CN110583361A - Edible fungus cultivation method - Google Patents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/64—Cultivation containers; Lids therefor
- A01G18/68—Cultivation bottles
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Abstract
The invention relates to the technical field of edible fungus cultivation materials, in particular to an edible fungus cultivation method, which comprises the following steps: s1 preparing cultivation materials; s2 mixing the cultivation materials; s3 bottling the cultivation material; s4 inoculation; culturing S5 hypha; s6 mycelium stimulation; s7 culture inhibition; s8 growing; s9, packaging and preserving; the method adopts the measures of high-quality raw materials, the best formula, improvement of the cultivation mode and the like, so that the length of the stem of the planted flammulina velutipes is more than 13cm, the flammulina velutipes is stout and pure white, the quality is good, the planting yield is high, and the yield is increased by 8.5%.
Description
Technical Field
The invention relates to the technical field of edible fungus cultivation materials, in particular to an edible fungus cultivation method.
Background
The edible fungus refers to edible mushroom (large-scale fungus) with large fruiting body, and is commonly called mushroom. More than 350 kinds of edible fungi are known in China, and most of the edible fungi belong to the subphylum basidiomycotina.
The golden mushroom is used as an edible fungus and a medicinal fungus with high economic value, and the economic value of the golden mushroom determines that the golden mushroom has a wide market prospect. The industrialized production management key points of the flammulina velutipes are as follows: the industrial edible mushroom (needle mushroom) production technology generally needs about 53 days for one cultivation period.
Biological characteristics: the famous flammulina velutipes, also called as petiolus subulirus, constructus, hackberry, winter mushroom, hackberry, frozen mushroom, golden mushroom, intelligent mushroom, etc., belongs to the order Agaricales, the family Tricholomataceae, the genus Pleurotus, and is a kind of fungi, algae, lichen. The needle mushroom has high medicinal food therapy effect.
The edible and medicinal performance value of the needle mushrooms is as follows: (1) the contents of lysine and arginine are particularly rich, the shiitake mushroom for preventing hypertension and treating liver and gastrointestinal ulcer contains the flammulina velutipes essence, and the nutrition conditions of the cancer-inhibiting flammulina velutipes are as follows: the flammulina velutipes are saprophytic bacteria, can not perform photosynthesis, and nutrients required by life activities can only be absorbed from saprophytic substrates by virtue of mycelia. Supplementing nutrition, preventing diseases and selecting a probiotic nutrient solution: the mushroom is beneficial to being rich in source and is ecological.
According to the determination, the content of the amino acid in the flammulina velutipes is very rich and higher than that in the common mushrooms, particularly the content of lysine is very high, and the lysine has the function of promoting the intelligence development of children. The dried needle mushroom contains 8.87% of protein, 60.2% of carbohydrate and 7.4% of crude fiber, and can be used for preventing and treating ulcer disease after being eaten frequently. The golden mushroom is not only a delicious food, but also a better health food, and the market of the golden mushroom is increasingly wide at home and abroad.
At present, the method for artificially cultivating the flammulina velutipes is multiple, the cultivated flammulina velutipes have the defects that the length of the stipe is less than 13cm, the flammulina velutipes are thin and white, the flammulina velutipes are easy to be clamped between teeth when being eaten, the yield is low, and the cultivation period is long.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the edible mushroom cultivation method, the length of the stem of the cultivated flammulina velutipes is more than 13cm, the flammulina velutipes is stout and pure white, the cultivation yield is high, the cultivation period is short, and the like.
In order to achieve the purpose, the invention is realized by the following technical scheme:
the edible fungus cultivation method comprises the following components in parts by weight: the cultivation method comprises the following steps:
s1 preparation of cultivation material: weighing the main materials and the auxiliary materials according to the requirements, mixing the main materials to obtain the main materials, mixing the auxiliary materials to obtain the auxiliary materials, and weighing the main materials and the auxiliary materials according to the mass ratio of 9: 1;
s2 mixing of cultivation materials: placing the main material and the auxiliary material in a stirring kettle, fully stirring, adding light sodium hydroxide while stirring to adjust the pH value to 6.03-6.26, adjusting the water content of the cultivation material to 60-65%, and cooling at normal temperature to obtain the cultivation material;
s3 bottling of cultivation materials: filling the cultivation material into cultivation bottles according to 850g per bottle, then sterilizing the cultivation material in the cultivation bottles, compacting the surfaces of the cultivation material, namely punching a hole in the middle of the cultivation material in the cultivation bottles, and covering a filter cover to ensure a nutrient source and air for normal growth of hyphae;
s4 inoculation: inoculating the cultured strain into a culture bottle in an aseptic state by adopting a mechanical automatic inoculation mode, wherein the inoculation amount is 30-35 ML/bottle;
s5 hypha culture: culturing mycelium at 15-17 deg.C under 83-86% relative humidity for 21-23d, and culturing in dark room;
s6 mycelium stimulation: after the hyphae grow fully, removing the bottle cap, and scratching old fungus blocks at the bottle mouth off by using a fungus scratching machine to perform fungus scratching operation so as to realize bud promotion;
s7 culture inhibition: after the bud induction is finished, sending the seeds to a bacterium inhibiting room for inhibiting culture, ventilating for 15 mm every 4 hours, and avoiding strong light irradiation during ventilation;
s8 growth: when the stipe is 0.5-1.0cm long and the mushroom body is 2-3cm long, paper tubes are sleeved to harvest, meanwhile, 15W bulbs are hung every 3-5cm above a shelf to generate vertical light to promote the stipe to extend, the growth is carried out after the temperature is controlled at 8-15 ℃, the air relative humidity is 85-90%, and the stipe can be harvested after the growth is carried out for 14-16 days;
s9 packaging and preserving: the picked mushroom bodies are separately placed according to different grade requirements, the connecting parts of the stipe base parts and the culture medium are removed, packaging with different sizes is carried out according to market requirements, and low-temperature refrigeration is carried out after the packaging is finished.
Preferably, the S1 main material comprises the following components in parts by weight: 72-78 parts of corncob, 17-23 parts of wheat bran, 3.2-3.8 parts of corn flour, 1.85-2.15 parts of gypsum powder, 1.42-1.62 parts of soybean flour, 0.95-1.05 parts of calcium superphosphate and 0.93-1.08 parts of white sugar.
Preferably, the S1 auxiliary material is prepared by mixing corn flour, oyster powder and calcium carbonate.
Preferably, the temperature in the bacteriostatic S7 room is 3-5 ℃, the relative humidity is 80-85%, and the culture time is 5-7 d.
Preferably, CO in the cultivation room in S82The content is controlled to be 0.10-0.15%.
Preferably, the diameter of the pileus is 1-2cm and the length of the stipe is 13-15cm when the S8 is harvested.
Preferably, the relative humidity of the cultivation room is kept between 70 and 80 percent 2 to 3 days before the S8 harvest.
Has the advantages that:
the method adopts high-quality raw materials, an optimal formula, improvement of a cultivation mode and other measures, so that the length of the stem of the planted flammulina velutipes is more than 13cm, the flammulina velutipes is stout and pure white, the quality is good, the planting yield is high, and the yield is increased by 8.5%; the planting period is shortened by 2 days, and the economic benefit is greatly improved; the method adopts the bottle for cultivation, can save the using amount of the cultivation medium, fully utilizes the nutrients, and simultaneously ensures the quality consistency and good uniformity of the quality of the needle mushrooms produced in an industrialized and large-scale manner.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
the edible fungus cultivation method comprises the following components in parts by weight: the cultivation method comprises the following steps:
s1 preparation of cultivation material: weighing raw materials of a main material and an auxiliary material according to requirements, mixing the main raw materials to obtain the main material, mixing the raw materials of the auxiliary material to obtain the auxiliary material, weighing the main material and the auxiliary material according to a mass ratio of 9:1, and mixing the auxiliary material with corn flour, oyster powder and calcium carbonate;
s2 mixing of cultivation materials: placing the main material and the auxiliary material in a stirring kettle, fully stirring, adding light sodium hydroxide while stirring to adjust the pH value to 6.03, adjusting the water content of the cultivation material to 65%, and cooling at normal temperature to obtain the cultivation material;
s3 bottling of cultivation materials: filling the cultivation material into cultivation bottles according to 850g per bottle, then sterilizing the cultivation material in the cultivation bottles, compacting the surfaces of the cultivation material, namely punching a hole in the middle of the cultivation material in the cultivation bottles, and covering a filter cover to ensure a nutrient source and air for normal growth of hyphae;
s4 inoculation: inoculating the cultured strain into a culture bottle in an aseptic state by adopting a mechanical automatic inoculation mode, wherein the inoculation amount is 35 ML/bottle;
s5 hypha culture: culturing mycelium at 15 deg.C under 83% relative humidity for 21d, and culturing in dark room;
s6 mycelium stimulation: after the hyphae grow fully, removing the bottle cap, and scratching old fungus blocks at the bottle mouth off by using a fungus scratching machine to perform fungus scratching operation so as to realize bud promotion;
s7 culture inhibition: after the bud induction is finished, sending the bud to a bacteriostasis chamber for inhibition culture, wherein the temperature in the bacteriostasis chamber is 3 ℃, the relative humidity is 80%, the culture time is 5d, ventilation is carried out for 15 mm every 4h, and strong light irradiation is avoided during ventilation;
s8 growth: starting to sleeve a paper tube to harvest when the stipe is 0.5cm long and the mushroom body is 2cm long, hanging a 15W bulb above the shelf every 3cm to generate vertical light to promote the stipe to extend, wherein the growth is realized by controlling the temperature at 8 ℃, the relative humidity of air at 90% and CO in a cultivation room2Controlling the content to be 0.15%, harvesting after the mushroom grows for 16 days, wherein the diameter of a pileus is 1.2cm, the length of a stipe is 13cm, and keeping the relative humidity of the cultivation room at 72% 2 days before harvesting;
s9 packaging and preserving: the picked mushroom bodies are separately placed according to different grade requirements, the connecting parts of the stipe base parts and the culture medium are removed, packaging with different sizes is carried out according to market requirements, and low-temperature refrigeration is carried out after the packaging is finished.
The S1 main material comprises the following components in parts by weight: 72 parts of corncob, 23 parts of wheat bran, 3.8 parts of corn flour, 1.85 parts of gypsum powder, 1.42 parts of soybean flour, 0.95 part of calcium superphosphate and 0.93 part of white sugar.
Example 2:
the edible fungus cultivation method comprises the following components in parts by weight: the cultivation method comprises the following steps:
s1 preparation of cultivation material: weighing raw materials of a main material and an auxiliary material according to requirements, mixing the main raw materials to obtain the main material, mixing the raw materials of the auxiliary material to obtain the auxiliary material, weighing the main material and the auxiliary material according to a mass ratio of 9:1, and mixing the auxiliary material with corn flour, oyster powder and calcium carbonate;
s2 mixing of cultivation materials: placing the main material and the auxiliary material in a stirring kettle, fully stirring, adding light sodium hydroxide while stirring to adjust the pH value to 6.09, adjusting the water content of the cultivation material to 60%, and cooling at normal temperature to obtain the cultivation material;
s3 bottling of cultivation materials: filling the cultivation material into cultivation bottles according to 850g per bottle, then sterilizing the cultivation material in the cultivation bottles, compacting the surfaces of the cultivation material, namely punching a hole in the middle of the cultivation material in the cultivation bottles, and covering a filter cover to ensure a nutrient source and air for normal growth of hyphae;
s4 inoculation: inoculating the cultured strain into a culture bottle in an aseptic state by adopting a mechanical automatic inoculation mode, wherein the inoculation amount is 30 ML/bottle;
s5 hypha culture: culturing mycelium at 16 deg.C under 84% relative humidity for 22d, and culturing in dark room;
s6 mycelium stimulation: after the hyphae grow fully, removing the bottle cap, and scratching old fungus blocks at the bottle mouth off by using a fungus scratching machine to perform fungus scratching operation so as to realize bud promotion;
s7 culture inhibition: after the bud induction is finished, sending the bud to a bacteriostasis chamber for inhibition culture, wherein the temperature in the bacteriostasis chamber is 5 ℃, the relative humidity is 82%, the culture time is 7d, the ventilation is 15 mm every 4h, and the strong light irradiation is avoided during ventilation;
s8 growth: when the length of the stipe is 0.7cm and the length of the mushroom body is 2.3cm, paper cylinders are sleeved to harvest, meanwhile, 15W bulbs are hung every 4cm above the storehouse rack, the bulbs generate vertical light,promoting the elongation of stipe, controlling the temperature at 10 deg.C and the relative humidity of air at 85%, and culturing in a room with CO2Controlling the content to be 0.10%, harvesting after 15d of growth, wherein the diameter of a pileus is 1.3cm, the length of a stipe is 14.3cm, and keeping the relative humidity of the cultivation room at 70% 3d before harvesting;
s9 packaging and preserving: the picked mushroom bodies are separately placed according to different grade requirements, the connecting parts of the stipe base parts and the culture medium are removed, packaging with different sizes is carried out according to market requirements, and low-temperature refrigeration is carried out after the packaging is finished.
The S1 main material comprises the following components in parts by weight: 73 parts of corncob, 17 parts of wheat bran, 3.4 parts of corn flour, 2.15 parts of gypsum powder, 1.47 parts of soybean flour, 1.01 parts of calcium superphosphate and 1.08 parts of white sugar.
Example 3:
the edible fungus cultivation method comprises the following components in parts by weight: the cultivation method comprises the following steps:
s1 preparation of cultivation material: weighing raw materials of a main material and an auxiliary material according to requirements, mixing the main raw materials to obtain the main material, mixing the raw materials of the auxiliary material to obtain the auxiliary material, weighing the main material and the auxiliary material according to a mass ratio of 9:1, and mixing the auxiliary material with corn flour, oyster powder and calcium carbonate;
s2 mixing of cultivation materials: placing the main material and the auxiliary material in a stirring kettle, fully stirring, adding light sodium hydroxide while stirring to adjust the pH value to 6.15, adjusting the water content of the cultivation material to 61%, and cooling at normal temperature to obtain the cultivation material;
s3 bottling of cultivation materials: filling the cultivation material into cultivation bottles according to 850g per bottle, then sterilizing the cultivation material in the cultivation bottles, compacting the surfaces of the cultivation material, namely punching a hole in the middle of the cultivation material in the cultivation bottles, and covering a filter cover to ensure a nutrient source and air for normal growth of hyphae;
s4 inoculation: inoculating the cultured strain into a culture bottle in an aseptic state by adopting a mechanical automatic inoculation mode, wherein the inoculation amount is 31 ML/bottle;
s5 hypha culture: culturing mycelium at 17 deg.C under 85% relative humidity for 23d, and culturing in dark room;
s6 mycelium stimulation: after the hyphae grow fully, removing the bottle cap, and scratching old fungus blocks at the bottle mouth off by using a fungus scratching machine to perform fungus scratching operation so as to realize bud promotion;
s7 culture inhibition: after the bud induction is finished, sending the bud to a bacteriostasis chamber for inhibition culture, wherein the temperature in the bacteriostasis chamber is 4 ℃, the relative humidity is 83 percent, the culture time is 6d, the ventilation is 15 mm every 4 hours, and the strong light irradiation is avoided during ventilation;
s8 growth: starting to sleeve a paper tube to harvest when the stipe is 1cm long and the mushroom body is 2.7cm long, hanging a 15W bulb above the shelf every 5cm to generate vertical light to promote the stipe to extend, wherein the growth is realized by controlling the temperature at 15 ℃, the relative humidity of air at 87%, and CO in a cultivation room2Controlling the content at 0.13%, harvesting after 14 days of growth, wherein the diameter of the pileus is 1cm, the length of the stipe is 14.8cm, and the relative humidity of the cultivation room is kept at 70-80% 2 days before harvesting;
s9 packaging and preserving: the picked mushroom bodies are separately placed according to different grade requirements, the connecting parts of the stipe base parts and the culture medium are removed, packaging with different sizes is carried out according to market requirements, and low-temperature refrigeration is carried out after the packaging is finished.
The S1 main material comprises the following components in parts by weight: 75 parts of corncobs, 20 parts of wheat bran, 3.5 parts of corn flour, 2 parts of gypsum powder, 1.5 parts of soybean flour, 1 part of calcium superphosphate and 1 part of white sugar.
Example 4:
the edible fungus cultivation method comprises the following components in parts by weight: the cultivation method comprises the following steps:
s1 preparation of cultivation material: weighing raw materials of a main material and an auxiliary material according to requirements, mixing the main raw materials to obtain the main material, mixing the raw materials of the auxiliary material to obtain the auxiliary material, weighing the main material and the auxiliary material according to a mass ratio of 9:1, and mixing the auxiliary material with corn flour, oyster powder and calcium carbonate;
s2 mixing of cultivation materials: placing the main material and the auxiliary material in a stirring kettle, fully stirring, adding light sodium hydroxide while stirring to adjust the pH value to 6.26, adjusting the water content of the cultivation material to 63%, and cooling at normal temperature to obtain the cultivation material;
s3 bottling of cultivation materials: filling the cultivation material into cultivation bottles according to 850g per bottle, then sterilizing the cultivation material in the cultivation bottles, compacting the surfaces of the cultivation material, namely punching a hole in the middle of the cultivation material in the cultivation bottles, and covering a filter cover to ensure a nutrient source and air for normal growth of hyphae;
s4 inoculation: inoculating the cultured strain into a culture bottle in an aseptic state by adopting a mechanical automatic inoculation mode, wherein the inoculation amount is 32 ML/bottle;
s5 hypha culture: culturing mycelium at 16 deg.C under 86% relative humidity for 21d, and culturing in dark room;
s6 mycelium stimulation: after the hyphae grow fully, removing the bottle cap, and scratching old fungus blocks at the bottle mouth off by using a fungus scratching machine to perform fungus scratching operation so as to realize bud promotion;
s7 culture inhibition: after the bud induction is finished, sending the bud to a bacteriostasis chamber for inhibition culture, wherein the temperature in the bacteriostasis chamber is 5 ℃, the relative humidity is 85%, the culture time is 5d, ventilation is carried out for 15 mm every 4h, and strong light irradiation is avoided during ventilation;
s8 growth: the method comprises covering paper tube when the length of stipe is 0.9cm and the length of mushroom body is 3cm, hanging a 15W bulb above the shelf every 4cm to generate vertical light to promote stipe elongation, wherein the growth is controlled at 12 deg.C and air relative humidity is 89%, and CO is in cultivation room2Controlling the content to be 0.12%, harvesting after 15d of growth, wherein the diameter of a pileus is 2cm, the length of a stipe is 15cm, and keeping the relative humidity of the cultivation room at 76% 2d before harvesting;
s9 packaging and preserving: the picked mushroom bodies are separately placed according to different grade requirements, the connecting parts of the stipe base parts and the culture medium are removed, packaging with different sizes is carried out according to market requirements, and low-temperature refrigeration is carried out after the packaging is finished.
The S1 main material comprises the following components in parts by weight: 77 parts of corncob, 21 parts of wheat bran, 3.2 parts of corn flour, 1.93 parts of gypsum powder, 1.62 parts of soybean flour, 1.03 parts of calcium superphosphate and 1.03 parts of white sugar.
Example 5:
the edible fungus cultivation method comprises the following components in parts by weight: the cultivation method comprises the following steps:
s1 preparation of cultivation material: weighing raw materials of a main material and an auxiliary material according to requirements, mixing the main raw materials to obtain the main material, mixing the raw materials of the auxiliary material to obtain the auxiliary material, weighing the main material and the auxiliary material according to a mass ratio of 9:1, and mixing the auxiliary material with corn flour, oyster powder and calcium carbonate;
s2 mixing of cultivation materials: placing the main material and the auxiliary material in a stirring kettle, fully stirring, adding light sodium hydroxide while stirring to adjust the pH value to 6.19, adjusting the water content of the cultivation material to 64%, and cooling at normal temperature to obtain the cultivation material;
s3 bottling of cultivation materials: filling the cultivation material into cultivation bottles according to 850g per bottle, then sterilizing the cultivation material in the cultivation bottles, compacting the surfaces of the cultivation material, namely punching a hole in the middle of the cultivation material in the cultivation bottles, and covering a filter cover to ensure a nutrient source and air for normal growth of hyphae;
s4 inoculation: inoculating the cultured strain into a culture bottle in an aseptic state by adopting a mechanical automatic inoculation mode, wherein the inoculation amount is 33 ML/bottle;
s5 hypha culture: culturing mycelium at 15 deg.C under 85% relative humidity for 22d, and culturing in dark room;
s6 mycelium stimulation: after the hyphae grow fully, removing the bottle cap, and scratching old fungus blocks at the bottle mouth off by using a fungus scratching machine to perform fungus scratching operation so as to realize bud promotion;
s7 culture inhibition: after the bud induction is finished, sending the bud to a bacteriostasis chamber for inhibition culture, wherein the temperature in the bacteriostasis chamber is 3 ℃, the relative humidity is 84%, the culture time is 6d, the ventilation is 15 mm every 4h, and the strong light irradiation is avoided during ventilation;
s8 growth: starting to sleeve a paper tube to harvest when the stipe is 0.6cm long and the mushroom body is 2.3cm long, hanging a 15W bulb above the shelf every 3cm to generate vertical light to promote the stipe to extend, wherein the growth is realized by controlling the temperature at 9 ℃ and the relative humidity of air at 86 percent and using CO in a cultivation room2The content is controlled at 0.12%, and the harvesting can be carried out after the growth for 14 daysWhen in harvesting, the diameter of the pileus is 2cm, the length of the stipe is 15cm, and 2 days before harvesting, the relative humidity of the cultivation room is kept at 76%;
s9 packaging and preserving: the picked mushroom bodies are separately placed according to different grade requirements, the connecting parts of the stipe base parts and the culture medium are removed, packaging with different sizes is carried out according to market requirements, and low-temperature refrigeration is carried out after the packaging is finished.
The S1 main material comprises the following components in parts by weight: 78 portions of corncob, 19 portions of wheat bran, 3.7 portions of corn flour, 2.08 portions of gypsum powder, 1.62 portions of soybean flour, 1.02 portions of calcium superphosphate and 0.97 portion of white sugar.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (7)
1. The edible fungus cultivation method is characterized by comprising the following components in parts by weight: the cultivation method comprises the following steps:
s1 preparation of cultivation material: weighing the main materials and the auxiliary materials according to the requirements, mixing the main materials to obtain the main materials, mixing the auxiliary materials to obtain the auxiliary materials, and weighing the main materials and the auxiliary materials according to the mass ratio of 9: 1;
s2 mixing of cultivation materials: placing the main material and the auxiliary material in a stirring kettle, fully stirring, adding light sodium hydroxide while stirring to adjust the pH value to 6.03-6.26, adjusting the water content of the cultivation material to 60-65%, and cooling at normal temperature to obtain the cultivation material;
s3 bottling of cultivation materials: filling the cultivation material into cultivation bottles according to 850g per bottle, then sterilizing the cultivation material in the cultivation bottles, compacting the surfaces of the cultivation material, namely punching a hole in the middle of the cultivation material in the cultivation bottles, and covering a filter cover to ensure a nutrient source and air for normal growth of hyphae;
s4 inoculation: inoculating the cultured strain into a culture bottle in an aseptic state by adopting a mechanical automatic inoculation mode, wherein the inoculation amount is 30-35 ML/bottle;
s5 hypha culture: culturing mycelium at 15-17 deg.C under 83-86% relative humidity for 21-23d, and culturing in dark room;
s6 mycelium stimulation: after the hyphae grow fully, removing the bottle cap, and scratching old fungus blocks at the bottle mouth off by using a fungus scratching machine to perform fungus scratching operation so as to realize bud promotion;
s7 culture inhibition: after the bud induction is finished, sending the seeds to a bacterium inhibiting room for inhibiting culture, ventilating for 15 mm every 4 hours, and avoiding strong light irradiation during ventilation;
s8 growth: when the stipe is 0.5-1.0cm long and the mushroom body is 2-3cm long, paper tubes are sleeved to harvest, meanwhile, 15W bulbs are hung every 3-5cm above a shelf to generate vertical light to promote the stipe to extend, the growth is carried out after the temperature is controlled at 8-15 ℃, the air relative humidity is 85-90%, and the stipe can be harvested after the growth is carried out for 14-16 days;
s9 packaging and preserving: the picked mushroom bodies are separately placed according to different grade requirements, the connecting parts of the stipe base parts and the culture medium are removed, packaging with different sizes is carried out according to market requirements, and low-temperature refrigeration is carried out after the packaging is finished.
2. The edible mushroom cultivation method according to claim 1, wherein the S1 main material comprises the following components in parts by weight: 72-78 parts of corncob, 17-23 parts of wheat bran, 3.2-3.8 parts of corn flour, 1.85-2.15 parts of gypsum powder, 1.42-1.62 parts of soybean flour, 0.95-1.05 parts of calcium superphosphate and 0.93-1.08 parts of white sugar.
3. The method for cultivating edible fungi according to claim 1, wherein the method comprises the following steps: the S1 auxiliary materials are formed by mixing corn flour, oyster powder and calcium carbonate.
4. The method for cultivating edible fungi according to claim 1, wherein the method comprises the following steps: the temperature of the S7 bacteriostatic room is 3-5 ℃, the relative humidity is 80-85%, and the culture time is 5-7 d.
5. The method for cultivating edible fungi according to claim 1, wherein the method comprises the following steps: CO in the cultivation room in the S82The content is controlled to be 0.10-0.15%.
6. The method for cultivating edible fungi according to claim 1, wherein the method comprises the following steps: and when the S8 is harvested, the diameter of the pileus is 1-2cm, and the length of the stipe is 13-15 cm.
7. The method for cultivating edible fungi according to claim 1, wherein the method comprises the following steps: and 2-3d before harvesting of the S8, keeping the relative humidity of the cultivation room at 70-80%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111149616A (en) * | 2019-12-31 | 2020-05-15 | 扬州苏江缘食用菌有限公司 | Method for cultivating edible fungi by using edible fungi cultivation bottle |
| CN111296173A (en) * | 2020-03-27 | 2020-06-19 | 江苏华绿生物科技股份有限公司 | Industrialized cultivation medium for velvet antler mushroom and method for cultivating edible fungi |
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| CN111149616A (en) * | 2019-12-31 | 2020-05-15 | 扬州苏江缘食用菌有限公司 | Method for cultivating edible fungi by using edible fungi cultivation bottle |
| CN111296173A (en) * | 2020-03-27 | 2020-06-19 | 江苏华绿生物科技股份有限公司 | Industrialized cultivation medium for velvet antler mushroom and method for cultivating edible fungi |
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