CN102668878B - Method for cultivating flammulina velutipes by using aquilaria-sinensis-chip edible mushroom culture medium - Google Patents
Method for cultivating flammulina velutipes by using aquilaria-sinensis-chip edible mushroom culture medium Download PDFInfo
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Abstract
The invention relates to the technical field of cultivation of flammulina velutipes, and particularly to a method for cultivating flammulina velutipes by using an aquilaria-sinensis-chip edible mushroom culture medium. The aquilaria-sinensis-chip edible mushroom culture medium used in the method is composed of the following raw materials in percentages by weight: 10-60% of aquilaria sinensis chips, 10-35% of rice bran, 0-60% of corncobs, 0-20% of cornmeal, 0-20% of cottonseed hulls, 0.5-1.5% of calcium carbonate, and 0.5-1% of quicklime. The method for cultivating flammulina velutipes by using the aquilaria-sinensis-chip edible mushroom culture medium comprises the following steps of: a, raw material treatment: accumulating the aquilaria sinensis chips outdoors for more than three months, wherein the aquilaria sinensis chips keeps wet by being regularly watered during the accumulation process; b, blending; c, bottling; d, sterilizing; e, inoculating; f, mycelium culture; g, mycelium stimulation; and h, fruiting culture management. The flammulina velutipes cultivated by the method disclosed by the invention is good in quality, high in productivity, high in single yield, high in biotransformation rate, rich in nutritive ingredients, very fragrant, sweet, fresh and tender, and has a healthcare function and a medicinal value.
Description
Technical field
The present invention relates to golden mushroom plantation technical field, be specifically related to the method with the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush.
Background technology
In the conventional formulation that Asparagus is cultivated, all can use wood chip, but the wood chip kind using is more single, mainly take coniferals wood chips such as pine tree wood, Chinese fir wood as main.But the coniferals wood chips such as pine tree wood, Chinese fir wood contain a large amount of greases and aromatic substance, wherein, aromatic substance is noxious material, and grease and aromatic substance can seriously hinder hypha of edible fungus growth and fruit body development.If without ungrease treatment, this class Coniferous sawdust is the raw material that can not directly be used as culture medium of edible fungus.In prior art, Coniferous sawdust is carried out to ungrease treatment, generally need by the method for boiling, distillation, its technique is numerous and diverse, and cost is high, and effect is also bad.
In recent years, there are many places in conjunction with the miscellaneous resources advantage of local broadleaf tree, explore the Varieties for culture medium of edible fungus wood chip actively, according to domestic authoritative edible mushroom bibliographical information, developed successively the multiple deciduous species such as mulberry tree wood, rubber trees, birchwood, willow wood.For the raw material as culture medium of edible fungus, hardwood sawdust, compared with Coniferous sawdust, has good value.
The fragrant tree of tabernaemontanus bulrush is unique deciduous species with the local name in Dongguan in Chinese trees, has the plantation history of more than 1000 year in Dongguan, has deep history culture inside information.At present, Dongguan City, Guangdong Province is planted the fragrant tree of tabernaemontanus bulrush in a large number, development tabernaemontanus bulrush incense culture industry, and the medical value of research promotion tabernaemontanus bulrush perfume (or spice) and health are worth, and have developed the series of products such as tabernaemontanus bulrush scented tea, incense drug, sesame oil, spices, perfume.Exceed hundred kinds take tabernaemontanus bulrush perfume (or spice) as the Chinese medicine of formula, tabernaemontanus bulrush perfume is to boil water can treat the scytitises such as pruitus, and burning the fragrant smog producing of tabernaemontanus bulrush can purify air, sterilizing.But, temporarily without pertinent literature report, the tabernaemontanus bulrush incense wood bits of the fragrant tree of tabernaemontanus bulrush in Dongguan growth are opened up to utilization.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art, method with the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush is provided, not only high, the quality better of productive rate, nutrient component are abundant to utilize Asparagus that method provided by the present invention plants, fragrant and sweet especially fresh and tender, and there is health care and medical value.Wherein, only have the tabernaemontanus bulrush incense wood bits of the fragrant tree of tabernaemontanus bulrush utilizing in Dongguan growth to carry out cultivating flammulina velutipes, just can reach above-mentioned technical purpose.
To achieve these goals, the present invention adopts following technical scheme:
With the method for the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush, it is to cultivate Asparagus with the fragrant woodflour edible fungus culture medium of tabernaemontanus bulrush, and the fragrant woodflour edible fungus culture medium of described tabernaemontanus bulrush is made up of the raw material of following percentage by weight:
Tabernaemontanus bulrush incense wood bits 10%-60%
Rice bran 10%-35%
Corncob 0%-60%
Corn flour 0%-20%
Cotton seed hulls 0%-20%
Calcium carbonate 0.5%-1.5%
Quicklime 0.5%-1%;
With the method for the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush, it comprises the following steps:
A, raw material processing: tabernaemontanus bulrush incense wood is considered outdoor accumulation 3 months ~ 12 month to be worth doing keep tabernaemontanus bulrush incense wood bits moist by regularly watering in banking process; That rice bran, corncob, corn flour, cotton seed hulls, calcium carbonate, quicklime require is fresh, do not lump, without going mouldy and without worm;
B, spice;
C, bottling;
D, sterilizing;
E, inoculation;
F, cultural hypha;
G, mycelium stimulation;
H, mushroom producing culture management.
Concrete, with the method for the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush, it comprises the following steps:
A, raw material processing: tabernaemontanus bulrush incense wood is considered outdoor accumulation to be worth doing more than 3 months keep tabernaemontanus bulrush incense wood bits moist by regularly watering in banking process; That rice bran, corncob, corn flour, cotton seed hulls, calcium carbonate, quicklime require is fresh, do not lump, without going mouldy and without worm;
B, spice: according to above-mentioned formula ratio raw materials weighing, described raw material is placed in to agitator, first does and stir 15 minutes ~ 30 minutes, then adding water wets stirs 15 minutes ~ 30 minutes, obtains composts or fertilisers of cultivating;
C, bottling: described composts or fertilisers of cultivating is sent to bottling machine by pipeline, in culture bottle, is automatically packed into composts or fertilisers of cultivating from bottling machine; Then utilize perforator, the composts or fertilisers of cultivating in culture bottle is burrowed to the material end from charge level, make a call to altogether five holes, wherein, composts or fertilisers of cultivating center is a large hole, and large hole is uniformly distributed four duck eyes around; Then utilize capsuling machine to carry out automatic sealing to culture bottle;
D, sterilizing: after bottling capping, at once culture bottle is carried out to high steam moist heat sterilization, sterilising temp is 121 ℃ ~ 123 ℃, and sterilization time is 30 minutes ~ 90 minutes; After sterilizing finishes, treat that culture bottle is cooled to below 20 ℃, just can send into transfer room and inoculate;
E, inoculation: under aseptic condition, carry out machine automatization inoculation by inoculation device with the bacterial classification of choosing;
F, cultural hypha: after inoculation, send at once culturing room and cultivate; First 5 days of cultural hypha, is that mycelia recovers the stage, and mycelia respiratory capacity is less, and can ventilate 2～3 times every day to culturing room, each 15～30 minutes; Enter cultural hypha after the 6th day, mycelia respiratory capacity starts to increase gradually, and at this moment ventilation equipment is set to often open, and sends into continually fresh air, discharges indoor carbon dioxide; Latter 23～25 days of inoculation, mycelia is covered with whole composts or fertilisers of cultivating surface;
G, mycelium stimulation: after the mycelia of culture bottle is covered with, cultivate and finish, should carry out immediately mycelium stimulation; Described mycelium stimulation is exactly old bacterial classification piece and the mycoderma of automatically removing culture bottle the top 1cm～2cm by mushroom culturing device, to impel fruit body to occur simultaneously from media surface;
H, mushroom producing culture management: be divided into and urge flower bud stage, inhibition stage, encapsulate sheet stage, all educate stage, harvest stages.
In technique scheme, the moisture of the composts or fertilisers of cultivating obtaining in step b spice is controlled between 62% ~ 64%.
In technique scheme, in step b spice, the time of described dry stirring is 20 minutes, and the time of described wet stirring is 20 minutes.
In technique scheme, in step f cultural hypha, the temperature of described culturing room is controlled at 18 ℃ of left and right, and air humidity is controlled at below 75%, and lucifuge is cultivated.
In technique scheme, in step h mushroom producing culture management, described in urge the flower bud stage to be called again the stage of sprouting; Within first 7 days of mushroom producing culture process, in order to urge the flower bud stage, the indoor temperature of urging the flower bud stage is 13 ℃～16 ℃, and indoor humidity remains on more than 95%, and indoor carbon dioxide concentration is controlled at below 0.1%, and suitably ventilate every day.
In technique scheme, in the management of step h mushroom producing culture, the described inhibition stage is: in the time that mushroom bud grows to 1cm～2cm, mushroom producing culture enters into the inhibition stage; The indoor temperature in this stage is controlled at 4 ℃～6 ℃, and humidity remains on more than 85%, and gas concentration lwevel is controlled at below 0.1%, and ventilate to every day, and carries out illumination 1 h ~ 2h of 200LX every day.
In technique scheme, in the management of step h mushroom producing culture, the described encapsulate sheet stage is: in the time that mushroom body grows to high bottle outlet 2cm left and right, enter into the encapsulate sheet stage; This stage need be lived bottleneck with film circle, so that cultivation bottleneck top forms a small-sized gas concentration lwevel district, to impel the mushroom handle growth of Asparagus, suppresses the growth of mushroom cap, and Asparagus is grown by fan shape.
In technique scheme, in the management of step h mushroom producing culture, described indoor temperature of all educating the stage should be controlled at 6 ℃～8 ℃, and air humidity remains on more than 75%; Light, take dark as main, carries out the illumination of 300LX for every 3 days ~ 5 days, and each light application time is 30 minutes ~ 45 minutes.
In technique scheme, in step h mushroom producing culture management, described harvest stages is: grow to and enter harvest stages while exceeding film 1cm～2cm when the fruit body of Asparagus.
Compared with prior art, beneficial effect is in the present invention:
1) in the method with the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush provided by the invention, because the tabernaemontanus bulrush incense wood bits of the fragrant tree of tabernaemontanus bulrush with in Dongguan growth replace traditional wood chip, and tabernaemontanus bulrush incense wood bits can provide abundant cellulose, hemicellulose, lignin and special flavor component for the growth of Asparagus, thereby the Asparagus quality better that adopts method of the present invention to plant, fragrant and sweet especially fresh and tender, rounded and the homogeneous of mushroom cap, the diameter of mushroom cap is 5mm left and right, and mushroom cap there will not be " parachute-opening " phenomenon, mushroom body is as white as polished jade, and mushroom handle consolidation is not loose.
2) adopt the productive rate of the Asparagus that method of the present invention plants to increase, adopting effective radical of the Asparagus that traditional sawdust medium plants is out 200 ~ 400, adopting effective radical of the Asparagus that method of the present invention plants is 400 ~ 600, therefore, adopt the productive rate of the Asparagus that method of the present invention plants to increase nearly 1 times.
3) method with the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush provided by the invention, the single rate of the Asparagus planting is large, and the average single rate of the culture bottle of each 1200mL is 360g.
4) adopt the biological transformation ratio of the Asparagus that method of the present invention plants high, biological transformation ratio exceedes 110%; And adopt the biological transformation ratio of the Asparagus of traditional sawdust medium plantation to be generally 80%, therefore, the biological transformation ratio of the Asparagus that method of the present invention plants is 1.4 times of biological transformation ratio of the Asparagus of traditional sawdust medium plantation.
5) Asparagus that adopts method of the present invention to plant, fragrant and sweet tender and crisp, nutrient component is abundant, is especially rich in lysine and arginine, is of value to children's brain cell development, has good health care; And the Asparagus of cultivating contains proflavin, that proflavin has is anticancer, reducing blood lipid, food therapy function and the medical value such as protect the liver.
6) method with the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush of the present invention is simple.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
embodiment 1.
With the method for the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush, it is to cultivate Asparagus with the fragrant woodflour edible fungus culture medium of tabernaemontanus bulrush, and the fragrant woodflour edible fungus culture medium of tabernaemontanus bulrush is made up of following raw material: tabernaemontanus bulrush incense wood bits 35kg, rice bran 20kg, corncob 30 kg, corn flour 13 kg, calcium carbonate 1.5 kg, quicklime 0.5 kg.
With the method for the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush, it comprises the following steps:
A, raw material processing: tabernaemontanus bulrush incense wood is considered outdoor accumulation to be worth doing more than 3 months keep tabernaemontanus bulrush incense wood bits moist by regularly watering in banking process; That rice bran, corncob, corn flour, calcium carbonate, quicklime require is fresh, do not lump, without going mouldy and without worm;
B, spice: according to above-mentioned formula ratio raw materials weighing, raw material is placed in to agitator, first does and stir 20 minutes, then adding water wets stirs 20 minutes, obtains composts or fertilisers of cultivating, and wherein, the moisture of composts or fertilisers of cultivating is 62% of composts or fertilisers of cultivating total amount;
C, bottling: composts or fertilisers of cultivating is sent to bottling machine by pipeline, is automatically packed into composts or fertilisers of cultivating from bottling machine in culture bottle, wherein, the culture bottle of each 1200mL packs after composts or fertilisers of cultivating gross weight into, and to be that 860g(contains bottle heavy); Then utilize perforator, the composts or fertilisers of cultivating in culture bottle is burrowed to the material end from charge level, make a call to altogether five holes, wherein, composts or fertilisers of cultivating center is a large hole, and large hole is uniformly distributed four duck eyes around; Then utilize capsuling machine to carry out automatic sealing to culture bottle;
D, sterilizing: after bottling capping, at once culture bottle is carried out to high steam moist heat sterilization, sterilising temp is 121 ℃, and sterilization time is 50 minutes; After sterilizing finishes, treat that culture bottle is cooled to below 20 ℃, just can send into transfer room and inoculate;
E, inoculation: under aseptic condition, carry out machine automatization inoculation by inoculation device with the bacterial classification of choosing; In the present embodiment, 15 grams of bacterial classifications of the culture bottle of each 1200ml access, evenly receive composts or fertilisers of cultivating surface, wherein, before inoculation, the outside of seed bottle is disinfected in alcohol, and the old mycoderma of removing bacterial classification top re-use.
F, cultural hypha: after inoculation, send at once culturing room and cultivate, wherein, the temperature of culturing room is controlled at 18 ℃ of left and right, and air humidity is controlled at below 75%, and lucifuge is cultivated; First 5 days of cultural hypha, is that mycelia recovers the stage, and mycelia respiratory capacity is less, and can ventilate 2～3 times every day to culturing room, each 15～30 minutes; Enter cultural hypha after the 6th day, mycelia respiratory capacity starts to increase gradually, and at this moment ventilation equipment is set to often open, and sends into continually fresh air, discharges indoor carbon dioxide; Latter 23～25 days of inoculation, mycelia is covered with whole composts or fertilisers of cultivating surface;
G, mycelium stimulation: after the mycelia of culture bottle is covered with, cultivate and finish, should carry out immediately mycelium stimulation; Mycelium stimulation is exactly old bacterial classification piece and the mycoderma of automatically removing culture bottle the top 1cm by mushroom culturing device, to impel fruit body to occur simultaneously from media surface;
H, mushroom producing culture management: be divided into and urge flower bud stage, inhibition stage, encapsulate sheet stage, all educate stage, harvest stages.Wherein, whole mushroom producing culture administrative time is 30 days.
Concrete, the flower bud stage of urging of mushroom producing culture management is called again the stage of sprouting; Within first 7 days of mushroom producing culture process, in order to urge the flower bud stage, the indoor temperature of urging the flower bud stage is 13 ℃, and indoor humidity remains on more than 95%, and indoor carbon dioxide concentration is controlled at below 0.1%, and suitably ventilate every day.In the present embodiment, carry out humidification with ultrasonic humidifier.
Concrete, the inhibition stage of mushroom producing culture management is: in the time that mushroom bud grows to 1cm, mushroom producing culture enters into the inhibition stage; The indoor temperature in this stage is controlled at 4 ℃, and humidity remains on more than 85%, and gas concentration lwevel is controlled at below 0.1%, and ventilate to every day, and carries out illumination 1 h of 200LX every day.In the inhibition stage, though mushroom body poor growth is very neat, strong, strong.Wherein, the inhibition stage generally needs about 10 days.
Concrete, the encapsulate sheet stage of mushroom producing culture management is: in the time that mushroom body grows to high bottle outlet 2cm left and right, enter into the encapsulate sheet stage; This stage need be lived bottleneck with film circle, so that cultivation bottleneck top forms a small-sized gas concentration lwevel district, to impel the mushroom handle growth of Asparagus, suppresses the growth of mushroom cap, and Asparagus is grown by fan shape.
Concrete, the indoor temperature in the stage of all educating of mushroom producing culture management should be controlled at 6 ℃, and air humidity remains on more than 75%; Light is take dark as main, every illumination of carrying out 300LX for 3 days, and each light application time is 30 minutes.
Concrete, the harvest stages of mushroom producing culture management is: grow to and enter harvest stages while exceeding film 1cm when the fruit body of Asparagus.
Wherein, the Asparagus after gathering, packs after need cutting old root in time.Culture bottle after having gathered, the unified culturing room that pulls out, delivers to Wa Ping district, by automatic scratching machine, used medium in culture bottle is dug totally, after empty culture bottle is collected, delivers to bottling district, enters next time and produces.In addition, owing to being rich in plant growth nutrition in the medium using later, can carry out the collection of mushroom slag, and mushroom slag is delivered to fertilizer plant be processed into plant organic bio-fertilizer.
Wherein, tabernaemontanus bulrush incense wood bits mainly play the effect of moisturizing and ventilation in culture medium prescription, for Growth of Flammulina Velutipes provides cellulose, hemicellulose and lignin; Rice bran contains Growth of Flammulina Velutipes and grows necessary whole nutrients, for Asparagus provides crude protein and nitrogen free extract, adjusts the required C/N ratio of Growth of Flammulina Velutipes; Corncob mainly provides carbon source, raw fiber and nitrogen free extract, and has the effect of water suction, water conservation and ventilation; The growth that corn flour is Asparagus provides crude protein, nitrogen free extract, raw fiber etc.; Calcium carbonate and quicklime are mainly used in regulating the pH value of medium, and provide calcium ion for the growth of Asparagus.
Wherein, the pressure in the high steam moist heat sterilization that the present invention mentions is 1Kg/cm
2~ 1.25Kg/cm
2.
embodiment 2.
With the method for the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush, it is to cultivate Asparagus with the fragrant woodflour edible fungus culture medium of tabernaemontanus bulrush, and the fragrant woodflour edible fungus culture medium of tabernaemontanus bulrush is made up of following raw material: tabernaemontanus bulrush incense wood bits 50kg, rice bran 33.5 kg, cotton seed hulls 15 kg, calcium carbonate 1 kg, quicklime 0.5 kg.
With the method for the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush, it comprises the following steps:
A, raw material processing: tabernaemontanus bulrush incense wood is considered outdoor accumulation to be worth doing more than 3 months keep tabernaemontanus bulrush incense wood bits moist by regularly watering in banking process; That rice bran, corncob, corn flour, calcium carbonate, quicklime require is fresh, do not lump, without going mouldy and without worm;
B, spice: according to above-mentioned formula ratio raw materials weighing, raw material is placed in to agitator, first does and stir 15 minutes, then adding water wets stirs 15 minutes, obtains composts or fertilisers of cultivating, and wherein, the moisture of composts or fertilisers of cultivating is 64% of composts or fertilisers of cultivating total amount;
C, bottling: composts or fertilisers of cultivating is sent to bottling machine by pipeline, is automatically packed into composts or fertilisers of cultivating from bottling machine in culture bottle, wherein, the culture bottle of each 1200mL packs after composts or fertilisers of cultivating gross weight into, and to be that 860g(contains bottle heavy); Then utilize perforator, the composts or fertilisers of cultivating in culture bottle is burrowed to the material end from charge level, make a call to altogether five holes, wherein, composts or fertilisers of cultivating center is a large hole, and large hole is uniformly distributed four duck eyes around; Then utilize capsuling machine to carry out automatic sealing to culture bottle;
D, sterilizing: after bottling capping, at once culture bottle is carried out to high steam moist heat sterilization, sterilising temp is 123 ℃, and sterilization time is 30 minutes; After sterilizing finishes, treat that culture bottle is cooled to below 20 ℃, just can send into transfer room and inoculate;
E, inoculation: under aseptic condition, carry out machine automatization inoculation by inoculation device with the bacterial classification of choosing; In the present embodiment, 20 grams of bacterial classifications of the culture bottle of each 1200ml access, evenly receive composts or fertilisers of cultivating surface, wherein, before inoculation, the outside of seed bottle is disinfected in alcohol, and the old mycoderma of removing bacterial classification top re-use.
F, cultural hypha: after inoculation, send at once culturing room and cultivate, wherein, the temperature of culturing room is controlled at 18 ℃ of left and right, and air humidity is controlled at below 75%, and lucifuge is cultivated; First 5 days of cultural hypha, is that mycelia recovers the stage, and mycelia respiratory capacity is less, and can ventilate 2～3 times every day to culturing room, each 15～30 minutes; Enter cultural hypha after the 6th day, mycelia respiratory capacity starts to increase gradually, and at this moment ventilation equipment is set to often open, and sends into continually fresh air, discharges indoor carbon dioxide; Latter 23～25 days of inoculation, mycelia is covered with whole composts or fertilisers of cultivating surface;
G, mycelium stimulation: after the mycelia of culture bottle is covered with, cultivate and finish, should carry out immediately mycelium stimulation; Mycelium stimulation is exactly old bacterial classification piece and the mycoderma of automatically removing culture bottle the top 2cm by mushroom culturing device, to impel fruit body to occur simultaneously from media surface;
H, mushroom producing culture management: be divided into and urge flower bud stage, inhibition stage, encapsulate sheet stage, all educate stage, harvest stages.Wherein, whole mushroom producing culture administrative time is 30 days.
Concrete, the flower bud stage of urging of mushroom producing culture management is called again the stage of sprouting; Within first 7 days of mushroom producing culture process, in order to urge the flower bud stage, the indoor temperature of urging the flower bud stage is 16 ℃, and indoor humidity remains on more than 95%, and indoor carbon dioxide concentration is controlled at below 0.1%, and suitably ventilate every day.In the present embodiment, carry out humidification with ultrasonic humidifier.
Concrete, the inhibition stage of mushroom producing culture management is: in the time that mushroom bud grows to 2cm, mushroom producing culture enters into the inhibition stage; The indoor temperature in this stage is controlled at 6 ℃, and humidity remains on more than 85%, and gas concentration lwevel is controlled at below 0.1%, and ventilate to every day, and carries out the illumination 2h of 200LX every day.In the inhibition stage, though mushroom body poor growth is very neat, strong, strong.Wherein, the inhibition stage generally needs about 10 days.
Concrete, the encapsulate sheet stage of mushroom producing culture management is: in the time that mushroom body grows to high bottle outlet 2cm left and right, enter into the encapsulate sheet stage; This stage need be lived bottleneck with film circle, so that cultivation bottleneck top forms a small-sized gas concentration lwevel district, to impel the mushroom handle growth of Asparagus, suppresses the growth of mushroom cap, and Asparagus is grown by fan shape.
Concrete, the indoor temperature in the stage of all educating of mushroom producing culture management should be controlled at 8 ℃, and air humidity remains on more than 75%; Light is take dark as main, every illumination of carrying out 300LX for 5 days, and each light application time is 45 minutes.
Concrete, the harvest stages of mushroom producing culture management is: grow to and enter harvest stages while exceeding film 2cm when the fruit body of Asparagus.
embodiment 3.
With the method for the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush, it is to cultivate Asparagus with the fragrant woodflour edible fungus culture medium of tabernaemontanus bulrush, and the fragrant woodflour edible fungus culture medium of tabernaemontanus bulrush is made up of following raw material: tabernaemontanus bulrush incense wood bits 25kg, rice bran 29 kg, corncob 45 kg, calcium carbonate 0.5 kg, quicklime 0.5 kg.
With the method for the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush, it comprises the following steps:
A, raw material processing: tabernaemontanus bulrush incense wood is considered outdoor accumulation to be worth doing more than 3 months keep tabernaemontanus bulrush incense wood bits moist by regularly watering in banking process; That rice bran, corncob, corn flour, calcium carbonate, quicklime require is fresh, do not lump, without going mouldy and without worm;
B, spice: according to above-mentioned formula ratio raw materials weighing, raw material is placed in to agitator, first does and stir 25 minutes, then adding water wets stirs 30 minutes, obtains composts or fertilisers of cultivating, and wherein, the moisture of composts or fertilisers of cultivating is 63% of composts or fertilisers of cultivating total amount;
C, bottling: composts or fertilisers of cultivating is sent to bottling machine by pipeline, is automatically packed into composts or fertilisers of cultivating from bottling machine in culture bottle, wherein, the culture bottle of each 1200mL packs after composts or fertilisers of cultivating gross weight into, and to be that 860g(contains bottle heavy); Then utilize perforator, the composts or fertilisers of cultivating in culture bottle is burrowed to the material end from charge level, make a call to altogether five holes, wherein, composts or fertilisers of cultivating center is a large hole, and large hole is uniformly distributed four duck eyes around; Then utilize capsuling machine to carry out automatic sealing to culture bottle;
D, sterilizing: after bottling capping, at once culture bottle is carried out to steam moist heat sterilization, sterilising temp is 122 ℃, and sterilization time is 60 minutes; After sterilizing finishes, treat that culture bottle is cooled to below 20 ℃, just can send into transfer room and inoculate;
E, inoculation: under aseptic condition, carry out machine automatization inoculation by inoculation device with the bacterial classification of choosing; In the present embodiment, 18 grams of bacterial classifications of the culture bottle of each 1200ml access, evenly receive composts or fertilisers of cultivating surface, wherein, before inoculation, the outside of seed bottle is disinfected in alcohol, and the old mycoderma of removing bacterial classification top re-use.
F, cultural hypha: after inoculation, send at once culturing room and cultivate, wherein, the temperature of culturing room is controlled at 18 ℃ of left and right, and air humidity is controlled at below 75%, and lucifuge is cultivated; First 5 days of cultural hypha, is that mycelia recovers the stage, and mycelia respiratory capacity is less, and can ventilate 2～3 times every day to culturing room, each 15～30 minutes; Enter cultural hypha after the 6th day, mycelia respiratory capacity starts to increase gradually, and at this moment ventilation equipment is set to often open, and sends into continually fresh air, discharges indoor carbon dioxide; Latter 23～25 days of inoculation, mycelia is covered with whole composts or fertilisers of cultivating surface;
G, mycelium stimulation: after the mycelia of culture bottle is covered with, cultivate and finish, should carry out immediately mycelium stimulation; Mycelium stimulation is exactly old bacterial classification piece and the mycoderma of automatically removing culture bottle the top 1.5cm by mushroom culturing device, to impel fruit body to occur simultaneously from media surface;
H, mushroom producing culture management: be divided into and urge flower bud stage, inhibition stage, encapsulate sheet stage, all educate stage, harvest stages.Wherein, whole mushroom producing culture administrative time is 30 days.
Concrete, the flower bud stage of urging of mushroom producing culture management is called again the stage of sprouting; Within first 7 days of mushroom producing culture process, in order to urge the flower bud stage, the indoor temperature of urging the flower bud stage is 15 ℃, and indoor humidity remains on more than 95%, and indoor carbon dioxide concentration is controlled at below 0.1%, and suitably ventilate every day.In the present embodiment, carry out humidification with ultrasonic humidifier.
Concrete, the inhibition stage of mushroom producing culture management is: in the time that mushroom bud grows to 1.5cm, mushroom producing culture enters into the inhibition stage; The indoor temperature in this stage is controlled at 5 ℃, and humidity remains on more than 85%, and gas concentration lwevel is controlled at below 0.1%, and ventilate to every day, and carries out the illumination 1.5h of 200LX every day.In the inhibition stage, though mushroom body poor growth is very neat, strong, strong.Wherein, the inhibition stage generally needs about 10 days.
Concrete, the encapsulate sheet stage of mushroom producing culture management is: in the time that mushroom body grows to high bottle outlet 2cm left and right, enter into the encapsulate sheet stage; This stage need be lived bottleneck with film circle, so that cultivation bottleneck top forms a small-sized gas concentration lwevel district, to impel the mushroom handle growth of Asparagus, suppresses the growth of mushroom cap, and Asparagus is grown by fan shape.
Concrete, the indoor temperature in the stage of all educating of mushroom producing culture management should be controlled at 7 ℃, and air humidity remains on more than 75%; Light is take dark as main, every illumination of carrying out 300LX for 4 days, and each light application time is 40 minutes.
Concrete, the harvest stages of mushroom producing culture management is: grow to and enter harvest stages while exceeding film 1.5cm when the fruit body of Asparagus.
embodiment 4.
With the method for the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush, it is to cultivate Asparagus with the fragrant woodflour edible fungus culture medium of tabernaemontanus bulrush, and the fragrant woodflour edible fungus culture medium of tabernaemontanus bulrush is made up of following raw material: tabernaemontanus bulrush incense wood bits 35kg, rice bran 18.7 kg, corncob 30 kg, corn flour 15 kg, calcium carbonate 0.8kg, quicklime 0.5 kg.
With the method for the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush, it comprises the following steps:
A, raw material processing: tabernaemontanus bulrush incense wood is considered outdoor accumulation to be worth doing more than 3 months keep tabernaemontanus bulrush incense wood bits moist by regularly watering in banking process; That rice bran, corncob, corn flour, calcium carbonate, quicklime require is fresh, do not lump, without going mouldy and without worm;
B, spice: according to above-mentioned formula ratio raw materials weighing, raw material is placed in to agitator, first does and stir 30 minutes, then adding water wets stirs 25 minutes, obtains composts or fertilisers of cultivating, and wherein, the moisture of composts or fertilisers of cultivating is 62.5% of composts or fertilisers of cultivating total amount;
C, bottling: composts or fertilisers of cultivating is sent to bottling machine by pipeline, is automatically packed into composts or fertilisers of cultivating from bottling machine in culture bottle, wherein, the culture bottle of each 1200mL packs after composts or fertilisers of cultivating gross weight into, and to be that 860g(contains bottle heavy); Then utilize perforator, the composts or fertilisers of cultivating in culture bottle is burrowed to the material end from charge level, make a call to altogether five holes, wherein, composts or fertilisers of cultivating center is a large hole, and large hole is uniformly distributed four duck eyes around; Then utilize capsuling machine to carry out automatic sealing to culture bottle;
D, sterilizing: after bottling capping, at once culture bottle is carried out to high steam moist heat sterilization, sterilising temp is 121.5 ℃, and sterilization time is 70 minutes; After sterilizing finishes, treat that culture bottle is cooled to below 20 ℃, just can send into transfer room and inoculate;
E, inoculation: under aseptic condition, carry out machine automatization inoculation by inoculation device with the bacterial classification of choosing; In the present embodiment, 16 grams of bacterial classifications of the culture bottle of each 1200ml access, evenly receive composts or fertilisers of cultivating surface, wherein, before inoculation, the outside of seed bottle is disinfected in alcohol, and the old mycoderma of removing bacterial classification top re-use.
F, cultural hypha: after inoculation, send at once culturing room and cultivate, wherein, the temperature of culturing room is controlled at 18 ℃ of left and right, and air humidity is controlled at below 75%, and lucifuge is cultivated; First 5 days of cultural hypha, is that mycelia recovers the stage, and mycelia respiratory capacity is less, and can ventilate 2～3 times every day to culturing room, each 15～30 minutes; Enter cultural hypha after the 6th day, mycelia respiratory capacity starts to increase gradually, and at this moment ventilation equipment is set to often open, and sends into continually fresh air, discharges indoor carbon dioxide; Latter 23～25 days of inoculation, mycelia is covered with whole composts or fertilisers of cultivating surface;
G, mycelium stimulation: after the mycelia of culture bottle is covered with, cultivate and finish, should carry out immediately mycelium stimulation; Mycelium stimulation is exactly old bacterial classification piece and the mycoderma of automatically removing culture bottle the top 1.8cm by mushroom culturing device, to impel fruit body to occur simultaneously from media surface;
H, mushroom producing culture management: be divided into and urge flower bud stage, inhibition stage, encapsulate sheet stage, all educate stage, harvest stages.Wherein, whole mushroom producing culture administrative time is 30 days.
Concrete, the flower bud stage of urging of mushroom producing culture management is called again the stage of sprouting; Within first 7 days of mushroom producing culture process, in order to urge the flower bud stage, the indoor temperature of urging the flower bud stage is 14 ℃, and indoor humidity remains on more than 95%, and indoor carbon dioxide concentration is controlled at below 0.1%, and suitably ventilate every day.In the present embodiment, carry out humidification with ultrasonic humidifier.
Concrete, the inhibition stage of mushroom producing culture management is: in the time that mushroom bud grows to 1.2cm, mushroom producing culture enters into the inhibition stage; The indoor temperature in this stage is controlled at 6 ℃, and humidity remains on more than 85%, and gas concentration lwevel is controlled at below 0.1%, and ventilate to every day, and carries out the illumination 1.2h of 200LX every day.In the inhibition stage, though mushroom body poor growth is very neat, strong, strong.Wherein, the inhibition stage generally needs about 10 days.
Concrete, the encapsulate sheet stage of mushroom producing culture management is: in the time that mushroom body grows to high bottle outlet 2cm left and right, enter into the encapsulate sheet stage; This stage need be lived bottleneck with film circle, so that cultivation bottleneck top forms a small-sized gas concentration lwevel district, to impel the mushroom handle growth of Asparagus, suppresses the growth of mushroom cap, and Asparagus is grown by fan shape.
Concrete, the indoor temperature in the stage of all educating of mushroom producing culture management should be controlled at 7 ℃, and air humidity remains on more than 75%; Light is take dark as main, every illumination of carrying out 300LX for 5 days, and each light application time is 35 minutes.
Concrete, the harvest stages of mushroom producing culture management is: grow to and enter harvest stages while exceeding film 1.8cm when the fruit body of Asparagus.
embodiment 5.
With the method for the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush, it is to cultivate Asparagus with the fragrant woodflour edible fungus culture medium of tabernaemontanus bulrush, and the fragrant woodflour edible fungus culture medium of tabernaemontanus bulrush is made up of following raw material: tabernaemontanus bulrush incense wood bits 50kg, rice bran 33 kg, cotton seed hulls 15 kg, calcium carbonate 1kg, quicklime 1 kg.
With the method for the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush, it comprises the following steps:
A, raw material processing: tabernaemontanus bulrush incense wood is considered outdoor accumulation to be worth doing more than 3 months keep tabernaemontanus bulrush incense wood bits moist by regularly watering in banking process; That rice bran, corncob, corn flour, calcium carbonate, quicklime require is fresh, do not lump, without going mouldy and without worm;
B, spice: according to above-mentioned formula ratio raw materials weighing, raw material is placed in to agitator, first does and stir 20 minutes, then adding water wets stirs 15 minutes, obtains composts or fertilisers of cultivating, and wherein, the moisture of composts or fertilisers of cultivating is 63.5% of composts or fertilisers of cultivating total amount;
C, bottling: composts or fertilisers of cultivating is sent to bottling machine by pipeline, is automatically packed into composts or fertilisers of cultivating from bottling machine in culture bottle, wherein, the culture bottle of each 1200mL packs after composts or fertilisers of cultivating gross weight into, and to be that 860g(contains bottle heavy); Then utilize perforator, the composts or fertilisers of cultivating in culture bottle is burrowed to the material end from charge level, make a call to altogether five holes, wherein, composts or fertilisers of cultivating center is a large hole, and large hole is uniformly distributed four duck eyes around; Then utilize capsuling machine to carry out automatic sealing to culture bottle;
D, sterilizing: after bottling capping, at once culture bottle is carried out to high steam moist heat sterilization, sterilising temp is 123 ℃, and sterilization time is 45 minutes; After sterilizing finishes, treat that culture bottle is cooled to below 20 ℃, just can send into transfer room and inoculate;
E, inoculation: under aseptic condition, carry out machine automatization inoculation by inoculation device with the bacterial classification of choosing; In the present embodiment, 19 grams of bacterial classifications of the culture bottle of each 1200ml access, evenly receive composts or fertilisers of cultivating surface, wherein, before inoculation, the outside of seed bottle is disinfected in alcohol, and the old mycoderma of removing bacterial classification top re-use.
F, cultural hypha: after inoculation, send at once culturing room and cultivate, wherein, the temperature of culturing room is controlled at 18 ℃ of left and right, and air humidity is controlled at below 75%, and lucifuge is cultivated; First 5 days of cultural hypha, is that mycelia recovers the stage, and mycelia respiratory capacity is less, and can ventilate 2～3 times every day to culturing room, each 15～30 minutes; Enter cultural hypha after the 6th day, mycelia respiratory capacity starts to increase gradually, and at this moment ventilation equipment is set to often open, and sends into continually fresh air, discharges indoor carbon dioxide; Latter 23～25 days of inoculation, mycelia is covered with whole composts or fertilisers of cultivating surface;
G, mycelium stimulation: after the mycelia of culture bottle is covered with, cultivate and finish, should carry out immediately mycelium stimulation; Mycelium stimulation is exactly old bacterial classification piece and the mycoderma of automatically removing culture bottle the top 1.6cm by mushroom culturing device, to impel fruit body to occur simultaneously from media surface;
H, mushroom producing culture management: be divided into and urge flower bud stage, inhibition stage, encapsulate sheet stage, all educate stage, harvest stages.Wherein, whole mushroom producing culture administrative time is 30 days.
Concrete, the flower bud stage of urging of mushroom producing culture management is called again the stage of sprouting; Within first 7 days of mushroom producing culture process, in order to urge the flower bud stage, the indoor temperature of urging the flower bud stage is 13 ℃, and indoor humidity remains on more than 95%, and indoor carbon dioxide concentration is controlled at below 0.1%, and suitably ventilate every day.In the present embodiment, carry out humidification with ultrasonic humidifier.
Concrete, the inhibition stage of mushroom producing culture management is: in the time that mushroom bud grows to 1.8cm, mushroom producing culture enters into the inhibition stage; The indoor temperature in this stage is controlled at 4 ℃, and humidity remains on more than 85%, and gas concentration lwevel is controlled at below 0.1%, and ventilate to every day, and carries out the illumination 2h of 200LX every day.In the inhibition stage, though mushroom body poor growth is very neat, strong, strong.Wherein, the inhibition stage generally needs about 10 days.
Concrete, the encapsulate sheet stage of mushroom producing culture management is: in the time that mushroom body grows to high bottle outlet 2cm left and right, enter into the encapsulate sheet stage; This stage need be lived bottleneck with film circle, so that cultivation bottleneck top forms a small-sized gas concentration lwevel district, to impel the mushroom handle growth of Asparagus, suppresses the growth of mushroom cap, and Asparagus is grown by fan shape.
Concrete, the indoor temperature in the stage of all educating of mushroom producing culture management should be controlled at 6 ℃, and air humidity remains on more than 75%; Light is take dark as main, every illumination of carrying out 300LX for 3 days, and each light application time is 40 minutes.
Concrete, the harvest stages of mushroom producing culture management is: grow to and enter harvest stages while exceeding film 1.2cm when the fruit body of Asparagus.
embodiment 6.
With the method for the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush, it is to cultivate Asparagus with the fragrant woodflour edible fungus culture medium of tabernaemontanus bulrush, and the fragrant woodflour edible fungus culture medium of tabernaemontanus bulrush is made up of following raw material: tabernaemontanus bulrush incense wood bits 25kg, rice bran 23 kg, corncob 50 kg, calcium carbonate 1kg, quicklime 1 kg.
With the method for the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush, it comprises the following steps:
A, raw material processing: tabernaemontanus bulrush incense wood is considered outdoor accumulation to be worth doing more than 3 months keep tabernaemontanus bulrush incense wood bits moist by regularly watering in banking process; That rice bran, corncob, corn flour, calcium carbonate, quicklime require is fresh, do not lump, without going mouldy and without worm;
B, spice: according to above-mentioned formula ratio raw materials weighing, raw material is placed in to agitator, first does and stir 15 minutes, then adding water wets stirs 20 minutes, obtains composts or fertilisers of cultivating, and wherein, the moisture of composts or fertilisers of cultivating is 64% of composts or fertilisers of cultivating total amount;
C, bottling: composts or fertilisers of cultivating is sent to bottling machine by pipeline, is automatically packed into composts or fertilisers of cultivating from bottling machine in culture bottle, wherein, the culture bottle of each 1200mL packs after composts or fertilisers of cultivating gross weight into, and to be that 860g(contains bottle heavy); Then utilize perforator, the composts or fertilisers of cultivating in culture bottle is burrowed to the material end from charge level, make a call to altogether five holes, wherein, composts or fertilisers of cultivating center is a large hole, and large hole is uniformly distributed four duck eyes around; Then utilize capsuling machine to carry out automatic sealing to culture bottle;
D, sterilizing: after bottling capping, at once culture bottle is carried out to high steam moist heat sterilization, sterilising temp is 122.5 ℃, and sterilization time is 90 minutes; After sterilizing finishes, treat that culture bottle is cooled to below 20 ℃, just can send into transfer room and inoculate;
E, inoculation: under aseptic condition, carry out machine automatization inoculation by inoculation device with the bacterial classification of choosing; In the present embodiment, 17 grams of bacterial classifications of the culture bottle of each 1200ml access, evenly receive composts or fertilisers of cultivating surface, wherein, before inoculation, the outside of seed bottle is disinfected in alcohol, and the old mycoderma of removing bacterial classification top re-use.
F, cultural hypha: after inoculation, send at once culturing room and cultivate, wherein, the temperature of culturing room is controlled at 18 ℃ of left and right, and air humidity is controlled at below 75%, and lucifuge is cultivated; First 5 days of cultural hypha, is that mycelia recovers the stage, and mycelia respiratory capacity is less, and can ventilate 2～3 times every day to culturing room, each 15～30 minutes; Enter cultural hypha after the 6th day, mycelia respiratory capacity starts to increase gradually, and at this moment ventilation equipment is set to often open, and sends into continually fresh air, discharges indoor carbon dioxide; Latter 23～25 days of inoculation, mycelia is covered with whole composts or fertilisers of cultivating surface;
G, mycelium stimulation: after the mycelia of culture bottle is covered with, cultivate and finish, should carry out immediately mycelium stimulation; Mycelium stimulation is exactly old bacterial classification piece and the mycoderma of automatically removing culture bottle the top 1.4cm by mushroom culturing device, to impel fruit body to occur simultaneously from media surface;
H, mushroom producing culture management: be divided into and urge flower bud stage, inhibition stage, encapsulate sheet stage, all educate stage, harvest stages.Wherein, whole mushroom producing culture administrative time is 30 days.
Concrete, the flower bud stage of urging of mushroom producing culture management is called again the stage of sprouting; Within first 7 days of mushroom producing culture process, in order to urge the flower bud stage, the indoor temperature of urging the flower bud stage is 13.5 ℃, and indoor humidity remains on more than 95%, and indoor carbon dioxide concentration is controlled at below 0.1%, and suitably ventilate every day.In the present embodiment, carry out humidification with ultrasonic humidifier.
Concrete, the inhibition stage of mushroom producing culture management is: in the time that mushroom bud grows to 1.3cm, mushroom producing culture enters into the inhibition stage; The indoor temperature in this stage is controlled at 5 ℃, and humidity remains on more than 85%, and gas concentration lwevel is controlled at below 0.1%, and ventilate to every day, and carries out the illumination 1.6h of 200LX every day.In the inhibition stage, though mushroom body poor growth is very neat, strong, strong.Wherein, the inhibition stage generally needs about 10 days.
Concrete, the encapsulate sheet stage of mushroom producing culture management is: in the time that mushroom body grows to high bottle outlet 2cm left and right, enter into the encapsulate sheet stage; This stage need be lived bottleneck with film circle, so that cultivation bottleneck top forms a small-sized gas concentration lwevel district, to impel the mushroom handle growth of Asparagus, suppresses the growth of mushroom cap, and Asparagus is grown by fan shape.
Concrete, the indoor temperature in the stage of all educating of mushroom producing culture management should be controlled at 8 ℃, and air humidity remains on more than 75%; Light is take dark as main, every illumination of carrying out 300LX for 4 days, and each light application time is 45 minutes.
Concrete, the harvest stages of mushroom producing culture management is: grow to and enter harvest stages while exceeding film 1.4cm when the fruit body of Asparagus.
embodiment 7.
With the method for the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush, it is to cultivate Asparagus with the fragrant woodflour edible fungus culture medium of tabernaemontanus bulrush, and the fragrant woodflour edible fungus culture medium of tabernaemontanus bulrush is made up of following raw material: tabernaemontanus bulrush incense wood bits 60kg, rice bran 10 kg, corn flour 20 kg, cotton seed hulls 8 kg, calcium carbonate 1.4kg, quicklime 0.6 kg.
With the method for the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush, it comprises the following steps:
A, raw material processing: tabernaemontanus bulrush incense wood is considered outdoor accumulation to be worth doing more than 3 months keep tabernaemontanus bulrush incense wood bits moist by regularly watering in banking process; That rice bran, corncob, corn flour, calcium carbonate, quicklime require is fresh, do not lump, without going mouldy and without worm;
B, spice: according to above-mentioned formula ratio raw materials weighing, raw material is placed in to agitator, first does and stir 20 minutes, then adding water wets stirs 26 minutes, obtains composts or fertilisers of cultivating, and wherein, the moisture of composts or fertilisers of cultivating is 63% of composts or fertilisers of cultivating total amount;
C, bottling: composts or fertilisers of cultivating is sent to bottling machine by pipeline, is automatically packed into composts or fertilisers of cultivating from bottling machine in culture bottle, wherein, the culture bottle of each 1200mL packs after composts or fertilisers of cultivating gross weight into, and to be that 860g(contains bottle heavy); Then utilize perforator, the composts or fertilisers of cultivating in culture bottle is burrowed to the material end from charge level, make a call to altogether five holes, wherein, composts or fertilisers of cultivating center is a large hole, and large hole is uniformly distributed four duck eyes around; Then utilize capsuling machine to carry out automatic sealing to culture bottle;
D, sterilizing: after bottling capping, at once culture bottle is carried out to high steam moist heat sterilization, sterilising temp is 121 ℃, and sterilization time is 55 minutes; After sterilizing finishes, treat that culture bottle is cooled to below 20 ℃, just can send into transfer room and inoculate;
E, inoculation: under aseptic condition, carry out machine automatization inoculation by inoculation device with the bacterial classification of choosing; In the present embodiment, 20 grams of bacterial classifications of the culture bottle of each 1200ml access, evenly receive composts or fertilisers of cultivating surface, wherein, before inoculation, the outside of seed bottle is disinfected in alcohol, and the old mycoderma of removing bacterial classification top re-use.
F, cultural hypha: after inoculation, send at once culturing room and cultivate, wherein, the temperature of culturing room is controlled at 18 ℃ of left and right, and air humidity is controlled at below 75%, and lucifuge is cultivated; First 5 days of cultural hypha, is that mycelia recovers the stage, and mycelia respiratory capacity is less, and can ventilate 2～3 times every day to culturing room, each 15～30 minutes; Enter cultural hypha after the 6th day, mycelia respiratory capacity starts to increase gradually, and at this moment ventilation equipment is set to often open, and sends into continually fresh air, discharges indoor carbon dioxide; Latter 23～25 days of inoculation, mycelia is covered with whole composts or fertilisers of cultivating surface;
G, mycelium stimulation: after the mycelia of culture bottle is covered with, cultivate and finish, should carry out immediately mycelium stimulation; Mycelium stimulation is exactly old bacterial classification piece and the mycoderma of automatically removing culture bottle the top 1.7cm by mushroom culturing device, to impel fruit body to occur simultaneously from media surface;
H, mushroom producing culture management: be divided into and urge flower bud stage, inhibition stage, encapsulate sheet stage, all educate stage, harvest stages.Wherein, whole mushroom producing culture administrative time is 30 days.
Concrete, the flower bud stage of urging of mushroom producing culture management is called again the stage of sprouting; Within first 7 days of mushroom producing culture process, in order to urge the flower bud stage, the indoor temperature of urging the flower bud stage is 15 ℃, and indoor humidity remains on more than 95%, and indoor carbon dioxide concentration is controlled at below 0.1%, and suitably ventilate every day.In the present embodiment, carry out humidification with ultrasonic humidifier.
Concrete, the inhibition stage of mushroom producing culture management is: in the time that mushroom bud grows to 1.7cm, mushroom producing culture enters into the inhibition stage; The indoor temperature in this stage is controlled at 4 ℃, and humidity remains on more than 85%, and gas concentration lwevel is controlled at below 0.1%, and ventilate to every day, and carries out the illumination 2h of 200LX every day.In the inhibition stage, though mushroom body poor growth is very neat, strong, strong.Wherein, the inhibition stage generally needs about 10 days.
Concrete, the encapsulate sheet stage of mushroom producing culture management is: in the time that mushroom body grows to high bottle outlet 2cm left and right, enter into the encapsulate sheet stage; This stage need be lived bottleneck with film circle, so that cultivation bottleneck top forms a small-sized gas concentration lwevel district, to impel the mushroom handle growth of Asparagus, suppresses the growth of mushroom cap, and Asparagus is grown by fan shape.
Concrete, the indoor temperature in the stage of all educating of mushroom producing culture management should be controlled at 6 ℃, and air humidity remains on more than 75%; Light is take dark as main, every illumination of carrying out 300LX for 5 days, and each light application time is 40 minutes.
Concrete, the harvest stages of mushroom producing culture management is: grow to and enter harvest stages while exceeding film 1.6cm when the fruit body of Asparagus.
Finally should be noted that; above embodiment is only in order to illustrate technical scheme of the present invention; but not limiting the scope of the invention; although the present invention has been done to explain with reference to preferred embodiment; those of ordinary skill in the art is to be understood that; can modify or be equal to replacement technical scheme of the present invention, and not depart from essence and the scope of technical solution of the present invention.
Claims (8)
1. with the method for the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush, it is characterized in that: it is to cultivate Asparagus with the fragrant woodflour edible fungus culture medium of tabernaemontanus bulrush, and the fragrant woodflour edible fungus culture medium of described tabernaemontanus bulrush is made up of the raw material of following percentage by weight:
Tabernaemontanus bulrush incense wood bits 10% ~ 25% or 50% ~ 60%
Rice bran 10% ~ 23% or 29% ~ 35%
Corncob 0%-60%
Corn flour 0% or 13% ~ 20%
Cotton seed hulls 0%-20%
Calcium carbonate 0.5% ~ 0.8% or 1.4% ~ 1.5%
Quicklime 0.5%-1%;
The content sum of each component is 100%;
With the method for the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush, it comprises the following steps:
A, raw material processing: tabernaemontanus bulrush incense wood is considered outdoor accumulation to be worth doing more than 3 months keep tabernaemontanus bulrush incense wood bits moist by regularly watering in banking process; That rice bran, corncob, corn flour, cotton seed hulls, calcium carbonate, quicklime require is fresh, do not lump, without going mouldy and without worm;
B, spice: according to above-mentioned formula ratio raw materials weighing, described raw material is placed in to agitator, first does and stir 15 minutes ~ 30 minutes, then adding water wets stirs 15 minutes ~ 30 minutes, obtains composts or fertilisers of cultivating;
C, bottling: described composts or fertilisers of cultivating is sent to bottling machine by pipeline, in culture bottle, is automatically packed into composts or fertilisers of cultivating from bottling machine; Then utilize perforator, the composts or fertilisers of cultivating in culture bottle is burrowed to the material end from charge level, make a call to altogether five holes, wherein, composts or fertilisers of cultivating center is a large hole, and large hole is uniformly distributed four duck eyes around; Then utilize capsuling machine to carry out automatic sealing to culture bottle;
D, sterilizing: after bottling capping, at once culture bottle is carried out to high steam moist heat sterilization, sterilising temp is 121 ℃ ~ 123 ℃, and sterilization time is 30 minutes ~ 90 minutes; After sterilizing finishes, treat that culture bottle is cooled to below 20 ℃, just can send into transfer room and inoculate;
E, inoculation: under aseptic condition, carry out machine automatization inoculation by inoculation device with the bacterial classification of choosing;
F, cultural hypha: after inoculation, send at once culturing room and cultivate; First 5 days of cultural hypha, is that mycelia recovers the stage, and mycelia respiratory capacity is less, and ventilate 2～3 times every day to culturing room, each 15～30 minutes; Enter cultural hypha after the 6th day, mycelia respiratory capacity starts to increase gradually, and at this moment ventilation equipment is set to often open, and sends into continually fresh air, discharges indoor carbon dioxide; Latter 23～25 days of inoculation, mycelia is covered with whole composts or fertilisers of cultivating surface; Wherein, the process of described cultural hypha is that lucifuge is cultivated;
G, mycelium stimulation: after the mycelia of culture bottle is covered with, cultivate and finish, should carry out immediately mycelium stimulation; Described mycelium stimulation is exactly old bacterial classification piece and the mycoderma of automatically removing culture bottle the top 1cm～2cm by mushroom culturing device, to impel fruit body to occur simultaneously from media surface;
H, mushroom producing culture management: be divided into and urge flower bud stage, inhibition stage, encapsulate sheet stage, all educate stage, harvest stages;
Describedly urge the flower bud stage to be called again the stage of sprouting; Within first 7 days of mushroom producing culture process, in order to urge the flower bud stage, the indoor temperature of urging the flower bud stage is 14 ℃～16 ℃, and indoor humidity remains on more than 95%, and indoor carbon dioxide concentration is controlled at below 0.1%, and suitably ventilate every day.
2. the method with the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush according to claim 1, is characterized in that: the moisture of the composts or fertilisers of cultivating obtaining in step b spice is controlled between 62% ~ 64%.
3. the method with the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush according to claim 1, is characterized in that: in step b spice, the time of described dry stirring is 20 minutes, and the time of described wet stirring is 20 minutes.
4. the method with the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush according to claim 1, is characterized in that: in step f cultural hypha, the temperature of described culturing room is controlled at 18 ℃ of left and right, and air humidity is controlled at below 75%, and lucifuge is cultivated.
5. the method with the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush according to claim 1, is characterized in that: in the management of step h mushroom producing culture, the described inhibition stage is: in the time that mushroom bud grows to 1cm～2cm, mushroom producing culture enters into the inhibition stage; The indoor temperature in this stage is controlled at 4 ℃～6 ℃, and humidity remains on more than 85%, and gas concentration lwevel is controlled at below 0.1%, and ventilate to every day, and carries out illumination 1 h ~ 2h of 200LX every day.
6. the method with the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush according to claim 5, is characterized in that: in the management of step h mushroom producing culture, the described encapsulate sheet stage is: in the time that mushroom body grows to high bottle outlet 2cm left and right, enter into the encapsulate sheet stage; This stage need be lived bottleneck with film circle, so that cultivation bottleneck top forms a small-sized gas concentration lwevel district, to impel the mushroom handle growth of Asparagus, suppresses the growth of mushroom cap, and Asparagus is grown by fan shape.
7. the method with the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush according to claim 6, is characterized in that: in the management of step h mushroom producing culture, described indoor temperature of all educating the stage should be controlled at 6 ℃～8 ℃, and air humidity remains on more than 75%; Light, take dark as main, carries out the illumination of 300LX for every 3 days ~ 5 days, and each light application time is 30 minutes ~ 45 minutes.
8. the method with the fragrant woodflour edible fungus culture medium cultivating flammulina velutipes of tabernaemontanus bulrush according to claim 7, it is characterized in that: in step h mushroom producing culture management, described harvest stages is: grow to and enter harvest stages while exceeding film 1cm～2cm when the fruit body of Asparagus.
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