CN102823499B - Factorized breeding method of blueberry tissue-cultured seedlings - Google Patents
Factorized breeding method of blueberry tissue-cultured seedlings Download PDFInfo
- Publication number
- CN102823499B CN102823499B CN201210349632.XA CN201210349632A CN102823499B CN 102823499 B CN102823499 B CN 102823499B CN 201210349632 A CN201210349632 A CN 201210349632A CN 102823499 B CN102823499 B CN 102823499B
- Authority
- CN
- China
- Prior art keywords
- seedling
- blueberry
- tissue culture
- blueberry tissue
- factorized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a factorized breeding method of blueberry (LM3) tissue-cultured seedlings, which is characterized by comprising the step of taking 1/4 MW culture medium as rooting medium, wherein the medium does not contain any hormone including IBA (indolebutyric acid) and NAA (naphthaleneacetic acid). Through the factorized breeding method of the blueberry tissue-cultured seedlings, the seedling rooting rate is up to 100%, and the root systems are strong and have lateral buds, the transplanting survival rate is high and the seedlings are strong and rapid in growth; and the method disclosed by the invention is short in period, low in cost and capable of completely satisfying the demand of large-area cultivation and has favorable application prospect.
Description
Technical field
The present invention relates to a kind of seedling breeding method, is the seedling industrialized mating system of a kind of blueberry tissue culture kind.
Background technology
Blueberry (Blueberry) is Ericaceae (Ericaceae) Vaccinium (Vaccinium.SPP) shrub berry fruit tree, it is current one of main fruitlet fruit trees in the world, there is very high health care, in industries such as medicine, cosmetic, food coloring and food processings, there is extensive use.Blueberry, except containing the contained organic acid of general fruit, vitamin and mineral matter, is also rich in unsaturated fatty acid, ellagic acid and various bioactivators, low sugar, low fat, and oxidation resistance shelter has first of fruit, vegetables.Thereby by international food and agricultural organization, classified as one of five large healthy food, can be rated as " king of world's fruit ", be developed in recent years integrate the most rapidly nutrition and health care the 3rd generation fruit variety, fashionable America and Europe.
China's blueberry work of introducing a fine variety starts from 20th century the mid-80, introduces a fine variety scale expanding day, seedling underproduce.More about breeding and the Study on tissue culture of blueberry kind, but relevant its research without offspring root induction is less.
Summary of the invention
In view of this, the object of the present invention is to provide the seedling industrialized mating system of a kind of blueberry tissue culture kind.
For reaching this object, the seedling industrialized mating system of blueberry tissue culture kind of the present invention, comprises the following steps:
(1) culture of rootage: when shoot proliferation Multiple Buds grows to 3～5cm when high, Multiple Buds is cut into simple bud, is forwarded in root media and carries out culture of rootage under illumination condition, obtain test-tube plantlet; Described root media is usingd 1/4MW medium as minimal medium (subordinate list 1), and adds the sucrose that concentration is 20g/L, the carragheen that concentration is 6g/L, and pH is 5.8～6.2.
(2) test-tube seedling transplanting: it is to cultivate in perlite-peat soil mixed-matrix of 3: 7 that step (1) gained test-tube seedling transplanting is entered to volume ratio, obtains blueberry tissue culture seedling.
Further, the described cultivation temperature of step (1) is 25 ± 1 ℃, and intensity of illumination is 1500～2500lux, and light application time is 12～16 hours every days;
Further, the described intensity of illumination of step (1) is 1500lux, and light application time is 12 hours every days;
Further, step (1), when shoot proliferation Multiple Buds grows to 3～5cm when high, is cut into simple bud by Multiple Buds, is forwarded to proliferated culture medium.
Beneficial effect of the present invention is: the present invention has systematically carried out the research work such as blueberry culture of rootage and test-tube seedling transplanting, sets up and optimizes the seedling industrialized mating system of blueberry tissue culture kind: bud seedling rooting rate reaches 100%, and root system is healthy and strong and have a sprouting of lateral bud; Transplanting survival rate is high, and bud seedling is healthy and strong, and growth is fast; The inventive method cycle is short, and cost is low, can meet the needs of large area large-scale planting completely, has a good application prospect.
Accompanying drawing explanation
In order to make the object, technical solutions and advantages of the present invention clearer, below in conjunction with accompanying drawing, the present invention is described in further detail, wherein:
Fig. 1 blueberry photo of taking root
Photo is bred in the batch production of Fig. 2 blueberry
Embodiment
Hereinafter with reference to accompanying drawing, the preferred embodiments of the present invention are described in detail.
It is experiment material that cucumber mountain, Yongchuan District, Chongqing City blueberry is take in the present invention, has systematically carried out the research work such as culture of rootage and test-tube seedling transplanting, sets up and has optimized the seedling industrialized mating system of blueberry tissue culture kind.
1, the optimization of root media
The bud seedling that in shoot proliferation Multiple Buds, robust growth, plant height are 3～5cm is forwarded to the root media of 5 kinds of hormon concentration proportionings, under illumination condition, carries out culture of rootage, obtain test-tube plantlet; It is minimal medium (formula of minimal medium is as shown in table 1-4) that root media be take 1/4MW, 1/2MW, 1/4CQM6,1/2CQM6 medium.
Table 1 root media is with 1/4MW
Table 2 root media 1/2MW
Table 3 root media 1/4CQM6
Table 4 root media 1/2CQM6
And add concentration be 0.1mg/LIBA and the concentration NAA that is 0.1mmg/L in contrast, medium pH is 5.8～6.2; Cultivation temperature is 25 ± 1 ℃, and intensity of illumination is 1500lux, and light application time is 12 hours every days; Observe the impact that the root media of different mineral elements and hormone concentration and media is taken root on blueberry, every kind of culture medium inoculated bud seedling number is 90,60 days take root number and bud seedling growing states of statistics afterwards.
The results are shown in Table 5.From table 52, what blueberry did not add NAA that 0.1mg/L IBA and concentration are 0.1mg/L in 1/4MW root media can induced bud seedling rooting, and while only adding the NAA that 0.1mg/LIBA and concentration are 0.1mg/L in medium, 1/4MW can not induced bud seedling rooting; Blueberry can not be taken root in 1/4CQM6,1/2CQM6 medium.What therefore, the present invention selected not add in 1/4MW root media NAA that 0.1mg/LIBA and concentration are 0.1mg/L is optimum root media.
The impact that the root media of table 5 different culture media and hormone concentration and media is taken root on blueberry
Finally explanation is, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although by with reference to the preferred embodiments of the present invention, invention has been described, but those of ordinary skill in the art is to be understood that, can to it, make various changes in the form and details, and not depart from the spirit and scope of the present invention that appended claims limits.
Claims (3)
1. the seedling industrialized mating system of blueberry tissue culture kind, is characterized in that: comprise the following steps:
(1) culture of rootage: when the shoot proliferation Multiple Buds of robust growth grows to 3～5cm when high, Multiple Buds is cut into simple bud, is forwarded in root media, carry out culture of rootage under illumination condition, obtain test-tube plantlet; Described root media code is 1/4MW, and specifically the raw material mixed preparing by following mass concentration forms, and this medium does not comprise any hormone of IBA and NAA:
(2) test-tube seedling transplanting: it is to cultivate in perlite-peat soil mixed-matrix of 3: 7 that step (1) gained test-tube seedling transplanting is entered to volume ratio, obtains blueberry tissue culture seedling.
2. the seedling industrialized mating system of blueberry tissue culture kind according to claim 1, is characterized in that: step (1) cultivation temperature is 25 ± 1 ℃, and intensity of illumination is 1500～2500lux, and light application time is 12～16 hours every days.
3. the seedling industrialized mating system of blueberry tissue culture kind according to claim 2, is characterized in that: step (1) intensity of illumination is 1500lux, and light application time is 12 hours every days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210349632.XA CN102823499B (en) | 2012-09-20 | 2012-09-20 | Factorized breeding method of blueberry tissue-cultured seedlings |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210349632.XA CN102823499B (en) | 2012-09-20 | 2012-09-20 | Factorized breeding method of blueberry tissue-cultured seedlings |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102823499A CN102823499A (en) | 2012-12-19 |
| CN102823499B true CN102823499B (en) | 2014-01-29 |
Family
ID=47326977
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210349632.XA Expired - Fee Related CN102823499B (en) | 2012-09-20 | 2012-09-20 | Factorized breeding method of blueberry tissue-cultured seedlings |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102823499B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104094815B (en) * | 2014-07-29 | 2016-03-09 | 曲靖佳沃现代农业有限公司 | A kind of blueberry tree transplanting method |
| CN106106182A (en) * | 2016-08-05 | 2016-11-16 | 四川省农业科学院经济作物育种栽培研究所 | A kind of root media cultivated for blueberry tissue culture Seedling and cultural method thereof |
| CN106172007A (en) * | 2016-08-08 | 2016-12-07 | 四川千草生物技术股份有限公司 | A kind of group training blue berry high-effective root-growing culture medium |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5177349B2 (en) * | 2007-03-12 | 2013-04-03 | 国立大学法人 宮崎大学 | Raising seedlings |
| CN101176429A (en) * | 2007-11-28 | 2008-05-14 | 浙江省农业科学院 | Culture medium composition adapting for blueberry test tube plantlet radication as well as method thereof |
| CN101699991A (en) * | 2009-10-23 | 2010-05-05 | 鲁东大学 | Method for inducing blueberries to root by using liquid culturing media and permeable matrix |
| CN102318559A (en) * | 2011-08-19 | 2012-01-18 | 温州市农业科学研究院 | Culture medium composition for rapid rooting of blueberry tissue culture seedling outside test tube and preparation method |
-
2012
- 2012-09-20 CN CN201210349632.XA patent/CN102823499B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN102823499A (en) | 2012-12-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103609441B (en) | A kind of blueberry seedling-cultivating method | |
| CN102577969B (en) | Breeding method of tissue culture seedling of lonicera macranthoides Yulei No.1 | |
| CN104885773B (en) | A kind of method of quickly breeding blue berry early stage sizing tissue culture commercial seedling | |
| CN102668959A (en) | Rapid ex vitro rooting method for blueberry tissue culture seedlings and rooting culture matrix | |
| CN101176429A (en) | Culture medium composition adapting for blueberry test tube plantlet radication as well as method thereof | |
| CN102845313A (en) | Method for quickly in-vitro actinidia kolomikta propagating | |
| CN103734009B (en) | Tissue culture method of anthurium | |
| CN103348920A (en) | Rapid propagation method for high quality seedlings of Kyara | |
| CN102210267B (en) | Method for regenerating rose into complete plant | |
| CN103461121A (en) | Ex-vitro rooting method for tissue culture seedlings of pinus massoniana | |
| CN111134011B (en) | Kiwi fruit plant fruit bearing transplanting method and fruit bearing kiwi fruit potted plant obtained by same | |
| CN102823502A (en) | Method for intermediately propagating and culturing vitis quinquangularis in vitro | |
| CN103168692B (en) | Salix saposhnikovii tissue culture method | |
| CN101874472A (en) | Tissue culture method of Pitaya | |
| CN105075777A (en) | Blueberry moss seedling breeding method | |
| CN103444536B (en) | Method for reducing tissue culture production cost of hosta plantagineu | |
| CN101548646B (en) | Method for rapidly propagating aralia elata through somatic embryo and secondary somatic embryogenesis | |
| CN102823499B (en) | Factorized breeding method of blueberry tissue-cultured seedlings | |
| CN107125137A (en) | A kind of fast breeding method of Idesia polycarpa sapling | |
| CN103039360B (en) | Method for quickly propagating leeka through tissue culture | |
| CN104335898B (en) | A kind of method of Skimmia japonica Rubella Vitro Quick Reproduction | |
| CN103416302A (en) | Method for culturing regeneration plant of somatic embryo of osmanthus fragrans Lour | |
| CN102090310A (en) | Soilless culture substrate for violet | |
| CN104686342A (en) | Asexual and rapid propagation technology for styrax tonkinensis | |
| CN102119664B (en) | Method for culturing muscari botryoides mill with plant tissues |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140129 Termination date: 20140920 |
|
| EXPY | Termination of patent right or utility model |