CN116814496A - Bacteria capable of dissolving phosphorus efficiently and promoting plant growth and application thereof - Google Patents
Bacteria capable of dissolving phosphorus efficiently and promoting plant growth and application thereof Download PDFInfo
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- 241000894006 Bacteria Species 0.000 title claims abstract description 34
- 230000001737 promoting effect Effects 0.000 title claims abstract description 14
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 title claims abstract description 10
- 239000011574 phosphorus Substances 0.000 title claims abstract description 10
- 229910052698 phosphorus Inorganic materials 0.000 title claims abstract description 10
- 230000008635 plant growth Effects 0.000 title claims abstract description 10
- 230000001580 bacterial effect Effects 0.000 claims abstract description 40
- 239000007788 liquid Substances 0.000 claims abstract description 34
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims abstract description 26
- 241000208125 Nicotiana Species 0.000 claims abstract description 25
- 238000000855 fermentation Methods 0.000 claims abstract description 22
- 230000004151 fermentation Effects 0.000 claims abstract description 22
- 241000209082 Lolium Species 0.000 claims abstract description 21
- 241000588626 Acinetobacter baumannii Species 0.000 claims abstract description 18
- 238000004321 preservation Methods 0.000 claims abstract description 6
- 244000005700 microbiome Species 0.000 claims abstract description 4
- 239000000843 powder Substances 0.000 claims description 30
- 239000001963 growth medium Substances 0.000 claims description 20
- 241000196324 Embryophyta Species 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 12
- 238000009630 liquid culture Methods 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 239000002609 medium Substances 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 239000012137 tryptone Substances 0.000 claims description 3
- 230000012010 growth Effects 0.000 abstract description 13
- 238000004519 manufacturing process Methods 0.000 abstract description 11
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000008901 benefit Effects 0.000 abstract description 2
- 238000009335 monocropping Methods 0.000 abstract description 2
- 239000003337 fertilizer Substances 0.000 description 10
- 238000001035 drying Methods 0.000 description 8
- 239000002689 soil Substances 0.000 description 8
- 230000000694 effects Effects 0.000 description 6
- 235000015099 wheat brans Nutrition 0.000 description 6
- 239000000203 mixture Substances 0.000 description 5
- 238000011161 development Methods 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 230000003213 activating effect Effects 0.000 description 3
- 238000012271 agricultural production Methods 0.000 description 3
- 230000004720 fertilization Effects 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000007664 blowing Methods 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 238000003973 irrigation Methods 0.000 description 2
- 230000002262 irrigation Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 240000008027 Akebia quinata Species 0.000 description 1
- 235000007756 Akebia quinata Nutrition 0.000 description 1
- 244000020518 Carthamus tinctorius Species 0.000 description 1
- 235000003255 Carthamus tinctorius Nutrition 0.000 description 1
- 240000004296 Lolium perenne Species 0.000 description 1
- 241000219745 Lupinus Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000013585 weight reducing agent Substances 0.000 description 1
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G20/00—Cultivation of turf, lawn or the like; Apparatus or methods therefor
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/45—Tobacco
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
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- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The application relates to a bacterium which is capable of dissolving phosphorus efficiently and promoting plant growth, wherein the bacterium is Acinetobacter baumannii Acinetobacter baumannii BJ-2-17; the preservation place is the Guangdong province microorganism strain preservation center; the preservation time is 2023, 6 and 1; the deposit number is GDMCC No.63519. The application has the beneficial effects that (1) compared with other modes for eliminating continuous cropping obstacle, the fermentation broth prepared by the application has the characteristics of green, safety and environmental protection. (2) The bacterial liquid prepared by the application has good growth promoting effect on ryegrass and tobacco, can be applied to the production of pasture, increases the yield and improves the economic benefit.
Description
Technical Field
The application belongs to the technical field of bacterial application research, and particularly relates to a bacterium capable of efficiently dissolving phosphorus and promoting plant growth and the technical field of application of the bacterium.
Background
In agricultural production, chemical fertilizers are one of important production data and are important for crop growth and agricultural development. Since the fifth sixty of the last century, china begins to apply fertilizers in large quantities to meet the demands of crops on nutrients, the dosage of the fertilizers is obviously increased, the problems of unreasonable fertilization and excessive fertilization are increasingly prominent, and fertilizer waste, utilization rate reduction, environmental pollution and the like are caused.
With the development of modern agriculture, the method is green and environment-friendly, and sustainable development becomes a main melody of the development of the agriculture. In the agricultural production process, a series of soil, production and environmental problems such as soil hardening, fertilizer waste, fertilizer efficiency reduction, environmental pollution and the like are caused by long-term massive fertilization, and weight reduction and synergy are needed. Microbial fertilizer is a product which causes crops to obtain a specific fertilizer effect by the vital activity of microorganisms, and is one of fertilizers used in agricultural production. It has been in China for about 50 years, and can improve crop nutrition condition, create good soil micro-ecological environment, increase quick-acting phosphorus and potassium content in soil and promote crop growth.
The most important link in the production of microbial fertilizers is to screen strains with the effect of promoting plant growth.
Disclosure of Invention
The application aims to solve the defects, and provides a bacterium for promoting plant growth and application thereof. According to the application, a strain of Acinetobacter baumannii (Acinetobacter baumannii) which is efficient in phosphorus dissolution and has a growth promoting effect on crops is screened out, and the strain is named as BJ-2-17.
The application is realized by adopting the following technical scheme.
The application relates to a bacterium which is capable of dissolving phosphorus efficiently and promoting plant growth, wherein the bacterium is Acinetobacter baumannii Acinetobacter baumannii BJ-2-17; the preservation place is the Guangdong province microorganism strain preservation center; the preservation time is 2023, 6 and 1; the deposit number is GDMCC No.63519.
The application of the bacteria promotes the increase of the plant height of ryegrass.
The use of the bacteria of the present application promotes an increase in the dry weight of ryegrass.
The application of the bacteria promotes the increase of the stem circumference of tobacco.
The application of the bacteria promotes the increase of the plant height of tobacco.
The application of the bacteria promotes the increase of the maximum waist leaf length of tobacco.
The application of the bacteria promotes the increase of the maximum waist leaf width of tobacco.
The application of the bacteria promotes the increase of the effective leaf number of tobacco.
A method of preparing a bacterial fermentation broth using the above, the method comprising the steps of:
step 1) preparing a culture medium: LB liquid medium: 10g of tryptone, 5g of yeast powder, 10g of NaCl and 1000ml of deionized water.
Step 2), placing the culture medium in the step 1 into an autoclave for sterilization at 121 ℃ for 20min;
step 3) after the culture medium in the step 2 is cooled, inoculating the activated Acinetobacter baumannii Acinetobacter baumannii BJ-2-17 into a liquid culture medium, and placing the liquid culture medium into a shaking table with the speed of 120r/min, wherein the culture temperature is 28 ℃;
step 4) culturing for 2-4d to finish the preparation of the fermentation liquor.
The application has the beneficial effects that (1) compared with other modes for eliminating continuous cropping obstacle, the fermentation broth prepared by the application has the characteristics of green, safety and environmental protection. (2) The bacterial liquid prepared by the application has good growth promoting effect on ryegrass, can be applied to pasture production in future, increases the yield and improves the economic benefit.
The application is further explained below with reference to the drawings and the detailed description.
Drawings
FIG. 1 is a front and back side view of the strain of the present application;
FIG. 2 is a phylogenetic tree of the strain of the present application;
FIG. 3 is a graph showing the effect of the strain in promoting ryegrass growth;
FIG. 4 is a graph showing the effect of the strain of the present application on promoting tobacco growth;
FIG. 5 is a graph showing the effect of phosphorus dissolution of the strain of the present application.
Detailed Description
See fig. 1, 2, 3, 4 and 5.
A fermentation method for promoting plant growth bacteria and its application are disclosed, wherein the fermented bacteria liquid is used for treating ryegrass seedling.
Further, the bacterial strain is Acinetobacter baumannii, and is obtained by screening from the rhizosphere soil of the Aotope pastoris. The collection number of BJ-2-17 (Acinetobacter baumannii) was 62684.
Further, the preparation method of the Acinetobacter baumannii fermentation broth comprises the following steps:
(1) Preparing a culture medium: LB liquid medium: 10g of tryptone, 5g of yeast powder, 10g of NaCl and 1000ml of deionized water. Sterilizing in autoclave at 121deg.C for 20min;
(2) After the medium cooled, the activated cells were inoculated into a liquid medium and placed in a shaking table at 120r/min at a culture temperature of 28 ℃. Culturing for 2-4d;
further, the application mode of the strain is as follows:
(1) During crop cultivation, a cultivation hole corresponding to the seedling root size is dug in soil, a small seedling is placed in the cultivation hole, fermented bacterial liquid is poured on the root of a plant, and the bacterial liquid consumption is 10ml-100 ml/plant according to the root size.
(2) Mixing the fermented bacterial liquid with water for root irrigation in any proportion, and irrigating into cultivation holes or irrigating after earthing during crop cultivation.
(3) And adding the fermented bacterial liquid into the sterilized wheat bran, drying at low temperature to prepare bacterial powder, and applying the dried bacterial powder to the field when cultivating plants.
(4) And adding the fermented bacterial liquid into sterilized wheat bran, drying at low temperature to prepare bacterial powder, activating the bacterial powder in the nutrient solution, and pouring the bacterial powder into the roots of plants during cultivation.
(5) And freeze-drying the fermented bacterial liquid to prepare pure bacterial powder, and directly applying the dried pure bacterial powder when cultivating plants.
(6) And freeze-drying the fermented bacterial liquid to prepare pure bacterial powder, and mixing the pure bacterial powder with other substances for application.
Freeze-drying the fermented bacterial liquid to prepare pure bacterial powder, activating the bacterial powder, and pouring the bacterial liquid containing activated bacteria on the root of the plant.
Example 1: the application of Acinetobacter baumannii BJ-2-17 fermentation liquor in ryegrass production.
Fermenting the strain according to the fermentation condition until the culture medium is obviously turbid, adding the strain into a conventional nutrient soil matrix according to the dosage of 15ml per basin to serve as a treatment group, and simultaneously taking a group without adding bacterial liquid as a control group; firstly, raising seedlings of ryegrass, and using perennial ryegrass lupine as a test material. Firstly, uniformly broadcasting ryegrass seeds in a seedling raising tray, putting the seedling raising tray in a greenhouse for raising seedlings, and transplanting the seedlings of ryegrass with consistent growth vigor, no diseases and no damage into a 13X 12cm nutrition bag when pasture grows to two leaves and is about 10 cm. And 7 seedlings are treated in each treatment, three groups are repeated, the seedlings grow for about one month, various relevant indexes are measured, and the influence of bacterial liquid treatment on the growth of ryegrass is detected.
Example 2: application of Acinetobacter baumannii BJ-2-17 fermentation liquor in ryegrass production after dilution: fermenting the strain according to the fermentation condition until the strain liquid with obvious turbidity appears, respectively diluting the strain liquid by 10 times, 20 times, 30 times, 40 times and 50 times, then irrigating the ryegrass, and applying the rest treatment methods with the fermentation liquid.
Example 3: application of acinetobacter baumannii BJ-2-17 prepared into bacterial powder in ryegrass production: fermenting the strain according to the fermentation condition until obvious turbid bacteria liquid appears, adding the fermented bacteria liquid into wheat bran subjected to high-temperature high-pressure sterilization, adding 1L of strain fermentation liquid into 1kg of wheat bran, fully and uniformly mixing the materials, putting the mixture into an electrothermal blowing drying oven under the condition of 55 ℃, drying the mixture for 3 hours, storing the treated bacteria powder at a normal-temperature drying place, applying the dried bacteria powder to a field when crops are cultivated, wherein the application amount is 50kg per mu of land, and applying the dried bacteria powder in furrows or directly scattering the dried bacteria powder to the field.
Example 4: application of Acinetobacter baumannii BJ-2-17 prepared into bacterial powder in ryegrass production after activation: the method for preparing the bacterial powder is the same as that of example 3, the strain is activated before use, firstly, an LB culture medium is prepared, 5g of bacterial powder is added into each 1L of liquid culture medium, the liquid culture medium is placed into a shaking table, the rotation speed is set to be 120r/min, the culture temperature is 28 ℃, and the liquid culture medium can be used after obvious turbidity appears in 2-4 days of culture. The activated bacterial liquid is diluted by 10 times, 20 times, 30 times, 40 times and 50 times respectively for treating ryegrass, and the ryegrass can be directly root-irrigated according to the dosage of 500ml fermentation liquor per square meter.
The specific measurement indexes and the method are as follows:
plant height: the length from the stem base to the stem tip of the plant was measured with a ruler.
Tillering number: the tillering number was determined by counting.
Fresh weight of aerial parts: fresh weight of the aerial parts of the plants was weighed with a balance.
Fresh weight of underground part: the fresh weight of the underground part of the plant was weighed with a balance.
Example 5: use of Acinetobacter baumannii BJ-2-17 in tobacco production is provided.
Fermenting the strain according to the fermentation condition until the strain liquid with obvious turbidity appears, adding the strain liquid into a conventional nutrient soil matrix according to the dosage of 50ml per basin to serve as a treatment group, and simultaneously taking a group without adding the strain liquid as a control group; firstly, raising seedlings of tobacco, and using a tobacco variety of safflower Dajinyuan as a test material. And transplanting the tobacco seedlings into plastic pot after the tobacco seedlings grow to the five-leaf period, placing one seedling per pot into a greenhouse for cultivation, and treating 7 pots per pot. And sampling tobacco in a vigorous and long-term manner, measuring various related indexes, and detecting the influence of bacterial liquid treatment on tobacco growth.
Example 6: application of Acinetobacter baumannii BJ-2-17 fermentation broth after dilution in tobacco production: fermenting the strain according to the fermentation condition until the strain liquid is obviously turbid, respectively diluting the strain liquid by 10-50 times, and then treating the tobacco, wherein the rest treatment methods are applied with the fermentation liquid.
Example 7: acinetobacter baumannii BJ-2-17 is prepared into bacterial powder for application: fermenting the strain according to the fermentation condition until the strain is obviously turbid, adding the fermented strain into wheat bran subjected to high-temperature and high-pressure sterilization, adding 1L of strain fermentation liquor into one kilogram of wheat bran, fully and uniformly mixing the mixture, putting the mixture into an electrothermal blowing drying oven under the condition of 55 ℃, drying the mixture for 3 hours, storing the treated strain powder at a normal-temperature drying place, applying the dried strain powder to a field when cultivating plants, wherein the application amount is 50 kilograms per mu of land, and applying the strain powder in furrows or directly scattering the strain powder in the field.
Example 8: acinetobacter baumannii BJ-2-17 is prepared into bacterial powder for activating and then is applied: the method for preparing the bacterial powder is the same as that of example 3, the strain is activated before use, firstly, an LB culture medium is prepared, 5g of bacterial powder is added into each 1L of liquid culture medium, the liquid culture medium is placed into a shaking table, the rotation speed is set to be 120r/min, the culture temperature is 28 ℃, and the liquid culture medium can be used after obvious turbidity appears in 2-4 days of culture. The activated bacterial liquid is diluted by 10-50 times respectively, and then the tobacco is treated, and the tobacco can be directly root-irrigated according to the dosage of 10-100ml bacterial liquid per plant.
The data acquisition is carried out in a vigorous period of tobacco plant growth, and specific measurement indexes and methods are as follows:
plant height: the height from the basal part of the plant ground stem to the growing point is measured by a flexible ruler.
The stem circumference: the circumference of the stem from the 5 th leaf position to the 6 th leaf position from the ground.
Maximum leaf length: and measuring the blade base to the blade tip by using a flexible rule to obtain the maximum blade length.
Maximum leaf width: and measuring the widest part of the blade by using a flexible rule to obtain the maximum blade width.
Table 1 various indexes of potted ryegrass treated by root irrigation of strain BJ-2-17
Note that: in the same column of the two-way valve, * indicating significant differences at P < 0.05 levels
TABLE 2 influence of BJ-2-17 Strain on various indices of ryegrass growth in field trials
Note that: in the same column of the two-way valve, * indicating significant differences at P < 0.05 levels
TABLE 3 influence of BJ-2-17 Strain on various indices of tobacco growth in potting experiments
Note that: in the same column of the two-way valve, * indicating significant differences at P < 0.05 levels
TABLE 4 influence of the BJ-2-17 Strain on various indices of tobacco growth in field trials
Note that: in the same column of the two-way valve, ** representing a very significant difference at P < 0.05 level
Table 5 qualitative and quantitative determination of phosphorus dissolving Capacity of Strain BJ-2-17
The foregoing description is only a few specific embodiments of the present application (the embodiments are not intended to be exhaustive, and the scope of the application includes the scope of the application and other technical points), and the details or common sense of the present application are not described in any more detail herein (including but not limited to the shorthand, abbreviations, units commonly used in the art). It should be noted that the above embodiments do not limit the present application in any way, and it is within the scope of the present application for those skilled in the art to obtain the technical solution by equivalent substitution or equivalent transformation. The protection scope of the present application is subject to the content of the claims, and the description of the specific embodiments and the like in the specification can be used for explaining the content of the claims.
Claims (9)
1. A bacterium which is capable of dissolving phosphorus efficiently and promoting plant growth, which is characterized in that the bacterium is Acinetobacter baumannii Acinetobacter baumannii BJ-2-17; the preservation place is the Guangdong province microorganism strain preservation center; the preservation time is 2023, 6 and 1; the deposit number is GDMCC No.63519.
2. The use of the bacterium of claim 1, to promote increased plant height of ryegrass.
3. The use of the bacterium of claim 1 to promote an increase in dry weight of ryegrass.
4. The use of the bacterium of claim 1 to promote increased stem circumference in tobacco.
5. The use of the bacterium of claim 1, to promote an increase in plant height of tobacco.
6. The use of the bacterium of claim 1, to promote an increase in the maximum waist leaf length of tobacco.
7. The use of the bacterium of claim 1, to promote an increase in maximum waist leaflet width of tobacco.
8. The use of the bacterium of claim 1, to promote an increase in the effective leaf number of tobacco.
9. A method of preparing a bacterial fermentation broth according to claim 1, comprising the steps of:
step 1) preparing a culture medium: LB liquid medium: 10g of tryptone, 5g of yeast powder, 10g of NaCl and 1000ml of deionized water.
Step 2), placing the culture medium in the step 1 into an autoclave for sterilization at 121 ℃ for 20min;
step 3) after the culture medium in the step 2 is cooled, inoculating the activated Acinetobacter baumannii Acinetobacter baumannii BJ-2-17 into a liquid culture medium, and placing the liquid culture medium into a shaking table with the speed of 120r/min, wherein the culture temperature is 28 ℃;
step 4) culturing for 2-4d to finish the preparation of the fermentation liquor.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310812536.2A CN116814496A (en) | 2023-07-04 | 2023-07-04 | Bacteria capable of dissolving phosphorus efficiently and promoting plant growth and application thereof |
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|---|---|---|---|
| CN202310812536.2A CN116814496A (en) | 2023-07-04 | 2023-07-04 | Bacteria capable of dissolving phosphorus efficiently and promoting plant growth and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115960765A (en) * | 2022-11-02 | 2023-04-14 | 云南大学 | Flavobacterium bacteria and application thereof in low-temperature composting |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115960765A (en) * | 2022-11-02 | 2023-04-14 | 云南大学 | Flavobacterium bacteria and application thereof in low-temperature composting |
| CN115960765B (en) * | 2022-11-02 | 2024-04-26 | 云南大学 | Flavobacterium strain and application thereof in low-temperature composting |
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