CN103609441A - Blueberry seedling culturing method - Google Patents

Blueberry seedling culturing method Download PDF

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Publication number
CN103609441A
CN103609441A CN201310555042.7A CN201310555042A CN103609441A CN 103609441 A CN103609441 A CN 103609441A CN 201310555042 A CN201310555042 A CN 201310555042A CN 103609441 A CN103609441 A CN 103609441A
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China
Prior art keywords
blueberry
seedling
root
medium
cultivating
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CN201310555042.7A
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CN103609441B (en
Inventor
张翠萍
王琳
李祥英
宋云
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DALIAN AISIFU AGRICULTURAL DEVELOPMENT Co Ltd
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DALIAN AISIFU AGRICULTURAL DEVELOPMENT Co Ltd
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Abstract

The invention discloses a blueberry seedling culturing method which mainly comprises the steps of: tissue culture and propagation expanding, indoor rooting, in-shed growing, and large-field seedling hardening. Through preparing two different culture mediums which are a WPM (Woody Plant Medium) normal culture medium and a hormone culture medium, a large quantity of blueberry seedlings are rapidly obtained under the condition of lower cardinal number of the seedlings, the propagation coefficient is high and the seedling culturing time consumption is reduced. By using the blueberry seedling culturing method provided by the invention, the propagation time of the blueberry seedlings is shortened by more than half a year in comparison with the same term in China; and meanwhile, due to the adoption of a plant tissue culture technology, the high purity of seedlings and the excellent root system are ensured, the plants grow very well after being planted, the fruit rate is increased by more than 10 percent in comparison with that of cut blueberry seedlings, the blueberry tissue culture rapid propagation process and the industrialized seedling production system in China can be improved, and the industrial seedling production and the commercial building of blueberry are sped up.

Description

A kind of blueberry seedling-cultivating method
Technical field
The invention belongs to the botanical seedling culturing method of agriculture field, is the seedling-cultivating method of blueberry specifically.
Background technology
Blueberry (Blueberry), has another name called cowberry, blue berry, is Ericaceae Vaccinium (Vaccinium.) plant, and perennial fallen leaves or evergreen shrubs or undershrub, be distributed widely in the Northern Hemisphere, has higher economic worth and wide DEVELOPMENT PROSPECT.Blueberry low sugar, low fat, oxidation resistance are listed as first of all fruits, vegetables, have the cerebral nerve of preventing old and feeble, strengthen cardiac function, improving eyesight and the unique effects such as anticancer, therefore by international food and agricultural organization, classified as one of the mankind's five large healthy food, will become one of the most promising fruit tree species of 21 century.Raising along with people's living standard, blueberry, raspberry, sea-buckthorn, wild jujube etc. receive people's concern day by day because having peculiar flavour and nutrition health-care functions, the ranks that are put into third generation in the world fruit, as third generation fruit, blueberry has wide market prospects, in states such as the U.S., Chile, Japan, Germany, Canada, its production enters the industrialization cultivation stage already.
Er China, because nurseries’ supplies are not enough, current area under cultivation is limited also.On the one hand traditional cuttage technique have that reproduction coefficient is low, the difficulty of taking root, breeding dimension-limited and the nursery stock of producing at aspects such as growth potential, fruit qualities the shortcoming such as poor, also cannot become the Main Means that blueberry is bred for the moment; On the other hand, although some company has adopted the blueberry tissue culture technique few with material, reproduction coefficient is high to grow seedlings, because material is limit, also exist growing-seedling period long, the problems such as seedling quality level is uneven, cause blueberry industry development slow, are difficult to meet the need of market.
Summary of the invention
Large for solving on market nursery stock demand, the problems such as production development is quick, accelerate factorial seedling growth and the commercialization construction of blueberry, and the present invention controls by hormonal readiness, temperature, illumination in regulation and control blueberry reproductive process, improve blueberry seedling production system, accelerate the blueberry process of growing seedlings.
Concrete technical scheme of the present invention is: comprise the steps:
(1) it is numerous that tissue is cultivated expansion
A. prepare medium: prepare two kinds of different medium, a kind of is the normal medium of WPM, and another kind is hormone culture-medium, in the normal medium of WPM, adds hormone;
B. inoculation: under gnotobasis, blueberry seedling is transferred in superclean bench;
C. in culturing room, cultivate: condition is that temperature is 23-27 ℃, and light application time is 16h; First in hormone culture-medium, cultivate 1-1.5 month, then be transferred in the normal medium of WPM and cultivate 1.5-2 month;
(2) indoor taking root: blueberry seedling is cut into 3-5cm segment, dip root-growing agent solution, cuttage is to taking root in matrix, and the blueberry seedling that completes the operation of taking root is placed on to temperature 22-27 ℃, in the environment of light application time 16h, be equipped with suitable water and fertilizer management 1-2 month, suitable liquid manure refers to the fertile every strain 0.3-0.8 gram of fertilizer control release, and moisture is determined and watered irrigation quantity every day according to season, weather and evaporation discharge;
(3) growth in canopy: by the good seedling of taking root, first move on in canopy and tame 1-2 month, after it adapts to external environmental condition, be transplanted in nutritive cube, be equipped with suitable water and fertilizer management, 2-3 month administrative time;
(4) land for growing field crops hardening: by stalwartness, without damage by disease and insect, root system covers with the seedling of nutritive cube and transfers in nutritious bag, be equipped with suitable water and fertilizer management, 3-4 month administrative time, suitable liquid manure refers to the fertile every strain 0.3-0.8 gram of fertilizer control release, and moisture is determined and watered irrigation quantity every day according to season, weather and evaporation discharge.
Hormone described in the present invention is zeatin, and consumption is the zeatin that every liter of WPM adds 1.5-3mg.
The solution of root-growing agent described in the present invention is ABT-1 rooting powder solution, and concentration is 0.3g/L-0.8g/L.
The matrix of taking root in the present invention is preferably broken liver moss.
Described in the present invention, nutritive cube is of a size of 10cm*10cm-12cm*12cm, and the mixture of turfy soil, perlite and garden mould is housed in nutritive cube.
Described in the present invention, nutritious bag is of a size of 15cm*15cm-25cm*25cm, and the mixture of pine bark, turfy soil and garden mould is housed in nutritious bag, has executed base fertilizer in nutritious bag simultaneously, and base fertilizer is preferably slow-release fertilizer or Controlled Release Fertilizer.
Beneficial effect of the present invention: use the blueberry of the present invention production system of growing seedlings, can make the blueberry seedling breeding time than more than shortening half a year domestic same period, due to what adopt, be plant tissue culture technique simultaneously, purity and the good root system of seedling have been guaranteed, after plant field planting, grow fine, fruiting rate improves more than 10% than cuttage blueberry seedling, can improve the fast numerous flow process of domestic blueberry tissue culture and factorial seedling growth system, accelerates factorial seedling growth and the commercialization construction of blueberry.
Embodiment
Embodiment 1:
The blueberry tissue culture seedling growing fine is transferred in the medium of WPM+2mg/LWPM Zeatin, in culturing room, cultivate after 1 month, be transferred to again in WPM medium, reproduction coefficient can reach 8, be put in culturing room and continue to cultivate 1.5 months, switching is 3 times continuously, treat that kind of seedling leaf is larger, when stem is sturdy, use ABT-1 root-growing agent to take root, be placed on Artificial Control illumination, temperature (is 23 ℃, light application time is 16h) the chamber of taking root in manage 2 months, treat that root system is about 5 centimetres, when plant grows fine, move on in domestication chamber and tame 1 month, then be transplanted in the nutritive cube in brooder, grow after 2 months, treat that root system covers with whole nutritive cube, nutritive cube is of a size of 10cm*10cm, when plant height is greater than 10 centimetres, be transplanted in the nutritious bag in hardening district, land for growing field crops, nutritious bag is of a size of 15cm*15cm, grow 3 months, can obtain sturdy, healthy and strong blueberry seedling, more than the time of breeding shortened half a year than the existing level of breeding, and seedling is healthy and strong, virus-free infection, owing to being tissue culture, root system development is good, effectively reached aforementioned goal of the invention.
Embodiment 2:
The blueberry tissue culture seedling growing fine is transferred in the medium of WPM+3mg/LWPM Zeatin, in culturing room, cultivate after 1.5 months, be transferred to again in WPM medium, reproduction coefficient can reach 6, be put in culturing room and continue to cultivate 2 months, switching is 2 times continuously, treat that kind of seedling leaf is larger, when stem is sturdy, use ABT-1 root-growing agent to take root, be placed on Artificial Control illumination, temperature (is 25 ℃, light application time is 16h) the chamber of taking root in manage 3 months, treat that root system is about 4 centimetres, when plant grows fine, move on in domestication chamber and tame 2 months, then be transplanted in the nutritive cube in brooder, grow after 3 months, treat that root system covers with whole nutritive cube, nutritive cube is of a size of 12cm*12cm, when plant height is greater than 10 centimetres, be transplanted in the nutritious bag in hardening district, land for growing field crops, nutritious bag is of a size of 20cm*20cm, grow 4 months, can obtain sturdy, healthy and strong blueberry seedling, more than the time of breeding shortened half a year than the existing level of breeding, and seedling is healthy and strong, virus-free infection, owing to being tissue culture, root system development is good, effectively reached aforementioned goal of the invention.
Embodiment 3:
The blueberry tissue culture seedling growing fine is transferred in the medium of WPM+1.5mg/LWPM Zeatin, in culturing room, cultivate after 1 month, be transferred to again in WPM medium, reproduction coefficient can reach 7, be put in culturing room and continue to cultivate 2 months, switching is 2 times continuously, treat that kind of seedling leaf is larger, when stem is sturdy, use ABT-1 root-growing agent to take root, be placed on Artificial Control illumination, temperature (is 27 ℃, light application time is 16h) the chamber of taking root in manage 3 months, treat that root system is about 5 centimetres, when plant grows fine, move on in domestication chamber and tame 2 months, then be transplanted in the nutritive cube in brooder, grow after 3 months, treat that root system covers with whole nutritive cube, nutritive cube is of a size of 12cm*12cm, when plant height is greater than 10 centimetres, be transplanted in the nutritious bag in hardening district, land for growing field crops, nutritious bag is of a size of 25cm*25cm, grow 4 months, can obtain sturdy, healthy and strong blueberry seedling, more than the time of breeding shortened half a year than the existing level of breeding, and seedling is healthy and strong, virus-free infection, owing to being tissue culture, root system development is good, effectively reached aforementioned goal of the invention.
The above; it is only preferably embodiment of the present invention; but protection scope of the present invention is not limited to this; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; according to technical scheme of the present invention and inventive concept thereof, be equal to replacement or changed, within all should being encompassed in protection scope of the present invention.

Claims (7)

1. a blueberry seedling-cultivating method, is characterized in that, comprises the steps:
(1) it is numerous that tissue is cultivated expansion:
A. prepare medium: prepare two kinds of different medium, a kind of is the normal medium of WPM, and another kind is hormone culture-medium, in the normal medium of WPM, adds hormone;
B. inoculation: under gnotobasis, blueberry seedling is transferred in superclean bench;
C. in culturing room, cultivate: condition is that temperature is 23-27 ℃, and light application time is 16h; First in hormone culture-medium, cultivate 1-1.5 month, then be transferred in the normal medium of WPM and cultivate 1.5-2 month;
(2) indoor taking root: blueberry seedling is cut into 3-5cm segment, dips root-growing agent solution, cuttage is to taking root in matrix, and the blueberry seedling that completes the operation of taking root is placed on to temperature 22-27 ℃, in the environment of light application time 16h, be equipped with water and fertilizer management 1-2 month;
(3) growth in canopy: by the good seedling of taking root, first move on in canopy and tame 1-2 month, be then transplanted in nutritive cube, be equipped with water and fertilizer management, 2-3 month administrative time;
(4) land for growing field crops hardening: by stalwartness, without damage by disease and insect, root system covers with the seedling of nutritive cube and transfers in nutritious bag, is equipped with water and fertilizer management, until seedling.
2. a kind of blueberry seedling-cultivating method according to claim 1, is characterized in that, described hormone is zeatin, and consumption is the zeatin that every liter of WPM adds 1.5-3mg.
3. a kind of blueberry seedling-cultivating method according to claim 1, is characterized in that, described root-growing agent solution is ABT-1 rooting powder solution, and concentration is 0.3g/L-0.8g/L.
4. a kind of blueberry seedling-cultivating method according to claim 1, is characterized in that, described in the matrix of taking root be broken liver moss.
5. a kind of blueberry seedling-cultivating method according to claim 1, is characterized in that, described nutritive cube is of a size of 10cm*10cm-12cm*12cm, and the mixture of turfy soil, perlite and garden mould is housed in nutritive cube.
6. a kind of blueberry seedling-cultivating method according to claim 1, is characterized in that, described nutritious bag is of a size of 15cm*15cm-25cm*25cm, and mixture and the base fertilizer of pine bark, turfy soil and garden mould is housed in nutritious bag.
7. a kind of blueberry seedling-cultivating method according to claim 6, is characterized in that, described base fertilizer is slow-release fertilizer or Controlled Release Fertilizer.
CN201310555042.7A 2013-11-11 2013-11-11 A kind of blueberry seedling-cultivating method Expired - Fee Related CN103609441B (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103798096A (en) * 2014-03-12 2014-05-21 金陵科技学院 Method for improving blueberry sapling planting quality
CN103988688A (en) * 2014-05-29 2014-08-20 太仓市泗洲农场专业合作社 High-anthocyanin-content blueberry planting method
CN104488639A (en) * 2014-11-27 2015-04-08 广德县桃州镇横山中路乐府肥羊 Cultivating method of blueberry seedling suitable for South China
CN105493944A (en) * 2015-12-14 2016-04-20 青岛沃林蓝莓果业有限公司 Blueberry seedling tending substrate and preparation method thereof
CN106358890A (en) * 2015-07-23 2017-02-01 程向东 Vaccinium uliginosum cultivation technique
CN106577094A (en) * 2016-10-27 2017-04-26 安徽紫约农业科技有限公司 Blueberry whole-matrix cultivation method
CN109089696A (en) * 2018-08-13 2018-12-28 紫云自治县天赐农林发展有限公司 A kind of blueberry method for culturing seedlings

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USPP21222P2 (en) * 2009-02-25 2010-08-24 University Of Georgia Research Foundation Southern highbush blueberry plant named ‘TH-682’
CN102475020A (en) * 2010-11-24 2012-05-30 丹东农业科学院 Northern blueberry-growing method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USPP21222P2 (en) * 2009-02-25 2010-08-24 University Of Georgia Research Foundation Southern highbush blueberry plant named ‘TH-682’
CN102475020A (en) * 2010-11-24 2012-05-30 丹东农业科学院 Northern blueberry-growing method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
龚雪元等: "蓝莓丛生芽增殖的诱导研究", 《广东农业科学》, no. 9, 10 May 2013 (2013-05-10) *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103798096A (en) * 2014-03-12 2014-05-21 金陵科技学院 Method for improving blueberry sapling planting quality
CN103988688A (en) * 2014-05-29 2014-08-20 太仓市泗洲农场专业合作社 High-anthocyanin-content blueberry planting method
CN104488639A (en) * 2014-11-27 2015-04-08 广德县桃州镇横山中路乐府肥羊 Cultivating method of blueberry seedling suitable for South China
CN106358890A (en) * 2015-07-23 2017-02-01 程向东 Vaccinium uliginosum cultivation technique
CN105493944A (en) * 2015-12-14 2016-04-20 青岛沃林蓝莓果业有限公司 Blueberry seedling tending substrate and preparation method thereof
CN106577094A (en) * 2016-10-27 2017-04-26 安徽紫约农业科技有限公司 Blueberry whole-matrix cultivation method
CN109089696A (en) * 2018-08-13 2018-12-28 紫云自治县天赐农林发展有限公司 A kind of blueberry method for culturing seedlings

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