CN103314765A - Method for reproducing magnolia zenii seedlings - Google Patents
Method for reproducing magnolia zenii seedlings Download PDFInfo
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- CN103314765A CN103314765A CN2013102879860A CN201310287986A CN103314765A CN 103314765 A CN103314765 A CN 103314765A CN 2013102879860 A CN2013102879860 A CN 2013102879860A CN 201310287986 A CN201310287986 A CN 201310287986A CN 103314765 A CN103314765 A CN 103314765A
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Abstract
The invention provides a method for reproducing magnolia zenii seedlings. The method comprises the following steps that in June, a magnolia zenii branch which is half lignified and with a top leave is clipped by 8-10cm, and a half of a blade of the top leave is clipped; the lower portion of the magnolia zenii branch in the step (1) is immersed in rooting agent solution and then treated; the magnolia zenii branch in the step (2) is inserted to a cutting medium, Topsin medicinal liquid is used for spraying the cutting medium, and the cutting medium with the magnolia zenii branch is placed in a greenhouse with a sunshade net for culturing. The method is simple in process, branches with the proper lignification degree, kinds and concentration of rooting agents and proper cutting media are selected, the rooting ratio can reach more than 75%, therefore, the cutting survival ratio of the magnolia zenii seedlings is greatly improved, the magnolia zenii seedlings can be bred in a large-scale mode, the cost is low, and the efficiency is high.
Description
Technical field
The invention belongs to plant seedling breeding field, the particularly magnificent yulan sapling multiplication of a kind of treasured method.
Background technology
Precious magnificent yulan (Magnolia zenii) is the defoliation small arbor of angiosperm Magnoliaceae Magnolia, Jiangsu Province's endemic tree, and natural distribution is the precious Huashan in Jurong City only.The magnificent yulan of treasured is the representative of north subtropical zonal vegetation, is found in 1932 by the famous botanist Zheng's ten thousand jun of China, and names and make public, and is obvious with other yulans differences that " affiliation " arranged with it, and the classification of studying Magnolia is had important meaning.Precious magnificent yulan tree performance is tall and straight pretty simultaneously, and flower is dazzlingly beautiful, is the high rare deciduous species of ornamental value rare in the vegetable kingdom.
In 1991 " Chinese Plants Red Data Book "; precious magnificent yulan is listed in national III level endangered species; in August, 1999 State Council approved " national key protected wild plants register (first) " in; precious magnificent yulan is listed in national II level endangered species plant; classify the magnificent yulan of treasured as critical species in 2004 " Chinese Plants Red Lists "; in May, 2008 is by bio-diversity office of natural, ecological department of national environmental protection section; international wildlife conservation association's combined launch " Chinese each province represents the large-scale public welfare activities of biological competition "; choose on the net by the internet in June, 2009; precious magnificent yulan and Chinese tiger swallowtail are won as plant representative and the animal representative in Jiangsu respectively; become " the biological business card " in Jiangsu, and list the magnificent yulan of treasured in the plant " giant panda ".For protecting this rare tree species in imminent danger, successively set up nature reserve, the precious Huashan and National Park administration committee, the magnificent yulan of treasured is protected on the spot, protect its germ plasm resource.Protect the station field investigation that the magnificent yulan of treasured carries out to be shown with calendar year 2001 respectively at 2008 according to the Jiangsu Province wild animals and plants, the treelet of the adult individuality of 183 strains and hundreds of strains is arranged in the protection zone at present.Find in the investigation, although the broad leaved and deciduous broad leaved sylvan life has the precious magnificent yulan treelet of some, but because the strongly fragrant degree of covering of forest is higher, the sylvan life printing opacity is relatively poor, and its geographic range is narrow and small, and population at individual quantity is on the low side, be difficult to grow up to large tree, especially the plant that grows up is rare, and precious magnificent yulan population is involution form population age structure, now has been in endangered condition.Therefore, the magnificent yulan of treasured is introduced a fine variety, mass rearing is cultivated, and protects and utilize this rare plant resource imperative.
The shortage that is limited to germplasm resource not yet has pair research of the magnificent yulan of treasured abroad.The domestic system research report that it is expanded numerous and cultivation aspect that also has no.For many years, we explore sowing, cuttage and the propagation by grafiting technology of the magnificent yulan seedling of treasured successively.Existing sapling multiplication has three kinds of methods usually, and seed propagation, cottage propagation and propagation by grafiting are difficult to directly apply to the sapling multiplication of precious magnificent yulan.
The shortcoming performance of seed propagation: the one, provenance is rare, because precious magnificent yulan population at individual quantity is few, becomes to produce per year kind of tree still less, and seed maturity is subjected to taking food to seed collection of bird and mouse to bring larger difficulty; The 2nd, germination rate is low, because the hard ventilating permeable of precious magnificent yulan kind shell is poor, and the seed sprouting difficulty, the germination rate of seed only has 1-3% under the present condition; The 3rd, proterties produces variation, and the florescence is subjected to the impact of other yulan pollen, causes the variation of characters of progenies.Therefore, adopt seed propagation to be difficult to the magnificent yulan scale of treasured breeding.
The propagation by grafiting survival rate is extremely low.Owing to also not finding the yulan stock near with the magnificent yulan affiliation of treasured, that affinity good, survival rate is high at present, the large cost of the propagation by grafiting amount of labour used is higher simultaneously; Therefore propagation by grafiting is difficult to be applicable to the magnificent yulan scale of treasured breeding.
Adopt cuttage to breed precious magnificent yulan, its rooting rate is low, causes that the precious magnificent yulan cost of scale breeding is high, survival rate is low.
Summary of the invention
Goal of the invention: the purpose of this invention is to provide a kind of rooting rate precious magnificent yulan sapling multiplication method high, with low cost.
Technical scheme: the magnificent yulan sapling multiplication of a kind of treasured provided by the invention method may further comprise the steps:
(1) in June, the precious magnificent yulan branch of clip 8-10 ㎝ semi-lignified band top, top is cut and is stayed half blade;
(2) process in the precious magnificent yulan branch bottom immersion root-growing agent solution with step (1);
(3) the precious magnificent yulan branch after step (2) is processed inserts in the cutting medium, with topsin medicine liquid spray cutting medium, and has the cutting medium of precious magnificent yulan branch to place in the booth with sunshade net cuttage to cultivate, and get final product.
Wherein, in the step (2), described root-growing agent is ABT1 root-growing agent, NAA root-growing agent or IBA root-growing agent, preferred NAA root-growing agent, and described root-growing agent concentration of aqueous solution is 500-700mg/L, preferred 600mg/L, the processing time is 5-15s, preferred 10s.
Wherein, in the step (3), described cutting medium is the mixture of perlite, vermiculite and peat, and preferred volume ratio is (1-3): 1 perlite and the mixture of vermiculite, more preferably volume ratio is the perlite of 1:1 and the mixture of vermiculite.
Beneficial effect: the magnificent yulan sapling multiplication of treasured provided by the invention method technique is simple; by choosing the suitable branch of degree of lignification, root-growing agent kind and concentration, suitable cutting medium; rooting rate reaches about 75%; thereby greatly improved the cuttage survival rate of precious magnificent yulan seedling; thereby realize precious magnificent yulan seedling scale breeding, cost is low, efficient is high.
Particularly, the precious magnificent yulan Technique on Cuttage Propagation of rare tree species of the present invention compared with prior art has the following advantages:
1, survival rate is high: the present invention is by choosing the suitable branch of degree of lignification, the root-growing agent of selecting suitable concentration and suitable matrix, adopt the plastics cave dish cuttage, can reach the germination rate of taking root of 70-80%, and the germination rate of traditional seed propagation seed only has 1-3%, propagation by grafiting since stock affinity poor, survive few.Cross tender branch perishable, excessively old be difficult for taking root with the high branch of degree of lignification or rootage duration long, the present invention adopts the precious magnificent yulan branch in June, its spring tip stops growing, and is the branch of semi-lignified.
2, cost is low, the provenance material source is extensive: since branches for cuttage in the cutting orchard of setting up quantity greatly, easily obtain, the scale cuttage can be produced a large amount of seedlings.
3, be produced on a large scale: adopt method of the present invention; utilizing specification is the plastics cave dish in 28 ㎝ * 56 ㎝, 32 holes, and a wide 6M of long 75M takes up an area the steelframe booth of 577 ㎡, gets final product cuttage 6.4 Wan Zhibaohua yulan plugged ears; planting percent by 70%, a canopy can be produced 4.5 ten thousand strain caves dish seedling.
4, can keep precious magnificent yulan Different Varieties.The seed propagation of sexual propagation easily produces variation, and precious magnificent yulan is blooming when pollination, easily accepts the pollen of other yulan kind and offspring's variation occurs, and the inventive method adopts vegetative method, does not produce the character variation phenomenons such as kind of sexual involution.
5, the simple cost of production technology is low: in the steelframe booth with sunshade net, adopt the breeding of plastics cave dish and cutting medium, 10 months production cycle, the present cost of seedling is coiled about 0.5 yuan in every strain cave.
Embodiment
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand, the described concrete material proportion of embodiment, process conditions and result thereof only are used for explanation the present invention, and should also can not limit the present invention described in detail in claims.
Embodiment 1
In June, 2011, the researcher has carried out the correlation test of cuttage in Nanjing Forestry University, has selected the precious magnificent yulan branch A factor of ⑴, three levels: A1 life last year lignification branch; A2 gives birth to semi-lignified branch then; A3 gives birth to shoot then.⑵ root-growing agent kind B factor, three levels: the B1.ABT1(ABT1 root-inducing powder generally is used for the cuttage and seedling culture of plant with difficult rooting and rare species, member of Chinese Academy of Engineering professor Wang Tao development), B2.NAA (naa), B3.IBA(indolebutyric acid).⑶ root-growing agent concentration C factor, three levels: C1.400 ㎎/L, C2.600 ㎎/L, C3.800 ㎎/L).Carried out the orthogonal experiment of Three factors-levels.Matrix be perlite, vermiculite and peat each 1/3rd.
Result of the test shows: precious magnificent yulan is in the cuttage test, and is all the highest with its rooting rate of processing and the root system efficiency index of semi-lignified branch, NAA root-growing agent, 600 ㎎/L concentration (A2B2C2), is respectively 68% and 0.66; Treatment effect with semi-lignified branch, ABT1 root-growing agent, 600 ㎎/L concentration (A2B1C2) takes second place; Processing (A3 factor) the poorest branch with spray all rots; Time poor to give birth to branch, IBA root-growing agent, 400 ㎎/L treatment effect last year.
Embodiment 2
In June, 2012, the correlation test that the researcher has carried out again cuttage in the forest farm, Jurong City under the precious Huashan, the magnificent yulan of treasured protection zone has been selected ⑴ matrix (1. perlite, 2. vermiculite, 3. perlite: vermiculite=1: 1,4. perlite: peat=1: 1,5. perlite: vermiculite=3: 1,6. perlite: peat=3: 1,7. perlite: vermiculite: peat=1: 1: 1); ⑵ root-growing agent concentration (1. 400 ㎎/L, 2. 600 ㎎/L, 3. 800 ㎎/L); ⑶ in the processing time (1. 10s, 2. 10min, 3. 1h), carry out the cuttage test.Plugged ear is for giving birth to then semi-lignified branch, and root-growing agent is the NAA root-growing agent aqueous solution.
Result of the test shows: test in the investigation in 30 days in cuttage the magnificent yulan of treasured, take 3. perlite of matrix: vermiculite=1: 1, root-growing agent concentration 2. 600 ㎎/L, processing time 1. 10s as effect best, its rooting rate 62.22%, callus formation rate (not taking root) 17.78%, root system efficiency index 1.05.
Embodiment 3
Utilize method of the present invention to the magnificent yulan sapling multiplication of treasured, may further comprise the steps:
(1) in June, stop growing at the magnificent yulan of the treasured spring tip, when branch is in the semi-lignified degree, the precious magnificent yulan branch of clip 8-10 ㎝ semi-lignified band top, top is cut and is stayed half blade;
(2) process in the precious magnificent yulan branch bottom immersion root-growing agent solution with step (1); Described root-growing agent solution is NAA root-growing agent solution, with NAA root-growing agent pulvis first with the dissolving of the alcohol of 75% above concentration, to be mixed with concentration again be 500-700 ㎎/LNAA root-growing agent aqueous solution;
(3) the precious magnificent yulan branch after step (2) processing being inserted the interior processing time of cutting medium is 5-15s, with topsin medicine liquid spray cutting medium, prevent that the plugged ear wound infection is downright bad, and have the cutting medium of precious magnificent yulan branch to place in the booth with sunshade net cuttage to cultivate, and get final product; Wherein cutting medium is that volume ratio is (1-3): 1 perlite and the mixture of vermiculite, cutting medium have added water to moist feeling and have held not water outlet and be advisable; Described topsin liquid is that 1000 times of 50% topsin dilutions make by mass percent concentration.
Its rooting rate is 75.2%, and the root system efficiency index is 1.09.
Claims (3)
1. the magnificent yulan sapling multiplication of treasured method is characterized in that: may further comprise the steps:
(1) in June, the precious magnificent yulan branch of clip 8-10 ㎝ semi-lignified band top, top is cut and is stayed half blade;
(2) process in the precious magnificent yulan branch bottom immersion root-growing agent solution with step (1);
(3) the precious magnificent yulan branch after step (2) is processed inserts in the cutting medium, with topsin medicine liquid spray cutting medium, and has the cutting medium of precious magnificent yulan branch to place in the booth with sunshade net cuttage to cultivate, and get final product.
2. the magnificent yulan sapling multiplication of a kind of treasured according to claim 1 method, it is characterized in that: in the step (2), described root-growing agent is ABT1 root-growing agent, NAA root-growing agent or IBA root-growing agent, described root-growing agent concentration of aqueous solution 400-800mg/L, and the processing time is 5-15s.
3. the magnificent yulan sapling multiplication of a kind of treasured according to claim 1 method, it is characterized in that: in the step (3), described cutting medium is the mixture of perlite, vermiculite and peat.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104429627A (en) * | 2014-10-14 | 2015-03-25 | 深圳文科园林股份有限公司 | Huodendrontibeticumcuttage method |
| CN104488678A (en) * | 2014-12-10 | 2015-04-08 | 广德诚源花卉苗木种植家庭农场 | Lily magnolia cuttage sapling cultivation method and foundation soil formula of lily magnolia cuttage sapling |
| CN105638220A (en) * | 2016-02-09 | 2016-06-08 | 叶鸿钊 | Cutting propagation method for magnolia soulangeana |
| CN106105980A (en) * | 2016-06-28 | 2016-11-16 | 广西南宁十分园生态农业科技有限公司 | A kind of Magnolia liliiflora cuttage and seedling culture method |
| CN106613701A (en) * | 2016-12-29 | 2017-05-10 | 王文龙 | Magnolia liliiflora cutting seedling culturing technology |
| CN106717953A (en) * | 2016-12-26 | 2017-05-31 | 安徽圆梦建筑有限公司 | A kind of blue implantation methods of carbuncle |
| CN108260459A (en) * | 2018-01-24 | 2018-07-10 | 北京林业大学 | A kind of Magnolia wufengensis softwood cutting propagation method |
| CN111448988A (en) * | 2020-05-18 | 2020-07-28 | 江苏农林职业技术学院 | Culture method and culture medium for inhibiting endophyte pollution in primary culture of magnolia zenii |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104429627A (en) * | 2014-10-14 | 2015-03-25 | 深圳文科园林股份有限公司 | Huodendrontibeticumcuttage method |
| CN104488678A (en) * | 2014-12-10 | 2015-04-08 | 广德诚源花卉苗木种植家庭农场 | Lily magnolia cuttage sapling cultivation method and foundation soil formula of lily magnolia cuttage sapling |
| CN105638220A (en) * | 2016-02-09 | 2016-06-08 | 叶鸿钊 | Cutting propagation method for magnolia soulangeana |
| CN106105980A (en) * | 2016-06-28 | 2016-11-16 | 广西南宁十分园生态农业科技有限公司 | A kind of Magnolia liliiflora cuttage and seedling culture method |
| CN106717953A (en) * | 2016-12-26 | 2017-05-31 | 安徽圆梦建筑有限公司 | A kind of blue implantation methods of carbuncle |
| CN106613701A (en) * | 2016-12-29 | 2017-05-10 | 王文龙 | Magnolia liliiflora cutting seedling culturing technology |
| CN108260459A (en) * | 2018-01-24 | 2018-07-10 | 北京林业大学 | A kind of Magnolia wufengensis softwood cutting propagation method |
| CN111448988A (en) * | 2020-05-18 | 2020-07-28 | 江苏农林职业技术学院 | Culture method and culture medium for inhibiting endophyte pollution in primary culture of magnolia zenii |
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Application publication date: 20130925 |