CN110301286A - A kind of green soilless culture method of ganoderma lucidum - Google Patents

A kind of green soilless culture method of ganoderma lucidum Download PDF

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Publication number
CN110301286A
CN110301286A CN201910631870.1A CN201910631870A CN110301286A CN 110301286 A CN110301286 A CN 110301286A CN 201910631870 A CN201910631870 A CN 201910631870A CN 110301286 A CN110301286 A CN 110301286A
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culture
lucidum
ganoderma
green
ganoderma lucidum
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郑华方
谷浩
谢中品
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Huamao (huzhou) Health Products Co Ltd
Pharmanex Huzhou Health Products Co Ltd
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Huamao (huzhou) Health Products Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/60Cultivation rooms; Equipment therefor
    • A01G18/69Arrangements for managing the environment, e.g. sprinklers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01MCATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00Stationary means for catching or killing insects
    • A01M1/02Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
    • A01M1/04Attracting insects by using illumination or colours

Abstract

A kind of green soilless culture method of ganoderma lucidum, comprising the following steps: (1) tea extract is added in bacterium culture medium, constant temperature is protected from light culture at 24 ~ 28 DEG C by strain;(2) tealeaf residue is crushed, is uniformly mixed with sawdust, wheat bran, obtains culture material;(3) cultivation Bag Material is made in culture material loading strain bag, cooling after the processing of high temperature and pressure moist heat sterilization, access lucidum strain carries out 30 ~ 40d of culture;(4) ganoderma lucidum after the completion of step 3 culture is transferred in greenhouse and carries out out sesame management;(5) mature fructification is harvested, after the completion of three batches of culture harvestings, adds nitrogen source and carbon source nutriment, continue the culture of four batches and five batches, harvest.The present invention program can not only grow high-quality, non-harmful ganoderma lucidum fruitbody, and incubation green, environmental protection, and the ganoderma lucidum of harvest is nuisanceless ganoderma lucidum.

Description

A kind of green soilless culture method of ganoderma lucidum
Technical field
The invention belongs to cultivation of glossy ganoderma technical fields, and in particular to a kind of green soilless culture method of ganoderma lucidum.
Background technique
Ganoderma lucidum (Ganoderma lucidum) is called polyporus lucidus, is the high large-scale load of a kind of nutrition, health value Bacterium.Since ancient times, ganoderma lucidum is regarded as lucky, rich and honour, fine and long-lived symbol, there is the title of " mesona " and " seocho ", in The precious Chinese herbal medicine that magnificent traditional medicine is considered as always nourishing and fit keeping function for a long time, consolidates righting, have invigorating heart gas, tranquilizing the mind tonifying liver, Hard muscles and bones, sharp joint, a variety of functions such as control deafness.Ganoderma lucidum grows by many factors such as nutrition, temperature, moisture, illumination, pH values It influences, substituting stuff cultivation raw material is extensive, technically simple, remarkable in economical benefits.
Existing more than 60 years history of China's ganoderma lucidum artificial cultivation, Wild ganoderma resource is limited, in recent years, the exploitation benefit of ganoderma lucidum With being increasingly valued by people, consumption figure rapid development, therefore the scale of Ganoderma Lucidum is increasing, occurs therewith The diversification of cultivation technique, mode and raw material.Ganoderma lucidum is the wooden saprophytic bacteria, often saprophytic on the dead wood and stub of broad leaf tree, is needed Wanting carbon source, nitrogen source and inorganic salts is nutrient source.But cultivation basswood ganoderma lucidum needs a large amount of log, results in the forest reserves A large amount of consumption, therefore people continually develop the culturing raw material that novel, source is wide, at low cost, efficiently use agriculture and forestry by-product, greatly The cereal crops such as legumes stalk and corncob, corn stalk such as beanstalk, pea straw, semen viciae fabae straw, peanut stems can be employed as ganoderma lucidum The primary raw material of cultivation can not only reduce the production cost of ganoderma lucidum, and can improve the comprehensive utilization ratio of agricultural byproduct.
Substituting stuff cultivation is to make bacterium bag, fabrication processing as culturing raw material using suitable sawdust and farmer's product are as follows: --- --- sterilizing --- goes out sesame and manages --- harvesting --- drying and packaging, such as the application documents of CN102765988A spice for pack Disclose a kind of cultivation of glossy ganoderma Bag Material and the method using the cultivating in bag ganoderma lucidum, the component that Bag Material contains: the beans of 25-35% Straw bits, the bean dregs of 20-30%, the sawdust of 33-53%, 2% land plaster and the yellow plasm of soybean of 1-1.2 times of Bag Material quality of cultivation, solution It has determined the waste utilization problem of agricultural and sideline crop.But the program not can solve in environment in especially soil harmful substance to ganoderma lucidum Pollution, make have the residual such as residual, heavy metal of agriculture in the obtained ganoderma lucidum of cultivation.
Summary of the invention
In view of the above problems, the object of the present invention is to provide a kind of green soilless culture methods of ganoderma lucidum, not only High-quality, non-harmful ganoderma lucidum fruitbody, and incubation green, environmental protection can be grown, the ganoderma lucidum of harvest is nuisanceless spirit Sesame.
To achieve the goals above, technical scheme is as follows:
A kind of green soilless culture method of ganoderma lucidum, comprising the following steps:
Step 1: being added tea extract in bacterium culture medium, and by strain, constant temperature is protected from light culture at 24 ~ 28 DEG C;
Step 2: tealeaf residue is crushed, and is uniformly mixed with sawdust, wheat bran, is obtained culture material;
Step 3: being made cultivation Bag Material for culture material loading strain bag, cooling after the processing of high temperature and pressure moist heat sterilization, access Lucidum strain carries out 30 ~ 40d of culture;
Step 4: the ganoderma lucidum after the completion of step 3 culture is transferred in greenhouse and carries out out sesame management;
Step 5: mature fructification is harvested, and after the completion of three batches of culture harvestings, is added nitrogen source and carbon source nutriment, is continued Carry out four batches and five batches of culture, harvesting.
In this programme design, tea polyphenols, alkaloid and a small amount of amino acid of more content are contained in tea extract Deng tea polyphenols are natural antioxidants, also have bacteriostasis, not only can protect in culture medium the substances such as glucose not It is oxidized, also makes culture medium itself that there is antibacterial, bacteriostasis;Tealeaves alkaloid is the organic compound of a kind of nitrogen-containing heterocycle structure Object can be used as the nutrient source of strain, promote mycelial growth;Amino acid also can be used as the nutrient source of strain, promote mycelium Growth;Tea extract is faintly acid, it is possible to provide suitable weak acid environment is for mycelial growth, therefore, tea extract Conducive to the growth of ganoderma lucidum mycelium, the growth and development of ganoderma lucidum mycelium can be effectively facilitated.The extracted tealeaf residue finished can also be made For culture material, remaining tea polyphenols, alkaloid in tealeaf residue etc. can continue to play the effect for the growth and development that it promotes ganoderma lucidum;Tea Also containing several mineral materials, protein, cellulose, glucose, phosphatide etc. in leaf slag, minerals can effectively improve ganoderma lucidum itself enzyme Activity, can promote the growth and development of ganoderma lucidum, protein, cellulose, glucose etc. can be used as the nutriment of ganoderma lucidum growth Culture material is supplemented, not will cause the waste of resource.
Preferably, the ingredient of culture material includes following parts by weight in the step 2: 60 ~ part of sawdust, 5 ~ 15 parts of wheat bran, tea 5 ~ 20 parts of leaf slag, 1 ~ 3 part of gypsum, 0.5 ~ 1.0 part of sucrose.
Preferably, the preparation step of tea extract includes: that 20 ~ 25 DEG C of water of tealeaves is impregnated 10 in the step 1 It after ~ 14 hours, boils 25 ~ 35 minutes, cooling, gained tealeaf residue is boiled in filtering 1 ~ 2 time again, obtains tealeaves after merging filtered fluid Extracting solution.
Preferably, the granular size of tealeaf residue is 0.2 ~ 2.0cm in the step 2.
Preferably, the sawdust is maple sawdust or chestnut sawdust.
Preferably, in the step 2 processing of culture material include: by wheat bran add water prewet softening after be added sawdust, tealeaves Slag and land plaster stir evenly, and add sucrose, and culture material is sufficiently stirred to obtain.
Preferably, it is described prewet soften the time be 22 ~ 25 hours.
Preferably, the high temperature and pressure moist heat sterilization treatment conditions of Bag Material are cultivated in the step 3 are as follows: 0.14 ~ 0.15MPa, Sterilize at 1.5 ~ 2h or 100 ~ 110 DEG C of sterilizing 10 ~ 12h at 125 ~ 130 DEG C.
Preferably, the condition of sesame management is gone out in the step 4 are as follows: 25 DEG C ~ 30 DEG C of temperature, humidity 80% ~ 90%, titanium dioxide Concentration of carbon scatters illumination 1500-5000Lx less than 0.1%.
Preferably, nitrogen source and carbon source nutriment include glucose, sucrose, protein, crude fibre, half in the step 5 Fiber, lignin etc..
In this programme design, the nutriments such as nitrogen source and carbon source nutriment are used as the supplement to culture material, traditional Cultivation of glossy ganoderma is often only cultivated to three batches, is not just gone on because lacking nutriment, can continue to train after extra-nutrition substance It supports to four batches, five batches, and quality will not be deteriorated, ganoderma lucidum yield can be increased, increase benefit.
Preferably, it is that will cultivate Bag Material to uncap in step 4, is transferred in grid type cultivating stand and further cultivates, cultivates close Degree is 150 ~ 200 Bang/㎡.
In this programme design, soil has been isolated in ganoderma lucidum culture, avoids harmful substance present in soil, miscellaneous bacteria etc. to spirit Sesame pollutes, and is conducive to the temperature of accurate control ganoderma lucidum growth, moisture condition, and the ganoderma lucidum quality made is not in irregular Uneven situation.
The invention has the following advantages:
1. tea extract and tealeaf residue can apply in ganoderma lucidum incubation, the waste of resource, Er Qieneng are not caused not only The growth and development for effectively facilitating ganoderma lucidum, the ganoderma lucidum quality made are higher.
2. the present invention can be cultivated to four batches, five batches compared to three batches of more traditional cultures, economic benefit is improved.
3. water source, temperature, nutriment etc. are precisely controllable in incubation, keep the sub- quality of the ganoderma lucidum of output unified.
4. soil has been isolated in incubation, the pollution of harmful substance in environment is prevented, has made the ganoderma lucidum of harvest without public affairs Evil ganoderma lucidum.
Specific embodiment
Embodiment 1
A kind of green soilless culture method of ganoderma lucidum, comprising the following steps:
Stock: (1) after impregnating fresh tea leaf 12 hours according to the ratio that solid-liquid ratio is 1:10 with 25 DEG C of water, 30 points are boiled Clock is cooled to room temperature, and with filtered through gauze, is boiled again by above-mentioned steps 1 time to gained tealeaf residue, obtains tea after merging filtered fluid twice Leaf extract;(2) tea extract obtained is added into PDA culture medium, ratio 0.05ml/g, is made and is mentioned containing tealeaves Take the PDA culture medium of liquid;(3) remaining tealeaf residue is dried, is crushed between 0.2 ~ 2.0cm;(4) bran for being 15 parts by parts by weight Pi Jiashui prewets after softening, and 60 parts of addition maple tree bits, 10 parts of tealeaf residue and 2 parts of gypsum stir evenly, and adds 0.5 part of sucrose Culture material is sufficiently stirred to obtain, the water content of culture material controls between 60 ~ 65%, and pH value controls between 4 ~ 6.
The production of hybrid seeds: ganoderma lucidum fungus block is placed in the center of the PDA culture medium containing tea extract, 25 DEG C of constant temperature are protected from light culture, right Mycelia growth data is acquired.
Bacterium germination: (1) culture material for preparing is packed into material bag in and cultivating bag is made, through sterilizing at 0.15MPa, 130 DEG C 1.5h accesses strain obtained in the method for sterile working, and strain and culture medium are in close contact, in favor of being colonized and growing;(2) Cultivating bag after inoculation is moved into culturing room's culture, controls temperature between 18 ~ 25 DEG C, between humidity 55 ~ 65%, is protected from light culture 45 days, Ventilation 1 time daily after a week.
Out sesame manage: (1) when in cultivating bag culture medium avoid the occurrence of white or yellow protrusion pimple block, uncap, shift To grid type cultivating stand is pre-set, culture density is 150 Bang/㎡, controls temperature between 25 ~ 30 DEG C, between humidity 80 ~ 90%, And control gas concentration lwevel is below 0.1% and scattering illumination is within the scope of 1500 ~ 5000Lx;(2) every batch of incubation time is 30 It ~ 40 days, is hardened to cap surface presentation paint sample gloss, cap, mature spore constantly comes out below cap, can adopt It receives;(3) after the completion of three batches of harvestings, glucose, protein, lignin are supplemented into cultivating bag, continue culture to four batches, five Stubble.
Insect prevention measure: the greenhouse for cultivating ganoderma lucidum, which is first divulged information, to be exposed to the sun, and quick lime deinsectization, ozonization are spilt in ground, and access door is set Set buffering, ventilation opening is isolated with 40 mesh gauzes around greenhouse, trap lamp is set in canopy, and uniformly install in canopy temperature, humidity, Carbon dioxide probe adjusts temperature, humidity and gas concentration lwevel for monitoring in real time, hard when finding ganoderma lucidum germ and pest Artificial treatment is held, mildew or pollution is found, removes in time, prevent expansion or cross-infection.
Embodiment 2
A kind of green soilless culture method of ganoderma lucidum, comprising the following steps:
Stock: (1) after impregnating fresh tea leaf 10 hours according to the ratio that solid-liquid ratio is 1:8 with 25 DEG C of water, boiling 25 minutes, It is cooled to room temperature, with filtered through gauze, gained tealeaf residue is boiled 2 times again by above-mentioned steps, obtained after merging gained filtered fluid three times Tea extract;(2) tea extract obtained is added into PDA culture medium, ratio 0.02ml/g, is made and contains tealeaves The PDA culture medium of extracting solution;(3) remaining tealeaf residue is dried, is crushed between 0.2 ~ 2.0cm;It (4) is 10 parts by parts by weight Wheat bran adds water to prewet after softening, 80 parts of maple tree bits is added, 5 parts of tealeaf residue and 3 parts of gypsum stir evenly, and adds 1 part of sucrose Culture material is sufficiently stirred to obtain, the water content of culture material controls between 60 ~ 65%, and pH value controls between 4 ~ 6.
The production of hybrid seeds: ganoderma lucidum fungus block is placed in the center of the PDA culture medium containing tea extract, 25 DEG C of constant temperature are protected from light culture, right Mycelia growth data is acquired.
Bacterium germination: (1) culture material for preparing is packed into material bag in and cultivating bag is made, through the 12h that sterilizes at 110 DEG C, with sterile The method of operation accesses strain obtained, and strain and culture medium are in close contact, in favor of being colonized and growing;It (2) will be after inoculation Cultivating bag moves into culturing room's culture, controls temperature between 18 ~ 25 DEG C, between humidity 55 ~ 65%, is protected from light culture 45 days, after a week daily Ventilation 1 time.
Out sesame manage: (1) when in cultivating bag culture medium avoid the occurrence of white or yellow protrusion pimple block, uncap, shift To the grid type cultivating stand pre-set, culture density is 160 Bang/㎡, controls temperature between 25 ~ 30 DEG C, humidity 80 ~ 90% Between, and control gas concentration lwevel below 0.1% and scatter illumination within the scope of 1500 ~ 5000Lx;(2) every batch of incubation time It is 30 ~ 40 days, is hardened to cap surface presentation paint sample gloss, cap, mature spore constantly comes out below cap, just It can harvest;(3) when three batches harvesting after the completion of, into cultivating bag supplement glucose, protein, lignin, continue culture to four batches, Five batches.
Insect prevention measure: the greenhouse for cultivating ganoderma lucidum, which is first divulged information, to be exposed to the sun, and quick lime deinsectization, ozonization are spilt in ground, and access door is set Set buffering, ventilation opening is isolated with 40 mesh gauzes around greenhouse, trap lamp is set in canopy, and uniformly install in canopy temperature, humidity, Carbon dioxide probe adjusts temperature, humidity and gas concentration lwevel for monitoring in real time, hard when finding ganoderma lucidum germ and pest Artificial treatment is held, mildew or pollution is found, removes in time, prevent expansion or cross-infection.
Embodiment 3
A kind of green soilless culture method of ganoderma lucidum, comprising the following steps:
Stock: (1) after impregnating fresh tea leaf 14 hours according to the ratio that solid-liquid ratio is 1:12 with 25 DEG C of water, 35 points are boiled Clock is cooled to room temperature, and with filtered through gauze, is boiled again by above-mentioned steps 1 time to gained tealeaf residue, obtains tea after merging filtered fluid twice Leaf extract;(2) tea extract obtained is added into PDA culture medium, ratio 0.08ml/g, is made and is mentioned containing tealeaves Take the PDA culture medium of liquid;(3) remaining tealeaf residue is dried, is crushed between 0.2 ~ 2.0cm;(4) bran for being 5 parts by parts by weight Pi Jiashui prewets after softening, and 100 parts of addition maple tree bits, 20 parts of tealeaf residue and 2 parts of gypsum stir evenly, and adds sucrose 0.8 Culture material is sufficiently stirred to obtain in part, and the water content of culture material controls between 60 ~ 65%, and pH value controls between 4 ~ 6.
The production of hybrid seeds: ganoderma lucidum fungus block is placed in the center of the PDA culture medium containing tea extract, 25 DEG C of constant temperature are protected from light culture, right Mycelia growth data is acquired.
Bacterium germination: (1) culture material for preparing is packed into material bag in and cultivating bag is made, through sterilizing at 0.15MPa, 130 DEG C 1.5h accesses strain obtained in the method for sterile working, and strain and culture medium are in close contact, in favor of being colonized and growing;(2) Cultivating bag after inoculation is moved into culturing room's culture, controls temperature between 18 ~ 25 DEG C, between humidity 55 ~ 65%, is protected from light culture 45 days, Ventilation 1 time daily after a week.
Out sesame manage: (1) when in cultivating bag culture medium avoid the occurrence of white or yellow protrusion pimple block, uncap, shift To the grid type cultivating stand pre-set, culture density is 200 Bang/㎡, controls temperature between 25 ~ 30 DEG C, humidity 80 ~ 90% Between, and control gas concentration lwevel below 0.1% and scatter illumination within the scope of 1500 ~ 5000Lx;(2) every batch of incubation time It is 30 ~ 40 days, is hardened to cap surface presentation paint sample gloss, cap, mature spore constantly comes out below cap, just It can harvest;(3) when three batches harvesting after the completion of, into cultivating bag supplement glucose, protein, lignin, continue culture to four batches, Five batches.
Insect prevention measure: the greenhouse for cultivating ganoderma lucidum, which is first divulged information, to be exposed to the sun, and quick lime deinsectization, ozonization are spilt in ground, and access door is set Set buffering, ventilation opening is isolated with 40 mesh gauzes around greenhouse, trap lamp is set in canopy, and uniformly install in canopy temperature, humidity, Carbon dioxide probe adjusts temperature, humidity and gas concentration lwevel for monitoring in real time, hard when finding ganoderma lucidum germ and pest Artificial treatment is held, mildew or pollution is found, removes in time, prevent expansion or cross-infection.
Comparative example 1 is set, difference from example 1 is that, tea extract is not added in culture medium;
Comparative example 2 is set, difference from example 1 is that, tealeaf residue is not added in culture material;
Comparing embodiment 1 ~ 3 and comparative example 1, the hypha growth condition of comparative example 2, as a result as shown in the table:
Note: mycelial growth rate (mm/d)=(average colony diameter -6)/(mycelia grows number of days * 2).
As shown above, mycelia growth rate, the growth potential for adding tea extract are improved, and colony edge is neat Degree, roundness are all good, and the concentration in embodiment 1 is optimal, it was demonstrated that the substance in tea extract can effectively facilitate the life of mycelia It is long.
Comparative example 4 ~ 6 is set, is commercially available ganoderma lucidum, the extraction of ganodenic acid is carried out to embodiment 1 ~ 3 and comparative example 4 ~ 6, is adopted With HPLC method measurement content, (high concentration ethanol liquid heating extraction, utilizes high performance liquid chromatography after extracting solution concentration after raw material crushes Instrument is detected, and makees standard items with ganoderic acid, obtains ganodenic acid content in raw material by absorbing peak area accordingly), as a result It is as shown in the table:
Ganoderma total triterpenes content (%) testing result table
Note: sample 1,2,3 is 1 sample of embodiment;Sample 4,5,6 is 2 sample of embodiment;Sample 7,8,9 is 3 sample of embodiment; Sample 10 is comparative example 4 Mount Huang 1 ganoderma lucidum sample;Sample 11 is 5 No. 1 sample of Zhejiang celestial being sesame of comparative example;Sample 12 is comparative example 6 No. 6 samples of river sesame;Test 1,2,3 is parallel test.
As shown above, commercially available spirit is higher than using the Ganoderma total triterpenes content of the ganoderma lucidum of cultivation of glossy ganoderma Bag Material of the present invention cultivation Sesame.
Specific embodiment described herein is only an example for the spirit of the invention.The neck of technology belonging to the present invention The technical staff in domain can make various modifications or additions to the described embodiments or replace by a similar method In generation, however, it does not deviate from the spirit of the invention or beyond the scope of the appended claims.

Claims (10)

1. a kind of green soilless culture method of ganoderma lucidum, which comprises the following steps:
Step 1: being added tea extract in bacterium culture medium, and by strain, constant temperature is protected from light culture at 24 ~ 28 DEG C;
Step 2: tealeaf residue is crushed, and is uniformly mixed with sawdust, wheat bran, is obtained culture material;
Step 3: being made cultivation Bag Material for culture material loading strain bag, cooling after the processing of high temperature and pressure moist heat sterilization, access Lucidum strain carries out 30 ~ 40d of culture;
Step 4: the ganoderma lucidum after step 3 culture is transferred in greenhouse and carries out out sesame management;
Step 5: mature fructification is harvested, and after the completion of three batches of culture harvestings, is added nitrogen source and carbon source nutriment, is continued Carry out four batches and five batches of culture, harvesting.
2. a kind of green soilless culture method of ganoderma lucidum according to claim 1, which is characterized in that planted in the step 2 The ingredient of training material includes following parts by weight: 60 ~ 100 parts of sawdust, 5 ~ 15 parts of wheat bran, 5 ~ 20 parts of tealeaf residue, 1 ~ 3 part of gypsum, sucrose 0.5 ~ 1.0 part.
3. a kind of green soilless culture method of ganoderma lucidum according to claim 1, which is characterized in that tea in the step 1 The preparation step of leaf extract includes: after impregnating tealeaves 10 ~ 14 hours with 20 ~ 25 DEG C of water, to boil 25 ~ 35 minutes, cooling, Filtering, boils gained tealeaf residue 1 ~ 2 time again, obtains tea extract after merging filtered fluid.
4. a kind of green soilless culture method of ganoderma lucidum according to claim 1, which is characterized in that tea in the step 2 The granular size of leaf slag is 0.2 ~ 2.0cm.
5. a kind of green soilless culture method of ganoderma lucidum according to claim 1, which is characterized in that the sawdust is maple Sawdust or chestnut sawdust.
6. a kind of green soilless culture method of ganoderma lucidum according to claim 2, which is characterized in that planted in the step 2 The processing of training material include: by wheat bran add water prewet sawdust is added after softening, tealeaf residue and land plaster stir evenly, add sugarcane Sugar stirs to obtain culture material.
7. a kind of green soilless culture method of ganoderma lucidum according to claim 6, which is characterized in that described to prewet when softening Between be 22 ~ 25 hours.
8. a kind of green soilless culture method of ganoderma lucidum according to claim 1, which is characterized in that planted in the step 3 Train the high temperature and pressure moist heat sterilization treatment conditions of Bag Material are as follows: 0.14 ~ 0.15MPa, the 1.5 ~ 2h or 100 that sterilizes at 125 ~ 130 DEG C ~ Sterilize 10 ~ 12h at 110 DEG C.
9. a kind of green soilless culture method of ganoderma lucidum according to claim 1, which is characterized in that go out in the step 4 The condition of sesame management are as follows: 25 DEG C ~ 30 DEG C of temperature, humidity 80% ~ 90%, gas concentration lwevel scatters illumination 1500- less than 0.1% 5000Lx。
10. a kind of green soilless culture method of ganoderma lucidum according to claim 1, which is characterized in that in the step 5 Nitrogen source and carbon source nutriment include glucose, protein, crude fibre.
CN201910631870.1A 2019-07-12 2019-07-12 A kind of green soilless culture method of ganoderma lucidum Pending CN110301286A (en)

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CN113796262A (en) * 2021-10-11 2021-12-17 广西壮族自治区农业科学院 Cultivation method of ganoderma lucidum with high ganoderma lucidum spore powder yield
CN113796262B (en) * 2021-10-11 2022-06-07 广西壮族自治区农业科学院 Cultivation method of ganoderma lucidum with high ganoderma lucidum spore powder yield

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