CN107980629A - A kind of blueberry seedling fostering method - Google Patents
A kind of blueberry seedling fostering method Download PDFInfo
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- CN107980629A CN107980629A CN201610955840.2A CN201610955840A CN107980629A CN 107980629 A CN107980629 A CN 107980629A CN 201610955840 A CN201610955840 A CN 201610955840A CN 107980629 A CN107980629 A CN 107980629A
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- seedling
- blueberry
- dkw
- blueberry seedling
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of blueberry seedling fostering method, it is main comprise the step of tissue cultures expand it is numerous, indoor take root, growth and crop field hardening in canopy, by preparing two kinds of different culture mediums, one kind is DKW normal incubation mediums, one kind is hormone culture-medium so that seedling quickly obtains substantial amounts of blueberry seedling in the case where radix is relatively low, breeding coefficient is high, reduces nursery and takes.The blueberry seedling fostering method of the present invention can be such that the blueberry seedling breeding time shortens more than half a year than the domestic same period by regulating and controlling the hormonal readiness in blueberry reproductive process, temperature, illumination control；Plant tissue culture technique is used at the same time, the purity of seedling and excellent root system is ensure that, grows fine after plant field planting；Country's blueberry tissue culture numerous flow and industrialized breeding system soon are improved, accelerates industrial seedling rearing and the commercialization construction of blueberry.
Description
Technical field
The present invention relates to botanical seedling culturing method field, and in particular to and a kind of blueberry seedling fostering method.
Background technology
Blueberry is Ericaceae cowberry platymiscium, and perennial fallen leaves or evergreen shrubs or undershrub, have higher economic valency
Value and wide development prospect.Blueberry is classified as one of five big healthy food of the mankind by international food and agricultural organization.Its production already into
Enter the industrialization cultivation stage.And China due to nurseries' supplies deficiency, cultivated area is also fairly limited at present.Traditional cuttage technique
There are breeding coefficient is low, difficult, breeding dimension-limited of taking root and the nursery stock produced are poor etc. in growth potential, fruit quality etc.
Shortcoming, causes blueberry industry to develop slowly, it is difficult to meet the market demand.
The content of the invention
It is an object of the present invention to the present invention accelerates blueberry nursery process；Ensure the purity of seedling and excellent root system, plant
Grow fine after field planting；Blueberry seeling industry system is improved, accelerates industrial seedling rearing and the commercialization construction of blueberry.
Technical scheme：A kind of blueberry seedling fostering method, includes the following steps：
（1）Tissue cultures expand numerous：
A, culture medium is prepared：Two kinds of different culture mediums are prepared, one kind is DKW normal incubation mediums, and another kind is hormone culture-medium,
Add hormone i.e. in DKW normal incubation mediums；The hormone is kinetin, and dosage adds the kinetin of 45-75mg for every liter of DKW；
B, it is inoculated with：In an aseptic environment, blueberry seedling is transferred in clean work station；
C, cultivated in culturing room：Condition is that temperature is 22-26 DEG C, light application time 16h；0.5- is first cultivated in hormone culture-medium
1.5 months, then be transferred in DKW normal incubation mediums and cultivate 2-3 months；
（2）Take root interior：Blueberry seedling is cut into 4-8cm segments, dips rooting agent solution, cuttage is incited somebody to action into matrix of taking root
The blueberry seedling of operation of completing to take root is placed on 21-25 DEG C of temperature, in the environment of light application time 18h, is equipped with water and fertilizer management 1-2
Month；The rooting agent solution is heteroauxin solution, concentration 0.002g/L-0.01g/L；
（3）Growth in canopy：By good seedling of taking root, domestication 1-2 months in canopy are first moved to, is then transplanted in nutritive cube, matches somebody with somebody
With water and fertilizer management, management time 2-3 month；
（4）Crop field hardening：By healthy and strong, no disease and pests harm, the seedling that root system covers with nutritive cube is transferred in nutrient bag, is equipped with liquid manure pipe
Reason, until seedling.
In foregoing blueberry seedling fostering method, the matrix of taking root is sphagnum peat moss.
In foregoing blueberry seedling fostering method, the size of the nutrient bag is 15cm*15cm-25cm*25cm, nutrient bag
In equipped with pine bark, turfy soil, sawdust, perlite and garden mould mixture and base fertilizer.
In foregoing blueberry seedling fostering method, the base fertilizer is family's animal manure.
Compared with prior art, blueberry seedling fostering method of the invention is by regulating and controlling the hormone water in blueberry reproductive process
Flat, temperature, illumination control, can be such that the blueberry seedling breeding time shortens more than half a year than the domestic same period；Use plant tissue at the same time
Culture technique, ensure that the purity of seedling and excellent root system, grows fine after plant field planting；Improve country's blueberry tissue culture numerous stream soon
Journey and industrialized breeding system, accelerate industrial seedling rearing and the commercialization construction of blueberry.
Embodiment：
For a better understanding of the present invention, below by embodiment, the present invention is further described, and embodiment is served only for explaining this hair
It is bright, any restriction will not be formed to the present invention.
Embodiment 1：A kind of blueberry seedling fostering method, includes the following steps：
（1）Tissue cultures expand numerous：
A, culture medium is prepared：Two kinds of different culture mediums are prepared, one kind is DKW normal incubation mediums, and another kind is hormone culture-medium,
Add hormone i.e. in DKW normal incubation mediums；The hormone is kinetin, and dosage adds the kinetin of 60mg for every liter of DKW；
B, it is inoculated with：In an aseptic environment, blueberry seedling is transferred in clean work station；
C, cultivated in culturing room：Condition is that temperature is 24 DEG C, light application time 16h；First cultivated 1 month in hormone culture-medium,
It is transferred in DKW normal incubation mediums and cultivates 2-3 months again；
（2）Take root interior：Blueberry seedling is cut into 6cm segments, dips rooting agent solution, cuttage, and will be complete into matrix of taking root
Blueberry seedling into operation of taking root is placed on 23 DEG C of temperature, in the environment of light application time 18h, is equipped with water and fertilizer management 1-2 months；Institute
It is heteroauxin solution to state rooting agent solution, concentration 0.006g/L；
（3）Growth in canopy：By good seedling of taking root, domestication 1-2 months in canopy are first moved to, is then transplanted in nutritive cube, matches somebody with somebody
With water and fertilizer management, management time 2-3 month；
（4）Crop field hardening：By healthy and strong, no disease and pests harm, the seedling that root system covers with nutritive cube is transferred in nutrient bag, is equipped with liquid manure pipe
Reason, until seedling.
The matrix of taking root is sphagnum peat moss.
The size of the nutrient bag is 20cm*20cm, in nutrient bag equipped with pine bark, turfy soil, sawdust, perlite with
The mixture and base fertilizer of garden mould.
The base fertilizer is family's animal manure.
Embodiment 2：A kind of blueberry seedling fostering method, includes the following steps：
（1）Tissue cultures expand numerous：
A, culture medium is prepared：Two kinds of different culture mediums are prepared, one kind is DKW normal incubation mediums, and another kind is hormone culture-medium,
Add hormone i.e. in DKW normal incubation mediums；The hormone is kinetin, and dosage adds the kinetin of 45mg for every liter of DKW；
B, it is inoculated with：In an aseptic environment, blueberry seedling is transferred in clean work station；
C, cultivated in culturing room：Condition is that temperature is 22 DEG C, light application time 16h；0.5 is first cultivated in hormone culture-medium
Month, then be transferred in DKW normal incubation mediums and cultivate 2-3 months；
（2）Take root interior：Blueberry seedling is cut into 4cm segments, dips rooting agent solution, cuttage, and will be complete into matrix of taking root
Blueberry seedling into operation of taking root is placed on 21 DEG C of temperature, in the environment of light application time 18h, is equipped with water and fertilizer management 1-2 months；Institute
It is heteroauxin solution to state rooting agent solution, concentration 0.002g/L；
（3）Growth in canopy：By good seedling of taking root, domestication 1-2 months in canopy are first moved to, is then transplanted in nutritive cube, matches somebody with somebody
With water and fertilizer management, management time 2-3 month；
（4）Crop field hardening：By healthy and strong, no disease and pests harm, the seedling that root system covers with nutritive cube is transferred in nutrient bag, is equipped with liquid manure pipe
Reason, until seedling.
The matrix of taking root is sphagnum peat moss.
The size of the nutrient bag is 15cm*15cm, in nutrient bag equipped with pine bark, turfy soil, sawdust, perlite with
The mixture and base fertilizer of garden mould.
The base fertilizer is family's animal manure.
Embodiment 3：A kind of blueberry seedling fostering method, includes the following steps：
（1）Tissue cultures expand numerous：
A, culture medium is prepared：Two kinds of different culture mediums are prepared, one kind is DKW normal incubation mediums, and another kind is hormone culture-medium,
Add hormone i.e. in DKW normal incubation mediums；The hormone is kinetin, and dosage adds the kinetin of 75mg for every liter of DKW；
B, it is inoculated with：In an aseptic environment, blueberry seedling is transferred in clean work station；
C, cultivated in culturing room：Condition is that temperature is 26 DEG C, light application time 16h；1.5 are first cultivated in hormone culture-medium
Month, then be transferred in DKW normal incubation mediums and cultivate 2-3 months；
（2）Take root interior：Blueberry seedling is cut into 8cm segments, dips rooting agent solution, cuttage, and will be complete into matrix of taking root
Blueberry seedling into operation of taking root is placed on 25 DEG C of temperature, in the environment of light application time 18h, is equipped with water and fertilizer management 1-2 months；Institute
It is heteroauxin solution to state rooting agent solution, concentration 0.01g/L；
（3）Growth in canopy：By good seedling of taking root, domestication 1-2 months in canopy are first moved to, is then transplanted in nutritive cube, matches somebody with somebody
With water and fertilizer management, management time 2-3 month；
（4）Crop field hardening：By healthy and strong, no disease and pests harm, the seedling that root system covers with nutritive cube is transferred in nutrient bag, is equipped with liquid manure pipe
Reason, until seedling.
The matrix of taking root is sphagnum peat moss.
The size of the nutrient bag is 25cm*25cm, in nutrient bag equipped with pine bark, turfy soil, sawdust, perlite with
The mixture and base fertilizer of garden mould.
The base fertilizer is family's animal manure.
Claims (4)
- A kind of 1. blueberry seedling fostering method, it is characterised in that：Include the following steps：（1）Tissue cultures expand numerous：A, culture medium is prepared：Two kinds of different culture mediums are prepared, one kind is DKW normal incubation mediums, and another kind is hormone culture-medium, Add hormone i.e. in DKW normal incubation mediums；The hormone is kinetin, and dosage adds the kinetin of 45-75mg for every liter of DKW；B, it is inoculated with：In an aseptic environment, blueberry seedling is transferred in clean work station；C, cultivated in culturing room：Condition is that temperature is 22-26 DEG C, light application time 16h；0.5- is first cultivated in hormone culture-medium 1.5 months, then be transferred in DKW normal incubation mediums and cultivate 2-3 months；Take root interior：Blueberry seedling is cut into 4-8cm segments, dips rooting agent solution, cuttage will be completed into matrix of taking root The blueberry seedling of operation of taking root is placed on 21-25 DEG C of temperature, in the environment of light application time 18h, is equipped with water and fertilizer management 1-2 months； The rooting agent solution is heteroauxin solution, concentration 0.002g/L-0.01g/L；（3）Growth in canopy：By good seedling of taking root, domestication 1-2 months in canopy are first moved to, is then transplanted in nutritive cube, matches somebody with somebody With water and fertilizer management, management time 2-3 month；（4）Crop field hardening：By healthy and strong, no disease and pests harm, the seedling that root system covers with nutritive cube is transferred in nutrient bag, is equipped with liquid manure pipe Reason, until seedling.
- A kind of 2. blueberry seedling fostering method according to claim 1, it is characterised in that：The matrix of taking root is bog moss Bog moss.
- A kind of 3. blueberry seedling fostering method according to claim 1, it is characterised in that：The size of the nutrient bag is 15cm*15cm-25cm*25cm, mixture and bottom equipped with pine bark, turfy soil, sawdust, perlite and garden mould in nutrient bag Fertilizer.
- A kind of 4. blueberry seedling fostering method according to claim 3, it is characterised in that：The base fertilizer is family's animal manure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610955840.2A CN107980629A (en) | 2016-10-27 | 2016-10-27 | A kind of blueberry seedling fostering method |
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| CN201610955840.2A CN107980629A (en) | 2016-10-27 | 2016-10-27 | A kind of blueberry seedling fostering method |
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| CN201610955840.2A Pending CN107980629A (en) | 2016-10-27 | 2016-10-27 | A kind of blueberry seedling fostering method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109089696A (en) * | 2018-08-13 | 2018-12-28 | 紫云自治县天赐农林发展有限公司 | A kind of blueberry method for culturing seedlings |
| CN109661960A (en) * | 2018-08-24 | 2019-04-23 | 伊春林业科学院 | A kind of wild bog bilberry spray micro cuttage seedling-raising technique |
| CN110771443A (en) * | 2019-11-22 | 2020-02-11 | 阳山县三连阳生态农林开发有限公司 | Yangshan bletilla striata single culture medium formula sterile seeding seedling production process |
-
2016
- 2016-10-27 CN CN201610955840.2A patent/CN107980629A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109089696A (en) * | 2018-08-13 | 2018-12-28 | 紫云自治县天赐农林发展有限公司 | A kind of blueberry method for culturing seedlings |
| CN109661960A (en) * | 2018-08-24 | 2019-04-23 | 伊春林业科学院 | A kind of wild bog bilberry spray micro cuttage seedling-raising technique |
| CN110771443A (en) * | 2019-11-22 | 2020-02-11 | 阳山县三连阳生态农林开发有限公司 | Yangshan bletilla striata single culture medium formula sterile seeding seedling production process |
| CN110771443B (en) * | 2019-11-22 | 2021-12-21 | 阳山县三连阳生态农林开发有限公司 | Yangshan bletilla striata single culture medium formula sterile seeding seedling production process |
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Application publication date: 20180504 |