CN101514130A - Formula of high-yield flammulina velutipes culture medium and producing technique thereof - Google Patents
Formula of high-yield flammulina velutipes culture medium and producing technique thereof Download PDFInfo
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- CN101514130A CN101514130A CNA2009101063331A CN200910106333A CN101514130A CN 101514130 A CN101514130 A CN 101514130A CN A2009101063331 A CNA2009101063331 A CN A2009101063331A CN 200910106333 A CN200910106333 A CN 200910106333A CN 101514130 A CN101514130 A CN 101514130A
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- 240000006499 Flammulina velutipes Species 0.000 title claims abstract description 13
- 235000016640 Flammulina velutipes Nutrition 0.000 title claims abstract description 13
- 239000001963 growth medium Substances 0.000 title claims abstract description 11
- 238000000034 method Methods 0.000 title claims abstract description 11
- 238000004519 manufacturing process Methods 0.000 claims abstract description 10
- 240000008042 Zea mays Species 0.000 claims abstract description 8
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 8
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 8
- 235000005822 corn Nutrition 0.000 claims abstract description 8
- 244000068988 Glycine max Species 0.000 claims abstract description 7
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 7
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 7
- 235000009566 rice Nutrition 0.000 claims abstract description 7
- 238000007580 dry-mixing Methods 0.000 claims abstract description 6
- 238000002156 mixing Methods 0.000 claims abstract description 6
- 240000007594 Oryza sativa Species 0.000 claims abstract 2
- 238000003756 stirring Methods 0.000 claims description 18
- 238000011081 inoculation Methods 0.000 claims description 8
- 235000016068 Berberis vulgaris Nutrition 0.000 claims description 6
- 241000335053 Beta vulgaris Species 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- 235000015099 wheat brans Nutrition 0.000 claims description 6
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims description 5
- 235000011613 Pinus brutia Nutrition 0.000 claims description 5
- 241000018646 Pinus brutia Species 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 210000000582 semen Anatomy 0.000 claims description 5
- 230000000638 stimulation Effects 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 4
- 235000013599 spices Nutrition 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 abstract description 11
- 239000002994 raw material Substances 0.000 abstract description 5
- 230000009466 transformation Effects 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 241000609240 Ambelania acida Species 0.000 abstract 1
- 241000233866 Fungi Species 0.000 abstract 1
- 239000010905 bagasse Substances 0.000 abstract 1
- 235000013312 flour Nutrition 0.000 abstract 1
- 208000015181 infectious disease Diseases 0.000 abstract 1
- 239000002075 main ingredient Substances 0.000 abstract 1
- 241000218645 Cedrus Species 0.000 description 7
- 241000209094 Oryza Species 0.000 description 5
- 230000007613 environmental effect Effects 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 238000004080 punching Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000010938 white gold Substances 0.000 description 1
- 229910000832 white gold Inorganic materials 0.000 description 1
Abstract
The present invention provides a formula of high-yield flammulina velutipes culture medium and a producing technique thereof. The invention relates to the technical filed of biological strain, and specially to the formula of high-yield flammulina velutipes culture medium and producing technique thereof. The corncob is used as main ingredient for replacing fir sawdust. The invention adopts 40-45% of corncob, 10-12% of bagasse, 25-30% of rice bran, 8-10% of bran, 4-6% of soybean hull and 2-3% of corn flour as the raw materials of flammulina velutipes culture medium. The method comprises the techniques of dry mixing, wet mixing, etc. The pH value is controlled between 7.0 and 7.5. The cultured flammulina velutipes has the advantages of strong activity of mycelium, high production volume, ordered mushroom generation, excellent commodity valve, ordered growing of sporphore, uniform magnitude of bonnet, white color, and no infection of foreign fungus. The unit production obtains 286-293grams/bottle. The dry object of each bottle is 260grams. The biological transformation efficiency reaches up to 110-113% and is increased for 30-40% compared with the common formula. The economic benefit is excellent.
Description
Technical field:
The present invention relates to the biological inoculum technical field, refer to a kind of formula of high-yield flammulina velutipes culture medium and production technique especially.
Background technology:
Needle mushroom is rich in extremely liking of people of multiple amino acid to the human body beneficial because of it, and nourishing function such as the children ' s intelligence development of promotion is arranged.And the production of needle mushroom all is by inoculating on substratum, and then turns out mycelia, thereby the production technique of results product is carried out.And the general portion of existing culture medium for golden mushroom is made of following composition:
Golden mushroom plantation substratum contrast design (unit: %)
| Prescription | Boll hull | Cedar sawdust | Rice bran | Beet pulp | Soybean hulls | Corn cob | Sucrose | Wheat bran |
| I | 30 | 58 | 10 | \ | \ | \ | 2 | In right amount |
| II | 20 | 64 | 14 | 1 | 5 | 21 | 2 | In right amount |
From above-mentioned tabulation as can be seen, the main composition of its substratum is a cedar sawdust, and cedar sawdust is as a kind of environmental protection resource, a large amount of employings is the trend that does not meet the whole world development of environmental protection at present, and it also has been subjected to the restriction of scarcity of resources as raw materials for production, has also made the cedar sawdust that is provided in the market under-supply present situation occur.
Summary of the invention:
The objective of the invention is to overcome above-mentioned weak point of the prior art, and a kind of formula of high-yield flammulina velutipes culture medium and production technique are provided.
The present invention is achieved through the following technical solutions:
Formula of high-yield flammulina velutipes culture medium is:
High-yield flammulina velutipes culture medium for cultivating contrast design (unit: %)
| Prescription | Boll hull | Cedar sawdust | Rice bran | Beet pulp | Soybean hulls | Corn cob | Semen Maydis powder | Wheat bran |
| III | \ | \ | 25～ 30 | 10～ 12 | 4～6 | 40～ 45 | 2～3 | 8～10 |
The high-yield flammulina velutipes production technique is:
1, spice: pour above-mentioned substratum into stirrer and mix 20～25 minutes (the dry mixing time) of stirring, add water, make the moisture content of substratum reach 60～68%, stir 40～45 minutes (wet-mixing time), make its pH value between 7.0～7.5 while stir;
2, bottling: be delivered into the luggage bottle with travelling belt, bottling requires to tighten down pine, and the important constant weight of charging bottle, to guarantee consistency of product
3, sterilization: advance then and sterilized in the autoclave 2.5 hours, cooling draws stove to be cooled to 18～20 degree (requiring: will be controlled within 2 hours to the propelling autoclave from spice) then;
4, inoculation: in aseptic working space operation, inoculum size is wanted evenly;
5, cultivate: connect the culture bottle of planting and delivered in the cultivation room, the temperature of cultivating the room is set at 16～20 degree, and humidity is 68～70%, covers with through 20～25 days mycelia, can carry out the mycelium stimulation fruiting.
Characteristics of the present invention are: mycelia is energetic, the output height, and fruiting is neat, and the commodity value of needle mushroom is good, sporophore growth is neat, and the cap size is even, and color is great white, do not infect, per unit area yield Chuang Dar Industrial Co., Ltd 286～293 gram/bottles, every bottle of siccative 260 grams, the biology transformation efficiency is up to 110～113%, improve 30～40% than common prescription, good in economic efficiency; Another outstanding characteristics are: owing to do not add cedar sawdust and cotton seed hull in the prescription, its bacterium chaff is rich in rich in protein and fat, be of value to digestion and the absorption of animal, be used for being processed into animal-feed, feeding stock animals can increase economic benefit again, both has been of value to environmental protection, also reach the biological purpose of utilizing again, met very much the circular economy policy of country.
Embodiment:
Embodiment 1:
Get rice bran 25; Beet pulp 10; Soybean hulls 4; Corn cob 43; Semen Maydis powder～3; Wheat bran 8; (% by volume)
1, the above-mentioned raw materials of getting ready is poured into 20 minutes (the dry mixing time) of stirring in the stirrer;
2, add water while stirring, make the moisture content of substratum reach 60～68%, stir 40 minutes (wet-mixing time), make its pH value between 7.0～7.5, stirring must be even;
3, be delivered into the luggage bottle with travelling belt, bottling requires to tighten down pine, and constant weight is wanted in bottling, every bottled siccative 260g/800ml, and every bottle of centre covers the filtration bottle cap then to the punching of the bottle end, to guarantee the mycelial growth uniformity;
4, advance and to sterilize in the autoclave that (pressure was 1.5kg/cm in 2.5 hours
2, 121 ° of C` of temperature), then the cooling draw stove be cooled to 18～20 the degree (carrying out) in the clean room;
5, inoculation (carry out mechanical inoculation under aseptic condition, inoculum size is the 10g/ bottle);
6, the culture bottle that has connect kind is delivered to and is cultivated in the room, cultivates the room dark of will demanding perfection, and temperature is set at 16～20 degree, and humidity is 68～70%, covers with through 20～25 days mycelia, can carry out the mycelium stimulation fruiting.
Embodiment 2:
Get rice bran 27; Beet pulp 11; Soybean hulls 5; Corn cob 43; Semen Maydis powder～3; Wheat bran 9; (% by volume)
1, the above-mentioned raw materials of getting ready is poured into 20 minutes (the dry mixing time) of stirring in the stirrer;
2, add water while stirring, make the moisture content of substratum reach 60～68%, stir 40 minutes (wet-mixing time), make its pH value between 7.0～7.5, stirring must be even;
3 usefulness travelling belts are delivered into the luggage bottle, and bottling requires to tighten down pine, and constant weight is wanted in bottling, every bottled siccative 260g/800ml, and every bottle of centre covers the filtration bottle cap then to the punching of the bottle end, to guarantee the mycelial growth uniformity;
4, advance and to sterilize in the autoclave that (pressure was 1.5kg/cm in 2.5 hours
2, 121 ° of C` of temperature), then the cooling draw stove be cooled to 18～20 the degree (carrying out) in the clean room;
5, inoculation (carry out mechanical inoculation under aseptic condition, inoculum size is the 10g/ bottle);
6, the culture bottle that has connect kind is delivered to and is cultivated in the room, cultivates the room dark of will demanding perfection, and temperature is set at 16～20 degree, and humidity is 68～70%, covers with through 20～25 days mycelia, can carry out the mycelium stimulation fruiting.
Embodiment 3:
Get rice bran 30; Beet pulp 12; Soybean hulls 6; Corn cob 45; Semen Maydis powder～3; Wheat bran 10; (% by volume)
1, the above-mentioned raw materials of getting ready is poured into 20 minutes (the dry mixing time) of stirring in the stirrer;
2, add water while stirring, make the moisture content of substratum reach 60～68%, stir 40 minutes (wet-mixing time), make its pH value between 7.0～7.5, stirring must be even;
3 usefulness travelling belts are delivered into the luggage bottle, and bottling requires to tighten down pine, and constant weight is wanted in bottling, every bottled siccative 260g/800ml, and every bottle of centre covers the filtration bottle cap then to the punching of the bottle end, to guarantee the mycelial growth uniformity;
4, advance and to sterilize in the autoclave that (pressure was 1.5kg/cm in 2.5 hours
2, 121 ° of C` of temperature), then the cooling draw stove be cooled to 18～20 the degree (carrying out) in the clean room;
5, inoculation (carry out mechanical inoculation under aseptic condition, inoculum size is the 10g/ bottle);
6, the culture bottle that has connect kind is delivered to and is cultivated in the room, cultivates the room dark of will demanding perfection, and temperature is set at 16～20 degree, and humidity is 68～70%, covers with through 20～25 days mycelia, can carry out the mycelium stimulation fruiting.
As can be seen, the present invention is that major ingredient replaces cedar sawdust with the corn cob, and saving cost, realizes high-quality, high yield, raising utilization of agricultural resources utilization according to the biological characteristics of needle mushroom, reaches the purpose of national circular economy policy from each following table.
Needle mushroom is in each prescription mycelial growth situation
| Prescription | Sprout time (h) | The material feeding time (d) | The long speed of mycelia (mm/d) | Mycelia is covered with bottle (d) | Mycelial density |
| I | 28 | 3 | 4.28 | 22 | +++ |
| II | 29 | 3.5 | 4.28 | 22 | ++ |
| III | 28 | 3 | 4.29 | 21 | +++ |
Each prescription is to the nutrition (g/100g dry product) of needle mushroom
| Prescription | Protein | Crude fat | Robust fibre | Carbohydrate | Ash content | Amino acid |
| I | 14.5 | 1.7 | 6.3 | 61.2 | 3.8 | 20.0 |
| II | 14.7 | 1.6 | 6.2 | 60.1 | 3.9 | 19.8 |
| III | 16.2 | 1.8 | 6.5 | 60.2 | 3.5 | 20.3 |
The grade scale of white gold needle mushroom
| Index | The A level | The B level | The C level | Handle |
| Bacteria cover diameter | ≤1.0cm | 1.-1.2cm | ≥2.0 | - |
| Stem length | 13-15cm | 11-12cm | 9-10cm | - |
Each prescription is to influence (the bottle specification: 800ml) of needle mushroom quality, output, transformation efficiency
| Prescription | A level product rate | Whiteness | Cap hardness | Per unit area yield (g/ bottle) | Biology transformation efficiency (%) |
| I | 95％ | Pure white | +++ | 251 | 76％ |
| II | 90％ | Pure white | ++ | 246 | 80.6％ |
| III | 100％ | Pure white | +++ | 286 | 110％ |
Claims (2)
1, a kind of formula of high-yield flammulina velutipes culture medium is characterized in that: rice bran 25～30; Beet pulp 10～12; Soybean hulls 4～6; Corn cob 40～45; Semen Maydis powder 2～3; Wheat bran 8～10; (% by volume).
2, a kind of high-yield flammulina velutipes production technique comprises
1), bottling: be delivered into the luggage bottle with travelling belt, bottling requires to tighten down pine, and the important constant weight of charging bottle, to guarantee consistency of product;
2), sterilization: advance then and sterilized in the autoclave 2.5 hours, cooling draws stove to be cooled to 18～20 degree (requiring: will be controlled within 2 hours to the propelling autoclave from spice) then;
3), inoculation: in aseptic working space operation, inoculum size is wanted evenly;
4), cultivate: connect the culture bottle of planting and delivered in the cultivation room, the temperature of cultivating the room is set at 16～20 degree, and humidity is 68～70%, covers with through 20～25 days mycelia, can carry out the mycelium stimulation fruiting;
It is characterized in that:
1, spice: pour above-mentioned substratum into stirrer and mix 20～25 minutes (the dry mixing time) of stirring, add water, make the moisture content of substratum reach 60～68%, stir 40～45 minutes (wet-mixing time), make its pH value between 7.0～7.5 while stir.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910106333 CN101514130B (en) | 2009-03-27 | 2009-03-27 | Formula of high-yield flammulina velutipes culture medium and producing technique thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910106333 CN101514130B (en) | 2009-03-27 | 2009-03-27 | Formula of high-yield flammulina velutipes culture medium and producing technique thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101514130A true CN101514130A (en) | 2009-08-26 |
| CN101514130B CN101514130B (en) | 2013-01-09 |
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| CN102153410A (en) * | 2010-12-24 | 2011-08-17 | 天水众兴菌业有限责任公司 | Industrial bottle-cultivation white needle mushroom culture medium and preparation method thereof |
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| CN102823424A (en) * | 2012-08-13 | 2012-12-19 | 合肥福泉现代农业科技有限公司 | Regenerative-fruiting cultivation method of needle mushroom |
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| CN106613345A (en) * | 2016-12-08 | 2017-05-10 | 河池市农业科学研究所 | Cultivation method of needle mushroom |
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| CN113875500A (en) * | 2018-07-04 | 2022-01-04 | 黑龙江省农业科学院土壤肥料与环境资源研究所 | Method for preparing edible fungus culture medium and application of edible fungus culture medium in cultivation of edible fungi |
| CN114967779A (en) * | 2022-05-31 | 2022-08-30 | 江苏国耳生物科技有限公司 | Intelligent stirring control system of edible mushroom cultivation material mixer |
| CN114967779B (en) * | 2022-05-31 | 2023-12-15 | 江苏国耳生物科技有限公司 | Intelligent stirring control system of edible fungi cultivation material stirrer |
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