CN106010974A - Pleurotus ferulae liquid strain and preparing method thereof - Google Patents

Pleurotus ferulae liquid strain and preparing method thereof Download PDF

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Publication number
CN106010974A
CN106010974A CN201610313806.5A CN201610313806A CN106010974A CN 106010974 A CN106010974 A CN 106010974A CN 201610313806 A CN201610313806 A CN 201610313806A CN 106010974 A CN106010974 A CN 106010974A
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bean milk
pleurotus ferulae
slagging
semen glycines
ferulae lanzi
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侯绪营
王小印
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WULUMUQI RESEARCH INSTITUTE OF EDIBLE MUSHROOMS
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WULUMUQI RESEARCH INSTITUTE OF EDIBLE MUSHROOMS
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor

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Abstract

The invention relates to the technical field of pleurotus ferulae, in particular to a pleurotus ferulae liquid strain and a preparing method thereof. The pleurotus ferulae liquid strain is prepared from, by weight, 0.4-0.6 part of soybean milk with residues removed, 0.4-1 part of corn starch, 1.5-2 parts of glucose and 0-0.01 part of maltodextrin. Compared with the prior art, the pleurotus ferulae liquid strain has the advantages that clump count is increased greatly, and clump count can still reach 800-1600 in each litre of supernatant liquid obtained after clarification of the pleurotus ferulae liquid strain; meanwhile, the number of germination points is large in each litre of pleurotus ferulae liquid strain, and germination period is shortened greatly by 1-2 days. The pleurotus ferulae liquid strain is suitable for industrial production, culture bag inoculation can be conducted directly for fruiting, volume production of fungus sacks can be conducted, period is shortened, labor is saved, and favorable conditions are created for intensive and standard edible fungus production.

Description

Pleurotus ferulae Lanzi liquid spawn and preparation method thereof
Technical field
The present invention relates to Pleurotus ferulae Lanzi technical field, be a kind of Pleurotus ferulae Lanzi liquid spawn and preparation method thereof.
Background technology
Pleurotus ferulae Lanzi has another name called Pleurotus ferulae Lanzi, asafoetida mushroom, belongs to Basidiomycotina Hymenomycetes Agaricales Pleurotaceae pleurotus, is mushroom fungus representative on aridity grass land.Owing to its sporophore is tender and crisp good to eat, aromatic flavor, there is the laudatory title of grassland bolete；There is again the drug effects such as removing food stagnancy, parasite killing, treatment meat long-pending, mass in the abdomen, chronic malaria, infantile malnutrition labor because of it, local masses' reputation for the white Ganoderma of Tianshan Mountains god mushroom and Western Paradise.Later owing to excessively plucking and domestic animal trample, natural resources is reduced by heavy damage, wild Pleurotus ferulae Lanzi year by year.Wild Pleurotus ferulae Lanzi is distributed mainly on the Yi Li of Desert Regions, basin, Gaer, Xinjiang, Tacheng and Altay Prefecture, and wherein the Pleurotus ferulae Lanzi popularity of Qinghe County, Altay Prefecture is the highest.Because of wild Pleurotus ferulae Lanzi only originate in particular season, yield is few, harvesting is difficult, Pleurotus ferulae Lanzi the most on the market is based on artificial culture.
The production technology of solid spawn, is usually and is become two grades of kinds, three grades of kinds by test tube stock expanding propagation, and production cycle length, pollution rate are high, cost is high, it is artificial in a large number to need and place, and difficult management；Liquid spawn produces has that purity is high, energetic, breed fast feature；But existing Pleurotus ferulae Lanzi liquid spawn exists, and poor fluidity, germination point be few, production cycle length and clump count are difficult to adapt to large-scale production needs less.
Summary of the invention
The invention provides a kind of Pleurotus ferulae Lanzi liquid spawn and preparation method thereof, overcoming the deficiency of above-mentioned prior art, it can effectively solve the problem that existing Pleurotus ferulae Lanzi liquid spawn exists that poor fluidity, germination point be few, production cycle length and clump count are difficult to adapt to large-scale production needs less.
One of technical scheme is realized by following measures: a kind of Pleurotus ferulae Lanzi liquid spawn, and raw materials by weight portion contains Semen Glycines slagging-off bean milk 0.4 Part is extremely 0.6 Part, corn starch 0.4 Part is extremely 1 Part, glucose 1.5 Part is extremely 2 Part, maltodextrin 0 Part is extremely 0.01 Part.
Be presented herein below to one of foregoing invention technical scheme optimize further or / And improvement:
Above-mentioned Pleurotus ferulae Lanzi liquid spawn obtains as follows: the first step, adds the desired amount of corn starch, glucose and maltodextrin mix homogeneously, obtain mixing liquid in the desired amount of Semen Glycines slagging-off bean milk；Second step, mixing liquid is heated to 90 DEG C extremely 95 After DEG C, the mixing liquid after heating is joined in fermentation tank, at pressure is 0.15MPa Lower sterilizing 40min ；3rd step, starts after being cooled to room temperature to inoculate Pleurotus ferulae Lanzi original seed, after inoculation in temperature be after sterilizing 23 DEG C extremely 25 Cultivate at DEG C 3d Extremely 5d After, obtain Pleurotus ferulae Lanzi liquid spawn.
Above-mentioned Semen Glycines slagging-off bean milk obtains as follows: takes appropriate Semen Glycines and soaks in water, and the Semen Glycines after soaking adds in slagging-off soy bean milk making machine, then adds water in slagging-off soy bean milk making machine and breaks into bean milk, obtains Semen Glycines slagging-off bean milk.
Above-mentioned Semen Glycines is soaked in water 8h Extremely 12h ；Slagging-off soy bean milk making machine adds 8 The most extremely 15 The water of times Semen Glycines quality breaks into bean milk.
The two of technical scheme are realized by following measures: the preparation method of a kind of Pleurotus ferulae Lanzi liquid spawn, carry out in the steps below: the first step, in the desired amount of Semen Glycines slagging-off bean milk, add the desired amount of corn starch, glucose and maltodextrin mix homogeneously, obtain mixing liquid；Second step, mixing liquid is heated to 90 DEG C extremely 95 After DEG C, the mixing liquid after heating is joined in fermentation tank, at pressure is 0.15MPa Lower sterilizing 40min ；3rd step, starts after being cooled to room temperature to inoculate Pleurotus ferulae Lanzi original seed, after inoculation in temperature be after sterilizing 23 DEG C extremely 25 Cultivate at DEG C 3d Extremely 5d After, obtain Pleurotus ferulae Lanzi liquid spawn.
Be presented herein below to foregoing invention technical scheme two optimize further or / And improvement:
Above-mentioned Semen Glycines slagging-off bean milk obtains as follows: takes appropriate Semen Glycines and soaks in water, and the Semen Glycines after soaking adds in slagging-off soy bean milk making machine, then adds water in slagging-off soy bean milk making machine and breaks into bean milk, obtains Semen Glycines slagging-off bean milk.
Above-mentioned Semen Glycines is soaked in water 8h Extremely 12h ；Slagging-off soy bean milk making machine adds 8 The most extremely 15 The water of times Semen Glycines quality breaks into bean milk.
The present invention has a very significant increase compared with the clump count of prior art Pleurotus ferulae Lanzi liquid spawn, and still contains in every liter of supernatant liquid after Pleurotus ferulae Lanzi liquid spawn of the present invention clarification 800 Individual extremely 1600 Individual clump count；The present invention every liter Pleurotus ferulae Lanzi liquid spawn germination point is many simultaneously, and sprouting cycle relatively prior art shortens 1d Extremely 2d , thus substantially reducing the sprouting cycle, Pleurotus ferulae Lanzi liquid spawn of the present invention is suitable for industrialized production, can carry out fruiting by direct inoculation cultivating bag, produce bacterium bag in enormous quantities, shorten the cycle, save artificial, create condition for edible fungi intensive, standardized production.
Detailed description of the invention
The present invention is not limited by following embodiment, can determine specific embodiment according to technical scheme and practical situation.
Embodiment 1 , this Pleurotus ferulae Lanzi liquid spawn raw materials by weight portion contains Semen Glycines slagging-off bean milk 0.4 Part is extremely 0.6 Part, corn starch 0.4 Part is extremely 1 Part, glucose 1.5 Part is extremely 2 Part, maltodextrin 0 Part is extremely 0.01 Part.
Embodiment 2 , this Pleurotus ferulae Lanzi liquid spawn raw materials by weight portion contains Semen Glycines slagging-off bean milk 0.4 Part or 0.6 Part, corn starch 0.4 Part or 1 Part, glucose 1.5 Part or 2 Part, maltodextrin 0 Part or 0.01 Part.
Embodiment 3 , this Pleurotus ferulae Lanzi liquid spawn is obtained by following preparation method: the first step, adds the desired amount of corn starch, glucose and maltodextrin mix homogeneously, obtain mixing liquid in the desired amount of Semen Glycines slagging-off bean milk；Second step, mixing liquid is heated to 90 DEG C extremely 95 After DEG C, the mixing liquid after heating is joined in fermentation tank, at pressure is 0.15MPa Lower sterilizing 40min ；3rd step, starts after being cooled to room temperature to inoculate Pleurotus ferulae Lanzi original seed, after inoculation in temperature be after sterilizing 23 DEG C extremely 25 Cultivate at DEG C 3d Extremely 5d After, obtain Pleurotus ferulae Lanzi liquid spawn.
Embodiment 4 , as the optimization of above-described embodiment, Semen Glycines slagging-off bean milk obtains as follows: takes appropriate Semen Glycines and soaks in water, and the Semen Glycines after soaking adds in slagging-off soy bean milk making machine, then adds water in slagging-off soy bean milk making machine and breaks into bean milk, obtains Semen Glycines slagging-off bean milk.
Embodiment 5 , as the optimization of above-described embodiment, Semen Glycines is soaked in water 8h Extremely 12h ；Slagging-off soy bean milk making machine adds 8 The most extremely 15 The water of times Semen Glycines quality breaks into bean milk.
Above-described embodiment 1 To embodiment 5 In the every liter of Pleurotus ferulae Lanzi liquid spawn obtained, clump count can reach 5000 Individual extremely 8000 Individual；Embodiment 1 To embodiment 5 The Pleurotus ferulae Lanzi liquid spawn clarification obtained 1h Extremely 2h After, every liter of supernatant liquid contains 800 Individual bacterium colony is extremely 1600 Individual bacterium colony；Embodiment simultaneously 1 To embodiment 5 The every liter of Pleurotus ferulae Lanzi liquid spawn obtained is strong because of molecule small flow, so germination point is many, thus substantially reduces the sprouting cycle.
Embodiment 6 , this Pleurotus ferulae Lanzi liquid spawn is obtained by following preparation method: the first step, adds the desired amount of corn starch and glucose mix homogeneously, obtain mixing liquid in the desired amount of Semen Glycines slagging-off bean milk；Second step, mixing liquid is heated to 90 After DEG C, the mixing liquid after heating is joined in fermentation tank, at pressure is 0.15MPa Lower sterilizing 40min ；3rd step, starts after being cooled to room temperature to inoculate Pleurotus ferulae Lanzi original seed, after inoculation in temperature be after sterilizing 23 Cultivate at DEG C 3d After, obtain Pleurotus ferulae Lanzi liquid spawn；Semen Glycines slagging-off bean milk obtains as follows: takes appropriate Semen Glycines and soaks in water 9h , the Semen Glycines after soaking adds in slagging-off soy bean milk making machine, then adds in slagging-off soy bean milk making machine 10 The water of times Semen Glycines quality breaks into bean milk, obtains Semen Glycines slagging-off bean milk；Wherein raw materials by weight portion contains Semen Glycines slagging-off bean milk 0.4 Part, corn starch 0.5 Part, glucose 1.5 Part.Embodiment 6 In the every liter of Pleurotus ferulae Lanzi liquid spawn obtained, clump count can reach 5020 Individual；Embodiment 6 The Pleurotus ferulae Lanzi liquid spawn clarification obtained 1h After, every liter of supernatant liquid contains 900 Individual bacterium colony；Embodiment simultaneously 6 The every liter of Pleurotus ferulae Lanzi liquid spawn obtained is strong because of molecule small flow, so germination point is many, thus substantially reduces the sprouting cycle.
Embodiment 7 , this Pleurotus ferulae Lanzi liquid spawn is obtained by following preparation method: the first step, adds the desired amount of corn starch, glucose and maltodextrin mix homogeneously, obtain mixing liquid in the desired amount of Semen Glycines slagging-off bean milk；Second step, mixing liquid is heated to 92 After DEG C, the mixing liquid after heating is joined in fermentation tank, at pressure is 0.15MPa Lower sterilizing 40min ；3rd step, starts after being cooled to room temperature to inoculate Pleurotus ferulae Lanzi original seed, after inoculation in temperature be after sterilizing 24 Cultivate at DEG C 3.5d After, obtain Pleurotus ferulae Lanzi liquid spawn；Semen Glycines slagging-off bean milk obtains as follows: takes appropriate Semen Glycines and soaks in water 11h , the Semen Glycines after soaking adds in slagging-off soy bean milk making machine, then adds in slagging-off soy bean milk making machine 12 The water of times Semen Glycines quality breaks into bean milk, obtains Semen Glycines slagging-off bean milk；Wherein raw materials by weight portion contains Semen Glycines slagging-off bean milk 0.5 Part, corn starch 0.7 Part, glucose 1.6 Part, maltodextrin 0.005 Part.Embodiment 7 In the every liter of Pleurotus ferulae Lanzi liquid spawn obtained, clump count can reach 7000 Individual；Embodiment 7 The Pleurotus ferulae Lanzi liquid spawn clarification obtained 1.5h After, every liter of supernatant liquid contains 1300 Individual bacterium colony；Embodiment simultaneously 7 The every liter of Pleurotus ferulae Lanzi liquid spawn obtained is strong because of molecule small flow, so germination point is many, thus substantially reduces the sprouting cycle.
Embodiment 8 , this Pleurotus ferulae Lanzi liquid spawn is obtained by following preparation method: the first step, adds the desired amount of corn starch, glucose and maltodextrin mix homogeneously, obtain mixing liquid in the desired amount of Semen Glycines slagging-off bean milk；Second step, mixing liquid is heated to 95 After DEG C, the mixing liquid after heating is joined in fermentation tank, at pressure is 0.15MPa Lower sterilizing 40min ；3rd step, starts after being cooled to room temperature to inoculate Pleurotus ferulae Lanzi original seed, after inoculation in temperature be after sterilizing 25 Cultivate at DEG C 4d After, obtain Pleurotus ferulae Lanzi liquid spawn；Semen Glycines slagging-off bean milk obtains as follows: takes appropriate Semen Glycines and soaks in water 11h , the Semen Glycines after soaking adds in slagging-off soy bean milk making machine, then adds in slagging-off soy bean milk making machine 14 The water of times Semen Glycines quality breaks into bean milk, obtains Semen Glycines slagging-off bean milk；Wherein raw materials by weight portion contains Semen Glycines slagging-off bean milk 0.6 Part, corn starch 0.8 Part, glucose 1.9 Part, maltodextrin 0.01 Part.Embodiment 8 In the every liter of Pleurotus ferulae Lanzi liquid spawn obtained, clump count can reach 8000 Individual；Embodiment 8 The Pleurotus ferulae Lanzi liquid spawn clarification obtained 2h After, every liter of supernatant liquid contains 1500 Individual bacterium colony；Embodiment simultaneously 8 The every liter of Pleurotus ferulae Lanzi liquid spawn obtained is strong because of molecule small flow, so germination point is many, thus substantially reduces the sprouting cycle.
The clump count of existing Pleurotus ferulae Lanzi liquid spawn averagely reaches 2000 Individual, clarification 1h Extremely 2h After, supernatant liquid does not has bacterium colony；In the present invention every liter Pleurotus ferulae Lanzi liquid spawn, clump count can reach 5000 Individual extremely 8000 Individual, clarification 1h Extremely 2h After, every liter of supernatant liquid contains 800 Individual bacterium colony is extremely 1600 Individual bacterium colony；Simultaneously the present invention every liter Pleurotus ferulae Lanzi liquid spawn because of molecule small flow strong, so germination point is many, sprouting cycle relatively prior art shortens 1d Extremely 2d , thus substantially reducing the sprouting cycle, Pleurotus ferulae Lanzi liquid spawn of the present invention is suitable for industrialized production, can carry out fruiting by direct inoculation cultivating bag, produce bacterium bag in enormous quantities, create condition for edible fungi intensive, standardized production.
In sum, the present invention has a very significant increase compared with the clump count of prior art Pleurotus ferulae Lanzi liquid spawn, and still contains in every liter of supernatant liquid after Pleurotus ferulae Lanzi liquid spawn of the present invention clarification 800 Individual extremely 1600 Individual clump count；The present invention every liter Pleurotus ferulae Lanzi liquid spawn germination point is many simultaneously, and sprouting cycle relatively prior art shortens 1d Extremely 2d , thus substantially reducing the sprouting cycle, Pleurotus ferulae Lanzi liquid spawn of the present invention is suitable for industrialized production, can carry out fruiting by direct inoculation cultivating bag, produce bacterium bag in enormous quantities, shorten the cycle, save artificial, create condition for edible fungi intensive, standardized production.
Above technical characteristic constitutes embodiments of the invention, and it has stronger adaptability and implementation result, can increase and decrease non-essential technical characteristic according to actual needs, meet the demand of different situations.

Claims (7)

1. a Pleurotus ferulae Lanzi liquid spawn, it is characterised in that raw materials by weight portion contains Semen Glycines slagging-off bean milk 0.4 part to 0.6 part, corn starch 0.4 part to 1 part, glucose 1.5 parts to 2 parts, maltodextrin 0 part to 0.01 part.
Pleurotus ferulae Lanzi liquid spawn the most according to claim 1, it is characterised in that obtain as follows: the first step, adds the desired amount of corn starch, glucose and maltodextrin mix homogeneously in the desired amount of Semen Glycines slagging-off bean milk, obtains mixing liquid；Second step, after mixing liquid is heated to 90 DEG C to 95 DEG C, joins in fermentation tank by the mixing liquid after heating, sterilizing 40min under pressure is 0.15MPa；3rd step, starts after being cooled to room temperature to inoculate Pleurotus ferulae Lanzi original seed after sterilizing, after cultivating 3d to 5d, obtains Pleurotus ferulae Lanzi liquid spawn after inoculation at temperature is 23 DEG C to 25 DEG C.
Pleurotus ferulae Lanzi liquid spawn the most according to claim 1 and 2, it is characterized in that Semen Glycines slagging-off bean milk obtains as follows: take appropriate Semen Glycines and soak in water, Semen Glycines after soaking adds in slagging-off soy bean milk making machine, then adds water in slagging-off soy bean milk making machine and breaks into bean milk, obtains Semen Glycines slagging-off bean milk.
Pleurotus ferulae Lanzi liquid spawn the most according to claim 3, it is characterised in that Semen Glycines soaks 8h to 12h in water；In slagging-off soy bean milk making machine, add 8 times of water to 15 times of Semen Glycines quality break into bean milk.
5. the preparation method of a Pleurotus ferulae Lanzi liquid spawn according to claim 1, it is characterized in that carrying out in the steps below: the first step, in the desired amount of Semen Glycines slagging-off bean milk, add the desired amount of corn starch, glucose and maltodextrin mix homogeneously, obtain mixing liquid；Second step, after mixing liquid is heated to 90 DEG C to 95 DEG C, joins in fermentation tank by the mixing liquid after heating, sterilizing 40min under pressure is 0.15MPa；3rd step, starts after being cooled to room temperature to inoculate Pleurotus ferulae Lanzi original seed after sterilizing, after cultivating 3d to 5d, obtains Pleurotus ferulae Lanzi liquid spawn after inoculation at temperature is 23 DEG C to 25 DEG C.
The preparation method of Pleurotus ferulae Lanzi liquid spawn the most according to claim 5, it is characterized in that Semen Glycines slagging-off bean milk obtains as follows: take appropriate Semen Glycines and soak in water, Semen Glycines after soaking adds in slagging-off soy bean milk making machine, then in slagging-off soy bean milk making machine, add water and break into bean milk, obtain Semen Glycines slagging-off bean milk.
The preparation method of Pleurotus ferulae Lanzi liquid spawn the most according to claim 3, it is characterised in that Semen Glycines soaks 8h to 12h in water；In slagging-off soy bean milk making machine, add 8 times of water to 15 times of Semen Glycines quality break into bean milk.
CN201610313806.5A 2016-05-12 2016-05-12 Pleurotus ferulae liquid strain and preparing method thereof Pending CN106010974A (en)

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CN106613351A (en) * 2016-12-26 2017-05-10 新疆农业科学院植物保护研究所 Method for field inoculation of Ferula asafetida plant with pleuratus ferulae liquid strain

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CN106613351A (en) * 2016-12-26 2017-05-10 新疆农业科学院植物保护研究所 Method for field inoculation of Ferula asafetida plant with pleuratus ferulae liquid strain
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Application publication date: 20161012