CN103869058A - Rapid evaluation method for salt tolerance of tomatoes - Google Patents
Rapid evaluation method for salt tolerance of tomatoes Download PDFInfo
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- CN103869058A CN103869058A CN201410107889.3A CN201410107889A CN103869058A CN 103869058 A CN103869058 A CN 103869058A CN 201410107889 A CN201410107889 A CN 201410107889A CN 103869058 A CN103869058 A CN 103869058A
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Abstract
The invention discloses a rapid evaluation method for the salt tolerance of tomatoes. The rapid evaluation method is characterized in that wild tomatoes with very high salt tolerance, namely solanum pennellii LA716, are adopted as a reference substance, and the salt tolerance degree of a tomato material to be evaluated is compared with that of the solanum pennellii LA716 under different concentrations of artificial seawater for making quick judgment. The rapid evaluation method comprises the specific steps of: (1) planting the solanum pennellii LA716 and the tomato material to be evaluated; (2) carrying out tissue cultivation or water cultivation on the solanum pennellii LA716 and the tomato material to be evaluated; (3) carrying out a salt tolerance gradient experiment on the solanum pennellii LA716 and the tomato material to be evaluated; and (4) comparing and evaluating the salt tolerance conditions of the tomato material to be evaluated and the solanum pennellii LA716 so as to directly judge the salt tolerance sequence of various tomato materials. The rapid evaluation method for the salt tolerance of the tomatoes is simple and convenient, is easy to grasp and is suitable for rapidly identifying the salt tolerance degrees of tomato varieties; the rapid evaluation method is used for culture experiments and production to obtain high-output and high-quality products and the maximum benefit is obtained.
Description
Technical field
The present invention relates to a kind of fast appraisement method of tomato salt-tolerant degree, can be used for the salt tolerant breeding of tomato, the plantation popularization of salt tolerant kind, belong to crop breeding technical field.
Background technology
Tissue culture technique is mature technology conventional in crop breeding, carries out axenic cultivation and obtain the plant of whole strain by cutting the plant parts such as leaf, stem, root.Water culture technique is the power of regeneration of utilizing plant, chooses blade, branch, is immersed in sterilized water and takes root, and promotes the growth of plant by injecting nutrient solution.
Saliferous soil distributes very wide in the world, area account for the world land total area 1/3rd (Zhai Fenglin, Cao Mingqing. the brine tolerance of plant and improvement thereof. Beijing: agriculture publishing house, 1989,3-4).Salinity is one of essential environmental factors of limiting plant growth and output (Genin S, Boucher C.2002).Cultivate and plantation salt-tolerant plant is that the mankind overcome one of important channel that the soil salinization and fresh water lacks.Tomato (Lycopersicon esculentum Mill), belongs to Solanaceae tomato and belongs to, and is that a kind of moderate salt responsive type is eaten raw and processing type vegetable crop, and nutritive value is very high, is the important vegetable of many countries.In recent years, the cultivated area of tomato constantly expands in the whole world, and many countries have all carried out the research of salt tolerant tomato, makes full use of the newly-increased arable land of reclaiming land from the sea and expands tomato planting area or be used for overcoming the soil salinization producing under Protected Cultivation.Wherein, Pan Nali tomato (Solanum pennellii LA716) is one of the Collections of Wild Tomato Species Lycopersicon of salt tolerant (Jones R A.High salt tolerance potential in Lyeopersion species during germinateon.Euphytica, 1986,35:575-582), be used as for a long time Salt-tolerant Materials research.
Tomato is a kind of strict self-pollinated plant, is easier to operation at ratio aspect breeding subculture purifying, in recent years by mixing breed, resistant gene of salt import, the method seed selection such as Natural Selection wide in variety has report under hypersaline environment.We have also been bred as three salt tolerant kinds in laboratory.But in prior art, the method that tomato salt-tolerant is evaluated is mainly passed through tomato salt-tolerant index, for example, at physical signs (Li Ye such as salt damage exponential sum chlorophyll content, membrane permeabilities, Jiang Jingbin, Deng. the screening study of tomato seedling brine tolerance index of correlation. northern gardening, 2009(10): 84-86) study, but these indexs are difficult to be applied in actual production.
Summary of the invention
The present invention is directed at present tomato salt-tolerant degree without effective authentication method, in popularization, lack the present situation of foundation, develop a kind of fast appraisement method of tomato salt-tolerant degree, make breeder, researchist and the producer can understand intuitively the salt tolerant degree of different cultivars, select thereby make kind.
The present invention is using the extremely strong Pan Nali tomato of brine tolerance (Solanum pennellii LA716) as reference; Be optimized proportioning based on Mocledon artificial seawater, remove the compound of content lower than 0.5g/L, and artificial seawater concentration gradient is set; Adopt two kinds of different plant establishment modes to test.The present invention is achieved through the following technical solutions, and concrete steps are as follows:
(1) plantation of Pan Nali tomato and tomato material to be evaluated;
(2) tissue of Pan Nali tomato and tomato material to be evaluated is cultivated or water planting;
(3) the salt tolerant gradient of Pan Nali tomato and tomato material to be evaluated experiment;
(4) the salt tolerant situation of tomato material to be evaluated and comparison and the evaluation of Pan Nali tomato; Wherein:
In step (3), the concrete grammar of the salt tolerant gradient experiment of Pan Nali tomato and tomato material to be evaluated is: by CaCl
21.153g, MgCl
22.26g, MgSO
43.248g, KCl0.721g, NaCl26.726g are dissolved in distilled water, and constant volume to 1 liter, concentration artificial seawater is helped in preparation, use again distilled water diluting, be divided into 5-10 different gradients the two leaves plant wholeheartedly that grows into of Pan Nali tomato and tomato material to be evaluated is placed in each gradient artificial seawater and is cultivated.
In step (1), the concrete grammar that the plantation of described Pan Nali tomato and tomato material to be evaluated is taked is: the planting seed of Pan Nali tomato and tomato material to be evaluated, in the matrix of cave dish, is obtained to the plantling growing fine.
In step (2), the tissue of described Pan Nali tomato and tomato material to be evaluated is cultivated the concrete grammar of taking: clip increment identical with two leaves Pan Nali tomato wholeheartedly and the side shoot with blade of tomato material to be evaluated, after sterilization, under aseptic technique, be transplanted on the 1/2MS nutrient culture media of sterilizing and carry out culture of rootage.
In step (2), the concrete grammar that described Pan Nali tomato is taked with the water planting of tomato material to be evaluated is: clip increment identical with Pan Nali tomato wholeheartedly of two leaves and tomato material to be evaluated side shoot, after wound disinfection, be placed on and in sterilized water, carry out culture of rootage.
In step (4), the salt tolerant situation of described tomato material to be evaluated from the concrete grammar that comparison and the evaluation of Pan Nali tomato taked is: the growth and mortality situation in different gradient artificial seawaters according to Pan Nali tomato and tomato material to be evaluated, each group experiment material is compared and evaluated, determine the tolerance of salinity of tomato material to be evaluated.
In the present invention, taking Pan Nali tomato as contrast, tomato to be evaluated is research material, train and ciltivating process by group, obtain and be grown to two leaves tomato material wholeheartedly, under isometric growth condition, be placed on respectively in the salt solution of different gradients and cultivate, compare depending on its salt tolerant situation and Pan Nali tomato, evaluate its tolerance of salinity.The expression mode of salt tolerant degree is part Pan Nali tomato salt-tolerant degree, and more salt tolerant degree is lower for denominator.Can distinguish the salt tolerant degree of various tomatoes with part Pan Nali tomato salt-tolerant degree, for the salt tolerant kind in actual production selects to provide foundation.
Beneficial effect of the present invention is: research relatively in the past, more intuitively the salt tolerant New Tomato Variety of new cultivation and introduction is carried out to qualitative description, being convenient to vegetable grower carries out in process of production the different tomato seeds of choice for use salt tolerant degree and is planted in applicable soil, the product that obtains high yield, high-quality, obtains maximum return.
Embodiment
Below embodiments of the invention are elaborated: the present embodiment is implemented under taking technical solution of the present invention as prerequisite, provided detailed embodiment and detailed process, but protection scope of the present invention is not limited to following embodiment.
All vegetable materials that table 1 the present invention is used
Embodiment 1
(1) material plantation
Choose full Pan Nali tomato (from gardening portion of California, USA university) and hand over the seed of the tomato varieties such as No. 1, agriculture, No. 2, agriculture of friendship, " fresh rich " to carry out vernalization, by the seed sowing of germination in peat is housed: perlite: sand=1: in the nutritive cube of 1: 2, be placed in double-layer inflatable film greenhouse and grow seedlings.After the plant branch out of Pan Nali tomato and other tomatoes, the side shoot with blade that clip thickness is consistent, length is the same is for taking root.
(2) group training is taken root
The tomato branch for taking root, through sterilization, under aseptic condition, cuttage is on 1/2MS nutrient culture media, and in every 100gMS nutrient culture media, containing sucrose 3.0g, agar 0.8g, pH5.8, is that 25 1 DEG C, soil, light intensity are 25 μ molm in temperature
-2s
-1, the photoperiod is to cultivate under the condition that 14h light/10h is dark.After branch bears root, carry out seawater culture experiment.
(3) preparation of artificial seawater and nutrient solution and gradient experiment
The configuration of artificial seawater and nutrient solution: every liter of artificial seawater concentration is containing CaCl
21.153g, MgCl
22.26g, MgSO
43.248g, KCl0.721g, NaCl26.726g; Hoagland nutrient solution (four water-calcium nitrate 945mg/L, potassium nitrate 506mg/L, ammonium nitrate 80mg/L, potassium dihydrogen phosphate 136mg/L, magnesium sulfate 493mg/L, iron salt solutions 2.5ml, liquid microelement 5ml, pH=6.0).
Gradient experimental program: point 7 processing: (1) is only to water 1/2 concentration Hoagland nutrient solution (CK) in contrast; (2) process 1 (ST1) with carrying out salt containing half Hoagland nutrient solution of 1/5 artificial seawater salinity; (3) process 2 (ST2) with carrying out salt containing half Hoagland nutrient solution of 1/4 artificial seawater salinity; (4) process 3 (ST3) with carrying out salt containing half Hoagland nutrient solution of 1/3 artificial seawater salinity; (5) process 4 (ST4) with carrying out salt containing half Hoagland nutrient solution of 1/2 artificial seawater salinity; (6) process 5 (ST5) with carrying out salt containing half Hoagland nutrient solution of 2/3 artificial seawater salinity; (7) process 6 (ST6) with carrying out salt containing half Hoagland nutrient solution of 4/5 artificial seawater salinity; .
With above gradient experimental solutions preparation MS nutrient culture media, the tomato branch that transplanting has been taken root under aseptic condition, the growing state of the each tomato variety of observed and recorded, determine the tolerance of salinity of each tomato variety.
(4) results and analysis
Result is as table 2:
Table 2 tomato variety salt tolerant summary sheet
| ? | CK | ST1 | ST2 | ST3 | ST4 | ST5 | ST6 |
| Pan Nali tomato | Normally | Normally | Normally | Normally | Normally | Normally | There is wilting |
| Hand over No. 1, agriculture | Normally | Normally | Normally | Normally | There is wilting | Dead | ? |
| Hand over No. 2, agriculture | Normally | Normally | Normally | There is wilting | Dead | ? | ? |
| Fresh rich | Normally | There is wilting | Dead | ? | ? | ? | ? |
Along with the increase of concentration of seawater, in identical incubation time, " fresh rich " tomato variety ST2 death, hands over No. 2 ST4 death of agriculture, hands over No. 1 ST5 death of agriculture, and Pan Nali tomato has wilting at ST6.Can determine by comparing the brine concentration adding in nutrient culture media the salt tolerant degree of these four tomato varieties, hand over No. 1, agriculture: Pan Nali tomato=2/3 artificial seawater salinity: 4/5 artificial seawater salinity=5/6 Pan Nali tomato salt-tolerant degree; Hand over No. 2, agriculture: Pan Nali tomato=1/2 artificial seawater salinity: 4/5 artificial seawater salinity=5/8 Pan Nali tomato salt-tolerant degree; " fresh rich ": Pan Nali tomato=1/4 artificial seawater salinity: 4/5 artificial seawater salinity=5/16 Pan Nali tomato salt-tolerant degree.
(5) conclusion
Handing over No. 1 tolerance of salinity of agriculture is 5/6 of Pan Nali tomato, handing over No. 2 tolerance of salinitys of agriculture is 5/8 of Pan Nali tomato, " fresh rich " tomato salt-tolerant degree is 5/16 of Pan Nali tomato, using Pan Nali tomato as object of reference, just can judge very intuitively the salt tolerant degree of each tomato variety.In the present embodiment, tolerance of salinity order is: hand over No. 1 > of agriculture to hand over No. 2 > of agriculture " fresh rich " tomato.
Embodiment 2
(1) material plantation
Choose No. 1, full Pan Nali tomato and Jiao Nong, hand over the seed of the tomato varieties such as No. 2, agriculture, " fresh rich " to carry out vernalization, by the seed sowing of germination in peat is housed: perlite: sand=1: in the nutritive cube of 1: 2, be placed in double-layer inflatable film greenhouse and grow seedlings.After the plant branch out of Pan Nali tomato and other tomatoes, the side shoot that clip thickness is consistent, length is the same is used for taking root.
(2) water planting is taken root
Distilled water is carried out disinfection, is placed in wide-necked bottle, add appropriate Hoagland nutrient solution, then the tomato branch for taking root after sterilization, put into wide-necked bottle, a bottom axil will submerge in water, carries out culture of rootage under low light condition.After branch bears root, carry out seawater culture experiment.
(3) preparation of artificial seawater and nutrient solution and gradient experiment
The configuration of artificial seawater and nutrient solution: every liter of artificial seawater concentration is containing CaCl
21.153g, MgCl
22.26g, MgSO
43.248g, KCl0.721g, NaCl26.726g; Hoagland nutrient solution (four water-calcium nitrate 945mg/L, potassium nitrate 506mg/L, ammonium nitrate 80mg/L, potassium dihydrogen phosphate 136mg/L, magnesium sulfate 493mg/L, iron salt solutions 2.5ml, liquid microelement 5ml, pH=6.0).
Gradient experimental program: point 3 processing: (1) is only to water 1/2 concentration Hoagland nutrient solution (CK) in contrast; (2) process 1 (ST1) with carrying out salt containing half Hoagland nutrient solution of 1/5 artificial seawater salinity; (3) process 2 (ST2) with carrying out salt containing half Hoagland nutrient solution of 1/4 artificial seawater salinity; (4) process 3 (ST3) with carrying out salt containing half Hoagland nutrient solution of 1/3 artificial seawater salinity; (5) process 4 (ST4) with carrying out salt containing half Hoagland nutrient solution of 1/2 artificial seawater salinity; (6) process 5 (ST5) with carrying out salt containing half Hoagland nutrient solution of 2/3 artificial seawater salinity; (7) process 6 (ST6) with carrying out salt containing half Hoagland nutrient solution of 4/5 artificial seawater salinity.
Above gradient experimental solutions is placed in test box, the tomato branch of having taken root is placed on box, root submerges in experimental solutions.Be that 25 1 DEG C, soil, light intensity are 25 in temperature
μmolm
-2s
-1, the photoperiod is to cultivate under the condition that 14h light/l0h is dark.The growing state of the each tomato variety of observed and recorded, determines the tolerance of salinity of each tomato variety.
(4) results and analysis
Result is as shown in table 3.Along with the increase of concentration of seawater, in identical incubation time, " fresh rich " tomato variety ST2 death, hands over No. 2 ST4 death of agriculture, hands over No. 1 ST5 death of agriculture, and Pan Nali tomato has wilting at ST6.Can determine by comparing the brine concentration adding in nutrient culture media the salt tolerant degree of these four tomato varieties, hand over No. 1, agriculture: Pan Nali tomato=2/3 artificial seawater salinity: 4/5 artificial seawater salinity=5/6 Pan Nali tomato salt-tolerant degree; Hand over No. 2, agriculture: Pan Nali tomato=1/2 artificial seawater salinity: 4/5 artificial seawater salinity=5/8 Pan Nali tomato salt-tolerant degree " fresh rich ": Pan Nali tomato=1/4 artificial seawater salinity: 4/5 artificial seawater salinity=5/16 Pan Nali tomato salt-tolerant degree.
Table 3 tomato variety salt tolerant summary sheet
| ? | CK | ST1 | ST2 | ST3 | ST4 | ST5 | ST6 |
| Pan Nali tomato | Normally | Normally | Normally | Normally | Normally | Normally | Wilt |
| Hand over No. 1, agriculture | Normally | Normally | Normally | Normally | Wilt | Dead | ? |
| Hand over No. 2, agriculture | Normally | Normally | Normally | Wilt | Dead | ? | ? |
| Fresh rich | Normally | Wilt | Dead | ? | ? | ? | ? |
(5) conclusion
Handing over No. 1 tolerance of salinity of agriculture is 5/6 of Pan Nali tomato, handing over No. 2 tolerance of salinitys of agriculture is 5/8 of Pan Nali tomato, " fresh rich " tomato salt-tolerant degree is 5/16 of Pan Nali tomato, using Pan Nali tomato as object of reference, just can judge very intuitively the salt tolerant degree of each tomato variety.In the present embodiment, tolerance of salinity order is: hand over No. 1 > of agriculture to hand over the fresh rich tomato of No. 2 > of agriculture.
Claims (5)
1. the fast appraisement method of a tomato salt-tolerant degree, it is characterized in that, it utilizes the wild-type tomato that the tolerance of salinity is very high---and Pan Nali tomato Solanum pennellii LA716 is as object of reference, by the salt tolerant degree of tomato material more to be evaluated and Pan Nali tomato, make quick judgement, concrete steps are as follows:
(1) plantation of Pan Nali tomato and tomato material to be evaluated;
(2) tissue of Pan Nali tomato and tomato material to be evaluated is cultivated or water planting;
(3) the salt tolerant gradient of Pan Nali tomato and tomato material to be evaluated experiment;
(4) the salt tolerant situation of tomato material to be evaluated and comparison and the evaluation of Pan Nali tomato; Wherein:
In described step (3), the concrete grammar of the salt tolerant gradient experiment of Pan Nali tomato and tomato material to be evaluated is: by CaCl
21.153g, MgCl
22.26g, MgSO
43.248g, KCl0.721g, NaCl26.726g are dissolved in distilled water, and constant volume to 1 liter, concentration artificial seawater is helped in preparation, use again distilled water diluting, be divided into 5-10 different gradients the two leaves plant wholeheartedly that grows into of Pan Nali tomato and tomato material to be evaluated is placed in each gradient artificial seawater and is cultivated.
2. fast appraisement method according to claim 1, it is characterized in that, in step (1), the concrete grammar that the plantation of described Pan Nali tomato and tomato material to be evaluated is taked is: the planting seed of Pan Nali tomato and tomato material to be evaluated, in the matrix of cave dish, is obtained to the plantling growing fine.
3. fast appraisement method according to claim 1, it is characterized in that, in step (2), the tissue of described Pan Nali tomato and tomato material to be evaluated is cultivated the concrete grammar of taking: clip increment identical with two leaves Pan Nali tomato wholeheartedly and the side shoot with blade of tomato material to be evaluated, after sterilization, under aseptic technique, be transplanted on the nutrient culture media of sterilizing and carry out culture of rootage.
4. fast appraisement method according to claim 1, it is characterized in that, in step (2), the concrete grammar that described Pan Nali tomato is taked with the water planting of tomato material to be evaluated is: clip increment identical with two leaves Pan Nali tomato wholeheartedly and the side shoot of tomato material to be evaluated, after wound disinfection, be placed on and in sterilized water, carry out culture of rootage.
5. fast appraisement method according to claim 1, it is characterized in that, in step (4), the salt tolerant situation of described tomato material to be evaluated from the concrete grammar that comparison and the evaluation of Pan Nali tomato taked is: the growth and mortality situation in different gradient artificial seawaters according to Pan Nali tomato and tomato material to be evaluated, each group experiment material is compared and evaluated, determine the tolerance of salinity of tomato material to be evaluated, the expression mode of the salt tolerant situation of tomato material to be evaluated is part Pan Nali tomato salt-tolerant degree.
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Cited By (4)
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| CN106069696A (en) * | 2016-07-07 | 2016-11-09 | 中国农业科学院棉花研究所 | Semi-wild is cotton to water planting Resistance Identification method in secondary saline and alkaline seedling stage |
| CN107941801A (en) * | 2017-12-29 | 2018-04-20 | 青岛袁策生物科技有限公司 | A kind of method of high frequency zone salt alkaline tolerance rice |
| CN107966434A (en) * | 2017-07-03 | 2018-04-27 | 扬州大学 | A kind of method of salt tolerance of eggplant Rapid identification |
| CN109362547A (en) * | 2018-11-15 | 2019-02-22 | 江苏沿江地区农业科学研究所 | A kind of identification method of semen viciae fabae salt tolerance |
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| CN1491537A (en) * | 2003-09-04 | 2004-04-28 | 上海交通大学 | Method for cultivating salt-resistant tomato plant |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069696A (en) * | 2016-07-07 | 2016-11-09 | 中国农业科学院棉花研究所 | Semi-wild is cotton to water planting Resistance Identification method in secondary saline and alkaline seedling stage |
| CN106069696B (en) * | 2016-07-07 | 2019-05-17 | 中国农业科学院棉花研究所 | Semi-wild cotton is to secondary saline and alkaline seedling stage water planting Resistance Identification method |
| CN107966434A (en) * | 2017-07-03 | 2018-04-27 | 扬州大学 | A kind of method of salt tolerance of eggplant Rapid identification |
| CN107941801A (en) * | 2017-12-29 | 2018-04-20 | 青岛袁策生物科技有限公司 | A kind of method of high frequency zone salt alkaline tolerance rice |
| CN109362547A (en) * | 2018-11-15 | 2019-02-22 | 江苏沿江地区农业科学研究所 | A kind of identification method of semen viciae fabae salt tolerance |
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Application publication date: 20140618 |