CN106069674A - A kind of method for quickly breeding being applicable to multiple Kiwifruit Cultivars - Google Patents
A kind of method for quickly breeding being applicable to multiple Kiwifruit Cultivars Download PDFInfo
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- CN106069674A CN106069674A CN201610469723.5A CN201610469723A CN106069674A CN 106069674 A CN106069674 A CN 106069674A CN 201610469723 A CN201610469723 A CN 201610469723A CN 106069674 A CN106069674 A CN 106069674A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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Abstract
The invention belongs to technical field of plant propagation, particularly to a kind of method for quickly breeding being applicable to multiple Kiwifruit Cultivars, including propagating materials preparation, immersion, accelerating germination, urge root, point plant and the step such as seedling exercising.The present invention is applicable to Actinidia arguta Sieb.et Zucc, Rhesus Monkey in Shaanxi Province Fructus Persicae, the batch production fast-propagation of black stamen Fructus actinidiae chinensis, solves normal cutting propagation technology growing-seedling period length, technical problem that planting percent is low, the most also explores the asexual reproduction method with general applicability.The most only needed the time of about 15 days by the present invention, Actinidia arguta Sieb.et Zucc, Rhesus Monkey in Shaanxi Province Fructus Persicae, black stamen Fructus actinidiae chinensis branch section germination rooting rate up to 95% and more than, and growth of seedling is healthy and strong.The method operation is simple, it is easy to operation, a fado use, reduces seedling cost, and the operation of laminating nursery factory is actual, improves the overall efficiency of nursery factory.
Description
Technical field
The invention belongs to technical field of plant propagation, be specifically related to the side of a kind of applicable batch production Fast-propagation Fructus actinidiae chinensis
Method.This method is particularly suited for Actinidia arguta Sieb.et Zucc, Rhesus Monkey in Shaanxi Province Fructus Persicae, the Fast-propagation of black stamen Fructus actinidiae chinensis.
Background technology
Actinidia arguta Sieb.et Zucc, Rhesus Monkey in Shaanxi Province Fructus Persicae, black stamen Fructus actinidiae chinensis are Actinidiaceae Actinidia light fruit group plant.It is wild
Kind is distributed mainly on Japan, Korea S, Korea and east China, and also there is distribution in Russia.With Actinidia arguta Sieb.et Zucc, Rhesus Monkey in Shaanxi Province
Fructus Persicae, black stamen Fructus actinidiae chinensis wild varieties based on material, the country such as China, New Zealand, the U.S., Italy cultivates more than 10 product
Kind, its fruit light is without hair, direct-edible, nutritious, has pure green fruit, also has skin red meat green, also has pure aubergine
Kind.Actinidia mostly is climbing plant, is not only suitable for as application, is also suitable for ornamental plantation plantation, especially
It applies more prospect in harvesting garden.
Actinidia arguta Sieb.et Zucc, Rhesus Monkey in Shaanxi Province Fructus Persicae, the Fast-propagation of black stamen Fructus actinidiae chinensis are mainly cuttage, propagation by grafiting.Conventional skewer
Inserting in branch sand of gathering autumn to hide, cuttage in spring, the end of spring and the beginning of summer germinates.Normal cutting propagation, propagation by grafiting cycle are long, and rooting rate only exists
About 50%.Having been reported that the method using water to support cuttage twig, rootage duration was at about 30 days, and rooting rate is about 60%, and sent out
Bud is irregular, and slow Seedling is serious, does not reaches the requirement of factorial praluction.
Patent CN102177847A discloses the factorial seedling-culturing method of a kind of Actinidia arguta Sieb.et Zucc, joining including culture medium
Five steps transplanted by system, the choosing and sterilize, inoculating of outer implant, successive transfer culture, husky bed.But this method needs to configure composition
Complicated and culture medium configured the strict minimal medium of range request, inducing culture and subculture medium, and operating process is loaded down with trivial details,
Need to put into more manpower and equipment cost, not there is wide applicability.
Patent CN103749238A provides a kind of Actinidia arguta Sieb.et Zucc breed breeding and Fruiting And High Yield Cultivation Techniques, and to described
Technology has carried out detailed explanation.This patent procedure and practical, has reference, but described in this patent, technology is numerous
Trivial, it is not suitable for efficiency for the factorial praluction nursery pattern guided.
Patent CN102960185A relates to the engrafting method of a kind of actinidia tree, solves the age of tree Fructus actinidiae chinensis more than 5 years
Set and lead, present in grafting, the shortcoming that climing branch updating ability is poor, accelerate tree body Cultivar replacing replacement paces.This patent is more
Be suitable to the renewal of the veteran in field, not there is the directive significance of factorial praluction nursery.
Summary of the invention
Present invention is generally directed to the vegetative propagation techniques such as traditional Fructus actinidiae chinensis cuttage, grafting, growing-seedling period is long, and germination percentage is low
Technological deficiency；On the other hand, based on the vegetative technological improvement such as cuttage, grafting, often appropriate only to single
Kiwifruit Cultivars, does not have general applicability, it is difficult to the state of the art of popularization and application between different Kiwifruit Cultivars, proposes one
Plant the method for quickly breeding going for multiple Fructus actinidiae chinensis species, meet the requirement of factorial praluction.
For solving the technical problem pointed by the present invention, the present invention, on the basis of great many of experiments is explored, summarizes one
It is applicable to the method for quickly breeding of multiple Kiwifruit Cultivars.The detailed description of the invention of described method for quickly breeding, including following step
Rapid:
A kind of method for quickly breeding being applicable to multiple Kiwifruit Cultivars, including following step:
(1) preparation of propagating materials
The branch that clip is raw in 2 years from selected Fructus actinidiae chinensis maternal plant, is cut into the branch section of every root length degree about 10cm by branch,
Branch section base portion sharp knife is whittled into the angle of about 45 degree, and branch section top is whittled into flat mouth, cuts off at branch section base portion 4-5cm
Leaf, retains 2-3 sheet leaf, as cottage propagation material at branch section top；
(2) immersion of propagating materials
Ready branches for cuttage section is quickly immersed immersion 20-30min in the ABT rooting powder solution of 100mg/L concentration；
(3) accelerating germination of propagating materials
The triangular flask that the selection of accelerating germination container cleans up, boiled water is disinfected, fills tap water or pure water in each triangular flask
50ml, after the branch section after soaking inserts triangular flask, is positioned over illumination box；Humidity in illumination box remains
80%, illumination every day 12h, intensity of illumination is not less than 2000Lx, and during illumination, the temperature in illumination box remains 25 DEG C；Often
It dark processing 12h, the temperature in illumination box remains 25 DEG C during dark processing；
(4) propagating materials urge root
Urge root at branch section the 4-5 days, the blade spraying foliage fertilizer retained to branch section, and by the tap water in triangular flask or
Pure water is outwelled, and is replaced as new tap water or pure water；
(5) point cultivation of propagating materials
Branch section processes 10-12 days, when branch section base portion bears the root about length 0.5cm, by branch section from three through urging root
Angle bottle is transplanted to the container being loaded with turfy soil；
(6) seedling exercising
In point cultivation branch section container, water spray makes the humidity of turfy soil be maintained at about 80%, and temperature about 25 DEG C, illumination is strong
Seedling exercising 20-30 days in the environment of degree 3000-5000Lx.
Preferably, described be applicable to the method for quickly breeding of multiple Kiwifruit Cultivars, described foliage fertilizer is a kind of big
Secondary element water-soluble fertilizer, a great number of elements N+P2O5+K2O >=58.0%, trace element Mg >=1.2%, concentration is tap water or pure
1000-1500 times of water purification consumption.
Preferably, described be applicable to the method for quickly breeding of multiple Kiwifruit Cultivars, described Fructus actinidiae chinensis maternal plant is preferred
For Actinidia arguta Sieb.et Zucc, Rhesus Monkey in Shaanxi Province Fructus Persicae, black stamen Fructus actinidiae chinensis maternal plant.
With tradition cuttage technique compared with, beneficial effects of the present invention or advantage major embodiment in the following areas:
1, cuttage is being introduced a fine variety as the technology of a kind of pierre and is extensively being applied in expanding propagation.Fast-propagation side of the present invention
Method is different from traditional cottage method, fully achieves Actinidia arguta Sieb.et Zucc, Rhesus Monkey in Shaanxi Province Fructus Persicae, the batch production of black stamen Fructus actinidiae chinensis are educated
Seedling, from the preparation of propagating materials to point cultivation seedling exercising achieved with success, and shortens the production cycle, eliminates tissue
Cultivate or the high cost step such as culture medium configuration, the accelerating germination of propagating materials and to urge the root stage be all to be immersed in tap water or pure water
In, it is greatly saved production cost.
2, the whole breeding operation used time is short, tests through expanding propagation repeatedly and knows, uses method for quickly breeding of the present invention,
General time only needing about 15 days, preferably 10-12 days, Actinidia arguta Sieb.et Zucc, Rhesus Monkey in Shaanxi Province Fructus Persicae, black stamen Fructus actinidiae chinensis branch section
Germination rooting rate up to 95% and more than, and growth of seedling is healthy and strong.
3, method for quickly breeding of the present invention is easily mastered, and program is simple, practical, the workman in nursery workshop
It is prone to grasp and operation.Foliage fertilizer and root-inducing powder etc. used in the present invention, to Actinidia arguta Sieb.et Zucc, Rhesus Monkey in Shaanxi Province Fructus Persicae, black stamen macaque
The expanding propagation of Fructus Persicae has significant facilitation, and the preparation of described foliage fertilizer and root-inducing powder is cheap, draw materials conveniently, significantly
Improve practical value and the productive value of the present invention.
4, divide cultivation substrate only with turfy soil, also reduce the cost of substrate.Meanwhile, the present invention is recommended
Being the most lignified newborn branch, this is the key choosing propagating materials.The most lignified newborn branch, it is easy on maternal plant
Choose and identification.
5, method for quickly breeding of the present invention is not merely for the expanding propagation of single Kiwifruit Cultivars, but passes through
The most long-term testing sieve have selected and is applicable to Actinidia arguta Sieb.et Zucc, Rhesus Monkey in Shaanxi Province Fructus Persicae, the universal method of black stamen Kiwifruit Cultivars,
The more operation of laminating nursery factory is actual.The method for quickly breeding of universality, a fado use, reduce the equipment of nursery factory
And material cost, also reduce the training cost of workman simultaneously, improve the overall efficiency of nursery factory.
Below with reference to embodiment, the present invention is elaborated further.
Detailed description of the invention
Embodiment 1
The present embodiment provides a kind of method for quickly breeding being applicable to Actinidia arguta Sieb.et Zucc kind, including following step:
(1) preparation of propagating materials
The branch that clip is raw in 2 years from Actinidia arguta Sieb.et Zucc maternal plant, the newborn branch departing from maternal plant should carry out follow-up place as early as possible
Reason, it is to avoid newborn branch exposes the long time in the sun, prevents newborn branch from occurring because of dehydration wilting.
Newborn branch shears is cut into the branch section of every root length degree about 10cm, and in practical operation, branch section length can
To control in the range of 8cm-12cm, keep length consistent, follow-up industrial seedling rearing as far as possible.The base portion of every branch section
Sharp knife is whittled into the angle of about 45 degree, and in practical operation, the gradient of angle should control in the range of 40-50 degree, keeps as far as possible
The angle gradient is consistent, and beneficially branch section absorbs moisture, and the germination keeping neat is taken root.
Branch section top is whittled into or is cut into flat mouth, and otch keeps smooth as far as possible, cuts off the leaf at branch section base portion 4-5cm
Son, retains 2-3 sheet leaf, completes the preparation of cottage propagation material at branch section top.
(2) immersion of propagating materials
Ready branches for cuttage section is quickly immersed immersion 20-30min in the ABT rooting powder solution of 100mg/L concentration.This
ABT root-inducing powder described in step is purchased from ABT research and development centre of the Chinese Academy of Forestry.ABT rooting powder solution used
Concentration is difficult to too high, it is to avoid branch section enters in the ABT rooting powder solution of high concentration.Preferably, ABT rooting powder solution concentration is
80mg/L-150mg/L scope, is all acceptable, and meets the requirement of the method for the invention.
(3) accelerating germination of propagating materials
This step requires the vessel that the selection of accelerating germination container cleans up, boiled water was disinfected, it is preferable that the triangle of glass material
Bottle can be as accelerating germination container, the accelerating germination container that Ye Shi nursery factory is standing.Each triangular flask after sterilization fills tap water
50ml, if if conditions permit, it is possible to use pure water, or high purity water.It preferably is from water or pure water, mainly considers
With the convenience of water and control cost.
After branch section after soaking inserts triangular flask, it is positioned over illumination box.Humidity in illumination box keeps
Being 80%, in practical operation, humidity remains 70%-85% and is possible to the present invention's.Illumination every day 12h, intensity of illumination is not
Less than 2000Lx, intensity of illumination is preferably 3000 Lx, and during illumination, the temperature in illumination box remains 25 DEG C；Every darkness
Dark treatment 12h, the temperature in illumination box remains 25 DEG C during dark processing.Temperature in incubator controls at 20-35
It it is all acceptable in the range of DEG C.
(4) propagating materials urge root
Urge root at branch section the 4-5 days, the blade spraying foliage fertilizer retained to branch section, and by the tap water in triangular flask or
Pure water is outwelled, and is replaced as new tap water or pure water.Change the purpose of water, be to prevent the tap water in triangular flask or pure water
Long period stands generation and goes mouldy, and is unfavorable for rooting of branch section.Typically urge the 4th day of root at branch section that to change water the suitableeest
Preferably.
The foliage fertilizer that this step uses is a kind of a great number of elements water-soluble fertilizer, and the key technical indexes is N+P2O5+K2O≥
58.0%, Mg >=1.2%, the concentration of 1000-1500 times.
(5) point cultivation of propagating materials
Branch section processes 10-12 days, when branch section base portion bears the root about length 0.5cm, by branch section from three through urging root
Angle bottle is transplanted to the container being loaded with turfy soil.Urge root process the time should depending on the growing state of the new root of branch section base portion,
Needing periodically to check, the new root length degree controlling branch section base portion is not to be exceeded 1cm, and when preventing from point planting, young root damages.
(6) seedling exercising
In point cultivation branch section container, water spray makes the humidity of turfy soil be maintained at about 80%, it is preferable that the humidity of turfy soil is protected
Hold 70%-85% to be all satisfactory.Seedling exercising temperature about 25 DEG C, intensity of illumination keeps seedling exercising in the environment of 3000-5000Lx
20 days.
Embodiment 2
The present embodiment provides a kind of method for quickly breeding being applicable to Rhesus Monkey in Shaanxi Province Peach cultivars, including following step:
(1) preparation of propagating materials
The branch that clip is raw in 2 years from Rhesus Monkey in Shaanxi Province Fructus Persicae maternal plant, the branch departing from maternal plant should carry out follow-up process as early as possible, keep away
Exempt from newborn branch and expose the long time in the sun, prevent newborn branch from occurring because of dehydration wilting.
Newborn branch shears is cut into the branch section of every root length degree about 10cm, and in practical operation, branch section length can
To control in the range of 8cm-12cm, keep length consistent, follow-up industrial seedling rearing as far as possible.The base portion of every branch section
Sharp knife is whittled into the angle of about 45 degree, and in practical operation, the gradient of angle should control in the range of 40-50 degree, keeps as far as possible
The angle gradient is consistent, and beneficially branch section absorbs moisture, and the germination keeping neat is taken root.
Branch section top is whittled into or is cut into flat mouth, and otch keeps smooth as far as possible, cuts off the leaf at branch section base portion 4-5cm
Son, retains 2-3 sheet leaf, completes the preparation of cottage propagation material at branch section top.
(2) immersion of propagating materials
Ready branches for cuttage section is quickly immersed immersion 20-30min in the ABT rooting powder solution of 100mg/L concentration.This
ABT root-inducing powder described in step is purchased from ABT research and development centre of the Chinese Academy of Forestry.ABT rooting powder solution used
Concentration is difficult to too high, it is to avoid branch section enters in the ABT rooting powder solution of high concentration.Preferably, ABT rooting powder solution concentration is
80mg/L-150mg/L scope, is all acceptable, and meets the requirement of the method for the invention.
(3) accelerating germination of propagating materials
This step requires the vessel that the selection of accelerating germination container cleans up, boiled water was disinfected, it is preferable that the triangle of glass material
Bottle can be as accelerating germination container, the accelerating germination container that Ye Shi nursery factory is standing.Each triangular flask after sterilization fills tap water
50ml, if if conditions permit, it is possible to use pure water, or high purity water.It preferably is from water or pure water, mainly considers
With the convenience of water and control cost.
After branch section after soaking inserts triangular flask, it is positioned over illumination box.Humidity in illumination box keeps
Being 80%, in practical operation, humidity remains 70%-85% and is possible to the present invention's.Illumination every day 12h, intensity of illumination is not
Less than 2000Lx, intensity of illumination is preferably 3000 Lx, and during illumination, the temperature in illumination box remains 25 DEG C；Every darkness
Dark treatment 12h, the temperature in illumination box remains 25 DEG C during dark processing.Temperature in incubator controls at 22-28
It it is all acceptable in the range of DEG C.
(4) propagating materials urge root
Urge root at branch section the 4-5 days, the blade spraying foliage fertilizer retained to branch section, and by the tap water in triangular flask or
Pure water is outwelled, and is replaced as new tap water or pure water.Change the purpose of water, be to prevent the tap water in triangular flask or pure water
Long period stands generation and goes mouldy, and is unfavorable for rooting of branch section.Typically urge the 4th day of root at branch section that to change water the suitableeest
Preferably.
The foliage fertilizer that this step uses is a kind of a great number of elements water-soluble fertilizer, and the key technical indexes is N+P2O5+K2O≥
58.0%, Mg >=1.2%, the concentration of 1000-1500 times.
(5) point cultivation of propagating materials
Branch section processes 10-12 days, when branch section base portion bears the root about length 0.5cm, by branch section from three through urging root
Angle bottle is transplanted to the container being loaded with turfy soil.Urge root process the time should depending on the growing state of the new root of branch section base portion,
Needing periodically to check, the new root length degree controlling branch section base portion is not to be exceeded 1cm, and when preventing from point planting, young root damages.
(6) seedling exercising
In point cultivation branch section container, water spray makes the humidity of turfy soil be maintained at about 80%, it is preferable that the humidity of turfy soil is protected
Hold 70%-85% to be all satisfactory.Seedling exercising temperature about 25 DEG C, intensity of illumination keeps seedling exercising in the environment of 3000-5000Lx
25 days.
Embodiment 3
The present embodiment provides a kind of method for quickly breeding being applicable to black stamen Kiwifruit Cultivars, including following step:
(1) preparation of propagating materials
The branch that clip is raw in 2 years from black stamen Fructus actinidiae chinensis maternal plant, the newborn branch departing from maternal plant should carry out follow-up place as early as possible
Reason, it is to avoid newborn branch exposes the long time in the sun, prevents newborn branch from occurring because of dehydration wilting.
Newborn branch shears is cut into the branch section of every root length degree about 10cm, and in practical operation, branch section length can
To control in the range of 8cm-12cm, keep length consistent, follow-up industrial seedling rearing as far as possible.The base portion of every branch section
Sharp knife is whittled into the angle of about 45 degree, and in practical operation, the gradient of angle should control in the range of 40-50 degree, keeps as far as possible
The angle gradient is consistent, and beneficially branch section absorbs moisture, and the germination keeping neat is taken root.
Branch section top is whittled into or is cut into flat mouth, and otch keeps smooth as far as possible, cuts off the leaf at branch section base portion 4-5cm
Son, retains 2-3 sheet leaf, completes the preparation of cottage propagation material at branch section top.
(2) immersion of propagating materials
Ready branches for cuttage section is quickly immersed immersion 20-30min in the ABT rooting powder solution of 100mg/L concentration.This
ABT root-inducing powder described in step is purchased from ABT research and development centre of the Chinese Academy of Forestry.ABT rooting powder solution used
Concentration is difficult to too high, it is to avoid branch section enters in the ABT rooting powder solution of high concentration.Preferably, ABT rooting powder solution concentration is
80mg/L-150mg/L scope, is all acceptable, and meets the requirement of the method for the invention.
(3) accelerating germination of propagating materials
This step requires the vessel that the selection of accelerating germination container cleans up, boiled water was disinfected, it is preferable that the triangle of glass material
Bottle can be as accelerating germination container, the accelerating germination container that Ye Shi nursery factory is standing.Each triangular flask after sterilization fills tap water
50ml, if if conditions permit, it is possible to use pure water, or high purity water.It preferably is from water or pure water, mainly considers
With the convenience of water and control cost.
After branch section after soaking inserts triangular flask, it is positioned over illumination box.Humidity in illumination box keeps
Being 80%, in practical operation, humidity remains 70%-85% and is possible to the present invention's.Illumination every day 12h, intensity of illumination is not
Less than 2000Lx, intensity of illumination is preferably 3000 Lx, and during illumination, the temperature in illumination box remains 25 DEG C；Every darkness
Dark treatment 12h, the temperature in illumination box remains 25 DEG C during dark processing.Temperature in incubator controls at 20-35
It it is all acceptable in the range of DEG C.
(4) propagating materials urge root
Urge root at branch section the 4-5 days, the blade spraying foliage fertilizer retained to branch section, and by the tap water in triangular flask or
Pure water is outwelled, and is replaced as new tap water or pure water.Change the purpose of water, be to prevent the tap water in triangular flask or pure water
Long period stands generation and goes mouldy, and is unfavorable for rooting of branch section.Typically urge the 4th day of root at branch section that to change water the suitableeest
Preferably.
The foliage fertilizer that this step uses is a kind of a great number of elements water-soluble fertilizer, and the key technical indexes is N+P2O5+K2O≥
58.0%, Mg >=1.2%, the concentration of 1000-1500 times.
(5) point cultivation of propagating materials
Branch section processes 10-12 days, when branch section base portion bears the root about length 0.5cm, by branch section from three through urging root
Angle bottle is transplanted to the container being loaded with turfy soil.Urge root process the time should depending on the growing state of the new root of branch section base portion,
Needing periodically to check, the new root length degree controlling branch section base portion is not to be exceeded 1cm, and when preventing from point planting, young root damages.
(6) seedling exercising
In point cultivation branch section container, water spray makes the humidity of turfy soil be maintained at about 80%, it is preferable that the humidity of turfy soil is protected
Hold 70%-85% to be all satisfactory.Seedling exercising temperature about 25 DEG C, intensity of illumination keeps seedling exercising in the environment of 3000-5000Lx
30 days.
Embodiment 4
In order to those skilled in the art represent method for quickly breeding of the present invention (hereinafter referred to as the inventive method) and
Its effect, the present embodiment provides the inventive method and the contrast test of 70% light spraying cottage method.Described in the present embodiment 70%
Light spraying cottage method, mainly includes following operating procedure:
(1) preparation in cuttage seedbed
This step requires river sand cuttage seedbed.River sand is sterilized by the carbendazim of 500 times, uses atomizer spray tap water.Insert
30% shade net taken by bed.
(2) preparation of propagating materials
The branch that clip is raw in 2 years from Fructus actinidiae chinensis maternal plant, is cut into the branch section of every root length degree about 10cm by branch.Every branch
The base portion sharp knife of bar section is whittled into the angle of about 45 degree, and branch section top is cut into flat mouth, and otch keeps smooth as far as possible, has leaf period
Cut off the leaf at branch section base portion 4-5cm, retain 1-2 sheet leaf at branch section top, complete the preparation of cottage propagation material
Work.
(3) immersion of propagating materials
Ready branches for cuttage section is quickly immersed immersion 20-30min in the ABT rooting powder solution of 100mg/L concentration.
(4) propagating materials urge root
Cuttage branch is inserted and is buried branch half.Morning 8 to spraying at 8 in evening, except rainy day.
(5) point cultivation of propagating materials
Branch section is through urging root to process after 20-25 days, when branch section base portion bears the root about length 0.5cm, by branch section from
Triangular flask is transplanted to the container being loaded with turfy soil.
(6) seedling exercising
The humidity of turfy soil is made to be maintained at about 80% to point planting water spray in branch section container, seedling exercising temperature about 25 DEG C, 30%
Seedling exercising 30 days in the environment of sheltering from heat or light.
Material to be tested is Actinidia arguta Sieb.et Zucc, Rhesus Monkey in Shaanxi Province Fructus Persicae, the raw branch of black stamen Fructus actinidiae chinensis 1-2, it is desirable to the branch of institute's clip
The length of bar section, thickness and growth the age basically identical, and start test before, the branch section of each Kiwifruit Cultivars with
Machine is chosen, and eliminates the impact of personal error in experimentation as far as possible.Each Kiwifruit Cultivars respectively arranges one group of contrast real
Testing, often group experiment repeats for 3 times, takes the meansigma methods index as a comparison of the rooting rate repeated 3 times.Experimental result after statistics is shown in Table
1。
The table 1 different disposal method experimental result to selected Kiwifruit Cultivars rooting rate
As it can be seen from table 1 compared to 70% light spraying cottage method, the rooting rate of the inventive method has all exceeded 95%, and
The survival rate in seedling exercising stage, to about 90%, well highlights the beneficial effect being applicable to rapid propagation in factory.Additionally,
The rootage duration of the inventive method significantly shortens, and normally only needs 15 days, can obtain preferable rooting rate, and this point is also 70%
Light spraying cottage method is incomparable.Furthermore, the inventive method is not limited by Kiwi growth season, in the range of the whole year
The most applicable, this is also different from the another remarkable advantage of 70% light spraying cottage method.
Above in conjunction with embodiment, the present invention done further narration, but the present invention has been not limited to above-mentioned embodiment,
In the ken that one skilled in the relevant art is possessed, it is also possible to make on the premise of without departing from present inventive concept
Various changes.
Claims (3)
1. the method for quickly breeding being applicable to multiple Kiwifruit Cultivars, it is characterised in that include following step:
(1) preparation of propagating materials
The branch that clip is raw in 2 years from selected Fructus actinidiae chinensis maternal plant, is cut into the branch section of every root length degree about 10cm by branch,
Branch section base portion sharp knife is whittled into the angle of about 45 degree, and branch section top is whittled into flat mouth, cuts off at branch section base portion 4-5cm
Leaf, retains 2-3 sheet leaf, as cottage propagation material at branch section top；
(2) propagating materials is soaked
Ready branches for cuttage section is quickly immersed immersion 20-30min in the ABT rooting powder solution of 100mg/L concentration；
(3) accelerating germination of propagating materials
The triangular flask that the selection of accelerating germination container cleans up, boiled water is disinfected, fills tap water or pure water in each triangular flask
50ml, after the branch section after soaking inserts triangular flask, can insert 1-2 root branch section, be positioned over illumination box for every bottle；Illumination is trained
The humidity supported in case remains 80%, and illumination every day 12h, intensity of illumination is not less than 2000Lx, during illumination in illumination box
Temperature remains 25 DEG C；Every day dark processing 12h, the temperature in illumination box remains 25 DEG C during dark processing；
(4) propagating materials urge root
Urge root at branch section the 4-5 days, the blade spraying foliage fertilizer retained to branch section, and by the tap water in triangular flask or
Pure water is outwelled, and is replaced as new tap water or pure water；
(5) point cultivation of propagating materials
Branch section processes 10-12 days, when branch section base portion bears the root about length 0.5cm, by branch section from three through urging root
Angle bottle is transplanted to the container being loaded with turfy soil；
(6) seedling exercising
In point cultivation branch section container, water spray makes the humidity of turfy soil be maintained at about 80%, and temperature about 25 DEG C, illumination is strong
Seedling exercising 20-30 days in the environment of degree 3000-5000Lx.
The method for quickly breeding being applicable to multiple Kiwifruit Cultivars the most according to claim 1, it is characterised in that described leaf
Leaven is a kind of a great number of elements water-soluble fertilizer, a great number of elements N+P2O5+K2O >=58.0%, trace element Mg >=1.2%, concentration
For tap water or 1000-1500 times of pure water consumption.
The method for quickly breeding being applicable to multiple Kiwifruit Cultivars the most according to claim 1, it is characterised in that described choosing
Fixed Fructus actinidiae chinensis maternal plant is Actinidia arguta Sieb.et Zucc, Rhesus Monkey in Shaanxi Province Fructus Persicae, black stamen Fructus actinidiae chinensis maternal plant.
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| CN109429853A (en) * | 2018-10-12 | 2019-03-08 | 四川省益诺仕农业科技有限公司 | A kind of Propagation of Actinidia Arguta By Green Cutting method |
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| CN111149701A (en) * | 2020-02-27 | 2020-05-15 | 通化师范学院 | Rapid seedling propagation method for actinidia arguta |
| CN111771553A (en) * | 2020-07-31 | 2020-10-16 | 中国农业科学院郑州果树研究所 | Method for raising seedlings of actinidia valvata by cutting |
| CN114342676A (en) * | 2020-10-13 | 2022-04-15 | 丹东农业科学院 | Fast seedling method for actinidia arguta hard branch cuttage |
| CN112470730A (en) * | 2020-12-07 | 2021-03-12 | 中国科学院武汉植物园 | Asexual cuttage propagation method for actinidia arguta |
| CN114885688A (en) * | 2022-04-25 | 2022-08-12 | 西北农林科技大学 | Kiwi fruit micro-root grafting and cuttage method |
| CN114885688B (en) * | 2022-04-25 | 2024-04-02 | 西北农林科技大学 | Kiwi fruit micro-root grafting cutting method |
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