CN110278827A - A kind of method of Pleurotus nebrodensis field child care fruiting on the spot - Google Patents
A kind of method of Pleurotus nebrodensis field child care fruiting on the spot Download PDFInfo
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- CN110278827A CN110278827A CN201910619003.6A CN201910619003A CN110278827A CN 110278827 A CN110278827 A CN 110278827A CN 201910619003 A CN201910619003 A CN 201910619003A CN 110278827 A CN110278827 A CN 110278827A
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- pleurotus nebrodensis
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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Abstract
The invention discloses a kind of methods of Pleurotus nebrodensis field child care fruiting on the spot, preparation solid spawn is activated by using Pleurotus nebrodensis strain, by Pleurotus nebrodensis solid spawn field inoculation in asafoetide plant root, establish the technology of Pleurotus nebrodensis solid spawn field inoculation asafoetide plant, and realize field planting and fruiting of the Pleurotus ferulae mycelium in asafoetide plant roots base portion tissue, averagely field planting rate is up to 88.29%, average fructification incidence is up to 28.4%, it makes important progress on Pleurotus nebrodensis germplasm field on the spot child care technology, important technology guidance is provided for the child care on the spot and its ecological protection of Pleurotus nebrodensis germ plasm resource, Pleurotus nebrodensis germ plasm resource is set to protect and develop, there is wide applicability in terms of the protection of Pleurotus nebrodensis germ plasm resource.
Description
Technical field
The present invention relates to edible mushroom germ plasm resource child care technical fields on the spot, specifically, the present invention relates to a kind of Pleurotus nebrodensis
The method and technology field of field child care fruiting on the spot.
Background technique
Pleurotus nebrodensis, Chinese rectification of name Pleurotus nebrodensis (Pleurotus tuoliensis), be it is a kind of food, medical value it is all very high
Rare delicious edible mushroom." the The famous cultivated mushroom that Mengran Zhao in 2016 et al. is delivered
Bailinggu is a separate species of the Pleurotus eryngii species complex " article
It wore in " fungus journal " to kindly help secure the success of et al. with 2018 and deliver in " Chinese five kinds of important edible mushroom scientific names are newly infused " article, pass through and be
Both of Pleurotus ferulae Lanzi and Pleurotus nebrodensis fungies are distinguished in system development research, independent for 2 in systematic growth
Branch, and supporting rate is very high, and in addition, 2 kinds of distributed areas are also different, therefore Pleurotus nebrodensis is promoted to one independent kind.
In China, it is domestic that wild Pleurotus nebrodensis is only distributed in Xinjiang, is distributed mainly on the Resina Ferulae beach of Zhungeer Basin Edge gobi, and select
Property foundation portion tissue that is saprophytic or colonizing in Chinese medicine asafoetide plant, it is annual only in the first tenday period of a month fruiting in late April to May, distribution
It measures very rare.In recent years, since mankind's activity increases, the deterioration of the ecological environment, climate change, and resource is excessively excavated,
Wild Pleurotus nebrodensis resource ownership sharply declines, and the wild Pleurotus nebrodensis in some regions almost disappears.Therefore, Pleurotus nebrodensis resource is just
Ground child care and protection technique are urgently to be resolved.Currently, the research about Pleurotus nebrodensis germ plasm resource field child care technology has not been reported,
And Pleurotus nebrodensis solid spawn field inoculation asafoetide plant technology is established, realize Pleurotus ferulae mycelium in asafoetide plant roots base portion tissue
Field planting and fruiting, be of great significance to the child care on the spot and its ecological protection of Pleurotus nebrodensis wild resource.
Research team of the present invention discloses the patent CN106613351A declared, a kind of Pleurotus ferulae on December 26th, 2016
The method of liquid spawn field inoculation asafoetide plant discloses the preparation of asafoetide mushroom liquid strain and Pleurotus ferulae liquid bacteria in the invention
The method of kind field inoculation asafoetide plant, the liquid spawn of this method preparation is in field inoculation asafoetide plant process, due to dagger-axe
Wall beach drought, day temperature is higher, causes partially liq strain to inactivate, influences mycelial field planting success rate and stabilization
Property, therefore this team draws on the experience lesson, with regard to Pleurotus nebrodensis field, child care fruiting technical method is innovated on the spot.
Summary of the invention
For in the prior art only with the preparation of asafoetide mushroom liquid strain and the plant of asafoetide mushroom liquid strain field inoculation asafoetide
The liquid spawn of the method preparation of object is in field inoculation asafoetide plant process, liquid spawn easy in inactivation, and mycelia field planting rate is unstable
Fixed phenomenon has no the state of the art about Pleurotus nebrodensis germ plasm resource field child care fruiting on the spot, the present invention is intended to provide a kind of
The method of Pleurotus nebrodensis field child care fruiting on the spot activates preparation solid spawn by using Pleurotus nebrodensis strain, by Pleurotus nebrodensis solid
Strain field inoculation establishes the technology of Pleurotus nebrodensis solid spawn field inoculation asafoetide plant in asafoetide plant root, and realizes
Field planting and fruiting of the Pleurotus ferulae mycelium in asafoetide plant roots base portion tissue, on Pleurotus nebrodensis germplasm field on the spot child care technology
It makes important progress, provides important technology guidance for the child care on the spot and its ecological protection of Pleurotus nebrodensis germ plasm resource, make Bai Ling
Mushroom germ plasm resource is able to protect and develop, and has wide applicability in terms of the protection of Pleurotus nebrodensis germ plasm resource.
In order to achieve the above object, the present invention is to be achieved through the following technical solutions.
The present invention specifically provides a kind of method of Pleurotus nebrodensis field child care fruiting on the spot, comprising the following steps:
(1) Pleurotus nebrodensis strain activates, and takes out Pleurotus nebrodensis strain from 4 DEG C of safes, and under gnotobasis, picking soybean grain is big
Small pure culture biscuits involvng inoculation is white after being activated in 22 DEG C~26 DEG C incubator 7~10d of dark culture to PDA culture medium plate center
Clever mycelium；
(2) prepared by Pleurotus nebrodensis parent species, the picking soybean grain size from the Pleurotus ferulae mycelium after activation prepared by step (1)
Pure culture biscuits involvng inoculation to PDA slant medium center, in 22 DEG C~26 DEG C biochemical cultivation case dark cultures, cover with inclined-plane to mycelia, obtain
Obtain Pleurotus nebrodensis parent species；
(3) prepared by Pleurotus nebrodensis original seed, the fungus block of 10 pieces of niblet sizes of picking from Pleurotus nebrodensis parent species prepared by step (2)
It is seeded in wheat culture medium, in humidity 50%-65%, the growth cabinet dark culture that 22 DEG C~26 DEG C of temperature is long to mycelia
After full bacterium bag, Pleurotus nebrodensis original seed is obtained；
(4) prepared by Cultivation of Pleurotus nebrodensis kind, with inoculation shovel picking barley inoculum 20- from Pleurotus nebrodensis original seed prepared by step (3)
30 are seeded in Cultivar culture medium, in temperature be 22 DEG C~26 DEG C, humidity be 50%~65% culturing room's dark culture, to
After mycelia covers with bacterium bag, Cultivation of Pleurotus nebrodensis kind is obtained, that is, is used for the solid spawn of field inoculation asafoetide plant；
(5) asafoetide Plant choosing observes asafoetide plant and its rhizome portion, selects non-bolting, 2-3 is raw, and root does not have
The asafoetide plant of Pleurotus nebrodensis mycelia field planting；
(6) asafoetide plant root is handled, and asafoetide foundation portion soil tool is carefully dug and is opened, depth 17-20cm, and radius is
8-12cm exposes asafoetide foundation portion, not destroy the rotten root in asafoetide foundation portion；
(7) Cultivation of Pleurotus nebrodensis kind is inoculated with asafoetide plant, and the Cultivation of Pleurotus nebrodensis kind 80-120g for taking step (4) to prepare is divided into 2-
It 4 pieces, is uniformly put around asafoetide foundation portion, Cultivation of Pleurotus nebrodensis kind is organized against foundation portion, is in close contact strain and foundation portion；
(8) asafoetide plant root post-processes, and will initially dig the soil opened and is all backfilled to asafoetide foundation portion, Pleurotus nebrodensis is planted
It cultivates and is all covered under soil, earthing is gently compacted, then water 80-120ml.
Preferably, Pleurotus nebrodensis strain activates the Pleurotus nebrodensis in 24 DEG C of incubator dark culture 8d, after being activated in the present invention
Mycelium.
Preferably, Pleurotus nebrodensis parent species preparation in the present invention, in 24 DEG C of biochemical cultivation case dark cultures, covers with inclined-plane to mycelia,
Obtain Pleurotus nebrodensis parent species.
Preferably, Pleurotus nebrodensis original seed preparation in the present invention, in humidity 55%-60%, the growth cabinet that 24 DEG C of temperature is dark
Culture obtains Pleurotus nebrodensis original seed after mycelia covers with bacterium bag.
Preferably, in the present invention prepared by Cultivation of Pleurotus nebrodensis kind, and picking barley inoculum 25 are seeded to cultivation from Pleurotus nebrodensis original seed
It cultivates in culture medium, is 24 DEG C in temperature, culturing room's dark culture that humidity is 55%~60% obtains after mycelia covers with bacterium bag
Obtain Cultivation of Pleurotus nebrodensis kind.
Preferably, Cultivation of Pleurotus nebrodensis kind is inoculated with asafoetide plant in the present invention, takes Cultivation of Pleurotus nebrodensis kind 100g, is divided into 3 pieces, encloses
It is uniformly put around asafoetide foundation portion, Cultivation of Pleurotus nebrodensis kind is organized against foundation portion, is in close contact strain and foundation portion.
By using the technical solution of above-mentioned offer, the present invention obtain it is following the utility model has the advantages that
(1) method of a kind of Pleurotus nebrodensis field provided by the invention child care fruiting on the spot, it is living by using Pleurotus nebrodensis strain
Change preparation solid spawn, by Pleurotus nebrodensis solid spawn field inoculation in asafoetide plant root, it is wild to establish Pleurotus nebrodensis solid spawn
The technology of outer inoculation asafoetide plant, and field planting and fruiting of the Pleurotus ferulae mycelium in asafoetide plant roots base portion tissue are realized, it puts down
For field planting rate up to 88.29%, average fructification incidence realizes the field fruiting of Pleurotus nebrodensis up to 28.4%；In Pleurotus nebrodensis germplasm
Child care technically makes important progress on the spot in field, provides weight for the child care on the spot and its ecological protection of Pleurotus nebrodensis germ plasm resource
Technological guidance is wanted, Pleurotus nebrodensis germ plasm resource is enable to protect and develop, is had in terms of the protection of Pleurotus nebrodensis germ plasm resource extensive
Applicability.
(2) method of a kind of Pleurotus nebrodensis field provided by the invention child care fruiting on the spot, by preparing Cultivation of Pleurotus nebrodensis kind,
Pleurotus nebrodensis solid spawn is obtained, the mycelium on strain is able to maintain the vigor of long period, and mycelium is thicker in fungus block, benefit
In the field planting of Pleurotus nebrodensis field and child care fruiting.
(3) method of a kind of Pleurotus nebrodensis field provided by the invention child care fruiting on the spot, in Pleurotus nebrodensis solid spawn field
It is inoculated in asafoetide plant process, asafoetide plant root digs the depth for opening 17-20cm, is easy to the water conservation of Pleurotus nebrodensis solid spawn, makes bacterium
Filament is able to maintain the vigor of long period, is conducive to the field planting of Pleurotus nebrodensis field and child care fruiting.
Detailed description of the invention
Attached drawing 1 show the mycelium figure of Pleurotus nebrodensis activation.
Attached drawing 2 show Pleurotus nebrodensis parent species figure.
Attached drawing 3 show Pleurotus nebrodensis original seed figure.
Attached drawing 4 show Cultivation of Pleurotus nebrodensis kind figure.
Attached drawing 5 show Cultivation of Pleurotus nebrodensis kind inoculation asafoetide plant figure.
Attached drawing 6 show field planting and fruiting figure of the Pleurotus ferulae mycelium in asafoetide plant roots base portion tissue；Wherein, solid line arrow
The white area that head refers to is the Pleurotus ferulae mycelium for being colonized in asafoetide plant roots stem, and the white area that dotted arrow refers to is Bai Ling
Mushroom solid spawn and its mycelium, the white object that hollow arrow refers to are Pleurotus nebrodensis sporophore.
Specific embodiment
In order to which technical problems, technical solutions and advantages to be solved are more clearly understood, tie below
Embodiment is closed, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only used to solve
The present invention is released, is not intended to limit the present invention.Below with reference to the embodiment technical solution that the present invention will be described in detail, but protection scope
It is not limited by this.
Main medium:
PDA culture medium flat panel configurations method: potato 200g is thinly sliced, and is boiled in suitable quantity of water to gently pressing frangible journey
Then glucose 20g, agar powder 20g is added in degree, 2 to 4 layers of filtered through gauze of use, taking juice, then moisturizing is settled to 1000mL, shakes
Packing after even while hot uses culture medium down in culture dish in 115 DEG C of high pressure steam sterilization 30min after culture medium solidification.
Wheat culture medium preparation method: taking the preferable wheat 1kg of integrity degree, removes impurity, is put into basin, is added 2 times
The clear water of wheat volume, impregnate 18 in 25 DEG C~35 DEG C incubators~for 24 hours, wheat is taken out, is drained away the water, packing is to polyethylene
(or polypropylene) bacterium bag, every bag of packing volume is the 1/2 to 2/3 of bacterium bag, lid is covered, then in 121 DEG C of high pressure steam sterilizations
120min is used after culture medium is cool.
Cultivar culture medium preparation method: weighing the dry and fresh cotton seed hulls 82.4kg without mildew, sufficiently prewet, then plus
Enter wheat bran 15kg, white granulated sugar 1kg, land plaster 1kg, calcium superphosphate 0.3kg and urea 0.3kg, stir, by water content
It is adjusted to 60%~65%, compost pH value is adjusted to 8~9 with pulverized limestone, packing is into polypropylene bacterium bag after mixing well, sealing
Afterwards for 24 hours in 100 DEG C of sterilizings, it is used after bacterium bag is cool.
Embodiment one:
(1) Pleurotus nebrodensis strain activates, and takes out Pleurotus nebrodensis strain from 4 DEG C of safes, and under gnotobasis, picking soybean grain is big
Small pure culture biscuits involvng inoculation is white after being activated in 22 DEG C~26 DEG C incubator 7~10d of dark culture to PDA culture medium plate center
Clever mycelium, referring to figure 1；
(2) prepared by Pleurotus nebrodensis parent species, the picking soybean grain size from the Pleurotus ferulae mycelium after activation prepared by step (1)
Pure culture biscuits involvng inoculation to PDA slant medium center, in 22 DEG C~26 DEG C biochemical cultivation case dark cultures, cover with inclined-plane to mycelia, obtain
Pleurotus nebrodensis parent species are obtained, referring to shown in attached drawing 2；
(3) prepared by Pleurotus nebrodensis original seed, the fungus block of 10 pieces of niblet sizes of picking from Pleurotus nebrodensis parent species prepared by step (2)
It is seeded in wheat culture medium, in humidity 50%-65%, the growth cabinet dark culture that 22 DEG C~26 DEG C of temperature is long to mycelia
After full bacterium bag, Pleurotus nebrodensis original seed is obtained, referring to shown in attached drawing 3；
(4) prepared by Cultivation of Pleurotus nebrodensis kind, with inoculation shovel picking barley inoculum 20- from Pleurotus nebrodensis original seed prepared by step (3)
30 are seeded in Cultivar culture medium, in temperature be 22 DEG C~26 DEG C, humidity be 50%~65% culturing room's dark culture, to
After mycelia covers with bacterium bag, Cultivation of Pleurotus nebrodensis kind is obtained, that is, the solid spawn of field inoculation asafoetide plant is used for, referring to 4 institute of attached drawing
Show；
(5) asafoetide Plant choosing observes asafoetide plant and its rhizome portion, selects non-bolting, and 2-3 is raw, and root is not white
The asafoetide plant of clever mushroom silk field planting；
(6) asafoetide plant root is handled, and asafoetide foundation portion soil tool is carefully dug and is opened, depth 17-20cm, radius 8-
12cm exposes asafoetide foundation portion, is careful not to destroy the rotten root in asafoetide foundation portion；
(7) Cultivation of Pleurotus nebrodensis kind is inoculated with asafoetide plant, and the Cultivation of Pleurotus nebrodensis kind 80-120g for taking step (4) to prepare is divided into 2-
It 4 pieces, is uniformly put around asafoetide foundation portion, Cultivation of Pleurotus nebrodensis kind is organized against foundation portion, and it is in close contact strain and foundation portion,
Referring to shown in attached drawing 5；
(8) asafoetide plant root post-processes, and will initially dig the soil opened and is all backfilled to asafoetide foundation portion, Pleurotus nebrodensis is planted
It cultivates and is all covered under soil, earthing is gently compacted, then water 80-120ml.
Embodiment two:
(1) Pleurotus nebrodensis strain activates, and takes out Pleurotus nebrodensis strain from 4 DEG C of safes, and under gnotobasis, picking soybean grain is big
Small pure culture biscuits involvng inoculation is central to PDA culture medium plate, the Pleurotus nebrodensis mycelia in 22 DEG C of incubator dark culture 7d, after being activated
Body；
(2) prepared by Pleurotus nebrodensis parent species, the picking soybean grain size from the Pleurotus ferulae mycelium after activation prepared by step (1)
Pure culture biscuits involvng inoculation to PDA slant medium center, in 22 DEG C of biochemical cultivation case dark cultures, cover with inclined-plane to mycelia, obtain white spirit
Mushroom parent species；
(3) prepared by Pleurotus nebrodensis original seed, the fungus block of 10 pieces of niblet sizes of picking from Pleurotus nebrodensis parent species prepared by step (2)
It is seeded in wheat culture medium, in humidity 50%-55%, 22 DEG C of temperature of growth cabinet dark culture covers with bacterium bag to mycelia
Afterwards, Pleurotus nebrodensis original seed is obtained；
(4) prepared by Cultivation of Pleurotus nebrodensis kind, with inoculation shovel picking barley inoculum 20 from Pleurotus nebrodensis original seed prepared by step (3)
It is seeded in Cultivar culture medium, is 22 DEG C in temperature, culturing room's dark culture that humidity is 50%~55% covers with bacterium to mycelia
After bag, Cultivation of Pleurotus nebrodensis kind is obtained, that is, is used for the solid spawn of field inoculation asafoetide plant；
(5) asafoetide Plant choosing observes asafoetide plant and its rhizome portion, selects non-bolting, and 2-3 is raw, and root is not white
The asafoetide plant of clever mushroom silk field planting；
(6) asafoetide plant root is handled, and asafoetide foundation portion soil tool is carefully dug and is opened, depth 17-20cm, radius 8-
12cm exposes asafoetide foundation portion, is careful not to destroy the rotten root in asafoetide foundation portion；
(7) Cultivation of Pleurotus nebrodensis kind is inoculated with asafoetide plant, and the Cultivation of Pleurotus nebrodensis kind 80g for taking step (4) to prepare is divided into 2 pieces, encloses
It is uniformly put around asafoetide foundation portion, Cultivation of Pleurotus nebrodensis kind is organized against foundation portion, is in close contact strain and foundation portion；
(8) asafoetide plant root post-processes, and will initially dig the soil opened and is all backfilled to asafoetide foundation portion, Pleurotus nebrodensis is planted
It cultivates and is all covered under soil, earthing is gently compacted, then water 80-120ml.
Embodiment three:
(1) Pleurotus nebrodensis strain activates, and takes out Pleurotus nebrodensis strain from 4 DEG C of safes, and under gnotobasis, picking soybean grain is big
Small pure culture biscuits involvng inoculation is central to PDA culture medium plate, the Pleurotus nebrodensis mycelia in 24 DEG C of incubator dark culture 8d, after being activated
Body；
(2) prepared by Pleurotus nebrodensis parent species, the picking soybean grain size from the Pleurotus ferulae mycelium after activation prepared by step (1)
Pure culture biscuits involvng inoculation to PDA slant medium center, in 24 DEG C of biochemical cultivation case dark cultures, cover with inclined-plane to mycelia, obtain white spirit
Mushroom parent species；
(3) prepared by Pleurotus nebrodensis original seed, the fungus block of 10 pieces of niblet sizes of picking from Pleurotus nebrodensis parent species prepared by step (2)
It is seeded in wheat culture medium, in humidity 55%-60%, 24 DEG C of temperature of growth cabinet dark culture covers with bacterium bag to mycelia
Afterwards, Pleurotus nebrodensis original seed is obtained；
(4) prepared by Cultivation of Pleurotus nebrodensis kind, with inoculation shovel picking barley inoculum 25 from Pleurotus nebrodensis original seed prepared by step (3)
It is seeded in Cultivar culture medium, is 24 DEG C in temperature, culturing room's dark culture that humidity is 55%~60% covers with bacterium to mycelia
After bag, Cultivation of Pleurotus nebrodensis kind is obtained, that is, is used for the solid spawn of field inoculation asafoetide plant；
(5) asafoetide Plant choosing observes asafoetide plant and its rhizome portion, selects non-bolting, and 2-3 is raw, and root is not white
The asafoetide plant of clever mushroom silk field planting；
(6) asafoetide plant root is handled, and asafoetide foundation portion soil tool is carefully dug and is opened, depth 17-20cm, radius 8-
12cm exposes asafoetide foundation portion, is careful not to destroy the rotten root in asafoetide foundation portion；
(7) Cultivation of Pleurotus nebrodensis kind is inoculated with asafoetide plant, and the Cultivation of Pleurotus nebrodensis kind 100g for taking step (4) to prepare is divided into 3 pieces,
It is uniformly put around asafoetide foundation portion, Cultivation of Pleurotus nebrodensis kind is organized against foundation portion, is in close contact strain and foundation portion；
(8) asafoetide plant root post-processes, and initially digs the soil opened and is all backfilled to asafoetide foundation portion, and mushroom cultivar is complete
Portion is covered under soil, earthing is gently compacted, then water 80-120ml.
Example IV:
(1) Pleurotus nebrodensis strain activates, and takes out Pleurotus nebrodensis strain from 4 DEG C of safes, and under gnotobasis, picking soybean grain is big
Small pure culture biscuits involvng inoculation is central to PDA culture medium plate, the Pleurotus nebrodensis mycelia in 26 DEG C of incubator dark culture 10d, after being activated
Body；
(2) prepared by Pleurotus nebrodensis parent species, the picking soybean grain size from the Pleurotus ferulae mycelium after activation prepared by step (1)
Pure culture biscuits involvng inoculation to PDA slant medium center, in 26 DEG C of biochemical cultivation case dark cultures, cover with inclined-plane to mycelia, obtain white spirit
Mushroom parent species；
(3) prepared by Pleurotus nebrodensis original seed, the fungus block of 10 pieces of niblet sizes of picking from Pleurotus nebrodensis parent species prepared by step (2)
It is seeded in wheat culture medium, in humidity 60%-65%, 26 DEG C of temperature of growth cabinet dark culture covers with bacterium bag to mycelia
Afterwards, Pleurotus nebrodensis original seed is obtained；
(4) prepared by Cultivation of Pleurotus nebrodensis kind, with inoculation shovel picking barley inoculum 30 from Pleurotus nebrodensis original seed prepared by step (3)
It is seeded in Cultivar culture medium, is 26 DEG C in temperature, culturing room's dark culture that humidity is 60%~65% covers with bacterium to mycelia
After bag, Cultivation of Pleurotus nebrodensis kind is obtained, that is, is used for the solid spawn of field inoculation asafoetide plant；
(5) asafoetide Plant choosing observes asafoetide plant and its rhizome portion, selects non-bolting, and 2-3 is raw, and root is not white
The asafoetide plant of clever mushroom silk field planting；
(6) asafoetide plant root is handled, and asafoetide foundation portion soil tool is carefully dug and is opened, depth 17-20cm, radius 8-
12cm exposes asafoetide foundation portion, is careful not to destroy the rotten root in asafoetide foundation portion；
(7) Cultivation of Pleurotus nebrodensis kind is inoculated with asafoetide plant, and the Cultivation of Pleurotus nebrodensis kind 120g for taking step (4) to prepare is divided into 4 pieces,
It is uniformly put around asafoetide foundation portion, Cultivation of Pleurotus nebrodensis kind is organized against foundation portion, is in close contact strain and foundation portion；
(8) asafoetide plant root post-processes, and will initially dig the soil opened and is all backfilled to asafoetide foundation portion, Pleurotus nebrodensis is planted
It cultivates and is all covered under soil, earthing is gently compacted, then water 80-120ml.
Embodiment five:
Probe into four factors for influencing Pleurotus nebrodensis field planting rate (Y): Pleurotus nebrodensis cultivation temperature (A), Pleurotus nebrodensis culture humidity
(B), the amount (D) of Pleurotus nebrodensis original seed picking wheat number (C) and Cultivation of Pleurotus nebrodensis kind inoculation asafoetide plant, every group of field planting 30 Ah
Wei plant, carries out the experiment of four factors, three horizontal quadrature, and orthogonal test factor and water-glass are shown in Table 1.
Table 1: orthogonal test factor and water-glass
Orthogonal optimization Pleurotus nebrodensis field planting rate test result and analysis are shown in Table 2.
Table 2: orthogonal experiments and analysis
From table 2 it can be seen that Pleurotus nebrodensis original seed picking wheat number is to Pleurotus nebrodensis field planting rate in the range of experimental design
Influence it is maximum.Obtaining optimum optimizing condition in conjunction with Tables 1 and 2 is A2B2C2D2, i.e. 24 DEG C of Pleurotus nebrodensis cultivation temperature, Pleurotus nebrodensis
The amount 100g that humidity 55-60%, Pleurotus nebrodensis original seed picking wheat number 25, Cultivation of Pleurotus nebrodensis kind are inoculated with asafoetide plant is cultivated,
Pleurotus nebrodensis field planting rate is up to 88.2%.
Pass through the method that the above optimum experimental obtains Pleurotus nebrodensis field child care fruiting: taking out Pleurotus nebrodensis from 4 DEG C of safes
Strain, under gnotobasis, the pure culture biscuits involvng inoculation of picking soybean grain size is secretly trained to PDA culture medium plate center in 24 DEG C of incubators
Support 8d, the Pleurotus ferulae mycelium after being activated, the pure culture biscuits involvng inoculation of the Pleurotus nebrodensis soybean grain size after picking to the inclined-plane PDA training
Base center is supported, in 24 DEG C of biochemical cultivation case dark cultures, inclined-plane is covered with to mycelia, Pleurotus nebrodensis parent species is obtained, from Pleurotus nebrodensis parent species
The fungus block of 10 pieces of niblet sizes of picking is seeded in wheat culture medium, in humidity 55-60%, the growth cabinet that 24 DEG C of temperature
Dark culture obtains Pleurotus nebrodensis original seed after mycelia covers with bacterium bag, is connect for picking barley inoculum 25 from Pleurotus nebrodensis original seed with inoculation shovel
Kind is 24 DEG C in temperature into Cultivar culture medium, and humidity is culturing room's dark culture of 55-60%, after mycelia covers with bacterium bag,
Cultivation of Pleurotus nebrodensis kind is obtained, non-bolting is selected, 2-3 is raw, the asafoetide plant that root does not have Pleurotus nebrodensis mycelia to be colonized；By asafoetide root
Base portion soil tool, which is carefully dug, to be opened, depth 17-20cm, radius 8-12cm, exposes asafoetide foundation portion, is careful not to destroy asafoetide
The rotten root in foundation portion；Cultivation of Pleurotus nebrodensis kind 100g is taken, is divided into 3 pieces, is uniformly put around asafoetide foundation portion, Cultivation of Pleurotus nebrodensis kind is tight
It is organized by foundation portion, is in close contact strain and foundation portion, will initially dig the soil opened and be all backfilled to asafoetide foundation portion, by mushroom
Cultivar is all covered under soil, earthing is gently compacted, then water 80-120ml.
Embodiment six:
This programme designs 2 groups of experiments, and A group carries out Pleurotus nebrodensis solid spawn field inoculation asafoetide by the scheme of embodiment one and plants
Object test, B group is by the field inoculation method disclosed on December 26th, 2016 in the patent CN106613351A declared to Pleurotus nebrodensis
Asafoetide vegetal tests are inoculated with, selection is inoculated in April in spring to May and autumn September part to October.
The asafoetide plant that annual spring, autumn carried out Pleurotus nebrodensis solid spawn field inoculation to first 1 year is investigated, main to adjust
The field planting and fruiting situation for looking into Pleurotus ferulae mycelium observe whether test asafoetide plant roots stem has Bai Ling first with ocular estimate
Massee fruiting bodies occur, if not having fructification, then carry out Pleurotus ferulae mycelium and determine in asafoetide plant roots base portion tissue
Plant condition survey, colonisation investigation method are as follows: the soil of asafoetide foundation portion side plane is opened with tool, clean out expose Ah
Wei Gen is careful not to injure asafoetide foundation portion tissue, observation and the field planting of record asafoetide foundation portion Pleurotus nebrodensis mycelia and growth feelings
Condition.
Table 3: Pleurotus nebrodensis field inoculation asafoetide vegetal tests
Ginseng is shown in Table 3, and mycelium colonisation investigation result is shown: A group was successfully determined in Pleurotus ferulae mycelium in 2017
Up to 83.33%, 2018 year, successfully field planting rate was up to 93.25% to plant rate, and averagely field planting rate is up to 88.29%, and a situation arises for fructification
Investigation result is shown: Ah's Pleurotus nebrodensis sporophore occurs 49 within 2017, and incidence was sent out up to 27.3%, 2018 year Pleurotus nebrodensis sporophore
59 raw, incidence is up to 29.5%, and average Pleurotus nebrodensis sporophore incidence is up to 28.4%, and Pleurotus nebrodensis mycelia is colonized and fruiting situation
Referring to shown in attached drawing 6.And successfully up to 54.32%, 2018 year, successfully field planting rate was up to field planting rate B group Pleurotus ferulae mycelium in 2017
60.95%, averagely field planting rate is up to 57.64%.Field planting rate of the scheme provided by the present application in the wild inoculation asafoetide plant of Pleurotus nebrodensis
It is apparently higher than the well-known technique of B group.Test result shows can be with using Pleurotus nebrodensis solid spawn field inoculation asafoetide plant method
The field planting rate of Pleurotus nebrodensis mycelia raw asafoetide plant root out of office is effectively improved, and the generation of Pleurotus nebrodensis sporophore can be effectively improved
Rate realizes the field fruiting of Pleurotus nebrodensis.
As described above, the present invention can be realized preferably, the above embodiments are only to preferred implementation side of the invention
Formula is described, and is not intended to limit the scope of the present invention, and without departing from the spirit of the design of the present invention, this field is general
The various changes and improvement that logical technical staff makes technical solution of the present invention, should all fall into present invention determine that protection scope
It is interior.
Claims (6)
1. a kind of method of Pleurotus nebrodensis field child care fruiting on the spot, which comprises the following steps:
(1) Pleurotus nebrodensis strain activates, and takes out Pleurotus nebrodensis strain from 4 DEG C of safes, under gnotobasis, picking soybean grain size
Pure culture biscuits involvng inoculation is central to PDA culture medium plate, the Pleurotus nebrodensis in 22 DEG C~26 DEG C incubator 7~10d of dark culture, after being activated
Mycelium；
(2) prepared by Pleurotus nebrodensis parent species, the bacterium of picking soybean grain size from the Pleurotus ferulae mycelium after activation prepared by step (1)
Cake is seeded to PDA slant medium center, in 22 DEG C~26 DEG C biochemical cultivation case dark cultures, covers with inclined-plane to mycelia, obtains white
Clever mushroom parent species；
(3) prepared by Pleurotus nebrodensis original seed, the fungus block inoculation of 10 pieces of niblet sizes of picking from Pleurotus nebrodensis parent species prepared by step (2)
Into wheat culture medium, in humidity 50%-65%, the growth cabinet dark culture that 22 DEG C~26 DEG C of temperature covers with bacterium bag to mycelia
Afterwards, Pleurotus nebrodensis original seed is obtained；
(4) prepared by Cultivation of Pleurotus nebrodensis kind, with inoculation shovel picking barley inoculum 20-30 from Pleurotus nebrodensis original seed prepared by step (3)
It is seeded in Cultivar culture medium, is 22 DEG C~26 DEG C in temperature, culturing room's dark culture that humidity is 50%~65% is long to mycelia
After full bacterium bag, Cultivation of Pleurotus nebrodensis kind is obtained, that is, is used for the solid spawn of field inoculation asafoetide plant；
(5) asafoetide Plant choosing observes asafoetide plant and its rhizome portion, selects non-bolting, 2-3 is raw, and root does not have white spirit
The asafoetide plant of mushroom silk field planting；
(6) asafoetide plant root is handled, and asafoetide foundation portion soil tool is carefully dug and is opened, depth 17-20cm, radius 8-
12cm exposes asafoetide foundation portion, not destroy the rotten root in asafoetide foundation portion；
(7) Cultivation of Pleurotus nebrodensis kind is inoculated with asafoetide plant, and the Cultivation of Pleurotus nebrodensis kind 80-120g for taking step (4) to prepare is divided into 2-4 block,
It is uniformly put around asafoetide foundation portion, Cultivation of Pleurotus nebrodensis kind is organized against foundation portion, is in close contact strain and foundation portion；
(8) asafoetide plant root post-processes, and will initially dig the soil opened and is all backfilled to asafoetide foundation portion, by Cultivation of Pleurotus nebrodensis kind
It is all covered under soil, earthing is gently compacted, then water 80-120ml.
2. a kind of method of Pleurotus nebrodensis field according to claim 1 child care fruiting on the spot, which is characterized in that Pleurotus nebrodensis
Pleurotus ferulae mycelium of the kind activation in 24 DEG C of incubator dark culture 8d, after being activated.
3. a kind of method of Pleurotus nebrodensis field according to claim 1 child care fruiting on the spot, which is characterized in that Pleurotus nebrodensis is female
Prepared by kind, in 24 DEG C of biochemical cultivation case dark cultures, cover with inclined-plane to mycelia, obtain Pleurotus nebrodensis parent species.
4. a kind of method of Pleurotus nebrodensis field according to claim 1 child care fruiting on the spot, which is characterized in that Pleurotus nebrodensis is former
Prepared by kind, in humidity 55%-60%, it is former to obtain Pleurotus nebrodensis after mycelia covers with bacterium bag for 24 DEG C of temperature of growth cabinet dark culture
Kind.
5. a kind of method of Pleurotus nebrodensis field according to claim 1 child care fruiting on the spot, which is characterized in that Pleurotus nebrodensis is planted
Preparation is cultivated, picking barley inoculum 25 are seeded in Cultivar culture medium from Pleurotus nebrodensis original seed, are 24 DEG C in temperature, humidity is
55%~60% culturing room's dark culture obtains Cultivation of Pleurotus nebrodensis kind after mycelia covers with bacterium bag.
6. a kind of method of Pleurotus nebrodensis field according to claim 1 child care fruiting on the spot, which is characterized in that Pleurotus nebrodensis is planted
Inoculation asafoetide plant is cultivated, Cultivation of Pleurotus nebrodensis kind 100g is taken, is divided into 3 pieces, uniformly put around asafoetide foundation portion, Cultivation of Pleurotus nebrodensis
Kind is organized against foundation portion, is in close contact strain and foundation portion.
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