CN110771365B - Cutting propagation method of lingbao rhododendron - Google Patents

Cutting propagation method of lingbao rhododendron Download PDF

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Publication number
CN110771365B
CN110771365B CN201911016406.8A CN201911016406A CN110771365B CN 110771365 B CN110771365 B CN 110771365B CN 201911016406 A CN201911016406 A CN 201911016406A CN 110771365 B CN110771365 B CN 110771365B
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rhododendron
concentration
lingbao
cutting
soaking treatment
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CN110771365A (en
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彭万喜
董帅伟
张党权
郑东方
赵勇
毕会涛
姚冠忠
李项群
张亚芳
赵毅
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Henan Xiaoqinling National Nature Reserve Administration State Owned Sanmenxia Hexi Forest Farm
Henan Agricultural University
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Henan Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/02Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
    • A01G9/029Receptacles for seedlings
    • A01G9/0299Handling or transporting of soil blocks or seedlings

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cultivation Of Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The embodiment of the invention provides a cutting propagation method of lingbao rhododendron, which saves seeds, has simple and convenient operation steps, short seedling culture period, large number of seedlings which can be cultured per unit area, short seedling revival period after field planting, high survival rate and improved propagation efficiency.

Description

Cutting propagation method of lingbao rhododendron
Technical Field
The invention belongs to the technical field of artificial plant breeding, and particularly relates to a cutting propagation method of lingbao rhododendron.
Background
The rhododendron is a shrub, the arbor is very rare, and the lingbao rhododendron belongs to an arbor, is a variety of rhododendron and is sparsely distributed in nature. The lingbao rhododendron is different from the common Henan rhododendron in that the leaves of the variety are thin leather, the shape of the variety is oval or inverted oval, the length of the variety is 5-9 cm, the width of the variety is 2.5-4.5 cm, the variety is small, the length of the crown of the variety is only 2.5 cm, no dark spots exist, and the like, and the flowering period is from 5 middle of the month to 6 middle of the month.
The lingbao rhododendron has tall and straight growth vigor, and some of the lingbao rhododendron can be as high as twenty-thirty meters, the root of a dish is staggered, and legendary small dragon with horns branches are vigorous. The existing lingbao rhododendron with the longest age has been around 1300 years, the chest circumference is about 1.45 meters, the height is 8 meters, the crown width is 8 meters, the crown is umbrella-shaped, and the lingbao rhododendron still flowers and leaves. The flower buds of the lingbao rhododendron are peach-red, most of the flowers are white, a little pink, and the flowers are large, and a round flower crown with the size of a fist is formed by clustering more than ten small flowers, so that the ornamental value is high.
Because the lingbao rhododendron is sparsely distributed in nature and only distributed in a specific area, the development and utilization of the biological and ornamental values of the lingbao rhododendron are severely restricted, and the existing conventional rhododendron propagation technology is not suitable for the lingbao rhododendron.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art. Therefore, the cutting propagation method of the lingbao rhododendron is simple and convenient in operation steps, short in seedling culture period and high in survival rate.
The cutting propagation method of the lingbao rhododendron according to the embodiment of the invention comprises the following steps:
s1: picking tender branches of the lingbao rhododendron, and soaking the tender branches of the lingbao rhododendron with disinfectant;
s2: carrying out pruning treatment on the tender branches of the lingbao rhododendron treated in the step S1;
s3: soaking the rhododendron dauricum twigs treated in the step S2 with a rooting agent in a gradient concentration manner;
s4: and E, cutting the tender branches of the rhododendron lingbao treated in the step S3.
The cutting propagation method of the lingbao rhododendron according to the embodiment of the invention at least has the following technical effects:
the cutting propagation method of the embodiment of the invention saves seeds, has simple and convenient operation steps, short seedling culture period, large number of seedlings which can be cultured per unit area, short seedling revival period after field planting and high survival rate, and improves the propagation efficiency.
In the embodiment of the invention, the seedlings are bred by cutting the tender branches of the lingbao rhododendron, the root quantity is large, the root system not only grows around the cut, but also explosive adventitious roots can grow under the surface skin of the tender branches.
In the cutting propagation method of the embodiment of the invention, the potted plant cutting and the arched film shed cutting can be selected according to the conditions in the cutting mode, so that the method is easy to popularize and apply.
In step S1:
when picking the tender branches of the rhododendron lingeri, selecting clear weather, and picking the tender branches of the sunny branches by using high branch scissors between 9 and 11 am, wherein the length of the branches is 30-50 cm. The tender branches of the lingbao rhododendron are soaked by using a disinfectant, so that the purpose is to prevent the incision from being infected by germs, and the incision cannot root because of the occurrence of diseases such as black stems and the like.
According to some embodiments of the invention, the disinfecting liquid in the step S1 is a 3-5 vol% potassium permanganate solution.
According to some embodiments of the present invention, the soaking time in step S1 is 1-2 min.
In step S2:
pruning the tender branches of the lingbao rhododendron processed in the step S1, and immediately processing the collected tender branches of the lingbao rhododendron to remove old branches, old leaves and diseased leaves. After the pruning treatment, the subsequent rooting agent treatment needs to be carried out within 24 hours.
According to some embodiments of the invention, the pruning processing in step S2 is performed by: building short branches of 5-10 cm long from the tender branches of the rhododendron lingeri, reserving 2 leaves for each short branch, and cutting half area of each leaf.
In step S3:
when the rhododendron superbum twigs treated in the step S2 are subjected to gradient concentration soaking treatment by using the rooting agent, the rhododendron superbum twigs are soaked in the high-concentration rooting agent for a short time, then soaked in the medium-concentration rooting agent for a long time, and finally soaked in the low-concentration rooting agent for a long time, so that the shoots can be effectively stimulated to root, and the rooting rate is improved.
According to some embodiments of the invention, the rooting agent of step S3 is one of IBA and IAA.
According to some embodiments of the present invention, the temperature of the gradient concentration soaking treatment in the step S3 is 25-32 ℃.
According to some embodiments of the present invention, the gradient concentration soaking treatment comprises a first concentration soaking treatment, a second concentration soaking treatment and a third concentration soaking treatment which are sequentially performed, wherein the concentration of the rooting agent in the first concentration soaking treatment is 2.5-3.0 g/L, the concentration of the rooting agent in the second concentration soaking treatment is 1.0-2.0 g/L, and the concentration of the rooting agent in the third concentration soaking treatment is 0.5-1.0 g/L.
According to some embodiments of the invention, the first concentration soaking treatment is performed for 1-2 min.
According to some embodiments of the invention, the second concentration soaking treatment is performed for 30-60 min.
According to some embodiments of the invention, the third concentration soaking treatment is performed for 1-6 hours.
According to some embodiments of the invention, the cuttage of step S4 includes potted cuttage and arched thin film shed cuttage.
And (3) potted plant cuttage, wherein 2 plants are planted in each pot, the cuttage substrate is a mixture of sandy soil, organic fertilizer and vermiculite, the mass ratio is 7:2:1, and the rooting is carried out for about 50-70 days.
And (3) cutting in an arched film shed, wherein a cutting medium is sand and organic fertilizer, the mass ratio is 20:1, the cutting density is 10 multiplied by 10cm, and the root is taken in about 40-60 days.
Detailed Description
The following are specific examples of the present invention, and the technical solutions of the present invention will be further described with reference to the examples, but the present invention is not limited to the examples.
Example 1
The embodiment provides a cutting propagation method of rhododendron lingbao, which comprises the following steps:
s1: picking tender branches of the lingbao rhododendron, and soaking the tender branches of the lingbao rhododendron with disinfectant;
s2: carrying out pruning treatment on the tender branches of the lingbao rhododendron treated in the step S1;
s3: soaking the rhododendron dauricum twigs treated in the step S2 with a rooting agent in a gradient concentration manner;
s4: and E, cutting the tender branches of the rhododendron lingbao treated in the step S3.
In step S1:
when picking the tender branches of the rhododendron lingeri, selecting clear weather, and picking the tender branches of the sunny branches by using high branch scissors between 9 and 11 am, wherein the length of the branches is 30-50 cm. The tender branches of the lingbao rhododendron are soaked by using a disinfectant, so that the purpose is to prevent the incision from being infected by germs, and the incision cannot root because of the occurrence of diseases such as black stems and the like. The disinfectant is a potassium permanganate solution with the volume fraction of 3-5%. The soaking time is 1-2 min.
In step S2:
pruning the tender branches of the lingbao rhododendron processed in the step S1, and immediately processing the collected tender branches of the lingbao rhododendron to remove old branches, old leaves and diseased leaves. After the pruning treatment, the subsequent rooting agent treatment needs to be carried out within 24 hours. The pruning treatment method comprises the following steps: building short branches of 5-10 cm long from the tender branches of the lingbao rhododendron, reserving 2 leaves for each short branch, and cutting half area of each leaf.
In step S3:
when the rhododendron superbum twigs treated in the step S2 are subjected to gradient concentration soaking treatment by using the rooting agent, the rhododendron superbum twigs are soaked in the high-concentration rooting agent for a short time, then soaked in the medium-concentration rooting agent for a long time, and finally soaked in the low-concentration rooting agent for a long time, so that the shoots can be effectively stimulated to root, and the survival rate is improved. The rooting agent is one of IBA and IAA. The temperature of the gradient concentration soaking treatment is 25-32 ℃. The gradient concentration soaking treatment comprises a first concentration soaking treatment, a second concentration soaking treatment and a third concentration soaking treatment which are sequentially carried out, wherein the concentration of the rooting agent in the first concentration soaking treatment is 2.5-3.0 g/L, the concentration of the rooting agent in the second concentration soaking treatment is 1.0-2.0 g/L, and the concentration of the rooting agent in the third concentration soaking treatment is 0.5-1.0 g/L. The time of the first concentration soaking treatment is 1-2 min. The time of the second concentration soaking treatment is 30-60 min. The time of the third concentration soaking treatment is 1-6 h.
In step S4:
the cuttage comprises potted plant cuttage and arched film shed cuttage.
And (3) potted plant cuttage, wherein 2 plants are planted in each pot, and the cuttage substrate is a mixture of sandy soil, an organic fertilizer and vermiculite, and the mass ratio is 7:2: 1.
The cuttage is carried out in an arched film shed, the cuttage substrate is sand and organic fertilizer, the mass ratio is 20:1, and the cuttage density is 10 multiplied by 10 cm.
Example 2
The example carries out cutting propagation on 30 lingbao rhododendron twigs, and the steps comprise:
s1: picking tender branches of the lingbao rhododendron, and soaking the tender branches of the lingbao rhododendron with disinfectant;
s2: carrying out pruning treatment on the tender branches of the lingbao rhododendron treated in the step S1;
s3: soaking the rhododendron dauricum twigs treated in the step S2 with a rooting agent in a gradient concentration manner;
s4: and E, cutting the tender branches of the rhododendron lingbao treated in the step S3.
In step S1:
when picking the tender branches of the rhododendron lingeri, selecting clear weather, and picking the tender branches of the sunny branches by using high branch scissors between 9 and 11 am, wherein the length of the branches is 30-50 cm. The tender branches of the lingbao rhododendron are soaked by using a disinfectant, so that the purpose is to prevent the incision from being infected by germs, and the incision cannot root because of the occurrence of diseases such as black stems and the like. The disinfectant is a potassium permanganate solution with the volume fraction of 4 percent. The soaking time is 2 min.
In step S2:
pruning the tender branches of the lingbao rhododendron processed in the step S1, and immediately processing the collected tender branches of the lingbao rhododendron to remove old branches, old leaves and diseased leaves. After the pruning treatment, the subsequent rooting agent treatment needs to be carried out within 24 hours. The pruning treatment method comprises the following steps: constructing 10 cm-long short branches of Rhododendron Laevigata, reserving 2 leaves for each short branch, and cutting half area of each leaf.
In step S3:
when the rhododendron superbum twigs treated in the step S2 are subjected to gradient concentration soaking treatment by using the rooting agent, the rhododendron superbum twigs are soaked in the high-concentration rooting agent for a short time, then soaked in the medium-concentration rooting agent for a long time, and finally soaked in the low-concentration rooting agent for a long time, so that the shoots can be effectively stimulated to root, and the survival rate is improved. The rooting agent is IBA. The temperature of the gradient concentration soaking treatment was 25 ℃. The gradient concentration soaking treatment comprises a first concentration soaking treatment, a second concentration soaking treatment and a third concentration soaking treatment which are sequentially carried out, wherein the concentration of the rooting agent in the first concentration soaking treatment is 3.0g/L, the concentration of the rooting agent in the second concentration soaking treatment is 2.0g/L, and the concentration of the rooting agent in the third concentration soaking treatment is 1.0 g/L. The time of the first concentration soaking treatment was 1.5 min. The time for the second concentration soaking treatment was 45 min. The time of the third concentration soaking treatment is 3 h.
In step S4:
the cuttage is the cuttage in an arched film shed, the cuttage substrate is sand and organic fertilizer, the mass ratio is 20:1, and the cuttage density is 10 multiplied by 10 cm.
Example 3
In this example, the basic steps of cutting propagation of 30 linglingbao rhododendron shoots are the same as in example 2, except that in step S3:
when the rhododendron superbum twigs treated in the step S2 are subjected to gradient concentration soaking treatment by using the rooting agent, the rhododendron superbum twigs are soaked in the high-concentration rooting agent for a short time, then soaked in the medium-concentration rooting agent for a long time, and finally soaked in the low-concentration rooting agent for a long time, so that the shoots can be effectively stimulated to root, and the survival rate is improved. The rooting agent is IBA. The temperature of the gradient concentration soaking treatment was 25 ℃. The gradient concentration soaking treatment comprises a first concentration soaking treatment, a second concentration soaking treatment and a third concentration soaking treatment which are sequentially carried out, wherein the concentration of the rooting agent in the first concentration soaking treatment is 2.5g/L, the concentration of the rooting agent in the second concentration soaking treatment is 1.0g/L, and the concentration of the rooting agent in the third concentration soaking treatment is 0.5 g/L. The time of the first concentration soaking treatment was 1.5 min. The time for the second concentration soaking treatment was 45 min. The time of the third concentration soaking treatment is 3 h.
Example 4
In this example, the basic steps of cutting propagation of 30 linglingbao rhododendron shoots are the same as in example 2, except that in step S4:
the cuttage is potted plant cuttage, 2 plants are planted in each pot, and the cuttage substrate is a mixture of sandy soil, organic fertilizer and vermiculite, and the mass ratio is 7:2: 1.
Example 5
In this example, 30 linglingbao rhododendron shoots were subjected to cutting propagation, and the basic procedure was the same as in example 2, except that in step S3, the rooting agent was IAA.
Comparative example 1
In this example, 30 linglingbao rhododendron shoots were subjected to cutting propagation, and the basic procedure was the same as in example 2, except that in step S3, the gradient concentration soaking treatment was not performed. Step S3 specifically includes:
and (4) soaking the rhododendron jujuveniles treated in the step (S2) with a rooting agent in a short time, wherein the concentration of the rooting agent is 3.0 g/L. The soaking time is 1.5 min.
Comparative example 2
In this example, 30 linglingbao rhododendron shoots were subjected to cutting propagation, and the basic procedure was the same as in example 2, except that in step S3, the gradient concentration soaking treatment was not performed. Step S3 specifically includes:
and (4) soaking the rhododendron jujuveniles treated in the step (S2) with a rooting agent with a medium concentration of 2.0g/L for a little long time. The soaking time is 45 min.
Comparative example 3
In this example, 30 linglingbao rhododendron shoots were subjected to cutting propagation, and the basic procedure was the same as in example 2, except that in step S3, the gradient concentration soaking treatment was not performed. Step S3 specifically includes:
and (4) soaking the rhododendron jujubes processed in the step (S2) with a rooting agent with a low concentration for a long time, wherein the concentration of the rooting agent is 1.0 g/L. The soaking time is 3 h.
Example of detection
In the example, after the rhododendron superbum tender branch cutting propagation of the examples 2 to 5 and the comparative examples 1 to 3 is observed and recorded, the rooting time and the rooting condition are observed for the first time, and for the rooted branches, the growth condition and the survival rate of the branches are also observed after half a year, and the specific results are shown in table 1.
TABLE 1
Figure BDA0002245845270000071

Claims (6)

1. A cutting propagation method of rhododendron lingbao is characterized by comprising the following steps:
s1: picking tender branches of the lingbao rhododendron, and soaking the tender branches of the lingbao rhododendron with disinfectant;
s2: carrying out pruning treatment on the tender branches of the lingbao rhododendron treated in the step S1;
s3: soaking the rhododendron dauricum twigs treated in the step S2 with a rooting agent in a gradient concentration manner;
s4: cutting the tender branches of the lingbao rhododendron treated in the step S3;
the gradient concentration soaking treatment comprises a first concentration soaking treatment, a second concentration soaking treatment and a third concentration soaking treatment which are sequentially carried out, wherein the concentration of a rooting agent in the first concentration soaking treatment is 3.0g/L, the concentration of a rooting agent in the second concentration soaking treatment is 2.0g/L, and the concentration of a rooting agent in the third concentration soaking treatment is 1.0 g/L;
the time of the first concentration soaking treatment is 1.5 min;
the time of the second concentration soaking treatment is 45 min;
the time of the third concentration soaking treatment is 3 hours.
2. The cutting propagation method of rhododendron linguense according to claim 1, wherein the disinfectant in the step S1 is 3-5% by volume potassium permanganate solution.
3. The cutting propagation method of rhododendron linguense according to claim 1, wherein the pruning treatment in step S2 is as follows: and (3) cutting the tender branches of the rhododendron lingeri into short branches with the length of 5-10 cm, reserving 2 leaves for each short branch, and cutting half area of each leaf.
4. The cutting propagation method of rhododendron linguense according to claim 1, wherein the rooting agent in step S3 is one of IBA and IAA.
5. The cutting propagation method of rhododendron superbum according to claim 1, wherein the temperature of the gradient concentration soaking treatment in step S3 is 25-32 ℃.
6. The cutting propagation method of rhododendron linguense as claimed in claim 1, wherein the cutting in step S4 comprises potted cutting and arched film shed cutting.
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CN112889393A (en) * 2021-01-21 2021-06-04 洛阳师范学院 Method for artificially breeding seedlings of rare or endangered plant lingbao rhododendron seeds
CN115119639A (en) * 2022-07-10 2022-09-30 山东农业大学 Single-bud extremely-short-shoot green branch cuttage method for rhododendron delavayi

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