CN112470730A - Asexual cuttage propagation method for actinidia arguta - Google Patents
Asexual cuttage propagation method for actinidia arguta Download PDFInfo
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- CN112470730A CN112470730A CN202011431401.4A CN202011431401A CN112470730A CN 112470730 A CN112470730 A CN 112470730A CN 202011431401 A CN202011431401 A CN 202011431401A CN 112470730 A CN112470730 A CN 112470730A
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- China
- Prior art keywords
- cutting
- cuttage
- branches
- actinidia arguta
- asexual
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Links
- 244000298800 Actinidia arguta Species 0.000 title claims abstract description 28
- 235000016416 Actinidia arguta Nutrition 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000005520 cutting process Methods 0.000 claims abstract description 46
- 239000002689 soil Substances 0.000 claims abstract description 8
- 239000010455 vermiculite Substances 0.000 claims abstract description 8
- 229910052902 vermiculite Inorganic materials 0.000 claims abstract description 8
- 235000019354 vermiculite Nutrition 0.000 claims abstract description 8
- 239000003864 humus Substances 0.000 claims abstract description 7
- 239000000843 powder Substances 0.000 claims abstract description 7
- 239000002985 plastic film Substances 0.000 claims abstract description 4
- 229920006255 plastic film Polymers 0.000 claims abstract description 4
- 238000007789 sealing Methods 0.000 claims abstract description 4
- 238000002791 soaking Methods 0.000 claims abstract description 4
- 239000000758 substrate Substances 0.000 claims abstract description 4
- 201000010099 disease Diseases 0.000 claims abstract description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 3
- 239000004744 fabric Substances 0.000 claims abstract description 3
- 239000003630 growth substance Substances 0.000 claims description 6
- 239000002023 wood Substances 0.000 claims description 5
- 238000004080 punching Methods 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 2
- 238000010790 dilution Methods 0.000 claims description 2
- 239000000243 solution Substances 0.000 claims description 2
- 239000012173 sealing wax Substances 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 241000219066 Actinidiaceae Species 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 241000219068 Actinidia Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000014639 sexual reproduction Effects 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Developmental Biology & Embryology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a actinidia arguta asexual cutting propagation method, which comprises the following steps: collecting branches in 10-1 month, and selecting annual growing branches which are strong in growth, free of disease spots and full in lateral buds; preparing a substrate from humus soil and vermiculite exposed to the sun; wrapping the collected branches with wet cloth, placing in a 4-degree refrigerator for 10-15 days, taking out, cutting into 20-25cm cutting slips containing 3-5 robust axillary buds, sealing wax at the top ends of the cutting slips or sealing with a plastic film; and soaking the base part of the cutting slip in the rooting powder solution overnight, and compacting after cutting. The method solves the problems of low cuttage rooting rate and low rooting quantity of actinidia arguta, shortens the propagation period, and enables cuttage seedling raising and field cultivation to be efficiently connected.
Description
Technical Field
The invention belongs to the technical field of plant vegetative propagation, and particularly relates to a vegetative cutting propagation method of actinidia arguta.
Background
Actinidia arguta (Actinidia arguta) belongs to Actinidiaceae (Actinidiaceae) Actinidia, and Escholzia speciosa. The actinidia arguta grows fast, the actinidia arguta is early in fruiting, the yield is high, the cutting seedlings enter the full bearing period in the third year after the field is fixed, the full bearing period can reach 20-30 years, and the yield per mu in the full bearing period can reach 1750 kg. The actinidia arguta is rich in sugar, vitamins and minerals, can be directly eaten when being mature, and is an important emerging fruit in the world fruit market.
Sexual reproduction can not keep the excellent characteristics of each variety of actinidia arguta, an effective way to overcome the problem is asexual propagation, and compared with tissue culture, cuttage is a propagation method with high efficiency and low requirements on production equipment.
The branch cuttage is suitable for the mass amplification propagation of actinidia arguta, the callus can be quickly formed at the base of the cuttage shoot, and a large amount of adventitious roots can be formed on the callus. The cutting seedlings grow rapidly and vigorously after being planted in the field.
Disclosure of Invention
The invention aims to provide a actinidia arguta asexual cuttage propagation method, so that the problem that cuttage is difficult to root is solved, and a rapid and effective actinidia arguta asexual propagation path is created.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention specifically provides a actinidia arguta asexual cuttage propagation method, which comprises the following steps:
cutting time
The collection time of the branches is 10-1 month, and annual growing branches which are strong in growth, free of disease spots and full in lateral buds are selected;
② seedbed preparation and cuttage environment
Preparing a substrate from humus soil and vermiculite exposed to the sun;
thirdly, selection of cutting slips and treatment of cutting branches
Firstly, wrapping collected branches in a 4-degree refrigerator by using wet cloth and placing for 10-15 days; then taking out the branches, cutting the branches into 20-25cm cuttings containing 3-5 robust axillary buds, and sealing the tops of the cuttings with wax or plastic films;
pretreatment of growth regulator
Soaking the base part of the cutting in a rooting powder solution overnight;
fifthly, cutting
Firstly, punching with a wood stick with the same thickness, and compacting after cuttage, wherein the cuttage depth is 8-10 cm;
management after cuttage
After cuttage, the temperature in the greenhouse is controlled to be 20-25 ℃, and the relative humidity of the environment is controlled to be 80% -90%.
Preferably, the humus soil and vermiculite in the second step are prepared according to the volume ratio of 9: 1.
Preferably, the dilution multiple of the rooting powder in the step (iv) is 500 times.
The invention has the following advantages and positive effects:
firstly, the invention selects annual lignified branches and common growth regulators for cuttage propagation, promotes the quick and massive rooting of the cuttings, and the branches and leaves grow vigorously, thus being one of effective ways for quick propagation of fine actinidia arguta varieties;
secondly, the operation method is simple and efficient, and complex experimental equipment and expensive reagents are not needed; the cost is low, the purchase is easy, the large-scale cutting propagation can be carried out, and the effect is obvious;
the method solves the problems of low rooting rate and low rooting amount of actinidia arguta in cuttage, shortens the propagation period, and enables cuttage seedling raising and field cultivation to be efficiently connected.
In a word, the actinidia arguta breeding method provided by the invention is simple, feasible and efficient, can be used for rapidly breeding a large number of excellent actinidia arguta varieties, reduces the cost of large-scale popularization of actinidia arguta, and provides high-quality nursery stocks for wide fruit growers to plant actinidia arguta.
Detailed Description
The following embodiments are combined to further illustrate the asexual cutting propagation method of actinidia arguta of the present invention:
1. time for picking up strips
The time for picking the branches is selected from 10 months to 1 month, at the moment, the buds are full, and the branches accumulate rich nutrition.
2. Cutting bed preparation and cutting environment
Preparing a matrix from humus soil and vermiculite according to a volume of 9:1 after the humus soil and the vermiculite are exposed to the sun for one week, wherein the matrix is formed by mixing the vermiculite and organic soil, the air permeability and nutrition are guaranteed, the thickness of the seedbed matrix is 40cm, and the seedbed matrix is placed in a shady place provided with rotary spraying and heating;
3. selection of cutting slips and treatment of cutting branches
The branches which are placed in a 4-degree refrigerator for 10-15 days are taken out and cut into cutting slips of 20-25cm, the cutting slips comprise 3-5 robust axillary buds, and the tops of the cutting slips are sealed by melted wax (or plastic films).
4. Pretreatment with rooting or growth regulators
The cuttings are soaked in 3721 rooting powder diluted by 500 times for one night.
5. Cutting
When in cuttage, a wood stick with the same thickness is used for punching, the wood stick is compacted after cuttage, and the cuttage depth is controlled to be 8-10 cm; the wood stick is used for punching firstly to prevent the cut from peeling and to compact the cutting so as to ensure that the cutting slips can be fully contacted with the root substrate. The cutting density is 10cm multiplied by 8 cm.
6. Post-cuttage management
And the management after cuttage is that rotary spraying is started after cuttage, water spraying time is 3 hours per day, and the temperature in the greenhouse is controlled to be 20-25 ℃.
Second, experimental results
Compared with the method that the top end of the cutting spike is not sealed, the wax sealing is carried out on the top end of the cutting spike, so that the number of roots, the length and the thickness of the roots are obviously improved, and the seedling forming rate of the cutting spike is greatly improved; compared with the method of soaking for 1 hour, 3 hours and 6 hours, the base part of the cutting shoot is soaked in the 3721 rooting powder diluted by 500 times for one night, so that the forming speed and the quantity of the root system are greatly accelerated, the germination of the branch is promoted in advance, and the survival rate is improved.
Through annual healthy and strong branches, a proper growth regulator, the concentration and the treatment time of the growth regulator, the rooting rate of actinidia arguta can reach 80%, and the phenomena of slow rooting and less rooting of actinidia arguta in cuttage are changed. Seedlings grown in the greenhouse can be transplanted in the field in march. The method is efficient and low in cost, and is beneficial to large-scale popularization of the good actinidia arguta variety.
Claims (3)
1. The asexual cutting propagation method of actinidia arguta is characterized by comprising the following steps of:
cutting time
The collection time of the branches is 10-1 month, and annual growing branches which are strong in growth, free of disease spots and full in lateral buds are selected;
② seedbed preparation and cuttage environment
Preparing a substrate from humus soil and vermiculite exposed to the sun;
thirdly, selection of cutting slips and treatment of cutting branches
Firstly, wrapping collected branches in a 4-degree refrigerator by using wet cloth and placing for 10-15 days; then taking out the branches, cutting the branches into 20-25cm cuttings containing 3-5 robust axillary buds, and sealing the tops of the cuttings with wax or plastic films;
pretreatment of growth regulator
Soaking the base part of the cutting in a rooting powder solution overnight;
fifthly, cutting
Firstly, punching with a wood stick with the same thickness, and compacting after cuttage, wherein the cuttage depth is 8-10 cm;
management after cuttage
After cuttage, the temperature in the greenhouse is controlled to be 20-25 ℃, and the relative humidity of the environment is controlled to be 80% -90%.
2. The asexual cutting propagation method of actinidia arguta according to claim 1, wherein humus soil and vermiculite are prepared according to a volume ratio of 9:1 in the second step.
3. The asexual cuttage propagation method for actinidia arguta according to claim 1, wherein the dilution multiple of the rooting powder in the step (iv) is 500 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011431401.4A CN112470730A (en) | 2020-12-07 | 2020-12-07 | Asexual cuttage propagation method for actinidia arguta |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011431401.4A CN112470730A (en) | 2020-12-07 | 2020-12-07 | Asexual cuttage propagation method for actinidia arguta |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112470730A true CN112470730A (en) | 2021-03-12 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011431401.4A Pending CN112470730A (en) | 2020-12-07 | 2020-12-07 | Asexual cuttage propagation method for actinidia arguta |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112470730A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113973598A (en) * | 2021-10-29 | 2022-01-28 | 长春大学 | Kiwi fruit hardwood cutting method suitable for Jilin province |
| CN114885688A (en) * | 2022-04-25 | 2022-08-12 | 西北农林科技大学 | Kiwi fruit micro-root grafting and cuttage method |
| CN115152442A (en) * | 2022-07-27 | 2022-10-11 | 福建省农业科学院生物技术研究所 | Cuttage method for golden beans |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102138482A (en) * | 2011-02-28 | 2011-08-03 | 中国科学院武汉植物园 | Method for carrying out asexual cuttage propagation on vernicia fordii |
| CN104067842A (en) * | 2014-07-17 | 2014-10-01 | 四川农业大学 | Cutting seedling method of branches dormant in winter |
| CN106069674A (en) * | 2016-06-26 | 2016-11-09 | 陕西省西安植物园 | A kind of method for quickly breeding being applicable to multiple Kiwifruit Cultivars |
| CN106258765A (en) * | 2016-09-20 | 2017-01-04 | 四川省益诺仕农业科技有限公司 | A kind of method of Actinidia arguta Sieb.et Zucc cutting propagation |
| CN109429853A (en) * | 2018-10-12 | 2019-03-08 | 四川省益诺仕农业科技有限公司 | A kind of Propagation of Actinidia Arguta By Green Cutting method |
-
2020
- 2020-12-07 CN CN202011431401.4A patent/CN112470730A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102138482A (en) * | 2011-02-28 | 2011-08-03 | 中国科学院武汉植物园 | Method for carrying out asexual cuttage propagation on vernicia fordii |
| CN104067842A (en) * | 2014-07-17 | 2014-10-01 | 四川农业大学 | Cutting seedling method of branches dormant in winter |
| CN106069674A (en) * | 2016-06-26 | 2016-11-09 | 陕西省西安植物园 | A kind of method for quickly breeding being applicable to multiple Kiwifruit Cultivars |
| CN106258765A (en) * | 2016-09-20 | 2017-01-04 | 四川省益诺仕农业科技有限公司 | A kind of method of Actinidia arguta Sieb.et Zucc cutting propagation |
| CN109429853A (en) * | 2018-10-12 | 2019-03-08 | 四川省益诺仕农业科技有限公司 | A kind of Propagation of Actinidia Arguta By Green Cutting method |
Non-Patent Citations (1)
| Title |
|---|
| 姚凯;: "软枣猕猴桃嫩枝扦插繁育技术" * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113973598A (en) * | 2021-10-29 | 2022-01-28 | 长春大学 | Kiwi fruit hardwood cutting method suitable for Jilin province |
| CN114885688A (en) * | 2022-04-25 | 2022-08-12 | 西北农林科技大学 | Kiwi fruit micro-root grafting and cuttage method |
| CN114885688B (en) * | 2022-04-25 | 2024-04-02 | 西北农林科技大学 | Kiwi fruit micro-root grafting cutting method |
| CN115152442A (en) * | 2022-07-27 | 2022-10-11 | 福建省农业科学院生物技术研究所 | Cuttage method for golden beans |
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Application publication date: 20210312 |