CN105493944A - Blueberry seedling tending substrate and preparation method thereof - Google Patents
Blueberry seedling tending substrate and preparation method thereof Download PDFInfo
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Abstract
Description
技术领域technical field
本发明涉及幼苗抚育基质技术领域,具体涉及一种蓝莓幼苗抚育基质及其制备方法。The invention relates to the technical field of seedling tending substrates, in particular to a blueberry seedling tending substrate and a preparation method thereof.
背景技术Background technique
蓝莓(blueberry)属于杜鹃花科(Ericaceae)越橘属(Vaccinium)植物,为多年生落叶或常绿灌木。其具有防止脑神经衰老、增强心脏功能、预防视力疲劳及防癌抗癌的独特功效,是联合国粮农组织推荐的五大健康水果之一。蓝莓做为一种新兴果树而具有巨大的经济效益和发展空间。蓝莓种苗繁育现多以容器育苗为主,要在容器中抚育1~3年待幼苗达到一定大小时再进行栽植。利用容器扶育蓝莓幼苗对提高蓝莓栽植成活率和实行蓝莓早期丰产等有重要作用。Blueberry (blueberry) belongs to Rhododendron (Ericaceae) Vaccinium (Vaccinium) plant, is perennial deciduous or evergreen shrub. It has the unique effects of preventing brain nerve aging, enhancing heart function, preventing visual fatigue, and preventing and fighting cancer. It is one of the five healthy fruits recommended by the Food and Agriculture Organization of the United Nations. As a new fruit tree, blueberry has huge economic benefits and development space. The breeding of blueberry seedlings is mostly based on container seedling cultivation, and it is necessary to raise them in containers for 1 to 3 years until the seedlings reach a certain size before planting. The use of containers to cultivate blueberry seedlings plays an important role in improving the survival rate of blueberry planting and implementing early harvest of blueberry.
蓝莓幼苗生长对抚育基质pH值要求较为严格,要求pH值为4.0~5.5;同时对通透性和保水性要求较高;幼苗生长需要在低供肥强度下有持续的营养供应。但蓝莓苗在容器中生长时间较长,容器内营养土的理化性状和养分供应能力等均会对根系的生长分布造成一定的限制。因此质轻、形状好且能满足蓝莓幼苗生长需求的基质是十分必要。The growth of blueberry seedlings has strict requirements on the pH value of the tending substrate, which requires a pH value of 4.0 to 5.5; at the same time, it has high requirements on permeability and water retention; seedling growth requires continuous nutrient supply under low fertilization intensity. However, blueberry seedlings grow in containers for a long time, and the physical and chemical properties and nutrient supply capacity of the nutrient soil in the container will cause certain restrictions on the growth and distribution of the root system. Therefore, a substrate that is light in weight, good in shape and can meet the growth needs of blueberry seedlings is very necessary.
目前常规的蓝莓幼苗抚育多以土壤为主体,通过添加一定量的有机材料来调配基质,通过喷灌方式进行浇水肥,这样易导致苗坨土结构紧实,引发生理性根腐病或导致苗木生长不良;同时育苗土坨过重也增加运输成本。At present, the conventional blueberry seedling tending is mostly based on the soil, adding a certain amount of organic materials to prepare the substrate, and watering and fertilizing through spray irrigation, which will easily lead to the compact structure of the seedling soil, causing physiological root rot or causing seedlings to grow Bad; At the same time, the overweight seedling soil lump also increases the transportation cost.
利用草炭为主要材料制作育苗基质是常用的方法。但我国草炭以低品位草本草炭为主,高质量的藓类草炭储量很少;我国裸露草炭地主要分布在东北山地平原和西北西南高原。近年来草炭被大量开采外运,使这一天然资源濒于枯竭,造成了湿地生态环境的严重恶化,限制草炭使用的压力越来越大,开发草炭的替代品变得越来越重要。It is a common method to use peat as the main material to make seedling substrate. However, the peat in my country is dominated by low-grade herbaceous peat, and the reserves of high-quality moss peat are very small; the bare peat land in my country is mainly distributed in the mountainous plains in the northeast and the plateau in the northwest and southwest. In recent years, a large amount of peat has been mined and exported, which has caused this natural resource to be on the verge of depletion, resulting in a serious deterioration of the wetland ecological environment. The pressure to limit the use of peat is increasing, and the development of substitutes for peat is becoming more and more important.
近年来各地均积极研发本土化的育苗基质,锯末、牛粪、醋糟、椰糠、木薯渣、菇渣等均被应用于的育苗中。在筛选不同作物生育需求的基质时,既可选用单一基质,也可将几种基质按一定比例复配而成。复配基质是由性质、结构、性能等不同的基质混合而成,在水分和肥料方面协调效果好于单一基质。但是复配基质选配的基质不同、选配的比例不同,复配基质的性能也不同,这将使得不同复配基质对作物的育苗效果也会不同。基于上述情况对于不同作物应当选取合适的基质以及合适的基质配比。In recent years, localized seedling substrates have been actively developed in various places. Sawdust, cow dung, vinegar grains, coconut peat, cassava residue, mushroom residue, etc. have all been used in seedling cultivation. When screening substrates for different crop growth needs, a single substrate can be selected, or several substrates can be compounded in a certain proportion. The compound substrate is a mixture of substrates with different properties, structures, and performances, and its coordination effect in terms of water and fertilizer is better than that of a single substrate. However, the selected substrates of the compound substrates are different, the proportions of the selection are different, and the properties of the compound substrates are also different, which will make the effects of different compound substrates on crop seedlings also different. Based on the above situation, suitable substrates and suitable substrate ratios should be selected for different crops.
哈茨木霉菌可以预防由腐霉菌、立枯丝核菌、镰刀菌、黑根霉等病原菌引起的植物根部病害。在植物根围生长并形成"保护罩",以防止根部病原真菌的侵染。并能产生刺激植物生长和诱导植物防御反应的化合物,改善根系的微环境,增强植物的长势和抗病能力,提高作物的产量和收益。将有益菌添加到蔬菜育苗基质中对培育无病壮苗起到较大作用。目前基质育苗在蔬菜生产中得到大面积推广应用并取得巨大的经济效益,而现对蓝莓幼苗抚育基质的研究较少,特别是添加生防菌的蓝莓幼苗抚育基质更少。Trichoderma harzianum can prevent plant root diseases caused by pathogens such as Pythium, Rhizoctonia solani, Fusarium, and Rhizopus niger. It grows around the roots of plants and forms a "protective shield" to prevent the infection of root pathogenic fungi. And can produce compounds that stimulate plant growth and induce plant defense responses, improve the microenvironment of the root system, enhance plant growth and disease resistance, and increase crop yield and income. Adding beneficial bacteria to the vegetable seedling substrate has a greater effect on cultivating disease-free and strong seedlings. At present, matrix seedlings have been widely used in vegetable production and have achieved huge economic benefits, but there are few studies on blueberry seedling tending substrates, especially blueberry seedling tending substrates added with biocontrol bacteria.
发明内容Contents of the invention
针对上述现有技术存在的不足,本发明提供一种蓝莓幼苗抚育基质及其制备方法。本发明的蓝莓幼苗抚育基质,取材方便,成本低,能促进蓝莓的生长,培育无病蓝莓壮苗。Aiming at the deficiencies in the above-mentioned prior art, the present invention provides a blueberry seedling tending substrate and a preparation method thereof. The blueberry seedling rearing substrate of the invention has the advantages of convenient materials and low cost, can promote the growth of blueberries, and cultivate disease-free and strong blueberry seedlings.
为实现上述目的,本发明采用如下技术方案:To achieve the above object, the present invention adopts the following technical solutions:
一种蓝莓幼苗抚育基质,由以下体积份数的原料组成:锯末与牛粪混合发酵物400~500份,草炭150~200份,珍珠岩70~100份,蛭石30~50份,棕壤土200~300份,棉隆0.1~0.2份,硫磺粉0.1~0.2份,木霉菌0.1~0.3份,控释肥1.5~2.5份。A blueberry seedling rearing substrate, which is composed of the following raw materials in parts by volume: 400-500 parts of mixed fermentation product of sawdust and cow dung, 150-200 parts of peat, 70-100 parts of perlite, 30-50 parts of vermiculite, and brown loam 200-300 parts, cotton 0.1-0.2 parts, sulfur powder 0.1-0.2 parts, Trichoderma 0.1-0.3 parts, controlled-release fertilizer 1.5-2.5 parts.
优选的,所述一种蓝莓幼苗抚育基质,由以下体积份数的原料组成:锯末与牛粪混合发酵物450份,草炭150份,珍珠岩100份,蛭石50份,棕壤土250份,棉隆0.16份,硫磺粉0.13份,木霉菌0.2份,控释肥1.8份。Preferably, the substrate for raising blueberry seedlings is composed of the following raw materials in parts by volume: 450 parts of mixed fermentation product of sawdust and cow dung, 150 parts of peat, 100 parts of perlite, 50 parts of vermiculite, 250 parts of brown loam, 0.16 parts of cotton long, 0.13 parts of sulfur powder, 0.2 parts of Trichoderma, 1.8 parts of controlled release fertilizer.
优选的,所述一种蓝莓幼苗抚育基质,它由以下体积份数的原料组成:锯末与牛粪混合发酵物500份,草炭150份,珍珠岩100份,蛭石50份,棕壤土200份,棉隆0.15份,硫磺粉0.12份,木霉菌0.15份,控释肥2.0份。Preferably, the substrate for raising blueberry seedlings is composed of the following raw materials in parts by volume: 500 parts of sawdust and cow dung mixed fermentation product, 150 parts of peat, 100 parts of perlite, 50 parts of vermiculite, and 200 parts of brown loam , 0.15 parts of cotton long, 0.12 parts of sulfur powder, 0.15 parts of Trichoderma, 2.0 parts of controlled release fertilizer.
优选的,所述锯末为针叶木锯末。Preferably, the sawdust is softwood sawdust.
本发明还公开了一种蓝莓幼苗抚育基质的制备方法,它包括以下制备步骤:The invention also discloses a preparation method of the blueberry seedling tending substrate, which comprises the following preparation steps:
(1)将锯末与牛粪混合发酵物、草炭、珍珠岩、蛭石、棕壤土按所述比例掺拌,得混合基质。(1) Mix sawdust with cow dung, peat, perlite, vermiculite, and brown loam according to the stated ratio to obtain a mixed substrate.
(2)熏蒸消毒:向所述混合基质中添加棉隆并混匀,覆盖不透气的塑料膜进行密闭熏蒸,消毒完成后,揭膜通气并疏松基质,充分换气。(2) Fumigation and disinfection: add cotton wool to the mixed matrix and mix evenly, cover with an airtight plastic film for airtight fumigation, after the disinfection is completed, remove the film to ventilate and loosen the matrix, and fully ventilate.
(3)向经熏蒸消毒后的混合基质中添加硫磺粉,调至基质pH值至4.5~4.8。(3) Add sulfur powder to the fumigated mixed matrix to adjust the pH value of the matrix to 4.5-4.8.
(4)装钵前加入所述比例的木霉菌和控释肥并混匀,即得蓝莓幼苗抚育基质。(4) Adding Trichoderma and controlled-release fertilizer in said ratio and mixing before potting, to obtain the blueberry seedling rearing substrate.
优选的,所述锯末与牛粪混合发酵物的制备方法:锯末和牛粪按2~3︰1的比例配比,调节水分达到60%~65%,然后按每平方米锯末加2kg腐熟剂,混匀后进行好氧堆肥发酵,温度达42℃~45℃进行第一次翻堆,待温度达到55℃以上后每隔3~5天翻一次堆,连续翻堆3~4次,直至发酵堆温度开始下降后,以后每7~10天翻一次,待堆料自然降温接近外界温度后即制得锯末与牛粪混合发酵物。Preferably, the preparation method of the mixed fermentation product of sawdust and cow dung: mix sawdust and cow dung in a ratio of 2 to 3:1, adjust the water content to 60% to 65%, and then add 2kg of decomposing agent per square meter of sawdust, After mixing, carry out aerobic composting and fermentation. The temperature reaches 42 ℃ ~ 45 ℃ for the first turning over. After the temperature reaches above 55 ℃, turn over the compost every 3 to 5 days, and turn the compost 3 to 4 times continuously until fermentation After the temperature of the heap begins to drop, turn it over every 7 to 10 days. After the natural cooling of the heap is close to the outside temperature, a mixed fermentation product of sawdust and cow dung is produced.
优选的,所述步骤(2)中覆盖不透气的塑料膜密闭熏蒸15-20天,揭膜通气并疏松基质2~3次,充分换气10天以上。Preferably, in the step (2), the airtight plastic film is covered with airtight fumigation for 15-20 days, the film is removed for ventilation and the matrix is loosened for 2-3 times, and the ventilation is sufficient for more than 10 days.
优选的,所述锯末为针叶木锯末。Preferably, the sawdust is softwood sawdust.
优选的,所述木霉菌为不低于3亿cfu/克的哈茨木霉。Preferably, the Trichoderma is not less than 300 million cfu/g Trichoderma harzianum.
优选的,所述控释肥为控释期9个月,N-P2O5-K2O为19-9-11的包膜控释肥。Preferably, the controlled-release fertilizer is a coated controlled-release fertilizer with a controlled-release period of 9 months and an NP 2 O 5 -K 2 O ratio of 19-9-11.
基质有部分与土壤相似的功能,包括固定、持水、通气、缓冲以及提供部分营养,而化肥仅是指能够为植物提供营养成分的物质。Substrate has some functions similar to soil, including fixation, water holding, aeration, buffering and providing some nutrients, while chemical fertilizers only refer to substances that can provide nutrients for plants.
锯末与牛粪混合发酵物是蓝莓幼苗抚育基质主要的成分,在基质中占比最多,起着固定植株、稳定基质酸碱度、为幼苗提供养分的作用。一方面采用针叶木锯末可以将基质pH值维持在较低的范围,有助于蓝莓幼苗的生长,另一方面,通过发酵温度达到55℃以上时,能够杀灭牛粪中的大量病原菌、寄生虫卵、杂草种子等,最初对锯末和牛粪混合发酵物的翻堆频率较高,能够对基质起到多次、充分发酵,并有效地保存了牛粪中的养分,对基质起到充分消毒灭菌的作用。发酵后期,降低翻堆频率,有助于形成比较稳定和腐熟的基质成分。同时由于针叶木中含有对真菌有拮抗作用的蒎烯和松香酸等萜类有机物质,可以减少蓝莓幼苗病虫害的发生,因此,采用锯末与牛粪混合发酵物作为主要基质成分能够稳定蓝莓幼苗生长环境,对幼苗的生长起到灭菌消毒的作用,有利于培育无病的蓝莓壮苗。The mixed fermentation product of sawdust and cow dung is the main component of the blueberry seedling rearing substrate, accounting for the largest proportion in the substrate, which plays the role of fixing plants, stabilizing the pH of the substrate, and providing nutrients for the seedlings. On the one hand, the use of coniferous wood sawdust can maintain the pH value of the substrate in a low range, which is conducive to the growth of blueberry seedlings; Insect eggs, weed seeds, etc., the initial turning frequency of the mixed fermentation product of sawdust and cow dung is relatively high, which can fully ferment the substrate for many times, and effectively preserve the nutrients in the cow dung, and fully play a role in the substrate. The role of disinfection and sterilization. In the later stage of fermentation, reducing the frequency of compost turning helps to form a relatively stable and decomposed matrix component. At the same time, because coniferous wood contains terpenoid organic substances such as pinene and abietic acid, which have antagonistic effect on fungi, it can reduce the occurrence of diseases and insect pests of blueberry seedlings. Therefore, the mixed fermentation product of sawdust and cow dung as the main matrix component can stabilize the growth of blueberry seedlings. The environment plays a role of sterilization and disinfection for the growth of seedlings, which is conducive to the cultivation of disease-free blueberry seedlings.
蓝莓属于寡营养植物,植株内氮、磷、钾、钙、镁含量很低,其根系多为须根,且主根不明显。本申请采用锯末与牛粪混合发酵物、草炭、珍珠岩、蛭石、棕壤土、控释肥搭配使用,有机质成分占15~25%,总养分含量2~2.5%,能够持续和缓慢为蓝莓幼苗提供所需的正常养分,而不会因养分过量而使幼苗收到伤害甚至整株死亡。同时,蓝莓对基质的要求本身就需要具有一定的酸碱性,珍珠岩、蛭石和棕壤土的pH值比炉渣、河沙等常用基质的pH偏低,经搭配混合后体系的缓冲能力较强,有利于稳定后期基质的pH值;其次,珍珠岩和蛭石的孔隙度较高,通气排水能力强,经搭配使用后,能使基质的总孔隙度达到60-70%,有利于蓝莓幼苗的生根,降低基质容重,降低企业的运输成本。Blueberry is an oligotrophic plant. The content of nitrogen, phosphorus, potassium, calcium, and magnesium in the plant is very low. The roots are mostly fibrous roots, and the main root is not obvious. This application uses sawdust and cow dung mixed fermentation products, peat, perlite, vermiculite, brown loam, and controlled-release fertilizers. The organic matter accounts for 15-25%, and the total nutrient content is 2-2.5%, which can sustainably and slowly grow blueberries. The seedlings provide the normal nutrients they need, and the seedlings will not be damaged or even the whole plant will die due to excessive nutrients. At the same time, blueberries require a certain acidity and alkalinity for the substrate itself. The pH value of perlite, vermiculite and brown loam is lower than that of common substrates such as slag and river sand, and the buffering capacity of the system after mixing , which is conducive to stabilizing the pH value of the substrate in the later stage; secondly, perlite and vermiculite have high porosity and strong ventilation and drainage capabilities. After being used together, the total porosity of the substrate can reach 60-70%, which is beneficial to blueberry seedlings rooting, reduce the bulk density of the substrate, and reduce the transportation cost of the enterprise.
木霉菌对植物的土传病害具有防治作用,但基质的pH值对木霉菌丝的生长及孢子的形成有较为明显的影响。一般来说pH5-7.5的范围内菌丝生长速度最快,当pH低于4.5时,菌丝生长明显变慢,而适宜蓝莓幼苗生长的基质pH值范围要求是4.0~5.5,由于栽培后期由于有机质的分解,根系分泌,微生物影响等等,基质的pH值会略有升高,因此,栽培初期基质的pH值设定会偏低,也略低于木霉菌的生长适宜条件。但通过实施过程发现,加入硫磺粉调节pH后的基质对木霉菌的生长影响不大,两者相容性好。蓝莓的生根过程很容易受到真菌侵染,幼苗易腐烂,通过木霉菌的添加与基质中的针叶木锯末的相互协同作用,可以对蓝莓幼苗根部的真菌起到明显抑制作用,大大提高蓝莓幼苗的成活率。Trichoderma can prevent and control soil-borne diseases of plants, but the pH value of the substrate has a more obvious effect on the growth of Trichoderma mycelium and the formation of spores. Generally speaking, the growth rate of mycelium is the fastest in the range of pH 5-7.5. When the pH is lower than 4.5, the growth of mycelium is obviously slowed down. The decomposition of organic matter, root secretion, microbial influence, etc., will slightly increase the pH value of the substrate. Therefore, the pH value of the substrate at the initial stage of cultivation will be set on the low side, which is also slightly lower than the suitable growth conditions for Trichoderma. However, it was found through the implementation process that adding sulfur powder to adjust the pH of the substrate had little effect on the growth of Trichoderma, and the compatibility between the two was good. The rooting process of blueberries is very susceptible to fungal infection, and the seedlings are perishable. The addition of Trichoderma and the synergistic effect of conifer sawdust in the substrate can significantly inhibit the fungi at the roots of blueberry seedlings, greatly improving the growth rate of blueberry seedlings. survival rate.
本发明的有益效果是:The beneficial effects of the present invention are:
(1)本发明的蓝莓幼苗抚育基质容重0.4~0.6g/cm3,总孔隙度60%~70%,有机质15%~25%,pH值4.5~4.8,每克含木霉菌cfu>1×105,总养分含量2%~2.5%,持续供肥期可达6个月以上。(1) The blueberry seedling tending substrate of the present invention has a bulk density of 0.4 to 0.6 g/cm 3 , a total porosity of 60% to 70%, an organic matter of 15% to 25%, a pH value of 4.5 to 4.8, and contains Trichoderma cfu>1× per gram 10 5 , the total nutrient content is 2% to 2.5%, and the continuous fertilization period can reach more than 6 months.
(2)本发明利用锯末与牛粪混合发酵物、草炭及棕壤土配制配制成的蓝莓幼苗抚育基质,能够改善基质理化性状,减少单一基质的不良反应,促进蓝莓的生长,减少了草炭的使用量,能够在保证蓝莓幼苗品质的前提下降低了生产成本,原料来源广泛,价格低廉,节约资源,实现了锯末和牛粪的资源化利用,也降低了对草炭基质的依赖,缓解了草炭的开采压力及由此而造成的环境压力,具有明显的社会效益、经济效益和环境生态效益。这样为本发明的推广应用奠定了基础。(2) The present invention utilizes sawdust and cow dung mixed fermented product, peat and brown loam to prepare blueberry seedling tending substrate, which can improve the physical and chemical properties of the substrate, reduce adverse reactions of a single substrate, promote the growth of blueberries, and reduce the use of peat The quantity can reduce the production cost under the premise of ensuring the quality of blueberry seedlings. The source of raw materials is wide, the price is low, and resources are saved. The resource utilization of sawdust and cow dung is realized, and the dependence on peat substrate is also reduced, which eases the exploitation of peat. The pressure and the resulting environmental pressure have obvious social benefits, economic benefits and environmental ecological benefits. This lays the foundation for the popularization and application of the present invention.
(3)由于熏蒸之后的基质会处于一个生物真空状态,有两个月的危险期,非常容易受到病菌的再次侵害,本发明通过在熏蒸之后调节pH值,再添加木霉菌,有效地避免了危险期中病菌的侵害,并且由于木霉菌与硫磺粉之间相容性好,不会相互影响,大大提高蓝莓幼苗的成活率。(3) Because the matrix after fumigation will be in a biological vacuum state, there is a dangerous period of two months, and it is very easy to be invaded again by germs. The present invention effectively avoids the problem by adjusting the pH value after fumigation and adding Trichoderma. The invasion of pathogens in the dangerous period, and because of the good compatibility between Trichoderma and sulfur powder, will not affect each other, greatly improving the survival rate of blueberry seedlings.
(4)本发明的基质容重低,降低运输成本,采用复合材料能够改善蓝莓抚育幼苗基质的理化性状,减少单一基质的不良反应,消除基质中携带的杂草种子、病菌、虫卵等,减轻苗期病害,添加生防菌,有利于培育无病的蓝莓壮苗,促进蓝莓的生长,与目前蓝莓传统幼苗抚育相比,该发明筛选的复合基质理化性状能满足蓝莓生长要求,使植株生长一致,植株健壮、病虫害少,整体性状优良,植株形态指标综合评价指数高。(4) The matrix bulk density of the present invention is low, reduces transportation cost, adopts composite material to be able to improve the physical and chemical properties of blueberry tending seedling matrix, reduces the untoward reaction of single matrix, eliminates the weed seed, germ, worm egg etc. carried in the matrix, alleviates Seedling disease, adding bio-control bacteria is conducive to the cultivation of disease-free blueberry seedlings and promotes the growth of blueberries. Compared with the current traditional blueberry seedling tending, the physical and chemical properties of the composite matrix screened by the invention can meet the growth requirements of blueberries and make the plants grow Consistent, strong plants, less pests and diseases, excellent overall traits, high comprehensive evaluation index of plant morphology indicators.
具体实施方式detailed description
下面结合实施例对本发明进一步说明。Below in conjunction with embodiment the present invention is further described.
以下所述木霉菌购自北京拜沃生物技术有限公司,批号:20130410。The Trichoderma described below was purchased from Beijing Byward Biotechnology Co., Ltd., batch number: 20130410.
实施例1Example 1
一种蓝莓幼苗抚育基质,由以下体积份数的原料组成:针叶木锯末与牛粪混合发酵物400份,草炭200份,珍珠岩70份,蛭石30份,棕壤土300份,棉隆0.18份,硫磺粉0.15份,木霉菌0.22份,控释肥2.1份。A blueberry seedling rearing substrate, which is composed of the following raw materials in parts by volume: 400 parts of conifer sawdust and cow dung mixed fermentation product, 200 parts of peat, 70 parts of perlite, 30 parts of vermiculite, 300 parts of brown loam, and 0.18 parts of cotton long 0.15 parts of sulfur powder, 0.22 parts of Trichoderma, 2.1 parts of controlled release fertilizer.
一种蓝莓幼苗抚育基质的制备方法,它包括以下制备步骤:A kind of preparation method of blueberry seedling tending substrate, it comprises the following preparation steps:
(1)针叶木锯末与牛粪混合发酵物的制备:针叶木锯末和牛粪按2︰1的比例配比,调节水分达到60%~65%,然后按每平方米针叶木锯末加2kg腐熟剂,混匀后进行好氧堆肥发酵,温度达42℃~45℃进行第一次翻堆,待温度达到55℃以上后每隔3~5天翻一次堆,连续翻堆3~4次,直至发酵堆温度开始下降后,以后每7~10天翻一次,待堆料自然降温接近外界温度后即制得针叶木锯末与牛粪混合发酵物。(1) Preparation of mixed fermented product of coniferous wood sawdust and cow dung: mix coniferous wood sawdust and cow dung in a ratio of 2:1, adjust the water content to 60% to 65%, and then add 2kg decomposing agent per square meter of coniferous wood sawdust , after mixing, carry out aerobic composting and fermentation, turn the compost for the first time when the temperature reaches 42°C-45°C, turn the pile once every 3-5 days after the temperature reaches above 55°C, and turn the pile 3-4 times continuously until After the temperature of the fermentation heap begins to drop, it will be turned every 7 to 10 days thereafter. After the natural cooling of the heap is close to the outside temperature, the mixed fermented product of coniferous wood sawdust and cow dung is obtained.
(2)将所述锯末与牛粪混合发酵物、草炭、珍珠岩、蛭石、棕壤土按所述比例掺拌,得混合基质。(2) The sawdust is mixed with cow dung mixed fermented product, peat, perlite, vermiculite, and brown loam according to the stated ratio to obtain a mixed substrate.
(3)向所述混合基质中添加棉隆并混匀,覆盖不透气的塑料膜密闭熏蒸15-20天,消毒完成后,揭膜通气并疏松基质2~3次,充分换气10天以上。(3) Add cotton wool to the mixed matrix and mix well, cover with an airtight plastic film and fumigate for 15-20 days, after the disinfection is completed, remove the film to ventilate and loosen the matrix for 2-3 times, and fully ventilate for more than 10 days .
(4)向经熏蒸消毒后的混合基质中添加硫磺粉,调至基质pH值至4.5~4.8。(4) Add sulfur powder to the fumigated mixed matrix to adjust the pH value of the matrix to 4.5-4.8.
(5)装钵前加入所述比例的木霉菌和控释肥并混匀,即得蓝莓幼苗抚育基质。(5) Add the Trichoderma and controlled-release fertilizer in the stated ratio and mix before filling the pot to obtain the blueberry seedling rearing substrate.
实施例2Example 2
一种蓝莓幼苗抚育基质,由以下体积份数的原料组成:针叶木锯末与牛粪混合发酵物450份,草炭150份,珍珠岩100份,蛭石50份,棕壤土250份,棉隆0.16份,硫磺粉0.13份,木霉菌0.2份,控释肥1.8份。A kind of blueberry seedling tending substrate, which is composed of the following raw materials in parts by volume: 450 parts of conifer sawdust and cow dung mixed fermentation product, 150 parts of peat, 100 parts of perlite, 50 parts of vermiculite, 250 parts of brown loam, and 0.16 parts of cotton long part, 0.13 part of sulfur powder, 0.2 part of Trichoderma, 1.8 part of controlled-release fertilizer.
一种蓝莓幼苗抚育基质的制备方法,它包括以下制备步骤:A kind of preparation method of blueberry seedling tending substrate, it comprises the following preparation steps:
(1)针叶木锯末与牛粪混合发酵物的制备:针叶木锯末和牛粪按3︰1的比例配比,调节水分达到60%~65%,然后按每平方米针叶木锯末加2kg腐熟剂,混匀后进行好氧堆肥发酵,温度达42℃~45℃进行第一次翻堆,待温度达到55℃以上后每隔3~5天翻一次堆,连续翻堆3~4次,直至发酵堆温度开始下降后,以后每7~10天翻一次,待堆料自然降温接近外界温度后即制得针叶木锯末与牛粪混合发酵物。(1) Preparation of mixed fermented product of coniferous wood sawdust and cow dung: mix coniferous wood sawdust and cow dung in a ratio of 3:1, adjust the water content to 60% to 65%, and then add 2kg of decomposing agent per square meter of coniferous wood sawdust , after mixing, carry out aerobic composting and fermentation, turn the compost for the first time when the temperature reaches 42°C-45°C, turn the pile once every 3-5 days after the temperature reaches above 55°C, and turn the pile 3-4 times continuously until After the temperature of the fermentation heap begins to drop, it will be turned every 7 to 10 days thereafter. After the natural cooling of the heap is close to the outside temperature, the mixed fermented product of coniferous wood sawdust and cow dung is obtained.
(2)将所述锯末与牛粪混合发酵物、草炭、珍珠岩、蛭石、棕壤土按所述比例掺拌,得混合基质。(2) The sawdust is mixed with cow dung mixed fermented product, peat, perlite, vermiculite, and brown loam according to the stated ratio to obtain a mixed substrate.
(3)向所述混合基质中添加棉隆并混匀,覆盖不透气的塑料膜密闭熏蒸15-20天,消毒完成后,揭膜通气并疏松基质2~3次,充分换气10天以上。(3) Add cotton wool to the mixed matrix and mix well, cover with an airtight plastic film and fumigate for 15-20 days, after the disinfection is completed, remove the film to ventilate and loosen the matrix for 2-3 times, and fully ventilate for more than 10 days .
(4)向经熏蒸消毒后的混合基质中添加硫磺粉,调至基质pH值至4.5~4.8。(4) Add sulfur powder to the fumigated mixed matrix to adjust the pH value of the matrix to 4.5-4.8.
(5)装钵前加入所述比例的木霉菌和控释肥并混匀,即得蓝莓幼苗抚育基质。(5) Add the Trichoderma and controlled-release fertilizer in the stated ratio and mix before filling the pot to obtain the blueberry seedling rearing substrate.
实施例3Example 3
它由以下体积份数的原料组成:锯末与牛粪混合发酵物500份,草炭150份,珍珠岩100份,蛭石50份,砂棕壤土200份,棉隆0.15份,硫磺粉0.12份,木霉菌0.15份,控释肥2.0份。It consists of the following raw materials in parts by volume: 500 parts of sawdust and cow dung mixed fermentation product, 150 parts of peat, 100 parts of perlite, 50 parts of vermiculite, 200 parts of sandy brown loam, 0.15 parts of cotton long, 0.12 parts of sulfur powder, Trichoderma 0.15 parts, controlled release fertilizer 2.0 parts.
一种蓝莓幼苗抚育基质的制备方法,它包括以下制备步骤:A kind of preparation method of blueberry seedling tending substrate, it comprises the following preparation steps:
(1)针叶木锯末与牛粪混合发酵物的制备:针叶木锯末和牛粪按2.5︰1的比例配比,调节水分达到60%~65%,然后按每平方米针叶木锯末加2kg腐熟剂,混匀后进行好氧堆肥发酵,温度达42℃~45℃进行第一次翻堆,待温度达到55℃以上后每隔3~5天翻一次堆,连续翻堆3~4次,直至发酵堆温度开始下降后,以后每7~10天翻一次,待堆料自然降温接近外界温度后即制得针叶木锯末与牛粪混合发酵物。(1) Preparation of mixed fermented product of coniferous wood sawdust and cow dung: mix coniferous wood sawdust and cow dung at a ratio of 2.5:1, adjust the water content to 60% to 65%, and then add 2kg of decomposing agent per square meter of coniferous wood sawdust , after mixing, carry out aerobic composting and fermentation, turn the compost for the first time when the temperature reaches 42°C-45°C, turn the pile once every 3-5 days after the temperature reaches above 55°C, and turn the pile 3-4 times continuously until After the temperature of the fermentation heap begins to drop, it will be turned every 7 to 10 days thereafter. After the natural cooling of the heap is close to the outside temperature, the mixed fermented product of coniferous wood sawdust and cow dung is obtained.
(2)将所述锯末与牛粪混合发酵物、草炭、珍珠岩、蛭石、棕壤土按所述比例掺拌,得混合基质。(2) The sawdust is mixed with cow dung mixed fermented product, peat, perlite, vermiculite, and brown loam according to the stated ratio to obtain a mixed substrate.
(3)向所述混合基质中添加棉隆并混匀,覆盖不透气的塑料膜密闭熏蒸15-20天,消毒完成后,揭膜通气并疏松基质2~3次,充分换气10天以上。(3) Add cotton wool to the mixed matrix and mix well, cover with an airtight plastic film and fumigate for 15-20 days, after the disinfection is completed, remove the film to ventilate and loosen the matrix for 2-3 times, and fully ventilate for more than 10 days .
(4)向经熏蒸消毒后的混合基质中添加硫磺粉,调至基质pH值至4.5~4.8。(4) Add sulfur powder to the fumigated mixed matrix to adjust the pH value of the matrix to 4.5-4.8.
(5)装钵前加入所述比例的木霉菌和控释肥并混匀,即得蓝莓幼苗抚育基质。(5) Add the Trichoderma and controlled-release fertilizer in the stated ratio and mix before filling the pot to obtain the blueberry seedling rearing substrate.
对照例(CK)Control (CK)
将棕壤土和草炭按体积比为2:1比例混合均匀,根据土壤pH值测定结果添加硫磺粉使土壤pH值在4.5~4.8之间得到草炭蓝莓幼苗抚育基质。Mix brown loam soil and peat at a volume ratio of 2:1, and add sulfur powder according to the soil pH value measurement results to make the soil pH value between 4.5 and 4.8 to obtain peat and blueberry seedling tending substrate.
实施例说明:Example Description:
1.不同锯末混配蓝莓幼苗抚育基质pH值变化。1. Changes in the pH value of the rearing substrate for blueberry seedlings mixed with different sawdust.
2013年在黄岛区潘家庄基地做了不同锯末材料添加物对蓝莓幼苗抚育基质pH值的变化,方法为:采用砂棕壤土、锯末和草炭为主要原料,利用稀硫酸调节基质土壤pH值至4.5。草炭用量为体积的20%不变,根据锯末的用量调节砂棕壤土用量。不同锯末混配蓝莓幼苗抚育基质pH值变化如表1。In 2013, at the Panjiazhuang base in Huangdao District, the pH value of the blueberry seedling rearing substrate was changed by different sawdust material additives. The method was: using sandy brown loam, sawdust and peat as the main raw materials, and using dilute sulfuric acid to adjust the pH value of the substrate soil to 4.5 . The amount of peat is constant at 20% of the volume, and the amount of sandy brown loam is adjusted according to the amount of sawdust. Table 1 shows the pH value changes of different sawdust mixed blueberry seedling rearing substrates.
表1不同锯末混配蓝莓幼苗抚育基质pH值变化Table 1 Changes in pH value of blueberry seedling rearing substrate mixed with different sawdust
从结果可看出:针叶木锯末在占40%时在栽苗后30天、90天、150天基质pH值分别升高-0.1、0、0.2,对基质pH值影响较小。阔叶木锯末在占40%时在栽苗后30天、90天、150天基质pH值分别升高0.2、0.4、0.9,其中在150天后基质pH值达5.6,已超过蓝莓生长适合pH值4~5.5范围。表明:选用针叶木锯末做蓝莓幼苗抚育基质材料优于阔叶木锯末。It can be seen from the results that when the conifer sawdust accounted for 40%, the substrate pH value increased by -0.1, 0, 0.2 at 30 days, 90 days, and 150 days after planting seedlings, and had little effect on the substrate pH value. When the broad-leaved wood sawdust accounted for 40%, the pH value of the substrate increased by 0.2, 0.4, and 0.9 at 30 days, 90 days, and 150 days after planting seedlings, and the pH value of the substrate reached 5.6 after 150 days, which has exceeded the pH value of 4, which is suitable for blueberry growth. ~5.5 range. It shows that the choice of coniferous wood sawdust as substrate material for blueberry seedling tending is better than that of broad-leaved wood sawdust.
2.配制得到的蓝莓幼苗抚育基质的基本理化性质。2. The basic physical and chemical properties of the prepared blueberry seedling rearing substrate.
2.1基质原料的基本理化性质:测得基质原料的基本理化性质如表2。2.1 Basic physical and chemical properties of matrix raw materials: the measured basic physical and chemical properties of matrix raw materials are shown in Table 2.
表2不同基质原料的基本理化性质Table 2 Basic physical and chemical properties of different matrix materials
2.2不同配比基质理化性状:按以上介绍的实施例的配制基质的蓝莓幼苗抚育基质的基本理化性质测定如表3。2.2 Physical and chemical properties of substrates with different ratios: Table 3 shows the basic physical and chemical properties of the substrates prepared for blueberry seedlings according to the above-mentioned examples.
表3不同配比基质理化性状Table 3 Physicochemical properties of matrix with different proportions
3.木霉菌与硫磺粉的相容性3. Compatibility of Trichoderma and sulfur powder
硫磺粉具有调节肥料及土壤PH的作用,木霉菌对蓝莓的土传病害具有防治作用,两者同时使用的情况下,研究硫磺粉对木霉菌的影响。采用以[硫磺粉/g:木霉菌可湿性粉剂/g:基质/mL]=1:1:1000的比例将硫磺粉于基质混合均匀后加入营养钵(直径9cm);以[木霉菌可湿性粉剂/g:基质/mL]=1:1000的比例混合均匀加入营养钵作为对照;两组处理各设置3个重复。五天后检测各个处理中木霉菌在每克基质中的定植数(cfu),通过cfu判断二者的相容性。结果如表4。Sulfur powder can regulate fertilizer and soil pH, and Trichoderma can prevent and control soil-borne diseases of blueberries. When the two are used at the same time, the effect of sulfur powder on Trichoderma is studied. Use [sulfur powder/g: Trichoderma wettable powder/g: substrate/mL] = 1:1:1000 to mix the sulfur powder with the substrate and add it to the nutrition bowl (diameter 9cm); Powder/g: matrix/mL] = 1:1000, mixed evenly and added to the nutrition bowl as a control; two groups of treatments were set up with 3 replicates. Five days later, the colonization number (cfu) of Trichoderma per gram of matrix in each treatment was detected, and the compatibility of the two was judged by cfu. The results are shown in Table 4.
表4木霉菌在基质中的生长情况The growth situation of table 4 Trichoderma in substrate
结果表明:硫磺粉对木霉菌的生长影响不大,二者之间相容性好。在田间使用过程中,二者混合使用的情况下不影响木霉菌的生长。The results showed that sulfur powder had little effect on the growth of Trichoderma, and the compatibility between the two was good. In the process of field use, the mixed use of the two will not affect the growth of Trichoderma.
4.对蓝莓成苗率的影响4. Effect on the seedling rate of blueberry
将实施例1~3、对照例配制的蓝莓幼苗抚育基质用于蓝莓育苗,相同条件下成活率统计表见表5。The blueberry seedling rearing substrates prepared in Examples 1-3 and the comparative example were used for blueberry seedling cultivation. See Table 5 for the statistics of the survival rate under the same conditions.
表5不同实施例对蓝莓成苗率的影响The impact of different embodiments of table 5 on blueberry seedling growth rate
通过表5可以看出,实施例1至实施例3的成苗率较高,与对照例有明显差异。实施例3根部发病率比对照低4倍,成苗率比对照高近10%。As can be seen from Table 5, the seedling rate of Embodiment 1 to Embodiment 3 is higher, and there are significant differences with the comparative example. In Example 3, the root disease rate is 4 times lower than that of the control, and the seedling rate is nearly 10% higher than that of the control.
5.对蓝莓幼苗生长发育的影响5. Effects on the growth and development of blueberry seedlings
在青岛市黄岛区潘家庄基地利用不同实施例在5月24日利用穴盘苗上12cm钵,蓝莓幼苗在钵内生长150d形态指标综合评价指数见表6。In Panjiazhuang Base, Huangdao District, Qingdao City, different embodiments were used to put the plug seedlings on a 12cm pot on May 24, and the comprehensive evaluation index of the morphological indicators of the blueberry seedlings grown in the pot for 150 days is shown in Table 6.
表6不同实施例对蓝莓幼苗生长形态的影响The impact of different embodiments of table 6 on the growth form of blueberry seedlings
通过表6可以看出,实施例1至实施例3的蓝莓茎粗、株高,甚至部分指标超过对照例。It can be seen from Table 6 that the blueberry stems and plant heights of Examples 1 to 3 exceed those of the comparative example.
由此可见,本发明的基质容重低,采用复合材料能够改善蓝莓抚育幼苗基质的理化性状,减少单一基质的不良反应,消除基质中携带的杂草种子、病菌、虫卵等,添加生防菌,有利于培育无病的蓝莓壮苗,促进蓝莓的生长,与目前蓝莓传统幼苗抚育相比,该发明筛选的复合基质理化性状能满足蓝莓生长要求,使植株生长一致,植株健壮、病虫害少,整体性状优良,植株形态指标综合评价指数高。而且原料来源广泛,价格低廉,节约资源,具有明显的社会效益、经济效益和环境生态效益。It can be seen that the matrix of the present invention has low bulk density, and the use of composite materials can improve the physical and chemical properties of the blueberry tending seedling matrix, reduce adverse reactions of a single matrix, eliminate weed seeds, germs, insect eggs, etc. carried in the matrix, and add bio-control bacteria , is conducive to the cultivation of disease-free strong blueberry seedlings, and promotes the growth of blueberries. Compared with the current traditional blueberry seedling tending, the physical and chemical properties of the composite matrix screened by the invention can meet the growth requirements of blueberries, so that the plants grow consistently, the plants are strong, and there are few pests and diseases. The overall traits are excellent, and the comprehensive evaluation index of plant morphological indicators is high. Moreover, the source of raw materials is wide, the price is low, resources are saved, and it has obvious social benefits, economic benefits and environmental ecological benefits.
最后应当说明的是,以上实施例仅用以说明本发明的技术方案,而非对本发明保护范围的限制,尽管参照较佳实施例对本发明作了详细地说明,本领域的普通技术人员应当理解,可以对本发明的技术方案进行修改或者等同替换,而不脱离本发明技术方案的实质和范围。Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than limiting the protection scope of the present invention, although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand , the technical solution of the present invention may be modified or equivalently replaced without departing from the spirit and scope of the technical solution of the present invention.
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