CN111134011B - Kiwi fruit plant fruit bearing transplanting method and fruit bearing kiwi fruit potted plant obtained by same - Google Patents
Kiwi fruit plant fruit bearing transplanting method and fruit bearing kiwi fruit potted plant obtained by same Download PDFInfo
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- CN111134011B CN111134011B CN202010016048.7A CN202010016048A CN111134011B CN 111134011 B CN111134011 B CN 111134011B CN 202010016048 A CN202010016048 A CN 202010016048A CN 111134011 B CN111134011 B CN 111134011B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G23/00—Forestry
- A01G23/02—Transplanting, uprooting, felling or delimbing trees
- A01G23/04—Transplanting trees; Devices for grasping the root ball, e.g. stump forceps; Wrappings or packages for transporting trees
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The invention relates to a kiwi fruit plant fruit-bearing transplanting method and a kiwi fruit pot plant with fruit obtained by the method, which comprises the following steps: selecting fruiting mother branches with the diameter of 5-10 mm in the leaf spreading period, and performing girdling treatment at the position 3.0-5.0 cm below full bud marks, wherein the girdling width is about 1.0-1.5 cm; firstly, coating IBA solution with the concentration of 100 mg/L-1000 mg/L on a girdling part, and then coating a mixed matrix containing PGPR on the girdling part; inducing and rooting: supplementing 50ml of water into the mixed matrix every 2-3 days, supplementing 40-60ml of IBA solution with the concentration of 80-100 mg/L into the mixed matrix every 5-9 days, rooting for 1-2 months, injecting 1/2MS +0.5mg/L of IBA liquid culture medium into the mixed matrix every 2-3 days through a special infusion bag for big trees, and strengthening the root system of branches for 1-2 months; and cutting the branches from the tree after the root systems of the branches become strong, and separately cultivating to obtain the kiwi fruit plants with fruits. The method solves the problems of long obtaining time and low survival rate of the potted kiwi fruit with fruit in the prior art.
Description
Technical Field
The invention belongs to the field of seedling propagation, and particularly relates to a fruit-bearing transplanting method for kiwi fruit plants and a fruit-bearing kiwi fruit potted plant obtained by the same.
Background
Kiwi fruit is a large deciduous woody vine of male and female heterozygotic plants, is known as the king of fruit, has rich nutritive value, and is rich in nutrients such as vitamins, mineral substances, amino acids, carotenoid and the like. Moreover, the kiwi fruit vines are wound and bent, have dense branches and leaves and overflow flower fragrance, can be used for vertical greening of flower stands, wall walls and the like, can also be used as household potted plants and the like, and has high ornamental value.
The breeding method of the kiwi fruits at home and abroad mainly comprises a seedling breeding method, a grafting breeding method, a plant division breeding method, a cutting breeding method and a high-altitude layering breeding method. The seedling has a long juvenile period, the characteristics of the male and female variant plants cause the separation of the characters of the offspring, the difference between the fruit quality and the parent is large, and the commercial planting benefit is delayed and unstable. Grafting is a main means for breeding kiwi fruits, but the fruiting period of grafted seedlings usually needs more than 3 years. The cuttage and high-altitude (high-branch) layering propagation can realize the large seedling planting, and the period from the planting to the benefit is shortened. Many experimental researches have been carried out on kiwi fruit cuttage and high-altitude layering propagation, but the application of cuttage and high-altitude layering in production is restricted by the problems of undeveloped root systems, few roots, few lateral roots, low transplanting and planting survival rate and the like, and in the current domestic and foreign emerging home gardening activities of fruit tree potting, the kiwi fruit potting is mostly produced by cuttage or seedling planting so as to obtain a fruiting kiwi fruit potting, and the kiwi fruit potting needs 3 years of long waiting to obtain the kiwi fruit potting.
Disclosure of Invention
The technical problem solved by the invention is as follows: the method is used for solving the problems of long obtaining time and low survival rate of fruiting potted plants in the prior art.
The invention provides a fruit bearing transplanting method for kiwi fruit plants, which comprises the following specific solutions:
1) branch treatment: selecting fruiting mother branches with the diameter of 5-10 mm in the leaf spreading period, and performing girdling treatment at the position 3.0-5.0 cm below full bud marks, wherein the girdling width is about 1.0-1.5 cm;
2) wrapping branches: firstly, coating IBA solution with the concentration of 100 mg/L-1000 mg/L on a girdling part, and then coating a mixed matrix containing PGPR on the girdling part;
3) inducing and rooting: supplementing 50ml of water into the mixed matrix every 2-3 days, supplementing 40-60ml of IBA solution with the concentration of 80-100 mg/L into the mixed matrix every 5-9 days, rooting for 1-2 months, injecting 1/2MS +0.5mg/L of IBA liquid culture medium into the mixed matrix every 2-3 days through a special infusion bag for big trees, and strengthening the root system of branches for 1-2 months;
4) transplanting: and cutting the branches from the tree after the root systems of the branches become strong, and separately cultivating to obtain the kiwi fruit plants with fruits.
Based on the method, the fruiting mother branches in the leaf-expanding period are subjected to girdling wound treatment and branch wrapping, and the girdled parts on the fruiting mother branches are treated by some rooting measures and rooting agents to stimulate the fruiting mother branches to generate callus and induce differentiation and rooting, and after the root systems are strong, the mother bodies are cut off and transplanted into a substrate for culture, so that the kiwi fruit potted plants with fruits can be quickly obtained and sold as commodities. Based on the method, the fruit can also grow normally in the process of inducing the shoot to root for 2-4 months, the kiwi fruit plant with the fruit can be harvested after the root system of the shoot is strengthened and cut off from the tree and transplanted and fixed, the potted fruit time is greatly shortened, and the survival rate of the plant is high.
On the basis of the scheme, the invention can be further improved as follows:
further, the fruiting mother branches selected in the step 1) are biennial fruiting mother branches.
Under the condition, the method is favorable for the differentiation and rooting of the branches, the survival rate of the kiwi fruit with fruit is high, and the fruit is healthy.
Specifically, before the girdling treatment is carried out on the fruit mother branches, the girdling tools and the hands of operators are disinfected by 65-85% alcohol.
Further, when the concentration of the IBA solution in the step 2) is 80-120mg/L, the IBA solution is directly smeared on the girdling part, and when the concentration of the IBA solution is 800-1200 mg/L, the IBA solution is smeared on the girdling part for 30s and then washed and removed.
The continuous stimulation of the wound part by the low-concentration IBA solution or the transient stimulation of the wound part by the high-concentration IBA solution is beneficial to inducing the growth of the callus.
Specifically, the IBA solution is prepared by dissolving IBA powder with 95% ethanol, and diluting with distilled water to desired concentration.
Further, wrapping the girdling part with a mixed matrix which is 8-10 cm long and 6-8 cm in diameter and contains 3-8 g of PGPR in the step 2).
Plant rhizosphere growth promoting bacteria (PGPR) is a general term for beneficial bacteria which have the functions of promoting plant growth or antagonizing pathogenic bacteria, is used for creating soil environment beneficial to rooting and has the function of promoting rooting induction of kiwi branches and trunks.
Specifically, the PGPR is derived from a commercially available PGPR microbial functional package.
Further, when wrapping is carried out in the step 2), wrapping and tightening by using a film, and arranging a plurality of small holes at two ends of the film.
Therefore, the arrangement of the small holes achieves the effects of ventilation and water tightness, the girdling part is convenient to ventilate, and nutrient solution and water are supplemented.
Further, in step 3), the mixing matrix is supplemented with water and IBA solution by means of a syringe.
Thus, the continuous supply of water and IBA solution can maintain the appropriate moisture content of the wrapped part and can cause the callus or root to be continuously stimulated, thereby promoting the growth of the callus or root.
Further, the step 5) comprises the following steps:
51) cutting off the branches from the trees, and transplanting the branches into a pot culture, wherein the matrix in the pot culture comprises peat soil, perlite and vermiculite, and the volume ratio of the peat soil, the perlite and the vermiculite in the matrix is 2: 0.5: 1.
52) culturing for 2-4 weeks in an environment with the temperature of 18-22 ℃ and the relative humidity of 85-95%, and then culturing in an environment with the temperature of 23-27 ℃ and the relative humidity of 70-80% to obtain the kiwi fruit plants with fruits.
Further, the mixed matrix in the step 2) comprises peat soil, perlite and vermiculite, and the volume ratio of the peat soil to the perlite to the vermiculite is 2: 1: 1.
specifically, in the step 3), the liquid culture medium of 1/2MS +0.05mg/L IBA can provide sufficient mineral nutrition and moisture for the induced new roots and promote the growth of root systems, and the liquid culture medium of 1/2MS +0.5mg/L IBA has the following formula:
based on the method, the time limit for obtaining the fruit-bearing Chinese gooseberry potted plant is shortened to about six months from the original three years, and the time for obtaining the fruit-bearing Chinese gooseberry potted plant is greatly shortened. The method can obtain the kiwi fruit potted plant which can bear fruits in the same year in a short time for appreciation or basic research, for example, the experiments such as instantaneous genetic transformation of fruits and the influence of different cultivation conditions on the fruit development can be carried out under indoor controllable conditions.
The invention also provides a potted kiwi fruit with fruit obtained by the method, which can be sold as an ornamental commodity.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Detailed Description
The following detailed description of embodiments of the invention is intended to be illustrative, and is not to be construed as limiting the invention.
Example 1
The specific solution provided by the invention comprises the following steps:
(1) the ring-peeling tool and the operator's hands were sterilized with 75% alcohol.
(2) Taking a variety of the Kiwi berry as an object, selecting healthy biennial fruiting mother branches with the diameter of 7mm in the leaf expanding period in the clear morning in the april in south, and performing girdling treatment at a position 4cm below a full bud mark, wherein the girdling width is 1.25 cm.
(3) Dissolving IBA powder with 95% ethanol, and preparing 100mg/LIBA solution with distilled water, and directly applying on girdling part.
(4) Mixing peat soil, perlite and vermiculite according to a volume ratio of 2: 1: 1, mixing to obtain a mixed matrix, then wrapping the mixed matrix which is 9cm long and 7cm in diameter and contains 5g of PGPR at a girdling position, finally wrapping and tightening by using a black film, and respectively poking 6 small holes at two ends of the film so as to facilitate ventilation and supplement nutrient solution and moisture.
(5) And (2) supplementing 50ml of tap water into the black film by using an injector every 2 days, injecting 50ml of IBA solution with the concentration of 100mg/L into the position of the wrapping matrix by using the injector every 7 days to stimulate the rooting of the black film, after the rooting is carried out for 1 more months, adding 1000ml of 1/2MS +0.5mg/L of IBA liquid culture medium into the special infusion bag for the big trees, starting a switch every 2 days to inject nutrients into the matrix, and after 1 more months, strengthening the root system of the branch.
(6) Cutting off the branches from the trees, transplanting the branches into a pot culture for growing, wherein the matrix in the pot culture is prepared from peat soil, perlite and vermiculite according to the volume ratio of 2: 0.5: 1 are mixed to obtain the product.
(7) The transplanted kiwi fruit is firstly cultured for 3 weeks in an environment with the temperature of 20 ℃ and the relative humidity of 90%, and then is cultured in an environment with the temperature of 25 ℃ and the relative humidity of 75% to obtain the potted kiwi fruit with fruit.
Observing the potted plant condition of the transplanted fruit-bearing kiwi fruit, and displaying the result: the transplanted kiwi fruit carrying plants can grow normally at normal temperature, abnormal symptoms do not appear on roots, leaves and fruits, and the kiwi fruit carrying transplanting success is proved, and the survival rate of the kiwi fruit carrying plants in the same batch is 73%.
Example 2
The specific solution provided by the invention comprises the following steps:
(1) the ring-peeling tool and the operator's hands were sterilized with 75% alcohol.
(2) Taking a variety of the Kiwi berry as an object, selecting healthy biennial fruiting mother branches with the diameter of 10.04mm in the leaf expanding period in the clear morning in the april in south, and performing girdling treatment at a position 4cm below a full bud mark, wherein the girdling width is 1 cm.
(3) Dissolving IBA powder with 95% ethanol, preparing 1000mg/LIBA solution with distilled water, smearing on the girdling part, and washing with clear water after 30 s.
(4) Mixing peat soil, perlite and vermiculite according to a volume ratio of 2: 1: 1, mixing to obtain a mixed matrix, then wrapping the mixed matrix which is 9cm long and 7cm in diameter and contains 5g of PGPR at a girdling position, finally wrapping and tightening by using a black film, and respectively poking 6 small holes at two ends of the film so as to facilitate ventilation and supplement nutrient solution and moisture.
(5) Supplementing 50ml of tap water into the black film by using an injector every 3 days, injecting 50ml of IBA solution with the concentration of 100mg/L into the position of the wrapping matrix by using the injector every 7 days to stimulate the rooting of the black film, adding 1000ml of 1/2MS +0.5mg/L of IBA liquid culture medium into a special infusion bag for the big trees after the rooting of the big trees for 1 month, starting a switch to inject nutrients into the matrix every 3 days, and strengthening the root system of the branch after 1 month,
(6) cutting off the branches from the trees, transplanting the branches into a pot culture for growing, wherein the matrix in the pot culture is prepared from peat soil, perlite and vermiculite according to the volume ratio of 2: 0.5: 1 are mixed to obtain the product.
(7) The transplanted kiwi fruit is firstly cultured for 3 weeks in an environment with the temperature of 20 ℃ and the relative humidity of 90%, and then is cultured in an environment with the temperature of 25 ℃ and the relative humidity of 75% to obtain the potted kiwi fruit with fruit.
Observing the potted plant condition of the transplanted fruit-bearing kiwi fruit, and displaying the result: the transplanted kiwi fruit carrying plants can grow normally under the normal temperature condition, no abnormal symptoms appear on roots, leaves and fruits, the kiwi fruit carrying transplanting success is shown, and the survival rate of the plants in the same batch is 65%.
Comparative example 1
The method comprises the following steps:
(1) the ring-peeling tool and the operator's hands were sterilized with 75% alcohol.
(2) Selecting healthy biennial fruiting mother branches with the diameter of 7mm in the leaf expanding period in the clear morning in the april south in sunny weather, scraping 4cm below full bud marks by using a knife to remove the epidermis of branches and trunks and expose green phloem, wherein the width of the knife is 1 cm.
(3) Dissolving IBA powder with 95% ethanol, and preparing 100mg/LIBA solution with distilled water, and directly applying on girdling part.
(4) Mixing peat soil, perlite and vermiculite according to a volume ratio of 2: 1: 1, mixing to obtain a mixed matrix, then wrapping the mixed matrix which is 9cm long and 7cm in diameter and contains 5g of PGPR at a girdling position, finally wrapping and tightening by using a black film, and respectively poking 6 small holes at two ends of the film so as to facilitate ventilation and supplement nutrient solution and moisture.
(5) And (2) supplementing 50ml of tap water into the black film by using an injector every 2 days, injecting 50ml of IBA solution with the concentration of 100mg/L into the position wrapping the matrix by using the injector every 7 days to stimulate the rooting of the black film, after the rooting is carried out for 1 more months, adding 1000ml of 1/2MS +0.5mg/LIBA liquid culture medium into the special infusion bag for the big tree, starting a switch every 2 days to inject nutrients into the matrix, and after 1 more months, the branches are unchanged and no callus is generated.
(6) Cutting off the branches from the trees, transplanting the branches into a pot culture for growing, wherein the matrix in the pot culture is prepared from peat soil, perlite and vermiculite according to the volume ratio of 2: 0.5: 1 are mixed to obtain the product.
(7) The transplanted kiwi fruits are firstly cultured for 3 weeks in an environment with the temperature of 20 ℃ and the relative humidity of 90 percent.
Compared with the embodiment 1, the comparative example 1 changes the ring peeling treatment into knife scraping, the potted condition of the transplanted fruit-bearing kiwi fruit is observed, and the result shows that: the transplanted kiwi fruit branches do not root and cannot absorb water in the matrix, so that the kiwi fruit branches wither due to excessive water loss, and the instruction girdling treatment plays an important role in inducing the kiwi fruit to generate callus and root.
Comparative example 2
The method comprises the following steps:
(1) the ring-peeling tool and the operator's hands were sterilized with 75% alcohol.
(2) Taking a variety of the Kiwi berry as an object, selecting strong-growing and healthy biennial fruiting mother branches with the diameter of 9.98mm in the clear morning in the april in south, and performing girdling treatment at a position 4cm below a full bud mark, wherein the girdling width is 1 cm.
(3) Dissolving IBA powder with 95% ethanol, preparing 1000mg/LIBA solution with distilled water, smearing on the girdling part, and washing with clear water after 30 s.
(4) Mixing peat soil, perlite and vermiculite according to a volume ratio of 2: 1: 1, mixing to obtain a mixed matrix, then wrapping the mixed matrix which is 9cm long and 7cm in diameter and contains 5g of PGPR at a girdling position, finally wrapping and tightening by using a black film, and respectively poking 6 small holes at two ends of the film so as to facilitate ventilation and supplement nutrient solution and moisture.
(5) Every 3 days, 50ml of tap water was added to the black film by syringe, and after 2 more months, callus was generated but no root was taken.
(6) Cutting off the branches from the trees, transplanting the branches into a pot culture for growing, wherein the matrix in the pot culture is prepared from peat soil, perlite and vermiculite according to the volume ratio of 2: 0.5: 1 are mixed to obtain the product.
(7) The transplanted kiwi fruits are firstly cultured for 3 weeks in an environment with the temperature of 20 ℃ and the relative humidity of 90 percent.
Compared with the example 2, the comparative example 2 does not inject IBA solution and nutrient solution regularly, the potted condition of the transplanted fruit-bearing kiwi fruit is observed, and the result shows that: the transplanted kiwi fruit branches are not rooted and cannot absorb water in the matrix, so that the kiwi fruit branches die due to excessive water loss, and the fact that the kiwi fruit girdling part is stimulated by regularly using IBA solution and 1/2MS +0.5mg/L IBA liquid culture medium is important for inducing the kiwi fruit to generate callus and take root is shown.
Based on the method, the fruit bearing mother branches in the leaf expanding period are subjected to girdling wound treatment and branch wrapping, the girdled parts on the fruit bearing mother branches are treated by using a plurality of rooting measures and rooting agents, the generation of callus is stimulated, differentiation and rooting are induced, after the root system is strong, the mother bodies are cut off and transplanted into a substrate for culture, the kiwi fruit pot with fruits can be quickly obtained, and the survival rate of plants can reach 73%.
Although embodiments of the present invention have been described in detail above, those of ordinary skill in the art will understand that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.
Claims (4)
1. A kiwi fruit plant fruit bearing transplanting method is characterized by comprising the following steps:
1) branch treatment: selecting biennial fruiting mother branches at the leaf spreading stage and with the diameter of 5-10 mm, and performing girdling treatment at the position 3.0-5.0 cm below full bud marks, wherein the girdling width is 1.0-1.5 cm;
2) wrapping branches: firstly, coating an IBA solution with the concentration of 100-1000 mg/L on a girdling part, directly coating the IBA solution on the girdling part when the concentration of the IBA solution is 80-120mg/L, coating the IBA solution on the girdling part for 30s and then washing and removing the IBA solution when the concentration of the IBA solution is 800-1200 mg/L, then coating a mixed matrix which is 8-10 cm long and 6-8 cm in diameter and contains 3-8 g of PGPR on the girdling part, wrapping and tightening the mixed matrix by using a film, and arranging a plurality of small holes at two ends of the film;
3) inducing and rooting: supplementing 50ml of water into the mixed matrix every 2-3 days, supplementing 40-60ml of IBA solution with the concentration of 80-100 mg/L into the mixed matrix every 5-9 days, rooting for 1-2 months, injecting 1/2MS +0.5mg/L of IBA liquid culture medium into the mixed matrix every 2-3 days through a special infusion bag for big trees, and strengthening the root system of branches for 1-2 months;
4) transplanting: and cutting the branches from the tree after the root systems of the branches become strong, and separately cultivating to obtain the kiwi fruit plants with fruits.
2. The fruit bearing transplantation method of kiwi plant of claim 1, wherein in step 3), water and IBA solution are added to said mixed matrix by syringe.
3. The fruit bearing transplanting method of kiwi plant according to any one of claims 1-2, wherein said step 4) comprises the steps of:
41) cutting off the branches from the trees, and transplanting the branches into a pot culture, wherein the matrix in the pot culture comprises peat soil, perlite and vermiculite, and the volume ratio of the peat soil, the perlite and the vermiculite in the matrix is 2: 0.5: 1;
42) culturing for 2-4 weeks in an environment with the temperature of 18-22 ℃ and the relative humidity of 85-95%, and then culturing in an environment with the temperature of 23-27 ℃ and the relative humidity of 70-80% to obtain the kiwi fruit plants with fruits.
4. The fruit-bearing transplanting method of kiwi fruit plants according to claim 1, wherein the mixed matrix in step 2) comprises peat soil, perlite and vermiculite, and the volume ratio of peat soil, perlite and vermiculite is 2: 1: 1.
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CN113796249A (en) * | 2021-08-09 | 2021-12-17 | 周林 | Efficient cultivation method for cultivating kiwi bonsai landscape fruits by mother plant |
CN115735661A (en) * | 2022-11-25 | 2023-03-07 | 北京市农林科学院 | Propagation method for fruit tree green branch rooting |
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