CN114158424A - Needle mushroom bottling and punching method - Google Patents

Needle mushroom bottling and punching method Download PDF

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Publication number
CN114158424A
CN114158424A CN202010955101.XA CN202010955101A CN114158424A CN 114158424 A CN114158424 A CN 114158424A CN 202010955101 A CN202010955101 A CN 202010955101A CN 114158424 A CN114158424 A CN 114158424A
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China
Prior art keywords
punching
culture medium
hole
particle size
stirring
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CN202010955101.XA
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Chinese (zh)
Inventor
华烨
于自建
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Shandong Hengxin Biology Technology Co ltd
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Shandong Hengxin Biology Technology Co ltd
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Priority to CN202010955101.XA priority Critical patent/CN114158424A/en
Publication of CN114158424A publication Critical patent/CN114158424A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/50Inoculation of spawn
    • A01G18/55Forming inoculation holes

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  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The invention discloses a needle mushroom bottling and punching method, which comprises the following steps: (1) the raw materials of the culture medium are subjected to primary processing, the corncobs are required to be processed into particles with the particle size of less than 6mm, the impurity content of rice bran is less than or equal to 3 percent, and the particle size of bran with the particle size of 1-4mm is more than or equal to 35 percent. (2) Pouring the raw materials processed according to the step into a stirring pot according to a proportion, stirring for 20-30 minutes, adding water until the water content of the culture medium is 65% -70%, and stirring for 50-70 minutes. (3) And (3) filling the culture medium with the well-stirred steps into a cultivation bottle. (4) The first punching is carried out by utilizing a punching tool, the center of the culture medium is punched, the diameter of the hole is 22mm, and the pressing height is 1.7-1.9 cm. (5) And (4) punching for the second time, and uniformly punching six small holes around the punched hole in the step (4), wherein the diameter of each small hole is 14mm, the pressing height is 2.0-2.2cm, and the material level of the culture medium is required to be loose. This openly punches many times, and the level of punching is different, and the hole of the different degree of depth is in the fruiting, and the space is comparatively dispersed, and the environment of fruiting is better.

Description

Needle mushroom bottling and punching method
Technical Field
The present disclosure relates to a needle mushroom bottling and punching method.
Background
The statements in this section merely provide background information related to the present disclosure and may not necessarily constitute prior art.
In the process of planting the flammulina velutipes, the flammulina velutipes compost is required to be filled into a culture bottle, then the culture medium filled into the culture bottle is perforated, and the perforation is favorable for hypha germination in the flammulina velutipes production. However, in the operation link of punching, different punching numbers can cause different growth vigors of needle mushrooms, and different punching depths with different strength can cause different growth vigors of the needle mushrooms, so that the growth of the needle mushrooms can be influenced by the number of the holes, the sizes of the holes and the punching depths when the needle mushrooms are punched in a culture medium.
Disclosure of Invention
The present disclosure provides a needle mushroom bottling and punching method to solve the above problems.
The invention aims to provide a needle mushroom bottling and punching method, which is used for punching a culture medium in a needle mushroom bottle by a standard method so as to improve the fruiting quantity and the unit production average value of needle mushrooms.
In order to achieve the purpose, the following technical scheme is adopted in the disclosure:
a needle mushroom bottling and punching method comprises the following steps:
(1) performing primary processing on a culture medium raw material, wherein corncobs are required to be processed into particles with the particle size of less than 6mm, the impurity content of rice bran is less than or equal to 3 percent, and the particle size of bran with the particle size of 1-4mm is more than or equal to 35 percent;
(2) pouring the raw materials processed according to the step into a stirring pot according to a proportion, stirring for 20-30 minutes, adding water until the water content of the culture medium is 65% -70%, and stirring for 50-70 minutes;
(3) filling the culture medium which is well stirred in the step II into a cultivation bottle;
(4) punching for the first time by using a punching tool, punching at the center of the culture medium, wherein the diameter of the hole is 22mm, and the pressing height is 1.7-1.9 cm;
(5) punching for the second time, and uniformly punching six small holes around the punched hole in the step (4), wherein the diameter of each small hole is 14mm, the pressing height is 2.0-2.2cm, and the material level of the culture medium is required to be loose;
preferably, in the step (4), after the center of the culture medium is perforated, a small hole is punched in the center of the hole, the diameter of the small hole is 20mm, and the depth of the small hole reaches the bottom of the culture bottle.
Preferably, the culture medium is inoculated, and mycelium stimulation is cultured for 21 days after inoculation, and the fruiting stage is carried out.
Compared with the prior art, the beneficial effect of this disclosure is:
1. this openly punch many times, the level of punching is different, and the hole of the different degree of depth is in the mushroom, and the space is comparatively dispersed, and the environment of mushroom is better for cultivation bottle spawn running speed shortens charge level front cover time, reduces the pollution rate, reduces cultivation bottle disability rate. The inoculation efficiency is improved, the inoculation effect is enhanced, and the utilization rate of the bacterial liquid is effectively improved.
The specific implementation mode is as follows:
it should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
In the present disclosure, the relation words determined only for the convenience of describing the structural relationship of the components or elements of the present disclosure, do not refer to any components or elements of the present disclosure, and are not to be construed as limiting the present disclosure.
In the present disclosure, terms such as "fixedly connected", "connected", and the like are to be understood in a broad sense, and mean either a fixed connection or an integrally connected or detachable connection; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present disclosure can be determined on a case-by-case basis by persons skilled in the relevant art or technicians, and are not to be construed as limitations of the present disclosure.
Example 1
A needle mushroom bottling and punching method comprises the following steps:
(1) the raw materials of the culture medium are subjected to primary processing, the corncobs are required to be processed into particles with the particle size of less than 6mm, the impurity content of rice bran is less than or equal to 3 percent, and the particle size of bran with the particle size of 1-4mm is more than or equal to 35 percent.
(2) Pouring the raw materials processed according to the step into a stirring pot according to a proportion, stirring for 20-30 minutes, adding water until the water content of the culture medium is 65% -70%, and stirring for 50-70 minutes.
(3) And (3) filling the culture medium with the well-stirred steps into a cultivation bottle.
(4) Punching for the first time by using a punching tool, punching at the center of the culture medium, wherein the diameter of the hole is 22mm, the pressing height is 1.7-1.9cm, punching at the center of the culture medium, punching a small hole at the center of the hole, the diameter of the small hole is 20mm, and the depth of the small hole reaches the bottom of the culture bottle.
(5) And (4) punching for the second time, and uniformly punching six holes around the punched hole in the step (4), wherein the diameter of the punched hole for the second time is 14mm, the pressing height is 2.0-2.2cm, and the material level of the culture medium is required to be loose.
Then, the mixture was inoculated for 20 days for mycelium stimulation and harvested for 26 days.
Example 2
Steps (1) to (5) As in example 1, after inoculation, the culture was carried out for 21 days, and the fungi were collected for 26 days.
Example 3
Steps (1) to (5) As in example 1, after inoculation, the culture was carried out for 22 days, and the fungi were collected for 26 days.
Comparative data 1
Figure RE-GDA0002910472480000041
Comparative data 2
Figure RE-GDA0002910472480000051
Comparative data 3
Figure RE-GDA0002910472480000052
Figure RE-GDA0002910472480000061
And (4) conclusion: as can be seen by comparing the experimental data,
the number of the spawn running of the method is greater than that of the conventional spawn running, and the number of the produced needle mushrooms is large.
The method has the advantages that the growth time is the same, the fungus growing period is shorter than that of the conventional fungus growing period, and the factory building and the operation cost are saved.
The method has the advantages that the grade rate is higher than that of the conventional method, so that the growth quality of the flammulina velutipes is good, the quality is good, the yield per unit is high, the overall quality of the flammulina velutipes is improved, and the benefit is improved.
The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Although the present disclosure has been described with reference to specific embodiments, it should be understood that the scope of the present disclosure is not limited thereto, and those skilled in the art will appreciate that various modifications and variations can be made without departing from the spirit and scope of the present disclosure.

Claims (3)

1. A needle mushroom bottling and punching method is characterized by comprising the following steps:
(1) performing primary processing on a culture medium raw material, wherein corncobs are required to be processed into particles with the particle size of less than 6mm, the impurity content of rice bran is less than or equal to 3 percent, and the particle size of bran with the particle size of 1-4mm is more than or equal to 35 percent;
(2) pouring the raw materials processed according to the step into a stirring pot according to a proportion, stirring for 20-30 minutes, adding water until the water content of the culture medium is 65% -70%, and stirring for 50-70 minutes;
(3) filling the culture medium which is well stirred in the step II into a cultivation bottle;
(4) punching for the first time by using a punching tool, punching at the center of the culture medium, wherein the diameter of the hole is 22mm, and the pressing height is 1.7-1.9 cm;
(5) and (4) punching for the second time, and uniformly punching six small holes around the punched hole in the step (4), wherein the diameter of each small hole is 14mm, the pressing height is 2.0-2.2cm, and the material level of the culture medium is required to be loose.
2. The method for perforating the bottled needle mushroom according to claim 1, wherein in the step (4), after the hole is drilled in the center of the culture medium, a small hole is drilled in the center of the hole, the diameter of the small hole is 20mm, and the depth of the small hole reaches the bottom of the cultivation bottle.
3. The method for perforating the bottled needle mushroom according to claim 1, wherein the inoculation is performed on a culture medium, and mycelium stimulation is performed for 21 days after the inoculation, and the fruiting stage is performed.
CN202010955101.XA 2020-09-11 2020-09-11 Needle mushroom bottling and punching method Pending CN114158424A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114667891A (en) * 2022-03-16 2022-06-28 重庆华绿生物有限公司 Novel edible mushroom cultivation bottle and use method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101514130A (en) * 2009-03-27 2009-08-26 广东星河生物科技股份有限公司 Formula of high-yield flammulina velutipes culture medium and producing technique thereof
CN102037858A (en) * 2010-09-29 2011-05-04 江苏江南生物科技有限公司 Method for industrially culturing needle mushroom by utilizing soybean stalks
CN104119126A (en) * 2013-04-26 2014-10-29 如意情集团股份有限公司 Production process for flammulina velutipes medium

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101514130A (en) * 2009-03-27 2009-08-26 广东星河生物科技股份有限公司 Formula of high-yield flammulina velutipes culture medium and producing technique thereof
CN102037858A (en) * 2010-09-29 2011-05-04 江苏江南生物科技有限公司 Method for industrially culturing needle mushroom by utilizing soybean stalks
CN104119126A (en) * 2013-04-26 2014-10-29 如意情集团股份有限公司 Production process for flammulina velutipes medium

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114667891A (en) * 2022-03-16 2022-06-28 重庆华绿生物有限公司 Novel edible mushroom cultivation bottle and use method thereof

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