CN106577253A - Cultivating method for high-starch high-yield sweet potatoes - Google Patents

Cultivating method for high-starch high-yield sweet potatoes Download PDF

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CN106577253A
CN106577253A CN201611024123.4A CN201611024123A CN106577253A CN 106577253 A CN106577253 A CN 106577253A CN 201611024123 A CN201611024123 A CN 201611024123A CN 106577253 A CN106577253 A CN 106577253A
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potato
hybridized
pollination
hybridization
business
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CN106577253B (en
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唐升忠
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Yunnan tangboshu Biotechnology Co., Ltd
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Wenshan Golden State Potato Crops Research Institute Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility

Abstract

The invention belongs to the field of agricultural cultivation and particularly relates to a cultivating method for high-starch high-yield sweet potatoes. The cultivating method comprises the following steps of S1, parental selection, S2, induced flowering, S3, hybridization, S4, selfing, S5, secondary hybridization, S6, the third time of hybridization, S7, the fourth time of hybridization and S8, seeding. The novel variety of sweet potatoes cultivated through the cultivating method improves the starch content by 34.75%, the yield is improved by 40.14%, economic benefit of growers is increased, and the utilization rate of sweet potato industry processing is increased.

Description

A kind of high starch, the breeding method of high yield Radix Ipomoeae
Technical field
The invention belongs to field of agricultural cultivation, and in particular to a kind of high starch, the breeding method of high yield Radix Ipomoeae.
Background technology
Radix Ipomoeae (Ipomoea batatas (L) Lam.) belongs to tubular flower mesh, Convolvulaceae annual herb plant, long 2 meters with On, on prostration ground is oblique, blade is usually width egg shape, long 4-13 centimetre, wide 3-13 centimetre, corolla pink, white, lavender Or purple, mitriform or funnel-form, long 3-4 centimetre, capsule is avette or oblate, has false dissepiment, is divided into Room 4, has underground tuber, block Root spindle, crust khaki or aubergine.Radix Ipomoeae rich in proteins, starch, pectin, cellulose, aminoacid, vitamin and many Mineral is planted, has the reputation of " life prolonging food ".Sugar content reaches 15%-20%.There are anticancer, cardioprotection, prevention emphysema, glycosuria The effects such as disease, fat-reducing.Ming Dynasty's Li Shizhen《Compendium of Materia Medica》Note has " Rhizoma Dioscoreae esculentae qi-restoratives, spleen benefiting and stimulating the appetite, kidney tonifying are cloudy ", and says marine people The longevity of food.The traditional Chinese medical science regards Radix Ipomoeae as good medicine.
Traditional Radix Ipomoeae nursery is to adopt vegetative propagation technique, the kind for carrying out year after year using the tendril grown in potato wedges Plant, yield poorly, content of starch is low, variety deterioration is serious, pest and disease damage is more, poor quality, and utilization rate, commodity conversion rate be not high, mostly Plant for raise livestock in Around the house.Radix Ipomoeae tissue cultured seedling (detoxification Ipomoea batatas Seedling) can only play a part of to recover original seed, also not It is the cultivation and breed improvement of new varieties, though there is certain effect of increasing production, is not also the direct utilization of selection cross new varieties.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of high starch, the breeding method of high yield Radix Ipomoeae, to solve Existing Ipomoea batatas kind yields poorly, content of starch is not high and the problem of quality deterioration.
In order to solve above-mentioned technical problem, the technical scheme is that:
A kind of high starch, the breeding method of high yield Radix Ipomoeae, comprise the steps:
S1, Juvenile stage:Imperial potato 9, business's potato 19, golden potato 2 and purple cloud red heart potato is selected to be parent;
S2, induced flowering:Phytosaur potato 9, golden potato 2 and purple cloud red heart potato are planted respectively with cuttage;In August, by imperial potato 9 Number, the growing environment of golden potato 2 and purple cloud red heart potato be adjusted to light application time for 6~8h/d, intensity of illumination is 18000~ 50000Lx, temperature are 24~28 DEG C;Business's potato growing environment of No. 19 is adjusted to into light application time for 9~10h/d, intensity of illumination For 10000~30000Lx, temperature is 20~25 DEG C;And imperial potato 9, business's potato 19, golden potato 2 and purple cloud red heart potato are sprayed Foliage fertilizer;
S3, hybridization:Hybridized with No. 9 ♀ of imperial potato × ♂ of business's potato 19, the first filial generation A for obtaining;With No. 19 ♀ × dragons of business's potato No. 9 ♂ of potato are hybridized, the first filial generation B for obtaining;Hybridized with No. 2 ♀ of golden potato × purple cloud red heart potato ♂, the first filial generation C for obtaining; Hybridized with purple cloud red heart potato ♀ × ♂ of gold potato 2, the first filial generation D for obtaining;
Pollination in crossover operation adopts artificial pollination, after artificial pollination growing environment temperature is adjusted to 20~25 DEG C;
S4, selfing:Planted with the seed of the seed and B of A respectively, induced flowering, it is numerous so that A ♀ × A ♂ carry out selfing Grow, obtain second filial E, pollination adopts artificial pollination;Self propagated is carried out with B ♀ × B ♂, second filial F is obtained, pollination is adopted and manually awarded Powder;
S5, secondary hybridization:It is parent with E and F, induced flowering is hybridized with E ♀ × F ♂, obtains F3 G, and pollination is adopted Use artificial pollination;
S6, three hybridization:It is parent with G, C and D, induced flowering is hybridized with G ♀ × C ♂, obtains sub four generations H;With G ♀ × D ♂ are hybridized, and obtain sub four generations L;Pollination adopts artificial pollination;
S7, four hybridization:It is parent with H and L, induced flowering is hybridized with H ♀ × L ♂, obtains sub five generations M, and pollination is adopted Use artificial pollination;M is high starch, the red kind of high yield;
S8, sowing:The seed obtained in S7 is soaked 5~10 minutes in the mercuric chloride solution of 0.2% (m/v), Ran Hou Soak 10~15 minutes in 75% ethanol, seed is rinsed 3~5 times with clear water, seed is placed in 40~50 DEG C of warm water and is soaked Bubble 8~10 hours, then seed is layered in river sand carries out accelerating germination, after seed germination, program request is carried out, seedling is obtained;
Flower is isolated after artificial pollination.
In the high starch, the breeding method of high yield Radix Ipomoeae that the present invention is provided, it is preferable that the foliage fertilizer constituent Weight ratio be, boric acid:Dipotassium hydrogen phosphate:Water=2:5:60.
In the high starch, the breeding method of high yield Radix Ipomoeae that the present invention is provided, it is preferable that the side of described artificial pollination Method is removed male parent stamen with tweezers, then clamps male parent stamen with tweezers and smears on maternal stigma.
In the high starch, the breeding method of high yield Radix Ipomoeae that the present invention is provided, it is preferable that the induction in step S4 to S7 It is 6~8h/d that the condition bloomed is light application time, and intensity of illumination is 18000~45000Lx, and temperature is 24~28 DEG C.
In the high starch, the breeding method of high yield Radix Ipomoeae that the present invention is provided, it is preferable that flower is entered after artificial pollination The method of row isolation is that bagging is carried out to flower or corolla mouth is tied with line.
Above-mentioned technical proposal, due to repeatedly being hybridized using multiple kinds, so as to get new varieties content of starch improve 34.75%, output increased 40.14%, the economic benefit that improves Planting household is, the utilization that increased Radix Ipomoeae industrial processes Rate.
Specific embodiment
Below the specific embodiment of the present invention is described further.Here is it should be noted that for these enforcements The explanation of mode is used to help understand the present invention, but does not constitute limitation of the invention.Additionally, invention described below As long as involved technical characteristic does not constitute conflict each other and can just be mutually combined in each embodiment.
Embodiment
A kind of high starch, the breeding method of high yield Radix Ipomoeae, the cultivating process are carried out in warmhouse booth, concrete to wrap Include following steps:
S1, Juvenile stage:Imperial potato 9, business's potato 19, golden potato 2 and purple cloud red heart potato is selected to be parent;
S2, induced flowering:Phytosaur potato 9, golden potato 2 and purple cloud red heart potato are planted respectively with cuttage.In August, by imperial potato 9 Number, the growing environment of golden potato 2 and purple cloud red heart potato be adjusted to light application time for 6~8h/d, intensity of illumination is 18000~ 50000Lx, temperature are 24~28 DEG C;Business's potato growing environment of No. 19 is adjusted to into light application time for 9~10h/d, intensity of illumination For 10000~30000Lx, temperature is 20~25 DEG C;And while to imperial potato 9, business's potato 19, golden potato 2 and purple cloud red heart potato Spray foliage fertilizer;The weight ratio of the foliage fertilizer constituent is, boric acid:Dipotassium hydrogen phosphate:Water=2:5:60.
S3, hybridization:Hybridized with No. 9 ♀ of imperial potato × ♂ of business's potato 19, obtained first filial generation A;With No. 19 ♀ of business's potato × dragon potato No. 9 ♂ are hybridized, and obtain first filial generation B;Hybridized with No. 2 ♀ of golden potato × purple cloud red heart potato ♂, obtained first filial generation C;With purple cloud Red heart potato ♀ × ♂ of gold potato 2 is hybridized, and obtains first filial generation D;
Using artificial pollination, the method for artificial pollination is removed male parent stamen with tweezers, then clamps male parent with tweezers Stamen is smeared on maternal stigma.Growing environment temperature is adjusted to 20~25 DEG C after artificial pollination;
S4, selfing:Planted with the seed of the seed and B of A respectively, induced flowering, the method for artificial pollination is adopted with A ♀ × A ♂ carry out self propagated, obtain second filial E, carry out self propagated with B ♀ × B ♂, obtain second filial F;
S5, secondary hybridization:It is parent with E and F, induced flowering adopts the method for artificial pollination to carry out with E ♀ × F ♂ miscellaneous Hand over, obtain F3 G;
S6, three hybridization:It is parent with G, C and D, induced flowering adopts the method for artificial pollination to carry out with G ♀ × C ♂ miscellaneous Hand over, obtain sub four generations H;Hybridized with G ♀ × D ♂, obtained sub four generations L;
S7, four hybridization:It is parent with H and L, induced flowering adopts the method for artificial pollination to carry out with H ♀ × L ♂ miscellaneous Hand over, obtain sub five generations M, M is high starch, high yield Ipomoea batatas kind;
S8, sowing:The seed obtained in S7 is soaked 5~10 minutes in the mercuric chloride solution of 0.2% (m/v), Ran Hou Soak 10~15 minutes in 75% ethanol, seed is rinsed 3~5 times with clear water, seed is placed in 40~50 DEG C of warm water and is soaked Bubble 8~10 hours, then seed is layered in river sand carries out accelerating germination, after seed germination, program request is carried out, seedling is obtained;
Flower is isolated after artificial pollination in above-mentioned all steps, partition method can carry out bagging to flower, Can also tie corolla mouth with line..
In step S4 to S7, the condition of induced flowering is that light application time is 6~8h/d, intensity of illumination for induced flowering condition For 18000~45000Lx, temperature is 24~28 DEG C.
Test example
In Yunnan Wenshan Prefecture area, the Radix Ipomoeae cultivated by embodiment, imperial potato 9, purple cloud are planted respectively red within 2015 Heart potato, business's potato 19 and golden potato 2 are each 10 mu, investigate their per mu yield and content of starch, the results are shown in Table 1:
The yield and content of starch of 1 each kind Radix Ipomoeae of table
Test group Embodiment Imperial potato 9 Purple cloud red heart potato Business's potato 19 Golden potato 2
Yield (kg/ mus) 6082 4340 2300 4002 2800
Content of starch (%) 31.90 9.18 21.34 23.67 14.97
As can be seen from the above table, the variety yield of embodiment be 6082kg/ mus, content of starch be 31.90% both 5 Individual kind highest;The yield highest (being 4340kg/ mus) of imperial potato 9, the content of starch that business's potato 19 in remaining 4 kind Highest (being 24.67%);The kind of embodiment 40.14% relative to No. 9 output increaseds of imperial potato, relative to business's sweet potato starch content Improve 34.75%.The new varieties sweet potato starch content that the present invention is cultivated improves 34.75%, output increased 40.14%, The economic benefit that improve Planting household can be widely applied to eat Radix Ipomoeae market pin raw, the utilization rate that increased Radix Ipomoeae industrial processes Sell, food processing and industrial processes.
Above embodiments of the present invention are explained in detail, but the invention is not restricted to described embodiment.It is right For those skilled in the art, in the case of without departing from the principle of the invention and spirit, these embodiments are carried out many Plant change, modification, replace and modification, still fall within protection scope of the present invention.

Claims (5)

1. a kind of high starch, the breeding method of high yield Radix Ipomoeae, it is characterised in that comprise the steps:
S1, Juvenile stage:Imperial potato 9, business's potato 19, golden potato 2 and purple cloud red heart potato is selected to be parent;
S2, induced flowering:Phytosaur potato 9, golden potato 2 and purple cloud red heart potato are planted respectively with cuttage;In August, by imperial potato 9, The growing environment of golden potato 2 and purple cloud red heart potato is adjusted to light application time for 6~8h/d, and intensity of illumination is 18000~ 50000Lx, temperature are 24~28 DEG C;Business's potato growing environment of No. 19 is adjusted to into light application time for 9~10h/d, intensity of illumination For 10000~30000Lx, temperature is 20~25 DEG C;And imperial potato 9, business's potato 19, golden potato 2 and purple cloud red heart potato are sprayed Foliage fertilizer;
S3, hybridization:Hybridized with No. 9 ♀ of imperial potato × ♂ of business's potato 19, the first filial generation A for obtaining;With No. 19 ♀ of business's potato × dragon potato 9 Number ♂ is hybridized, and obtains first filial generation B;Hybridized with No. 2 ♀ of golden potato × purple cloud red heart potato ♂, obtained first filial generation C;It is red with purple cloud Heart potato ♀ × ♂ of gold potato 2 is hybridized, and obtains first filial generation D;
Hybridization adopts artificial pollination, after artificial pollination growing environment temperature is adjusted to 20~25 DEG C;
S4, selfing:A and B being planted respectively, induced flowering carries out self propagated with A ♀ × A ♂, obtaining second filial E, pollination adopts people Work is pollinated;Self propagated is carried out with B ♀ × B ♂, second filial F is obtained, pollination adopts artificial pollination;
S5, secondary hybridization:It is parent with E and F, induced flowering is hybridized with E ♀ × F ♂, obtains F3 G, pollination adopts people Work is pollinated;
S6, three hybridization:It is parent with G, C and D, induced flowering is hybridized with G ♀ × C ♂, obtains sub four generations H;With G ♀ × D ♂ Hybridized, obtained sub four generations L;Pollination adopts artificial pollination;
S7, four hybridization:It is parent with H and L, induced flowering is hybridized with H ♀ × L ♂, obtains sub five generations M, pollination adopts people Work is pollinated;M is high starch, high yield Ipomoea batatas kind;
S8, sowing:The seed obtained in S7 is soaked 5~10 minutes in the mercuric chloride solution of 0.2% (m/v), then 75% Soak 10~15 minutes in ethanol, seed rinsed 3~5 times with clear water, by seed be placed in 40~50 DEG C of warm water immersion 8~ 10 hours, then seed is layered in river sand carries out accelerating germination, after seed germination, program request is carried out, seedling is obtained;
Flower is isolated after artificial pollination.
2. high starch according to claim 1, the breeding method of high yield Radix Ipomoeae, it is characterised in that the foliage fertilizer group Weight ratio into composition is, boric acid:Dipotassium hydrogen phosphate:Water=2:5:60.
3. high starch according to claim 1, the breeding method of high yield Radix Ipomoeae, it is characterised in that described manually awards The method of powder is removed male parent stamen with tweezers, then clamps male parent stamen with tweezers and smears on maternal stigma.
4. high starch according to claim 1, the breeding method of high yield Radix Ipomoeae, it is characterised in that in step S4 to S7 Induced flowering condition be light application time be 6~8h/d, intensity of illumination be 18000~45000Lx, temperature be 24~28 DEG C.
5. high starch according to claim 1, the breeding method of high yield Radix Ipomoeae, it is characterised in that right after artificial pollination The method isolated by flower is that bagging is carried out to flower or corolla mouth is tied with line.
CN201611024123.4A 2016-11-15 2016-11-15 A kind of high starch, the breeding method of high yield sweet potato Active CN106577253B (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107114229A (en) * 2017-05-05 2017-09-01 漯河市农业科学院 A kind of rapidly and efficiently breeding method of the disease-resistant sweet potato variety of the high starch of breeding high-yield
CN107258531A (en) * 2017-07-31 2017-10-20 扬州大学 A kind of simple isolation method of eggplant florescence pollination

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001031018A1 (en) * 1999-10-29 2001-05-03 Korea Research Institute Of Bioscience And Biotechnology A peroxidase genomic gene derived from ipomoea batatas and a promoter thereof
JP2001278902A (en) * 2000-03-31 2001-10-10 National Agriculture Research Center Sweet potato starch to be gelatinized at low temperature and method of breeding sweet potato including the starch in tuberous root
CN102405826A (en) * 2011-08-02 2012-04-11 河北省农林科学院粮油作物研究所 Method for breeding inbred line by using sweet potato special materials

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001031018A1 (en) * 1999-10-29 2001-05-03 Korea Research Institute Of Bioscience And Biotechnology A peroxidase genomic gene derived from ipomoea batatas and a promoter thereof
JP2001278902A (en) * 2000-03-31 2001-10-10 National Agriculture Research Center Sweet potato starch to be gelatinized at low temperature and method of breeding sweet potato including the starch in tuberous root
CN102405826A (en) * 2011-08-02 2012-04-11 河北省农林科学院粮油作物研究所 Method for breeding inbred line by using sweet potato special materials

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107114229A (en) * 2017-05-05 2017-09-01 漯河市农业科学院 A kind of rapidly and efficiently breeding method of the disease-resistant sweet potato variety of the high starch of breeding high-yield
CN107258531A (en) * 2017-07-31 2017-10-20 扬州大学 A kind of simple isolation method of eggplant florescence pollination

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