CN106278466B - A kind of agrocybe cultivation matrix and its preparation and cultural method - Google Patents
A kind of agrocybe cultivation matrix and its preparation and cultural method Download PDFInfo
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- 239000011159 matrix material Substances 0.000 title claims abstract description 43
- 241000222532 Agrocybe Species 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 239000000126 substance Substances 0.000 claims abstract description 61
- 229920000742 Cotton Polymers 0.000 claims abstract description 28
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 24
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 22
- 235000005822 corn Nutrition 0.000 claims abstract description 22
- 235000005824 corn Nutrition 0.000 claims abstract description 22
- 235000013312 flour Nutrition 0.000 claims abstract description 22
- 235000000346 sugar Nutrition 0.000 claims abstract description 14
- 240000000218 Cannabis sativa Species 0.000 claims abstract description 13
- 235000009120 camo Nutrition 0.000 claims abstract description 13
- 235000005607 chanvre indien Nutrition 0.000 claims abstract description 13
- 239000011487 hemp Substances 0.000 claims abstract description 13
- 235000012765 hemp Nutrition 0.000 claims abstract description 13
- 235000012766 marijuana Nutrition 0.000 claims abstract description 13
- 240000008564 Boehmeria nivea Species 0.000 claims abstract description 12
- OYPRJOBELJOOCE-UHFFFAOYSA-N calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000011575 calcium Substances 0.000 claims abstract description 11
- 229910052791 calcium Inorganic materials 0.000 claims abstract description 11
- 239000000470 constituent Substances 0.000 claims abstract description 10
- 240000008042 Zea mays Species 0.000 claims abstract description 7
- 241000722948 Apocynum cannabinum Species 0.000 claims abstract 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 30
- 235000019738 Limestone Nutrition 0.000 claims description 21
- 239000006028 limestone Substances 0.000 claims description 21
- 229960003563 Calcium Carbonate Drugs 0.000 claims description 19
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 19
- 230000000638 stimulation Effects 0.000 claims description 18
- CZMRCDWAGMRECN-GDQSFJPYSA-N Sucrose Natural products O([C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O1)[C@@]1(CO)[C@H](O)[C@@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-GDQSFJPYSA-N 0.000 claims description 17
- 239000005720 sucrose Substances 0.000 claims description 17
- 230000001954 sterilising Effects 0.000 claims description 15
- 229960005069 Calcium Drugs 0.000 claims description 10
- 239000000835 fiber Substances 0.000 claims description 7
- 240000003670 Sesamum indicum Species 0.000 claims description 6
- 235000003434 Sesamum indicum Nutrition 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000006227 byproduct Substances 0.000 claims description 3
- 125000000185 sucrose group Chemical group 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 1
- 235000007164 Oryza sativa Nutrition 0.000 claims 1
- 239000010977 jade Substances 0.000 claims 1
- 235000009566 rice Nutrition 0.000 claims 1
- 239000000796 flavoring agent Substances 0.000 abstract description 3
- 235000019634 flavors Nutrition 0.000 abstract description 3
- 206010020880 Hypertrophy Diseases 0.000 abstract description 2
- 241000894006 Bacteria Species 0.000 description 24
- 230000000052 comparative effect Effects 0.000 description 18
- 238000011081 inoculation Methods 0.000 description 16
- CZMRCDWAGMRECN-UGDNZRGBSA-N D-sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 15
- 241000209149 Zea Species 0.000 description 15
- 238000003756 stirring Methods 0.000 description 12
- 230000000694 effects Effects 0.000 description 10
- 241000722949 Apocynum Species 0.000 description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 238000007792 addition Methods 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 5
- 229910052799 carbon Inorganic materials 0.000 description 5
- 229920002678 cellulose Polymers 0.000 description 5
- 239000001913 cellulose Substances 0.000 description 5
- 229920005610 lignin Polymers 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 239000002994 raw material Substances 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 238000003306 harvesting Methods 0.000 description 4
- 229920002488 Hemicellulose Polymers 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 239000002361 compost Substances 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000001963 growth media Substances 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000002609 media Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N β-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 240000004545 Agrocybe aegerita Species 0.000 description 1
- 235000008121 Agrocybe aegerita Nutrition 0.000 description 1
- 241001660920 Agrocybe chaxingu Species 0.000 description 1
- 241000209134 Arundinaria Species 0.000 description 1
- 235000009024 Ceanothus sanguineus Nutrition 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 210000003734 Kidney Anatomy 0.000 description 1
- 240000003553 Leptospermum scoparium Species 0.000 description 1
- 235000015459 Lycium barbarum Nutrition 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 210000003491 Skin Anatomy 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 235000006468 Thea sinensis Nutrition 0.000 description 1
- 229940088594 Vitamin Drugs 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000259 anti-tumor Effects 0.000 description 1
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Inorganic materials [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 1
- ZOMBKNNSYQHRCA-UHFFFAOYSA-J calcium sulfate hemihydrate Chemical compound O.[Ca+2].[Ca+2].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O ZOMBKNNSYQHRCA-UHFFFAOYSA-J 0.000 description 1
- FYHXNYLLNIKZMR-UHFFFAOYSA-N calcium;carbonic acid Chemical compound [Ca].OC(O)=O FYHXNYLLNIKZMR-UHFFFAOYSA-N 0.000 description 1
- -1 carbon-nitrogen Chemical compound 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 238000005202 decontamination Methods 0.000 description 1
- 230000003588 decontaminative Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000011507 gypsum plaster Substances 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000002195 synergetic Effects 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 229930003231 vitamins Natural products 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Abstract
Matrix and its preparation and cultural method are planted the present invention relates to a kind of agrocybe, the matrix includes following siccatives by weight percentage：Ramie accessory substance 15 20%, bluish dogbane accessory substance 15 20%, hemp accessory substance 15 20% and cotton seed hulls 18 33%, in addition also include wheat bran, corn flour, sugar and the inorganic matter containing calcium constituent.Cultivation agrocybe is carried out using the cultivation matrix of the present invention, the agrocybe planted is fleshy hypertrophy, delicious flavour, quality better, fast growth, biological efficiency is high.
Description
Technical field
The invention belongs to fungus growing technique field, it is related to a kind of agrocybe and plants matrix and its preparation and cultural method.
Background technology
Agrocybe also known as Agrocybe aegerita, Agrocybe chaxingu, Agroeybe cylindracea mycelia etc., are a kind of high protein, low-fat collection nutrition, health care
With the Rare edible fungus of dietotherapy.Tea plant mushroom fruit body delicious flavour, gives off a strong fragrance, and stem is tender and crisp, and cap is plump, fresh and tender
It is tasty, excellent taste, with very strong kidney tonifying effect and antitumor activity, be the big situation of selling well bacterium product in international mushroom class trade market ten it
One, it is known as " China god mushroom ", the per kilogram on Japan, Singapore, the U.S., the European Community and China Hong Kong, Taiwan Province supermarket
Dry product value is high 100 dollars.Investigated according to existing market, agrocybe saves the business taken on a certain scale in Jiangxi, Fujian, Zhejiang etc.
Product are produced, but product is still tightly sold, on the high side, and market potential is still very big, with wide DEVELOPMENT PROSPECT.Agrocybe passes through
Factorial praluction, realizes anniversary fresh mushroom listing, can improve biological efficiency and adapt to the requirement of market diversification, promote tea tree
Mushroom is developed to industrialization, scale and intensive direction.
With the development and the aggravation of market competition of mushroom industry, need badly find suit measures to local conditions, low production cost
Culturing raw material substitute.Culturing raw material variation can make agrocybe manufacturing enterprise flexibly select raw material, it is ensured that the stabilization of industry
Development.Agrocybe, which grows, needs the nutriments such as carbon source, nitrogen source, mineral matter, vitamin, and the country uses cottonseed mostly at present
Shell, corncob and weed tree sawdust etc. are as culture matrix raw material, and mouthfeel is general, influences sales volume.
Crudefiber crop is the industrial crops of the great characteristic of China.The utilization to fiber crops is concentrated mainly on stem skin portion at present, for giving birth to
Fiber is produced, the accessory substance for accounting for biological yield 60% or so is taken as bavin to burn or discarded.Crudefiber crop accessory substance (during fiber crops harvest, is removed tough
Other compositions beyond hide fiber, including sesame slices and boon) in contain abundant cellulose, lignin, trace element, amino acid
Deng.If enabling a large amount of cheap crudefiber crop accessory substances to be fully used, higher benefit has been given play to, it is necessary to make great efforts developing crudefiber crop
Byproduct comprehensive utilization approach.
The content of the invention
It is an object of the invention to provide a kind of cultivation matrix of agrocybe, agrocybe production cost, and agrocybe are reduced
Fast growth, biological efficiency are high.
To achieve the above object, the technical scheme is that：A kind of agrocybe cultivation matrix, including by weight percentage
Following siccatives of meter：Ramie accessory substance 15-20%, bluish dogbane accessory substance 15-20%, hemp accessory substance 15-20% and cotton seed hulls 18-
33%, in addition also include wheat bran, corn flour, sugar and the inorganic matter containing calcium constituent.
Further, the mass percent of wheat bran, corn flour, sucrose, calcium carbonate and pulverized limestone is：Wheat bran 12~18%,
Corn flour 2~5%, sugar 0.5~1.5%, the inorganic matter 0.8~1.2% containing calcium constituent.
The accessory substance includes sesame slices and boon, not including bast fiber.
It is preferred that, the inorganic matter containing calcium constituent is calcium carbonate and pulverized limestone, calcium carbonate/pulverized limestone=1:(0.8~
1.2)。
It is preferred that, the sugar is sucrose or glucose.
It is preferred that, it is aqueous in the matrix to account for the 60~70% of matrix gross weight, natural ph.
The preparation method of above-mentioned cultivation matrix, by crudefiber crop accessory substance, cotton seed hulls, wheat bran, corn flour, sugar, the carbon after crushing
Sour calcium and pulverized limestone are mixed with water by the mass ratio, then carry out autoclaving.
The method that agrocybe is cultivated using above-mentioned cultivation matrix, including inoculation, culture, mycelium stimulation and flower bud, and fruiting
Step.
Further, the culture control temperature is at 22 DEG C~26 DEG C, and humid control is 65~70%.
Further, during flower bud, first three days day and night temperature is maintained at 15 DEG C~20 DEG C, and humidity is 90%~95%；
Flower bud humidity is 85%~90% after 3 days；During fruiting, day and night temperature brings up to 20~25 DEG C, and humidity is 90%~95%.
The content of cellulose 35%-44% of boon, content of lignin 18%-23% in crudefiber crop accessory substance, and with quality
Loose, water imbibition is extremely strong, the preferable physical characteristic of not hardened, gas permeability, suitably does the carbon source matrix of edible mushroom；And sesame slices albumen
Matter content height (15%-24%), sesame slices are easily degraded, can as edible mushroom nitrogen source matrix.Ramie accessory substance content of cellulose：
44%, hemicellulose 11.9%, content of lignin 18.1%, protein content 12.09%；Bluish dogbane accessory substance content of cellulose：
42.31%, hemicellulose 22.58%, content of lignin 23.79%, protein content 12.84%；Hemp accessory substance cellulose contains
Amount：35.85%, hemicellulose 22.4%, content of lignin 12.02%, protein content 11.72%.
In cultivation matrix of the present invention, carbon source is provided using cotton seed hulls, crudefiber crop accessory substance as major ingredient, supplemented by wheat bran and corn flour
Material provides the nitrogen source needed for agrocybe growth.
Compared with prior art, the present invention has the advantages that：
(1) cultivation agrocybe is carried out using the cultivation matrix of the present invention, agrocybe can be utilized effectively in crudefiber crop accessory substance
Crude protein, crude fat and sugar, the agrocybe that plants is fleshy hypertrophy, delicious flavour, quality better, and fast growth is biological
Learn efficiency high (compared to only 17-19% is improved as control with cotton seed hulls culture medium).
(2) crudefiber crop accessory substance is agricultural by-products, is renewable resource, abundance, it is easy to collect, with low cost.Section
The resources such as manpower, coal have been saved, environmental pollution is reduced, have met low-carbon environment-friendly theory.
(3) due to fiber crops, Pesticide use amount is few in process of production, and crudefiber crop accessory substance residues of pesticides are relatively low, do not have transgenosis product
Kind, the edible mushroom produced using it as raw material more has safety guarantee.
Embodiment
With reference to embodiment, the invention will be further described.
The preparation of the cultivation matrix of following embodiments 1~4 and comparative example D1~9 and utilize the matrix carry out cultivation agrocybe
Specific method it is as follows：
(1) crushing of crudefiber crop accessory substance：Sesame slices and boon after drying are ground into the particle that particle diameter is 2mm~5mm；
(2) dispensing：Siccative is mixed with water, the water content of cultivation matrix is controlled；
(3) pack sterilizing：The culture matrix of preparation is loaded to 17cm × 33cm bacterium bag using semi-automatic bagging production line
In, carry out autoclaving, 121 DEG C, 2.5h~3h；
(4) it is inoculated with：It is inoculated with after the compost in bacterium bag and bag is down to room temperature, parent species mycelia is gripped using tweezers
Mode, be seeded to the periphery center of compost, according to inoculation before and after bacterium bag weight difference, keep inoculum concentration be 1% or so.
(5) bacterium germination is managed：Bacterium bag after inoculation is put into the culturing room of dark surrounds and cultivated, indoor temperature is controlled 22
DEG C~26 DEG C, frequent ventilation, holding air is fresh, and humid control is 65~70%；
(6) management of producing mushroom：The intelligent mushroom room that the wet control gas of temperature automatically controlled control is moved into after bacterium bag sends out full bacterium is carried out after mycelium stimulation
Timely flower bud.During flower bud, in order to prevent after mycelium stimulation charge level to dry, head 3d day and night temperatures be maintained at 15 DEG C~20 DEG C, humidity
For 90%~95%.Flower bud humidity is 85%~90% after 3d.Then carry out during fruiting, fruiting, day and night temperature is brought up to
20~25 DEG C, humidity is 90%~95%；
(7) harvest.The mushroom that raising chamber has been cultivated moves to harvesting room, wins first batch of fresh mushroom, and fresh mushroom is gone
The dirty, removal of impurity.Secondary bacterium germination, fruiting then are carried out by above-mentioned steps to bacterium bag.The mushroom that raising chamber has been cultivated moves to harvesting
Room, wins second batch of fresh mushroom, and carry out decontamination, the removal of impurity to fresh mushroom.
Following comparative example D1~9 and embodiment 1~2 pass through adjustment by film solid media wheat bran and the consumption of corn flour
Crudefiber crop accessory substance, the content of cotton seed hulls control carbon-nitrogen ratio.The embodiment of the present invention is with the increase of crudefiber crop accessory substance addition, cotton
The consumption of seed shell is reduced.
Sugar used and the inorganic matter containing calcium constituent of the invention are to provide for sugar and calcium constituent needed for agrocybe growth, sugar
For example can be sucrose or glucose, the inorganic matter containing calcium constituent for example can be calcium carbonate and pulverized limestone.
Embodiment 1
According to ramie accessory substance 15%, bluish dogbane accessory substance 15%, hemp accessory substance 15%, cotton seed hulls 33%, wheat bran 16%,
Corn flour 4%, sucrose 1%, pulverized limestone 0.5%, calcium carbonate 0.5% is formulated, and is mixed to join in mixer, and pH value is 6.5, so
After add water to water content and reach 67.5% or so, stir, obtain culture matrix.According to above-mentioned parameter sterilizing, inoculation, support
Bacterium, mycelium stimulation, flower bud, fruiting.
Embodiment 2
According to ramie accessory substance 20%, bluish dogbane accessory substance 20%, hemp accessory substance 20%, cotton seed hulls 18%, wheat bran 16%,
Corn flour 4%, sucrose 1%, pulverized limestone 0.5%, calcium carbonate 0.5% is formulated, and is mixed to join in mixer, and pH value is 6.5, so
After add water to water content and reach 67.5% or so, stir, obtain culture matrix.According to above-mentioned parameter sterilizing, inoculation, support
Bacterium, mycelium stimulation, flower bud, fruiting.
Embodiment 3
According to ramie accessory substance 17.5%, bluish dogbane accessory substance 17.5%, hemp accessory substance 17.4%, cotton seed hulls 29%, wheat
Bran 12%, corn flour 5%, sucrose 0.5%, pulverized limestone 0.5%, calcium carbonate 0.6% is formulated, and is mixed to join in mixer, pH
It is worth for 6.5, then adds water to water content and reach 60% or so, stir, obtains culture matrix.Sterilized according to above-mentioned parameter,
Inoculation, bacteria, mycelium stimulation, flower bud, fruiting.
Embodiment 4
According to ramie accessory substance 18%, bluish dogbane accessory substance 19%, hemp accessory substance 18.4%, cotton seed hulls 22%, wheat bran
18%, corn flour 2%, sucrose 1.5%, pulverized limestone 0.6%, calcium carbonate 0.5% is formulated, and is mixed to join in mixer, pH value
For 6.5, then add water to water content and reach 70% or so, stir, obtain culture matrix.According to above-mentioned parameter sterilizing, connect
Kind, bacteria, mycelium stimulation, flower bud, fruiting.
Comparative example D1
According to ramie accessory substance 50% (mass percent, similarly hereinafter), cotton seed hulls 28%, wheat bran 16%, corn flour 4%, sugarcane
Sugar 1%, pulverized limestone 0.5%, calcium carbonate 0.5% is formulated, and is mixed to join in mixer, and pH value is 6.5, is then added water to aqueous
Amount reaches 67.5% or so, stirs, and obtains culture matrix.According to above-mentioned parameter sterilizing, inoculation, bacteria, mycelium stimulation, flower bud,
Fruiting.
Comparative example D2
According to ramie accessory substance 78%, wheat bran 16%, corn flour 4%, sucrose 1%, pulverized limestone 0.5%, calcium carbonate 0.5%
Formula, is mixed to join in mixer, pH value is 6.5, then adds water to water content and reach 67.5% or so, stir, obtain
Obtain culture matrix.According to above-mentioned parameter sterilizing, inoculation, bacteria, mycelium stimulation, flower bud, fruiting.
Comparative example D3
According to bluish dogbane accessory substance 50%, cotton seed hulls 28%, wheat bran 16%, corn flour 4%, sucrose 1%, pulverized limestone 0.5%,
Calcium carbonate 0.5% is formulated, and is mixed to join in mixer, and pH value is 6.5, is then added water to water content and is reached 67.5% or so,
Stir, obtain culture matrix.According to above-mentioned parameter sterilizing, inoculation, bacteria, mycelium stimulation, flower bud, fruiting.
Comparative example D4
According to bluish dogbane accessory substance 78%, wheat bran 16%, corn flour 4%, sucrose 1%, pulverized limestone 0.5%, calcium carbonate 0.5%
Formula, is mixed to join in mixer, pH value is 6.5, then adds water to water content and reach 67.5% or so, stir, obtain
Obtain culture matrix.According to above-mentioned parameter sterilizing, inoculation, bacteria, mycelium stimulation, flower bud, fruiting.
Comparative example D5
According to hemp accessory substance 30%, cotton seed hulls 48%, wheat bran 16%, corn flour 4%, sucrose 1%, pulverized limestone 0.5%,
Calcium carbonate 0.5% is formulated, and is mixed to join in mixer, and pH value is 6.5, is then added water to water content and is reached 67.5% or so,
Stir, obtain culture matrix.According to above-mentioned parameter sterilizing, inoculation, bacteria, mycelium stimulation, flower bud, fruiting.
Comparative example D6
According to hemp accessory substance 50%, cotton seed hulls 28%, wheat bran 16%, corn flour 4%, sucrose 1%, pulverized limestone 0.5%,
Calcium carbonate 0.5% is formulated, and is mixed to join in mixer, and pH value is 6.5, is then added water to water content and is reached 67.5% or so,
Stir, obtain culture matrix.According to above-mentioned parameter sterilizing, inoculation, bacteria, mycelium stimulation, flower bud, fruiting.
Comparative example D7
According to hemp accessory substance 78%, wheat bran 16%, corn flour 4%, sucrose 1%, pulverized limestone 0.5%, calcium carbonate 0.5%
Formula, is mixed to join in mixer, pH value is 6.5, then adds water to water content and reach 67.5% or so, stir, obtain
Obtain culture matrix.According to above-mentioned parameter sterilizing, inoculation, bacteria, mycelium stimulation, flower bud, fruiting.
Comparative example D8
According to ramie accessory substance 26%, bluish dogbane accessory substance 26%, hemp accessory substance 26%, cotton seed hulls 0%, wheat bran 16%,
Corn flour 4%, sucrose 1%, pulverized limestone 0.5%, calcium carbonate 0.5% is formulated, and is mixed to join in mixer, and pH value is 6.5, so
After add water to water content and reach 67.5% or so, stir, obtain culture matrix.According to above-mentioned parameter sterilizing, inoculation, support
Bacterium, mycelium stimulation, flower bud, fruiting.
Comparative example D9
Control medium：According to cotton seed hulls 78%, wheat bran 16%, corn flour 4%, sucrose 1%, pulverized limestone 0.5%, carbonic acid
Calcium 0.5% is formulated, and is mixed to join in mixer, and pH value is 6.5, is then added water to water content and is reached 67.5% or so, stirring
Uniformly, culture matrix is obtained.According to above-mentioned parameter sterilizing, inoculation, bacteria, mycelium stimulation, flower bud, fruiting.
Implementation result
Above-described embodiment and comparative example are with the scale of each 40 bottles of processing, and formula is shown in Table 1, the result such as table 2 of processing.
Culture medium prescription table (the unit of table 1：Quality %)
The different substrates of table 2 proportioning cultivation agrocybe effect
Note：Same column difference lowercase letter indication difference significantly (P in table<0.05)
The agrocybe mycelium length of the embodiment of the present invention 1 and 2 is 0.293cmd-1~0.296cmd-1, one batch of fresh mushroom weight
For 95.1g~97g, regrowth hair fresh mushroom weight is 49g~50g, and biological efficiency is 103%~105%.
Comparative example D9 contrast discoveries with not adding crudefiber crop accessory substance, the agrocybe mycelium length ratio of the embodiment of the present invention 1 and 2
Comparative example D9 improves 11%~12%, and one batch of fresh mushroom improves 16%~18% than comparative example D9 again, and regrowth hair fresh mushroom compares comparative example again
D9 improves 25~28%, and biological efficiency improves 16.6%~18.6% than comparative example D9.
By D1 and D2, D3 and D4, D6 and D7 contrast are it can be found that the addition of cotton seed hulls is (although accordingly reduce crudefiber crop
Accessory substance) heavy mycelium length, one batch of fresh mushroom, regrowth hair fresh mushroom weight and biological efficiency can be increased substantially.
D1, D3, D6 and D9 are contrasted and found, in the case where being added without crudefiber crop accessory substance, even if cotton seed hulls is significantly increased
Amount, still result in mycelium length, one batch of fresh mushroom weight, regrowth hair fresh mushroom weight and slightly decline with biological efficiency.
Embodiment 2 and D8 are contrasted and found, in the case where the species and amount that crudefiber crop accessory substance is added are suitable, cotton is added without
Seed shell can't cause heavy mycelium length, one batch of fresh mushroom, regrowth hair fresh mushroom weight and biological efficiency significant change.
D8 and D9 contrasts find that, in the case where the total amount of crudefiber crop accessory substance and cotton seed hulls is suitable, cotton seed hulls is to mycelia length
Speed, one batch of fresh mushroom weight and biological efficiency impact effect are more notable.
Contrast D1, D3, D6 to find, the effect of three kinds of crudefiber crop accessory substances is substantially similar, but ramie accessory substance can be carried substantially
High biological efficiency.
It can illustrate herein, the addition control of cotton seed hulls can improve effect within the specific limits, crudefiber crop accessory substance
Effect to a certain extent can part replacement cotton seed hulls.Due to above-mentioned " effect of three kinds of crudefiber crop accessory substances is substantially similar ",
Three kinds of crudefiber crop accessory substances be there is no indication that while the effect added can be more preferable than adding same amount of single crudefiber crop accessory substance.
But, embodiment 3 and D1, D3, D6 are contrasted, in the case where cotton seed hulls addition is essentially identical, three kinds of crudefiber crops
The addition of accessory substance is a kind of than adding, and can significantly improve heavy mycelium length, one batch of fresh mushroom, regrowth hair fresh mushroom weight and biology effect
Rate.
Therefore, certain Synergistic is generated between three kinds of crudefiber crop accessory substances and between cotton seed hulls in the present invention
Effect.To make further contrast, using following cultivation matrixes：25-38 parts of industrial hemp cane bits, 35-53 parts of weed tree sawdusts,
16-20 portions of wheat bran, 0.8-1.2 portions of plaster of paris, 0.8-1.2 portions of sucrose.According to above-mentioned parameter sterilizing, inoculation, bacteria, mycelium stimulation, urge
Flower bud, fruiting.Found by statistical analysis, the formula mycelial growth rate and biological efficiency are below comparative example D5.
Claims (9)
1. a kind of agrocybe cultivation matrix, it is characterised in that including following siccatives by weight percentage：Ramie accessory substance 15-
20%th, bluish dogbane accessory substance 15-20%, hemp accessory substance 15-20% and cotton seed hulls 18-33%, in addition also include wheat bran, jade
Ground rice, sugar and the inorganic matter containing calcium constituent, wheat bran 12~18%, corn flour 2~5%, sugar 0.5~1.5%, the nothing containing calcium constituent
Machine thing 0.8~1.2%.
2. agrocybe cultivation matrix according to claim 1, it is characterised in that the accessory substance includes sesame slices and boon, no
Including bast fiber.
3. the agrocybe cultivation matrix according to one of claim 1~2, it is characterised in that the inorganic matter containing calcium constituent
For calcium carbonate and pulverized limestone, calcium carbonate/pulverized limestone=1:(0.8~1.2).
4. the agrocybe cultivation matrix according to one of claim 1~2, it is characterised in that the sugar is sucrose or grape
Sugar.
5. the agrocybe cultivation matrix according to one of claim 1~2, it is characterised in that aqueous in the matrix to account for matrix total
The 60~70% of weight, natural ph.
6. the preparation method of one of a kind of Claims 1 to 5 cultivation matrix, it is characterised in that by the crudefiber crop by-product after crushing
Thing, cotton seed hulls, wheat bran, corn flour, sugar, calcium carbonate and pulverized limestone are mixed with water by the mass ratio, then enter horizontal high voltage
Sterilizing.
7. the method that a kind of one of utilization Claims 1 to 55 cultivation matrix cultivates agrocybe, it is characterised in that including connecing
Kind, culture, mycelium stimulation and flower bud, and the step of fruiting.
8. the method for cultivation agrocybe according to claim 7, it is characterised in that culture control temperature 22 DEG C~
26 DEG C, humid control is 65~70%.
9. the method for cultivation agrocybe according to claim 7, it is characterised in that during flower bud, first three days is warm round the clock
Difference is maintained at 15 DEG C~20 DEG C, and humidity is 90%~95%；Flower bud humidity is 85%~90% after 3 days；During fruiting, round the clock
The temperature difference brings up to 20~25 DEG C, and humidity is 90%~95%.
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