CN102823499B - Blueberry tissue culture seedling factory breeding method - Google Patents

Abstract

The invention discloses a factorized breeding method of blueberry (LM3) tissue-cultured seedlings, which is characterized by comprising the step of taking 1/4 MW culture medium as rooting medium, wherein the medium does not contain any hormone including IBA (indolebutyric acid) and NAA (naphthaleneacetic acid). Through the factorized breeding method of the blueberry tissue-cultured seedlings, the seedling rooting rate is up to 100%, and the root systems are strong and have lateral buds, the transplanting survival rate is high and the seedlings are strong and rapid in growth; and the method disclosed by the invention is short in period, low in cost and capable of completely satisfying the demand of large-area cultivation and has favorable application prospect.

Description

Blueberry tissue culture seedling factory breeding method

technical field

The invention relates to a seedling breeding method, which is an industrial breeding method of blueberry tissue culture seedlings.

Background technique

Blueberry (Blueberry) is a shrub berry fruit tree of Ericaceae (Ericaceae) Vaccinium.SPP. It is one of the main small fruit fruit trees in the world at present. Industries such as pigments and food processing have a wide range of uses. In addition to the organic acids, vitamins and minerals contained in ordinary fruits, blueberry fruit is also rich in unsaturated fatty acids, ellagic acid and a variety of biologically active substances. It is low in sugar and fat, and its antioxidant capacity ranks first among all fruits and vegetables. . Therefore, it is listed as one of the top five healthy foods by the International Food and Agriculture Organization, and it can be called the "King of Fruits in the World".

my country's blueberry introduction work began in the mid-1980s, and the scale of introduction has been expanding day by day, and the supply of seedlings is in short supply. There are many studies on the propagation and tissue culture of blueberry varieties, but few studies on the induced rooting of its unrooted seedlings.

Contents of the invention

In view of this, the object of the present invention is to provide a method for factory breeding of blueberry tissue culture seedlings.

To achieve this purpose, the blueberry tissue culture seedling factory breeding method of the present invention comprises the following steps:

(1) rooting culture: when the subculture multiplied clustered buds grow to a height of 3 to 5 cm, the clustered shoots were cut into single buds, transferred to the rooting medium and carried out rooting culture under light conditions to obtain test-tube plantlets; The medium uses 1/4MW medium as the basic medium (attached table 1), and adds sucrose with a concentration of 20g/L and carrageenan with a concentration of 6g/L, and the pH is 5.8-6.2.

(2) Transplanting test-tube seedlings: transplanting the test-tube seedlings obtained in step (1) into a mixed matrix of perlite-peat moss with a volume ratio of 3:7 for cultivation, to obtain blueberry tissue culture seedlings.

Further, the cultivation temperature in step (1) is 25±1°C, the light intensity is 1500-2500 lux, and the light time is 12-16 hours per day;

Further, the light intensity described in step (1) is 1500lux, and the light time is 12 hours per day;

Further, step (1) when the subcultured proliferating clustered shoots grow to a height of 3-5 cm, the clustered shoots are cut into single buds and transferred to the proliferation medium.

The beneficial effects of the present invention are: the present invention has systematically carried out research work such as blueberry rooting culture and test-tube seedling transplantation, establishes and optimizes blueberry tissue culture seedling factory breeding method: the rooting rate of sprouts reaches 100%, and the root system is strong and healthy. The side buds germinate; the transplanting survival rate is high, and the sprouts are strong and grow quickly; the method of the invention has short cycle and low cost, can fully meet the needs of large-scale cultivation, and has good application prospects.

Description of drawings

In order to make the purpose, technical solutions and advantages of the present invention clearer, the present invention will be described in further detail below in conjunction with the accompanying drawings, wherein:

Figure 1 Blueberry Rooting Photo

Figure 2 Photos of blueberry factory breeding

Detailed ways

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

The invention takes the Cuguashan blueberry in Yongchuan District, Chongqing City as the experimental material, systematically carries out research work such as rooting culture and test-tube seedling transplantation, and establishes and optimizes the blueberry tissue culture seedling factory breeding method.

1. Optimization of rooting medium

The sprouts growing vigorously and with a plant height of 3-5 cm from the subcultured proliferating clustered buds were transferred to five rooting media with different hormone concentration ratios, and the rooting culture was carried out under light conditions to obtain test-tube plantlets; the rooting media was 1/4MW, 1/2MW, 1/4CQM6, and 1/2CQM6 medium are basic medium (the formula of basic medium is shown in Table 1-4).

Table 1 rooting medium with 1/4MW

Table 2 rooting medium 1/2MW

Table 3 rooting medium 1/4CQM6

Table 4 rooting medium 1/2CQM6

And add the concentration of 0.1mg/LIBA and the concentration of 0.1mmg/L of NAA as a control, the pH of the medium is 5.8-6.2; the culture temperature is 25±1°C, the light intensity is 1500lux, and the light time is 12 hours a day; observe different The effect of the rooting medium with the concentration ratio of mineral elements and hormones on blueberry rooting. The number of sprouts inoculated with each medium was 90, and the number of roots and the growth of sprouts were counted after 60 days.

The results are shown in Table 5. It can be seen from Table 52 that blueberries can induce rooting of sprouts without adding 0.1mg/L IBA and NAA with a concentration of 0.1mg/L in the 1/4MW rooting medium. When only 0.1mg/LIBA is added to the medium and the concentration is When 0.1mg/L NAA was used, 1/4MW could not induce sprouts to take root; blueberry could not take root in 1/4CQM6, 1/2CQM6 medium. Therefore, the present invention selects 1/4MW rooting medium without adding 0.1mg/LIBA and NAA with a concentration of 0.1mg/L as the optimal rooting medium.

Table 5 Effects of rooting media with different media and hormone concentration ratios on blueberry rooting

Finally, it is noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described with reference to the preferred embodiments of the present invention, those skilled in the art should understand that it can be described in the form Various changes may be made in matter and details thereof without departing from the spirit and scope of the invention as defined in the appended claims.

Claims (3)

1. the seedling industrialized mating system of blueberry tissue culture kind, is characterized in that: comprise the following steps:

(1) culture of rootage: when the shoot proliferation Multiple Buds of robust growth grows to 3～5cm when high, Multiple Buds is cut into simple bud, is forwarded in root media, carry out culture of rootage under illumination condition, obtain test-tube plantlet; Described root media code is 1/4MW, and specifically the raw material mixed preparing by following mass concentration forms, and this medium does not comprise any hormone of IBA and NAA:

(2) test-tube seedling transplanting: it is to cultivate in perlite-peat soil mixed-matrix of 3: 7 that step (1) gained test-tube seedling transplanting is entered to volume ratio, obtains blueberry tissue culture seedling.

2. the seedling industrialized mating system of blueberry tissue culture kind according to claim 1, is characterized in that: step (1) cultivation temperature is 25 ± 1 ℃, and intensity of illumination is 1500～2500lux, and light application time is 12～16 hours every days.

3. the seedling industrialized mating system of blueberry tissue culture kind according to claim 2, is characterized in that: step (1) intensity of illumination is 1500lux, and light application time is 12 hours every days.

Images