CN101514130B - Formula of high-yield flammulina velutipes culture medium and producing technique thereof - Google Patents
Formula of high-yield flammulina velutipes culture medium and producing technique thereof Download PDFInfo
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- CN101514130B CN101514130B CN 200910106333 CN200910106333A CN101514130B CN 101514130 B CN101514130 B CN 101514130B CN 200910106333 CN200910106333 CN 200910106333 CN 200910106333 A CN200910106333 A CN 200910106333A CN 101514130 B CN101514130 B CN 101514130B
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Abstract
The present invention provides a formula of high-yield flammulina velutipes culture medium and a producing technique thereof. The invention relates to the technical filed of biological strain, and specially to the formula of high-yield flammulina velutipes culture medium and producing technique thereof. The corncob is used as main ingredient for replacing fir sawdust. The invention adopts 40-45% of corncob, 10-12% of bagasse, 25-30% of rice bran, 8-10% of bran, 4-6% of soybean hull and 2-3% of corn flour as the raw materials of flammulina velutipes culture medium. The method comprises the techniques of dry mixing, wet mixing, etc. The pH value is controlled between 7.0 and 7.5. The cultured flammulina velutipes has the advantages of strong activity of mycelium, high production volume, ordered mushroom generation, excellent commodity valve, ordered growing of sporphore, uniform magnitude of bonnet, white color, and no infection of foreign fungus. The unit production obtains 286-293grams/bottle. The dry object of each bottle is 260grams. The biological transformation efficiency reaches up to 110-113% and is increased for 30-40% compared with the common formula. The economic benefit is excellent.
Description
Technical field:
The present invention relates to the biological inoculum technical field, espespecially a kind of formula of high-yield flammulina velutipes culture medium and production technique.
Background technology:
Needle mushroom is rich in extremely liking of people of multiple amino acid to the human body beneficial because of it, and the nourishing function such as the children ' s intelligence development of promotion is arranged.And the production of needle mushroom all is by inoculating at substratum, and then turns out mycelia, thereby the production technique of results product is carried out.And the general section of existing culture medium for golden mushroom is made of following composition:
Golden mushroom plantation substratum contrast design (unit: %)
| Prescription | Boll hull | Cedar sawdust | Rice bran | Beet pulp | Soybean hulls | Corn cob | Sucrose | Wheat bran |
| I | 30 | 58 | 10 | \ | \ | \ | 2 | In right amount |
| II | 20 | 64 | 14 | 1 | 5 | 21 | 2 | In right amount |
From above-mentioned tabulation, can find out, the main composition of its substratum is cedar sawdust, and cedar sawdust is as a kind of environmental protection resource, a large amount of employings is the trend that does not meet the whole world at present environmental protection development, and it also has been subject to the restriction of scarcity of resources as raw materials for production, also so that under-supply present situation has appearred in the cedar sawdust that provides in the market.
Summary of the invention:
The object of the invention is to overcome above-mentioned weak point of the prior art, and a kind of formula of high-yield flammulina velutipes culture medium and production technique are provided.
The present invention is achieved through the following technical solutions:
Formula of high-yield flammulina velutipes culture medium is:
High-yield flammulina velutipes culture medium for cultivating contrast design (unit: %)
| Prescription | Boll hull | Cedar sawdust | Rice bran | Beet pulp | Soybean hulls | Corn cob | Semen Maydis powder | Wheat bran |
| III | \ | \ | 25～ 30 | 10～ 12 | 4～6 | 40～ 45 | 2～3 | 8～10 |
The high-yield flammulina velutipes production technique is:
1, spice: pour above-mentioned substratum into the stirrer mix and blend 20～25 minutes (dry mixing time), add water while stirring, make the moisture content of substratum reach 60～68%, stir 40～45 minutes (wet-mixing time), make its pH value between 7.0～7.5;
2, bottling: be delivered into the luggage bottle with travelling belt, bottling requires to tighten lower pine, and the important constant weight of charging bottle, to guarantee the consistence of product
3, sterilization: then advance and sterilized in the autoclave 2.5 hours, then cooling draws stove to be cooled to 18～20 degree (requiring: from spice to advancing autoclave will be controlled within 2 hours);
4, inoculation: in aseptic working space operation, inoculum size is wanted evenly;
5, cultivate: finished culture bottle is delivered to and is cultivated in the room, and the Temperature Setting of cultivating the room is 16～20 degree, and humidity is 68～70%, covers with through 20～25 days mycelia, can carry out the mycelium stimulation fruiting.
Characteristics of the present invention are: mycelia is energetic, and output is high, and fruiting is neat, and the commodity value of needle mushroom is good, sporophore growth is neat, and the cap size is even, and color is great white, do not infect, per unit area yield Chuang Dar Industrial Co., Ltd 286～293 grams/bottle, every bottle of siccative 260 grams, the biology transformation efficiency is up to 110～113%, improve 30～40% than common prescription, good in economic efficiency; Another outstanding characteristics are: owing to not adding cedar sawdust and cotton seed hull in the prescription, its bacterium chaff is rich in rich in protein and fat, be of value to digestion and the absorption of animal, be used for being processed into animal-feed, feeding stock animals can increase economic benefit again, both has been of value to environmental protection, also reach the purpose of biological recycling, met very much the circular economy policy of country.
Embodiment:
Embodiment 1:
Get rice bran 25; Beet pulp 10; Soybean hulls 4; Corn cob 43; Semen Maydis powder～3; Wheat bran 8; (by volume %)
1, the above-mentioned raw materials of getting ready is poured into 20 minutes (the dry mixing time) of stirring in the stirrer;
2, add water while stirring, make the moisture content of substratum reach 60～68%, stir 40 minutes (wet-mixing time), make its pH value between 7.0～7.5, stirring must be even;
3, be delivered into the luggage bottle with travelling belt, bottling requires to tighten lower pine, and constant weight is wanted in bottling, every bottled siccative 260g/800ml, and then every bottle of centre covers the filtration bottle cap to the punching of the bottle end, to guarantee the mycelial growth uniformity;
4, advance and to sterilize in the autoclave that (pressure was 1.5kg/cm in 2.5 hours
2, 121 ℃ of ` of temperature), then the cooling draw stove be cooled to 18～20 the degree (carrying out in the clean room);
5, inoculation (carry out mechanical inoculation under aseptic condition, inoculum size is the 10g/ bottle);
6, finished culture bottle is delivered to and is cultivated in the room, cultivates the room dark of will demanding perfection, and Temperature Setting is 16～20 degree, and humidity is 68～70%, covers with through 20～25 days mycelia, can carry out the mycelium stimulation fruiting.
Embodiment 2:
Get rice bran 27; Beet pulp 11; Soybean hulls 5; Corn cob 43; Semen Maydis powder～3; Wheat bran 9; (by volume %)
1, the above-mentioned raw materials of getting ready is poured into 20 minutes (the dry mixing time) of stirring in the stirrer;
2, add water while stirring, make the moisture content of substratum reach 60～68%, stir 40 minutes (wet-mixing time), make its pH value between 7.0～7.5, stirring must be even;
3 usefulness travelling belts are delivered into the luggage bottle, and bottling requires to tighten lower pine, and constant weight is wanted in bottling, every bottled siccative 260g/800ml, and then every bottle of centre covers the filtration bottle cap to the punching of the bottle end, to guarantee the mycelial growth uniformity;
4, advance and to sterilize in the autoclave that (pressure was 1.5kg/cm in 2.5 hours
2, 121 ℃ of ` of temperature), then the cooling draw stove be cooled to 18～20 the degree (carrying out in the clean room);
5, inoculation (carry out mechanical inoculation under aseptic condition, inoculum size is the 10g/ bottle);
6, finished culture bottle is delivered to and is cultivated in the room, cultivates the room dark of will demanding perfection, and Temperature Setting is 16～20 degree, and humidity is 68～70%, covers with through 20～25 days mycelia, can carry out the mycelium stimulation fruiting.
Embodiment 3:
Get rice bran 30; Beet pulp 12; Soybean hulls 6; Corn cob 45; Semen Maydis powder～3; Wheat bran 10; (by volume %)
1, the above-mentioned raw materials of getting ready is poured into 20 minutes (the dry mixing time) of stirring in the stirrer;
2, add water while stirring, make the moisture content of substratum reach 60～68%, stir 40 minutes (wet-mixing time), make its pH value between 7.0～7.5, stirring must be even;
3 usefulness travelling belts are delivered into the luggage bottle, and bottling requires to tighten lower pine, and constant weight is wanted in bottling, every bottled siccative 260g/800ml, and then every bottle of centre covers the filtration bottle cap to the punching of the bottle end, to guarantee the mycelial growth uniformity;
4, advance and to sterilize in the autoclave that (pressure was 1.5kg/cm in 2.5 hours
2, 121 ℃ of ` of temperature), then the cooling draw stove be cooled to 18～20 the degree (carrying out in the clean room);
5, inoculation (carry out mechanical inoculation under aseptic condition, inoculum size is the 10g/ bottle);
6, finished culture bottle is delivered to and is cultivated in the room, cultivates the room dark of will demanding perfection, and Temperature Setting is 16～20 degree, and humidity is 68～70%, covers with through 20～25 days mycelia, can carry out the mycelium stimulation fruiting.
Can find out from each following table, the present invention replaces cedar sawdust according to the biological characteristics of needle mushroom take corn cob as major ingredient, and saving cost, realizes high-quality, high yield, raising utilization of agricultural resources utilization, reaches the purpose of national circular economy policy.
Needle mushroom is in each prescription mycelial growth situation
| Prescription | Sprout time (h) | The material feeding time (d) | The long speed of mycelia (mm/d) | Mycelia is covered with bottle (d) | Mycelial density |
| I | 28 | 3 | 4.28 | 22 | +++ |
| II | 29 | 3.5 | 4.28 | 22 | ++ |
| III | 28 | 3 | 4.29 | 21 | +++ |
Each prescription is to the nutrition (g/100g dry product) of needle mushroom
| Prescription | Protein | Crude fat | Robust fibre | Carbohydrate | Ash content | Amino acid |
| I | 14.5 | 1.7 | 6.3 | 61.2 | 3.8 | 20.0 |
| II | 14.7 | 1.6 | 6.2 | 60.1 | 3.9 | 19.8 |
| III | 16.2 | 1.8 | 6.5 | 60.2 | 3.5 | 20.3 |
The grade scale of white gold needle mushroom
| Index | The A level | The B level | The C level | Process |
| Bacteria cover diameter | ≤1.0cm | 1.-1.2cm | ≥2.0 | - |
| Stem length | 13-15cm | 11-12cm | 9-10cm | - |
Each prescription is on impact (the bottle specification: 800ml) of needle mushroom quality, output, transformation efficiency
| Prescription | A level product rate | Whiteness | Cap hardness | Per unit area yield (g/ bottle) | Biology transformation efficiency (%) |
| I | 95％ | Pure white | +++ | 251 | 76％ |
| II | 90％ | Pure white | ++ | 246 | 80.6％ |
| III | 100％ | Pure white | +++ | 286 | 110％ |
Claims (1)
1. a culture medium for golden mushroom is characterized in that: rice bran 25～30; Beet pulp 10～12; Soybean hulls 4～6; Corn cob 40～45; Semen Maydis powder 2～3; Wheat bran 8～10, by volume %.
2. as adopting a kind of culture medium for golden mushroom claimed in claim 1 to produce the technique of needle mushroom, comprise
1), bottling: be delivered into the luggage bottle with travelling belt, bottling requires to tighten lower pine, and the important constant weight of charging bottle, to guarantee the consistence of product;
2), sterilization: then advance and sterilized in the autoclave 2.5 hours, then cooling draws stove to be cooled to 18～20 degree, from spice to advancing autoclave will be controlled within 2 hours;
3), inoculation: in aseptic working space operation, inoculum size is wanted evenly;
4), cultivate: finished culture bottle is delivered to and is cultivated in the room, and the Temperature Setting of cultivating the room is 16～20 degree, and humidity is 68～70%, covers with through 20～25 days mycelia, can carry out the mycelium stimulation fruiting;
It is characterized in that:
1, spice: poured above-mentioned substratum into the stirrer mix and blend 20～25 minutes, add water while stir, make the moisture content of substratum reach 60～68%, stirred 40～45 minutes, make its pH value between 7.0～7.5.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200910106333 CN101514130B (en) | 2009-03-27 | 2009-03-27 | Formula of high-yield flammulina velutipes culture medium and producing technique thereof |
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| CN 200910106333 CN101514130B (en) | 2009-03-27 | 2009-03-27 | Formula of high-yield flammulina velutipes culture medium and producing technique thereof |
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| CN101514130A CN101514130A (en) | 2009-08-26 |
| CN101514130B true CN101514130B (en) | 2013-01-09 |
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Effective date of registration: 20161209 Address after: Clay industrial city 512000 District of Qujiang city in Guangdong province Shaoguan Patentee after: Shaoguan Starway Bio-technology Co., Ltd. Patentee after: Xichong Hope Land Mushroom Technology Co., Ltd. Patentee after: Guangdong Xinghe Biotechnology Co., Ltd. Address before: No. 83 Tangxia Zhen Jiao Ping Road 523000 Guangdong city of Dongguan Province Patentee before: Guangdong Xinghe Biotechnology Co., Ltd. Patentee before: Shaoguan Starway Bio-technology Co., Ltd. |
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