CN103609441B - A kind of blueberry seedling-cultivating method - Google Patents
A kind of blueberry seedling-cultivating method Download PDFInfo
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- CN103609441B CN103609441B CN201310555042.7A CN201310555042A CN103609441B CN 103609441 B CN103609441 B CN 103609441B CN 201310555042 A CN201310555042 A CN 201310555042A CN 103609441 B CN103609441 B CN 103609441B
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- 235000003095 Vaccinium corymbosum Nutrition 0.000 title claims abstract description 45
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- 235000021014 blueberries Nutrition 0.000 title claims abstract description 45
- 240000000851 Vaccinium corymbosum Species 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 17
- 239000002609 medium Substances 0.000 claims abstract description 18
- 239000005556 hormone Substances 0.000 claims abstract description 9
- 229940088597 hormone Drugs 0.000 claims abstract description 9
- 238000011534 incubation Methods 0.000 claims abstract description 7
- 244000037666 field crops Species 0.000 claims abstract description 6
- 239000001963 growth medium Substances 0.000 claims abstract description 5
- 230000000050 nutritive effect Effects 0.000 claims description 17
- 239000003337 fertilizer Substances 0.000 claims description 16
- 235000008935 nutritious Nutrition 0.000 claims description 13
- 238000012258 culturing Methods 0.000 claims description 9
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 claims description 7
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical group OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 229940023877 zeatin Drugs 0.000 claims description 7
- 239000011159 matrix material Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 239000002689 soil Substances 0.000 claims description 4
- 241000238631 Hexapoda Species 0.000 claims description 2
- 238000013270 controlled release Methods 0.000 claims description 2
- 201000010099 disease Diseases 0.000 claims description 2
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- 210000004185 liver Anatomy 0.000 claims description 2
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- 239000010451 perlite Substances 0.000 claims description 2
- 235000010204 pine bark Nutrition 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
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- 230000001488 breeding effect Effects 0.000 abstract description 11
- 241000196324 Embryophyta Species 0.000 abstract description 9
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Abstract
The invention discloses a kind of blueberry seedling-cultivating method, the step mainly comprised cultivate in a organized way expand numerous, take root in indoor, growth and land for growing field crops hardening in canopy, by preparing two kinds of different medium, one is WPM normal incubation medium, one is hormone culture-medium, makes seedling when radix is lower, obtains a large amount of blueberry seedlings fast, reproduction coefficient is high, reduces nursery consuming time.Use blueberry seedling-cultivating method of the present invention, the blueberry seedling breeding time can be made to shorten half a year more than than the domestic same period, simultaneously due to employing is plant tissue culture technique, ensure that the purity of seedling and excellent root system, grow fine after plant field planting, fruiting rate improves more than 10% than cuttage blueberry seedling, can improve the fast numerous flow process of domestic blueberry tissue culture and industrialized breeding system, accelerates factorial seedling growth and the commercialization construction of blueberry.
Description
Technical field
The invention belongs to the botanical seedling culturing method of agriculture field, specifically the seedling-cultivating method of blueberry.
Background technology
Blueberry (Blueberry), having another name called cowberry, blue berry, is Ericaceae Vaccinium (Vaccinium.) plant, perennial fallen leaves or evergreen shrubs or undershrub, be distributed widely in the Northern Hemisphere, there is higher economic worth and wide DEVELOPMENT PROSPECT.Blueberry low sugar, low fat, oxidation resistance arrange first of all fruits, vegetables, have prevent cerebral nerve old and feeble, strengthen cardiac function, improving eyesight and the unique effects such as anticancer, therefore be classified as one of large healthy food of the mankind five by international food and agricultural organization, will one of 21 century the most promising fruit tree species be become.Along with the raising of people's living standard, blueberry, raspberry, sea-buckthorn, wild jujube etc. are because having peculiar flavour and nutrition health-care functions and day by day receiving the concern of people, be put into the ranks of third generation in the world fruit, as third generation fruit, blueberry has wide market prospects, in states such as the U.S., Chile, Japan, Germany, Canada, its production enters the industrialization cultivation stage already.
And in China, because nurseries' supplies are not enough, current area under cultivation is also quite limited.On the one hand traditional cuttage technique have that reproduction coefficient is low, difficulty of taking root, breeding dimension-limited and the nursery stock of the producing shortcoming such as poor in growth potential, fruit quality etc., also cannot become the Main Means that blueberry is bred for the moment; On the other hand, although some company blueberry tissue culture technique that employing material is few, reproduction coefficient is high carries out nursery, because material limit, also there is growing-seedling period long, the problems such as seedling quality level is uneven, cause blueberry industry slower development, are difficult to meet the need of market.
Summary of the invention
Large for solving nursery stock demand on market, the problems such as production development is quick, accelerate factorial seedling growth and the commercialization construction of blueberry, and the present invention is controlled by the hormonal readiness in regulation and control blueberry reproductive process, temperature, illumination, improve blueberry seeling industry system, accelerate blueberry nursery process.
Concrete technical scheme of the present invention is: comprise the steps:
(1) tissue cultures expands numerous
A. medium is prepared: prepare two kinds of different medium, one is WPM normal incubation medium, and another kind is hormone culture-medium, namely in WPM normal incubation medium, adds hormone;
B. inoculate: in an aseptic environment, blueberry seedling is transferred in superclean bench;
C. cultivate in culturing room: condition is temperature is 23-27 DEG C, and light application time is 16h; In hormone culture-medium, first cultivate 1-1.5 month, then be transferred in WPM normal incubation medium and cultivate 1.5-2 month;
(2) take root in indoor: blueberry seedling is cut into 3-5cm segment, dip root-growing agent solution, cuttage is to taking root in matrix, and the blueberry seedling completing operation of taking root is placed on temperature 22-27 DEG C, in the environment of light application time 16h, be equipped with suitable water and fertilizer management 1-2 month, suitable liquid manure refers to the fertile every strain 0.3-0.8 gram of fertilizer control release, and moisture is determined to water irrigation quantity every day according to season, weather and evaporation discharge;
(3) growth in canopy: by good seedling of taking root, first moves on in canopy and tames 1-2 month, adapts to, after external environmental condition, be transplanted in nutritive cube, be equipped with suitable water and fertilizer management until it, 2-3 month administrative time;
(4) land for growing field crops hardening: by stalwartness, without damage by disease and insect, the seedling that root system covers with nutritive cube is transferred in nutritious bag, be equipped with suitable water and fertilizer management, 3-4 month administrative time, suitable liquid manure refers to the fertile every strain 0.3-0.8 gram of fertilizer control release, according to season, weather and evaporation discharge, moisture determines that every day waters irrigation quantity.
Hormone described in the present invention is zeatin, and consumption is the zeatin that often liter of WPM adds 1.5-3mg.
The solution of root-growing agent described in the present invention is ABT-1 rooting powder solution, and concentration is 0.3g/L-0.8g/L.
Matrix of taking root in the present invention is preferably broken liver moss.
Described in the present invention, nutritive cube is of a size of 10cm*10cm-12cm*12cm, and the mixture of turfy soil, perlite and garden mould is housed in nutritive cube.
Described in the present invention, nutritious bag is of a size of 15cm*15cm-25cm*25cm, and the mixture of pine bark, turfy soil and garden mould is housed in nutritious bag, and executed base fertilizer in nutritious bag, base fertilizer is preferably slow-release fertilizer or Controlled Release Fertilizer simultaneously.
Beneficial effect of the present invention: use blueberry nursery production system of the present invention, the blueberry seedling breeding time can be made to shorten half a year more than than the domestic same period, simultaneously due to employing is plant tissue culture technique, ensure that the purity of seedling and excellent root system, grow fine after plant field planting, fruiting rate improves more than 10% than cuttage blueberry seedling, can improve the fast numerous flow process of domestic blueberry tissue culture and industrialized breeding system, accelerates factorial seedling growth and the commercialization construction of blueberry.
Embodiment
Embodiment 1:
The blueberry tissue culture seedling grown fine is transferred in the medium of WPM+2mg/LWPM Zeatin, after culturing room cultivates 1 month, be transferred in WPM medium again, reproduction coefficient can reach 8, be put in culturing room and continue cultivation 1.5 months, continuous switching 3 times, treat that kind of seedling leaf is larger, when stem is sturdy, ABT-1 root-growing agent is used to take root, be placed on Artificial Control illumination, temperature (is 23 DEG C, light application time is 16h) room of taking root in management 2 months, treat that root system is about 5 centimetres, when plant grows fine, move on in domestication room and tame 1 month, then be transplanted in the nutritive cube in brooder, grow after 2 months, treat that root system covers with whole nutritive cube, nutritive cube is of a size of 10cm*10cm, when plant height is greater than 10 centimetres, be transplanted in the nutritious bag in hardening district, land for growing field crops, nutritious bag is of a size of 15cm*15cm, grow 3 months, can obtain sturdy, healthy and strong blueberry seedling, time of breeding shortens half a year more than than the existing level of breeding, and seedling is healthy and strong, virus-free infection, owing to being tissue culture, root system development is good, effectively reach aforementioned invention object.
Embodiment 2:
The blueberry tissue culture seedling grown fine is transferred in the medium of WPM+3mg/LWPM Zeatin, after culturing room cultivates 1.5 months, be transferred in WPM medium again, reproduction coefficient can reach 6, be put in culturing room and continue cultivation 2 months, continuous switching 2 times, treat that kind of seedling leaf is larger, when stem is sturdy, ABT-1 root-growing agent is used to take root, be placed on Artificial Control illumination, temperature (is 25 DEG C, light application time is 16h) room of taking root in management 3 months, treat that root system is about 4 centimetres, when plant grows fine, move on in domestication room and tame 2 months, then be transplanted in the nutritive cube in brooder, grow after 3 months, treat that root system covers with whole nutritive cube, nutritive cube is of a size of 12cm*12cm, when plant height is greater than 10 centimetres, be transplanted in the nutritious bag in hardening district, land for growing field crops, nutritious bag is of a size of 20cm*20cm, grow 4 months, can obtain sturdy, healthy and strong blueberry seedling, time of breeding shortens half a year more than than the existing level of breeding, and seedling is healthy and strong, virus-free infection, owing to being tissue culture, root system development is good, effectively reach aforementioned invention object.
Embodiment 3:
The blueberry tissue culture seedling grown fine is transferred in the medium of WPM+1.5mg/LWPM Zeatin, after culturing room cultivates 1 month, be transferred in WPM medium again, reproduction coefficient can reach 7, be put in culturing room and continue cultivation 2 months, continuous switching 2 times, treat that kind of seedling leaf is larger, when stem is sturdy, ABT-1 root-growing agent is used to take root, be placed on Artificial Control illumination, temperature (is 27 DEG C, light application time is 16h) room of taking root in management 3 months, treat that root system is about 5 centimetres, when plant grows fine, move on in domestication room and tame 2 months, then be transplanted in the nutritive cube in brooder, grow after 3 months, treat that root system covers with whole nutritive cube, nutritive cube is of a size of 12cm*12cm, when plant height is greater than 10 centimetres, be transplanted in the nutritious bag in hardening district, land for growing field crops, nutritious bag is of a size of 25cm*25cm, grow 4 months, can obtain sturdy, healthy and strong blueberry seedling, time of breeding shortens half a year more than than the existing level of breeding, and seedling is healthy and strong, virus-free infection, owing to being tissue culture, root system development is good, effectively reach aforementioned invention object.
The above; be only the present invention's preferably embodiment; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to according to technical scheme of the present invention and inventive concept thereof and replace or change, all should be encompassed within protection scope of the present invention.
Claims (5)
1. a blueberry seedling-cultivating method, is characterized in that, comprises the steps:
(1) tissue cultures expands numerous:
A. medium is prepared: prepare two kinds of different medium, one is WPM normal incubation medium, and another kind is hormone culture-medium, namely in WPM normal incubation medium, adds hormone; Described hormone is zeatin, and consumption is the zeatin that often liter of WPM adds 1.5-3mg;
B. inoculate: in an aseptic environment, blueberry seedling is transferred in superclean bench;
C. cultivate in culturing room: condition is temperature is 23-27 DEG C, and light application time is 16h; In hormone culture-medium, first cultivate 1-1.5 month, then be transferred in WPM normal incubation medium and cultivate 1.5-2 month;
(2) take root in indoor: blueberry seedling is cut into 3-5cm segment, dips root-growing agent solution, and the blueberry seedling completing operation of taking root to taking root in matrix, and is placed on temperature 22-27 DEG C by cuttage, in the environment of light application time 16h, is equipped with water and fertilizer management 1-2 month; Described root-growing agent solution is ABT-1 rooting powder solution, and concentration is 0.3g/L-0.8g/L;
(3) growth in canopy: by good seedling of taking root, first moves on in canopy and tames 1-2 month, be then transplanted in nutritive cube, be equipped with water and fertilizer management, 2-3 month administrative time;
(4) land for growing field crops hardening: by stalwartness, without damage by disease and insect, the seedling that root system covers with nutritive cube is transferred in nutritious bag, is equipped with water and fertilizer management, until seedling.
2. a kind of blueberry seedling-cultivating method according to claim 1, is characterized in that, described in matrix of taking root be broken liver moss.
3. a kind of blueberry seedling-cultivating method according to claim 1, it is characterized in that, described nutritive cube is of a size of 10cm*10cm-12cm*12cm, and the mixture of turfy soil, perlite and garden mould is housed in nutritive cube.
4. a kind of blueberry seedling-cultivating method according to claim 1, it is characterized in that, described nutritious bag is of a size of 15cm*15cm-25cm*25cm, and mixture and the base fertilizer of pine bark, turfy soil and garden mould are housed in nutritious bag.
5. a kind of blueberry seedling-cultivating method according to claim 4, is characterized in that, described base fertilizer is slow-release fertilizer or Controlled Release Fertilizer.
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| CN201310555042.7A CN103609441B (en) | 2013-11-11 | 2013-11-11 | A kind of blueberry seedling-cultivating method |
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| CN201310555042.7A CN103609441B (en) | 2013-11-11 | 2013-11-11 | A kind of blueberry seedling-cultivating method |
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| CN103609441B true CN103609441B (en) | 2015-08-12 |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103798096A (en) * | 2014-03-12 | 2014-05-21 | 金陵科技学院 | Method for improving blueberry sapling planting quality |
| CN103988688A (en) * | 2014-05-29 | 2014-08-20 | 太仓市泗洲农场专业合作社 | High-anthocyanin-content blueberry planting method |
| CN104488639A (en) * | 2014-11-27 | 2015-04-08 | 广德县桃州镇横山中路乐府肥羊 | A method for cultivating blueberry seedlings suitable for southern regions |
| CN106358890A (en) * | 2015-07-23 | 2017-02-01 | 程向东 | Vaccinium uliginosum cultivation technique |
| CN105493944B (en) * | 2015-12-14 | 2018-10-16 | 青岛沃林蓝莓果业有限公司 | A kind of blueberry seedling fosters matrix and preparation method thereof |
| CN106577094A (en) * | 2016-10-27 | 2017-04-26 | 安徽紫约农业科技有限公司 | Blueberry whole-matrix cultivation method |
| CN107258518A (en) * | 2017-08-03 | 2017-10-20 | 江苏省中国科学院植物研究所 | A kind of cultural method of promotion blueberry seedling fast-growth |
| CN109089696A (en) * | 2018-08-13 | 2018-12-28 | 紫云自治县天赐农林发展有限公司 | A kind of blueberry method for culturing seedlings |
| CN117837469A (en) * | 2024-02-22 | 2024-04-09 | 吉林农业大学 | Potted blueberry and potted blueberry nutrient soil |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| USPP21222P2 (en) * | 2009-02-25 | 2010-08-24 | University Of Georgia Research Foundation | Southern highbush blueberry plant named ‘TH-682’ |
| CN102475020B (en) * | 2010-11-24 | 2013-06-19 | 丹东农业科学院 | Northern blueberry-growing method |
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