CN102726210B - Method for preparing cultivars of artificially-domesticated red-soil termitomyces albuminosus - Google Patents
Method for preparing cultivars of artificially-domesticated red-soil termitomyces albuminosus Download PDFInfo
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- CN102726210B CN102726210B CN201210145904.4A CN201210145904A CN102726210B CN 102726210 B CN102726210 B CN 102726210B CN 201210145904 A CN201210145904 A CN 201210145904A CN 102726210 B CN102726210 B CN 102726210B
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Abstract
The invention discloses a method for preparing cultivars of artificially-domesticated red-soil termitomyces albuminosus, which is the practical application of an artificial domestication biotechnology, and solves the technical problem of artificial cultivation of wild fungi. The technical method comprises the following steps: (1) preparing a raw material of a cultivar culture-medium, and adding water into the raw material, so that the water content of the obtained product is 40-60%, wherein the raw material comprises the following components in part by weight: 50-90 parts of food grains, 8-48 parts of sawdust, one part of monopotassium phosphate, and one part of sugar; (2) preparing the raw material of the cultivar culture-medium; (3) preparing a feed of the cultivar culture medium; and (4) inoculating the feed to artificially-domesticated original strains of red-soil termitomyces albuminosus through sterile operation to carry out light-tight cultivation, and after 25-65 days, mycelium are overgrown in a culture flask and become mature stock seeds, carrying out propagation on the mature stock seeds so as to obtain the cultivars of red-soil termitomyces albuminosus. The cultivars of red-soil termitomyces albuminosus disclosed by the invention can be used for artificially planting and producing the red-soil termitomyces albuminosus.
Description
Technical field
The present invention discloses a kind of method continuing to prepare cultivated species after wild mushroom domestication obtains female kind, the preparation method of domestication's laterite collybia albuminosa cultivated species specifically.
Background technology
Laterite collybia albuminosa (
oudemansiella radicata var.
alba) be the distinctive wild-type strain in Yunnan Province of China province laterite plateau, fruit body is medium to large-scale, and only growth economizes the highlands of height above sea level more than 1800 meters in Yunnan Province of China.Usually grow summer and autumn on forest land moist in weakly acidic red soil, therefore named laterite chicken fir, low form mushroom in genus, wild output is extremely low, very precious.Its outward appearance is pleasant, delicious flavour, high nutritive value.Laterite collybia albuminosa mushroom body is large, stem slightly long, cap surperficial light brown, stem is white, mushroom lid is abundant, best in quality
.at present, both at home and abroad without any the precedent of domesticating and cultivating wild laterite collybia albuminosa fruit body.
Carry before stopping present patent application, what in China's patent application, relate to collybia albuminosa plantation does not go up 10, and all there is no mandate, and subject matter is that bacterial classification proves that dynamics is weak, and there is no preservation accreditation, its collybia albuminosa proposed is difficult to confirmation.
Because of the special nutrition of laterite collybia albuminosa, taste and health-care effect, wild mushroom product price is always very high, plucks wild laterite collybia albuminosa and has been difficult to the great demand meeting market merely.For this reason, be necessary very much to carry out artificial domesticating cultivation technological development.
The present invention makes wild laterite collybia albuminosa artificial domesticating cultivation step second step, carry out wild laterite collybia albuminosa domestication mother and plant the technological development continuing to cultivate cultivated species, and be achieved, for industrial technology basis has been established in the sustainable use of the precious wild mushroom resource in Yunnan Province.
Summary of the invention
Object of the present invention is just to provide the preparation method continuing to cultivate cultivated species after a kind of wild laterite collybia albuminosa domestication mother that reentries plants.
Realize technical scheme of the present invention and process is such: the preparation method of domestication's laterite collybia albuminosa cultivated species, use bacterial classification to plant for laterite collybia albuminosa domestication mother, procedure is,
First, preparation Cultivar culture medium raw material, its material and composition by weight be, grain 50 ~ 90 parts, wood chip 8 ~ 48 parts, potassium dihydrogen phosphate 1 part, sugared 1 part, be raw material, add water and make water content be 40% ~ 60%;
Secondly, preparation Cultivar culture medium raw material, process approach is for soaking grain 24 ~ 72 hours or boiling to grain without the white heart, add wood chip, potassium dihydrogen phosphate, sugar, water substance by the requirement of culture medium raw material component afterwards, stir rear citric acid adjust pH 5.0 ~ 6.5;
Then, Cultivar culture medium material is prepared, adopt tree branches or batten, can bamboo cane, soak 24 ~ 72 hours, or boil 1 ~ 3 hour, after cooling, pour the Cultivar culture medium raw material prepared into, stir rear citric acid adjust pH 5.0 ~ 6.5, then loaded in blake bottle or culture bag, at 120 ~ 130 DEG C, sterilizing 120 ~ 210 minutes, prepares Cultivar culture medium material after cooling;
Finally, lucifuge cultivation is carried out by sterile working access laterite collybia albuminosa domestication mother kind, cultivation temperature is 15 ~ 28 DEG C, cultivate 25 ~ 65 days mycelium and cover with blake bottle or culture bag, become ripe original seed, then carry out expansion numerous after namely obtain domestication's laterite collybia albuminosa cultivated species.
Wherein, prepare Cultivar culture medium raw material, its material and composition by weight also can be, with agricultural crop straw 40 ~ 70 parts, wood chip 50 ~ 20 parts, 8 parts, wheat bran, potassium dihydrogen phosphate 1 part, sugar 1 part, be raw material, add water and make water content be 60% ~ 68%;
Or be, the broad-leaved tree branch of long 3 ~ 35 centimetres, diameter 0.3 ~ 2 centimetre or long 3 ~ 35 centimetres, the batten of sectional area 0.1 ~ 4 square centimeter or bamboo cane 90 parts, fine rice bran or corn flour 6 parts, potassium dihydrogen phosphate 2 parts, sugar 2 parts is raw material, add water and make water content be 40% ~ 60%.
The grain used comprises buckwheat, wheat, barley, oat, rye, triticale, corn, Chinese sorghum, paddy rice, dryland rice, highland barley, millet, broom corn millet etc.
The agricultural crop straw used comprises straw, wheat straw, cornstalk, beans stalk, peanut stalk, Rape Straw, cotton stalk, bagasse and hemp stalk etc.
Beneficial effect of the present invention is: the original mother wild laterite collybia albuminosa domestication obtained first plants and continues to cultivate into cultivated species, for artificial cultivation laterite collybia albuminosa has stepped second step again; The cultivated species mycelial growth obtained rapidly, healthy and strong, look white, field planting is fast, strong stress resistance and fruiting morning, quality better, output are high.
Accompanying drawing explanation
The present invention is preparation method to the effect that, therefore provides without concrete accompanying drawing.
The laterite collybia albuminosa that the present invention relates to (
oudemansiella radicata var.alba) declared bioactive materials and preserve, self-defined Code Name is FBM-001, with on April 13rd, 2012, at China Committee for Culture Collection of Microorganisms's common micro-organisms center, be called for short CGMCC and carried out preservation, deposit number is CGMCC5970, and proves survival.
Embodiment
Below to preparation method of the present invention, provide some of the more specific embodiments described.
Embodiment 1: the preparation method of domestication's laterite collybia albuminosa cultivated species
Preparation method's process is:
First, preparation Cultivar culture medium raw material, its material and composition by weight be, grain 50 ~ 90 parts, wood chip 8 ~ 48 parts, potassium dihydrogen phosphate 1 part, sugared 1 part, be raw material, add water and make water content be 40% ~ 60%;
Secondly, preparation Cultivar culture medium raw material, process approach is for soaking grain 24 ~ 72 hours or boiling to grain without the white heart, add wood chip, potassium dihydrogen phosphate, sugar, water substance by the requirement of culture medium raw material component afterwards, stir rear citric acid adjust pH 5.0 ~ 6.5;
Then, Cultivar culture medium material is prepared, adopt tree branches, soak 24 ~ 72 hours, or boil 1 ~ 3 hour, after cooling, pour the Cultivar culture medium raw material prepared into, stir rear citric acid adjust pH 5.0 ~ 6.5, then loaded in blake bottle or culture bag, at 120 ~ 130 DEG C, sterilizing 120 ~ 210 minutes, prepares Cultivar culture medium material after cooling;
Finally, lucifuge cultivation is carried out by sterile working access laterite collybia albuminosa domestication mother kind, cultivation temperature is 15 ~ 28 DEG C, cultivate 25 ~ 65 days mycelium and cover with blake bottle or culture bag, become ripe original seed, then carry out expansion numerous after namely obtain domestication's laterite collybia albuminosa cultivated species.
Embodiment 2: the preparation method of domestication's laterite collybia albuminosa cultivated species
Preparation method's process is:
First, prepare Cultivar culture medium raw material, its material and composition by weight are, with agricultural crop straw 40 ~ 70 parts, wood chip 50 ~ 20 parts, 8 parts, wheat bran, potassium dihydrogen phosphate 1 part, sugar 1 part, are raw material, add water and make water content be 60% ~ 68%;
Secondly, preparation Cultivar culture medium raw material, process approach is by agricultural crop straw immersion 24 ~ 72 hours, adds wood chip, wheat bran, potassium dihydrogen phosphate, sugar, water substance by the requirement of culture medium raw material component afterwards, stirs rear citric acid adjust pH 5.0 ~ 6.5;
Then, Cultivar culture medium material is prepared, adopt bamboo cane, soak 24 ~ 72 hours, or boil 1 ~ 3 hour, after cooling, pour the Cultivar culture medium raw material prepared into, stir rear citric acid adjust pH 5.0 ~ 6.5, then loaded in blake bottle or culture bag, at 120 ~ 130 DEG C, sterilizing 120 ~ 210 minutes, prepares Cultivar culture medium material after cooling;
Finally, lucifuge cultivation is carried out by sterile working access laterite collybia albuminosa domestication mother kind, cultivation temperature is 15 ~ 28 DEG C, cultivate 25 ~ 65 days mycelium and cover with blake bottle or culture bag, become ripe original seed, then carry out expansion numerous after namely obtain domestication's laterite collybia albuminosa cultivated species.
Above-mentioned used raw material is all existing products, can choice for use.
Claims (2)
1. the preparation method of domestication's laterite collybia albuminosa cultivated species, uses bacterial classification for laterite collybia albuminosa domestication mother kind, it is characterized in that:
(1) prepare Cultivar culture medium raw material, its material and composition by weight be, grain 50 ~ 90 parts, wood chip 8 ~ 48 parts, potassium dihydrogen phosphate 1 part, sugared 1 part, be raw material, add water and make water content be 40% ~ 60%;
(2) prepare Cultivar culture medium raw material, process approach is for soaking grain 24 ~ 72 hours or boiling to grain without the white heart, add wood chip, potassium dihydrogen phosphate, sugar, water substance by the requirement of culture medium raw material component afterwards, stir rear citric acid and adjust pH value 5.0 ~ 6.5;
(3) prepare Cultivar culture medium material, tree branches is adopted to soak 24 ~ 72 hours, or boil 1 ~ 3 hour, after cooling, pour the Cultivar culture medium raw material prepared into, stir rear citric acid and adjust pH value 5.0 ~ 6.5, then loaded in blake bottle or culture bag, at 120 ~ 130 DEG C, sterilizing 120 ~ 210 minutes, prepares Cultivar culture medium material after cooling;
(4) plant by sterile working access laterite collybia albuminosa domestication mother and carry out lucifuge cultivation, cultivation temperature is 15 ~ 28 DEG C, cultivate 25 ~ 65 days mycelium and cover with blake bottle or culture bag, become ripe original seed, then carry out expansion numerous after namely obtain domestication's laterite collybia albuminosa cultivated species.
2. the preparation method of domestication's laterite collybia albuminosa cultivated species according to claim 1, it is characterized in that: preparation Cultivar culture medium raw material, its material and composition by weight also can be, with crop stalk 40 ~ 70 parts, wood chip 50 ~ 20 parts, 8 parts, wheat bran, potassium dihydrogen phosphate 1 part, sugar 1 part, for raw material, add water and make water content be 60% ~ 68%;
Or be, the broad-leaved tree branch of long 3 ~ 35 centimetres, diameter 0.3 ~ 2 centimetre or long 3 ~ 35 centimetres, the batten of sectional area 0.1 ~ 4 square centimeter or bamboo cane 90 parts, fine rice bran or corn flour 6 parts, potassium dihydrogen phosphate 2 parts, sugar 2 parts is raw material, add water and make water content be 40% ~ 60%.
3. the preparation method of domestication's laterite collybia albuminosa cultivated species according to claim 1, is characterized in that:
Grain comprises buckwheat, wheat, barley, oat, rye, triticale, corn, jowar, paddy rice, dryland rice, highland barley, millet, broom corn millet.
4. the preparation method of domestication's laterite collybia albuminosa cultivated species according to claim 1, is characterized in that: crop stalk comprises straw, wheat straw, cornstalk, beans stalk, peanut stalk, Rape Straw, cotton stalk, bagasse and hemp stalk.
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| CN201210145904.4A CN102726210B (en) | 2012-05-13 | 2012-05-13 | Method for preparing cultivars of artificially-domesticated red-soil termitomyces albuminosus |
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| CN201210145904.4A CN102726210B (en) | 2012-05-13 | 2012-05-13 | Method for preparing cultivars of artificially-domesticated red-soil termitomyces albuminosus |
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| CN102726210B true CN102726210B (en) | 2015-06-24 |
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| CN104365378A (en) * | 2014-11-12 | 2015-02-25 | 江苏菇本堂生物科技股份有限公司 | White beech mushroom liquid strain preparation method with sorghum husks as raw materials |
| CN105103937B (en) * | 2015-07-16 | 2018-09-07 | 漳州市农业科学研究所 | A method of making agaricus bisporus original seed using bitter buckwheat and bacteria residue |
| CN105474995A (en) * | 2015-12-21 | 2016-04-13 | 镇江盛弘景观植物有限公司 | Cultivation and domestication method of wild collybia albuminosa |
| CN105541439A (en) * | 2016-01-25 | 2016-05-04 | 赵建荣 | Edible fungus strain culture medium, application of edible fungus strain culture medium in black collybia albuminosa culture and culture method |
| CN106316654A (en) * | 2016-09-24 | 2017-01-11 | 云南福保农业科技开发有限公司 | Oudemansiella raphanipes liquid strain culture medium and preparation method thereof |
| CN107624502A (en) * | 2017-10-31 | 2018-01-26 | 云南天隼生物科技有限公司 | A kind of cultural method for manually cultivating grey chicken fir |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101218876A (en) * | 2007-12-26 | 2008-07-16 | 孙思国 | Termitomyces albuminosus with black skin producing process |
| CN102249792A (en) * | 2010-05-22 | 2011-11-23 | 周生建 | Method for preparing collybia albuminosa compost |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101218876A (en) * | 2007-12-26 | 2008-07-16 | 孙思国 | Termitomyces albuminosus with black skin producing process |
| CN102249792A (en) * | 2010-05-22 | 2011-11-23 | 周生建 | Method for preparing collybia albuminosa compost |
Non-Patent Citations (1)
| Title |
|---|
| 张玉金等.鸡枞的驯化栽培现状.《微生物学报》.2010,第50卷(第10期),1288-1292. * |
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