CN107536034A - A kind of multienzyme kudzu root oral liquid and preparation method thereof - Google Patents
A kind of multienzyme kudzu root oral liquid and preparation method thereof Download PDFInfo
- Publication number
- CN107536034A CN107536034A CN201710685282.7A CN201710685282A CN107536034A CN 107536034 A CN107536034 A CN 107536034A CN 201710685282 A CN201710685282 A CN 201710685282A CN 107536034 A CN107536034 A CN 107536034A
- Authority
- CN
- China
- Prior art keywords
- kudzu
- root
- multienzyme
- vine
- oral liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The present invention relates to a kind of multienzyme kudzu root oral liquid and preparation method thereof.The preparation of the multienzyme kudzu root oral liquid includes：1) kudzu-vine root powder is mixed with water, prepares mixed liquor A；2) amylase and activator are added into mixed liquor A, 20min~30min is digested at 60~85 DEG C, obtains mixed liquid B；3) pH value for adjusting mixed liquid B is 8~12, and alkali cellulose enzyme and protease are added into mixed liquid B, and 4~5h is digested at 40~50 DEG C, obtains mixed liquor C；4) beer complex enzyme and distiller's yeast are added into mixed liquor C, anaerobic fermentation is carried out at 35~40 DEG C, obtains zymotic fluid；Pectase is added into zymotic fluid, stirring reaction, filters and produces.The preparation of the multienzyme kudzu root oral liquid while being extracted to medicinal ingredients such as Puerarins, remains the enzymatic activity of a variety of enzymes in the root of kudzu vine, improves the utilization rate of the root of kudzu vine without thermophilic digestion or autoclaving process.
Description
Technical field
The invention belongs to health care oral liquid or OTC field of medicaments, and in particular to a kind of multienzyme kudzu root oral liquid and its system
Preparation Method.
Background technology
The root of kudzu vine is the root of the perennial liana elegant jessamine of pulse family, cool in nature, sweet, pungent, is the medicine-food two-purpose that the Ministry of Public Health is assert
Resource.The root of kudzu vine has rich in materials such as starch, Puerarin, Puerarin -7- xylosides, daizeol, daidzin, arachidic acids
The good reputation of " asia ginseng ".Modern medicine study finds that Puerarin has lowering blood pressure and blood fat, hypoglycemic, expansion blood vessel, increased
Add brain and coronary blood flow, have significant adjuvant treatment effect to hypertension, angina pectoris and coronary heart disease.At present, based on Pueraria lobota
The product of root processing has that a lot, to give full play to the characteristic of the root of kudzu vine be one of mostly important striving direction of R＆D personnel.
Authorization Notice No. be CN102125241B patent disclose a kind of natural enzymolysis Pueraria Mirifica oral liquid and its
Preparation method, it is after Pueraria Mirifica powder is steamed into 20~30min, jar fermenter to be added, with fresh fructus hordei germinatus, water in 60 DEG C of enzymes
Solve, clear liquid is filtrated to get after enzymolysis, after clear liquid is heated into boiling, oral liquid finished product is obtained through cooling, filling, capping.
Existing kudzu root oral liquid focuses mostly on more in the functional enzyme in the extraction and application of the medicinal ingredients such as Puerarin, the root of kudzu vine
Inactivated in boiling or high-temperature sterilization process, the utilization rate and overall efficacy for causing the root of kudzu vine substantially reduce.
The content of the invention
It is an object of the invention to provide a kind of multienzyme kudzu root oral liquid, so as to solve utilization rate of the existing product to the root of kudzu vine
Low, the problem of function is low.
Second object of the present invention is the preparation method for providing above-mentioned multienzyme kudzu root oral liquid.
To achieve the above object, the technical scheme of kudzu root oral liquid of the invention is：
A kind of multienzyme kudzu root oral liquid, is prepared by the method comprised the following steps：
1) kudzu-vine root powder is mixed with water, prepares mixed liquor A；
2) amylase and activator are added into mixed liquor A, 20min~30min is digested at 60~85 DEG C, is mixed
Liquid B；
3) pH value for adjusting mixed liquid B is 8~12, and alkali cellulose enzyme and protease are added into mixed liquid B, 40~
4~5h is digested at 50 DEG C, obtains mixed liquor C；
4) beer complex enzyme and distiller's yeast are added into mixed liquor C, anaerobic fermentation is carried out at 35~40 DEG C, is fermented
Liquid；Pectase is added into zymotic fluid, stirring reaction, filters and produces.
Multienzyme kudzu root oral liquid provided by the invention, it is net that physics is carried out to kudzu-vine root powder using enzymolysis and microorganism fermentation process
Change is handled, and without thermophilic digestion or autoclaving process, while effectively being extracted to medicinal ingredients such as Puerarins, is retained
Superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), glutathione peroxidase in the root of kudzu vine
The enzymatic activity of a variety of enzymes such as enzyme (GSH-PX), these enzymes not only can effectively remove internal unnecessary free radical, can also improve human body
Microenvironment, the functional components such as Puerarin are made to be easier to be absorbed by the body.The kudzu root oral liquid improves the utilization rate of the root of kudzu vine, makes production
The overall efficacy of the root of kudzu vine more highlights in product.
Preferably, the kudzu-vine root powder is formed by the multienzyme root of kudzu vine through crushing, and the multienzyme root of kudzu vine is cultivated to obtain by following methods,
This method includes：After root of kudzu vine final singling, amino acid fertilizer is sprayed to root of kudzu vine root soil, root, stem, leaf site.The amino acid fertilizer
One kind or combination in the amino-acid compound fertilizer that Freamine Ⅲ, the CN103626545B recorded for CN1299688C is recorded.
Preferably, once above-mentioned amino acid fertilizer is sprayed to root of kudzu vine root soil, root, stem, leaf site within every 25~35 days.It is described
Amino acid fertilizer meets GB/T 17419-1998《Containing aminoacid foliar fertilizer》Index request
In addition to aforesaid operations, the cultural method of the multienzyme root of kudzu vine is carried out according to the daily management of the conventional root of kudzu vine.It can such as combine and survey
Soil fertilization supplementary fertilizer technology, biology cure the disease worm technology, artificial weeding means realize fertilising, deinsectization, weeding.
The content of the multienzyme root of kudzu vine for cultivating to obtain using the above method, the not only functional enzyme such as SOD, GSH-PX, POD, CAT
Greatly increase, also rich in multiple elements such as zinc, selenium, strontium, manganese, molybdenum, copper, iron, zinc, boron, magnesium, silicon, potassium, further increase the root of kudzu vine
The effect of and value.
In step 1), the cereal crops rich in SOD are added during mixing, the mass ratio of the cereal crops and kudzu-vine root powder is 1:
1.Grain effect rich in SOD is that following methods cultivate to obtain, and this method includes：In sowing time, using amino acid fertilizer to seed
Soaked seed or dressed seed；In tri-leaf period, jointing stage, boot stage, pustulation period, sprayed to the root soil, stem, leaf site of cereal crops
Spill amino acid fertilizer.The cereal crops can be wheat flour, pearling cone meal, corn flour, Rice Powder or other cereal crops.
The amino acid fertilizer is that the amino acid that Freamine Ⅲ, the CN103626545B that CN1299688C is recorded are recorded is compound
Any one in fertilizer or combination.The above method cultivates obtained crop acceptor, rich in SOD, GSH-PX, CAT and POD, also contains
There are 10 kinds of trace elements such as Zn, Mg, Fe, Se, Sr, Mn, Mo, B, K, Cu；Using above-mentioned cereal crops as raw material, oral liquid can be made
Functional component species is more abundant, nutrient balance, health properties are more preferable.
In step 1), the mass ratio that the kudzu-vine root powder mixes with water is (20~40)：100.
In step 2), the amylase is mesophilicα-diastase, and the activator is calcium chloride；In corresponding per kg kudzu-vine root powders
The addition of warm alpha-amylase is 0.25~0.6ml, and the addition of corresponding calcium chloride is 0.01~0.03g.
In step 2), the addition that protease is corresponded to per kg kudzu-vine root powders is 0.1~0.3kg, corresponding alkali cellulose enzyme
Addition is 0.2~0.5kg；The protease is preferably alkali protease.
In step 2), the addition that beer complex enzyme is corresponded to per kg kudzu-vine root powders is 0.4~1ml, the addition of corresponding distiller's yeast
For 0.8~1.4g；The beer complex enzyme contains the enzyme of 1,4 beta-glucanase, zytase and protease, wherein 1,4 beta-glucanase
Vigor >=8300u/g, enzyme activity >=2800u/g of zytase, enzyme activity >=30000u/g of protease.
The time of the anaerobic fermentation is 6~8 days.Preferably, the 1st~3 day of anaerobic fermentation, led to per 10h~14h
Gas agitating, the 4th day to fermentation ends, air agitation is carried out per 24h.
In step 2), the addition that pectase is corresponded to per kg zymotic fluids is 1~5ml, and the enzyme activity of the pectase is 30,000
u/ml。
The time of the stirring reaction is 90~200min.
Multienzyme kudzu root oral liquid provided by the invention, using multienzyme root of kudzu vine complete stool or root of kudzu vine block as raw material, through enzyme digestion reaction and
After microorganism fermentation process processing, the content of the functional enzyme such as SOD, GSH-PX, POD, CAT greatly increases, and nutritive value enters one
Step improves, and the value of the root of kudzu vine and effect more highlight in product.
The preparation method of the multienzyme kudzu root oral liquid of the present invention, comprises the following steps：
1) kudzu-vine root powder is mixed with water, prepares mixed liquor A；
2) amylase and activator are added into mixed liquor A, 20min~30min is digested at 60~85 DEG C, is mixed
Liquid B；
3) pH value for adjusting mixed liquid B is 8~12, and alkali cellulose enzyme and protease are added into mixed liquid B, 40~
4~5h is digested at 50 DEG C, obtains mixed liquor C；
4) beer complex enzyme and distiller's yeast are added into mixed liquor C, anaerobic fermentation is carried out at 35~40 DEG C, is fermented
Liquid；Pectase is added into zymotic fluid, stirring reaction, filters and produces.
The optimal technical scheme of each step is identical with the technical scheme of above-mentioned multienzyme kudzu root oral liquid, will not be described in detail herein.
The preparation method of the multienzyme kudzu root oral liquid of the present invention, technique is simple, and auxiliary material wide material sources, cost is low, gained production
In product SOD enzymes content >=30u/ml；Preparation process does not need special equipment, and technique is simple, easy to operate, is easy to automate
Control, it is adapted to large-scale industrial production.
Embodiment
Embodiments of the present invention are described further with reference to specific embodiment.In following examples, by with
Lower cultural method plants the multienzyme root of kudzu vine, selects soil depth to reach more than 80cm soil, after excavating kind of planting ditch, uses sufficient base manure, adopts
With kudzu bud section cottage method nurturing staff, 3 year planting season after root of kudzu vine final singling, every 30 days with amino acid foliage fertilizer
800 times of dilutions carry out even spraying to its root soil, stem, leaf site, the daily management that remaining operation is cultivated according to the root of kudzu vine
Carry out, amino acid foliage fertilizer used is the Freamine Ⅲ that CN1299688C is recorded.
The multienzyme root of kudzu vine for cultivating to obtain through the above method, rich in enzymes such as GSH-PX, SOD, POD, CAT, also containing selenium, strontium,
The multiple elements such as manganese, molybdenum, iron, copper, zinc, potassium, silicon, boron, magnesium, improve the medical value of the root of kudzu vine.Above-mentioned cultural method is also suitable
In other medicinal materials, such as pseudo-ginseng, Radix Angelicae Sinensis, cassia seed, mulberry leaf, the Radix Astragali, black nightshade, rhodiola root, matrimony vine, chrysanthemum.
By taking wheat as an example, the cultivation of the cereal crops rich in SOD multienzyme is carried out, the amino acid fertilizer used is CN1299688C
The Freamine Ⅲ of record, in sowing time, dressed seed using 400~800 times of dilutions of Freamine Ⅲ, tri-leaf period,
Jointing stage, boot stage, pustulation period, using 800 times of dilutions of Freamine Ⅲ to oat root soil, stem, leaf even spraying,
In the watery stage it is sprayable more than twice, each interval time be not less than 10 days；In addition to aforesaid operations step, remaining operation can use
The prior art of wheat planting.
Crops first spread fertilizer over the fields base manure and carry out site preparation again before planting, and base manure uses bio-fertilizer, and chemical fertilizer is using survey soil
Formulation stage fertilizer practice, worm, the technology of sterilization or product, artificial weeding are controlled using biological pesticide and biology.The field of crops
Between management and watering routinely operation carry out.
Alkali cellulose enzyme, alkali protease, beer complex enzyme, mesophilicα-diastase, pectase belong to commercially available conventional production
Product, may be selected the Related product of Xia Sheng biotechnologies development corporation, Ltd., and beer complex enzyme contains 1,4 beta-glucanase, zytase
And enzyme activity >=8300u/ml of protease, wherein 1,4 beta-glucanase, enzyme activity >=2800u/ml of zytase, protease
Enzyme activity >=30000u/ml；The enzyme activity of alkali cellulose enzyme is 200,000 u/g, and the enzyme activity of alkali protease is 100,000 u/g；
The enzyme activity of pectase is 30,000 u/ml.Distiller's yeast used is to make every effort to overcome distiller's yeast.
Embodiment 1
The multienzyme kudzu root oral liquid of the present embodiment, is prepared using following methods：
1) artificial removal's impurity is coordinated with 20 mesh vibratory sieves after the whole strain of the root of kudzu vine (the multienzyme root of kudzu vine) harvesting, will with stalk crasher
It is 40 mesh that the whole strain of the multienzyme root of kudzu vine, which is crushed to particle diameter, obtains kudzu-vine root powder；10kg kudzu-vine root powders, 10kg wheat flours are after ozone sterilization goes out poison
Add in fermentation tank, add 100kg and drink sterilized water mixing, obtain mixed liquor A；
2) mesophilicα-diastase 8ml is added into mixed liquor A, calcium chloride 0.4g, 30min is digested at 80 DEG C, is mixed
Close liquid B；
Mixed liquid B is cooled to 30 DEG C, the pH value that mixed liquid B is adjusted with sodium bicarbonate is 8, adds alkali cellulose enzyme 6g,
Alkali protease 2g, 4h is digested at 50 DEG C, obtains mixed liquor C；
Mixed liquor C is cooled to 35 DEG C, adds beer complex enzyme 20ml, distiller's yeast 26g, anaerobic fermentation 7 days, anaerobic fermentation
1st~3 day, air agitation is carried out per 12h, the 7th day to fermentation ends, air agitation is carried out per 24h, obtains zymotic fluid；
Pectase 20ml, stirring reaction 90min are added into zymotic fluid, filtering, produces oral liquid finished product.
Embodiment 2
The multienzyme kudzu root oral liquid of the present embodiment, is prepared using following methods：
1) artificial removal's impurity is coordinated with 20 mesh vibratory sieves after the whole strain of the root of kudzu vine (the multienzyme root of kudzu vine) harvesting, will with stalk crasher
It is 40 mesh that the whole strain of the multienzyme root of kudzu vine, which is crushed to particle diameter, obtains kudzu-vine root powder；15kg kudzu-vine root powders, 15kg wheat flours are after ozone sterilization goes out poison
Add in fermentation tank, add 100kg and drink sterilized water mixing, obtain mixed liquor A；
2) mesophilicα-diastase 6ml is added into mixed liquor A, calcium chloride 0.6g, 25min is digested at 70 DEG C, is mixed
Close liquid B；
Mixed liquid B is cooled to 35 DEG C, the pH value that mixed liquid B is adjusted with sodium bicarbonate is 10, adds alkali cellulose enzyme 9g,
Alkali protease 3g, 5h is digested at 40 DEG C, obtains mixed liquor C；
Mixed liquor C is cooled to 35 DEG C, adds beer complex enzyme 30ml, distiller's yeast 30g, anaerobic fermentation 7 days, anaerobic fermentation
1st~3 day, air agitation is carried out per 12h, the 7th day to fermentation ends, air agitation is carried out per 24h, obtains zymotic fluid；
Pectase 35ml, stirring reaction 150min are added into zymotic fluid, filtering, produces oral liquid finished product.
Embodiment 3
The multienzyme kudzu root oral liquid of the present embodiment, is prepared using following methods：
1) artificial removal's impurity is coordinated with 20 mesh vibratory sieves after the whole strain of the root of kudzu vine (the multienzyme root of kudzu vine) harvesting, will with stalk crasher
It is 40 mesh that the whole strain of the multienzyme root of kudzu vine, which is crushed to particle diameter, obtains kudzu-vine root powder；20kg kudzu-vine root powders, 20kg wheat flours are after ozone sterilization goes out poison
Add in fermentation tank, add 100kg and drink sterilized water mixing, obtain mixed liquor A；
2) mesophilicα-diastase 20ml is added into mixed liquor A, calcium chloride 0.8g, 30min is digested at 60 DEG C, is mixed
Close liquid B；
Mixed liquid B is cooled to 50 DEG C, the pH value that mixed liquid B is adjusted with sodium bicarbonate is 11, adds alkali cellulose enzyme
12g, alkali protease 4g, 4h is digested at 50 DEG C, obtains mixed liquor C；
Mixed liquor C is cooled to 40 DEG C, adds beer complex enzyme 40ml, distiller's yeast 54g, anaerobic fermentation 7 days, anaerobic fermentation
1st~3 day, air agitation is carried out per 12h, the 7th day to fermentation ends, air agitation is carried out per 24h, obtains zymotic fluid；
Pectase 40ml, stirring reaction 200min are added into zymotic fluid, filtering, produces oral liquid finished product.
Test example
This test example detects the functional enzyme content of the gained multienzyme alkalescence oral liquid of 1~embodiment of embodiment 3 and stored
Enzymatic activity after regular period keeps situation (condition of storage is room temperature), as a result as shown in Table 1 to Table 3.
The content and active holding situation of the functional enzyme of the multienzyme alkalescence oral liquid of the embodiment 1 of table 1
The content and active holding situation of the functional enzyme of the multienzyme alkalescence oral liquid of the embodiment 2 of table 2
The content and active holding situation of the functional enzyme of the multienzyme alkalescence oral liquid of the embodiment 3 of table 3
Above-mentioned preparation method provided by the invention, without thermophilic digestion or autoclaving process, medicinal to Puerarin etc.
While composition is effectively extracted, SOD enzymes in the root of kudzu vine, CAT enzymes, POD enzymes, the activity of GSH-PX enzymes, above-mentioned function are remained
Property enzyme by " root of kudzu vine → fermentation → human body " approach enter human body in, there is higher security and absorbability, another aspect,
This method remains the endogenous SOD enzymes of the root of kudzu vine, CAT enzymes, POD enzymes, the primitive environment of GSH-PX enzymes, at utmost eliminates energy
The interfering material for enough promoting above-mentioned functional enzyme to convert or combine, it is stable so as to be advantageous to the long period of activity of above-mentioned functional enzyme.
In the kudzu root oral liquid of the present invention, the activity of the enzyme such as CAT, POD, GSH-PX is maintained, and these enzymes constitute clearly
Except the working chain of internal harmful free radicals, SOD enzymes together constitute removing together with coenzyme such as CAT, POD, GSH-PX and are harmful to freely
The complete chain of base.This method improves the utilization rate of the root of kudzu vine on the whole, the overall efficacy of the root of kudzu vine is more highlighted, to body
Health-care effect is obvious.
Claims (10)
1. a kind of multienzyme kudzu root oral liquid, it is characterised in that be prepared by the method comprised the following steps：
1) kudzu-vine root powder is mixed with water, prepares mixed liquor A；
2) amylase and activator are added into mixed liquor A, 20min~30min is digested at 60~85 DEG C, obtains mixed liquid B；
3) pH value for adjusting mixed liquid B is 8~12, alkali cellulose enzyme and protease is added into mixed liquid B, at 40~50 DEG C
4~5h of lower enzymolysis, obtains mixed liquor C；
4) beer complex enzyme and distiller's yeast are added into mixed liquor C, anaerobic fermentation is carried out at 35~40 DEG C, obtains zymotic fluid；To
Pectase is added in zymotic fluid, stirring reaction, filters and produces.
2. multienzyme kudzu root oral liquid as claimed in claim 1, it is characterised in that in step 1), add rich in SOD during mixing
The mass ratio of cereal crops, the cereal crops and kudzu-vine root powder is 1:1.
3. multienzyme kudzu root oral liquid as claimed in claim 1, it is characterised in that the kudzu-vine root powder by the multienzyme root of kudzu vine through crushing and
Into the multienzyme root of kudzu vine is cultivated to obtain by following methods, and this method includes：After root of kudzu vine final singling, to root of kudzu vine root soil, root,
Stem, leaf site sprinkling amino acid fertilizer.
4. multienzyme kudzu root oral liquid as claimed in claim 1, it is characterised in that in step 1), the kudzu-vine root powder mixes with water
Mass ratio be (20~40)：100.
5. multienzyme kudzu root oral liquid as claimed in claim 1, it is characterised in that in step 2), the amylase be medium temperature α-
Amylase, the activator are calcium chloride；The addition that mesophilicα-diastase is corresponded to per kg kudzu-vine root powders is 0.25~0.6ml, right
The addition for answering calcium chloride is 0.01~0.03g.
6. multienzyme kudzu root oral liquid as claimed in claim 1, it is characterised in that in step 2), beer is corresponded to per kg kudzu-vine root powders
The addition of complex enzyme is 0.4~1ml, and the addition of corresponding distiller's yeast is 0.8~1.4g；The beer complex enzyme contains β-Portugal and gathered
Enzyme activity >=8300u/g of carbohydrase, zytase and protease, wherein 1,4 beta-glucanase, the enzyme activity of zytase >=
2800u/g, enzyme activity >=30000u/g of protease.
7. multienzyme kudzu root oral liquid as claimed in claim 1, it is characterised in that in step 2), the time of the anaerobic fermentation
For 6~8 days.
8. multienzyme kudzu root oral liquid as claimed in claim 1, it is characterised in that the 1st~3 day of anaerobic fermentation, per 10h~
14h carries out air agitation, and the 4th day to fermentation ends, air agitation is carried out per 24h.
9. multienzyme kudzu root oral liquid as claimed in claim 1, it is characterised in that in step 2), pectin is corresponded to per kg zymotic fluids
The addition of enzyme is 1~5ml, and the enzyme activity of the pectase is 30,000 u/ml.
10. a kind of preparation method of multienzyme kudzu root oral liquid, it is characterised in that comprise the following steps：
1) kudzu-vine root powder is mixed with water, prepares mixed liquor A；
2) amylase and activator are added into mixed liquor A, 20min~30min is digested at 60~85 DEG C, obtains mixed liquid B；
3) pH value for adjusting mixed liquid B is 8~12, alkali cellulose enzyme and protease is added into mixed liquid B, at 40~50 DEG C
4~5h of lower enzymolysis, obtains mixed liquor C；
4) beer complex enzyme and distiller's yeast are added into mixed liquor C, anaerobic fermentation is carried out at 35~40 DEG C, obtains zymotic fluid；To
Pectase is added in zymotic fluid, stirring reaction, filters and produces.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710237469 | 2017-04-12 | ||
| CN2017102374690 | 2017-04-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107536034A true CN107536034A (en) | 2018-01-05 |
Family
ID=60970737
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710685282.7A Pending CN107536034A (en) | 2017-04-12 | 2017-08-11 | A kind of multienzyme kudzu root oral liquid and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107536034A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109078042A (en) * | 2018-08-14 | 2018-12-25 | 齐鲁工业大学 | A kind of method that compound bio enzymatic hydrolysis extracts active constituent in pueraria lobata |
| CN110179095A (en) * | 2019-07-08 | 2019-08-30 | 黑龙江省科学院大庆分院 | A kind of preparation method of Chinese edestan fermentation sauce and its preparation method of Chinese edestan fermentation sauce powder |
| CN112931736A (en) * | 2021-03-08 | 2021-06-11 | 林路生物科技(黄石)有限公司 | Preparation method of kudzu root tagatose oral liquid |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105231168A (en) * | 2015-10-28 | 2016-01-13 | 青海尤尼克生物技术有限公司 | Chinese wolfberry enzyme and preparation method thereof |
| CN105961003A (en) * | 2016-05-30 | 2016-09-28 | 路平 | Planting method of multienzyme crops containing SOD, multienzyme solution containing SOD and preparation method of solution |
-
2017
- 2017-08-11 CN CN201710685282.7A patent/CN107536034A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105231168A (en) * | 2015-10-28 | 2016-01-13 | 青海尤尼克生物技术有限公司 | Chinese wolfberry enzyme and preparation method thereof |
| CN105961003A (en) * | 2016-05-30 | 2016-09-28 | 路平 | Planting method of multienzyme crops containing SOD, multienzyme solution containing SOD and preparation method of solution |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109078042A (en) * | 2018-08-14 | 2018-12-25 | 齐鲁工业大学 | A kind of method that compound bio enzymatic hydrolysis extracts active constituent in pueraria lobata |
| CN109078042B (en) * | 2018-08-14 | 2022-05-31 | 齐鲁工业大学 | Method for extracting active ingredients from radix puerariae through compound biological enzymolysis |
| CN110179095A (en) * | 2019-07-08 | 2019-08-30 | 黑龙江省科学院大庆分院 | A kind of preparation method of Chinese edestan fermentation sauce and its preparation method of Chinese edestan fermentation sauce powder |
| CN112931736A (en) * | 2021-03-08 | 2021-06-11 | 林路生物科技(黄石)有限公司 | Preparation method of kudzu root tagatose oral liquid |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103766114B (en) | Selenium-rich fragrant taro cultivating method | |
| CN105103924B (en) | A kind of high yield cultivating method of the nuisanceless bletilla striata | |
| CN106416492B (en) | The implantation methods of high-yield rice | |
| CN107567757A (en) | A kind of breeding method of selenium-rich rice | |
| CN106348986A (en) | Dedicated tomato organic fertilizer and preparation method thereof | |
| CN106747933A (en) | A kind of Biological organic fertilizer special for cucumber and preparation method thereof | |
| CN106576546A (en) | Plantation method of selenium-rich edible canna | |
| CN106718459A (en) | A kind of organic paddy rice implantation methods | |
| CN105850410A (en) | Planting method for rice bean | |
| CN106550757A (en) | The high-yield planting method of rich strontium drought Rhizoma Nelumbinis | |
| CN106588467A (en) | Biological fertilizer special for radix sophorae subprostratae and preparation method thereof | |
| CN101935260A (en) | Plant-derived growth-promoting organic medical fertilizer and preparation method thereof | |
| CN106986691A (en) | A kind of cultural method of spun gold emperor chrysanthemum | |
| CN103102217A (en) | Soil-free plug seedling substrate and preparation method thereof | |
| CN102948324A (en) | Method for producing edible mushroom by used biological packing | |
| CN106577097A (en) | Citrus planting method | |
| CN107536034A (en) | A kind of multienzyme kudzu root oral liquid and preparation method thereof | |
| CN106946603A (en) | A kind of oil tree peony fertilizer special for organic and preparation method thereof | |
| CN106831202A (en) | A kind of potato special fertilizer and its preparation and application process | |
| CN107125017A (en) | A kind of breeding method of mushroom | |
| CN107853094A (en) | A kind of implantation methods of Semen sesami nigrum | |
| CN108207520A (en) | A kind of organic rice and its implantation methods | |
| CN104642030A (en) | High-yield planting method of organic rice | |
| CN105255744B (en) | A kind of lucidum strain purification and rejuvenation cultural method and its culture medium | |
| CN107500995A (en) | Effectively prevention garlic sits Eco-fertilizer of the root and stem of certain plants and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180105 |