made source of nutrition and grown, and this is the aspect that rhizoma Gastrodiae relies on close ring bacterium.And once drought and waterlogging, high temperature, low temperature etc. occur for environment
When factor changes, rhizoma Gastrodiae physiological function and growth tendency can be caused to weaken.Therefore the relation of rhizoma Gastrodiae and halimasch also occurs unfavorable
In the transformation of rhizoma Gastrodiae, when being reduced to less than 15 DEG C such as the later stage temperature of rhizoma Gastrodiae growth, rhizoma Gastrodiae will enter rest period, but warm herein
Under degree, halimasch can still grow.In GAP cultivations, watering or rainfall are excessive, and halimasch growing way is excessively prosperous, endangers rhizoma Gastrodiae in turn,
Putrefactive phenomenon can occur for newborn sinks.As can be seen here, the symbiosis between rhizoma Gastrodiae and halimasch with different growing stage and
The change of environmental condition and change.Suitable environmental condition and reasonable cultivation technique are only created or utilized, can be only achieved
Expected breeding objectives.
Rhizoma Gastrodiae passes through generative propagation, i.e. seminal propagation.This is to prevent one of main path of gastrodia tuber from degradation.Seeds of Gastrodia elata is micro-
It is small, no endosperm, only it is made up of archiblast.Its sprouting infects embryo by Mycena (Mycena) a kind of hypha,hyphae and provides battalion
Support, this kind of fungi that we are referred to as promotion gastrodia seed germination is gastrodia elata seed Germination bacterium.
Document report gastrodia elata seed Germination bacterium belongs to Mycena (Mycena) fungi, and this Germination Strain partly should
For indoors, the experiment of outdoor gastrodia seed germination and Gastrodia plant sub- sprouting and promote production.Japanese wasteland person of outstanding talent help once in
" rhizoma Gastrodiae and halimasch symbiosis " text has been delivered within 1911, thereafter it is believed that rhizoma Gastrodiae is in whole growth cycle, has all been be unable to do without
Halimasch.To be studied according to Xu Jintang etc., halimasch can establish good symbiosis with Gastrodia Elata Stem Tuber, and nutrition is provided for rhizoma Gastrodiae, but
Sprouting to seeds of Gastrodia elata does not have facilitation not only, there is inhibitory action on the contrary.They are from the protocorm of gastrodia seed germination
The fungies such as Mycen osmundicola are isolated, sprouting of these fungies to seeds of Gastrodia elata has facilitation.Experiment shows that seeds of Gastrodia elata is
By symbiotic germination fungi mycelia infect give rhizoma Gastrodiae embryo provide nutrition.Gastrodia elata seed Germination bacterium has been studied must compare it is deep
It is its purple small mushroom and dendrobium mixture.Germination Strain one kind fungi, it is how saprophytic on high mountain woodland fallen leaves, strong point is known as to fiber
Solution ability, autumn and winter leaf come off in ground, contact the rotten leaf of deadwood of native table moisturizing, and Germination Strain can invade the vital day of tool again
In numb seed, make seed symbiotic germination, therefore Germination Strain one kind fungi, Major Nutrient type are saprogenesises, but also have facultative post
Raw characteristic.
Seeds of Gastrodia elata symbiotic germination bacterium, it is a kind of aerobic fungi, they are mainly distributed on woodland dry branches and fallen leaves in forest
In layer and topsoil, found in incubation, if compost overfills or bottle cap is too tight, influence whether this kind of bacterium
Growth, the speed of growth delay, incubation time length.Germination Strain is a kind of weak parasite, and it is thin that they can only infect Gastrodia blastema portion
Born of the same parents.The protocorm for infecting seeds of Gastrodia elata germination and vegetative propagation corm, rice fiber crops, hemp is also not observed.When halimasch invades original
After the vegetative propagation corm that bulb differentiates, Germination Strain and halimasch are simultaneously present in same vegetative propagation corm, and it is to day
The trophism of fiber crops is gradually replaced by halimasch.Germination Strain can grow in 15 DEG C of -30 DEG C of temperature ranges.With 25 DEG C of mycelia
Growth it is most fast, less than 20 DEG C or higher than 25 DEG C mycelial growth rates substantially subtract it is unrestrained.
It is increasing to the demand of Germination Strain and halimasch with the fast development that rhizoma Gastrodiae is planted, and traditional solid
Its production cycle of production technology is long, production cost is high, production is controlled by season, high strain transfer algebraically is difficult control strain matter
The unfavorable factor such as amount, serious waste of resources seriously constrains the development of strain industry so as to influence the development of rhizoma Gastrodiae plantation industry.
Strain cultivation technology is quickly grown in the application of strain industry in recent years, and its major reason is exactly to overcome conventional solid bacterium
The unfavorable factor of kind of production, therefore study application of the strain cultivation technology in halimasch and Germination Strain production and just seem pole
Its is important, and the key element in the fluid nutrient medium being adapted to during liquid spawn produces is production process.
Therefore, the Liquid Culture based formulas for being used for halimasch and Germination Strain in rhizoma Gastrodiae plantation is still expected to have in this area,
And expect that this formula has excellent culture performance.
Bibliography：
【1】Xu Jin hall China gastrodin cultivation [M] Beijing：China Concord Medical Science University of Beijing Medical University combined publication
Society, 1993：1-99.
【2】Cao Debin：Rhizoma Gastrodiae, ganoderma lucidum, Cordceps militaris, Stropharia rugoso-annulata production technology question and answer.
【3】Cao Debin, Wang Guangrong, correction are faced：Edible mushroom basis and seeding technique question and answer.
【4】Original botanical investigation and identification [J] the R＆D of modern TCM of Li Dexun, Zhang Guanmin, Ma Yuexin Zhaotongs rhizoma Gastrodiae with
Practice, 2005,19 (1)：29-31.
【5】Progress [J] Chinese herbal medicines of Yang Shilin, Lan Jin, Xu Jin hall rhizoma Gastrodiaes, 2000,3 (1)：66-69.
【6】Xu Jintang, Guo Shunxing, Fan Li, wait research [J] the fungus system of seeds of Gastrodia elata and fungus Mycenae sp symbiotic germination
System, 2001,20 (1)：137-141.
【7】Guo Shunxing, Wang Qiu grain husk promote the dendrobium mixture strain excellent characteristic and effect [J] fungus of gastrodia seed germination
System, 2001,20 (3)：408-412.
【8】Lin Jing, Zhao Ping, He Lili, wait design [J] agricultural research of Culture Container for Liquid Fungus Seed of Edible Fungus, and 2007
(9)：105-108
【9】Lv Zuozhou, Lv Xiaojuan, Li Weimin：Edible mushroom 300 is asked.
【10】Zhang Shengyou：Chinese liquid spawn production new technology.
The content of the invention
It is an object of the invention to provide a kind of Liquid Culture basigamy for being used for halimasch and Germination Strain in rhizoma Gastrodiae plantation
Side, and expect that this formula has excellent culture performance.Have now surprisingly been found that there is feature of present invention formula
It is used for the fluid nutrient medium of halimasch and Germination Strain in rhizoma Gastrodiae plantation, excellent technique effect as described herein is presented.
Therefore, in the first aspect of the present invention, there is provided a kind of fluid nutrient medium of halimasch for rhizoma Gastrodiae plantation, with
Per 250mL, meter wherein includes：8~12 grams of corn flour, 8~12 grams of wheat bran, 5.5~7 grams of dregs of beans, 4~6 grams of white sugar, peptone 0.5
~1 gram, 0.4~0.6 gram of potassium dihydrogen phosphate, 0.2~0.3 gram of magnesium sulfate, 0.8~1.2 milligram of thiamine, appropriate amount of water add to
250mL。
According to the fluid nutrient medium for being used for the halimasch that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Corn
9~11 grams of powder, 9~11 grams of wheat bran, 6~6.5 grams of dregs of beans, 4.5~5.5 grams of white sugar, 0.65~0.85 gram of peptone, biphosphate
0.45~0.55 gram of potassium, 0.225~0.275 gram of magnesium sulfate, 0.9~1.1 milligram of thiamine, appropriate amount of water add to 250mL.
According to the fluid nutrient medium for being used for the halimasch that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Corn
10 grams of powder, 10 grams of wheat bran, 6.25 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate,
1 milligram of thiamine, appropriate amount of water add to 250mL.
According to the fluid nutrient medium for being used for the halimasch that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Potato
38 grams, 2 grams of wheat bran, 5 grams of white sugar, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, 1 milligram of thiamine, water
Add to 250mL in right amount.
According to the fluid nutrient medium for being used for the halimasch that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Potato
25 grams, 12 grams of corn flour, 2.5 grams of white sugar, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, the milli of thiamine 1
Gram, appropriate amount of water add to 250mL.
According to the fluid nutrient medium for being used for the halimasch that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Potato
25 grams, 6 grams of dregs of beans, 2.5 grams of white sugar, 2.5 grams of brown sugar, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, sulphur
1 milligram of amine element, appropriate amount of water add to 250mL.
According to the fluid nutrient medium for being used for the halimasch that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Potato
25 grams, 11 grams of wheat bran, 3.7 grams of white sugar, 3.7 grams of brown sugar, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate,
1 milligram of thiamine, appropriate amount of water add to 250mL.
According to the fluid nutrient medium for being used for the halimasch that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Dregs of beans
7.5 grams, 3.7 grams of wood chip, 2.5 grams of white sugar, 2.5 grams of brown sugar, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, magnesium sulfate 0.25
Gram, 1 milligram of thiamine, appropriate amount of water add to 250mL.
According to the fluid nutrient medium for being used for the halimasch that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Potato
30 grams, 6 grams of wheat bran, 2.5 grams of white sugar, 5 grams of wood chip, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, thiamines
1 milligram of element, appropriate amount of water add to 250mL.
According to the fluid nutrient medium for being used for the halimasch that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Potato
25 grams, 12 grams of wheat bran, 10 grams of corn flour, 5 grams of white sugar, 5 grams of wood chip, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, magnesium sulfate
0.25 gram, 1 milligram of thiamine, appropriate amount of water add to 250mL.
According to the fluid nutrient medium for being used for the halimasch that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Potato
25 grams, 6 grams of dregs of beans, 2.5 grams of white sugar, 2.5 grams of brown sugar, 10 grams of wood chip, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, magnesium sulfate
0.25 gram, 1 milligram of thiamine, appropriate amount of water add to 250mL.
According to the fluid nutrient medium for being used for the halimasch that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Wheat bran
12 grams, 10 grams of dregs of beans, 5 grams of wood chip, 2.5 grams of white sugar, 2.5 grams of brown sugar, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, magnesium sulfate
0.25 gram, 1 milligram of thiamine, appropriate amount of water add to 250mL.
According to the fluid nutrient medium for being used for the halimasch that rhizoma Gastrodiae is planted of the present invention, 2.5 are wherein also included in terms of every 250mL
Gram maltose and 0.05 gram of copper chloride.It has been had now surprisingly been found that, on added in the fluid nutrient medium to halimasch simultaneously
When the maltose and copper chloride of the amount of stating, mycelia dry mass can be significantly improved.
According to the fluid nutrient medium for being used for the halimasch that rhizoma Gastrodiae is planted of the present invention, it is to be prepared via a method which to obtain
's：Each solid material is weighed, adds appropriate amount of water (ordinarily be about 70~80% with liquid product), stirs, boil 20~30 points
Clock, add water to prescribed volume, continue to boil 10 minutes, be dispensed into conical flask, every bottle of 125mL, 5 glass are added in each bottle
Glass ball, is then sealed with cotton, then 121 degree of autoclavings 30 minutes in autoclave, is produced.
Further, second aspect of the present invention provides a kind of fluid nutrient medium of the Germination Strain for rhizoma Gastrodiae plantation, with
Per 250mL, meter wherein includes：8~12 grams of potato, 8~12 grams of wheat bran, 8~12 grams of dregs of beans, 4~6 grams of white sugar, peptone 0.5~1
Gram, 0.4~0.6 gram of potassium dihydrogen phosphate, 0.2~0.3 gram of magnesium sulfate, 0.8~1.2 milligram of thiamine, appropriate amount of water add to 250mL.
According to the fluid nutrient medium for being used for the Germination Strain that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Potato
9~11 grams, 9~11 grams of wheat bran, 9~11 grams of dregs of beans, 4.5~5.5 grams of white sugar, 0.65~0.85 gram of peptone, potassium dihydrogen phosphate
0.45~0.55 gram, 0.225~0.275 gram of magnesium sulfate, 0.9~1.1 milligram of thiamine, appropriate amount of water add to 250mL.
According to the fluid nutrient medium for being used for the Germination Strain that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Potato
10 grams, 10 grams of wheat bran, 10 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, thiamines
1 milligram of element, appropriate amount of water add to 250mL.
According to the fluid nutrient medium for being used for the Germination Strain that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Potato
25 grams, 6 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, 1 milligram of thiamine, water
Add to 250mL in right amount.
According to the fluid nutrient medium for being used for the Germination Strain that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Potato
25 grams, 6 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, 1 milligram of thiamine, wood
5 grams of bits, appropriate amount of water add to 250mL.
According to the fluid nutrient medium for being used for the Germination Strain that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Corn
10 grams of powder, 10 grams of wheat bran, 6.25 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate,
1 milligram of thiamine, appropriate amount of water add to 250mL.
According to the fluid nutrient medium for being used for the Germination Strain that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Corn
10 grams of powder, 10 grams of wheat bran, 6.25 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate,
1 milligram of thiamine, 5 grams of wood chip, appropriate amount of water add to 250mL.
According to the fluid nutrient medium for being used for the Germination Strain that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Potato
10 grams, 6 grams of wheat bran, 16 grams of corn flour, 5 grams of white sugar, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, sulphur
1 milligram of amine element, 5 grams of wood chip, appropriate amount of water add to 250mL.
According to the fluid nutrient medium for being used for the Germination Strain that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Potato
20 grams, 6 grams of wheat bran, 10 grams of corn flour, 5 grams of white sugar, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, 1 milligram of thiamine, water fits
Amount adds to 250mL.
According to the fluid nutrient medium for being used for the Germination Strain that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Potato
10 grams, 15 grams of corn flour, 6 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, sulphur
1 milligram of amine element, appropriate amount of water add to 250mL.
According to the fluid nutrient medium for being used for the Germination Strain that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Potato
10 grams, 15 grams of corn flour, 6 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, sulphur
1 milligram of amine element, 5 grams of wood chip, appropriate amount of water add to 250mL.
According to the fluid nutrient medium for being used for the Germination Strain that rhizoma Gastrodiae is planted of the present invention, wherein included in terms of every 250mL：Corn
16 grams of powder, 6 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, 1 milligram of thiamine,
5 grams of wood chip, appropriate amount of water add to 250mL.
According to the fluid nutrient medium for being used for the Germination Strain that rhizoma Gastrodiae is planted of the present invention, 2 grams are wherein also included in terms of every 250mL
Fructose and 0.02 gram of copper chloride.Had now surprisingly been found that, when in the fluid nutrient medium to Germination Strain simultaneously add above-mentioned amount
Fructose and copper chloride when, Mycelium growth rate can be significantly improved.
According to the fluid nutrient medium for being used for the Germination Strain that rhizoma Gastrodiae is planted of the present invention, it is to be prepared via a method which to obtain
's：Each solid material is weighed, adds appropriate amount of water (ordinarily be about 70~80% with liquid product), stirs, boil 20~30 points
Clock, add water to prescribed volume, continue to boil 10 minutes, be dispensed into conical flask, every bottle of 125mL, 5 glass are added in each bottle
Glass ball, is then sealed with cotton, then 121 degree of autoclavings 30 minutes in autoclave, is produced.
Further, third aspect present invention is related to rhizoma Gastrodiae implantation methods, and it is trained including the use of Germination Strain fluid nutrient medium
The step of supporting Germination Strain and using halimasch liquid medium culture halimasch.
Rhizoma Gastrodiae implantation methods according to a third aspect of the present invention, wherein the Germination Strain fluid nutrient medium, in terms of every 250mL
Wherein include：8~12 grams of potato, 8~12 grams of wheat bran, 8~12 grams of dregs of beans, 4~6 grams of white sugar, 0.5~1 gram of peptone, di(2-ethylhexyl)phosphate
0.4~0.6 gram of hydrogen potassium, 0.2~0.3 gram of magnesium sulfate, 0.8~1.2 milligram of thiamine, appropriate amount of water add to 250mL.
Rhizoma Gastrodiae implantation methods according to a third aspect of the present invention, wherein the Germination Strain fluid nutrient medium, in terms of every 250mL
Wherein include：9~11 grams of potato, 9~11 grams of wheat bran, 9~11 grams of dregs of beans, 4.5~5.5 grams of white sugar, peptone 0.65~0.85
Gram, 0.45~0.55 gram of potassium dihydrogen phosphate, 0.225~0.275 gram of magnesium sulfate, 0.9~1.1 milligram of thiamine, appropriate amount of water add to
250mL。
Rhizoma Gastrodiae implantation methods according to a third aspect of the present invention, wherein the Germination Strain fluid nutrient medium, in terms of every 250mL
Wherein include：10 grams of potato, 10 grams of wheat bran, 10 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, sulfuric acid
0.25 gram of magnesium, 1 milligram of thiamine, appropriate amount of water add to 250mL.
Rhizoma Gastrodiae implantation methods according to a third aspect of the present invention, wherein the Germination Strain fluid nutrient medium is in terms of every 250mL
Wherein also include 2 grams of fructose and 0.02 gram of copper chloride.
Rhizoma Gastrodiae implantation methods according to a third aspect of the present invention, wherein the Germination Strain fluid nutrient medium is by such as lower section
What method was prepared：Each solid material is weighed, adds appropriate amount of water (ordinarily be about 70~80% with liquid product), stirs, boil
Boiling 20~30 minutes, adds water to prescribed volume, continues to boil 10 minutes, be dispensed into conical flask, every bottle of 125mL, each bottle
5 glass marbles of middle addition, are then sealed with cotton, then 121 degree of autoclavings 30 minutes in autoclave, are produced.
Rhizoma Gastrodiae implantation methods according to a third aspect of the present invention, wherein the halimasch liquid culture medium, in terms of every 250mL
Wherein include：8~12 grams of corn flour, 8~12 grams of wheat bran, 5.5~7 grams of dregs of beans, 4~6 grams of white sugar, 0.5~1 gram of peptone, phosphorus
0.4~0.6 gram of acid dihydride potassium, 0.2~0.3 gram of magnesium sulfate, 0.8~1.2 milligram of thiamine, appropriate amount of water add to 250mL.
Rhizoma Gastrodiae implantation methods according to a third aspect of the present invention, wherein the halimasch liquid culture medium, in terms of every 250mL
Wherein include：9~11 grams of corn flour, 9~11 grams of wheat bran, 6~6.5 grams of dregs of beans, 4.5~5.5 grams of white sugar, peptone 0.65~
0.85 gram, 0.45~0.55 gram of potassium dihydrogen phosphate, 0.225~0.275 gram of magnesium sulfate, 0.9~1.1 milligram of thiamine, appropriate amount of water
Add to 250mL.
Rhizoma Gastrodiae implantation methods according to a third aspect of the present invention, wherein the halimasch liquid culture medium, in terms of every 250mL
Wherein include：10 grams of corn flour, 10 grams of wheat bran, 6.25 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate,
0.25 gram of magnesium sulfate, 1 milligram of thiamine, appropriate amount of water add to 250mL.
Rhizoma Gastrodiae implantation methods according to a third aspect of the present invention, wherein with every 250mL in the halimasch liquid culture medium
Meter wherein also includes 2.5 grams of maltose and 0.05 gram of copper chloride.
Rhizoma Gastrodiae implantation methods according to a third aspect of the present invention, wherein the halimasch liquid culture medium is by such as lower section
What method was prepared：Each solid material is weighed, adds appropriate amount of water (ordinarily be about 70~80% with liquid product), stirs, boil
Boiling 20~30 minutes, adds water to prescribed volume, continues to boil 10 minutes, be dispensed into conical flask, every bottle of 125mL, each bottle
5 glass marbles of middle addition, are then sealed with cotton, then 121 degree of autoclavings 30 minutes in autoclave, are produced.
Rhizoma Gastrodiae is orchid family, the saprophytic herbaceous plant of Gastrodia.Plant is up to 2 meters；Tuberosity, ellipse to nearly dumb-bell shape, meat
Matter, stem is upright, orange-yellow, yellow, taupe brown or blue-green, no greenery, raceme, petal length of a film round shape lanceolar, and flower is turned round
Turn, orange, yellowish, bluish-green or yellow-white, sepal and petal symphysis are into sepal is from raw ovate triangle, the capsule ellipse of falling ovate
Shape, the 5-7 months yield positive results.It is distributed in Jilin Province, China, Liaoning, the Inner Mongol, Hebei, Shanxi, Shaanxi, Gansu, Jiangsu, Anhui, Zhejiang
River, Jiangxi, Taiwan, Henan, Hubei, Hunan, Sichuan, Guizhou, Yunnan and Tibet.It is born under sparse woods, chearance, border, fills
Cong Bianyuan, Nepal, Bhutan, India, Japan, the Korea peninsula to Siberia are also distributed.Rhizoma Gastrodiae is one of rare traditional Chinese medicine.
Rhizoma Gastrodiae is used for medicinal purpose existing more than 1,000 years history, and the health preserving wine led to rhizoma Gastrodiae is considered as treasure in palace.Ancient medical book《Legendary god of farming's book on Chinese herbal medicine
Through》With《Compendium of Materia Medica》In, there is the record of rhizoma Gastrodiae " except all kinds of diseases and ailments prolong life ".
Rhizoma Gastrodiae root-like stock is plump, tuberosity, and ellipse is to nearly dumb-bell shape, and meat is long 8-12 centimetres, diameter 3-5 (7) li
Rice, sometimes bigger, the closeer section of tool, by the sheath of many triangular shape width egg shapes on section.Stem is upright, orange-yellow, yellow, taupe brown or
Blue-green, no greenery, bottom is by several pieces of film quality sheaths.The long 5-30 of raceme (50) centimetre of rhizoma Gastrodiae, generally have 30-50 flower；
Petal length of a film round shape lanceolar, long 1-1.5 centimetres, film quality；Bennet and the long 7-12 millimeters of ovary, slightly shorter than petal piece；Flower reverses,
Orange, yellowish, bluish-green or yellow-white, it is near upright；Sepal and petal symphysis into perianth tube be about 1 centimetre, diameter 5-7 millimeters,
Nearly tiltedly ovate cylindrical shape, top have 5 pieces of slivers, but the breach at front that is, two pieces of side sepal symphysis is as deep as 5 millimeters, the base of cylinder
Protrude forwards in portion；Foreign steamer sliver (sepal is from first portion) ovate triangle, tip are blunt；Lubrication groove sliver (petal is from first portion) is near
Long Circle is smaller；The long round shape oval of lip, long 6-7 millimeters, wide 3-4 millimeters, 3 split, and base portion adhesion is in stamen post foot end and flower
On tube inwall and there are a pair of meat corpus callosums, top there are irregular short tasselled from life, upper mask mastoid process, edge；Stamen column length 5-7
Millimeter, there is short stamen post foot.Capsule obovate elliptic, long 1.4-1.8 centimetres, wide 8-9 millimeters.The flowering fruit bearing stage 5-7 months.
Rhizoma Gastrodiae unrooted is without leaf, it is impossible to carries out photosynthesis, is by halimasch supply nutrient growth procreation.Thus plantation day
The first step of fiber crops is to cultivate a number of high-quality halimasch material, and second step, which is only, draws purchase Gastrodia.And in time and halimasch
Material is with cultivation.Environment：10-30 DEG C of the preference temperature of rhizoma Gastrodiae growth, 20-25 DEG C of optimum temperature, relative air humidity 80% or so, soil
Earth water content 50%-55%, pH value 5-6, i.e. slant acidity ecological environment.
Rhizoma Gastrodiae propagation method is it is well known that rhizoma Gastrodiae is mainly bred with stem tuber, it is also possible to seminal propagation.(1) stem tuber is numerous
Grow：Winter plants or spring planting.Winter plants rhizoma Gastrodiae and connects bacterium rate height, and growth is fast, and the time is in November.Spring planting is in the 3-4 months.Bacterium has been cultivated before cultivation
Bed.The seeds of suitable halimasch growth, with bark it is thick, it is wooden it is hard, wooden rough porosity is big, strong without aromatic substance, rotproofness
Broad leaf tree preferably.(2) seminal propagation：The arrow that selection weighs more than 100 grams is numb, and sunlight is prevented by upper method with adopting with cultivation, during bolting
Irradiation, will carry out artificial pollination when blooming.During the optional fine day 10 of pollination time or so, when anther cap lid edge is micro- now spend when carry out.
Fruit ear is entangled with polybag after pollination, when fruit has a small amount of seed to shed when bottom, from bottom to top with ripe with receipts.Due to Gastrodia
Sub- short life, the capsule under adopting answer sowing in time.
Gastrodin cultivation is generally April in late October to next year then.Broadcast before late October to early December frozen soil
Kind is planted for the winter, and it is spring planting that spring 3-4 lunar regolith is sowed after thawing, and the High aititude mountain area sowing time can be postponed until May.
Rhizoma Gastrodiae is rare Chinese medicine, to treat have a dizzy spell, the disease such as extremity numbness, child convulsion.Gastrodia Elata Stem Tuber：It is sweet, put down.
It is calming the liver to stop the wind, only convulsion.For dizziness of having a headache, extremity numbness, child convulsion, epilepsy clonus, lockjaw.【Jingpo medicine】：Control high blood
Pressure, dizziness, insomnia, headache, infantile convulsion《Dehong medicine is recorded》.【A'chang nationality medicine】Mao Zeer：The same Jingpo Nationality of function《Dehong medicine is recorded》.
【Deang medicine】Rhizoma Gastrodiae：The same Jingpo Nationality of function《Dehong medicine is recorded》.【Lisu medicine】Hang and mend door：Stem tuber controls dizziness pupil, head wind headache, limb
Body is numb, hemiplegia, aphasia, and pediatric epilepsy scared moves wind《Nujiang medicine》.【Baiyao】Rhizoma Gastrodiae, rhizoma gastrodiae, RHIZONA GASTRODIAE：Stem tuber controls height
Blood pressure, dizziness, headache etc.《Dali provides will》.【Hani medicine】Rhizoma Gastrodiae：Stem tuber controls stomach trouble《Hani medicine》.【Anaesthetic】Ulan-Suo Mo, east
Bu E, Suo Sile-Ga Er：Stem tuber, which is controlled, has a dizzy spell, extremity numbness, child convulsion, epilepsy, hypertension, aural vertigo《Cover
Medicine》.Noisy Hai Yin-good day loud, high-pitched sound：Hypertension is controlled, is had a headache, dizziness, apoplexy, facial paralysis, hemiplegia, child convulsion, frightened epilepsy takes out
Jerk, lockjaw, arthralgia pain due to rheumatism, extremity numbness《Cover and plant medicine will》.【Seedling medicine】Stem tuber is used to have a dizzy spell, and extremity numbness, children shy
Wind, epilepsy, high blood pressure, aural vertigo《Hunan indigo plant is examined》.Potato has, Gao Li：Rhizome controls dizziness, and stomachache, dizziness is intended to down, partially
It is aching all over the head《Seedling medicine》.The significant application value of rhizoma Gastrodiae clinically as can be seen here, opened hence for the planting technology of rhizoma Gastrodiae
Hair, has extremely important realistic meaning.The present invention is by the halimasch planted for rhizoma Gastrodiae and the Liquid Culture of Germination Strain
The optimization of base, have been obtained for improving the fluid nutrient medium of rhizoma Gastrodiae production efficiency.
Embodiment
The present invention can be further described by the following examples, however, the scope of the present invention and unlimited
In following embodiments.One of skill in the art, can be with it is understood that on the premise of without departing substantially from the spirit and scope of the present invention
Various change and modification are carried out to the present invention.The present invention carries out general to the material and test method that are arrived used in experiment
And/or specific description.Although for realize many materials used in the object of the invention and operating method be it is known in the art that
But the present invention is still described in detail as far as possible herein.Following examples further illustrate the present invention, rather than limit this hair
It is bright.
Embodiment 1：Halimasch and the screening of Germination Strain fluid nutrient medium
1st, experiment material
1.1st, equipment and material
Air-conditioning, back and forth formula shaking table, autoclave, conical flask, cotton, electric furnace, pot, electronic balance, the wild halimasch in Newcastle township,
Germination Strain (Shanxi Province's dendrobium mixture), superclean bench, inoculation spoon, alcolhol burner, glass marble, sieves, halimasch bacterium material culture
Base (self-control), Germination Strain leaf culture medium (self-control).
1.2nd, 1~formula of close ring bacterium culture medium formula 10, is respectively configured to 250mL with water：
Close 38 grams of 1. potato of ring bacterium culture medium formula, 2 grams of wheat bran, 5 grams of white sugar, 0.75 gram of peptone, potassium dihydrogen phosphate 0.5
Gram, 0.25 gram of magnesium sulfate, 1 milligram of thiamine；
Close 25 grams of 2. potato of ring bacterium culture medium formula, 12 grams of corn flour, 2.5 grams of white sugar, 0.75 gram of peptone, biphosphate
0.5 gram of potassium, 0.25 gram of magnesium sulfate, 1 milligram of thiamine；
25 grams of 3. potato of close ring bacterium culture medium formula, 6 grams of dregs of beans, 2.5 grams of white sugar, 2.5 grams of brown sugar, 0.75 gram of peptone,
0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, 1 milligram of thiamine；
25 grams of 4. potato of close ring bacterium culture medium formula, 11 grams of wheat bran, 3.7 grams of white sugar, 3.7 grams of brown sugar, 0.75 gram of peptone,
0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, 1 milligram of thiamine；
Close 7.5 grams of 5. dregs of beans of ring bacterium culture medium formula, 3.7 grams of wood chip, 2.5 grams of white sugar, 2.5 grams of brown sugar, peptone 0.75
Gram, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, 1 milligram of thiamine；
Close 10 grams of 6. wheat bran of ring bacterium culture medium formula, 10 grams of corn flour, 6.25 grams of dregs of beans, 5 grams of white sugar, peptone 0.75
Gram, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, 1 milligram of thiamine；
Close 30 grams of 7. potato of ring bacterium culture medium formula, 6 grams of wheat bran, 2.5 grams of white sugar, 5 grams of wood chip, 0.75 gram of peptone, phosphorus
0.5 gram of acid dihydride potassium, 0.25 gram of magnesium sulfate, 1 milligram of thiamine；
Close 25 grams of 8. potato of ring bacterium culture medium formula, 12 grams of wheat bran, 10 grams of corn flour, 5 grams of white sugar, 5 grams of wood chip, peptone
0.75 gram, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, 1 milligram of thiamine；
Close 25 grams of 9. potato of ring bacterium culture medium formula, 6 grams of dregs of beans, 2.5 grams of white sugar, 2.5 grams of brown sugar, 10 grams of wood chip, albumen
0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, 1 milligram of thiamine；
Close 12 grams of 10. wheat bran of ring bacterium culture medium formula, 10 grams of dregs of beans, 5 grams of wood chip, 2.5 grams of white sugar, 2.5 grams of brown sugar, albumen
0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, 1 milligram of thiamine.
1.3rd, Germination Strain culture medium prescription 1~formula 10, is respectively configured to 250mL with water：
25 grams of 1. potato of Germination Strain culture medium prescription, 6 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, potassium dihydrogen phosphate 0.5
Gram, 0.25 gram of magnesium sulfate, 1 milligram of thiamine；
25 grams of 2. potato of Germination Strain culture medium prescription, 6 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, potassium dihydrogen phosphate 0.5
Gram, 0.25 gram of magnesium sulfate, 1 milligram of thiamine, 5 grams of wood chip；
10 grams of 3. corn flour of Germination Strain culture medium prescription, 10 grams of wheat bran, 6.25 grams of dregs of beans, 5 grams of white sugar, peptone 0.75
Gram, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, 1 milligram of thiamine；
10 grams of 4. corn flour of Germination Strain culture medium prescription, 10 grams of wheat bran, 6.25 grams of dregs of beans, 5 grams of white sugar, peptone 0.75
Gram, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, 1 milligram of thiamine, 5 grams of wood chip；
10 grams of 5. potato of Germination Strain culture medium prescription, 6 grams of wheat bran, 16 grams of corn flour, 5 grams of white sugar, 0.75 gram of peptone, phosphorus
0.5 gram of acid dihydride potassium, 0.25 gram of magnesium sulfate, 1 milligram of thiamine, 5 grams of wood chip；
10 grams of 6. potato of Germination Strain culture medium prescription, 10 grams of wheat bran, 10 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, phosphorus
0.5 gram of acid dihydride potassium, 0.25 gram of magnesium sulfate, 1 milligram of thiamine；
20 grams of 7. potato of Germination Strain culture medium prescription, 6 grams of wheat bran, 10 grams of corn flour, 5 grams of white sugar, potassium dihydrogen phosphate 0.5
Gram, 0.25 gram of magnesium sulfate, 1 milligram of thiamine；
10 grams of 8. potato of Germination Strain culture medium prescription, 15 grams of corn flour, 6 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, phosphorus
0.5 gram of acid dihydride potassium, 0.25 gram of magnesium sulfate, 1 milligram of thiamine；
10 grams of 9. potato of Germination Strain culture medium prescription, 15 grams of corn flour, 6 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, phosphorus
0.5 gram of acid dihydride potassium, 0.25 gram of magnesium sulfate, 1 milligram of thiamine, 5 grams of wood chip；
16 grams of 10. corn flour of Germination Strain culture medium prescription, 6 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, potassium dihydrogen phosphate
0.5 gram, 0.25 gram of magnesium sulfate, 1 milligram of thiamine, 5 grams of wood chip.
2nd, method
2.1st, strain pre-processes：
Treat that spawn activity is extensive, it is necessary to be put into room temperature condition and transfer 2-3 days if being placed on the strain preserved under cryogenic conditions
It could be inoculated with after multiple；It need not activate and directly can be inoculated into fluid nutrient medium in the strain of culturing room's culture.
2.2nd, culture medium is prepared：
Each solid material is weighed according to culture medium prescription, adds appropriate amount of water (ordinarily be about 70~80% with liquid product), stirs
Mix uniformly, boil 20~30 minutes, add water to prescribed volume, continue to boil 10 minutes, be dispensed into conical flask, every bottle
125mL, 5 glass marbles are added in each bottle, are then sealed with cotton, then 121 degrees Celsius of autoclavings 30 divide in autoclave
Clock, produce.
2.3rd, it is inoculated with and cultivates：
Halimasch and Germination Strain are inoculated into halimasch liquid culture medium and Germination Strain fluid nutrient medium respectively, every bottle connects
Enter 1 cubic centimetre and strain block try one's best it is small.Lucifuge culture in shaking table is put into after inoculation is good, halimasch shaking speed is 143
Turn/min, Germination Strain shaking speed is 155 turns/min, and cultivation temperature airconditioning control is at 16~24 degrees Celsius.
After culture medium becomes clear, halimasch and Germination Strain liquid strain are transferred to halimasch bacterium material culture medium and sprouting respectively
In bacterium leaf culture medium, the culture of lucifuge culturing room is then put into, control temperature observes halimasch and sprouting at 16~25 degrees Celsius
The restoration ecosystem situation of bacterium.
It is well known that for strain plantation, the liquid spawn major requirement of culture is：Bacterium ball is uniform, and will
As far as possible small, Peloton density is moderate, and Peloton density is just in 80%-90% preferably when culture medium is clarified, if bacterium ball is close
Spend Gao Zehui has harmful effect to subsequent operation；Incubation time is as far as possible short；It is extensive when liquid spawn is transferred in solid medium
Multiple growth adaptability is eager to excel.
3rd, result：
3.1st, halimasch cultivated in different culture media growth different time, from bacterium bulb diameter (mm), strain bulk density,
Investigation/evaluation in terms of bacterium ball suspending power, bacterium ball circularity, culture medium clarification, is as a result shown in Table 1, table 2 and table 3 respectively.
Table 1：The influence (culture 3 days) that different culture media grows to halimasch
In table 1, and in the present invention, two parameters of bacterium ball suspending power and bacterium ball circularity are given a mark by subjectivity in a manner of evaluation,
With 0-10 points for standards of grading, 0 point worst, and 10 points best, i.e., score value is the higher the better.
Table 2：The influence (culture 5 days) that different culture media grows to halimasch
| Culture medium | Bacterium bulb diameter (mm) | Strain bulk density | Bacterium ball suspending power | Bacterium ball circularity | Culture medium clarity |
| 1 | 1-3 | 12% | 1 | 4 | It is muddy |
| 2 | 1-5 | 34% | 4 | 3 | It is muddy |
| 3 | 1-3 | 42% | 2 | 3 | It is muddy |
| 4 | 1-4 | 43% | 4 | 4 | It is muddy |
| 5 | 1-3 | 45% | 4 | 5 | It is muddy |
| 6 | 1-3 | 87% | 10 | 10 | Clarification |
| 7 | 1-3 | 19% | 2 | 3 | It is muddy |
| 8 | 1-5 | 47% | 5 | 5 | It is muddy |
| 9 | 1-3 | 68% | 7 | 5 | It is muddy |
| 10 | 1-3 | 65% | 6 | 6 | It is muddy |
Table 3：The influence (culture 7 days) that different culture media grows to halimasch
3.2nd, the halimasch of different culture media culture is in bacterium material culture basal growth, by different time, its upgrowth situation
Investigation/evaluation in terms of mycelia restoration ecosystem situation and the long artificial situation two of shoestring, is as a result shown in Table 4, table 5 and table 6 respectively.
Table 4：Upgrowth situation (culture 12 hours) in bacterium material culture medium of the halimasch of different culture media culture
| The strain of different culture media culture | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| Mycelia restoration ecosystem situation | 0 | 2 | 1 | 3 | 0 | 7 | 1 | 3 | 2 | 0 |
| The long artificial situation of shoestring | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 |
In table 4, and in the present invention, mycelia restoration ecosystem situation and two parameters of the long artificial situation of shoestring are commented with subjectivity marking
The mode of valency, with 0-10 points for standards of grading, 0 point worst, and 10 points best, i.e., score value is the higher the better.
Table 5：Upgrowth situation (culture 3 days) in bacterium material culture medium of the halimasch of different culture media culture
| The strain of different culture media culture | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| Mycelia restoration ecosystem situation | 3 | 4 | 2 | 5 | 2 | 10 | 3 | 5 | 4 | 3 |
| The long artificial situation of shoestring | 0 | 0 | 0 | 3 | 0 | 8 | 1 | 4 | 2 | 3 |
Table 6：Upgrowth situation (culture 6 days) in bacterium material culture medium of the halimasch of different culture media culture
| The strain of different culture media culture | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| Mycelia restoration ecosystem situation | 5 | 6 | 4 | 6 | 4 | 10 | 5 | 6 | 6 | 4 |
| The long artificial situation of shoestring | 1 | 2 | 1 | 5 | 1 | 10 | 2 | 5 | 2 | 4 |
According to the result of 1~table of upper table 6, it can be seen that No. 6 fluid nutrient medium incubation times of halimasch are most short, bacterium ball is big
The small and medium density and most strong mycelia of adaptability recovers when being transferred in planted medium and shoestring is grown soon, therefore halimasch
No. 6 fluid nutrient mediums are more suitably applied to rhizoma Gastrodiae plantation culture halimasch liquid bacterial strains.
3.3rd, Germination Strain cultivated in different culture media growth different time, from bacterium bulb diameter (mm), strain bulk density,
Investigation/evaluation in terms of bacterium ball suspending power, bacterium ball circularity, culture medium clarification, is as a result shown in Table 7, table 8 and table 9 respectively.
Table 7：Influence (culture 3 day) of the different culture media to sprouting bacteria growing
| Culture medium | Bacterium bulb diameter (mm) | Strain bulk density | Bacterium ball suspending power | Bacterium ball circularity | Culture medium clarity |
| 1 | 1-2 | 3% | 2 | 3 | It is muddy |
| 2 | 1-2 | 2% | 3 | 3 | It is muddy |
| 3 | 1-3 | 5% | 3 | 1 | It is muddy |
| 4 | 1-3 | 4% | 2 | 2 | It is muddy |
| 5 | 1-4 | 10% | 1 | 3 | It is muddy |
| 6 | 1-2 | 7% | 7 | 8 | It is muddy |
| 7 | 1-4 | 6% | 2 | 4 | It is muddy |
| 8 | 1-4 | 3% | 1 | 0 | It is muddy |
| 9 | 1-4 | 2% | 2 | 0 | It is muddy |
| 10 | 1-4 | 3% | 2 | 1 | It is muddy |
Table 8：Influence (culture 7 day) of the different culture media to sprouting bacteria growing
| Culture medium | Bacterium bulb diameter (mm) | Strain bulk density | Bacterium ball suspending power | Bacterium ball circularity | Culture medium clarity |
| 1 | 2-3 | 75% | 3 | 3 | Clarification |
| 2 | 2-3 | 73% | 4 | 5 | Clarification |
| 3 | 2-3 | 82% | 4 | 3 | Clarification |
| 4 | 4-8 | 78% | 3 | 4 | Clarification |
| 5 | 4-8 | 87% | 2 | 4 | Clarification |
| 6 | 3-4 | 86% | 10 | 10 | Clarification |
| 7 | 4-10 | 46% | 3 | 5 | It is muddy |
| 8 | 5-12 | 48% | 2 | 1 | It is muddy |
| 9 | 5-12 | 44% | 3 | 2 | It is muddy |
| 10 | 5-12 | 47% | 3 | 1 | It is muddy |
Table 9：Influence (culture 10 day) of the different culture media to sprouting bacteria growing
| Culture medium | Bacterium bulb diameter (mm) | Strain bulk density | Bacterium ball suspending power | Bacterium ball circularity | Culture medium clarity |
| 1 | 2-4 | 77% | 5 | 4 | Clarification |
| 2 | 2-4 | 75% | 5 | 6 | Clarification |
| 3 | 2-4 | 84% | 5 | 5 | Clarification |
| 4 | 4-9 | 79% | 5 | 6 | Clarification |
| 5 | 4-8 | 91% | 6 | 6 | Clarification |
| 6 | 3-4 | 88% | 10 | 10 | Clarification |
| 7 | 5-16 | 62% | 6 | 7 | Clarification |
| 8 | 5-14 | 59% | 4 | 3 | Clarification |
| 9 | 5-17 | 65% | 5 | 3 | Clarification |
| 10 | 5-16 | 67% | 4 | 3 | Clarification |
3.4th, the Germination Strain of different culture media culture is in leaf culture basal growth, by different time, its upgrowth situation
Investigation/evaluation in terms of mycelia restoration ecosystem situation, is as a result shown in Table 10, table 11 respectively.
Table 10：Upgrowth situation (culture 12 hours) in leaf culture medium of the Germination Strain of different culture media culture
| The strain of different culture media culture | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| Mycelia restoration ecosystem situation | 1 | 0 | 1 | 3 | 2 | 7 | 1 | 2 | 0 | 1 |
Table 11：Upgrowth situation (culture 3 days) in leaf culture medium of the Germination Strain of different culture media culture
| The strain of different culture media culture | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| Mycelia restoration ecosystem situation | 2 | 1 | 3 | 5 | 4 | 9 | 3 | 4 | 1 | 3 |
Find out short No. 6 fluid nutrient medium incubation times of Germination Strain, bacterium ball size and close from that can be integrated with 7~table of upper table 11
Spend best and to be transferred to strong adaptability mycelia restoration ecosystem when in Germination Strain planted medium good, therefore No. 6 liquid of Germination Strain
Body culture medium is best suitable for being applied to rhizoma Gastrodiae plantation culture Germination Strain liquid spawn.
Embodiment 2：Halimasch and the screening of Germination Strain fluid nutrient medium
Reference implementation example 1, difference is, is only carried out with close ring bacterium culture medium formula 6 and Germination Strain culture medium prescription 6
Experiment, still
Close ring bacterium culture medium formula 6 therein is changed to as follows：Wherein comprising 12 grams of corn flour, wheat bran 8 in terms of every 250mL
Gram, 5.5 grams of dregs of beans, 6 grams of white sugar, 0.5 gram of peptone, 0.6 gram of potassium dihydrogen phosphate, 0.2 gram of magnesium sulfate, 1.2 milligrams of thiamine, water
Add to 250mL in right amount；And Germination Strain culture medium prescription 6 therein is changed to as follows：In terms of every 250mL wherein comprising 8 grams of potato,
12 grams of wheat bran, 8 grams of dregs of beans, 6 grams of white sugar, 0.5 gram of peptone, 0.4 gram of potassium dihydrogen phosphate, 0.3 gram of magnesium sulfate, the milli of thiamine 0.8
Gram, appropriate amount of water add to 250mL.
As a result show, the knot for the result each table corresponding to embodiment 1 presented with 1~table of table, 11 each table that the present embodiment obtains
Fruit is essentially identical, no significant difference.
Embodiment 3：Halimasch and the screening of Germination Strain fluid nutrient medium
Reference implementation example 1, difference is, is only carried out with close ring bacterium culture medium formula 6 and Germination Strain culture medium prescription 6
Experiment, still
Close ring bacterium culture medium formula 6 therein is changed to as follows：Wherein comprising 8 grams of corn flour, wheat bran 12 in terms of every 250mL
Gram, 7 grams of dregs of beans, 4 grams of white sugar, 1 gram of peptone, 0.4 gram of potassium dihydrogen phosphate, 0.3 gram of magnesium sulfate, 0.8 milligram of thiamine, appropriate amount of water
Add to 250mL；And Germination Strain culture medium prescription 6 therein is changed to as follows：Wherein comprising 12 grams of potato, bran in terms of every 250mL
8 grams of skin, 12 grams of dregs of beans, 4 grams of white sugar, 1 gram of peptone, 0.6 gram of potassium dihydrogen phosphate, 0.2 gram of magnesium sulfate, 1.2 milligrams of thiamine, water
Add to 250mL in right amount.
As a result show, the knot for the result each table corresponding to embodiment 1 presented with 1~table of table, 11 each table that the present embodiment obtains
Fruit is essentially identical, no significant difference.
Embodiment 4：Halimasch and the screening of Germination Strain fluid nutrient medium
Reference implementation example 1, difference is, is only carried out with close ring bacterium culture medium formula 6 and Germination Strain culture medium prescription 6
Experiment, still
Close ring bacterium culture medium formula 6 therein is changed to as follows：Wherein comprising 9 grams of corn flour, wheat bran 11 in terms of every 250mL
Gram, 6 grams of dregs of beans, 4.5 grams of white sugar, 0.85 gram of peptone, 0.45 gram of potassium dihydrogen phosphate, 0.225 gram of magnesium sulfate, the milli of thiamine 0.9
Gram, appropriate amount of water add to 250mL；And Germination Strain culture medium prescription 6 therein is changed to as follows：Wherein comprising soil in terms of every 250mL
11 grams of beans, 11 grams of wheat bran, 9 grams of dregs of beans, 4.5 grams of white sugar, 0.85 gram of peptone, 0.45 gram of potassium dihydrogen phosphate, magnesium sulfate 0.275
Gram, 0.9 milligram of thiamine, appropriate amount of water add to 250mL.
As a result show, the knot for the result each table corresponding to embodiment 1 presented with 1~table of table, 11 each table that the present embodiment obtains
Fruit is essentially identical, no significant difference.
Embodiment 5：Halimasch and the screening of Germination Strain fluid nutrient medium
Reference implementation example 1, difference is, is only carried out with close ring bacterium culture medium formula 6 and Germination Strain culture medium prescription 6
Experiment, still
Close ring bacterium culture medium formula 6 therein is changed to as follows：Wherein comprising 11 grams of corn flour, wheat bran 9 in terms of every 250mL
Gram, 6.5 grams of dregs of beans, 5.5 grams of white sugar, 0.65 gram of peptone, 0.55 gram of potassium dihydrogen phosphate, 0.275 gram of magnesium sulfate, thiamine 1.1
Milligram, appropriate amount of water add to 250mL；And Germination Strain culture medium prescription 6 therein is changed to as follows：Wherein included in terms of every 250mL
9 grams of potato, 9 grams of wheat bran, 11 grams of dregs of beans, 5.5 grams of white sugar, 0.65 gram of peptone, 0.55 gram of potassium dihydrogen phosphate, magnesium sulfate 0.225
Gram, 1.1 milligrams of thiamine, appropriate amount of water add to 250mL.
As a result show, the knot for the result each table corresponding to embodiment 1 presented with 1~table of table, 11 each table that the present embodiment obtains
Fruit is essentially identical, no significant difference.
Embodiment 6：The hypha biomass measure of halimasch
Determine hypha biomass：After fermentation ends (that is, halimasch is cultivated 7 days), vacuum filtration collects mycelium, warm water punching
Remove culture medium of the attachment removal on shoestring and [Liu Bing, Niu Yun, Zhang Dezhu, wait rhizoma Gastrodiaes symbiosis halimasch parent species and liquid strain culture
Optimization [J] edible fungi of china of base, 2012,31 (3):28-31], 65 DEG C are dried to permanent quality, measurement mycelium dry mass (g/
100mL).After measured, the mycelium dry mass of No. 6 halimasch culture mediums is 0.44g/100mL in embodiment 1, remaining 9 kinds of sweet ring
The mycelium dry mass of bacterium culture medium has no obvious in the range of 0.24~0.42g/100mL between different culture media group
Difference；In addition, used in embodiment 2- embodiments 5 the mycelium dry mass of similar No. 6 halimasch culture mediums 0.42~
In the range of 0.45g/100mL and mutual indifference.In general, if mycelium dry mass can exceed that 0.5g/100mL
Think the excellent performance of this culture medium, and mycelium dry mass then thinks this culture in the range of 0.4~0.5g/100mL
Base can meet general use demand.In carried out complementary testing is operated with reference to 1~embodiment of the present embodiment 5, to No. 6 honey
Supplement 2.5 grams of maltose of addition and 0.05 gram of copper chloride (in terms of every 250mL), as a result find except mycelium in ring bacterium culture medium
Dry mass can significantly improve outer (mycelium dry mass can reach in the range of 0.68~0.74g/100mL), to other indexs without
It is obvious to influence；And work as in the complementary testing with reference to embodiment 1 to other 9 kinds of cultures in addition to No. 6 halimasch culture mediums
When above two material is added in base, mycelium dry mass can be significantly improved by having no, not less than 0.48g/100ml.But
In the experiment of supplement, in the complementary testing carried out with reference to 1~embodiment of above-described embodiment 5, if only added in culture medium
Maltose only adds copper chloride for the moment, can not improve No. 6 halimasch culture of mycelium dry mass such as embodiment 1
Above-mentioned amount maltose is only added in base and the culture medium of 2~embodiment of embodiment 5 or only adds copper chloride for the moment, mycelia
Soma quality is in the range of 0.42~0.46g/100mL.It can be seen that it is above-mentioned to supplement addition simultaneously in halimasch liquid culture medium
Mycelium dry mass is remarkably improved after two kinds of materials.
Embodiment 7：The investigation of mycelial growth rate
Above example 1 to the Germination Strain leaf culture medium as used in of embodiment 5 is own product, and it is classical Germination Strain tree
Leaf culture medium, matches and is：Fagaceae leaf, hardwood sawdust and wheat bran respectively account for 60%, 30% and 10%.Such as embodiment 1
The method of its " 2.3, inoculation and culture " second segment, loads quantitative Germination Strain in 17cm × 33cm polypropylene edible mushroom bag
Leaf culture medium, after sterilizing cooling, every bag is inoculated with 20mL Germination Strain liquid spawns, and marking pen is used in polypropylene after 25 DEG C of culture 7d
The growth line of mycelia is marked on edible mushroom bag, and as baseline, mycelial growth rate (cm/ days) is recorded after one month, is often located
Reason sets 5 repetitions.It has been found that 10 kinds of embodiment 1 are sprouted bacterium solution obtained by bacterium culture medium and carry out mycelial growth after above-mentioned inoculation
For speed in the range of 0.33~0.41cm/ days, embodiment 2 to the gained bacterium solution of embodiment 5 carries out mycelial growth after above-mentioned inoculation
Speed also in the range of 0.34~0.40cm/ days, shows the general still acceptable speed of growth.But in the experiment of supplement
In, when in embodiment 1- embodiments 5 when supplement addition 2 grams of fructose and 0.02 gram of chlorine in Germination Strain fluid nutrient medium thereto
When changing copper, Mycelium growth rate dramatically increases, particularly embodiment 1 No. 6 Germination Strain fluid nutrient medium and embodiment 2-
Bacterium solution mycelial growth rate after Germination Strain leaf culture medium is inoculated into obtained by the Germination Strain fluid nutrient medium culture of embodiment 5 is reachable
To in the range of 0.68~0.72cm/ days, this speed of growth is amazing.But in the experiment of supplement, with reference to upper
State in the complementary testing that 1~embodiment of embodiment 5 is carried out, if only adding fructose or only adding in Germination Strain fluid nutrient medium
Add copper chloride can not improve mycelial growth rate, such as No. 6 halimasch culture mediums and embodiment 2 of embodiment 1 for the moment
Above-mentioned amount fructose is only added in the culture medium of~embodiment 5 or only adds copper chloride for the moment, mycelial growth rate exists
In the range of 0.36~0.41cm/ days.It can be seen that it can show after supplementing addition above two material simultaneously in Germination Strain fluid nutrient medium
Write and improve mycelial growth rate.It should also be noted that, mycelial growth rate reflection is Germination Strain in Germination Strain leaf culture
The speed of growth in base, and above two material is in addition liquid medium within, to Germination Strain in the two liquid medium within
Effect extend in leaf culture medium and influence the effect of mycelial growth rate, be that the present inventor is still unaccountable so far.
In addition, although fructose and both maltose used in embodiment 6 that the present embodiment 7 uses are both carbohydrate, but when in the He of embodiment 6
Both sugar are exchanged in embodiment 7 in use, can not but obtain above-mentioned raising mycelial growth rate completely and improve mycelia soma
The effect of quality.
Embodiment described above is only to absolutely prove preferred embodiment that is of the invention and being lifted, protection model of the invention
Enclose not limited to this.The equivalent substitute or conversion that those skilled in the art are made on the basis of the present invention, in the present invention
Protection domain within.Protection scope of the present invention is defined by claims.
Claims (10)
1. the fluid nutrient medium of the Germination Strain for rhizoma Gastrodiae plantation, is wherein included in terms of every 250mL：8~12 grams of potato, wheat bran 8
~12 grams, 8~12 grams of dregs of beans, 4~6 grams of white sugar, 0.5~1 gram of peptone, 0.4~0.6 gram of potassium dihydrogen phosphate, magnesium sulfate 0.2~
0.3 gram, 0.8~1.2 milligram of thiamine, appropriate amount of water add to 250mL.
2. fluid nutrient medium according to claim 1, wherein included in terms of every 250mL：9~11 grams of potato, 9~11 grams of wheat bran,
9~11 grams of dregs of beans, 4.5~5.5 grams of white sugar, 0.65~0.85 gram of peptone, 0.45~0.55 gram of potassium dihydrogen phosphate, magnesium sulfate
0.225~0.275 gram, 0.9~1.1 milligram of thiamine, appropriate amount of water add to 250mL.
3. fluid nutrient medium according to claim 1, wherein included in terms of every 250mL：10 grams of potato, 10 grams of wheat bran, dregs of beans 10
Gram, 5 grams of white sugar, 0.75 gram of peptone, 0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, 1 milligram of thiamine, appropriate amount of water add to
250mL。
4. fluid nutrient medium according to claim 1, wherein included in terms of every 250mL：8 grams of potato, 12 grams of wheat bran, 8 grams of dregs of beans,
6 grams of white sugar, 0.5 gram of peptone, 0.4 gram of potassium dihydrogen phosphate, 0.3 gram of magnesium sulfate, 0.8 milligram of thiamine, appropriate amount of water add to
250mL。
5. fluid nutrient medium according to claim 1, wherein included in terms of every 250mL：12 grams of potato, 8 grams of wheat bran, dregs of beans 12
Gram, 4 grams of white sugar, 1 gram of peptone, 0.6 gram of potassium dihydrogen phosphate, 0.2 gram of magnesium sulfate, 1.2 milligrams of thiamine, appropriate amount of water add to
250mL。
6. fluid nutrient medium according to claim 1, wherein included in terms of every 250mL：11 grams of potato, 11 grams of wheat bran, dregs of beans 9
Gram, 4.5 grams of white sugar, 0.85 gram of peptone, 0.45 gram of potassium dihydrogen phosphate, 0.275 gram of magnesium sulfate, 0.9 milligram of thiamine, appropriate amount of water
Add to 250mL.
7. fluid nutrient medium according to claim 1, wherein included in terms of every 250mL：9 grams of potato, 9 grams of wheat bran, 11 grams of dregs of beans,
5.5 grams of white sugar, 0.65 gram of peptone, 0.55 gram of potassium dihydrogen phosphate, 0.225 gram of magnesium sulfate, 1.1 milligrams of thiamine, appropriate amount of water add
To 250mL.
8. fluid nutrient medium according to claim 1, it is characterised in that：
Wherein included in terms of every 250mL：25 grams of potato, 6 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, potassium dihydrogen phosphate 0.5
Gram, 0.25 gram of magnesium sulfate, 1 milligram of thiamine, appropriate amount of water add to 250mL；Or
Wherein included in terms of every 250mL：25 grams of potato, 6 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, potassium dihydrogen phosphate 0.5
Gram, 0.25 gram of magnesium sulfate, 1 milligram of thiamine, 5 grams of wood chip, appropriate amount of water add to 250mL；Or
Wherein included in terms of every 250mL：10 grams of corn flour, 10 grams of wheat bran, 6.25 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone,
0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, 1 milligram of thiamine, appropriate amount of water add to 250mL；Or
Wherein included in terms of every 250mL：10 grams of corn flour, 10 grams of wheat bran, 6.25 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone,
0.5 gram of potassium dihydrogen phosphate, 0.25 gram of magnesium sulfate, 1 milligram of thiamine, 5 grams of wood chip, appropriate amount of water add to 250mL；Or
Wherein included in terms of every 250mL：10 grams of potato, 6 grams of wheat bran, 16 grams of corn flour, 5 grams of white sugar, 0.75 gram of peptone, phosphoric acid
0.5 gram of potassium dihydrogen, 0.25 gram of magnesium sulfate, 1 milligram of thiamine, 5 grams of wood chip, appropriate amount of water add to 250mL；Or
Wherein included in terms of every 250mL：20 grams of potato, 6 grams of wheat bran, 10 grams of corn flour, 5 grams of white sugar, 0.5 gram of potassium dihydrogen phosphate,
0.25 gram of magnesium sulfate, 1 milligram of thiamine, appropriate amount of water add to 250mL；Or
Wherein included in terms of every 250mL：10 grams of potato, 15 grams of corn flour, 6 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, phosphoric acid
0.5 gram of potassium dihydrogen, 0.25 gram of magnesium sulfate, 1 milligram of thiamine, appropriate amount of water add to 250mL；Or
Wherein included in terms of every 250mL：10 grams of potato, 15 grams of corn flour, 6 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, phosphoric acid
0.5 gram of potassium dihydrogen, 0.25 gram of magnesium sulfate, 1 milligram of thiamine, 5 grams of wood chip, appropriate amount of water add to 250mL；Or
Wherein included in terms of every 250mL：16 grams of corn flour, 6 grams of dregs of beans, 5 grams of white sugar, 0.75 gram of peptone, potassium dihydrogen phosphate 0.5
Gram, 0.25 gram of magnesium sulfate, 1 milligram of thiamine, 5 grams of wood chip, appropriate amount of water add to 250m；Or
2 grams of fructose and 0.02 gram of copper chloride are wherein also included in terms of every 250mL.
9. fluid nutrient medium according to claim 1, it is prepared via a method which to obtain：Each solid material is weighed, is added
Appropriate amount of water, stir, boil 20~30 minutes, add water to prescribed volume, continue to boil 10 minutes, be dispensed into conical flask
In, every bottle of 125mL, 5 glass marbles are added in each bottle, are then sealed with cotton, then 121 degree of autoclavings 30 in autoclave
Minute, produce.
10. rhizoma Gastrodiae implantation methods, it is trained including the use of Germination Strain fluid nutrient medium culture Germination Strain and using halimasch liquid
The step of supporting base culture halimasch.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112608852A (en) * | 2021-01-06 | 2021-04-06 | 袁隆平农业高科技股份有限公司 | Inoculation and propagation method of fusarium verticillioides |
| CN114342958A (en) * | 2021-12-31 | 2022-04-15 | 贵州省园艺研究所(贵州省园艺工程技术研究中心) | Seed dressing microbial inoculum for promoting germination of orchid seeds and seed dressing and culturing method |
| CN114456954A (en) * | 2022-02-26 | 2022-05-10 | 西峡县占领菌业有限公司 | Liquid germination bacterium and preparation method thereof |
| CN116694481A (en) * | 2023-06-06 | 2023-09-05 | 陕西理工大学 | Detoxification screening method for gastrodia elata germination bacteria |
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2017
- 2017-10-20 CN CN201710981881.3A patent/CN107629967B/en active Active
Non-Patent Citations (1)
| Title |
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| 杨儒钦: "天麻蜜环菌、萌发菌母种生产技术", 《药用植物》 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112608852A (en) * | 2021-01-06 | 2021-04-06 | 袁隆平农业高科技股份有限公司 | Inoculation and propagation method of fusarium verticillioides |
| CN112608852B (en) * | 2021-01-06 | 2023-06-09 | 袁隆平农业高科技股份有限公司 | Fusarium verticillium inoculation and propagation method |
| CN114342958A (en) * | 2021-12-31 | 2022-04-15 | 贵州省园艺研究所(贵州省园艺工程技术研究中心) | Seed dressing microbial inoculum for promoting germination of orchid seeds and seed dressing and culturing method |
| CN114456954A (en) * | 2022-02-26 | 2022-05-10 | 西峡县占领菌业有限公司 | Liquid germination bacterium and preparation method thereof |
| CN116694481A (en) * | 2023-06-06 | 2023-09-05 | 陕西理工大学 | Detoxification screening method for gastrodia elata germination bacteria |
| CN116694481B (en) * | 2023-06-06 | 2024-04-16 | 陕西森盛菌业科技有限公司 | Detoxification screening method for gastrodia elata germination bacteria |
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