CN110050598B - Seedling growing method for improving grafting survival rate of Phoebe bournei - Google Patents

Seedling growing method for improving grafting survival rate of Phoebe bournei Download PDF

Info

Publication number
CN110050598B
CN110050598B CN201910392190.9A CN201910392190A CN110050598B CN 110050598 B CN110050598 B CN 110050598B CN 201910392190 A CN201910392190 A CN 201910392190A CN 110050598 B CN110050598 B CN 110050598B
Authority
CN
China
Prior art keywords
grafting
scion
stock
bud
phoebe bournei
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201910392190.9A
Other languages
Chinese (zh)
Other versions
CN110050598A (en
Inventor
李娟�
林建勇
谭文婧
梁瑞龙
蒋燚
余代渊
姜英
李跃勋
刘雄盛
刘菲
王勇
韦铄星
黄荣林
欧阳春华
戴菱
吴建坤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangxi Zhuang Autonomous Region Forestry Research Institute
Original Assignee
Guangxi Zhuang Autonomous Region Forestry Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangxi Zhuang Autonomous Region Forestry Research Institute filed Critical Guangxi Zhuang Autonomous Region Forestry Research Institute
Priority to CN201910392190.9A priority Critical patent/CN110050598B/en
Publication of CN110050598A publication Critical patent/CN110050598A/en
Application granted granted Critical
Publication of CN110050598B publication Critical patent/CN110050598B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting

Abstract

The invention discloses a seedling growing method for improving grafting survival rate of phoebe bournei, which comprises the procedures of stock cultivation, scion mother tree selection, scion strip preservation, grafting and post-grafting management, wherein tissue culture seedlings of phoebe bournei cultivated for 6-8 months are used as stocks, fresh sprouts taken out in season on the phoebe bournei mother trees are collected as scions, grafting is carried out by adopting a single-bud cutting grafting method before and after spring beginning when buds of phoebe bournei winter buds are not unfolded, and the grafted stocks are transplanted to a nursery to be subjected to post-grafting management, so that a complete new plant is obtained. The selected grafting stock is a phoebe bournei tissue culture seedling, and a single-bud cutting grafting method is adopted for grafting, so that the root system quality of the stock is good, the survival rate is high, the grafted seedling grows rapidly, and the seedling raising time is shortened; the grafted seedlings are utilized to establish a good clone seed garden to produce a large amount of high-quality Phoebe bournei seeds, the problem of limited bearing capacity of a good single plant is solved, a foundation is laid for cultivating high-quality nursery stocks and building high-quality artificial forests, and the good economic benefit, the good environmental benefit and the good ecological benefit are achieved.

Description

Seedling growing method for improving grafting survival rate of Phoebe bournei
Technical Field
The invention belongs to the technical field of forestry plant cultivation, relates to a Phoebe bournei seedling cultivation technology, and particularly relates to a seedling cultivation method for grafting Phoebe bournei.
Background
Minnan (a Chinese character of Min)Phoebe bournei) Is evergreen arbor of Lauraceae (Lauraceae), is a Chinese special tree species, and is a national II-level wild protective plant. The tree trunk is tall, big and straight, the wood texture structure is beautiful, the fragrance is corrosion resistant, and the wood is a precious material for high-grade buildings, furniture, sculptures and precise wood molds.
At present, the Phoebe bournei is generally planted with seedling, but the natural Phoebe bournei forest can blossom and fruit in more than 10 years, and the fruit bearing has obvious big and small year phenomena, and is not strong or is little strong in a small year. In addition, people excessively fell and utilize the phoebe bournei in the natural forest for a long time, and the germination rate of the phoebe bournei seeds and the survival rate of seedlings in the field are very low due to large environmental stress, so that the number of natural populations of the phoebe bournei is reduced, and the quality of phoebe bournei resources is sharply reduced; on the other hand, people pay less attention to the artificial cultivation work of the phoebe bournei, so that the existing phoebe bournei resources are very deficient, and the backup resources are seriously insufficient. In order to protect germplasm resources of the tree species and expand the distribution range of the phoebe bournei, and finally to reasonably develop and utilize the phoebe bournei, research workers have developed research works such as artificial cultivation of the phoebe bournei. In the process of constructing a phoebe bournei seed garden and collecting and storing gene resources, grafting is a common and important technical means. The conventional grafting method generally uses a phoebe bournei seedling as a stock, and adopts the traditional cutting grafting method and bud grafting method to carry out grafting, so that the survival rate is about 30 percent, and the survival rate is lower; meanwhile, due to the limitation of seasons on the cultivation of the phoebe bournei seedlings, the optimal grafting time is missed when the seedlings grow to the grafting requirement.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides the seedling raising method which can effectively shorten the cultivation time and improve the grafting survival rate of the phoebe bournei at low cost.
In order to achieve the above object, the technical solution of the present invention is as follows:
a seedling growing method for improving grafting survival rate of Phoebe bournei is characterized in that: the method comprises the steps of stock cultivation, scion mother tree selection, scion strip preservation, grafting and post-grafting management, wherein a tissue culture seedling of the phoebe bournei cultivated for 6-8 months is used as a stock, fresh sprouts taken out in the current season on the scion mother tree are collected and used as scions, a single-bud cutting method is adopted for grafting before and after spring, when winter buds of the phoebe bournei are not unfolded, the grafted stock is transplanted to a nursery for post-grafting management, and the main operation steps are as follows:
(1) stock cultivation: selecting a phoebe bournei tissue culture seedling which is robust in growth, good in growth vigor and free of diseases and insect pests, cultivating the phoebe bournei tissue culture seedling for 6-8 months according to a conventional method, transplanting the phoebe bournei tissue culture seedling into a seedling bed with a matrix I, then managing according to the conventional seedling cultivation method, and taking the phoebe bournei tissue culture seedling as a stock for later use when the seedling recovers to grow and a new tip is extracted after one month;
(2) selecting the scion mother tree: selecting excellent single plants which are robust in growth, straight in trunk and thick in tender tips as scion stock trees before and after spring and before bud of the phoebe bournei is not unfolded in a superior variety source region of the phoebe bournei;
(3) selecting scions: selecting fresh sprout strips extracted in the current season on the scion mother tree;
(4) and (4) spike strip preservation: cutting the collected sprout strips according to the length of 20-30cm to be used as scions, and bundling; bundling with wet towel, horizontally placing, and transporting in a sealed box; after the seedlings reach the nursery, unfastening the binding ropes, vertically placing the sprout strips in a sealed box with a cover, covering the sprout strips with a wet towel, filling clear water with the depth of 2-3cm in the sealed box, and placing the sealed box in a cool place for later use;
(5) grafting: before and after spring, grafting is carried out when the bud buds of winter buds of the phoebe bournei are not unfolded and the nursery stocks used as the stocks are not germinated; cutting the stock into 20-25cm high, and grafting by adopting a single-bud cutting and grafting method; selecting a thick and smooth part of the stock bark, and vertically cutting off 2-3cm downwards between the bark layer and the xylem by a knife; selecting a plump bud from the scion, internally cutting the bud to a position 1-2cm below the bud to a position between the cortex and the xylem by a cutter, then cutting the bud to the bottom in parallel, cutting a long cutting surface to be 2-3cm long, reversing the scion, and flatly cutting the back surface at a position 0.5cm away from the bud to a position between the cortex and the xylem; inserting the long cutting surface of the scion inwards into the cut of the stock, and tightly connecting stock and scion forming layers; then, a brand-new plastic film belt is tightly wound on the grafting opening, and the grafting opening is tightly bound without touching the scion during binding; then, the grafted plant is sleeved by a zipper type self-sealing bag from top to bottom, the distance between the top of the self-sealing bag and the scion bud is 2-3cm, and the bottom of the self-sealing bag exceeds the grafting opening by 2-3 cm;
(6) and (3) management after grafting: transplanting the grafted stock to a nursery land filled with a matrix II, putting the stock into a ridge, and covering a plastic shed to completely seal the nursery land so as to keep the nursery land moist without water accumulation; if the temperature in the nursery land exceeds 32 ℃, opening the plastic sheds at the two ends of the nursery land for heat dissipation, spraying water in the nursery land at the evening to replenish water, covering the plastic sheds, and totally closing again; inspecting the sprouting and growing conditions of the scions, and removing the plastic shed on the outer cover when the scion wound is basically healed; after 15-20 days, puncturing the self-sealing bag sleeved at the top end of the nursery stock by using a needle; and after the scion wound is completely healed, loosening the plastic film belt bound on the nursery stock, and performing water management according to a conventional method.
The matrix I in the step (1) is prepared by uniformly mixing loess, peat soil, carbonized chaff and organic fertilizer according to the volume ratio of 6:2:1: 1.
The diameter of the sprout in the step (3) is 0.2-0.3cm, the sprout is light green and semi-lignified, the leaves are fine and light green, and the bud is not opened yet.
Timely grafting the collected scion wood in the step (4), vertically placing the scion wood which cannot be grafted timely in a container filled with clear water of 2-3cm, and grafting after cutting off the end part immersed in the clear water by 5cm when grafting.
The substrate II in the step (6) is prepared by uniformly mixing surface soil, sufficient fermentation mushroom dregs and chaff according to the volume ratio of 6:3: 1.
Compared with the prior art, the invention has the following advantages and beneficial effects:
1. the selected grafting stock is a phoebe bournei tissue culture seedling, the root system is developed, the growth is rapid, the tissue culture seedling is transplanted into a substrate I which is prepared according to the growth characteristics and has high air permeability, water retention and fertilizer efficiency for cultivation, the requirement of the stock required by grafting can be met in a short time, the activity is strong, the metabolism is vigorous, the healing of a grafting wound is facilitated, and the grafting survival rate is greatly improved; after the grafting survives, because the root system quality of the stock is good, the grafted seedling grows rapidly, the seedling time is shortened, and the seedling time is further shortened.
2. According to the method, the excellent single plants which grow robustly and have straight trunks and thick young tips before the bud buds of the phoebe bournei are unfolded are selected as the scion-collecting mother trees, the fresh sprouts extracted in the season are selected as the scions, and the selected branches are high in lignification degree and activity and beneficial to improvement of the grafting survival rate.
3. The invention effectively preserves, transports and manages the collected scion, greatly reduces the transportation time and cost, simultaneously reduces the water loss of the scion, ensures the vitality of the scion and improves the production efficiency.
4. The invention adopts the single-bud cutting grafting method for grafting, so that the left cambium, the right cambium, the upper cambium and the lower cambium of the stock and the scion can be connected, and the calluses can be quickly connected after being formed; because the phoebe bournei has fewer sprouts suitable for making scion, the single-bud cutting and grafting method can save scion, meanwhile, the diameter of the scion and the diameter of the stock adopted by the invention are smaller, the two are basically consistent in size, and the grafting survival rate is improved by adopting the single-bud cutting and grafting method; after grafting, the seedling is sleeved with the zipper type self-sealing bag, so that the growth of the seedling is not influenced, the infection probability of a grafting port can be effectively reduced, and the survival rate of branch grafting is further improved.
5. According to the growth characteristics of the grafted Phoebe bournei seedlings, the matrix II with low cost and strong water retention is prepared, so that the grafted Phoebe bournei seedlings are thick and strong in root systems, good in seedling quality and capable of enhancing the afforestation effect.
6. According to the method, the grafted seedlings are used for establishing the excellent clone seed garden to produce a large number of high-quality phoebe bournei seeds, the problem that the bearing capacity of an excellent single plant is limited is solved, a foundation is laid for cultivating high-quality nursery stocks and building high-quality artificial forests, and the method has good economic benefits, environmental benefits and ecological benefits.
Detailed Description
The present invention is further illustrated by the following specific examples.
Example 1:
selecting a phoebe bournei tissue culture seedling which is strong in growth, good in growth vigor and free of diseases and insect pests, cultivating the phoebe bournei tissue culture seedling for 6-8 months according to a conventional method, transplanting the phoebe bournei tissue culture seedling into a seedling bed provided with a matrix I formed by uniformly mixing yellow heart soil, peat soil, carbonized chaff and an organic fertilizer according to a volume ratio of 6:2:1:1, and then managing according to a conventional seedling cultivation method; and after one month, taking the seedlings as the stocks for later use when the seedlings grow again and new shoots are extracted. In a natural forest of the phoebe bournei in the excellent variety source region of the phoebe bournei, before and after spring and before bud of the phoebe bournei is not unfolded, selecting excellent single plants which are robust in growth, straight in trunk and stout as scion stock trees. Selecting fresh sprout strips extracted in the current season on the scion mother tree; the diameter of the sprout is 0.2-0.3cm, the sprout is light green and semi-lignified, the leaves are fine and light green, and the bud is not opened yet.
Cutting the collected sprout strips according to the length of 20cm to be used as scions, and bundling; bundling with wet towel, horizontally placing, and transporting in a sealed box; after the seedlings reach a nursery, unfastening the binding ropes, vertically placing the sprout strips in a sealed box with a cover, covering the sprout strips with a wet towel, filling clear water with the depth of 2cm in the sealed box, and placing the sealed box in a shade place for later use; timely grafting the picked scion, vertically placing the scion which cannot be grafted in time in a container filled with 2cm of clear water, and grafting after cutting off 5cm of end part immersed in the clear water when grafting.
Before and after spring, grafting is carried out when the bud buds of winter buds of the phoebe bournei are not unfolded and the nursery stocks used as the stocks are not germinated; cutting the stock into 20cm high, and grafting by adopting a single-bud cutting method; selecting a thick and smooth part of the stock bark on the stock, and vertically cutting the part between the bark layer and the xylem by 2cm downwards by using a knife; selecting a plump bud from the scion, internally cutting the bud to a position 1cm below the bud by a cutter to a position between the cortex and the xylem, then cutting the bud to the bottom in parallel, cutting a long cutting surface to be 2cm long, reversing the scion, and then flatly cutting the back surface at a position 0.5cm away from the bud to a position between the cortex and the xylem; inserting the long cutting surface of the scion inwards into the cut of the stock, and tightly connecting stock and scion forming layers; then, a brand-new plastic film belt is tightly wound on the grafting opening, and the grafting opening is tightly bound without touching the scion during binding; then, the grafted plant is sleeved by a zipper type self-sealing bag from top to bottom, the distance between the top of the self-sealing bag and the scion bud is 2-3cm, and the bottom of the self-sealing bag exceeds the grafting opening by 2-3 cm.
Transplanting the grafted stock into a nursery land filled with a matrix II formed by uniformly mixing surface soil, sufficient fermentation fungus residues and chaff according to the volume ratio of 6:3:1, putting the nursery land into a furrow, and covering a plastic shed to fully seal the nursery land so as to keep the nursery land moist without water accumulation; if the temperature in the nursery land exceeds 32 ℃, opening the plastic sheds at the two ends of the nursery land for heat dissipation, spraying water in the nursery land at the evening to replenish water, covering the plastic sheds, and totally closing again; inspecting the sprouting and growing conditions of the scions, and removing the plastic shed on the outer cover when the scion wound is basically healed; after 15-16 days, puncturing the self-sealing bag sleeved at the top end of the nursery stock by using a needle; and after the scion wound is completely healed, loosening the plastic film belt bound on the nursery stock, and performing water management according to a conventional method. The grafting survival rate reaches 91.5 percent.
Example 2:
selecting a phoebe bournei tissue culture seedling which is robust in growth, good in growth vigor and free of diseases and insect pests, cultivating 7 months by a conventional method, transplanting the phoebe bournei tissue culture seedling into a seedling bed provided with a matrix I formed by uniformly mixing yellow heart soil, peat soil, carbonized chaff and an organic fertilizer according to the volume ratio of 6:2:1:1, and then managing by a conventional seedling cultivation method; and after one month, taking the seedlings as the stocks for later use when the seedlings grow again and new shoots are extracted. In a natural forest of the phoebe bournei in the excellent variety source region of the phoebe bournei, before and after spring and before bud of the phoebe bournei is not unfolded, selecting excellent single plants which are robust in growth, straight in trunk and stout as scion stock trees. Selecting fresh sprout strips extracted in the current season on the scion mother tree; the diameter of the sprout is 0.2-0.3cm, the sprout is light green and semi-lignified, the leaves are fine and light green, and the bud is not opened yet.
Cutting the collected sprout strips according to the length of 25cm to be used as scions, and bundling; bundling with wet towel, horizontally placing, and transporting in a sealed box; after the seedlings reach a nursery, unfastening the binding ropes, vertically placing the sprout strips in a sealed box with a cover, covering the sprout strips with a wet towel, filling clear water with the depth of 2cm in the sealed box, and placing the sealed box in a shade place for later use; timely grafting the picked scion, vertically placing the scion which cannot be grafted in time in a container filled with 2cm of clear water, and grafting after cutting off 5cm of end part immersed in the clear water when grafting.
Before and after spring, grafting is carried out when the bud buds of winter buds of the phoebe bournei are not unfolded and the nursery stocks used as the stocks are not germinated; cutting the stock into a height of 23cm, and grafting by adopting a single-bud cutting and grafting method; selecting a thick and smooth part of the stock bark on the stock, and vertically cutting the part between the bark layer and the xylem by 2cm downwards by using a knife; selecting a plump bud from the scion, internally cutting the bud to a position 1cm below the bud by a cutter to a position between the cortex and the xylem, then cutting the bud to the bottom in parallel, cutting a long cutting surface to be 2cm long, reversing the scion, and then flatly cutting the back surface at a position 0.5cm away from the bud to a position between the cortex and the xylem; inserting the long cutting surface of the scion inwards into the cut of the stock, and tightly connecting stock and scion forming layers; then, a brand-new plastic film belt is tightly wound on the grafting opening, and the grafting opening is tightly bound without touching the scion during binding; then, the grafted plant is sleeved by a zipper type self-sealing bag from top to bottom, the distance between the top of the self-sealing bag and the scion bud is 2-3cm, and the bottom of the self-sealing bag exceeds the grafting opening by 2-3 cm.
Transplanting the grafted stock into a nursery land filled with a matrix II formed by uniformly mixing surface soil, sufficient fermentation fungus residues and chaff according to the volume ratio of 6:3:1, putting the nursery land into a furrow, and covering a plastic shed to fully seal the nursery land so as to keep the nursery land moist without water accumulation; if the temperature in the nursery land exceeds 32 ℃, opening the plastic sheds at the two ends of the nursery land for heat dissipation, spraying water in the nursery land at the evening to replenish water, covering the plastic sheds, and totally closing again; inspecting the sprouting and growing conditions of the scions, and removing the plastic shed on the outer cover when the scion wound is basically healed; after 16-18 days, puncturing the self-sealing bag sleeved at the top end of the nursery stock by using a needle; and after the scion wound is completely healed, loosening the plastic film belt bound on the nursery stock, and performing water management according to a conventional method. The grafting survival rate reaches 92.1 percent.
Example 3:
selecting a phoebe bournei tissue culture seedling which is strong in growth, good in growth vigor and free of diseases and insect pests, cultivating the phoebe bournei tissue culture seedling for 6-8 months according to a conventional method, transplanting the phoebe bournei tissue culture seedling into a seedling bed provided with a matrix I formed by uniformly mixing yellow heart soil, peat soil, carbonized chaff and an organic fertilizer according to a volume ratio of 6:2:1:1, and then managing according to a conventional seedling cultivation method; and after one month, taking the seedlings as the stocks for later use when the seedlings grow again and new shoots are extracted. In a natural forest of the phoebe bournei in the excellent variety source region of the phoebe bournei, before and after spring and before bud of the phoebe bournei is not unfolded, selecting excellent single plants which are robust in growth, straight in trunk and stout as scion stock trees. Selecting fresh sprout strips extracted in the current season on the scion mother tree; the diameter of the sprout is 0.2-0.3cm, the sprout is light green and semi-lignified, the leaves are fine and light green, and the bud is not opened yet.
Cutting the collected sprout strips by 30cm in length to be used as scions, and bundling; bundling with wet towel, horizontally placing, and transporting in a sealed box; after the seedlings reach a nursery, unfastening the binding ropes, vertically placing the sprout strips in a sealed box with a cover, covering the sprout strips with a wet towel, filling clear water with the depth of 3cm in the sealed box, and placing the sealed box in a shade place for later use; timely grafting the picked scion, vertically placing the scion which cannot be grafted in time in a container filled with clear water of 3cm, and grafting after cutting off the end part immersed in the clear water by 5cm when grafting.
Before and after spring, grafting is carried out when the bud buds of winter buds of the phoebe bournei are not unfolded and the nursery stocks used as the stocks are not germinated; cutting the stock to be 25cm high, and grafting by adopting a single-bud cutting and grafting method; selecting a thick and smooth part of the stock bark on the stock, and vertically cutting 3cm downwards between the bark layer and the xylem by using a knife; selecting a plump bud from the scion, internally cutting the bud to a position 2cm below the bud by a cutter to a position between the cortex and the xylem, then cutting the bud to the bottom in parallel, cutting a long cutting surface to be 3cm long, reversing the scion, and flatly cutting the back surface to a position 0.5cm below the bud to a depth between the cortex and the xylem; inserting the long cutting surface of the scion inwards into the cut of the stock, and tightly connecting stock and scion forming layers; then, a brand-new plastic film belt is tightly wound on the grafting opening, and the grafting opening is tightly bound without touching the scion during binding; then, the grafted plant is sleeved by a zipper type self-sealing bag from top to bottom, the distance between the top of the self-sealing bag and the scion bud is 2-3cm, and the bottom of the self-sealing bag exceeds the grafting opening by 2-3 cm.
Transplanting the grafted stock into a nursery land filled with a matrix II formed by uniformly mixing surface soil, sufficient fermentation fungus residues and chaff according to the volume ratio of 6:3:1, putting the nursery land into a furrow, and covering a plastic shed to fully seal the nursery land so as to keep the nursery land moist without water accumulation; if the temperature in the nursery land exceeds 32 ℃, opening the plastic sheds at the two ends of the nursery land for heat dissipation, spraying water in the nursery land at the evening to replenish water, covering the plastic sheds, and totally closing again; inspecting the sprouting and growing conditions of the scions, and removing the plastic shed on the outer cover when the scion wound is basically healed; after 18-20 days, puncturing the self-sealing bag sleeved at the top end of the nursery stock by using a needle; and after the scion wound is completely healed, loosening the plastic film belt bound on the nursery stock, and performing water management according to a conventional method. The grafting survival rate reaches 92.6 percent.

Claims (1)

1. A seedling growing method for improving grafting survival rate of Phoebe bournei is characterized in that: the method comprises the steps of stock cultivation, scion mother tree selection, scion strip preservation, grafting and post-grafting management, wherein a tissue culture seedling of the phoebe bournei cultivated for 6-8 months is used as a stock, fresh sprouts taken out in the current season on the scion mother tree are collected and used as scions, a single-bud cutting method is adopted for grafting before and after spring, when winter buds of the phoebe bournei are not unfolded, the grafted stock is transplanted to a nursery for post-grafting management, and the main operation steps are as follows:
(1) stock cultivation: selecting a phoebe bournei tissue culture seedling which is robust in growth, good in growth vigor and free of diseases and insect pests, cultivating the phoebe bournei tissue culture seedling for 6-8 months according to a conventional method, transplanting the phoebe bournei tissue culture seedling into a seedling bed with a matrix I, then managing according to the conventional seedling cultivation method, and taking the phoebe bournei tissue culture seedling as a stock for later use when the seedling recovers to grow and a new tip is extracted after one month;
the matrix I is prepared by uniformly mixing yellow core soil, peat soil, carbonized chaff and organic fertilizer according to the volume ratio of 6:2:1: 1;
(2) selecting the scion mother tree: selecting excellent single plants which are robust in growth, straight in trunk and thick in tender tips as scion stock trees before and after spring and before bud of the phoebe bournei is not unfolded in a superior variety source region of the phoebe bournei;
(3) selecting scions: selecting fresh sprout strips extracted in the current season on the scion mother tree;
the diameter of the sprout is 0.2-0.3cm, the sprout is light green and semi-lignified, the leaves are fine and light green, and the bud is not opened;
(4) and (4) spike strip preservation: cutting the collected sprout strips according to the length of 20-30cm to be used as scions, and bundling; bundling with wet towel, horizontally placing, and transporting in a sealed box; after the seedlings reach the nursery, unfastening the binding ropes, vertically placing the sprout strips in a sealed box with a cover, covering the sprout strips with a wet towel, filling clear water with the depth of 2-3cm in the sealed box, and placing the sealed box in a cool place for later use; timely grafting the picked scion, vertically placing the scion which cannot be grafted in time in a container filled with clear water of 2-3cm, and grafting after cutting off 5cm of the end part immersed in the clear water when grafting;
(5) grafting: before and after spring, grafting is carried out when the bud buds of winter buds of the phoebe bournei are not unfolded and the nursery stocks used as the stocks are not germinated; cutting the stock into 20-25cm high, and grafting by adopting a single-bud cutting and grafting method; selecting a thick and smooth part of the stock bark, and vertically cutting off 2-3cm downwards between the bark layer and the xylem by a knife; selecting a plump bud from the scion, internally cutting the bud to a position 1-2cm below the bud to a position between the cortex and the xylem by a cutter, then cutting the bud to the bottom in parallel, cutting a long cutting surface to be 2-3cm long, reversing the scion, and flatly cutting the back surface at a position 0.5cm away from the bud to a position between the cortex and the xylem; inserting the long cutting surface of the scion inwards into the cut of the stock, and tightly connecting stock and scion forming layers; then, a brand-new plastic film belt is tightly wound on the grafting opening, and the grafting opening is tightly bound without touching the scion during binding; then, the grafted plant is sleeved by a zipper type self-sealing bag from top to bottom, the distance between the top of the self-sealing bag and the scion bud is 2-3cm, and the bottom of the self-sealing bag exceeds the grafting opening by 2-3 cm;
(6) and (3) management after grafting: transplanting the grafted stock to a nursery land filled with a matrix II, putting the stock into a ridge, and covering a plastic shed to completely seal the nursery land so as to keep the nursery land moist without water accumulation; if the temperature in the nursery land exceeds 32 ℃, opening the plastic sheds at the two ends of the nursery land for heat dissipation, spraying water in the nursery land at the evening to replenish water, covering the plastic sheds, and totally closing again; inspecting the sprouting and growing conditions of the scions, and removing the plastic shed on the outer cover when the scion wound is basically healed; after 15-20 days, puncturing the self-sealing bag sleeved at the top end of the nursery stock by using a needle; after the scion wound is completely healed, unfastening the plastic film belt bound on the nursery stock, and carrying out water management according to a conventional method;
the substrate II is prepared by uniformly mixing surface soil, sufficient fermentation mushroom dregs and chaff according to the volume ratio of 6:3: 1.
CN201910392190.9A 2019-05-13 2019-05-13 Seedling growing method for improving grafting survival rate of Phoebe bournei Active CN110050598B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910392190.9A CN110050598B (en) 2019-05-13 2019-05-13 Seedling growing method for improving grafting survival rate of Phoebe bournei

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910392190.9A CN110050598B (en) 2019-05-13 2019-05-13 Seedling growing method for improving grafting survival rate of Phoebe bournei

Publications (2)

Publication Number Publication Date
CN110050598A CN110050598A (en) 2019-07-26
CN110050598B true CN110050598B (en) 2021-09-07

Family

ID=67322942

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910392190.9A Active CN110050598B (en) 2019-05-13 2019-05-13 Seedling growing method for improving grafting survival rate of Phoebe bournei

Country Status (1)

Country Link
CN (1) CN110050598B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110447403B (en) * 2019-08-13 2022-04-22 中国林业科学研究院亚热带林业研究所 Magnolia officinalis grafting propagation method
ES2940442B2 (en) * 2021-11-04 2023-11-02 Silberum S L CULTIVATION PROCEDURE BY GRAFTING FOREST TREES

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104904500B (en) * 2015-06-01 2017-07-14 广西壮族自治区林业科学研究院 A kind of engrafting method using masson pine tissue-cultured seedling as stock
CN104885859B (en) * 2015-06-16 2018-04-03 黄达龙 The method for culturing seedlings of thornbuss
CN106234110A (en) * 2016-07-29 2016-12-21 广西壮族自治区林业科学研究院 A kind of Phoebe bournei grafting seedlings-growing method
CN106818228A (en) * 2017-01-21 2017-06-13 庆元县实验林场 It is a kind of to improve the method that Phoebe bournei breeding garden improves graft survival rate
CN106613377A (en) * 2017-02-21 2017-05-10 浙江农林大学 Ancient phoebe zherman grafting, breeding and preserving method

Also Published As

Publication number Publication date
CN110050598A (en) 2019-07-26

Similar Documents

Publication Publication Date Title
CN102884924B (en) Rapid container seedling culturing method for peach
CN101473747B (en) Micro-graft and fast propagation method of tree
CN105746224B (en) A kind of kalopanax septemlobus seed is nursed young plants in hothouses method
CN107996166B (en) Rapid cuttage breeding method for catalpa bungei
CN103270920A (en) Kiwi seedling cultivation method
Hernández et al. Cultivation systems
CN102318546B (en) Method for growing container seedlings of cork oak in winter
CN111466216B (en) Multi-branch inarching one-time forming method for intercropping transformation of sealed apple orchard
CN112602522A (en) Method for grafting germ roots of small camellia oleifera seedlings
CN110050598B (en) Seedling growing method for improving grafting survival rate of Phoebe bournei
CN107094576A (en) A kind of efficiently breeding sequoia sempervirens cuttage seedling-growing method
CN110622814B (en) Method for cultivating camellia bonsai by grafting camellia oleifera tree roots
CN110278822B (en) Method for cultivating peach trees in high-altitude areas
CN111657029A (en) Improved cleft grafting jujube grafting cultivation method
CN109769489B (en) High-position grafting method for multiple varieties of pumpkins
CN115349362B (en) Method for grafting and raising seedlings of tung oil tree buds
CN110876307A (en) Grafting method for dwarfing Chinese chestnut
CN111955333B (en) Cultivation method of large container seedlings of carya illinoensis fruiting bodies
CN107548858A (en) A kind of apple nursery stock mating system
CN111226672B (en) Lycium ruthenicum seedling culture and afforestation method
CN109744040A (en) A kind of cultivation of container seedling technology of the salt tolerant grafting rose of Sharon
CN112425390A (en) Peach tree grafting planting method
CN113317099A (en) Cherry round-table type close planting cultivation technology suitable for greenhouse cultivation
CN113317100A (en) Cherry round-table type close planting cultivation technology
CN106718096B (en) Rapid garden building method for grafting and planting kiwi fruits

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
EE01 Entry into force of recordation of patent licensing contract

Application publication date: 20190726

Assignee: Wuzhou Zhibang Agroforestry Technology Co.,Ltd.

Assignor: GUANGXI ZHUANG AUTONOMOUS REGION FORESTRY Research Institute

Contract record no.: X2023980045109

Denomination of invention: A Seedling Raising Method for Improving the Survival Rate of Minnan Grafting

Granted publication date: 20210907

License type: Common License

Record date: 20231031

EE01 Entry into force of recordation of patent licensing contract