CN105493897A - Industrialized cultivation method of beech mushrooms - Google Patents
Industrialized cultivation method of beech mushrooms Download PDFInfo
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- CN105493897A CN105493897A CN201610010557.2A CN201610010557A CN105493897A CN 105493897 A CN105493897 A CN 105493897A CN 201610010557 A CN201610010557 A CN 201610010557A CN 105493897 A CN105493897 A CN 105493897A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
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Abstract
The invention relates to an industrialized cultivation method of beech mushrooms. A cultivation flow comprises the following steps: checking and accepting raw materials; formulating and blending the materials; filling the materials into bottles; sterilizing; cooling and inoculating; culturing; manually removing old strain blocks and mycoderma; fruiting; and harvesting and packaging. The industrialized cultivation method is characterized in that beech mushroom or hypsizigus marmoreus stem powder or active beech mushroom or hypsizigus marmoreus stem extract powder is added to a cultivated specie culture medium formula; water used in a blending process is a water solution of the active hypsizigus marmoreus stem extract powder; and 15mL-20mL of water supplemented in a process of manually removing the old strain blocks and mycoderma is the water solution of the active hypsizigus marmoreus stem extract powder. The industrialized cultivation method of the beech mushrooms, provided by the invention, has the beneficial effects that the utilization rate and additive value of beech mushroom stem resources are increased, and the quality and yield of industrialized cultivation of the beech mushrooms are improved.
Description
Technical field
The present invention relates to edible mushroom true Ji mushroom technical field of cultivation, especially a kind of hypsizygus marmoreus industrial planting method.
Background technology
Hypsizygus marmoreus (Hypsizygusmarmoreus) has another name called the beautiful gill fungus of spot, the beautiful mushroom of glue, beautiful gill fungus, letter happiness mushroom, glossy ganoderma mushroom etc., it is a brown kind of true Ji mushroom (Hypsizygusmarmoreus), its Systematic Position is the same with true Ji mushroom, be subordinate to mycota (Fungi), Basidiomycotina ((Basidiomycotina), Hymenomycetes (Hymenomycetes), Agaricales (Agaricales), Bai Mo section (Tricholomataceae), beautiful gill fungus belongs to (Hypsizigus), that a kind of edible the holding concurrently in north temperate zone medicinal treasures famous and precious edible mushroom, have " fragrant at matsutake in Japan, taste is beautiful gill fungus " say, due to mouthfeel and the crab fragrance of hypsizygus marmoreus uniqueness, make the favor of its extremely consumer.In recent years, hypsizygus marmoreus factory culture rises gradually, the scale of factory culture expands year by year, but because hypsizygus marmoreus cultivation period is long, cultivation difficulty is very large, the risk of investment is comparatively large, and the actual production of hypsizygus marmoreus factory culture and quality are not high, and Business Economic Benefit is low, because hypsizygus marmoreus pin handle is due to characteristics such as its fibrosis are high and palatability is poor, cause the wasting of resources processing or be often dropped in commercial treatment.
Summary of the invention
In order to overcome the deficiency that current hypsizygus marmoreus factory culture exists, the invention provides and a kind ofly can significantly improve the availability of hypsizygus marmoreus factory culture seed output and quality and hypsizygus marmoreus pin handle resource and the hypsizygus marmoreus industrial planting method of added value.
The technical solution adopted for the present invention to solve the technical problems is: a kind of hypsizygus marmoreus industrial planting method, the flow process of cultivation: raw-material examination---formula, spice---bottling---sterilizing---cooling,------gather, packaging in cultivation in inoculation by mycelium stimulation---fruiting---.
Raw-material examination:
Wood chip: (pH value: 7.2-7.7, water content: 60%-70%, granularity: below 2mm, silt content: below 3-5%, organoleptic indicator: leaf wood seeds, without going mouldy);
Cotton seed hulls: (pH value: 6.2-7.3, water content: less than 13%, below 3mm, less than 1%, cotton-wool is few, without going mouldy, insect);
Corncob: (pH value: 6.0-7.4, water content: less than 14%, below 3mm, less than 1%, nothing is gone mouldy, impurity, and dust is few);
Rice bran: (pH value: 6.2-7.4, water content: less than 14%, 4 mesh sieve percent of pass 100%, less than 1%, fresh, without going mouldy, becoming sour);
Corn flour: (pH value: 6.3-7.4, water content: less than 14%, 4 mesh sieve percent of pass 100%, less than 1%, fresh, without going mouldy, insect, to become sour);
Wheat bran: (pH value: 6.2-6.8, water content: less than 14%, below 2mm, less than 1%, dry, without going mouldy, becoming sour);
Calcium carbonate: (pH value: more than 11, water content: 4 mesh sieve percent of pass 100%, 0 are Powdered, without making moist, luming).
Formula:
Cultivar culture medium formula is by weight percentage: deciduous species wood chip 22-37%, cotton seed hulls 9-11%, corncob 17-19%, rice bran 22-24%, corn flour 3-5%, wheat bran 11-13%, calcium carbonate 0.8-1%, other raw material 0.2-5%.
Spice:
Under normal temperature, normal pressure, above-mentioned Cultivar culture medium formulation material is sent into automatic stirring machine, carry out spice.
Bottling:
Under normal temperature, normal pressure, above-mentioned spice is sent into automatic bottling machine charging, adopt 1100mL plastic bottle (70mm bore) to carry out bottle cultivation, every bottled wet feed 700g, water content 63%-65%, pH value is between 7.0-7.8.
Sterilizing:
Above-mentioned bottle is planted material and sends into sterilizer sterilizing, temperature 121 DEG C, high pressure 2Mpa, lower sterilizing 3h-4h.
Cooling:
The planting material after bacterium of having gone out is positioned over cooling chamber cooling, is cooled to less than 22 degree, composts or fertilisers of cultivating could be pushed into transfer room inoculation.
Inoculation
Use automatic vaccination machine is inoculated, and bacterial classification is the hypsizygus marmoreus excellent species buying DSMZ of Shanghai academy of agricultural sciences, and inoculum concentration is 5% (v/v).
Cultivate:
Then room temperature 23 DEG C-25 DEG C is placed in, relative moisture 60%-70, C0
290d is cultivated under concentration 0.02%-0.025%.
Mycelium stimulation:
Use automatic mycelium stimulation machine after 90d, mycelium stimulation under normal temperature, normal pressure, suitable moisturizing during mycelium stimulation, the amount of moisturizing is being 15-20mL.
Fruiting:
Computer environment control system is used automatically to control fruiting: mycelia convalescence (1-5 days) the suitableeest parameter is temperature 16 DEG C, humidity 95-100%, light dark, C0
2concentration 0.1-0.2%, open air exhaust 5/20min, Inner eycle 3/20min; The suitableeest parameter of former base Formation period (5-12 days) is temperature 15 DEG C, humidity 90-95%, illumination 5/120min, C0
2concentration 0.1%, open air exhaust 30/lOmin, Inner eycle 15/10min; The suitableeest parameter of former base idiophase (12-14 days) is temperature 15 DEG C, humidity 90-95%, illumination 5/20-15/105min, C0
2concentration 0.1%, open air exhaust 10/15min, Inner eycle 15/10min; Sporophore growth phase (14-16 days) the suitableeest parameter is temperature 14.5 DEG C, humidity 95-100%, illumination 15/105-15/45min, C0
2concentration 0.1-0.2%, open air exhaust 10/15-15/l0min, Inner eycle 15/l0min; Fruit body maturing stage (16 days-gather) the suitableeest parameter is temperature 14.5-15.5 DEG C, humidity 95-100%, illumination 24h, C0
2concentration 0.1-0.2%, open air exhaust 15/l0min, Inner eycle 30/10-30/lmin.
Gather, pack:
Stem length 5-8cm, bacteria cover diameter 1.8-2.2cm, average mushroom clump height 5-6cm gathers, and packs.
it is characterized in that:
Other raw material 0.2-5% in Cultivar culture medium formula is hypsizygus marmoreus or true Ji mushroom pin handle powder or hypsizygus marmoreus or true Ji mushroom pin handle extract powder;
Hypsizygus marmoreus or true Ji mushroom pin handle powder are for be directly crushed to 20-40 order by hypsizygus marmoreus or true Ji mushroom pin handle;
Hypsizygus marmoreus or true Ji mushroom pin handle extract powder are extracted by following methods: hypsizygus marmoreus or true Ji mushroom pin handle are pulverized, add 5 times of water gagings, decoct 3 times, each decoction extraction 0.5 hour, merge decoction liquor, filter, filtrate is by processed good D101 non-polar macroporous resin, wherein the loading flow velocity of every 1m1 resin is between 1.20ml/h, described non-polar macroporous resin blade diameter length ratio is 1:10, after loading, the Static Adsorption time of filtrate is 1 hour, use water successively, the ethanol elution removing impurity of less than 18%, use 35% ethanol again, 0.5% sodium hydroxide wash-out, collect ethanol eluate, be evaporated to relative density is determined as 1.20 liquid extract at 60 DEG C, drying under reduced pressure, dry temperature is 50 DEG C, pulverize, obtain,
Water during spice is the aqueous solution of true Ji mushroom pin handle activity extract powder;
During mycelium stimulation, moisturizing 15-20mL is the aqueous solution of true Ji mushroom pin handle activity extract powder.
The invention has the beneficial effects as follows: the availability and the added value that add hypsizygus marmoreus pin handle resource, improve the Quality and yield of hypsizygus marmoreus factory culture.
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment
embodiment one
The present embodiment one of the present invention is a kind of hypsizygus marmoreus industrial planting method, the flow process of cultivation: raw-material examination---formula, spice---bottling---sterilizing---cooling,------gather, packaging in cultivation in inoculation by mycelium stimulation---fruiting---.
raw-material examination
Wood chip: (pH value: 7.2-7.7, water content: 60%-70%, granularity: below 2mm, silt content: below 3-5%, organoleptic indicator: leaf wood seeds, without going mouldy);
Cotton seed hulls: (pH value: 6.2-7.3, water content: less than 13%, below 3mm, less than 1%, cotton-wool is few, without going mouldy, insect);
Corncob: (pH value: 6.0-7.4, water content: less than 14%, below 3mm, less than 1%, nothing is gone mouldy, impurity, and dust is few);
Rice bran: (pH value: 6.2-7.4, water content: less than 14%, 4 mesh sieve percent of pass 100%, less than 1%, fresh, without going mouldy, becoming sour);
Corn flour: (pH value: 6.3-7.4, water content: less than 14%, 4 mesh sieve percent of pass 100%, less than 1%, fresh, without going mouldy, insect, to become sour);
Wheat bran: (pH value: 6.2-6.8, water content: less than 14%, below 2mm, less than 1%, dry, without going mouldy, becoming sour);
Calcium carbonate: (pH value: more than 11, water content: 4 mesh sieve percent of pass 100%, 0 are Powdered, without making moist, luming).
formula:
Cultivar culture medium formula is by weight percentage: deciduous species wood chip 22%, cotton seed hulls 11%, corncob 19%, rice bran 24%, corn flour 5%, wheat bran 13%, calcium carbonate 1%, other raw material 5%.
spice:
Sent into by above-mentioned Cultivar culture medium formulation material under normal temperature, normal pressure, Shanghai Ying Feng edible mushroom equipment Co., Ltd model is the automatic stirring machine of SY0102095, carries out spice.
bottling:
Above-mentioned spice being sent into Shanghai Ying Feng edible mushroom equipment Co., Ltd model under normal temperature, normal pressure is the charging of MRENG automatic bottling machine, 1100mL plastic bottle (70mm bore) is adopted to carry out bottle cultivation, every bottled wet feed 700g, water content 63%-65%, pH value is between 7.0-7.8.
sterilizing:
Above-mentioned bottle is planted material and send into the mushroom sterilizer sterilizing that Lianyungang Guoxin Pharmaceutical Equipment Co., Ltd.'s model is GXMQ-H, temperature 121 DEG C, high pressure 2Mpa, lower sterilizing 3h-4h.
cooling:
The planting material after bacterium of having gone out is positioned over cooling chamber cooling, is cooled to less than 22 degree, composts or fertilisers of cultivating could be pushed into transfer room inoculation.
inoculation:
Use the automatic vaccination machine of Japanese import solid spawn inoculation device model F-7000 to inoculate, bacterial classification is the hypsizygus marmoreus excellent species buying DSMZ of Shanghai academy of agricultural sciences, and inoculum concentration is 5% (v/v).
cultivate:
Then room temperature 23 DEG C-25 DEG C is placed in, relative moisture 60%-70, C0
290d is cultivated under concentration 0.02%-0.025%.
mycelium stimulation:
Use the automatic mycelium stimulation machine that Japanese import automatic mycelium stimulation machine model is STE after 90d, mycelium stimulation under normal temperature, normal pressure, suitable moisturizing during mycelium stimulation, the amount of moisturizing is best at 15-20mL.
fruiting:
Computer environment control system is used automatically to control fruiting: mycelia convalescence (1-5 days) the suitableeest parameter is temperature 16 DEG C, humidity 95-100%, light dark, C0
2concentration 0.1-0.2%, open air exhaust 5/20min, Inner eycle 3/20min; The suitableeest parameter of former base Formation period (5-12 days) is temperature 15 DEG C, humidity 90-95%, illumination 5/120min, C0
2concentration 0.1%, open air exhaust 30/lOmin, Inner eycle 15/10min; The suitableeest parameter of former base idiophase (12-14 days) is temperature 15 DEG C, humidity 90-95%, illumination 5/20-15/105min, C0
2concentration 0.1%, open air exhaust 10/15min, Inner eycle 15/10min; Sporophore growth phase (14-16 days) the suitableeest parameter is temperature 14.5 DEG C, humidity 95-100%, illumination 15/105-15/45min, C0
2concentration 0.1-0.2%, open air exhaust 10/15-15/l0min, Inner eycle 15/l0min; Fruit body maturing stage (16 days-gather) the suitableeest parameter is temperature 14.5-15.5 DEG C, humidity 95-100%, illumination 24h, C0
2concentration 0.1-0.2%, open air exhaust 15/l0min, Inner eycle 30/10-30/lmin.
gather, pack:
Stem length 5-8cm, bacteria cover diameter 1.8-2.2cm, average mushroom clump height 5-6cm gathers.
It is characterized in that:
Other raw material 5% in Cultivar culture medium formula, for being directly crushed to 20-40 order hypsizygus marmoreus or true Ji mushroom pin handle powder by hypsizygus marmoreus or true Ji mushroom pin handle;
Water during spice is the aqueous solution of true Ji mushroom pin handle activity extract powder;
During mycelium stimulation, moisturizing 15-20mL is the aqueous solution of true Ji mushroom pin handle activity extract powder.
embodiment two
The invention process two is a kind of hypsizygus marmoreus industrial planting methods, the flow process of cultivation: raw-material examination---formula, spice---bottling---sterilizing---cooling,------gather, packaging in cultivation in inoculation by mycelium stimulation---fruiting---.
raw-material examination
Wood chip: (pH value: 7.2-7.7, water content: 60%-70%, granularity: below 2mm, silt content: below 3-5%, organoleptic indicator: leaf wood seeds, without going mouldy);
Cotton seed hulls: (pH value: 6.2-7.3, water content: less than 13%, below 3mm, less than 1%, cotton-wool is few, without going mouldy, insect);
Corncob: (pH value: 6.0-7.4, water content: less than 14%, below 3mm, less than 1%, nothing is gone mouldy, impurity, and dust is few);
Rice bran: (pH value: 6.2-7.4, water content: less than 14%, 4 mesh sieve percent of pass 100%, less than 1%, fresh, without going mouldy, becoming sour);
Corn flour: (pH value: 6.3-7.4, water content: less than 14%, 4 mesh sieve percent of pass 100%, less than 1%, fresh, without going mouldy, insect, to become sour);
Wheat bran: (pH value: 6.2-6.8, water content: less than 14%, below 2mm, less than 1%, dry, without going mouldy, becoming sour);
Calcium carbonate: (pH value: more than 11, water content: 4 mesh sieve percent of pass 100%, 0 are Powdered, without making moist, luming).
formula:
Cultivar culture medium formula is by weight percentage: deciduous species wood chip 37%, cotton seed hulls 9%, corncob 17%, rice bran 22%, corn flour 3%, wheat bran 11%, calcium carbonate 0.8%, other raw material 0.2%;
spice:
Sent into by above-mentioned Cultivar culture medium formulation material under normal temperature, normal pressure, Shanghai Ying Feng edible mushroom equipment Co., Ltd model is the automatic stirring machine of SY0102095, carries out spice.
bottling:
Above-mentioned spice being sent into Shanghai Ying Feng edible mushroom equipment Co., Ltd model under normal temperature, normal pressure is the charging of MRENG automatic bottling machine, 1100mL plastic bottle (70mm bore) is adopted to carry out bottle cultivation, every bottled wet feed 700g, water content 63%-65%, pH value is between 7.0-7.8.
sterilizing:
Above-mentioned bottle is planted material and send into the mushroom sterilizer sterilizing that Lianyungang Guoxin Pharmaceutical Equipment Co., Ltd.'s model is GXMQ-H, temperature 121 DEG C, high pressure 2Mpa, lower sterilizing 3h-4h.
cooling:
The planting material after bacterium of having gone out is positioned over cooling chamber cooling, is cooled to less than 22 degree, composts or fertilisers of cultivating could be pushed into transfer room inoculation.
inoculation:
Use the automatic vaccination machine of Japanese import solid spawn inoculation device model F-7000 to inoculate, bacterial classification is the hypsizygus marmoreus excellent species buying DSMZ of Shanghai academy of agricultural sciences, and inoculum concentration is 5% (v/v).
cultivate:
Then room temperature 23 DEG C-25 DEG C is placed in, relative moisture 60%-70, C0
290d is cultivated under concentration 0.02%-0.025%.
mycelium stimulation:
Use the automatic mycelium stimulation machine that Japanese import automatic mycelium stimulation machine model is STE after 90d, mycelium stimulation under normal temperature, normal pressure, suitable moisturizing during mycelium stimulation, the amount of moisturizing is best at 15-20mL.
fruiting:
Computer environment control system is used automatically to control fruiting: mycelia convalescence (1-5 days) the suitableeest parameter is temperature 16 DEG C, humidity 95-100%, light dark, C0
2concentration 0.1-0.2%, open air exhaust 5/20min, Inner eycle 3/20min; The suitableeest parameter of former base Formation period (5-12 days) is temperature 15 DEG C, humidity 90-95%, illumination 5/120min, C0
2concentration 0.1%, open air exhaust 30/l0min, Inner eycle 15/10min; The suitableeest parameter of former base idiophase (12-14 days) is temperature 15 DEG C, humidity 90-95%, illumination 5/20-15/105min, C0
2concentration 0.1%, open air exhaust 10/15min, Inner eycle 15/10min; Sporophore growth phase (14-16 days) the suitableeest parameter is temperature 14.5 DEG C, humidity 95-100%, illumination 15/105-15/45min, C0
2concentration 0.1-0.2%, open air exhaust 10/15-15/l0min, Inner eycle 15/lOmin; Fruit body maturing stage (16 days-gather) the suitableeest parameter is temperature 14.5-15.5 DEG C, humidity 95-100%, illumination 24h, C0
2concentration 0.1-0.2%, open air exhaust 15/l0min, Inner eycle 30/10-30/lmin.
gather, pack:
Stem length 5-8cm, bacteria cover diameter 1.8-2.2cm, average mushroom clump height 5-6cm gathers.
It is characterized in that:
Other raw material 0.2% in Cultivar culture medium formula are hypsizygus marmoreus or true Ji mushroom pin handle activity extract powder.
Hypsizygus marmoreus or true Ji mushroom pin handle activity extract powder are extracted by following methods: hypsizygus marmoreus or true Ji mushroom pin handle are pulverized, add 5 times of water gagings, decoct 3 times, each decoction extraction 0.5 hour, merge decoction liquor, filter, filtrate is by processed good D101 non-polar macroporous resin, wherein the loading flow velocity of every 1m1 resin is between 1.20ml/h, described non-polar macroporous resin blade diameter length ratio is 1:10, after loading, the Static Adsorption time of filtrate is 1 hour, use water successively, the ethanol elution removing impurity of less than 18%, use 35% ethanol again, 0.5% sodium hydroxide wash-out, collect ethanol eluate, be evaporated to relative density is determined as 1.20 liquid extract at 60 DEG C, drying under reduced pressure, dry temperature is 50 DEG C, pulverize, obtain.
Water during spice is the aqueous solution of true Ji mushroom pin handle activity extract powder.
During mycelium stimulation, moisturizing 15-20mL is the aqueous solution of true Ji mushroom pin handle activity extract powder.
embodiment three
One hypsizygus marmoreus industrial planting method, the flow process of cultivation: raw-material examination---formula, spice---bottling---sterilizing---cooling,------gather, packaging in cultivation in inoculation by mycelium stimulation---fruiting---.
Raw-material examination:
Wood chip: (pH value: 7.2-7.7, water content: 60%-70%, granularity: below 2mm, silt content: below 3-5%, organoleptic indicator: leaf wood seeds, without going mouldy);
Cotton seed hulls: (pH value: 6.2-7.3, water content: less than 13%, below 3mm, less than 1%, cotton-wool is few, without going mouldy, insect);
Corncob: (pH value: 6.0-7.4, water content: less than 14%, below 3mm, less than 1%, nothing is gone mouldy, impurity, and dust is few);
Rice bran: (pH value: 6.2-7.4, water content: less than 14%, 4 mesh sieve percent of pass 100%, less than 1%, fresh, without going mouldy, becoming sour);
Corn flour: (pH value: 6.3-7.4, water content: less than 14%, 4 mesh sieve percent of pass 100%, less than 1%, fresh, without going mouldy, insect, to become sour);
Wheat bran: (pH value: 6.2-6.8, water content: less than 14%, below 2mm, less than 1%, dry, without going mouldy, becoming sour);
Calcium carbonate: (pH value: more than 11, water content: 4 mesh sieve percent of pass 100%, 0 are Powdered, without making moist, luming).
Formula:
Cultivar culture medium formula is by weight percentage: deciduous species wood chip 27%, cotton seed hulls 10%, corncob 18%, rice bran 23%, corn flour 4%, wheat bran 12%, calcium carbonate 1%, other raw material 5%.
Spice:
Under normal temperature, normal pressure, above-mentioned Cultivar culture medium formulation material is sent into automatic stirring machine, carry out spice.
Bottling:
Under normal temperature, normal pressure, above-mentioned spice is sent into automatic bottling machine charging, adopt 1100mL plastic bottle (70mm bore) to carry out bottle cultivation, every bottled wet feed 700g, water content 63%-65%, pH value is between 7.0-7.8.
Sterilizing:
Above-mentioned bottle is planted material and sends into sterilizer sterilizing, temperature 121 DEG C, high pressure 2Mpa, lower sterilizing 3h-4h.
Cooling:
The planting material after bacterium of having gone out is positioned over cooling chamber cooling, is cooled to less than 22 degree, composts or fertilisers of cultivating could be pushed into transfer room inoculation.
Inoculation:
Use automatic vaccination machine is inoculated, and bacterial classification is the hypsizygus marmoreus excellent species buying DSMZ of Shanghai academy of agricultural sciences, and inoculum concentration is 5% (v/v).
Cultivate:
Then room temperature 23 DEG C-25 DEG C is placed in, relative moisture 60%-70, C0
290d is cultivated under concentration 0.02%-0.025%.
Mycelium stimulation:
Use automatic mycelium stimulation machine after 90d, mycelium stimulation under normal temperature, normal pressure, suitable moisturizing during mycelium stimulation, the amount of moisturizing is being 15-20mL.
Fruiting:
Computer environment control system is used automatically to control fruiting: mycelia convalescence (1-5 days) the suitableeest parameter is temperature 16 DEG C, humidity 95-100%, light dark, C0
2concentration 0.1-0.2%, open air exhaust 5/20min, Inner eycle 3/20min; The suitableeest parameter of former base Formation period (5-12 days) is temperature 15 DEG C, humidity 90-95%, illumination 5/120min, C0
2concentration 0.1%, open air exhaust 30/lOmin, Inner eycle 15/10min; The suitableeest parameter of former base idiophase (12-14 days) is temperature 15 DEG C, humidity 90-95%, illumination 5/20-15/105min, C0
2concentration 0.1%, open air exhaust 10/15min, Inner eycle 15/10min; Sporophore growth phase (14-16 days) the suitableeest parameter is temperature 14.5 DEG C, humidity 95-100%, illumination 15/105-15/45min, C0
2concentration 0.1-0.2%, open air exhaust 10/15-15/l0min, Inner eycle 15/l0min; Fruit body maturing stage (16 days-gather) the suitableeest parameter is temperature 14.5-15.5 DEG C, humidity 95-100%, illumination 24h, C0
2concentration 0.1-0.2%, open air exhaust 15/l0min, Inner eycle 30/10-30/lmin.
Gather, pack:
Stem length 5-8cm, bacteria cover diameter 1.8-2.2cm, average mushroom clump height 5-6cm gathers, and packs.
it is characterized in that:
Other raw material 5% in Cultivar culture medium formula are hypsizygus marmoreus or true Ji mushroom pin handle powder 4.8% and hypsizygus marmoreus or true Ji mushroom pin handle extract powder 0.2%;
Wherein hypsizygus marmoreus or true Ji mushroom pin handle powder are for be directly crushed to 20-40 order by hypsizygus marmoreus or true Ji mushroom pin handle;
Hypsizygus marmoreus or true Ji mushroom pin handle extract powder are extracted by following methods: hypsizygus marmoreus or true Ji mushroom pin handle are pulverized, add 5 times of water gagings, decoct 3 times, each decoction extraction 0.5 hour, merge decoction liquor, filter, filtrate is by processed good D101 non-polar macroporous resin, wherein the loading flow velocity of every 1m1 resin is between 1.20ml/h, described non-polar macroporous resin blade diameter length ratio is 1:10, after loading, the Static Adsorption time of filtrate is 1 hour, use water successively, the ethanol elution removing impurity of less than 18%, use 35% ethanol again, 0.5% sodium hydroxide wash-out, collect ethanol eluate, be evaporated to relative density is determined as 1.20 liquid extract at 60 DEG C, drying under reduced pressure, dry temperature is 50 DEG C, pulverize, obtain.
Water during spice is the aqueous solution of true Ji mushroom pin handle activity extract powder.
During mycelium stimulation, moisturizing 15-20mL is the aqueous solution of true Ji mushroom pin handle activity extract powder.
Claims (1)
1. a hypsizygus marmoreus industrial planting method, the flow process of cultivation is: raw-material examination---formula, spice---bottling---sterilizing---cooling,------mycelium stimulation---fruiting---is gathered, packaging in cultivation in inoculation;
Raw-material examination:
Wood chip: (pH value: 7.2-7.7, water content: 60%-70%, granularity: below 2mm, silt content: below 3-5%, organoleptic indicator: leaf wood seeds, without going mouldy);
Cotton seed hulls: (pH value: 6.2-7.3, water content: less than 13%, below 3mm, less than 1%, cotton-wool is few, without going mouldy, insect);
Corncob: (pH value: 6.0-7.4, water content: less than 14%, below 3mm, less than 1%, nothing is gone mouldy, impurity, and dust is few);
Rice bran: (pH value: 6.2-7.4, water content: less than 14%, 4 mesh sieve percent of pass 100%, less than 1%, fresh, without going mouldy, becoming sour);
Corn flour: (pH value: 6.3-7.4, water content: less than 14%, 4 mesh sieve percent of pass 100%, less than 1%, fresh, without going mouldy, insect, to become sour);
Wheat bran: (pH value: 6.2-6.8, water content: less than 14%, below 2mm, less than 1%, dry, without going mouldy, becoming sour);
Calcium carbonate: (pH value: more than 11, water content: 4 mesh sieve percent of pass 100%, 0 are Powdered, without making moist, luming);
Formula:
Cultivar culture medium formula is by weight percentage: deciduous species wood chip 22-37%, cotton seed hulls 9-11%, corncob 17-19%, rice bran 22-24%, corn flour 3-5%, wheat bran 11-13%, calcium carbonate 0.8-1%, other raw material 0.2-5%;
Spice:
Under normal temperature, normal pressure, above-mentioned Cultivar culture medium formulation material is sent into automatic stirring machine, carry out spice;
Bottling:
Under normal temperature, normal pressure, above-mentioned spice is sent into automatic bottling machine charging, adopt 1100mL plastic bottle (70mm bore) to carry out bottle cultivation, every bottled wet feed 700g, water content 63%-65%, pH value is between 7.0-7.8;
Sterilizing:
Above-mentioned bottle is planted material and sends into sterilizer sterilizing, temperature 121 DEG C, high pressure 2Mpa, lower sterilizing 3h-4h;
Cooling:
The planting material after bacterium of having gone out is positioned over cooling chamber cooling, is cooled to less than 22 DEG C, composts or fertilisers of cultivating could be pushed into transfer room inoculation;
Inoculation:
Use automatic vaccination machine is inoculated, and bacterial classification is the hypsizygus marmoreus excellent species buying DSMZ of Shanghai academy of agricultural sciences, and inoculum concentration is 5% (v/v);
Cultivate:
Then room temperature 23 DEG C-25 DEG C is placed in, relative moisture 60%-70, C0
290d is cultivated under concentration 0.02%-0.025%;
Mycelium stimulation:
Use automatic mycelium stimulation machine after 90d, mycelium stimulation under normal temperature, normal pressure, suitable moisturizing during mycelium stimulation, the amount of moisturizing is being 15-20mL;
Fruiting:
Computer environment control system is used automatically to control fruiting: mycelia convalescence (1-5 days) the suitableeest parameter is temperature 16 DEG C, humidity 95-100%, light dark, C0
2concentration 0.1-0.2%, open air exhaust 5/20min, Inner eycle 3/20min; The suitableeest parameter of former base Formation period (5-12 days) is temperature 15 DEG C, humidity 90-95%, illumination 5/120min, C0
2concentration 0.1%, open air exhaust 30/l0min, Inner eycle 15/10min; The suitableeest parameter of former base idiophase (12-14 days) is temperature 15 DEG C, humidity 90-95%, illumination 5/20-15/105min, C0
2concentration 0.1%, open air exhaust 10/15min, Inner eycle 15/10min; Sporophore growth phase (14-16 days) the suitableeest parameter is temperature 14.5 DEG C, humidity 95-100%, illumination 15/105-15/45min, C0
2concentration 0.1-0.2%, open air exhaust 10/15-15/l0min, Inner eycle 15/l0min; Fruit body maturing stage (16 days-gather) the suitableeest parameter is temperature 14.5-15.5 DEG C, humidity 95-100%, illumination 24h, C0
2concentration 0.1-0.2%, open air exhaust 15/lOmin, Inner eycle 30/10-30/lmin;
Gather, pack:
Stem length 5-8cm, bacteria cover diameter 1.8-2.2cm, average mushroom clump height 5-6cm gathers, and packs;
it is characterized in that:
Other raw material 0.2-5% in Cultivar culture medium formula is hypsizygus marmoreus or true Ji mushroom pin handle powder or active hypsizygus marmoreus or true Ji mushroom pin handle extract powder;
Hypsizygus marmoreus or true Ji mushroom pin handle powder are for be directly crushed to 20-40 order by hypsizygus marmoreus or true Ji mushroom pin handle;
Hypsizygus marmoreus or true Ji mushroom pin handle activity extract powder are extracted by following methods: hypsizygus marmoreus or true Ji mushroom pin handle are pulverized, add 5 times of water gagings, decoct 3 times, each decoction extraction 0.5 hour, merge decoction liquor, filter, filtrate is by processed good D101 non-polar macroporous resin, wherein the loading flow velocity of every 1m1 resin is between 1.20ml/h, described non-polar macroporous resin blade diameter length ratio is 1:10, after loading, the Static Adsorption time of filtrate is 1 hour, use water successively, the ethanol elution removing impurity of less than 18%, use 35% ethanol again, 0.5% sodium hydroxide wash-out, collect ethanol eluate, be evaporated to relative density is determined as 1.20 liquid extract at 60 DEG C, drying under reduced pressure, dry temperature is 50 DEG C, pulverize, obtain.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107624504A (en) * | 2017-11-07 | 2018-01-26 | 福建万辰生物科技股份有限公司 | A kind of crab flavour mushroom cultural method |
| CN107996284A (en) * | 2017-12-28 | 2018-05-08 | 山东常生源菌业有限公司 | A kind of seafood mushroom production method |
| CN110521491A (en) * | 2019-10-10 | 2019-12-03 | 西充星河生物科技有限公司 | A kind of true pleurotus cornucopiae High-quality Cultivation method |
| CN112243797A (en) * | 2020-11-13 | 2021-01-22 | 贵州光明临港九道菇生物科技有限公司 | Industrial cultivation method for hypsizigus marmoreus |
| CN112779165A (en) * | 2019-11-08 | 2021-05-11 | 天津农学院 | Mushroom foot powder preservation culture medium for hypsizigus marmoreus strains and preparation method of mushroom foot powder preservation culture medium |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101455160A (en) * | 2007-12-12 | 2009-06-17 | 辽宁东方农业科技有限公司 | Bottle-cultivated edible fungus industrialized fungus house, device arrangement and production technique thereof |
| KR100965722B1 (en) * | 2009-04-24 | 2010-06-24 | 농업회사법인주식회사 뜰아채 | Mushroom culture, preparation methods of the same, and the functional foods comprising the same |
| CN103194323A (en) * | 2013-04-14 | 2013-07-10 | 周翔 | Washing powder for washing dishes by hands and preparation method of washing powder |
| CN104641931A (en) * | 2013-11-25 | 2015-05-27 | 东莞香市菌业科技有限公司 | Method for applying cedar sawdust flammulina velutipes culture medium waste in agrocybe cylindracea cultivation |
| CN104774084A (en) * | 2015-03-22 | 2015-07-15 | 邬金飞 | Manufacturing method of hypsizygus marmoreus cultivation material |
-
2016
- 2016-01-08 CN CN201610010557.2A patent/CN105493897A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101455160A (en) * | 2007-12-12 | 2009-06-17 | 辽宁东方农业科技有限公司 | Bottle-cultivated edible fungus industrialized fungus house, device arrangement and production technique thereof |
| KR100965722B1 (en) * | 2009-04-24 | 2010-06-24 | 농업회사법인주식회사 뜰아채 | Mushroom culture, preparation methods of the same, and the functional foods comprising the same |
| CN103194323A (en) * | 2013-04-14 | 2013-07-10 | 周翔 | Washing powder for washing dishes by hands and preparation method of washing powder |
| CN104641931A (en) * | 2013-11-25 | 2015-05-27 | 东莞香市菌业科技有限公司 | Method for applying cedar sawdust flammulina velutipes culture medium waste in agrocybe cylindracea cultivation |
| CN104774084A (en) * | 2015-03-22 | 2015-07-15 | 邬金飞 | Manufacturing method of hypsizygus marmoreus cultivation material |
Non-Patent Citations (1)
| Title |
|---|
| 王迎鑫: "蟹味菇（Hypsizygus marmorous）工厂化栽培稳定性研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107624504A (en) * | 2017-11-07 | 2018-01-26 | 福建万辰生物科技股份有限公司 | A kind of crab flavour mushroom cultural method |
| CN107996284A (en) * | 2017-12-28 | 2018-05-08 | 山东常生源菌业有限公司 | A kind of seafood mushroom production method |
| CN110521491A (en) * | 2019-10-10 | 2019-12-03 | 西充星河生物科技有限公司 | A kind of true pleurotus cornucopiae High-quality Cultivation method |
| CN112779165A (en) * | 2019-11-08 | 2021-05-11 | 天津农学院 | Mushroom foot powder preservation culture medium for hypsizigus marmoreus strains and preparation method of mushroom foot powder preservation culture medium |
| CN112779165B (en) * | 2019-11-08 | 2022-09-16 | 天津农学院 | Mushroom foot powder preservation medium for hypsizigus marmoreus strains and preparation method of mushroom foot powder preservation medium |
| CN112243797A (en) * | 2020-11-13 | 2021-01-22 | 贵州光明临港九道菇生物科技有限公司 | Industrial cultivation method for hypsizigus marmoreus |
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