CN107624502A - A kind of cultural method for manually cultivating grey chicken fir - Google Patents
A kind of cultural method for manually cultivating grey chicken fir Download PDFInfo
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Abstract
The present invention is a kind of cultural method for manually cultivating grey chicken fir, step is prepared including one-level kind, prepared by two level kind, culture matrix preparation, the access of cultigen, mushroom producing culture；The collybia albuminosa cultivated by the inventive method, achievable whole year can produce chicken fir, make the production of collybia albuminosa not influenceed by season, and by the inventive method, the large-scale production of chicken fir can be achieved；The inventive method production has extraordinary bionical effect, and nutritive value is very high, and secondary mushroom can directly eat it raw, and has safe and non-toxic effect.
Description
Technical field
The present invention relates to planting edible mushroom technical field, a kind of specifically cultural method for manually cultivating grey chicken fir.
Background technology
Black collybia albuminosa Xerula pudens (Pers.:Fr.) sing is the famous wild mushroom in Yunnan, and its fructification is medium extremely
It is more small-sized.Most area in Yunnan is distributed.Summer and autumn is born on the thicker moist forest land of soil meta-acid, humus,
Grown out from the rotten timber in sylvan life soil, belong to high temperature type mushroom, wild yield is extremely low, very precious.Its outward appearance
Pleasant, delicious flavour, nutritive value is high, and rich in protein, fat, carbohydrate etc., its mushroom lid is abundant, tender and smooth,
Mushroom handle tubbiness, sweet tea is glutinous tasty, and whole mushroom is edible, is superior Rare edible fungus.No matter fresh mushroom or the equal good mouthfeel of dry product, to be first-class
Delicious food.There is data to show, the active factors of black chicken fir can increase pepsin activity, contribute to proteolytic digestion to inhale
Receive.The growth of helicobacter pylori can be suppressed, and play the role of to repair damaged gastric mucosa.Black chicken fir can also tranquilizing and allaying excitement, delay
Gastrointestinal convulsion is solved, improves the phagocytic activity of immunity and macrophage, can medicine-food two-purpose.
But chicken fir, due to belonging to wild mushrooms, its production is subject to seasonal restrictions, and can not meet the needs of long-term, existing skill
The problem of expanding mould contamination when cultivating, Er Qiepei be present more in the method that art discloses some artificial culture chicken firs, many methods
The bionical effect of chicken fir educated is poor, and nutritive value is not high, and its mouthfeel and wild chicken fir can not meet to disappear there is also very big difference
Take demand.
The content of the invention
The purpose of the present invention is the problem of presence for above-mentioned technology, there is provided a kind of culture side for manually cultivating grey chicken fir
Method.
To achieve the above object, the technical solution adopted by the present invention is：A kind of cultural method for manually cultivating grey chicken fir, bag
Include following steps：
(1) prepared by one-level kind：By potassium dihydrogen phosphate 0.2g, dipotassium hydrogen phosphate 0.2g, agar 20g, glucose 20g, formic acid
5g, gypsum 5g, magnesium sulfate 2g, yeast extract 5g, peptone 10g, whole be put into 1000mL water are boiled, and are poured into test tube 1/5 and are formed
Slant medium；
(2) prepared by two level kind：Wheat is boiled into wheat without white point, pulls out and admixes potassium dihydrogen phosphate, gypsum, brown sugar, is bottled
Two level kind is made in 1h to 25 DEG C of access one-level kind of 125 DEG C of sterilizings；
(3) prepared by culture matrix：After miscellaneous tree wood fragments bits are crushed plus water ferments 20-24 h under 20-25 DEG C of environment, will be red
Sugar, lime, corn flour, wheat bran, calcium superphosphate and potash fertilizer are added and stirred, and are fitted into plastic bag, are positioned in sterilizing cabinet often
Pressure sterilizing 12h, it is standby to be cooled to normal temperature and pressure in autoclaving 6h；
(4) access of cultigen：It in bacterium bag and will be sealed made from two level access step (3), be positioned over 20-25 DEG C, phase
To being cultivated 3-4 months in environment that humidity is 60-70%, bacterium bag is covered with to mycelia, isabelline surface is changed into and is covered with Ru Lei；
(5) by bacterium bag be transferred to temperature be 20-28 DEG C, relative humidity 80%, be millesimal Gao Meng with mass concentration
The interior of sour potassium or soda bath sterilization, opens bacterium bag, overburden soil 1-2cm can fruiting after cultivating 20 days in bacterium bag.
Further, in step (2), the wheat, potassium dihydrogen phosphate, the percentage by weight of lime and brown sugar are wheat
88.8%th, potassium dihydrogen phosphate 0.2%, gypsum 10%, brown sugar 1%.
Further, in step (3), the moisture of the culture matrix is 50-70%.
Further, in step (3), the culture medium is prepared by the raw material of following weight percents：Miscellaneous tree is broken
Wood chip 48-54%, brown sugar 1-2%, lime 3-7%, corn flour 15-25%, wheat bran 15-25%, calcium superphosphate 0.8-1.5% and
Potash fertilizer 0.4-0.5%, and each raw material weight and for 100%.
Further, the culture medium is prepared by the raw material of following weight percents：Miscellaneous tree wood fragments bits 52%,
Brown sugar 1.5%, lime 5%, corn flour 20%, wheat bran 20%, calcium superphosphate 1% and potash fertilizer 0.5%.
Further, it is one or more of to include chestnut, pear tree, wax gourd tree for the miscellaneous tree.
The method have the benefit that：The collybia albuminosa cultivated by the inventive method, achievable whole year can produce
Chicken fir, makes the production of collybia albuminosa not influenceed by season, and by the inventive method, the large-scale production of chicken fir can be achieved；This hair
Bright method production has extraordinary bionical effect, and nutritive value is very high, and fresh mushroom can directly be eaten it raw, and has safe and non-toxic
Effect.
Brief description of the drawings
Fig. 1 is the photo for the chicken fir that the inventive method is cultivated.
Embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described,
Obviously, described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.Based in the present invention
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made, all
Belong to the scope of protection of the invention.
Embodiment 1
A kind of cultural method for manually cultivating black chicken fir, comprises the following steps：
(1) prepared by one-level kind：By potassium dihydrogen phosphate 0.2g, dipotassium hydrogen phosphate 0.2g, agar 20g, glucose 20g, formic acid
5g, gypsum 5g, magnesium sulfate 2g, yeast extract 5g, peptone 10g, whole be put into 1000mL water are boiled, and are poured into test tube 1/5 and are formed
Slant medium；
(2) prepared by two level kind：Wheat is boiled into wheat without white point, pulls out and admixes potassium dihydrogen phosphate, gypsum, brown sugar, is bottled
Two level kind is made in 1h to 25 DEG C of access one-level kind of 125 DEG C of sterilizings；Wheat, potassium dihydrogen phosphate, the percentage by weight of lime and brown sugar
For wheat 88.8%, potassium dihydrogen phosphate 0.2%, gypsum 10%, brown sugar 1%.
(3) prepared by culture matrix：After chestnut wood fragments bits are crushed plus water ferments 24h under 20 DEG C of environment, by brown sugar, stone
Ash, corn flour, wheat bran, calcium superphosphate and potash fertilizer are added and stirred, and are fitted into plastic bag, are positioned over normal-pressure sterilization in sterilizing cabinet
12h, it is standby to be cooled to normal temperature and pressure in autoclaving 6h；
Culture medium is prepared by the raw material of following weight percents：Chestnut wood fragments bits 52%, brown sugar 1.5%, lime
5%th, corn flour 20%, wheat bran 20%, calcium superphosphate 1% and potash fertilizer 0.5%, amount of water are controlled the 60% of full culture medium.
(4) access of cultigen：In bacterium bag and will be sealed made from two level access step (3), be positioned over 20 DEG C, it is relative
Humidity is to be cultivated 3 months in 70% environment, covers with bacterium bag to mycelia, changes into isabelline surface and be covered with Ru Lei；
(5) mushroom producing culture：By bacterium bag be transferred to temperature be 20 DEG C, relative humidity 80%, be one thousandth with mass concentration
Potassium permanganate or soda bath sterilization interior, open bacterium bag, in bacterium bag overburden soil 1cm cultivate 20 days after can fruiting
(as shown in Figure 1).
Embodiment 2
A kind of cultural method for manually cultivating black chicken fir, comprises the following steps：
(1) prepared by one-level kind：By potassium dihydrogen phosphate 0.2g, dipotassium hydrogen phosphate 0.2g, agar 20g, glucose 20g, formic acid
5g, gypsum 5g, magnesium sulfate 2g, yeast extract 5g, peptone 10g, whole be put into 1000mL water are boiled, and are poured into test tube 1/5 and are formed
Slant medium；
(2) prepared by two level kind：Wheat is boiled into wheat without white point, pulls out and admixes potassium dihydrogen phosphate, gypsum, brown sugar, is bottled
Two level kind is made in 1h to 25 DEG C of access one-level kind of 125 DEG C of sterilizings；Wherein, the weight hundred of wheat, potassium dihydrogen phosphate, lime and brown sugar
Divide than being wheat 88.8%, potassium dihydrogen phosphate 0.2%, gypsum 10%, brown sugar 1%.
(3) prepared by culture matrix：After pear tree wood fragments bits are crushed plus water ferments 22h under 23 DEG C of environment, by brown sugar, stone
Ash, corn flour, wheat bran, calcium superphosphate and potash fertilizer are added and stirred, and are fitted into plastic bag, are positioned over normal-pressure sterilization in sterilizing cabinet
12h, it is standby to be cooled to normal temperature and pressure in autoclaving 6h；
Culture medium is prepared by the raw material of following weight percents：Pear tree wood fragments bits 48%, brown sugar 2%, lime
3%th, corn flour 25%, wheat bran 20%, calcium superphosphate 1.5% and potash fertilizer 0.5%, moisture 60%.
(4) access of cultigen：In bacterium bag and will be sealed made from two level access step (3), be positioned over 23 DEG C, it is relative
Humidity is to be cultivated 4 months in 65% environment, covers with bacterium bag to mycelia, changes into isabelline surface and be covered with Ru Lei；
(5) mushroom producing culture：By bacterium bag be transferred to temperature be 24 DEG C, relative humidity 80%, be one thousandth with mass concentration
Potassium permanganate or soda bath sterilization interior, open bacterium bag, in bacterium bag overburden soil 1-2cm cultivate 20 days after can go out
Mushroom.
Embodiment 3
A kind of cultural method for manually cultivating black chicken fir, comprises the following steps：
(1) prepared by one-level kind：By potassium dihydrogen phosphate 0.2g, dipotassium hydrogen phosphate 0.2g, agar 20g, glucose 20g, formic acid
5g, gypsum 5g, magnesium sulfate 2g, yeast extract 5g, peptone 10g, whole be put into 1000mL water are boiled, and are poured into test tube 1/5 and are formed
Slant medium；
(2) prepared by two level kind：Wheat is boiled into wheat without white point, pulls out and admixes potassium dihydrogen phosphate, gypsum, brown sugar, is bottled
Two level kind is made in 1h to 25 DEG C of access one-level kind of 125 DEG C of sterilizings；Wherein, the weight hundred of wheat, potassium dihydrogen phosphate, lime and brown sugar
Divide than being wheat 88.8%, potassium dihydrogen phosphate 0.2%, gypsum 10%, brown sugar 1%.
(3) prepared by culture matrix：After water wax gourd tree wood fragments bits are crushed plus water ferments 20h under 25 DEG C of environment, by brown sugar,
Lime, corn flour, wheat bran, calcium superphosphate and potash fertilizer are added and stirred, and are fitted into plastic bag, are positioned over normal pressure in sterilizing cabinet and go out
Bacterium 12h, it is standby to be cooled to normal temperature and pressure in autoclaving 6h；
Culture medium is prepared by the raw material of following weight percents：Miscellaneous tree wood fragments bits 54%, brown sugar 1%, lime
7%th, corn flour 15%, wheat bran 21.8%, calcium superphosphate 0.8% and potash fertilizer 0.4%, culture medium moisture are 70%.
(4) access of cultigen：In bacterium bag and will be sealed made from two level access step (3), be positioned over 25 DEG C, it is relative
Humidity is to be cultivated 3.5 months in 70% environment, covers with bacterium bag to mycelia, changes into isabelline surface and be covered with Ru Lei；
(5) mushroom producing culture：By bacterium bag be transferred to temperature be 28 DEG C, relative humidity 80%, be one thousandth with mass concentration
Potassium permanganate or soda bath sterilization interior, open bacterium bag, in bacterium bag overburden soil 2cm cultivate 20 days after can fruiting.
Embodiment 4
Each 10 of the collybia albuminosa of culture in embodiment 1,2 and 3, the people of edible mushroom association of Yunnan Province expert 5 is asked to its outward appearance
Evaluated with wild chicken fir similitude, each expert scores three embodiment products, and highest is divided into 10 points, minimum point
For 0 point.
| Expert 1 | Expert 2 | Expert 3 | Expert 4 | Expert 5 | Total score | |
| Embodiment 1 | 9.0 | 8.7 | 9.1 | 8.8 | 9.2 | 44.8 |
| Embodiment 2 | 8.7 | 9.1 | 8.6 | 8.9 | 9.1 | 44.4 |
| Embodiment 3 | 8.5 | 8.8 | 8.2 | 9.1 | 8.6 | 43.2 |
As can be known from the above table, the chicken fir cultivated by the inventive method, its outward appearance and wild chicken fir are closely similar, and similar reaches
To 80-95%.
Embodiment 5
The collybia albuminosa of embodiment 1-3 production is chosen, is directly added into a small amount of green pepper after cleaning up, quick-fried 10-15 minutes,
Product asks 5 Yunnan ordinary consumers to carry out mouthfeel evaluation, and evaluation index includes delicious degree, sweet taste, and highest is divided into 10
Point, it is minimum to be divided into 0 point.
| Evaluation 1 | Evaluation 2 | Evaluation 3 | Evaluation 4 | Evaluation 5 | Total score | |
| Embodiment 1 | 8.3 | 8.1 | 8.5 | 9.0 | 7.9 | 41.6 |
| Embodiment 2 | 9.2 | 8.3 | 8.7 | 9.1 | 9.0 | 44.3 |
| Embodiment 3 | 7.7 | 8.2 | 8.2 | 8.8 | 8.6 | 41.4 |
The inventive method cultivates chicken fir, and mouthfeel is fresh and tender, pleasantly sweet, and overall mouth feel and wild chicken fir mouthfeel are closely similar.
Embodiment 6
The collybia albuminosa of embodiment 1-3 productions is chosen, is cleaned up, sample, which delivers to Yunnan business's mass metering inspection technology service, to be had
Limit company is measured to its nutritive index, as a result as shown in the table.
Nutrient component meter (dry chicken fir)
| Project | Every 100 grams (g) | NRV (%) |
| Energy | 537 kilojoules (kJ) | 6% |
| Protein | 18.1 grams (g) | 30% |
| Fat | 1.7 grams (g) | 3% |
| Carbohydrate | 9.8 grams (g) | 3% |
| Sodium | 40 milligrams (mg) | 2% |
| Phosphorus | 430 milligrams (mg) | 61% |
| Iron | 5.5 milligrams (mg) | 37% |
| Manganese | 0.29 milligram (mg) | 10% |
It should be noted last that：Above example is only to illustrative and not limiting technical scheme, although ginseng
The present invention is described in detail according to above-described embodiment, it will be apparent to an ordinarily skilled person in the art that：Still can be to this
Invention is modified or equivalent substitution, and any modification or partial replacement without departing from the spirit and scope of the present invention, its is equal
It should cover among scope of the presently claimed invention.
Claims (6)
1. a kind of cultural method for manually cultivating grey chicken fir, it is characterised in that comprise the following steps：
(1) prepared by one-level kind：By potassium dihydrogen phosphate 0.2g, dipotassium hydrogen phosphate 0.2g, agar 20g, glucose 20g, formic acid 5g, stone
Cream 5g, magnesium sulfate 2g, yeast extract 5g, peptone 10g, whole be put into 1000mL water are boiled, and are poured into test tube 1/5 and are formed inclined-plane training
Support base；
(2) prepared by two level kind：Wheat is boiled into wheat without white point, pulls out and admixes potassium dihydrogen phosphate, gypsum, brown sugar, is bottled 125 DEG C
Two level kind is made in 1h to 25 DEG C of access one-level kind of sterilizing；
(3) prepared by culture matrix：After miscellaneous tree wood fragments bits are crushed plus water ferments 20-24h under 20-25 DEG C of environment, by brown sugar, stone
Ash, corn flour, wheat bran, calcium superphosphate and potash fertilizer are added and stirred, and are fitted into plastic bag, are positioned over normal-pressure sterilization in sterilizing cabinet
12h, it is standby to be cooled to normal temperature and pressure in autoclaving 6h；
(4) access of cultigen：In bacterium bag and will be sealed made from two level access step (3), be positioned over 20-25 DEG C, it is relatively wet
Spend and cultivated in the environment for 60-70% 3-4 months, cover with bacterium bag to mycelia, change into isabelline surface and be covered with Ru Lei；
(5) mushroom producing culture：By bacterium bag be transferred to temperature be 20-28 DEG C, relative humidity 80%, with mass concentration to be millesimal
Potassium permanganate or the interior of soda bath sterilization, open bacterium bag, overburden soil 1-2cm can fruiting after cultivating 20 days in bacterium bag.
2. the cultural method of grey chicken fir is manually cultivated according to claim 1, it is characterised in that described small in step (2)
Wheat, potassium dihydrogen phosphate, the percentage by weight of lime and brown sugar are wheat 88.8%, potassium dihydrogen phosphate 0.2%, gypsum 10%, red
Sugar 1%.
3. the cultural method of grey chicken fir is manually cultivated according to claim 1, it is characterised in that in step (3), the culture
The moisture of matrix is 50-70%.
4. according to any one of claim 1 or 3 cultural method for manually cultivating grey chicken fir, it is characterised in that step (3)
In, the culture medium is prepared by the raw material of following weight percents：Miscellaneous tree wood fragments bits 48-54%, brown sugar 1-2%, stone
Grey 3-7%, corn flour 15-25%, wheat bran 15-25%, calcium superphosphate 0.8-1.5% and potash fertilizer 0.4-0.5%, and each raw material weight
Amount and for 100%.
5. the cultural method of grey chicken fir is manually cultivated according to claim 4, it is characterised in that the culture medium is by following
The raw material of percentage by weight is prepared：Miscellaneous tree wood fragments bits 52%, brown sugar 1.5%, lime 5%, corn flour 20%, wheat bran
20%th, calcium superphosphate 1% and potash fertilizer 0.5%.
6. manually cultivate the cultural method of grey chicken fir according to claim 5, it is characterised in that the miscellaneous tree include chestnut,
Pear tree, wax gourd tree are one or more of.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112913578A (en) * | 2021-04-02 | 2021-06-08 | 河源市农产品流通中心有限公司 | Planting and cultivating method of black-skin termitomyces albuminosus |
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Application publication date: 20180126 |