CN104119126A - Production process for flammulina velutipes medium - Google Patents

Abstract

The invention relates to a production process for a flammulina velutipes medium. The flammulina velutipes medium comprises the following raw materials by weight part: 24-28 of corn; 32-38 of bagasse; 16-28 of rice bran; 26-30 of cottonseed hull; and 1.8-2.2 of lime. The production process for the flammulina velutipes medium with the above composition includes: selecting dry and fresh raw materials without mildew and pest; placing the raw materials in water to reach a water content of 60%-65%, and conducting stirring for 30min to form the flammulina velutipes medium; putting the water treated raw materials into a bottle with a specification of 1400mL; employing high-pressure steam for sterilization, when the temperature rises to a maximum of 122DEG C, performing heat preservation for 70min, and finally keeping the bottled medium in a tightly covered state for 30min; when the pressure drops to an atmospheric pressure and the temperature drops to lower than 95DEG C, taking the bottle out of the pan; and transferring the sterilized cultivation bottle into a clean cooling chamber to cool to 18DEG C-22DEG C, then conducting inoculation in terms of a 35-50mL liquid spawn per bottle of medium. The process provided by the invention adopts the leftovers of agricultural and sideline products as the raw materials, fully makes use of agricultural production waste, the raw materials are cheap, and have abundant sources and simple configuration. And good economic benefits can be achieved.

Description

The production technique of culture medium for golden mushroom

Technical field

The present invention is the production technique of a kind of production technique of culture medium of edible fungus, particularly a kind of culture medium for golden mushroom.

Background technology

Needle mushroom is one of of all times famous edible mushrooms, is also one of natural health care extremely selling well on current market.The protein content of needle mushroom is high, and rich amino acids has anti-cancer function, often eats effects such as having preventing hypertension, treatment hepatopathy and stomach ulcer.Needle mushroom is produced through cultivation in substratum by bacterial classification, and existing culture medium for golden mushroom, has multiple formulations, as:

(1) potato sucrose substratum (PSA)

Potato (peeling) 200-250g

Agar 20g-22g

White sugar 20g-22g

Water 1000ml

(2) potato glucose substratum (PDA)

Potato (peeling) 200-250g

Agar 20g-22g

Glucose 20g-22g

Water 1000ml

(3) potato sucrose enriched medium:

Potato 200-250g

MgSO 44g-46g

White sugar 20g-22g

VITMAIN B1 (B2) 98 μ g-100 μ g

KH2P04 2.4g-2.6g

Agar 20g-22g

Water 1000ml

(4) onion soy sauce substratum

Onion 100g-110g

Agar 15-25g

Soy sauce 30g-50g

Sucrose 30g-50g

Water 1000ml

(5) malt extract yeast nutrient agar (MYA)

Malt extract 7g-8g

Agar powder 15g-18

Beans peptone 1g

Yeast extract 0.5g

Water 1000ml

(6) apricot juice substratum

Dry apricot 40g-50g

Agar 15g-25g

Water 1000ml

(7) maize powder medium:

Semen Maydis powder (get and decoct juice) 40g-45g

Agar 20g-30g

Sucrose 10g-12g

Water 1000ml

(8) malt extract medium

Fructus Hordei Germinatus (getting juice) 50g-55g

Agar 15g-18g

Water 1000ml

The raw material sources of above-mentioned substratum, are not the tankage that come from agricultural byproducts, so cost is high, raw material sources are insufficient, have limited the production of needle mushroom, can not meet the demand of people to needle mushroom.

Summary of the invention

The object of the invention is in order to overcome the insufficient one-tenth of existing culture medium for golden mushroom raw material sources

The defect that this is high, the production technique of inventing a kind of culture medium for golden mushroom taking corn cob, bagasse, rice bran, cotton seed hulls etc. as raw material.

The object of the invention is to realize as follows:

Described culture medium for golden mushroom, proportioning is by weight as follows:

Corn cob 24-28

Bagasse 32-38

Rice bran 16-28

Cotton seed hulls 26-30

Lime 1.8-2.2

The technique of producing culture medium for golden mushroom by above-mentioned starting material is as follows:

One. selecting of culture medium for golden mushroom raw material

Ingredient requirement is dry, fresh, without going mouldy, without insect pest;

The water content of raw material is below 15%;

After raw material pulverizing, its granular size is below 5mm, and grain thickness degree is even;

Two. the preparation of culture medium for golden mushroom

By the proportioning of described weight part, the raw material after pulverizing is put into water, make its water content reach 60%-65%, stir 30 minutes, pH is controlled at 6.0-6.5.

Three. bottling

The raw material of crossing water is entered in bottling, bottle used is PP material, its specification is 1400mL, bottleneck size is 85mm, after charging is good, through charge level compacting, once punching, secondary punching, punching is exactly to prick from top to bottom hole with chopsticks, prick at the bottle end always, be convenient to the bacterial classification access bottle end, twice punching main purpose is for making hole forming good;

Four. sterilizing

By the substratum of bottling, adopt high pressure steam sterilization, first temperature is risen to 105 DEG C, insulation 20min; Then by temperature rise to 110 DEG C, be incubated 60min; Temperature is risen to 122 DEG C again, in the situation that pressure is 0.131MPa, be incubated 70min, finally the vexed 30min that puts again;

Five. cooling

Until pressure is reduced to normal pressure, temperature and is reduced to 95 DEG C and take the dish out of the pot when following.Sterilizing moves to clean cooling room by culturing bottle later, makes temperature be cooled to 18 DEG C-22 DEG C;

Six. inoculation

The inoculation of use automatic vaccination machine, every bottle of substratum connects the liquid spawn of 35-50mL, inoculation

Temperature is controlled at 18 DEG C-22 DEG C.

Positively effect of the present invention is as follows: the raw material that the present invention uses is the tankage of agricultural byproducts, takes full advantage of agriculture production waste material, and cost of material is cheap, and source is abundant, and configuration is simple, good in economic efficiency.

Embodiment

Described culture medium for golden mushroom, its proportioning raw materials is corn cob 25kg bagasse 35kg, rice bran 20kg, cotton seed hulls 28kg, lime 2kg, makes its water content reach 60%-65%, stirs 30 minutes, and pH is controlled at 6.0-6.5.

The technical process of producing culture medium for golden mushroom by above-mentioned starting material is described as follows:

One. needle mushroom raw material is selected

Choose dry, fresh, corn cob without going mouldy, without insect pest, bagasse, rice bran, cotton seed hulls, lime etc., grain thickness degree is even.

Regulation:

1) water content of corn cob, bagasse, rice bran, cotton seed hulls etc. is below 15%, to prevent that excess moisture from easily producing and going mouldy

2) granular size of corn cob, bagasse, rice bran, cotton seed hulls, lime etc. is below 5mm, excessive to prevent particle, and after bottling, the large water retention capacity of material internal pore is poor, and easily sterilizing is not thorough; Particle is too small, the too consolidation of feeding, and air permeability is poor, sends out bacterium slow.

Two. the preparation of culture material

Pour the raw material of select into large-scale stirrer for mixing by proportioning even, nutrition, pH value, water content that makes the culture material preparing etc. reaches the state that is conducive to Growth of Flammulina Velutipes most.

Regulation:

Proportioning raw materials is corn cob 25kg bagasse 35kg, rice bran 20kg, and cotton seed hulls 28kg, lime 2kg, adds water and makes its water content reach 60%-65%, stirs 30 minutes, and pH is controlled at 6.0-6.5.

Three. bottling

Use bottling machine, needle mushroom compost be encased in culture bottle, then punch, gland and transport.

Regulation:

It is consistent that culture bottle weight after bottling is wanted, and tighten lower pine, only in this way just can make air permeability good, send out bacterium homogeneous

Four. sterilizing

Culturing bottle is placed in high-pressure steam sterilizing pan, and the method adding high pressure with high temperature is killed all microorganisms and gemma thereof.

Regulation:

1) to reach 1.5-2h the working lipe of high pressure steam sterilization

2) stroke of high pressure steam sterilization

First temperature is risen to 105 DEG C, insulation 20min; Then by temperature rise to 110 DEG C, be incubated 60min; Temperature is risen to 122 DEG C again, in the situation that pressure is 0.131MPa, be incubated 70min; The last vexed 30min that puts.

Five. cooling

Make sterilizing culture bottle later be placed in the optimal temperature that clean cooling room is cooled to 20 DEG C, to be conducive to inoculation.

Regulation:

1)) Autoclave need be provided with two doors, a Men Kaixiang working spaces, a Men Kaixiang cooling room, and so just can effectively prevent that temperature from reducing time, extraneous air is back in bottle and causes pollution;

2) cooling room must be clean;

3) cooling room cold air must discharge evenly, in case it is inhomogeneous to lower the temperature

4) reduce to and when normal pressure, temperature drop to below 95 DEG C, just can open pot door at pressure.

Six. inoculation

By aseptic technique, needle mushroom bacterial classification is linked in culture material, makes needle mushroom bacterial classification in culture material, start growth.

Regulation:

1) flammulina velutipes liquid strains using is good, and growing way is good and pollution-free, and every bottle of substratum connects the liquid spawn of 35-50mL;

2) it is thoroughly clean that transfer room need use the germ-free air flow of circulation, makes indoor maintenance be close to sterile state;

3) transfer room temperature is controlled at 20 DEG C and is advisable.

Seven, bacteria developing period management

1, initial stage-mid-term.Under normal circumstances, inoculation 10-15 days after mycelia can stretch into substratum 20-25mm.Part that mycelia spreads becomes white, centered by can seeing inoculating cave at the bottom of bottle, grows.

2, mid-term-later stage.Send out in the smooth situation of bacterium, inoculate latter about 30 days full bottles and all become white, mycelia has covered whole charge level.

Eight, fertility chamber management.

1, urge flower bud operation.Vegetative hyphae is subject to the mechanical stimulus of mycelium stimulation and low temperature stimulation will form former base, and what mycelium stimulation finished bottle should to be moved to low temperature urges flower bud chamber, within a 2-3 days, keeps humidity, and injured mycelia is recovered.

2, all educate and suppress.All educate for utilizing 8 DEG C of low temperature laxative growths, make former base all educate growth.All the atmospheric moisture 85-90% of brood chamber, makes every effort to maintain the air ambient that approaches state of nature, is generally 2-3 days.Inhibition is to utilize 3-5 DEG C of low temperature to suppress the growth of first extending mushroom body, and the growth of growth mushroom body after promoting, so that all stem length neat and consistent.Suppress chambers temp 3-5 DEG C, humidity 85-90%, below gas concentration lwevel 1000ppm, approximately 7 days inhibition time.

3, fruiting period management.Mushroom body grows 0.5-1cm from bottleneck, moves on to and grows chamber, and room temperature keeps 5-8 DEG C, now carries out illumination, has the effect of volume increase and raising quality.

Claims (1)

1. a production technique for culture medium for golden mushroom, is characterized in that:

Described culture medium for golden mushroom, proportioning is by weight as follows:

Corn cob 24-28

Bagasse 32-38

Rice bran 16-28

Cotton seed hulls 26-30

Lime 1.8-2.2

The technique of producing culture medium for golden mushroom by above-mentioned starting material is as follows:

One. selecting of culture medium for golden mushroom raw material

Ingredient requirement is dry, fresh, without going mouldy, without insect pest;

The water content of raw material is below 15%;

After raw material pulverizing, its granular size is below 5mm, and grain thickness degree is even;

Two. the preparation of culture medium for golden mushroom

By the proportioning of described weight part, the raw material after pulverizing is put into water, make its water content reach 60%-65%, stir 30 minutes, pH is controlled at 6.0-6.5

three. bottling

The raw material of crossing water is entered in bottling, and bottle used is PP material, and its specification is 1400mL, bottleneck size is 85mm, after charging is good, through charge level compacting, once punching, secondary punching, punching is pricked hole from top to bottom with chopsticks exactly, makes, in Air infitration hole, then to build bottle cap;

Four. sterilizing

By the substratum of bottling, adopt high pressure steam sterilization, first temperature is risen to 105 DEG C, insulation 20min; Then by temperature rise to 110 DEG C, be incubated 60min; Temperature is risen to 122 DEG C again, in the situation that pressure is 0.131MPa, be incubated 70min, finally the vexed 30min that puts again;

Five. cooling

Until pressure is reduced to normal pressure, temperature and is reduced to 95 DEG C and take the dish out of the pot when following;

Sterilizing moves to clean cooling room by culturing bottle later, makes temperature be cooled to 18 DEG C-22 DEG C;

Six. inoculation

The inoculation of use automatic vaccination machine, every bottle of substratum connects the liquid spawn of 35-50mL, inoculation

Temperature is controlled at 18 DEG C-22 DEG C.