CN104187586B - Hericium erinaceus many mushrooms powder and preparation method thereof - Google Patents

Hericium erinaceus many mushrooms powder and preparation method thereof Download PDF

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CN104187586B
CN104187586B CN201410346002.6A CN201410346002A CN104187586B CN 104187586 B CN104187586 B CN 104187586B CN 201410346002 A CN201410346002 A CN 201410346002A CN 104187586 B CN104187586 B CN 104187586B
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enzymolysis
powder
temperature
hericium erinaceus
mushroom
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CN104187586A (en
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李建树
邵素英
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Yichun Shanzhen Dawang Food Technology Co., Ltd.
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Yiwu Juhui Biotechnology Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L31/00Edible extracts or preparations of fungi; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The invention discloses a kind of Hericium erinaceus many mushrooms powder and preparation method thereof, belong to edible mushroom field of deep.Described Hericium erinaceus many mushrooms powder, comprises the component of following parts by weight: hickory chick enzymolysis powder 5-15, Hericium erinaceus mushroom enzymolysis powder 30-50, animal protein hydrolysis powder 2-5, salt 0.05-0.2, described Hericium erinaceus many mushrooms powder takes each raw material by formula, mix rear microwave disinfection, packaging is product.Product of the present invention has mushroom fragrance to be given prominence to, and taste is suitable for, nutritious feature, by enzymolysis processing, makes the degraded total amino acid content that in its fructification tissue, protein obtains to a certain extent improve 20-37% than non-enzymolysis processing; And owing to adopting special high temperature centrifugal separation process, make edible fungi polysaccharide content in product improve 16-35% simultaneously; Simultaneously due to the good compatibility of the various nutritional labelings of enzymolysis generation, make product of the present invention not only have good seasoning function but also there is good nutritious tonifying function.

Description

Hericium erinaceus many mushrooms powder and preparation method thereof
Technical field
The invention belongs to edible mushroom field of deep, particularly a kind of edible fungus and preparation method thereof.
Background technology
Hericium erinaceus (formal name used at school: Hericiumerinaceus), cries Hericium erinaceus again, only gains the name because exactly liking hedgehog hydnum.Monkey mushroom, hedgehog hydnum, monkey mushroom, is the luxury dish of Chinese tradition, meat is tender, taste is fragrant, tasty.One of four large famous dishes (hedgehog hydnum, bear's paw, sea cucumber, shark's fin).There is the title of " mountain delicacy hedgehog hydnum, seafood delights shark's fin ".The mushroom of this hedgehog fungus section, cap surface is long young pilose antler shape corn, is about 1 ~ 3 centimetre, its fructification is round and thick, white time fresh, after dry by pale yellow to light brown, the narrow or slightly short handle of base portion, top is expanded, diameter 3.5 ~ 10 centimetres, looks far into the distance like spun gold hedgehog hydnum, therefore claims " Hericium erinaceus ", again as hedgehog, therefore there is again the title of " hedgehog bacterium ".Hericium erinaceus is delicious incomparable mountain delicacy, and bacterial context is fresh and tender, aromatic good to eat, has the title of " in element meat or fish ".
Hickory chick is under the jurisdiction of Ascomycotina (Ascomycotina), discomycete, Pezizale, Morchellaceae, morchella, and hickory chick has many small ruts with its cap surface, gains the name like sheep tripe in outward appearance pole.It is a kind of wild famous and precious edible and medical fungi, includes the earliest in Li Shizhen (1518-1593 A.D.) Compendium of Material Medica.The traditional Chinese medical science is used as medicine with Morchella esculenta (L.) Pers sporophore.Hickory chick is foremost delicious edible mushroom in sac fungus, and hickory chick contains protein 22.1%, fat 3.8%, polysaccharide 16.3%; And the unrighted acid in fat is 5 to 3 with the ratio of saturated fatty acid, the linoleic acid useful to human body accounts for 56% of fatty acid total amount.The amino acid of needed by human is complete, and its content is 5 times of Hericium erinaceus.Element also containing needed by human such as abundant potassium, phosphorus, calcium, iron, magnesium, zinc and selenium.Wherein the content of potassium, phosphorus is 4 times and 2 times of Cordyceps sinensis respectively, and Zn content is 9 times of Cordyceps sinensis.Selenium then can change carcinogenic metabolism direction and anti-oxidant, and thus hickory chick is described as " anticancer star ".
Mushroom, also known as mushroom, dried mushroom, is a kind of edible mushroom human body being had to stronger nutrition health-care functions.According to analysis, containing protein 18.4g, fatty 4.8g, carbohydrate 71g (wherein sugar accounts for 35.1g) in 100g fresh mushroom, and containing calcium 120mg, phosphorus 420mg, iron 25mg, vitaminB10 .07mg, vitamin B2 1.13mg, and vitamin C, more than 30 kind of enzyme and 16 seed amino acids, in addition also containing rare ergosterol in general vegetables (generating the precursor substance of vitamin D in human body) and the composition such as double stranded RNA, lentinan with immunologic function.
At present the research of Mushroom production, mushroom processing is come into one's own day by day, " food and biotechnology journal " volume the 2nd March the 26th in the 2007th phase " Study on Liquid Fermentation Technology of Morchella esculenta " has been done to dissolve in research to hickory chick liquid deep layer fermenting, and the best medium proportioning of selection is: corn flour 4.0g/dL, glucose 1.0g/dL, analysis for soybean powder 2.0g/dL, dusty yeast 0.3g/dL, KH 2pO 40.2g/dL, MgSO 40.1g/dL, CaSO 40.1g/dL; Selection optimal culture conditions is: 24 DEG C, and the shaking flask liquid amount of initial pH5.8,250mL is 100mL, inoculum concentration 10mL, shaking flask rotating speed 140r/min, and fermentation time is 108h.On market, the kind of mushroom converted products mainly concentrates on fresh goods, dried product and canned pack three aspects, publication number is the edible mushroom powder that the Chinese invention " mushroom powder and production technology thereof " of CN1136408A discloses in solid powdery, and production technology is: fresh mushroom, rinsing, fragmentation, breaking-wall cell, separation, ultrafiltration, oven dry.Technique is simple but nutrition and the reservation of delicate flavour composition are not fine.Publication number is that the Chinese invention " a kind of compound mushroom flavoring and preparation method thereof " of CN101617808A discloses the flavoring that a kind of primary raw material is agaricus bisporus powder, straw mushroom powder, sodium glutamate etc., wherein the preparation method of mushroom powder is: after fresh mushroom rinsing, boiling, pulverizing, defibrination, homogeneous, then obtains through concentrated, spraying dry.Also there is raw material extraction and application rate not high, nutrition and delicate flavour retain bad problem.Publication number be the Chinese invention " a kind of edible fungus composition of disease preventing and treating " of CN101455332A disclose one can effective disease preventing and treating, the edible fungus composition taken good care of one's health, be made up of the raw material of following percentage by weight: auricularia auriculajudae 8-10%, straw mushroom 9-11%, mushroom 7-9%, mushroom 9-11%, asparagus 11-13%, white fungus 10-12%, glossy ganoderma 12-14%, flat mushroom 7-9%, hedgehog hydnum 8-10%, spice 2-3%, flavouring 2-3% and water 4-6%, total amount 100%, it is first by auricularia auriculajudae, straw mushroom, mushroom, mushroom, asparagus, white fungus, glossy ganoderma, flat mushroom and hedgehog hydnum cutting slivering or block, then spice is added, flavouring and water, in placement pressure cooker, high temperature 120-150 DEG C, boiling 5-10 minute, let cool, tinning or bottling or pack, .Publication number is the manufacture craft that the Chinese invention " a kind of anti-tumor healthcare mushroom porridge " of CN103494180A provides a kind of anti-tumor healthcare mushroom porridge, this mushroom porridge comprises Hericium erinaceus, northern hickory chick, asparagus, mushroom, white fungus, flat mushroom, pleurotus eryngii, seafood mushroom, crab flavour mushroom, tomato, the seed of Job's tears, rice etc., each raw material is allocated in proportion, selected ingredient requirement green high quality, without disease and pest; Preparation flow comprises screening and pretreatment, boiling, tinning, the sterilizing etc. of raw material, and manufacturing process does not add any anticorrisive agent and food additives; Be characterized as: to have the edible mushroom of antitumor efficacy for primary raw material, and raw material is fresh fruit body of edible fungi, arranging in pairs or groups with antitumor vegetables, cereal uses, and enhances value and the nutritive value of various raw material.This mushroom porridge comprises various active material as hickory chick element, Flammulin, lentinacin etc., and mushroom taste is strong, nutritious, has significantly prevention and health-care effect to develop immunitypty, pre-preventing tumor.This technological operation is simple, suitable factorial praluction.Publication number is the preparation method that the Chinese invention " instant edible mushroom and preparation method thereof " of CN101238878A provides a kind of instant edible mushroom, be characterised in that the flavoring of use comprises mushroom powder, this mushroom powder comprises matsutake dry powder 9-11 by mass fraction, Boletus luridus dry powder 18-22, flower mushroom dry powder 9-11, HEIHUZHANG fungus dry powder 9-11, hickory chick dry powder 9-11, Termitomyces albuminosus dry powder 9-11, Boletus aereus dry powder 90-110, Hericium erinaceus dry powder 9-11, has preparation method's technological process simple, with low cost; The instant product mouthfeel made is excellent, non-greasy, has the feature of the natural fresh perfume (or spice) of strong mushroom.Described mushroom powder is obtained, granularity 40-120 order lower than 12% rear grinding to water content by corresponding fresh feed bacterium natural air drying.
How to make full use of mushroom nutritious, delicious in taste, be rich in the feature of various nutritional labeling, improve raw material extraction, utilization rate, exploitation has the flavoring products of good nutrition health care, meet and enrich the growing material requisite of people, developing edible mushroom new application is edible mushroom field of deep problem demanding prompt solution.
Summary of the invention
The technical problem that the present invention solves is to provide a kind of Hericium erinaceus many mushrooms powder, and provides the preparation method of this Hericium erinaceus many mushrooms powder.
Described Hericium erinaceus many mushrooms powder, comprises the component of following parts by weight: hickory chick enzymolysis powder 5-15, Hericium erinaceus mushroom enzymolysis powder 30-50, animal protein hydrolysis powder 2-5, salt 0.05-0.2;
The preparation process of described hickory chick enzymolysis powder is as follows: hickory chick prepares through fermentation method, adjusts to 40-60 DEG C after fermentation ends, adds the cellulase degradation process of zymotic fluid weight 0.5-2%, temperature 30 ~ 50 DEG C, pH4 ~ 5, time 0.5-1 hour; Add the flavor protease enzymolysis processing of zymotic fluid weight 0.3-1%, hydrolysis temperature 45-50 DEG C, pH value 6-7, enzymolysis time 1-2 hour; Adjustment temperature keeps 5-10 minute to 90-110 DEG C, maintains this temperature centrifugation, keeps rotating speed at more than 4000rpm; Centrifuged supernatant drying obtains hickory chick enzymolysis powder.
Hericium erinaceus mushroom enzymolysis powder preparation process is as follows: mushroom, after hericium erinaceus fruiting body and Radix Isatidis are pulverized, crushed material mixes with the ratio of 1:3-6 with water, adjustment temperature is 40-60 DEG C, pH is 5.0-7.0, adjust temperature after adding mushroom and hericium erinaceus fruiting body quality 0.1-0.3% cellulase degradation 1-1.5 hour and keep 2-5 minute to 80-95 DEG C, adjustment temperature is to 50 DEG C, interpolation mushroom and hericium erinaceus fruiting body quality 0.1-0.3% flavor protease carry out enzymolysis subsequently, hydrolysis temperature 40-50 DEG C, pH value 6-7, enzymolysis time 1-2 hour, control temperature obtains supernatant 90-95 DEG C of centrifugation, supernatant spraying dry obtains Hericium erinaceus mushroom enzymolysis powder.Described mushroom, hericium erinaceus fruiting body and Radix Isatidis are all crushed to particle diameter at below 0.5mm.The parts by weight of mushroom, Hericium erinaceus, Radix Isatidis are respectively mushroom 5-8, Hericium erinaceus 15-20, Radix Isatidis 0.2-0.5;
Animal protein hydrolysis powder, preparation method thereof is as follows: get quantitative animal flesh and rub through meat grinder, animal flesh after rubbing and water mix with the ratio of 1:5.1-6 and through milling treatment of colloid, adjustment colloid mill stator and the gap of rotor are 105-120 micron, colloid mill flow-control be 0.2-0.5 ton/hour, it is 40-60 DEG C that animal flesh after milling treatment of colloid is adjusted temperature, pH is 5.0-7.0, add the papain enzymolysis 60-100 minute of animal flesh quality 0.12-0.25%, 5-10 DEG C is cooled to keep 1.5-2 hour, increase the temperature to 85-95 DEG C and keep 10-25 minute, control temperature is to 40-50 DEG C, add animal flesh quality 0.12-0.25% flavor protease and carry out enzymolysis, hydrolysis temperature 40-50 DEG C, pH value 6-7, enzymolysis time 1-2 hour, obtain animal protein factor digest, control temperature keeps 2-3 minute at 90 DEG C, adjustment temperature is at 50 DEG C of centrifugal acquisition supernatants, centrifugal rotational speed 4000-5000rpm, centrifugation time 1-2 minute, centrifuged supernatant spraying dry obtains animal protein hydrolysis powder.
Described animal flesh is chicken;
The enzyme preparation of adding in the present invention is purchased from Novi's letter, Beijing Dong Huadeng enzyme preparation company.
The root of bidentate achyranthes and the bark of eucommia enzymolysis liquid of 10-20% is added in toadstool ferment culture medium;
The described root of bidentate achyranthes and bark of eucommia enzymolysis liquid preparation method as follows: the interpolation mass ratio of the root of bidentate achyranthes and the bark of eucommia is 1:2, both were pulverized 100 mesh sieves, crushed mixture is added 6 times of water-soluble solutions, the cellulase degradation of interpolation mixture quality 2.5% 35 minutes, adjustment temperature is 40-60 DEG C, pH is 5.0-7.0, keeps 30 ~ 60 minutes, obtains the root of bidentate achyranthes and bark of eucommia enzymolysis liquid.
Product preparation process of the present invention is as follows:
Take each raw material by formula, mix rear microwave disinfection, packaging is product.
Product of the present invention can directly eat or be dissolved in water edible, also can add in other food and use.
Beneficial effect
This product has mushroom fragrance to be given prominence to, and taste is suitable for, nutritious feature, both can make flavoring, can help food separately again, is the good merchantable brand of tourism at home.By enzymolysis processing, make protein in its fructification tissue obtain degraded to a certain extent, utilize amino acid analyzer to measure, total amino acid content improves 20-37% than non-enzymolysis processing; And owing to adopting special high temperature centrifugal separation process, effectively raise the water-soluble effect of product of the present invention, make edible fungi polysaccharide content in product improve 16-35% simultaneously, effectively improve product quality; Owing to the addition of Hericium erinaceus and animal protein factor digest makes product of the present invention delicious in taste, the good compatibility of the various nutritional labelings simultaneously produced due to enzymolysis, makes product of the present invention not only have good seasoning function but also has good nutritious tonifying function.
The preparation of animal protein hydrolysis powder, high-pressure homogeneous, enzymolysis high-temperature process in mid-term and subsection enzymolysis treatment process is adopted to combine, be beneficial to enzyme to play a role, improve enzymolysis efficiency and nutriment stripping, improve nutritive value and the good taste of product, this product good mouthfeel under without the condition of flavouring, taste appraisal score value uses flavouring product higher than related category.
This product special flavour is delicious, and being rich in multiple nutrients functional component, is a kind of edible mushroom deep processed product collecting nutrition, nourishing, adjusting immunity of human body function.This product instant, can be used as common food flavoring or chafing dish bottom flavorings eats, also direct-edible.
Detailed description of the invention
The following examples can make the present invention of those skilled in the art's comprehend, but do not limit the present invention in any way.
Embodiment 1
A kind of Hericium erinaceus many mushrooms powder, parts by weight are composed as follows: hickory chick enzymolysis powder 10, Hericium erinaceus mushroom enzymolysis powder 40, animal protein hydrolysis powder 3, salt 0.1;
The preparation process of described hickory chick enzymolysis powder is as follows: hickory chick prepares through fermentation method, adjusts to 50 DEG C after fermentation ends, adds the cellulase degradation process of zymotic fluid weight 1%, temperature 45 C, pH5,1 hour time; Add the flavor protease enzymolysis processing of zymotic fluid weight 0.5%, hydrolysis temperature 50 DEG C, pH value 7, enzymolysis time 2 hours; Adjustment temperature keeps 7 minutes to 100 DEG C, maintains this temperature centrifugation, keeps rotating speed at 4000rpm; Centrifuged supernatant drying obtains hickory chick enzymolysis powder.
Hericium erinaceus mushroom enzymolysis powder preparation process is as follows: mushroom, after hericium erinaceus fruiting body and Radix Isatidis are pulverized, crushed material mixes with the ratio of 1:5 with water, adjustment temperature is 50 DEG C, pH is 6.0, the cellulase degradation adding mushroom and hericium erinaceus fruiting body quality 0.2% adjusts temperature and keeps 3 minutes to 90 DEG C after 1 hour, adjustment temperature is to 50 DEG C, interpolation mushroom and hericium erinaceus fruiting body quality 0.2% flavor protease carry out enzymolysis subsequently, hydrolysis temperature 45 DEG C, pH value 6, enzymolysis time 1 hour, control temperature obtains supernatant 95 DEG C of centrifugations, supernatant spraying dry obtains Hericium erinaceus mushroom enzymolysis powder.Described mushroom, hericium erinaceus fruiting body and Radix Isatidis are all crushed to particle diameter at below 0.5mm.Mushroom, Hericium erinaceus and Radix Isatidis parts by weight are respectively mushroom 6, Hericium erinaceus 16, Radix Isatidis 0.5;
Animal protein hydrolysis powder, preparation method thereof is as follows: get animal flesh and rub through meat grinder, animal flesh after rubbing and water mix with the ratio of 1:5.5 and through milling treatment of colloid, the gap of adjustment colloid mill stator and rotor is 115 microns, and colloid mill flow-control is 0.3 ton/hour, it is 50 DEG C that animal flesh after milling treatment of colloid is adjusted temperature, pH is 6.0, add the papain enzymolysis 80 minutes of 0.20%, 8 DEG C are cooled to keep 1.5 hours, increase the temperature to 90 DEG C to keep 15 minutes, control temperature is to 45 DEG C, add animal flesh quality 0.2% flavor protease and carry out enzymolysis, hydrolysis temperature 45 DEG C, pH value 7, enzymolysis time 1 hour, obtain animal protein factor digest, control temperature keeps 2-3 minute at 90 DEG C, adjustment temperature is at 50 DEG C of centrifugal acquisition supernatants, centrifugal rotational speed 4500rpm, centrifugation time 2 minutes, centrifuged supernatant spraying dry obtains animal protein hydrolysis powder.
Described animal flesh is chicken;
The preparation method of hickory chick is as follows:
(1) preparation of described fermented morel liquid: adopt slant strains to spread cultivation step by step and obtain hickory chick fungal fermented filtrate;
(1) first order seed is cultivated: hickory chick slant strains accessed in 500 ml shake flasks, fermentation medium loading amount 100 milliliters, rotary shaker 180 revs/min, cultivation temperature 24 DEG C, incubation time 50 hours;
(2) secondary seed cultivate: by first order seed according to 10% inoculum concentration access in 500 milliliters of secondary seed shaking flasks, condition of culture is identical with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed flask with 10% inoculum concentration, fermentation medium loading amount 1000 milliliters, rotary shaker 100 revs/min, cultivation temperature 24 DEG C, incubation time 50 hours;
(4) first class seed pot is cultivated: the first class seed pot being 150L with 10% inoculum concentration access total measurement (volume) by three grades of seeds, fermentation medium loading amount 100L, cultivation temperature 24 DEG C, mixing speed 150 revs/min, ventilation (V/V) 1:0.5, tank pressure 0.05Mpa, incubation time 48 hours;
(5) secondary seed tank is cultivated: it is 1.5 tons of secondary seed tanks that first class seed pot bacterial classification is accessed total measurement (volume) with 10% inoculum concentration, fermentation medium loading amount 1 ton, and condition of culture is identical with first class seed pot;
(6) fermentation tank culture: the fermentation tank by the bacterial classification in secondary seed tank with 10% inoculum concentration access total measurement (volume) being 13 tons, fermentation medium loading amount 10 tons, cultivation temperature 24 DEG C, mixing speed 100 revs/min, ventilation (V/V) 1:0.4, tank pressure 0.05Mpa, incubation time 72 hours.
Described above-mentioned fermented morel liquid fermentation medium consists of: millet 2%, peptone 1%, beef extract 1%, corn, and 2.5%, potassium dihydrogen phosphate 0.5%, the root of bidentate achyranthes and bark of eucommia enzymolysis liquid 15%; Insufficient section water is supplied, pH7.0-7.2,121 DEG C of high pressure steam sterilization 20min.
The described root of bidentate achyranthes and bark of eucommia enzymolysis liquid preparation method as follows: the interpolation mass ratio of the root of bidentate achyranthes and the bark of eucommia is 1:2, both were pulverized 100 mesh sieves, crushed mixture is added 6 times of water-soluble solutions, the cellulase degradation of interpolation mixture quality 2.5% 35 minutes, adjustment temperature is 50 DEG C, pH is 6.0, keeps 45 minutes, obtains the root of bidentate achyranthes and bark of eucommia enzymolysis liquid.
The enzyme preparation of adding in the present invention is purchased from letter enzyme preparation company of Novi.
Product preparation process of the present invention is as follows:
Take each raw material by formula, mix rear microwave disinfection, packaging is product.
This product has mushroom fragrance to be given prominence to, and taste is suitable for, and total amino acid content improves 37% than non-enzymolysis processing; And the water-soluble effect owing to adopting special high temperature centrifugal separation process to effectively raise product of the present invention, make edible fungi polysaccharide content in product improve 30% simultaneously, effectively improve product quality; Make product of the present invention delicious in taste owing to adding animal protein factor digest, simultaneously due to the good compatibility of the various nutritional labelings of enzymolysis generation, make product of the present invention not only have good seasoning function but also have good nutrition nourishing function.
Embodiment 2
A kind of Hericium erinaceus many mushrooms powder, comprises the component of following parts by weight: hickory chick enzymolysis powder 15, Hericium erinaceus mushroom enzymolysis powder 30, animal protein hydrolysis powder 2, salt 0.05;
The preparation process of described hickory chick enzymolysis powder is as follows: hickory chick prepares through fermentation method, adjusts to 40 DEG C after fermentation ends, adds the cellulase degradation process of zymotic fluid weight 2%, temperature 50 C, pH5,0.5 hour time; Add the flavor protease enzymolysis processing of zymotic fluid weight 0.3%, hydrolysis temperature 50 DEG C, pH value 6, enzymolysis time 1 hour; Adjustment temperature keeps 5 minutes to 110 DEG C, maintains this temperature centrifugation, keeps rotating speed at more than 4000rpm; Centrifuged supernatant drying obtains hickory chick enzymolysis powder.
Hericium erinaceus mushroom enzymolysis powder preparation process is as follows: mushroom, after hericium erinaceus fruiting body and Radix Isatidis are pulverized, crushed material mixes with the ratio of 1:6 with water, adjustment temperature is 60 DEG C, pH is 5.0, the cellulase degradation adding mushroom and hericium erinaceus fruiting body quality 0.1% adjusts temperature and keeps 2 minutes to 95 DEG C after 1.5 hours, adjustment temperature is to 50 DEG C, interpolation mushroom and hericium erinaceus fruiting body quality 0.3% flavor protease carry out enzymolysis subsequently, hydrolysis temperature 40 DEG C, pH value 6, enzymolysis time 1 hour, control temperature obtains supernatant 90 DEG C of centrifugations, supernatant spraying dry obtains Hericium erinaceus mushroom enzymolysis powder.Described mushroom, hericium erinaceus fruiting body and Radix Isatidis are all crushed to particle diameter at below 0.5mm.The parts by weight of mushroom, Hericium erinaceus, Radix Isatidis are respectively mushroom 8, Hericium erinaceus 20, Radix Isatidis 0.2;
Animal protein hydrolysis powder, preparation method thereof is as follows: get quantitative animal flesh and rub through meat grinder, animal flesh after rubbing and water mix with the ratio of 1:6 and through milling treatment of colloid, the gap of adjustment colloid mill stator and rotor is 120 microns, and colloid mill flow-control is 0.5 ton/hour, it is 60 DEG C that animal flesh after milling treatment of colloid is adjusted temperature, pH is 5.0, add the papain enzymolysis 60 minutes of animal flesh quality 0.25%, 5 DEG C are cooled to keep 2 hours, increase the temperature to 95 DEG C to keep 10 minutes, control temperature is to 50 DEG C, add animal flesh quality 0.12% flavor protease and carry out enzymolysis, hydrolysis temperature 50 DEG C, pH value 6, enzymolysis time 1 hour, obtain animal protein factor digest, control temperature keeps 2-3 minute at 90 DEG C, adjustment temperature is at 50 DEG C of centrifugal acquisition supernatants, centrifugal rotational speed 5000rpm, centrifugation time 1 minute, centrifuged supernatant spraying dry obtains animal protein hydrolysis powder.
The root of bidentate achyranthes and the bark of eucommia enzymolysis liquid of 10% is added in toadstool ferment culture medium;
The described root of bidentate achyranthes and bark of eucommia enzymolysis liquid preparation method as follows: the interpolation mass ratio of the root of bidentate achyranthes and the bark of eucommia is 1:2, both were pulverized 100 mesh sieves, crushed mixture is added 6 times of water-soluble solutions, the cellulase degradation of interpolation mixture quality 2.5% 35 minutes, adjustment temperature is 40 DEG C, pH is 5.0, keeps 60 minutes, obtains the root of bidentate achyranthes and bark of eucommia enzymolysis liquid.
Described product preparation process of the present invention is with embodiment 1.
This product has mushroom fragrance to be given prominence to, and taste is suitable for, and total amino acid content improves 20% than non-enzymolysis processing; And the water-soluble effect owing to adopting special high temperature centrifugal separation process to effectively raise product of the present invention, make edible fungi polysaccharide content in product improve 35% simultaneously, effectively improve product quality; Make product of the present invention delicious in taste owing to adding animal protein factor digest, simultaneously because enzymolysis Radix Isatidis, the root of bidentate achyranthes and the bark of eucommia make the active ingredient of Chinese medicine effectively be discharged, improve wherein the result of extraction of plant polyose, in product, plant polyose is through enzymolysis and zymotechnique process, and in hickory chick enzymolysis powder, the content raising of plant polyose improves 8-19% than contrast (not having the enzymolysis processing root of bidentate achyranthes and the bark of eucommia); Therefore, the good compatibility of the various nutritional labelings of product of the present invention, makes product of the present invention not only have good seasoning function but also have good nutrition nourishing function.
Embodiment 3
A kind of Hericium erinaceus many mushrooms powder, parts by weight are composed as follows: hickory chick enzymolysis powder 5, Hericium erinaceus mushroom enzymolysis powder 50, animal protein hydrolysis powder 5, salt 0.2;
The preparation process of described hickory chick enzymolysis powder is as follows: hickory chick prepares through fermentation method, adjusts to 60 DEG C after fermentation ends, adds the cellulase degradation process of zymotic fluid weight 0.5%, temperature 30 DEG C, pH4,1 hour time; Add the flavor protease enzymolysis processing of zymotic fluid weight 1%, hydrolysis temperature 45 DEG C, pH value 7, enzymolysis time 2 hours; Adjustment temperature keeps 10 minutes to 90 DEG C, maintains this temperature centrifugation, keeps rotating speed at more than 4000rpm; Centrifuged supernatant drying obtains hickory chick enzymolysis powder.
Hericium erinaceus mushroom enzymolysis powder preparation process is as follows: mushroom, after hericium erinaceus fruiting body and Radix Isatidis are pulverized, crushed material mixes with the ratio of 1:3 with water, adjustment temperature is 40 DEG C, pH is 7.0, the cellulase degradation adding mushroom and hericium erinaceus fruiting body quality 0.3% adjusts temperature and keeps 5 minutes to 80 DEG C after 1 hour, adjustment temperature is to 50 DEG C, interpolation mushroom and hericium erinaceus fruiting body quality 0.1% flavor protease carry out enzymolysis subsequently, hydrolysis temperature 50 DEG C, pH value 7, enzymolysis time 2 hours, control temperature obtains supernatant 95 DEG C of centrifugations, supernatant spraying dry obtains Hericium erinaceus mushroom enzymolysis powder.Described mushroom, hericium erinaceus fruiting body and Radix Isatidis are all crushed to particle diameter at below 0.5mm.The parts by weight of mushroom, Hericium erinaceus, Radix Isatidis are respectively mushroom 5, Hericium erinaceus 15, Radix Isatidis 0.5;
Animal protein hydrolysis powder, preparation method thereof is as follows: get quantitative animal flesh and rub through meat grinder, animal flesh after rubbing and water mix with the ratio of 1:5.1 and through milling treatment of colloid, the gap of adjustment colloid mill stator and rotor is 105 microns, and colloid mill flow-control is 0.2 ton/hour, it is 40 DEG C that animal flesh after milling treatment of colloid is adjusted temperature, pH is 7.0, add the papain enzymolysis 100 minutes of animal flesh quality 0.12%, 10 DEG C are cooled to keep 1.5 hours, increase the temperature to 85 DEG C to keep 25 minutes, control temperature is to 40 DEG C, add animal flesh quality 0.25% flavor protease and carry out enzymolysis, hydrolysis temperature 40 DEG C, pH value 7, enzymolysis time 2 hours, obtain animal protein factor digest, control temperature keeps 2-3 minute at 90 DEG C, adjustment temperature is at 50 DEG C of centrifugal acquisition supernatants, centrifugal rotational speed 4000rpm, centrifugation time 2 minutes, centrifuged supernatant spraying dry obtains animal protein hydrolysis powder.
The root of bidentate achyranthes and the bark of eucommia enzymolysis liquid of 20% is added in toadstool ferment culture medium;
The described root of bidentate achyranthes and bark of eucommia enzymolysis liquid preparation method as follows: the interpolation mass ratio of the root of bidentate achyranthes and the bark of eucommia is 1:2, both were pulverized 100 mesh sieves, crushed mixture is added 6 times of water-soluble solutions, the cellulase degradation of interpolation mixture quality 2.5% 35 minutes, adjustment temperature is 60 DEG C, pH is 7.0, keeps 30 minutes, obtains the root of bidentate achyranthes and bark of eucommia enzymolysis liquid.
Described product preparation process of the present invention is with embodiment 1.
This product embodiments 1 product select in the winter time Beijing area 100 ages 60-75 year weak easy catching a cold the elderly's edible January continuously, edible 100 grams of every day, with edible front and edible rear effectiveness comparison, the results are shown in Table 1, result shows that this product has positive effect to raising the elderly resistance, enhancing body immunity, and product has good edible and nutritious tonifying effect.Simultaneously can significantly improve sleep quality, improvement rate reaches 66%, and (to have quality length of one's sleep for assessment foundation) many edible this product old men continuously reflect that to take effect for a long time better, and the whole year has no interest, and health is better;
The table 1 eating effect table of comparisons
Number Edible first 30 days times of common cold (total) Times of common cold (total) in 30 days after edible February
100 31 7
Sensory evaluation is tested
30 personnel are invited to carry out sense organ marking to product prepared by the embodiment of the present invention 1, each 30 of personnel; Marking comprises outward appearance (20 points), quality (25 points), local flavor (30 points), chews rear residue sense (25 points) four aspects, and marking personnel independently carry out, and are independent of each other, accurate to ensure judging result.Add up judging result, equal score value gets approximation, retains integer, specifically in table 1:
Table 1 sensory evaluation statistics
Note: show significant difference (P < 0.05) with marking different lowercase alphabet in a line, mark different capitalization and represent that difference extremely significantly (P < 0.01), indicate same letter and represent difference not significantly (P > 0.05).
Above result shows, product prepared by the present invention has good sensory manifestation from outward appearance, quality, local flavor and mouthfeel.

Claims (6)

1. Hericium erinaceus many mushrooms powder, comprise following parts by weight component: hickory chick enzymolysis powder 5-15, Hericium erinaceus mushroom enzymolysis powder 30-50, animal protein hydrolysis powder 2-5, salt 0.05-0.2, it is characterized in that, the preparation process of described hickory chick enzymolysis powder is as follows: hickory chick prepares through fermentation method, adjusts to 40-60 DEG C after fermentation ends, add the cellulase degradation process of zymotic fluid weight 0.5-2%, temperature 30 ~ 50 DEG C, pH4 ~ 5, time 0.5-1 hour; Add the flavor protease enzymolysis processing of zymotic fluid weight 0.3-1%, hydrolysis temperature 45-50 DEG C, pH value 6-7, enzymolysis time 1-2 hour; Adjustment temperature keeps 5-10 minute to 90-110 DEG C, maintains this temperature centrifugation, keeps rotating speed at more than 4000rpm; Centrifuged supernatant drying obtains hickory chick enzymolysis powder.
2. Hericium erinaceus many mushrooms powder according to claim 1, it is characterized in that, described Hericium erinaceus mushroom enzymolysis powder preparation process is as follows: mushroom, after hericium erinaceus fruiting body and Radix Isatidis are pulverized, crushed material mixes with the ratio of 1:3-6 with water, adjustment temperature is 40-60 DEG C, pH is 5.0-7.0, adjust temperature after adding mushroom and hericium erinaceus fruiting body quality 0.1-0.3% cellulase degradation 1-1.5 hour and keep 2-5 minute to 80-95 DEG C, adjustment temperature is to 50 DEG C, interpolation mushroom and hericium erinaceus fruiting body quality 0.1-0.3% flavor protease carry out enzymolysis subsequently, hydrolysis temperature 40-50 DEG C, pH value 6-7, enzymolysis time 1-2 hour, control temperature obtains supernatant 90-95 DEG C of centrifugation, supernatant spraying dry obtains Hericium erinaceus mushroom enzymolysis powder,
Described mushroom, hericium erinaceus fruiting body and Radix Isatidis are all crushed to particle diameter at below 0.5mm;
The parts by weight of described mushroom, Hericium erinaceus, Radix Isatidis are respectively mushroom 5-8, Hericium erinaceus 15-20, Radix Isatidis 0.2-0.5.
3. according to the arbitrary described Hericium erinaceus many mushrooms powder of claim 1-2, it is characterized in that, animal protein hydrolysis powder, preparation method thereof is as follows: get quantitative animal flesh and rub through meat grinder, animal flesh after rubbing and water mix with the ratio of 1:5.1-6 and through milling treatment of colloid, adjustment colloid mill stator and the gap of rotor are 105-120 micron, colloid mill flow-control be 0.2-0.5 ton/hour, it is 40-60 DEG C that animal flesh after milling treatment of colloid is adjusted temperature, pH is 5.0-7.0, add the papain enzymolysis 60-100 minute of animal flesh quality 0.12-0.25%, 5-10 DEG C is cooled to keep 1.5-2 hour, increase the temperature to 85-95 DEG C and keep 10-25 minute, control temperature is to 40-50 DEG C, add animal flesh quality 0.12-0.25% flavor protease and carry out enzymolysis, hydrolysis temperature 40-50 DEG C, pH value 6-7, enzymolysis time 1-2 hour, obtain animal protein factor digest, control temperature keeps 2-3 minute at 90 DEG C, adjustment temperature is at 50 DEG C of centrifugal acquisition supernatants, centrifugal rotational speed 4000-5000rpm, centrifugation time 1-2 minute, centrifuged supernatant spraying dry obtains animal protein hydrolysis powder.
4. Hericium erinaceus many mushrooms powder according to claim 3, is characterized in that, add the root of bidentate achyranthes and the bark of eucommia enzymolysis liquid of 10-20% in toadstool ferment culture medium;
The described root of bidentate achyranthes and bark of eucommia enzymolysis liquid preparation method as follows: the interpolation mass ratio of the root of bidentate achyranthes and the bark of eucommia is 1:2, both were pulverized 100 mesh sieves, crushed mixture is added 6 times of water-soluble solutions, the cellulase degradation of interpolation mixture quality 2.5% 35 minutes, adjustment temperature is 40-60 DEG C, pH is 5.0-7.0, keeps 30 ~ 60 minutes, obtains the root of bidentate achyranthes and bark of eucommia enzymolysis liquid.
5. according to the preparation method of the arbitrary described Hericium erinaceus many mushrooms powder of claim 1-4, it is characterized in that, take each component raw material by formula, mix rear microwave disinfection, packaging is product.
6. the preparation method of Hericium erinaceus many mushrooms powder according to claim 5, it is characterized in that, described Hericium erinaceus many mushrooms powder parts by weight are composed as follows: hickory chick enzymolysis powder 10, Hericium erinaceus mushroom enzymolysis powder 40, animal protein hydrolysis powder 3, salt 0.1;
The preparation process of described hickory chick enzymolysis powder is as follows: hickory chick prepares through fermentation method, adjusts to 50 DEG C after fermentation ends, adds the cellulase degradation process of zymotic fluid weight 1%, temperature 45 C, pH5,1 hour time; Add the flavor protease enzymolysis processing of zymotic fluid weight 0.5%, hydrolysis temperature 50 DEG C, pH value 7, enzymolysis time 2 hours; Adjustment temperature keeps 7 minutes to 100 DEG C, maintains this temperature centrifugation, keeps rotating speed at 4000rpm; Centrifuged supernatant drying obtains hickory chick enzymolysis powder;
Hericium erinaceus mushroom enzymolysis powder preparation process is as follows: mushroom, after hericium erinaceus fruiting body and Radix Isatidis are pulverized, crushed material mixes with the ratio of 1:5 with water, adjustment temperature is 50 DEG C, pH is 6.0, the cellulase degradation adding mushroom and hericium erinaceus fruiting body quality 0.2% adjusts temperature and keeps 3 minutes to 90 DEG C after 1 hour, adjustment temperature is to 50 DEG C, interpolation mushroom and hericium erinaceus fruiting body quality 0.2% flavor protease carry out enzymolysis subsequently, hydrolysis temperature 45 DEG C, pH value 6, enzymolysis time 1 hour, control temperature obtains supernatant 95 DEG C of centrifugations, supernatant spraying dry obtains Hericium erinaceus mushroom enzymolysis powder, described mushroom, hericium erinaceus fruiting body and Radix Isatidis are all crushed to particle diameter at below 0.5mm, mushroom, Hericium erinaceus and Radix Isatidis parts by weight are respectively mushroom 6, Hericium erinaceus 16, Radix Isatidis 0.5,
Animal protein hydrolysis powder, preparation method thereof is as follows: get animal flesh and rub through meat grinder, animal flesh after rubbing and water mix with the ratio of 1:5.5 and through milling treatment of colloid, the gap of adjustment colloid mill stator and rotor is 115 microns, and colloid mill flow-control is 0.3 ton/hour, it is 50 DEG C that animal flesh after milling treatment of colloid is adjusted temperature, pH is 6.0, add the papain enzymolysis 80 minutes of 0.20%, 8 DEG C are cooled to keep 1.5 hours, increase the temperature to 90 DEG C to keep 15 minutes, control temperature is to 45 DEG C, add animal flesh quality 0.2% flavor protease and carry out enzymolysis, hydrolysis temperature 45 DEG C, pH value 7, enzymolysis time 1 hour, obtain animal protein factor digest, control temperature keeps 2-3 minute at 90 DEG C, adjustment temperature is at 50 DEG C of centrifugal acquisition supernatants, centrifugal rotational speed 4500rpm, centrifugation time 2 minutes, centrifuged supernatant spraying dry obtains animal protein hydrolysis powder,
Described animal flesh is chicken;
The preparation method of hickory chick is as follows:
(1) preparation of fermented morel liquid: adopt slant strains to spread cultivation step by step and obtain hickory chick fungal fermented filtrate;
(1) first order seed is cultivated: hickory chick slant strains accessed in 500 ml shake flasks, fermentation medium loading amount 100 milliliters, rotary shaker 180 revs/min, cultivation temperature 24 DEG C, incubation time 50 hours;
(2) secondary seed cultivate: by first order seed according to 10% inoculum concentration access in 500 milliliters of secondary seed shaking flasks, condition of culture is identical with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed flask with 10% inoculum concentration, fermentation medium loading amount 1000 milliliters, rotary shaker 100 revs/min, cultivation temperature 24 DEG C, incubation time 50 hours;
(4) first class seed pot is cultivated: the first class seed pot being 150L with 10% inoculum concentration access total measurement (volume) by three grades of seeds, fermentation medium loading amount 100L, cultivation temperature 24 DEG C, mixing speed 150 revs/min, ventilation V/V1:0.5, tank pressure 0.05Mpa, incubation time 48 hours;
(5) secondary seed tank is cultivated: it is 1.5 tons of secondary seed tanks that first class seed pot bacterial classification is accessed total measurement (volume) with 10% inoculum concentration, fermentation medium loading amount 1 ton, and condition of culture is identical with first class seed pot;
(6) fermentation tank culture: the fermentation tank by the bacterial classification in secondary seed tank with 10% inoculum concentration access total measurement (volume) being 13 tons, fermentation medium loading amount 10 tons, cultivation temperature 24 DEG C, mixing speed 100 revs/min, ventilation V/V1:0.4, tank pressure 0.05Mpa, incubation time 72 hours;
Above-mentioned fermented morel liquid fermentation medium consists of: millet 2%, peptone 1%, beef extract 1%, corn 2.5%, potassium dihydrogen phosphate 0.5%, the root of bidentate achyranthes and bark of eucommia enzymolysis liquid 15%; Insufficient section water is supplied, pH7.0-7.2,121 DEG C of high pressure steam sterilization 20min;
The described root of bidentate achyranthes and bark of eucommia enzymolysis liquid preparation method as follows: the interpolation mass ratio of the root of bidentate achyranthes and the bark of eucommia is 1:2, both were pulverized 100 mesh sieves, crushed mixture is added 6 times of water-soluble solutions, the cellulase degradation of interpolation mixture quality 2.5% 35 minutes, adjustment temperature is 50 DEG C, pH is 6.0, keeps 45 minutes, obtains the root of bidentate achyranthes and bark of eucommia enzymolysis liquid.
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