CN106613377A - Ancient phoebe zherman grafting, breeding and preserving method - Google Patents
Ancient phoebe zherman grafting, breeding and preserving method Download PDFInfo
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- CN106613377A CN106613377A CN201710092428.7A CN201710092428A CN106613377A CN 106613377 A CN106613377 A CN 106613377A CN 201710092428 A CN201710092428 A CN 201710092428A CN 106613377 A CN106613377 A CN 106613377A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
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Abstract
The invention discloses an ancient phoebe zherman grafting, breeding and preserving method. The method comprises the following steps that firstly, between December and February next year, current-year branches on the middle upper parts of ancient phoebe zherman (more than 200 years old big trees including phoebe chekiangensis and phoebe bournei) are collected before trees bud, after a part of leaves are removed, the branches are moisturized and preserved with wet paper, brought back and stored in wet sand for preservation; secondly, the grafting time is the time when rootstocks start to bud in February and March and scions do not bud; thirdly, current-year pruning branches in a length of about 20 cm are adopted to serve as the scions; fourthly, annual to biennial container-grown seedlings serve as the rootstocks, and grafting is conducted by means of the cut-grafting method; fifthly, after grafting is conducted, the seedlings and the containers are both temporarily planted into soil, and arched sheds are constructed with plastic films covering for moisturizing; sixthly, an observation is conducted once every 7-10 days, the phenomenon that there is water mist or water droplets on the films is taken as a standard, and water is properly sprayed, so that humidness in the sheds is kept. New buds come up in about 30 days, the films on the sheds are uncovered, normal water and fertilizer management is conducted, the grafting survival rate reaches 82%, the length of annual grafted seedlings can reach 30-40 cm, growth is normal, and branches and leaves can keep the characteristics of original plants (ancient phoebe zherman).
Description
Technical field
The invention belongs to plant biotechnology field, and in particular to a kind of ancient nanmu grafting and breeding store method.
Background technology
Phoebe chekiangensis (Phoebe chekiangensis), Phoebe bournei (P.bournei) belong to the high megaphanerophyte of Lauraceae Phoebe, are
One of the foundation seed kind of " silkwood ", is the peculiar rare tree in subtropical zone with very high economic worth, is divided naturally
It is distributed in the ground such as Zhejiang, Fujian, Jiangxi, Hunan, Hubei, Anhui, Guangxi, Guangdong, Guizhou (Chinese Academy of Sciences Chinese Plants will editor
The committee, 1982；Anhui flora cooperative groups, 1986).Its trunk is logical straight, and material is graceful, is that the special type that top-grade furniture makes is used
Material；Tree body is tall and big, and the four seasons are emerald green, is also afforestation fine tree species.Although the economy of Phoebe chekiangensis, Phoebe bournei and ornamental value are high,
But available resources amount is few, survived in various places Feng Shui Woodland or temple with ancient and well-known trees more, effective original place protection expands numerous guarantor with strange land
Deposit such ancient nanmu remaining germ plasm resource and become and be imminent.Research before focuses mostly in seed dormancy (Shi Xiaohua and history
Loyal gift 1990), (leaf Kingsoft 2013, Wang Dongguang 2013, Luo Chun are flat 2015, Shen Zhan for treelet or nursery stock Cutting test and tissue cultures
2013rd, Qu Fenxia 2010 etc.), cultivating seedlings (Li Donglin and to its cypress 2004,2006, Wang Yi etc. 2013, Liu Zhiqiang 2001 etc.
Deng), population bio-diversity (it is old come moral 2012, Wu great Rong 2001,2003, Zhou Xiangfeng 2005, king is logical 2000, Liu's treasured 2005
Deng), artificial forest cultivation (Shao Ming dawn 2014, king's Golden Shield 2015, Hu Yongyan 2012, Liu's treasured 2013, Ou Jiande 2015, Peng Longfu,
2003；Wu's load screen-like mountain peak, 2005 etc.) and ecological (Ma Mingdong etc., 2008；Wang Qi etc., 2013, Zou Hui Chongqing 1995, Wu great Rong 1997,
Zou Xiu red 2002 etc.) etc. aspect, and how to utilize vegetative propagation technique, effectively breed the Gu nanmu such as Phoebe chekiangensis ancient tree, Phoebe bournei ancient tree
Wooden genotype but has no report.
Phoebe chekiangensis, Phoebe bournei belong to cross pollinated species, mainly by seminal propagation, by gathering its seed growing into freely awarding
Powder offspring, the not former pnca gene type that factor generation separates and preserves.Accordingly, it would be desirable to using vegetative manner, by original gene
Type is preserved.Existing research only relates to carry out cuttage or Tissue culture assays using nanmu filial generation seedling.And it is big to be directed to centuries
Tree, ancient tree nanmu, physiological age is big, its ability decline of blossoming and bearing fruit, and branch is aging, with it as cuttage or group training material one
As can not be successful.Recognize from reason, tissue cultures have the excellent hereditary capacity that reproduction speed is fast, can keep female parent, energy childrenization
The advantages of age of propagating materials, be to realize excellent germplasm breeding, the long-term optional approach for preserving, but big using degree of aging
Ancient nanmu branch, bud etc. when carrying out tissue and breeding, actual successfully chance is little.For this purpose, proposing grafting first preserves ancient nanmu
The method of wooden genotype simultaneously carries out correlation test.The method is also construction nanmu class seeds Grafting Seed Orchard to realize blooming ahead of time
As a result important method.
The content of the invention
In order to solve the above problems, the present invention provides a kind of ancient nanmu grafting and breeding store method.
The ancient nanmu grafting and breeding store method that the present invention is provided, it comprises the steps：
1) between 2 months December～next year, the current-year branch of the ancient nanmu tree crown middle and upper part of collection before bud is sprouted is set, goes to part
L Water Paper moisturizing after leaf is preserved and taken back, and is stored in wet sand preservation；
2) when grafting time starts to sprout for 2～March stock tree bud, and require that scion bud is not sprouted；
3) scion is made using the training of giving birth to then of 20～25cm；Cross long shoot and remove base portion, each fringe bar need to have terminal bud and phase
The lateral bud answered.
4) stock was made using 1～2 year raw Phoebe chekiangensis, Phoebe bournei container seedling, using cut-grafting method grafting；
5) involve grafting in a criminal case same container after grafting to heel in soil, it is ensured that grafting strain can absorb moisture to keep from soil
Growth conditions.Shed covered plastic film moisturizing is done, to ensure that it is withered that long branch scion is difficult to be dried.
6) per 7～10 days, observation once, had water smoke or little water droplet to spray as standard, suitably in water tariff collection canopy with film
Humidity, treats that sprouting grows and throw off in time film on canopy, carries out normal water and fertilizer management.
In one embodiment of the invention, young leaves starts after growth, and canopy is taken off in time, enriches foliage fertilizer to promote growth, to 6
The beginning of the month is put in cloudy canopy and is cultivated, and to reach the increment of 30-40cm then, cultivates into the grafting of stalwartness.
Wherein, when gathering fringe bar, remove most of blade, retain 4～5 leaves in branch top.
Wherein, stock requires robust growth, no disease and pests harm, and ground diameter is in more than 0.4cm.
Wherein, during grafting, retain the two half leaves in top, remove remaining blade.
Wherein, described ancient nanmu is Phoebe chekiangensis or Phoebe bournei big tree of the age of tree more than 200 years.
Using the engrafting method of the present invention, the graft survival rate of ancient nanmu is up to 82%.Annual grafting up to 30～
40cm, growth is normal, and branches and leaves keep former strain (ancient nanmu) feature.
Specific embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.
The different scion length of embodiment 1 carry out Grafting Experiment
In general the scion length of grafting is more short better, and majority retains 1 full bud for optimum.Many preliminary experiments of Jing
It was found that, for the fringe bar gathered on Phoebe chekiangensis, Phoebe bournei ancient tree, using conventional method grafting without can not survive.For this purpose, special visit
The impact of the scion to survival rate of different length is begged for.But as scion length increases, under the conditions of Routine Management, that is, Jing after connecing
Closed wrapping, be put under normal cool canopy cultivate, long branch soon can be slowly withered because of dehydration, though and the dehydration of brachyplast bar more slowly, shape
Difficult into healing, survival rate is not high.For this purpose, Jing follow-up management modes test improvement, the scion test of different length is carried out again.Knot
Fruit is shown in Table 1.
Impact of the different length scion of table 1 to ancient nanmu grafting survival
As shown in table 1, brachyplast grafting strain growth potential is substantially weak, and survival rate is also significantly low, and adopts natural length (portion big absolutely
Point) current-year branch carry out grafting, the bud of reservation is more, and general up to more than 5, each buds of Cheng Shaohou can grow, and integrally connect
Strain growth is vigorous.Result no significant difference on Phoebe chekiangensis, Phoebe bournei, perhaps this is related to the biological characteristics of the platymiscium.
The different parts fringe bar graft survival rate of embodiment 2
For ancient tree, Phoebe chekiangensis, Phoebe bournei all, as the age of tree increases its increase developmental age, tree growth potential by
Year declines, and becomes apparent from the growth for especially showing branches and leaves.The branch ability grafting for selecting where goes out surviving for more satisfaction
Rate and excellent nursery stock are the purposes of this test.
Test is picked up from the fringe bar at ancient nanmu bottom branch (collection facilitates the most), tree crown middle part and three positions in top and is carried out
The Grafting Experiment of similarity condition, test is carried out by conditions such as current growth branch, spring grafting and shed moisturizing management.The results are shown in Table
2。
The graft survival rate of the three position branches of ancient nanmu of table 2
As shown in Table 2, tree crown top branch graft survival rate highest, two seeds are all.The position shoot vegetative is supplied
Answer sufficient, light income big, growth potential is good, the relatively crude shape of branch, graft survival rate optimum (reaching as high as 92%).But for height
For big ancient nanmu, the position branch collection is very difficult, is many typically all to gather middle part branch, and its survival rate also can reach
Require to breeding and preserving.
The difference of embodiment 3 adopts bar time graft survival rate
In general, grafting fringe bar is with adopting with connecing beneficial to surviving.
This test branch and is tested with adopting branch after sprouting using not sprouting for storage, from terms of actual tests result,
The latter survives strain seldom (being shown in Table 3), without using value.
The different acquisition fringe bar time of table 3 affects on ancient nanmu grafting survival
The graft survival rate of the different grafting times of embodiment 4
Grafting time is one of key factor of impact survival rate, generally for the scion that the developmental age is big, its mitogenetic group
Knit less, power of regeneration it is poor, to reach its callus can be promoted to produce with greater need for best grafting time is inquired into.For this purpose, removing the spring
Quarterly test tests outer, has also carried out south of the River plum rains season and the test of late fall secondary grafting, all carries out long branch, container seedling stock and by rear
Bury soil plus shed moisturizing management.The results are shown in Table 4.As seen from Table 4, the plum rains season grafting at the beginning of 6 months, fringe bar with adopting with connecing, after connecing
Moisturizing management in shed continuously emerges bud, leaf and blacks because of temperature raising, and leaf comes off.Also sent out using the compatibility test of Routine Management
Existing bud is withered and easily dead.The season is easily formed callus, but humidity still causes difficulty to survive with the double influence of temperature.
The different grafting times of table 4 affect on ancient nanmu grafting survival
The difference of embodiment 5 connects the graft survival rate of rear way to manage
It is put under normal condition or hot-house culture after being wrapped with plastics binder during general grafting trees.The present invention is for being somebody's turn to do
Method graft survival rate is low, has carried out three kinds and has connect rear seedling way to manage：Bagging moisturizing after Routine Management (CK), grafting is processed and transferred
Soil is buried after connecing and heels in and add a cover film moisturizing process.Graft survival rate is counted after 1 month and the results are shown in Table 5.
As shown in Table 5, two seeds it is each 50 plants connect after carry out three kinds of process, two interspecific differences less, but three kinds of process
Between difference it is extremely notable.Not only survival rate is significantly improved under the conditions of shed moisture-heat preservation, and grows beginning early, and growing way is good, entirely
Year increment is also big.
The difference of table 5 connects rear way to manage to be affected on ancient nanmu grafting survival
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, on the premise of without departing from the technology of the present invention principle, continuation can also be made and improved, and grafting work and grafting work
Technology is closely related, and these improvement also should be regarded as protection scope of the present invention.
Claims (6)
1. a kind of ancient nanmu grafting and breeding store method, it comprises the steps：
1) between 2 months December～next year, the current-year branch of the ancient nanmu tree crown middle and upper part of collection before bud is sprouted is set, after removing part leaf
L Water Paper moisturizing is taken back, and is stored in wet sand preservation；
2) when grafting time starts to sprout for 2～March stock tree bud, and require that scion bud is not sprouted；
3) scion is made using the training of giving birth to then of 20～25cm；
4) stock was made using 1～2 year raw Phoebe chekiangensis, Phoebe bournei container seedling, using cut-grafting method grafting；
5) involve grafting in a criminal case same container after grafting to heel in soil, do shed covered plastic film moisturizing；
6) per 7～10 days, observation once, had water smoke or little water droplet to spray as standard, suitably wet in water tariff collection canopy with film
Degree, treats that sprouting grows and throw off in time film on canopy, carries out normal water and fertilizer management.
2. Gu nanmu grafting and breeding store method as claimed in claim 1, it is characterised in that young leaves starts after growth, in time
Canopy is taken off, foliage fertilizer is enriched to promote growth, is cultivated to being put in cloudy canopy at the beginning of 6 months, to reach the growth of 30-40cm then
Amount, cultivates into the grafting of stalwartness.
3. Gu nanmu grafting and breeding store method as claimed in claim 1, it is characterised in that during collection fringe bar, remove big portion
Leaflet piece, retains 4～5 leaves in branch top.
4. Gu nanmu grafting and breeding store method as claimed in claim 1, it is characterised in that stock requires robust growth, nothing
Pest and disease damage, ground diameter is in more than 0.4cm.
5. Gu nanmu grafting and breeding store method as claimed in claim 1, it is characterised in that during grafting, retain two, top
Half leaf, removes remaining blade.
6. Gu nanmu grafting and breeding store method as claimed in claim 1, it is characterised in that described ancient nanmu exists for the age of tree
The Phoebe chekiangensis of more than 200 years or the big tree of Phoebe bournei.
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| CN201710092428.7A CN106613377A (en) | 2017-02-21 | 2017-02-21 | Ancient phoebe zherman grafting, breeding and preserving method |
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| CN201710092428.7A CN106613377A (en) | 2017-02-21 | 2017-02-21 | Ancient phoebe zherman grafting, breeding and preserving method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108522051A (en) * | 2018-01-08 | 2018-09-14 | 江苏农牧科技职业学院 | A kind of purple nanmu ancient tree twig cleft grafting mating system |
| CN110050598A (en) * | 2019-05-13 | 2019-07-26 | 广西壮族自治区林业科学研究院 | A kind of method for culturing seedlings improving Phoebe bournei graft survival rate |
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| CN101406142A (en) * | 2008-11-14 | 2009-04-15 | 刘桂玉 | Method for grafting seedling of fruit tree in spring |
| CN104938218A (en) * | 2015-06-16 | 2015-09-30 | 湖北航发苗木园艺投资有限公司 | Method for grafting photinia davidsoniae to annatto photinia |
| CN105993618A (en) * | 2016-05-20 | 2016-10-12 | 芜湖欧标农业发展有限公司 | Method for grafting Photinia fraseri |
| CN106234110A (en) * | 2016-07-29 | 2016-12-21 | 广西壮族自治区林业科学研究院 | A kind of Phoebe bournei grafting seedlings-growing method |
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2017
- 2017-02-21 CN CN201710092428.7A patent/CN106613377A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101406142A (en) * | 2008-11-14 | 2009-04-15 | 刘桂玉 | Method for grafting seedling of fruit tree in spring |
| CN104938218A (en) * | 2015-06-16 | 2015-09-30 | 湖北航发苗木园艺投资有限公司 | Method for grafting photinia davidsoniae to annatto photinia |
| CN105993618A (en) * | 2016-05-20 | 2016-10-12 | 芜湖欧标农业发展有限公司 | Method for grafting Photinia fraseri |
| CN106234110A (en) * | 2016-07-29 | 2016-12-21 | 广西壮族自治区林业科学研究院 | A kind of Phoebe bournei grafting seedlings-growing method |
Non-Patent Citations (2)
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| 安徽教育出版社: "《劳动技术 初中一年级[M]》", 31 August 1991 * |
| 曾武、黎建伟等: "楠木小苗嫁接育苗技术", 《林业科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108522051A (en) * | 2018-01-08 | 2018-09-14 | 江苏农牧科技职业学院 | A kind of purple nanmu ancient tree twig cleft grafting mating system |
| CN110050598A (en) * | 2019-05-13 | 2019-07-26 | 广西壮族自治区林业科学研究院 | A kind of method for culturing seedlings improving Phoebe bournei graft survival rate |
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Application publication date: 20170510 |