CN109526548A - A kind of black fungus bag stuff cultivation method based on cotton seed hulls - Google Patents
A kind of black fungus bag stuff cultivation method based on cotton seed hulls Download PDFInfo
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- CN109526548A CN109526548A CN201811426664.9A CN201811426664A CN109526548A CN 109526548 A CN109526548 A CN 109526548A CN 201811426664 A CN201811426664 A CN 201811426664A CN 109526548 A CN109526548 A CN 109526548A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
- A01G18/22—Apparatus for the preparation of culture media, e.g. bottling devices
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/50—Inoculation of spawn
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/69—Arrangements for managing the environment, e.g. sprinklers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/70—Harvesting
Abstract
The present invention relates to edible mushroom technical field, specially a kind of black fungus bag stuff cultivation method based on cotton seed hulls.The invention proposes a kind of black fungus bag stuff cultivation method based on cotton seed hulls mainly includes that S1 chooses parent species, S2 prepare decomposed material, S3 extension original seed, S4 preparation production kind, S5 inoculated and cultured, S6 management of producing mushroom, S7 are harvested.Black fungus bag stuff cultivation method of the present invention, the decomposed material of the cultivation matrix of production, nutrition equilibrium is abundant, it is with strong points to the growth of black fungus, the yield and quality of black fungus can be effectively improved, it is able to suppress, reduces the influence of common bacteria and other pest and disease damages in black fungus production, and the influence to black fungus strain is small.The present invention adds the decomposed material of cultivation matrix of production in the culture medium that extension original seed, preparation produce kind, improves black fungus strain to the adaptability of production medium, mycelia growth fastly, reduces the period for producing fruiting, high financial profit.
Description
Technical field
The present invention relates to edible mushroom technical field, especially a kind of black fungus bag stuff cultivation method based on cotton seed hulls.
Background technique
Black fungus is a kind of edible mushroom full of nutrition, and is the traditional health food in China and exporting.It other
There are many name, because being grown on rotten wood, are similar to the ear of people, therefore named agaric；It is again slim and graceful like moth butterfly, also known as the wooden moth；Because it
Taste is delicious just like chicken, therefore also name tree chicken, the wooden machine (it is machine that Gu Nanchu people, which calls chicken): the agaric of polyphyll is inlayed mutually in the tree,
Just like piece piece floating clouds, and have the title of cloud ear.
The nutritional ingredient of black fungus, is analyzed according to modern science, containing 10.6 grams of protein, fat 0.2 in every 100 grams of dry products
Gram, 65 grams of carbohydrate, 7 grams of crude fibre, 375 milligrams of calcium, 201 milligrams of phosphorus, 185 milligrams of iron, additionally contain vitamin
B10.15 milligrams, 0.55 milligram of vitamin B2,2.7 milligrams of niacin.Therefore, black fungus is always deep is liked by numerous people,
Frequently as cook in various, the ingredient of western famous dish, or stew well with jujube, lotus seeds sugaring, as Jiamei's point suitable for four seasons
The heart, not only clear and melodious delicious, sliding tender refreshing larynx, and play the role of increasing appetite and nourishing and fit keeping function.Black fungus has certain adsorption energy
Power；Play the role of washing stomach and intestine and digestion cellulose clearly to human body.Therefore, it is textile worker, mine worker and barber institute again
A kind of indispensable health food.
Currently, the cultural method of black fungus, mostly using sawdust, wheat bran or rice chaff, cotton seed hulls as raw material, and by stirring,
It mixing, sterilizing, inoculation, management of producing mushroom, is influenced by process, cultivation matrix, unbalanced nutrition do not enrich,
Influence limits the growth of black fungus；Meanwhile cultural method and cultivation matrix mismatch, and make in production process, cultivation matrix
Easily corruption, is subject to the infringement of other bacteriums, pest and disease damage, the equal sharp fall of black fungus yield, quality of production, economic benefit
It is not high.
Summary of the invention
The purpose of the present invention is to provide a kind of black fungus bag stuff cultivation method based on cotton seed hulls, to solve above-mentioned back
The problem of being proposed in scape technology.
To achieve the goals above, the invention provides the following technical scheme:
A kind of black fungus bag stuff cultivation method based on cotton seed hulls, includes the following steps:
S1, parent species are chosen: chooses suitable black fungus parent species, it is spare；
S2, it prepares decomposed material: weighing leaf, lemonene cogongrass in proportion, clean, dry, display 10~50 days, too
It is exposed to the sun after 3~5 days under sun, together with weighed corncob respectively, agricultural crop straw, bagasse, is ground into 1~2 centimeter length
The section shape of degree is simultaneously added in blender, adds weighed cotton seed hulls, rice husk, rice bran, vinasse, acid-sludge, sucrose, microorganism hair
Yeast-like fungi liquid, stirs evenly, and is placed at a temperature of 38~45 DEG C, places 7~15 days obtained matrix mixtures；Then matrix is mixed
Material, which is placed in, to be built heap on the ground and covers film preservation, maintains turned within 18~26 hours when material heap central temperature reaches 60~75 DEG C
Heap, material heap central temperature maintain 20~30 hours when reaching 60~75 DEG C again, then turning 1 time, until compost changes colour, hand
It holds until breaking, the decomposed material of matrix is made；Weigh respectively in proportion gypsum, lime, urea, calcium superphosphate, potassium dihydrogen phosphate,
Sodium bicarbonate, edible bacteria additive, and be added in blender, then the decomposed material of matrix and suitable quantity of water are added into blender,
It stirs evenly, is sealed by fermentation 15~20 days at a temperature of being subsequently placed in 22~25 DEG C, obtains the decomposed material of cultivation matrix；
S3, extension original seed: it is appropriate to weigh the decomposed material of cultivation matrix made from S2 step, cultivates after crushing with basic PDA
Base is mixed to prepare original seed extension culture medium, the black fungus parent species that S1 step is chosen is seeded on original seed extension culture medium, wide-mouth
Cultivar is made until mycelia hair is full into wide-mouth bottle in bottle routine culture；
S4, preparation production kind: it is appropriate to weigh the decomposed material of cultivation matrix made from S2 step, trains after crushing with basic PDA
Feeding base is mixed to prepare production kind and prepares culture medium, after being packed, being sterilized, is inoculated with cultivar obtained in previous step, conventional to train
It supports, until mycelia hair in bacterium bag is full, production kind is made；
S5, inoculated and cultured: aseptically, the decomposed material of residue cultivation matrix made from S2 step packing, sterilizing,
Then inoculation S4 step production kind obtained, and by the bacterium bag of inoculation, it is cultivated under the conditions of being placed in 24~28 DEG C, it is complete to mycelia
Until covering with；
S6, management of producing mushroom: will cover with the bacterium bag of mycelia, move into greenhouse, and pile is at bacterium wall culture, and aperture promotees bud, keep
Day temperature is at 20~24 DEG C in greenhouse, and day and night temperature is at 8~10 DEG C；Light irradiation is scattered, intensity of illumination is 400~900lx；
Relative air humidity is 90%~95%, and ventilation, moisturizing in time, keeping the water content of Bag Material is 70%~80%, rejects in time
Corruption, the bacteria stick polluted；
S7, harvesting: after the black fungus mushroom body in bacteria stick is sufficiently spread out, harvesting in time, and sunning can be prepared by doing black wood
Ear finished product.
Preferably, in S2 step, in parts by weight, the decomposed material of cultivation matrix, raw material respectively include: cottonseed
60~80 parts of shell, 20~30 parts of corncob, 15~25 parts of agricultural crop straw, 10~20 parts of rice husk, 5~10 parts of rice bran, leaf 3
~7 parts, 2~6 parts of lemonene cogongrass, 6~12 parts of vinasse, 3~7 parts of bagasse, 2~6 parts of acid-sludge, 1~3 part of sucrose, gypsum 1
~3 parts, 0.5~2 part of lime, 1~3 part of urea, 1~3 part of calcium superphosphate, 0.2~0.6 part of potassium dihydrogen phosphate, sodium bicarbonate
0.4~1 part, 0.3~1 part of microbial fermentation bacterium solution, edible 0.8~2 part of bacteria additive, appropriate amount of water.
Preferably, the leaf, component include: mongolian oak leaf, eucalyptus leaves, robur leaf, white poplar leaf, Pelargonium roseum,
Peach leaves, cacumen biotae, in parts by weight, each component are respectively as follows: 10~50 parts of mongolian oak leaf, 10~50 parts of eucalyptus leaves, robur leaf 10
~50 parts, 10~50 parts of white poplar leaf, 4~10 parts of Pelargonium roseum, 2~4 parts of peach leaves, 1~3 part of cacumen biotae.
Preferably, in the S3 step, by percentage to the quality, the original seed extends culture medium, and raw material includes: cultivation
The decomposed material 30%~60% of matrix, surplus are basic PDA culture medium.
Preferably, in S4 step, by percentage to the quality, the production kind prepares culture medium, and raw material includes: to plant
Train the decomposed material 60%~90% of matrix, surplus is basic PDA culture medium.
Preferably, the edible bacteria additive is that beneficial natural resources mushroom ecology is precious.
Preferably, the microbial fermentation bacterium solution is beneficial natural resources stalk fermentation special bacteria solution.
Preferably, in S5 step, the sterilizing of the decomposed material of cultivation matrix is sterilized, specially using steam sterilization
It is kept for 9~12 hours afterwards thoroughly in 100 DEG C of steameds.
Preferably, in S5 step, the aseptic condition is referred specifically in desinfection chamber, and uses potassium permanganate, formaldehyde
It is stifling, or use quartz burner sterilization processing.
The beneficial effects of the present invention are:
A kind of black fungus bag stuff cultivation method based on cotton seed hulls of the present invention, with cotton seed hulls, corncob, agricultural crop straw,
Rice husk is primary raw material, and addition leaf, lemonene cogongrass, vinasse, bagasse, acid-sludge, sucrose etc., undergo microbial fermentation bacterium
The decomposed fermentation of liquid and the room temperature of edible bacteria additive are sealed by fermentation, the decomposed material of cultivation of auricularia auricula matrix obtained, nutrition
Balanced, abundant, to black fungus growth, it is with strong points, the yield and quality of black fungus can be effectively improved；Tree is utilized simultaneously
The ingredients such as Pelargonium roseum, peach leaves, cacumen biotae and lemonene cogongrass in leaf, by displaying, being exposed to the sun, decomposed processing,
It is able to suppress, reduces the influence of common bacteria and other pest and disease damages in black fungus production, and the influence to black fungus strain
It is small.The present invention adds the decomposed material of cultivation matrix of production in the culture medium that extension original seed, preparation produce kind, improves black wood
For ear strain to the adaptability of production medium, mycelia growth is fast, reduces the period of production fruiting, improves the production of black fungus
Amount and quality, high financial profit.
Specific embodiment
In order to facilitate the understanding of those skilled in the art, the present invention is further illustrated combined with specific embodiments below.
Embodiment 1:
A kind of black fungus bag stuff cultivation method based on cotton seed hulls, includes the following steps:
S1, parent species are chosen: chooses suitable black fungus parent species, it is spare；
S2, it prepares decomposed material: weighing leaf, lemonene cogongrass in proportion, clean, dry, display 10 days, at sun bottom
Under be exposed to the sun after 5 days, together with weighed corncob respectively, agricultural crop straw, bagasse, be ground into the section of 1~2 centimetre length
Shape is simultaneously added in blender, adds weighed cotton seed hulls, rice husk, rice bran, vinasse, acid-sludge, sucrose, microbial fermentation bacterium
Liquid stirs evenly, and is placed at a temperature of 38 DEG C, places 15 days obtained matrix mixtures；Then matrix mixture is placed in and is built on the ground
Heap simultaneously covers film preservation, and material heap central temperature maintains progress turning in 26 hours when reaching 60 DEG C, and material heap central temperature reaches again
Maintained 30 hours when to 60 DEG C, then turning 1 time, until compost changes colour, hold it is i.e. disconnected until, the decomposed material of matrix is made；It presses
Ratio weighs gypsum, lime, urea, calcium superphosphate, potassium dihydrogen phosphate, sodium bicarbonate, edible bacteria additive respectively, and is added
In blender, then the decomposed material of matrix and suitable quantity of water are added into blender, stir evenly, it is close at a temperature of being subsequently placed in 22 DEG C
Seal ferment 20 days, obtain the decomposed material of cultivation matrix；Wherein, in parts by weight, the decomposed material of above-mentioned cultivation matrix, raw material point
It does not include: 60 parts of cotton seed hulls, 30 parts of corncob, 25 parts of agricultural crop straw, 10 parts of rice husk, 10 parts of rice bran, 3 parts of leaf, lemonene
6 parts of cogongrass, 6 parts of vinasse, 7 parts of bagasse, 6 parts of acid-sludge, 3 parts of sucrose, 3 parts of gypsum, 0.5 part of lime, 3 parts of urea, peroxophosphoric acid
1 part of calcium, 0.6 part of potassium dihydrogen phosphate, 1 part of sodium bicarbonate, 1 part of microbial fermentation bacterium solution, edible 0.8 part of bacteria additive, water are suitable
Amount；Wherein, the leaf, component include: mongolian oak leaf, eucalyptus leaves, robur leaf, white poplar leaf, Pelargonium roseum, peach leaves,
Cacumen biotae, in parts by weight, each component are respectively as follows: 10 parts of mongolian oak leaf, 50 parts of eucalyptus leaves, 10 parts of robur leaf, white poplar leaf 50
Part, 10 parts of Pelargonium roseum, 4 parts of peach leaves, 1 part of cacumen biotae；Edible bacteria additive is that beneficial natural resources mushroom ecology is precious, microorganism
Zymocyte liquid is beneficial natural resources stalk fermentation special bacteria solution.
S3, extension original seed: it is appropriate to weigh the decomposed material of cultivation matrix made from S2 step, cultivates after crushing with basic PDA
Base is mixed to prepare original seed extension culture medium, the black fungus parent species that S1 step is chosen is seeded on original seed extension culture medium, wide-mouth
Cultivar is made until mycelia hair is full into wide-mouth bottle in bottle routine culture；By percentage to the quality, above-mentioned original seed extension culture
Base, it is basic PDA culture medium that raw material, which includes: the decomposed material 30% of cultivation matrix, surplus,.
S4, preparation production kind: it is appropriate to weigh the decomposed material of cultivation matrix made from S2 step, trains after crushing with basic PDA
Feeding base is mixed to prepare production kind and prepares culture medium, after being packed, being sterilized, is inoculated with cultivar obtained in previous step, conventional to train
It supports, until mycelia hair in bacterium bag is full, production kind is made；By percentage to the quality, above-mentioned production kind prepares culture medium,
It is basic PDA culture medium that raw material, which includes: the decomposed material 60% of cultivation matrix, surplus,.
S5, inoculated and cultured: aseptically, the decomposed material of residue cultivation matrix made from S2 step packing, sterilizing,
Then inoculation S4 step production kind obtained, and by the bacterium bag of inoculation, it cultivates under the conditions of being placed in 24 DEG C, is covered with completely to mycelia
Until；Wherein, above-mentioned aseptic condition, refers specifically in desinfection chamber, and uses potassium permanganate, formaldehyde fumigation, or using purple
Outside line fluorescent tube sterilization processing；The sterilizing of the decomposed material of above-mentioned cultivation matrix, is sterilized using steam sterilization, specially at 100 DEG C
Steamed is kept for 9 hours afterwards thoroughly.
S6, management of producing mushroom: will cover with the bacterium bag of mycelia, move into greenhouse, and pile is at bacterium wall culture, and aperture promotees bud, keep
Day temperature is at 20 DEG C in greenhouse, and day and night temperature is at 8 DEG C；Scatter light irradiation, intensity of illumination 400lx；Relative air humidity exists
95%, ventilation, moisturizing in time, keeping the water content of Bag Material is 70%, the bacteria stick rejecting corruption in time, being polluted；
S7, harvesting: after the black fungus mushroom body in bacteria stick is sufficiently spread out, harvesting in time, and sunning can be prepared by doing black wood
Ear finished product.
Embodiment 2:
A kind of black fungus bag stuff cultivation method based on cotton seed hulls, includes the following steps:
S1, parent species are chosen: chooses suitable black fungus parent species, it is spare；
S2, it prepares decomposed material: weighing leaf, lemonene cogongrass in proportion, clean, dry, display 50 days, at sun bottom
Under be exposed to the sun after 3 days, together with weighed corncob respectively, agricultural crop straw, bagasse, be ground into the section of 1~2 centimetre length
Shape is simultaneously added in blender, adds weighed cotton seed hulls, rice husk, rice bran, vinasse, acid-sludge, sucrose, microbial fermentation bacterium
Liquid stirs evenly, and is placed at a temperature of 45 DEG C, places 7 days obtained matrix mixtures；Then matrix mixture is placed in and is built on the ground
Heap simultaneously covers film preservation, and material heap central temperature maintains progress turning in 18 hours when reaching 75 DEG C, and material heap central temperature reaches again
Maintained 20 hours when to 75 DEG C, then turning 1 time, until compost changes colour, hold it is i.e. disconnected until, the decomposed material of matrix is made；It presses
Ratio weighs gypsum, lime, urea, calcium superphosphate, potassium dihydrogen phosphate, sodium bicarbonate, edible bacteria additive respectively, and is added
In blender, then the decomposed material of matrix and suitable quantity of water are added into blender, stir evenly, it is close at a temperature of being subsequently placed in 25 DEG C
Seal ferment 15 days, obtain the decomposed material of cultivation matrix；Wherein, in parts by weight, the decomposed material of above-mentioned cultivation matrix, raw material point
It does not include: 80 parts of cotton seed hulls, 20 parts of corncob, 15 parts of agricultural crop straw, 20 parts of rice husk, 5 parts of rice bran, 7 parts of leaf, lemonene
2 parts of cogongrass, 12 parts of vinasse, 3 parts of bagasse, 2 parts of acid-sludge, 1 part of sucrose, 1 part of gypsum, 2 parts of lime, 1 part of urea, calcium superphosphate
3 parts, 0.2 part of potassium dihydrogen phosphate, 0.4 part of sodium bicarbonate, 0.3 part of microbial fermentation bacterium solution, edible 2 parts of bacteria additive, water it is suitable
Amount；Wherein, the leaf, component include: mongolian oak leaf, eucalyptus leaves, robur leaf, white poplar leaf, Pelargonium roseum, peach leaves,
Cacumen biotae, in parts by weight, each component are respectively as follows: 50 parts of mongolian oak leaf, 10 parts of eucalyptus leaves, 50 parts of robur leaf, white poplar leaf 40
Part, 4 parts of Pelargonium roseum, 2 parts of peach leaves, 3 parts of cacumen biotae；Edible bacteria additive is that beneficial natural resources mushroom ecology is precious, microorganism hair
Yeast-like fungi liquid is beneficial natural resources stalk fermentation special bacteria solution.
S3, extension original seed: it is appropriate to weigh the decomposed material of cultivation matrix made from S2 step, cultivates after crushing with basic PDA
Base is mixed to prepare original seed extension culture medium, the black fungus parent species that S1 step is chosen is seeded on original seed extension culture medium, wide-mouth
Cultivar is made until mycelia hair is full into wide-mouth bottle in bottle routine culture；By percentage to the quality, above-mentioned original seed extension culture
Base, it is basic PDA culture medium that raw material, which includes: the decomposed material 60% of cultivation matrix, surplus,.
S4, preparation production kind: it is appropriate to weigh the decomposed material of cultivation matrix made from S2 step, trains after crushing with basic PDA
Feeding base is mixed to prepare production kind and prepares culture medium, after being packed, being sterilized, is inoculated with cultivar obtained in previous step, conventional to train
It supports, until mycelia hair in bacterium bag is full, production kind is made；By percentage to the quality, above-mentioned production kind prepares culture medium,
It is basic PDA culture medium that raw material, which includes: the decomposed material 90% of cultivation matrix, surplus,.
S5, inoculated and cultured: aseptically, the decomposed material of residue cultivation matrix made from S2 step packing, sterilizing,
Then inoculation S4 step production kind obtained, and by the bacterium bag of inoculation, it cultivates under the conditions of being placed in 28 DEG C, is covered with completely to mycelia
Until；Wherein, above-mentioned aseptic condition, refers specifically in desinfection chamber, and uses potassium permanganate, formaldehyde fumigation, or using purple
Outside line fluorescent tube sterilization processing；The sterilizing of the decomposed material of above-mentioned cultivation matrix, is sterilized using steam sterilization, specially at 100 DEG C
Steamed is kept for 12 hours afterwards thoroughly.
S6, management of producing mushroom: will cover with the bacterium bag of mycelia, move into greenhouse, and pile is at bacterium wall culture, and aperture promotees bud, keep
Day temperature is at 24 DEG C in greenhouse, and day and night temperature is at 10 DEG C；Scatter light irradiation, intensity of illumination 400lx；Relative air humidity
90%, ventilation, moisturizing in time, keeping the water content of Bag Material is 80%, the bacteria stick rejecting corruption in time, being polluted；
S7, harvesting: after the black fungus mushroom body in bacteria stick is sufficiently spread out, harvesting in time, and sunning can be prepared by doing black wood
Ear finished product.
Embodiment 3:
A kind of black fungus bag stuff cultivation method based on cotton seed hulls, includes the following steps:
S1, parent species are chosen: chooses suitable black fungus parent species, it is spare；
S2, it prepares decomposed material: weighing leaf, lemonene cogongrass in proportion, clean, dry, display 30 days, at sun bottom
Under be exposed to the sun after 4 days, together with weighed corncob respectively, agricultural crop straw, bagasse, be ground into the section of 1~2 centimetre length
Shape is simultaneously added in blender, adds weighed cotton seed hulls, rice husk, rice bran, vinasse, acid-sludge, sucrose, microbial fermentation bacterium
Liquid stirs evenly, and is placed at a temperature of 40 DEG C, places 10 days obtained matrix mixtures；Then matrix mixture is placed in and is built on the ground
Heap simultaneously covers film preservation, and material heap central temperature maintains progress turning in 22 hours when reaching 70 DEG C, and material heap central temperature reaches again
Maintained 26 hours when to 65 DEG C, then turning 1 time, until compost changes colour, hold it is i.e. disconnected until, the decomposed material of matrix is made；It presses
Ratio weighs gypsum, lime, urea, calcium superphosphate, potassium dihydrogen phosphate, sodium bicarbonate, edible bacteria additive respectively, and is added
In blender, then the decomposed material of matrix and suitable quantity of water are added into blender, stir evenly, it is close at a temperature of being subsequently placed in 24 DEG C
Seal ferment 16 days, obtain the decomposed material of cultivation matrix；In parts by weight, the decomposed material of above-mentioned cultivation matrix, raw material wrap respectively
It includes: 70 parts of cotton seed hulls, 25 parts of corncob, 20 parts of agricultural crop straw, 15 parts of rice husk, 7.5 parts of rice bran, 5 parts of leaf, lemon grass
Careless 4 parts, 9 parts of vinasse, 5 parts of bagasse, 4 parts of acid-sludge, 2 parts of sucrose, 2 parts of gypsum, 1.2 parts of lime, 2 parts of urea, calcium superphosphate 2
Part, 0.4 part of potassium dihydrogen phosphate, 0.7 part of sodium bicarbonate, 0.7 part of microbial fermentation bacterium solution, edible 1.4 parts of bacteria additive, water are suitable
Amount；Wherein, the leaf, component include: mongolian oak leaf, eucalyptus leaves, robur leaf, white poplar leaf, Pelargonium roseum, peach leaves,
Cacumen biotae；In parts by weight, the leaf, each component are respectively as follows: 40 parts of mongolian oak leaf, 40 parts of eucalyptus leaves, 40 parts of robur leaf, white
10 parts of Poplar leaves, 7 parts of Pelargonium roseum, 3 parts of peach leaves, 2 parts of cacumen biotae；Edible bacteria additive is that beneficial natural resources mushroom ecology is precious,
Microbial fermentation bacterium solution is beneficial natural resources stalk fermentation special bacteria solution.
S3, extension original seed: it is appropriate to weigh the decomposed material of cultivation matrix made from S2 step, cultivates after crushing with basic PDA
Base is mixed to prepare original seed extension culture medium, the black fungus parent species that S1 step is chosen is seeded on original seed extension culture medium, wide-mouth
Cultivar is made until mycelia hair is full into wide-mouth bottle in bottle routine culture；By percentage to the quality, above-mentioned original seed extension culture
Base, it is basic PDA culture medium that raw material, which includes: the decomposed material 45% of cultivation matrix, surplus,.
S4, preparation production kind: it is appropriate to weigh the decomposed material of cultivation matrix made from S2 step, trains after crushing with basic PDA
Feeding base is mixed to prepare production kind and prepares culture medium, after being packed, being sterilized, is inoculated with cultivar obtained in previous step, conventional to train
It supports, until mycelia hair in bacterium bag is full, production kind is made；By percentage to the quality, above-mentioned production kind prepares culture medium,
It is basic PDA culture medium that raw material, which includes: the decomposed material 75% of cultivation matrix, surplus,.
S5, inoculated and cultured: aseptically, the decomposed material of residue cultivation matrix made from S2 step packing, sterilizing,
Then inoculation S4 step production kind obtained, and by the bacterium bag of inoculation, it cultivates under the conditions of being placed in 26 DEG C, is covered with completely to mycelia
Until；Wherein, above-mentioned aseptic condition, refers specifically in desinfection chamber, and uses potassium permanganate, formaldehyde fumigation, or using purple
Outside line fluorescent tube sterilization processing；The sterilizing of the decomposed material of above-mentioned cultivation matrix, is sterilized using steam sterilization, specially at 100 DEG C
Steamed is kept for 11 hours afterwards thoroughly.
S6, management of producing mushroom: will cover with the bacterium bag of mycelia, move into greenhouse, and pile is at bacterium wall culture, and aperture promotees bud, keep
Day temperature is at 22 DEG C in greenhouse, and day and night temperature is at 9 DEG C；Scatter light irradiation, intensity of illumination 700lx；Relative air humidity exists
92%, ventilation, moisturizing in time, keeping the water content of Bag Material is 75%, the bacteria stick rejecting corruption in time, being polluted；
S7, harvesting: after the black fungus mushroom body in bacteria stick is sufficiently spread out, harvesting in time, and sunning can be prepared by doing black wood
Ear finished product.
It is above-mentioned that invention is exemplarily described, it is clear that present invention specific implementation is not subject to the restrictions described above, only
To use this insubstantial improvement that the inventive concept and technical scheme of the present invention carry out, or the not improved structure by invention
Think and technical solution directly applies to other occasions, it is within the scope of the present invention.
Claims (9)
1. a kind of black fungus bag stuff cultivation method based on cotton seed hulls, which comprises the steps of:
S1, parent species are chosen: chooses suitable black fungus parent species, it is spare；
S2, it prepares decomposed material: weighing leaf, lemonene cogongrass in proportion, clean, dry, display 10~50 days, under the sun
It is exposed to the sun after 3~5 days, together with weighed corncob respectively, agricultural crop straw, bagasse, is ground into the section of 1~2 centimetre length
Shape is simultaneously added in blender, adds weighed cotton seed hulls, rice husk, rice bran, vinasse, acid-sludge, sucrose, microbial fermentation bacterium solution,
It stirs evenly, is placed at a temperature of 38~45 DEG C, place 7~15 days obtained matrix mixtures；Then matrix mixture is placed in ground
On build heap and cover film preservation, progress turning in 18~26 hours is maintained when material heap central temperature reaches 60~75 DEG C, in material heap
Heart temperature maintains 20~30 hours when reaching 60~75 DEG C again, then turning 1 time, until compost changes colour, holds until being disconnected,
The decomposed material of matrix is made；It weighs gypsum respectively in proportion, lime, urea, calcium superphosphate, potassium dihydrogen phosphate, sodium bicarbonate, eat
Bacteria additive, and be added in blender, then the decomposed material of matrix and suitable quantity of water are added into blender, it stirs evenly, then sets
It is sealed by fermentation 15~20 days at a temperature of 22~25 DEG C, obtains the decomposed material of cultivation matrix；
S3, extension original seed: it is appropriate to weigh the decomposed material of cultivation matrix made from S2 step, mixed with basic PDA culture medium after crushing
It closes and original seed extension culture medium is made, the black fungus parent species that S1 step is chosen are seeded on original seed extension culture medium, wide-mouth bottle is normal
Cultivar is made until mycelia hair is full into wide-mouth bottle in rule culture；
S4, preparation production kind: it is appropriate to weigh the decomposed material of cultivation matrix made from S2 step, after crushing with basic PDA culture medium
It is mixed to prepare production kind and prepares culture medium, after being packed, being sterilized, cultivar obtained in inoculation previous step, routine culture, directly
Until mycelia hair is full into bacterium bag, production kind is made；
S5, inoculated and cultured: aseptically, the decomposed material of residue cultivation matrix made from S2 step packs, sterilizing, then
Be inoculated with the production kind obtained of S4 step, and by the bacterium bag of inoculation, cultivated under the conditions of being placed in 24~28 DEG C, covered with completely to mycelia for
Only；
S6, management of producing mushroom: will cover with the bacterium bag of mycelia, move into greenhouse, and pile is at bacterium wall culture, and aperture promotees bud, keep greenhouse
Interior day temperature is at 20~24 DEG C, and day and night temperature is at 8~10 DEG C；Light irradiation is scattered, intensity of illumination is 400~900lx；Air phase
To humidity 90%~95%, ventilation, moisturizing in time, keeping the water content of Bag Material is 70%~80%, rejects corruption in time, meets with
The bacteria stick of pollution；
S7, harvesting: after the black fungus mushroom body in bacteria stick is sufficiently spread out, in time harvest, sunning, can be prepared by dry black fungus at
Product.
2. a kind of black fungus bag stuff cultivation method based on cotton seed hulls according to claim 1, which is characterized in that in S2 step
In, in parts by weight, the decomposed material of cultivation matrix, raw material respectively include: 60~80 parts of cotton seed hulls, corncob 20~30
Part, 15~25 parts of agricultural crop straw, 10~20 parts of rice husk, 5~10 parts of rice bran, 3~7 parts of leaf, 2~6 parts of lemonene cogongrass, wine
Poor 6~12 parts, 3~7 parts of bagasse, 2~6 parts of acid-sludge, 1~3 part of sucrose, 1~3 part of gypsum, 0.5~2 part of lime, urea 1~3
Part, 1~3 part of calcium superphosphate, 0.2~0.6 part of potassium dihydrogen phosphate, 0.4~1 part of sodium bicarbonate, microbial fermentation bacterium solution 0.3~1
Part, edible 0.8~2 part of bacteria additive, appropriate amount of water.
3. a kind of black fungus Bag Material cultivation matrix according to claim 1 or 2, which is characterized in that the leaf, component
It include: mongolian oak leaf, eucalyptus leaves, robur leaf, white poplar leaf, Pelargonium roseum, peach leaves, cacumen biotae, in parts by weight, each component
It is respectively as follows: 10~50 parts of mongolian oak leaf, 10~50 parts of eucalyptus leaves, 10~50 parts of robur leaf, 10~50 parts of white poplar leaf, Pelargonium
4~10 parts of certain herbaceous plants with big flowers, 2~4 parts of peach leaves, 1~3 part of cacumen biotae.
4. a kind of black fungus bag stuff cultivation method based on cotton seed hulls according to claim 1, which is characterized in that in S3 step
In, by percentage to the quality, the original seed extends culture medium, and raw material includes: the decomposed material 30%~60% of cultivation matrix, surplus
For basic PDA culture medium.
5. a kind of black fungus bag stuff cultivation method based on cotton seed hulls according to claim 1, which is characterized in that in S4 step
In, by percentage to the quality, the production kind prepares culture medium, and raw material includes: the decomposed material 60%~90% of cultivation matrix, remaining
Amount is basic PDA culture medium.
6. a kind of black fungus Bag Material cultivation matrix according to claim 1, which is characterized in that the edible bacteria additive is
Beneficial natural resources mushroom ecology is precious.
7. a kind of black fungus Bag Material cultivation matrix according to claim 1, which is characterized in that the microbial fermentation bacterium solution
For beneficial natural resources stalk fermentation special bacteria solution.
8. a kind of black fungus bag stuff cultivation method based on cotton seed hulls according to claim 1, which is characterized in that in S5 step
In, the sterilizing of the decomposed material of cultivation matrix is sterilized using steam sterilization, specially 100 DEG C of steameds thoroughly afterwards keep 9~
12 hours.
9. a kind of black fungus bag stuff cultivation method based on cotton seed hulls according to claim 1, which is characterized in that in S5 step
In, the aseptic condition refers specifically in desinfection chamber, and uses potassium permanganate, formaldehyde fumigation, or is sterilized using quartz burner
Processing.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110115199A (en) * | 2019-06-05 | 2019-08-13 | 广西壮族自治区农业科学院微生物研究所 | A method of production cloud ear strain is considered to be worth doing with Eucalyptus |
| CN110583366A (en) * | 2019-09-30 | 2019-12-20 | 山东瑞蕈天库菌种开发有限公司 | Synthetic particle stock culture medium and preparation method and application thereof |
| CN111296178A (en) * | 2020-03-26 | 2020-06-19 | 贵州福瑞斯新能源科技有限公司 | Method for producing oyster mushrooms by coix seed straws |
-
2018
- 2018-11-27 CN CN201811426664.9A patent/CN109526548A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110115199A (en) * | 2019-06-05 | 2019-08-13 | 广西壮族自治区农业科学院微生物研究所 | A method of production cloud ear strain is considered to be worth doing with Eucalyptus |
| CN110583366A (en) * | 2019-09-30 | 2019-12-20 | 山东瑞蕈天库菌种开发有限公司 | Synthetic particle stock culture medium and preparation method and application thereof |
| CN111296178A (en) * | 2020-03-26 | 2020-06-19 | 贵州福瑞斯新能源科技有限公司 | Method for producing oyster mushrooms by coix seed straws |
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